The rate of global urbanization is increasing rapidly as the human population expands, leading to species loss and biotic homogenization. Less studied, however, is the effect of urbanization on the frequency and efficiency of species interactions. Animal-based seed dispersal interactions may be especially sensitive to urbanization because they depend on several factors: 1) the rate of seed dispersal interactions (feeding), 2) diversity of disperser species, 3) the probability of seed dispersal and 4) the probability of germination after seed dispersal. However, how urbanization disrupts species interactions, including seed-dispersal, is still poorly known. In this study, we evaluate differences in the frequency and efficiency of seed dispersal between urban and natural sites using Eastern Poison Ivy (Toxicodendron radicans) as the focal species. Individual T. radicans lianas within natural and urban sites were observed in twelve-minute intervals (total 185.8 hours) at urban and natural sites during which the number and identity of feeding avian species was recorded. A total of 9500 fruits between natural and urban sites were marked with a UV fluorescent dye. Undispersed marked fruits were recovered via seed traps to estimate probability of dispersal. Defecated fruits were collected from natural and urban sites to evaluate germination efficiency after dispersal. Feeding rate was twice as high in urban compared to natural sites (P=0.007). Additionally, seed disperser diversity was on average twice as high in urban sites and species composition was significantly different between natural and urban sites. However, probability of seed dispersal was not significantly different between urban and natural sites (P=0.3). Interestingly, germination rate was 20% higher in defecated seeds collected from natural sites compared to defecated seeds from urban sites (P=0.005). Our results suggest that while T. radicans attracts a higher number and greater diversity of seed dispersers in urban areas, overall dispersal success is the same or even greater at natural sites, as seeds have a higher chance of germinating after being consumed by dispersers at natural compared to urban sites. Species composition differences between sites may play an important role in germinability of seeds; differences in species’ feeding strategies result in differences in their ability to scarify seeds in their digestive systems, a necessary step for seeds that rely on animal seed dispersers. Urbanization can thus negatively affect seed dispersal interactions by altering the composition of disperser species. Other animal-based interactions may be similarly affected by urbanization, and thus we emphasize the need for further studies.

Symbiotic interactions among various organisms are often necessary for one or both individual's survival. These symbiotic relationships must be considered in restoration projects to allow for the successful establishment of the species. Rhizobia are nitrogen-fixing bacteria found in symbiotic relations with legumes. By utilizing this relationship, restoration practitioners can establish native legume species more successfully while repopulating soil microorganisms into degraded soils. Despite the potential benefits a rhizobium inoculant can have on restoration efforts, minimal research has been done to understand the impacts this treatment has on specific species and the systems they are employed within. Our research goal was to assess the efficacy of applying a commercial rhizobium product (EXCEED ®) and indigenous rhizobium strains on two lupine species (Lupinus argenteus Pursh and Lupinus sericeus Pursh), commonly used for rangeland seedings in the Great Basin region of the western United States. We conducted laboratory and field trials to meet this research goal, with the results of the laboratory experiments shared in chapter 1 and findings from the field reported in chapter 2. In chapter 1, we evaluated in the laboratory whether indigenous rhizobia strains could be isolated, cultured, and applied as a liquid inoculant or a seed coating to induce root nodulation and increase plant growth. The performance of these inoculums was compared against the commercial rhizobium product. Additionally, we tested in a trial if compost could be applied within the seed coating to improve the efficacy of the rhizobium treatment. Our research demonstrated that the commercial inoculum induced root nodulation, and in one of three trials, this treatment improved plant growth. We also found indigenous strains effectively formed nodules on the plant roots when applied through a liquid culture or a seed coating. However, the number of root nodules and the presence of a pink color (indicating nitrogen fixation) were typically higher in the commercial product than in the indigenous strains when applied through a seed coating. These short-term laboratory studies generally provided minimal evidence that rhizobia impacted plant growth. However, data indicated that having compost in the coating alone improved shoot biomass by 33% (P = 0.025). In chapter 2, research assessed the performance of the same rhizobia inoculums tested in the laboratory trials on a mine in northern Utah at two waste-rock sites, one comprised of crushed waste rock and the other made of waste rock amended with topsoil. One year after seeding, we had high plant recruitment at both study sites, and there were more plants, which were more vigorous, in the amended site (P<0.001). These results demonstrate that reclamation efforts on mineland overburden can be improved when topsoil is incorporated into the growing medium. At this stage in the study, there was no difference in plant establishment and vigor between any seed treatments, but future research is planned to assess these metrics in the next growing season. The lack of improvement in plant growth from a rhizobia treatment in some of our laboratory and field trials may be due to the short period of these studies. Nodules that form on mature root systems provide more nitrogen-fixing benefits than those formed on immature roots. Hence, future research should consider conducting trials for more extended periods to understand how the treatments influence the growth of mature plants. Because we found in the laboratory that the rhizobia inoculums were successful in nodulating the test species, we anticipate that future studies will find that these treatments can improve plant performance and subsequently restoration success.

Poor emergence of sorghum [(Sorghum bicolor (L) Moench] affects the stand and potential yields. The major objective of this research was to find correlations among field emergence data and laboratory seed vigor tests. Thirty-two and 30 hybrids of sorghum were planted at three Virginia locations in 1995 and 1996, respectively. Field emergence was subsequently compared with results from laboratory tests that included: 1) standard germination; 2) osmotic-stress using polyethylene glycol 8000 (mw); 3) heat-shock using 2 hr at 50°C stress; 4) electrical conductivity of steep water of germinating seeds; and 5) seed mass. Field emergence of grain sorghum differed among hybrids, years and locations. Mean emergence across years and locations was 67.5%, whereas mean germination in the laboratory was 87.8%. There were interaction between hybrid and location and between hybrid and year. Germination under optimal conditions (standard germination test) and with osmotic or heat-shock stress differed among hybrids. Regression analyses showed a weak correlation between laboratory germination (with or without stress) and field emergence in both years. The fresh weight of seedlings whether from standard germination or stress tests also differed among hybrids in both years, and the associations with field emergence were weakly correlated in 1996. Hybrids showed significant differences in radicle length when grown under laboratory stress in both years following standard germination. There was a weak correlation with field emergence and radicle length following heat-shock treatment in 1996. The measurement of electrical conductivity in the seed steep water showed significant differences among hybrids. A weak correlation with field emergence was seen in 1996. Conductivity values per gram of seed and per cm² of seed area revealed differences among hybrids. The correlation of these parameters with field emergence was higher than conductivity per seed. Seed mass varied among hybrids in both years , but was no correlation between seed mass and emergence. Of the laboratory parameters examined, germination proved to be the most consistent predictor of variations in field emergence of sorghum hybrids. / Ph. D.

Yes / The use of biological macromolecules like quince seed mucilage (QSM), as the common curative practice has a long history in traditional folk medicine to cure wounds and burns. However, this gel cannot be applied on exudative wounds because of the high water content and non-absorption of infection of open wounds. It also limits cell-to-cell interactions and leads to the slow wound healing process. In this study to overcome these problems, a novel QSM-based hybrid scaffold modified by PCL/PEG copolymer was designed and characterized. The properties of this scaffold (PCL/QSM/PEG) were also compared with four scaffolds of PCL/PEG, PCL/Chitosan/PEG, chitosan, and QSM, to assess the role of QSM and the combined effect of polymers in improving the function of skin tissue-engineered scaffolds. It was found, the physicochemical properties play a crucial role in regulating cell behaviors so that, PCL/QSM/PEG as a smart/stimuli-responsive bio-matrix promotes not only human-adipose stem cells (h-ASCs) adhesion but also supports fibroblasts growth, via providing a porous-network. PCL/QSM/PEG could also induce keratinocytes at a desirable level for wound healing, by increasing the mechanobiological signals. Immunocytochemistry analysis confirmed keratinocytes differentiation pattern and their normal phenotype on PCL/QSM/PEG. Our study demonstrates, QSM as a differentiation/growth-promoting biological factor can be a proper candidate for design of wound dressings and skin tissue-engineered substrates containing cell.

With herbicide efficacy declining as weeds continue to evolve and resist key modes of action, long-term, multi-faceted control practices need to be investigated. Two studies, involving cover crops and tillage, were implemented to understand how management practices influence the weed seed bank. The objectives of both studies were to examine long-term changes in the weed seed bank in response to tillage methods, fertility, and cover crops, and to analyze differences in distribution and community composition between individual species in the weed seed bank. To assess the weed community present in both seed banks, soil samples were collected, and a soil grow out was conducted in the greenhouse. To examine the field-emerged seed banks, percent coverage was collected for each weed species, crop residue, bare ground, and cover crop if present. The first study was established in 1970, evaluating four tillage systems: moldboard plow (CT), chisel plow (RT), alternative (AT), and no-tillage (NT). From 1970 to 1990, this study was continuous corn (Zea mays L.), but in 1991, soybeans (Glycine max L.) were added into the rotation, marking the beginning of the current corn-soybean rotation. Fertilizer treatments (no-fertilizer, nitrogen only, and NPK) were also evaluated. Each tillage and fertility treatment were replicated four times in the field in 6 m by 8 m plots. Weed seeds were found to be distributed within the soil profile differently by tillage treatment. No-till treatments maintained most of the seed bank near the surface of the soil. Based on the response of individual species to fertility treatments, community shifts in seed bank composition were found. LAMAM, STEME, and SIBVI had the greatest richness in NPK treatments compared to no fertilizer and nitrogen only. CERVU tended to favor treatments without any fertilizer. Tillage and fertility were also found to interact and influence species presence and community composition. The second study was established in 2013, to examine changes and differences in distribution and composition between individual species in the weed community in response to cover crop rotations and tillage. A split-plot design with three crop rotation systems was implemented: 1) corn (Zea mays L.) – cereal rye (Secale cereale L.) – soybean (Glycine max (L.) Merr.) – hairy vetch (Vicia villosa Roth) [CcrShv], 2) corn-cereal rye-soybean-oats + radish (Avena sativa L. + Raphanus sativus L.) [CcrSor], and 3) corn-no cover crop-soybean-no cover crop [NOCC], and two tillage treatments: conventional tillage and no-till. This field study also supported previous findings of higher weed diversity in no-till systems. ANOVA performed in R suggested species richness was significantly higher in no-tillage treatments in comparison to tillage treatments. For the field-emerged weed community, a pairwise comparisons test suggested cover crop treatments have significantly lower weed richness compared to plots with no cover crop present, but there was no interactive effect of tillage. 3-Way ANOVAs suggested time, tillage, and crop rotation influenced each weed species differently. Due to individual weed species having different requirements for germination and seed longevity, these data suggest the importance of developing and implementing a quality, integrated weed management program to maintain low levels of weed emergence and seed credits to the seed bank.

<p>Over the last two decades, mycoflorostic studies undertaken in Guyanese forests have uncovered hundreds of new fungal species and genera. One of the recovered fungal families was the Xylariaceae, although most were not identified to species. Members of this family play ecological roles as decomposers, endophytes, and pathogens of vascular plants and grass species. In addition, this group is increasingly recognized as a significant source of novel metabolic products with potential for applications in medicine, agriculture, and industrial biofuel. Given its potential, we took a more targeted approach to the documentation of this group. Our sampling efforts drawing on more than two decades of field collections yielded ca. 90 species in 12 genera, including a putative pathogen, <em>Xylaria karyophthora</em> of the seeds of <em>Chlorocardium</em> spp (Greenheart). Despite the significance of Greenheart to the Guyanese economy, little is known about the biology and ecology of this fungus. Due to the lack of available resources to study this fungus, our objectives were two-fold: first, to sequence and annotate the genome of <em>X. karyophthora</em> to provide a resource for genome-centric explorations, and to use this genome to infer the biology and ecology of this fungus. We focused on identifying and characterizing secretomes, viz. carbohydrate-active enzymes (CAZymes) and secondary metabolites biosynthetic gene clusters (SMBGCs) to infer the nutritional strategy of this fungus. Our results suggest that <em>X. karyophthora</em> has the capacity to act as both an endophyte and a pathogen. To make further inferences about the population, we used SSR markers to elucidate the genetic diversity and population structure of <em>X. karyophthora</em>. <em>X. karyophthora</em> populations have high genetic diversity, potentially exploiting both outcrossing and inbreeding reproductive strategies, and demonstrate a pattern consistent with human-mediated spread. This work will contribute information on new species and records of Xylariaceous fungi and their allies from Guyana with particular emphasis on unraveling the epidemiology, genetic diversity, and population structure of <em>X. karyophthora.</em></p>

In western North America, winterfat (Krascheninnikovia lanata (Pursh) A. Meeuse & Smit) is a valuable protein-rich subshrub whose restoration has been limited by poor seed flowability and low rates of seedling establishment. Seed flowability can be limited by a dense covering of hairs on winterfat fruits that can cause them to clog in mechanized equipment. Seedling establishment can be limited by premature germination of fall-sown seeds that can cause over-winter seedling mortality from freezing, pathogen attack, and winter drought. Seed coatings may provide a way to overcome both of these barriers to winterfat restoration. Coatings can compress hairs against the fruit and improve seed flowability, and a hydrophobic polymer within seed coatings can repel water and delay germination of fall-sown seeds until spring, when winter hazards have subsided, and conditions are more conducive to seedling establishment. With the advent of this technology, there is a need to establish cultural practices, such as optimal planting depth, for coated winterfat fruits. In chapter 1 of this thesis, we evaluated the influence of planting depth, seed coatings, and their interactions on winterfat seedling emergence under laboratory and field conditions. We predicted that seedling emergence would be greatest from shallow planting depths, and that coatings would not affect emergence. Results generally supported our hypothesis, with seedling emergence being highest from surface-sown and shallow-planted seeds for both non-coated (control) and coated winterfat fruits in laboratory and field conditions. Emergence from surface-sown seeds was more than two-fold greater than from the deepest planting depth (12.7 mm). Seed coatings improved emergence of surface-sown seeds compared to the control by 52 – 168% in the laboratory but had no effect in the field. As predicted, emergence was similar between coated and non-coated fruits when sown below the soil surface in both laboratory and field conditions. These results suggest that seed coatings may improve winterfat restoration success by improving flowability without inhibiting emergence, allowing the species to be used in more seeding projects. Winterfat seed coatings may be improved with the use of a hydrophobic polymer to delay germination of fall-sown seeds until spring. In chapter 2 of this thesis, we compared seedling emergence from non-coated seeds, calcium carbonate coated seeds (blank-coated), and seeds coated with calcium carbonate plus an exterior hydrophobic coating. We counted the number of live seedlings and those that had died after emerging, and calculated mortality percentages for each treatment. We hypothesized that emergence would be greatest from hydrophobic-coated seeds, and the results supported our hypothesis. Seedling emergence from hydrophobic-coated seeds was three-fold greater than the control, and five-fold greater than blank-coated seeds. Mortality percentages were highest for the control, lower for blank-coated seeds, and lowest for hydrophobic-coated seeds. Thus, hydrophobic seed coatings can improve winterfat seedling emergence, and so could be instrumental restoring this valuable species to degraded rangelands.

Two studies were conducted from 2017 – 2019 at Stoneville, MS the first was to determine optimum seeding rates on single and twin row configuration during both late and early planting dates. The second study was to compare two row configurations with two planting populations (whole plot) but add management strategies (subplots) within each whole plot. From this data, yield optimization could be reached within each system that likely encompasses everything a producer in the Mississippi Delta could encounter during a growing season. Soybean seed from this data was analyzed for protein, oil, and fatty acid composition to determine which systems produce greater seed quality in terms of seed composition. Normal (non-symptomatic) and seed visually infected with purple seed stain (PSS) were compared to determine how infected soybean seed compared to normal soybean seed. Both seed (infected and non-symptomatic) were compared by measuring seed composition, germination, and vigor. During 2018 and 2019 at Verona, MS the same whole plot with subplot test was used but at this location under rained conditions. Soybean grown under rained conditions is a common practice in that particular region of the state (Northeast) so treatments were designed to be useful to producers in that area.

Waste rice in harvested fields and natural seeds in moist-soil wetlands are important foods for waterfowl in the Mississippi Alluvial Valley (MAV). Waste rice declines significantly between harvest and late autumn. I conducted experiments in 19 rice fields in Arkansas and Mississippi during autumns 2003 and 2004 to evaluate the ability of common post-harvest practices to conserve waste rice between harvest and late autumn. Greatest mean abundance of waste rice in late autumn occurred in standing stubble (x&95 = 105 kg/ha; CL = 72.84, 150.16 kg/ha) followed by burned (x&95 = 72 kg/ha; 49.57, 105.81 kg/ha), mowed (x&95 = 67 kg/ha; 46.65, 97.42 kg/ha), rolled (x&95 = 51 kg/ha; 35.54, 73.076 kg/ha), and disked stubble (x&95 = 48 kg/ha; 33.26, 68.41 kg/ha). I recommend leaving stubble or burning fields to create interspersion of stubble and water after flooding. Additionally, I estimated abundance of moist-soil seed in 72 management units on public lands throughout the MAV for autumns 2002-2004. Mean seed abundance was 496 kg/ha (SE = 62). I recommend increased active management of moist-soil wetlands to mitigate decreased availability of waste rice in the MAV.

No description available.