Thesis (M.S.)--North Carolina State University, 2003. / Title from PDF title page (viewed on Jan. 13, 2005). Includes vita. Includes bibliographical references (p. 76-86).

Population structure can be defined as a difference in the frequencies of alleles among populations, and it is often caused by barriers to dispersal. I used mitochondrial DNA sequences to examine the population structure of two large, aquatic salamanders, Amphiuma means (the Two-Toed Amphiuma) and Siren lacertina (the Greater Siren), at two spatial scales in peninsular Florida. Both species appear to have similar life histories, including poor dry land dispersal capabilities, and I hypothesized that they would exhibit significant population structure at both broad and fine scales. Wetland loss and landscape fragmentation are certain to have caused decreases in abundance and even extirpation of local populations of both species. Understanding current levels of genetic diversity is important in conservation efforts, particularly if individuals must be translocated in the future. A portion of the cytochrome b gene was analyzed for 34 Amphiuma means and 21 Siren lacertina sequences. A neighbor-joining tree and haplotype network showed that A. means exhibited statistically significant population structure at the broad, but not the fine scale. The lack of structure at the fine scale was attributed to periodic flooding among drainage basins in the low-lying study area. The same analyses showed that S. lacertina did not exhibit statistically significant population structure at the broad scale (analyses were not conducted at the fine scale because of low sample size). The difference in broad-scale population structure between the two species is the result of a difference in gene flow, which in turn, can be the result of a difference in effective population size (Ne), female dispersal capability and/or behavior, chance of bait-bucket transfer, or biogeographic history. The best of these possible explanations seems to be a difference in biogeographic history. For the two sites from which both species were sampled, estimated population divergence date was 3.3-6.4 million years ago (mya) for A. means and 0.04-0.5 mya for S. lacertina. The large difference in estimated divergence dates suggests that S. lacertina colonized peninsular Florida from somewhere else following a glacial sea level rise or an interglacial sea level fall, at a time when A. means was already present in refugia within peninsular Florida. This is the first study to explicitly examine the population genetics of A. means and S. lacertina, and future studies should examine their population genetics range wide, to elucidate how gene flow and/or historical biogeography may have contributed to population structure elsewhere.

The salamander family Sirenidae is composed of two extant genera, Siren and Pseudobranchus, each with two known species. Several questions regarding Sirenidae life history persist, and much of the available life history data for Pseudobranchus are attributed to studies of P. axanthus in northern Florida. Information on the reproductive biology of P. axanthus is limited, and historical references often suggest contradictory results. This study was undertaken to clarify information and expand on the limited data regarding P. axanthus reproductive biology, specifically for southern Florida populations. The study population was most likely the P. a. belli subspecies. P. axanthus in southern Florida exhibited year round, continuous reproduction with oviposition documented in nine months. Potential clutch size was positively correlated to female size. The largest observed potential clutch was a female with 58 pre-ovulatory oocytes. The largest observed bout was a female with 15 oviductal eggs. Female P. axanthus in southern Florida oviposited eggs singly and at total densities of 3-4 eggs/m2. Developmental time to egg hatching lasted about 30 days, larvae hatched at 16mm TL, and minimum size at female sexual maturity was 115mm TL and 72mm SVL. The reproductive biology of P. axanthus was distinctly contrasting to other members of the family Sirenidae, S. intermedia and S. lacertina, that occupy the same habitat at the same study location.

Amphibian decline is a major concern worldwide, and a lack of basic ecological and life history information for many species significantly limits our ability to evaluate the degree and possible causes of such declines, and to develop effective conservation strategies for threatened and endangered species. Not only is there a shortage of adequate long-term datasets necessary for robust analyses of population variability, but the elusive nature and obscure microhabitats of many species make it difficult to collect even the most basic natural history data. In a series of observational and experimental studies, I employed both traditional and novel ecological methodologies to examine environmental correlates of temporal population variability, foraging ecology and anti-predator behavior in endangered Barton Springs Salamander (Eurycea sosorum) from Austin (Travis County), Texas. Though headwater springs are typically thought of as habitats with relatively stable environmental conditions, I discovered that E. sosorum population abundance was strongly influenced by periodic extremes of rainfall that affect cycles in spring flow rates, water temperature, and other physico-chemical variables. I also found that population dynamics in E. sosorum are highly consistent with those expected for organisms with a storage effect life-history strategy, in which a few long-lived females capable of high fecundity and prolonged survival in subterranean habitat during adverse environmental conditions may be sufficient for population persistence. In addition, juveniles may use subterranean habitat as a thermal refuge. Using stable isotope analyses and macroinvertebrate prey censuses, I determined that at the population level, adult E. sosorum exhibits high electivity for planarian flatworms (Dugesia sp.). This would not have been detectable using traditional methods of dietary analysis such as stomach or fecal content analysis because Dugesia are soft-bodied animals. Additionally, stable isotope analyses revealed that adult E. sosorum exhibits inter-individual diet variation and is capable of diet switching. Finally, I discovered that anti-predator behavior in E. sosorum is influenced more strongly by visual and bioelectric cues from potential predators, but not olfactory cues. This is the first known demonstration of anti-predatory response mediated only by bioelectric stimuli in an amphibian, and one of very few to observe this phenomenon among aquatic vertebrates. / text

China harbors 10% of the world's salamander species. Studying their evolutionary history provides critical insights into the evolution of the fauna of the Far East. The stout newts (Pachytriton, also known as paddle-tailed newts) are a genus of aquatic montane salamanders that are widely distributed in southeastern China. Despite their longstanding popularity among the global pet trade, little is known of their biology beyond external morphology. My thesis presents the first systematic study to elucidate phylogenetic relationships, character evolution, biogeographic patterns, species delimitation, and speciation mechanisms in this genus.

The Jollyville Plateau Salamander (JPS), Eurycea tonkawae, is a species of salamander endemic to Texas, the United States. It is a candidate for protection under the Endangered Species Act. This report assesses the JPS population abundances at Lanier Spring, Long Hollow Creek at Wheless Spring, and Ribelin Spring in Austin using a mark-recapture method. The maximum likelihood estimation method was used to obtain the population size estimates under two models, the M₀ model and the M[subscript t] model. The M₀ model assumes that every animal has the same capture probability in the population for each sampling period while the M[subscript t] model allows capture probabilities to vary by time. Simulations were performed by using an MCMC algorithm based on the M₀ model. Between 2007 and 2009, the population size estimates for JPS (>16mm snout-vent length, (SVL)) at Lanier Spring varied between 86 and 554 under the M₀ model, between 80 and 549 under the M[subscript t] model, and between 76 and 564 using MCMC simulations. During 2007 monitoring periods, the population size estimates for JPS (>16mm SVL) at Ribelin Spring varied between 105 and 236 under the M₀ model, between 104 and 196 under the M[subscript t] model, and between 105 and 265 using MCMC simulations. During 2007 and 2008 monitoring periods, the population size estimates for JPS (>16mm SVL) at Wheless Spring varied between 368 and 1087 under the M₀ model, between 339 and 1075 under the M[subscript t] model, and between 411 and 1098 using MCMC simulations. Different estimation methods yielded consistent estimates. No clear population trends were detected due to the big fluctuations in estimates in this study. / text

The Coeur d’Alene salamander (Plethodon idahoensis) is a species of special concern throughout its global range, which includes southeastern BC (COSEWIC, Nov 2007), Idaho, and Montana, due to disjunct populations and sensitivity to human disturbance. Within the Interior Cedar-Hemlock forest on Mount Revelstoke, BC, Coeur d’Alene salamanders occur at their highest abundance between 600 m and 800 m. Beyond the Mount Revelstoke National Park boundaries, the low-order stream habitat of this species is subject to disturbance from forestry, mining, road building, road maintenance, and watercourse diversions such as run-of-the-river hydro projects. We conducted nocturnal salamander surveys and assessed habitat characteristics on 12 Sites (750 m² – 1000 m² stream transects) along three streams from June through September 2006. Coeur d’Alene salamanders were detected from 600 m - 1000 m on seven of the 12 study sites. Relative abundance of Coeur d’Alene salamanders ranged from 0.005 ± 0.001 per m² to 0.025 ± 0.005 per m² on six sites below 950 m. Coeur d’Alene salamanders occurred at an average of 0.001 ± 0.001 per m² at 972 m, the only site above 950 m where we detected salamanders. Our capture-mark-recapture efforts of three surveys per month in June and August yielded a very low recapture rate (3.95 %). Coeur d’Alene salamanders are challenging to enumerate due to their vertical distribution within the soil and underlying geological material. Neonate, juvenile, and adult Coeur d’Alene salamanders were observed from June to September and the highest proportion of neonates occurred in June, soon after the salamanders emerged from winter hibernation. Results of a logistic regression analysis of 1-m² plots reflected the importance of fine scale habitat characteristics (quadrat gradient, boulder, cobble, moss, grass, and shrub) in addition to site-level habitat features (water volume and elevation) that in combination describe the association of Coeur d’Alene salamanders with cool and moist conditions. Coeur d’Alene salamanders appear to select streambed habitat during warm, dry periods, which may be a behavioural response to minimize dehydration during periods of activity at the surface of the forest floor.

No description available.

The axolotl (Ambystoma mexicanum) has been used as a model organism for studying development, genetics, and regeneration. Although the sensory hair cells of the lateral line of this species have been shown to be able to regenerate, it is not known whether this also occurs in the inner ear. In fact, little is known about the hearing capabilities of the axolotl or other salamander species. I recorded auditory evoked potentials (AEPs) of six axolotls at eleven frequencies (0.1, 0.25, 0.4, 0.6, 0.8, 1, 1.5, 2, 3, 4, and 6 kHz) in order to produce baseline audiograms of underwater pressure sensitivity. Individuals were then subjected to a 48-hour, 150 Hz sound exposure at approximately 170 dB (re 1 μPa). AEPs were then performed to measure hearing thresholds immediately after sound exposure and at 2, 4, and 8 days post-sound exposure (DPSE). In the baseline audiogram, axolotls were most sensitive at 600 Hz, with an additional peak of sensitivity at 3 kHz. Following sound exposure, axolotls experienced a 6 to 12 dB temporary threshold shift (TTS) after sound exposure, with TTS being greatest at low frequencies near the 150 Hz stimulus frequency (i.e., 100 and 250 Hz). Hearing sensitivity returned to control levels within 8 DPSE. This indicates that axolotls do possess the ability to recover hearing sensitivity after damage following acoustical trauma. This study is the first to document hearing loss in the axolotl. Future studies are needed to correlate this hearing loss and recovery to sensory hair cell loss and regeneration in the axolotl inner ear.

Salamanders are critical components of forest ecosystems, in terms of total biomass, as well as for their value as indicators of ecosystem stress. Considering the worldwide decline in amphibian populations, the known effects of timber harvest on salamander populations, and the importance of the forest products industry in Kentucky and elsewhere, the impacts of silvicultural operations on salamander communities cannot be overlooked. The objective was to investigate the effects of three different silvicultural treatments, each involving different streamside management zone (SMZ) characteristics, on salamander communities in ephemeral streams. Data were collected by regular checks of pitfall traps, coverboards, and transect searches. Using both pre- and post-harvest data, abundance estimates were acquired using binomial mixture models. Declines in some species of terrestrial and stream-breeding salamanders were detected, and were shown to be likely related to characteristics of the corresponding silvicultural treatment. Applying modest SMZ regulations to ephemeral streams would likely alleviate these declines significantly.