Inom offentliga organisationer har det tidigare inte funnits en lag som tillåtit automatiserat beslutsstöd, det var först år 2018 som lagen förändrades. Tidigare forskning har fokuserat på automation inom privata näringslivet, vilket skapat en brist på forskning för offentliga organisationer. Efter lagförändringen har socialförvaltningar börjat automatisera beslutsunderlaget för försörjningsstöd i sina beslutsstödsprocesser. Automatiseringen sker genom användningen av mjukvarutekniken RPA (robotstyrd processautomation). RPA kan enbart hantera processer som är regelstyrda och strukturerade. Det tvingar socialförvaltningarna att definiera och strukturera upp sina beslutsstödsprocesser som tidigare utfördes manuellt av handläggarna. Det innefattar att konsekvenser uppstår och påverkar beslutsstödsprocessen, vilket leder fram till studiens frågeställning: Hur påverkas beslutsstödsprocessen i kommunala förvaltningar genom användningen av RPA? En kvalitativ metod har använts vid insamling av litteratur och empiri, för att kunna identifiera konsekvenserna och dess påverkan i beslutsstödsprocessen. De identifierade konsekvenserna som presenteras i studiens slutsats är uppdelade efter: Konsekvenser utifrån RPA och Konsekvenser utifrån handläggarna. Konsekvenserna som identifierats i studien har skiljt sig åt mellan de kommunala förvaltningarna, beroende på hur lagen har tolkats eller om RPA har börjat användas i kommunerna än. / In communal organizations there has previously not existed a law that allowed automated decision support, it was during the year 2018 that the law changed. Previous research has focused on automation in the private business sector, which has created a lack of research on public organizations. Following the change in the law, Social services have started to automate the decision support in their decision support processes. The automation takes place using the software technology RPA (Robot Process Automation). RPA can only handle processes that are regulated and structured. It forces the Social services to define and structure their decision support processes that were previously manually performed by administrators. It involves creation of consequences that affects the decision support process and leads to the study ́s question: How is the decision support process in communal organizations affected using RPA? A qualitative method has been used in the collection of literature and empirical data, in order to be able to identify the consequences and its impact in the decision support process. The identified consequences presented in the study's conclusion are divided into the categories: consequences based on RPA and consequences based on the administrators. The consequences identified in the study have differed between the communal organizations, depending on how they interpreted the law and whether RPA has started to be used in the communes yet.

In wildlife management, using cutting edge technology and science to monitor greater sage-grouse (Centrocercus urophasianus; sage-grouse) populations, enables land managers to better assess the impact of their management decisions. Having precise counts of sage-grouse lek attendance, and specifically male lek attendance, is an important metric used to evaluate population status and response to conservation actions (Gifford et.al, 2013, Dahlgren et al., 2016). Leks are seasonal breeding sites where males perform a ritualistic courtship dance for females. Our case study examined if a Remotely Piloted Aircraft (RPA) was effective in detecting, and counting, sage-grouse during the lek season (early March to late April). More specifically, this research used a Forward-Looking Infrared (FLIR) camera (a thermal camera) to detect sage-grouse and determine body temperatures of individual sage-grouse to determine if temperature data can be used to identify displaying male sage-grouse. These images can be used to document the activity and behavior of sage-grouse and can be revisited at future times to document changes in bird numbers as well as perform additional statistical analyses. We conducted 5 flights and on a per-flight basis, we identified an average of 4.4 displaying males, 13.4 non-displaying males, and 5.6 female sage-grouse. We found that the average size and average maximum temperature of the three sage-grouse categories differed where females were smaller with an average body size of 325 cm2, an average maximum temperature of 14.6 C ̊, and a smaller average thermal range of 2.47 C ̊. Non-displaying male body size was approximately 488 cm2, with a maximum average temperature of 17.2 C ̊, and an average thermal range of 4.66C ̊. Displaying male body size was the largest at approximately 655 cm2, an average maximum temperature of 27.5C ̊, with the largest average range of 12.39C ̊. Our study demonstrates that RPA and infrared technology can be used to conduct accurate sage-grouse lek attendance counts. Further, results of this study will also provide a guideline for the use of RPA’s to monitor sage-grouse and other lekking species.

No description available.

<p>The eukaryotic genome integrity is safeguarded by the DNA damage response, which is composed of a network of signal transduction pathways that upon genotoxic stresses, arrest cell cycle progression, motivate repair processes, or induce apoptosis or senescence when cells incur irreparable DNA damage. During this process, DNA damage-induced post-translational modifications, most notably protein phosphorylation, of a variety of DNA damage-responsive proteins has been shown to mediate the initiation, transduction and reception of the DNA damage signals, resulting in alterations of their stability, activities or subcellular localizations, ultimately leading to activation of various downstream effector pathways. </p><p>While a lot has been elucidated on the downstream events of the DNA damage response, little is known about how DNA damage is detected. Two still ongoing studies of this dissertation attempt to address this question. Our preliminary work on ATM indicates that serine 2546 is critical for its kinase activity. Substitution of this residue with phosphomimetic aspartate, but not nonphosphorylable alanine, abrogates the kinase activity of ATM and fails to rescue the checkpoint-deficient phenotype exhibited by the ATM-deficient cells, suggesting that removal of an inhibitory phospho group at S2546 might be required for the activation of ATM. In another study, we identified a novel DNA-damage responsive threonine residue (T622) in Rad17, which undergoes ATM/ATR-dependent phosphorylation in vitro and in vivo. Ectopic expression of a phosphodeficient mutant (T622A) of Rad17, but not its wild-type control, shows a pronounced defect in sustaining Chk1 phosphorylation and the corresponding G2/M checkpoint upon DNA damage, suggesting that phosphorylation at T622 might complement that on the two previously reported phosphorylation sites, S635 and S645, to mediate G2/M checkpoint activation while the latter is primarily responsible for intra-S phase checkpoint. </p><p>Although a large amount of knowledge has been accumulated about the initiation and activation process of the DNA damage response, how cells recover, the equally important flip side of the response, has remained poorly understood. We have found that in cells recovering from replication stress, RPA32 phosphorylation at ATM/ATR-responsive sites T21 and S33, which reportedly suppresses DNA replication and recruiting other checkpoint and repair proteins to the DNA lesions, is reversed by the serine/threonine protein phosphatase 2A (PP2A). Cells with a RPA32 persistent-phosphorylation mimic (T21D/S33D) exhibit normal checkpoint activation and re-enter the cell cycle normally after recovery, but display a pronounced defect in the repair of DNA breaks. These data indicate that PP2A-mediated RPA32 dephosphorylation may be a required event during the repair process in the DNA damage response. </p><p>In summary, these studies in this dissertation highlight the importance of reversible phosphorylation and dephosphorylation in the modulation of the DNA damage response. What's more, they also extend our knowledge and deepen our understanding of this process by revealing that dephosphorylation may positively regulate the activation of cell cycle checkpoints, which is seemingly dominated by protein phosphorylation upon DNA damage, that phosphorylation of certain checkpoint proteins at different sites may result in distinct consequences, and that dephosphorylation of some activated checkpoint/repair proteins may function as an important mechanism for cells to recover from the DNA damage response.</p> / Dissertation

No description available.

Homologous recombination (HR) repair is critical for the maintenance of genomic stability, as it is involved in the precise repair of DNA double-strand breaks (DSBs) using an intact homologous template for repair. The initiation of 5' strand resection of DNA ends is a critical determinant in this process, which commits cells to HR repair and prevents repair by non-homologous end joining (NHEJ). The human single-stranded DNA (ssDNA) binding complexes, RPA and SOSS1, are involved in regulating DSB signaling and HR repair. In this study, I demonstrate a novel function of SOSS1 in HR repair, in which SOSS1 stimulates hExo1-dependent resection. Despite its poor activity in binding duplex DNA, SOSS1 facilitates hExo1 recruitment to duplex DNA ends and promotes its activity in resection independently of MRN in vitro. MRN(X) is a highly conserved complex that is involved in the early steps of HR repair by regulating DSB resection. MRN interacts with CtIP and constitutes resection machinery that can perform limiting processing on DNA ends. In this study, I also examine the biochemical activities of MRN and CtIP in DSB resection through reconstituted in vitro assays. I show that the ATP-dependent DNA unwinding activity of MRN is responsible for overcoming Ku inhibition of hExo1- and Dna2/BLM-dependent resection activity in vitro. I propose that this unwinding step displaces Ku away from the DNA ends and facilitates the recruitment of hExo1 to the DNA ends for efficient resection. In addition, I show that CtIP can promote overcoming the inhibitory effect of Ku in resection together with MRN. Further, I demonstrate that MRN nuclease activity is required for efficient processing of covalent adducts from DNA ends in vitro, suggesting that the nucleolytic removal of covalent adducts by MRN generates free ends for hExo1- and Dna2/BLM binding. Overall, this study provides mechanistic insight into the regulation of DSB resection in human cells. / text

This study investigates the intra-organisational adoption of technological innovation and focuses specifically on the perceived influence of innovation, organizational, individual and managerial facilitation factors on the adoption process of Robotic process automation (RPA) technology. Based on an exploration and integration of eight adoption related frameworks from Innovation diffusion, User acceptance and Change management fields, a conceptual model linking the system level change to individual level commitment process was created. The model integrates concepts from Diffusion of Innovation, Theory of Reasoned Action, Theory of Planned Behaviour, Technology Acceptance Model, Unified Theory of Acceptance and Use, Lewin’s 3 stage-model, Kotter’s 8-step model and Change Institutionalization framework. A qualitative case study at Stora Enso’s Finance delivery function was conducted utilizing semi-structured interviews to contribute to better understanding about the adoption process of RPA. The empirical data was then analysed against the created conceptual model. The findings from the case study suggest that contextual factors related to innovation, organizational, individual and managerial facilitation attributes are perceived to influence the adoption of RPA. In relation to innovation attributes the key themes identified were RPA advantages, risks and threats, ease of understanding and use, development effort and RPA-process fit. Under the category of organizational attributes, the main factors identified were RPA-organization fit, previous RPA experience, RPA capabilities and organizational support. Key individual attribute related factors were personality, time resources, previous success with RPA and personal motivation, and main managerial facilitation attributes were management support, communication, knowledge and experience sharing, training and skill development and employee involvement.

Automatisering av arbetsprocesser har varit ett aktuellt tema för många verksamheter i många år. Ofta är dessa automatiseringar relaterade till fysisk automation som exempelvis robotar vid produktionslinjer. Men med dagens teknik finns det potential för att automatisera arbetsprocesser i verksamheters back-office processer. Robotic process automation, eller förkortat som RPA, är en teknik som har förmågan att imitera användarens steg i en arbetsprocess och återskapa arbetsprocessen utan den mänskliga resursen. Denna studie har som syfte att fastställa utmaningar och möjligheter som är relaterade vid införande av RPA i verksamheter. Genom intervjuer och tidigare publicerat material om RPA, skall denna studie erbjuda en djupare förståelse kring utmaningar och möjligheter för den stigande trenden robotic process automation. / Automation has been a hot topic for many businesses for a number of years now. The term is usually related to automation of physical machinery like robots to speed up production and assembling of cars. But with currently technology, the automation has taken a step further. Automation is now capable of automating back-office processes. Robotic Process Automation, also known as RPA, is a technology that is capable of imitating the users step in a business process and replicate it automatically, without human intervention. The purpose of this study is to determine the challenges and opportunities related to the implementation of RPA in businesses. By performing interviews and examining existing material about RPA, this study aims to deliver a deeper and wider understanding of the rising technology that is Robotic Process Automation.

Background: Businesses today are transforming its financial and operative processes in order to survive on the market. The human interaction of the standardised work tasks is being replaced by Robotics Process Automation (RPA) in order to make the finance processes less costly and more time efficient. Thus, human employees are delegated more qualitative work tasks and their behaviour as well as knowledge are in need to be changed. In this process, barriers may occur, and managers need to make their employees adapt in a comprehensive way to overcome these.  Purpose: The purpose of this thesis is to find adaptation barriers that occur when the finance professionals are adapting to more qualitative work tasks, and how the managers will make their employees do so in a comprehensive way.  Method: In order to carry out relevant information for this thesis, an abductive single case study was used. The qualitative data was conducted through semi-structured interviews with finance professionals at Volvo Group to retrieve appropriate data and valid information.  Findings: What was found from this research is a lack of the helicopter view among finance professionals today, which becomes the main barrier when they adapt to more qualitative work tasks. There are several steps which can be acknowledged from the two models McKinsey 7S and ADKAR in order to make the employees attain the helicopter view. Summarising these steps, it is clear that the main purpose for a successful adaptation process is to keep the employees involved and updated.

Automatisering med robotar blir mer relevant och förväntas öka framgent. Befintlig forskning på området fokuserar på privata organisationer och effekten av automatiseringen. Vidare finns det forskning kring hur beslut att implementera nya teknologiska innovationer enligt Technology-Organization-Environment-modellen (TOE-modellen), vilken används för att förklara hur en organisations kontext påverkar införandet av innovationer utifrån teknologi-, organisation samt miljökontext. Dock finns endast begränsat material att finna där modellen sätts i relation till RPA som innovation i offentlig sektor. Studien syftade till att undersöka hur faktorer enligt en utvecklad TOE-modell påverkar beslut att införa RPA som innovation i offentliga verksamheter. Genom semi-strukturerade intervjuer erhölls resultat som visade på att teknologi-, organisation- och miljökontext enligt TOEmodellen har en betydelse när beslut att införa RPA som innovation i verksamheten och således har TOE-modellen relevans även i offentliga verksamheter. Dock visade även resultatet på en viss skillnad kring ett fåtal av faktorerna, något som kunde härledas till skillnader i konkurrensförutsättningar mellan privat och offentlig sektor.