A produção de alimentos orgânicos amplia a demanda por sementes e a atual legislação brasileira requer a utilização de sementes orgânicas. O tratamento das sementes com produtos biológicos, à base de Bacillus e Trichoderma, possibilita o controle de patógenos e interfere na germinação da semente e no desenvolvimento da plântula. Deste modo, foi avaliado o parâmetro fisiológico das sementes de cenoura (Daucus carota L.) após aplicação de produtos biológicos à base de Bacillus spp. e Trichoderma spp., com avaliações imediatas e aos 60, 120 e 180 dias de armazenamento. Para isto, foram utilizadas sementes de cenoura, quatro cultivares de verão, \'Brasília\', \'Bruna\', \'Erica\' e \'Francine\', considerando os seguintes tratamentos: Controle, Bacillus spp. cepas 1 e 2, Trichoderma spp. e Trichoderma harzianum e avaliadas por meio dos testes de germinação, emergência da plântula, primeira contagem de germinação, índice de velocidade de germinação, índice de velocidade de emergência da plântula, comprimento da raiz e da plântula e sanidade. O Trichoderma harzianum causa fitotoxidez nas plântulas de cenoura dos cultivares Brasília, Bruna e Erica, quando avaliadas, no teste de germinação e quanto ao comprimento, imediatamente após a aplicação dos produtos nas sementes. Em termos fisiológicos, a qualidade das sementes dos cultivares Brasília, Bruna e Erica, não foi afetada quando tratadas previamente ao armazenamento. A eficácia do controle dos principais patógenos das sementes de cenoura variou em função do armazenamento das sementes tratadas, bem como do cultivar. Os produtos biológicos reduziram a incidência dos principais patógenos das sementes de cenoura. / Organic foods production increases the demand for seeds and current Brazilian legislation requires the use of organic seeds. The treatment of seeds with biological products, based on Bacillus and Trichoderma, allows the control of pathogens and interferes in seed germination and seedling development. The physiological parameters of carrot seeds (Daucus carota L.) were evaluated immediately after the treatment with biological products based on Bacillus spp. and Trichoderma spp., and upon 60, 120 and 180 days of storage. Four summer cultivars of carrot seeds \'Brasília\', \'Bruna\', \'Erica\' and \'Francine\', were analyzed considering the following treatments: Control, Bacillus spp. Strains 1 and 2, Trichoderma spp. and Trichoderma harzianum, and they were evaluated by germination tests, seedling emergence, first germination count, germination speed index, seedling emergence speed index, root and seedling length, and sanity. Trichoderma harzianum causes phytotoxicity in the carrot seedlings of the cultivars Brasília, Bruna and Erica, when evaluated the germination test and in the length, immediately after the application of the products in the seeds. In physiological terms, the seed quality of the cultivars Brasília, Bruna and Erica, were not affected when treated prior to storage. The effectiveness of the control to the main pathogens of the carrot seeds varied as a function of the treated seeds storage, as well as of the cultivar. Biological products have reduced the incidence of major pathogens in carrot seeds.

Although work on the prolamins of pearl millet (Pennisetum glaucum) has revealed partial amino acid sequences for several alcohol-soluble storage proteins (Marcellino et al. 2002) the genes encoding them have not yet been isolated. We constructed a cDNA library from developing P. glaucum seed tissue and screened it using maize zein gene probes to isolate several α-prolamin-like gene sequences. The proteins encoded by these genes generally fall into two size classes: 20.6kD and 27.1kD, which we call the 21kD and 27kD pennisetins. Both proteins are similar in composition and sequence to α-prolamins from maize, sorghum and Coix. Protein bodies that appear as occlusions within the rough ER of P. glaucum endosperm cells are also very similar in size and shape to maize and sorghum protein bodies. The SDS-PAGE gel of the alcohol soluble protein fraction shows two distinct bands in the region corresponding to the 19kD and 22kD of maize α-zein. Both classes of pennisetins appear to be more similar to the 19kD α-zein of maize than to the 22-kD α-zein judging from sequence homology and maize antibody binding. Phylogenetic reconstruction suggests that P. glaucum may have branched from maize prior to the gene duplication which created the 19kD and 22kD α-zein families.

Lectins and hemagglutinins are carbohydrate binding proteins present in a diversity of organisms including humans, vertebrate and invertebrate animals, plants, fungi, and bacteria. They are usually the abundant storage proteins in leguminous plants. They display a host of biological activities such as antitumor, antifungal, antiviral, insecticidal, and antibacterial activities. / The biological properties of isolated proteins, including hemagglutinating, antifungal, anti-tumor and HIV-1 reverse transcriptase inhibitory activities, were examined. Their biochemical and biological properties were compared with other purified proteins. / The seeds contain an abundance of proteins, some of which are storage proteins but may play a role of protection from pathogenic microbes and phytophagous insects. Antifungal peptides/proteins, antiviral proteins, ribosome-inactivating proteins, proteinase inhibitors, chitinases, proteinases, and defensins, are some examples of the myriad of seed proteins. The aforementioned proteins are collectively called plant defense proteins in view of their antipathogenic activities. These antifungal proteins exhibit a wide range of molecular masses and amino acid sequences. / Two lectins with potentially exploitable activities were purified from Capparis spinosa seeds and Hibiscus mutabilis seeds, respectively. A hemagglutinin was isolated from Phaselous vulgaris , cultivar "French bean 35", and detailed apoptotic pathway in breast cancer cells, MCF-7 cells, was investigated. A novel dimeric beta-lactoglobulin-like antifungal protein and an antifungal amidase were purified from Passiflora edilus seeds and Peltophorum pterocarpum, respectively. / Lam, Sze Kwan. / Adviser: Tsi Bun Ng. / Source: Dissertation Abstracts International, Volume: 72-04, Section: B, page: . / Thesis (Ph.D.)--Chinese University of Hong Kong, 2010. / Includes bibliographical references (leaves 188-204). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Electronic reproduction. Ann Arbor, MI : ProQuest Information and Learning Company, [200-] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstract also in Chinese.

The structure of 11S cruciferin has been solved; however, how the individual subunits contribute to its physico-chemical and functional properties are not well known. The cruciferin isoforms in Arabidopsis thaliana, CRUA, CRUB, and CRUC, were investigated with respect to their molecular structures and the relationship of structural features to the physico-chemical and functional properties of cruciferin using homology modeling and various analytical techniques. Comparison of these models revealed that hydrophobicity and electrostatic potential distribution on the surface of the CRUC homotrimer had more favorable interfacial, solubility, and thermal properties than those of CRUA or CRUB. Flavor binding and pepsin digestion were associated with hypervariable regions (HVRs) and center core regions, respectively, moreso for CRUA and CRUB homotrimers than for CRUC. Chemical imaging of a single cell area in wild type (WT) and double-knockout seeds (CRUAbc, CRUaBc, and CRUabC) using synchrotron FT-IR microscopy (amide I band, 1650 cm-1, νC=O) showed that seed storage proteins were concentrated in the cell center and protein storage vacuoles, whereas lipids were closer to the cell wall. Secondary structure components of proteins of double-knockout lines did not show major differences. Changes in protein secondary structure components of pepsin-treated CRUabC (CRUC) mutant were minimal, indicating low enzyme accessibility. A three-step chromatographic procedure allowed isolation of the hexameric form of cruciferin with high purity (>95%). Fourier transform infrared (FT-IR) and circular dichroism (CD) spectroscopic analysis of the secondary structure of these proteins revealed cruciferins were folded into higher order secondary structures; 44−50% β-sheets and 7−9% α-helices. The relative subunit ratio was approximately 1:3:6 (CRUA:CRUB:CRUC) in the WT cruciferin. The Tm values of purified cruciferin at pH 7.4 (μ = 0.0) were in the order of WT = CRUA = CRUB < CRUC. The order of surface hydrophobicity as determined by ANS (1-anilinonaphthalene-8-sulfonate) probe binding was CRUA > CRUB = WT >> CRUC. Intrinsic fluorescence studies revealed a compact molecular structure for the CRUC homohexamer compared to the CRUA and CRUB homohexamers. The order of emulsion forming abilities was CRUA = CRUB > WT > CRUC (no emulsion formation) and the order of heat-induced network structure strength was WT > CRUA = CRUB > CRUC (no gel formation). The inability of CRUC to form gels or emulsions may be attributed to its low surface hydrophobicity and molecular compactness. At pH 2.0, CRUC hexamers dissociated into trimers which allowed the formation of an O/W emulsion and heat-induced network structures. Solubility of cruciferin as a function of pH at low ionic strength gave two minima around pH 4 and 7.4 yielding a “W” shape solubility profile deviating from the typical “U” or “V” shape solubility profile of other 11S globulins. The high ionic strength (μ = 0.5) was not favorable for emulsification, heat-induced gel formation, or solubilization for all cruciferins. Furthermore, the CRUA and CRUB homohexamers exhibited rapid pepsinolysis, while the CRUC homohexamer and WT heterohexamer were digested more slowly. Although fairly well conserved regions were found in the primary structure of these three cruciferin subunits, differences were found in the hypervariable regions and extended loop regions resulting in slight differences in 3D structures and interactions that occur during association to form superstructures, such as hexamers. These differences were reflected in the physico-chemical and techno-functional properties of hexamers and trimers composed of each subunit. In silico predictions for certain functionalities were highly correlated with empirical data from laboratory experiments.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / The Balfourodendron riedelianum of the family Rutaceae is a native forest species with timber value and is used in the manufacture of luxury furniture, construction, and so forth. Moreover, the species is suitable for medical use, insecticide, landscaping and recovery riparian areas. Despite the importance of this species, there are few studies in relation to seed technology, irrigation in the production of seedlings and field early growth. In this sense, species diaspores were collected in Palma Nova (RS), in the years of 2013 and 2015, and further processed and divided into samples for testing. The study was divided into three articles, in Article I – measurement of biometrics, pre-germination treatments and substrates for the germination of B. riedelianum diaspores; by using a sample of 2013 for physical characterization, through the weight of a thousand seeds (PMS), water content (TU) and biometrics, besides the germination test (G%) on different substrates after breaking dormancy. The dimensions of the B. riedelianum seeds had on average, 18.59; 9.03 and 9.38 mm in length; width and thickness, respectively. The dormancy was broken by immersion in cold water (8 ± 2 ° C) for 48 hours and germinating was conducted on vermiculite substrate. Article II – In the first, two experiments were carried out, the quality of the seeds collected in 2013, which have been stored for two years in cold and humid chamber was evaluated and sampled for TU testing, G% and sanity, and the second evaluated in the lot collected in 2015 the disinfestation of the seeds in the incidence of fungi and germination. Diaspores of B. riedelianum remained viable for 12 months under storage conditions, and disinfection with Orthocide® is indicated in order that provided high G% and IVG. The genus Aspergillus sp., Fusarium sp., Cladosporium sp. and Penicillium sp. were detected in both experiments. Article III - identified water depth required to optimize the growth of seedlings of B. riedelianum, and evaluated its initial field growth. Combinations were used of heads between 4, 8 and 12 mm day-1, after 180 days of water management application survival evaluation were performed, height, stem diameter, dry mass air, root dry weight and quality index Dickson. The treatments described in the vivarium were conducted in the field, wich 450 days after plantating were evaluated in relation to H, DC, MSA, leaf area and root length, chlorophyll content and chlorophyll a fluorescence. The head influenced the growth of seedlings of B. riedelianum, in the vivarium stage, which was confirmed in the field. Seedlings of Balfourodendron riedelianum can be produced initially with water depth of 4 mm day-1, which must be switched to 12 mm day -1after 60 days and remain so until the end of production. / Balfourodendron riedelianum da família Rutaceae é uma espécie florestal nativa com valor madeireiro, sendo utilizada na fabricação de móveis de luxo, construção civil, entre outros. Além disso, a espécie é indicada para uso medicinal, inseticida, paisagismo e recuperação de áreas ripárias. Apesar da importância desta espécie, há escassez de estudos em relação à tecnologia de sementes, à irrigação na produção de mudas e crescimento inicial a campo. Nesse sentido, foram coletados diásporos da espécie em Nova Palma (RS), em 2013 e 2015, após beneficiados e divididos em amostras para a realização dos testes. O estudo foi dividido em três capítulos, no Capítulo I - foi mensurado a biometria, tratamentos pré-germinativos e substratos para germinação de diásporos de B. riedelianum; sendo utilizado uma amostra do lote de 2013 para caracterização física, por meio do peso de mil sementes (PMS), teor de água (TU) e biometria, além do teste de germinação (G%) em diferentes substratos após superação da dormência. As dimensões dos diásporos de B. riedelianum apresentam em média, 18,59; 9,03 e 9,38 mm de comprimento; largura e espessura, respectivamente. A dormência foi superada pela imersão em água fria (8 ± 2 °C), por 48 horas e germinação pode ser conduzida em substrato vermiculita. No Capítulo II - foram realizados dois experimentos, no primeiro, foi avaliado a qualidade dos diásporos coletados em 2013 que foram armazenados durante dois anos em câmara fria e úmida, sendo retiradas amostras para testes de TU, G% e sanidade, no segundo foi avaliado no lote coletado em 2015 a desinfestação dos diásporos na incidência de fungos e na germinação. Os diásporos de B. riedelianum mantiveram-se viáveis durante 12 meses sob as condições de armazenamento, e a desinfestação com Orthocide® é indicado, tendo em vista, que proporcionou elevada G% e IVG. Os gêneros Aspergillus sp., Fusarium sp., Cladosporium sp. e Penicillium sp. foram detectados em ambos os experimentos. No Capítulo III – foi identificado a lâmina de irrigação necessária para otimizar o crescimento das mudas de B. riedelianum, e avaliado seu crescimento inicial a campo. Foram utilizados combinações entre as lâminas 4, 8 e 12 mm dia-1, que após 180 dias de aplicação do manejo hídrico realizou-se avaliações da sobrevivência (Sob), altura (H), diâmetro do coleto (DC), massa seca aérea (MSA), massa seca radicular (MSR) e índice de qualidade de Dickson (IQD). Os tratamentos descritos no experimento do viveiro foram conduzidos a campo, que aos 450 dias após o plantio foram avaliados em relação a H, DC, MSA, área foliar e comprimento radicular, teor de clorofila e fluorescência da clorofila a. As lâminas influenciaram no crescimento das mudas de B. riedelianum, na fase de viveiro, o que foi confirmado no campo. Mudas de Balfourodendron riedelianum podem ser produzidas, inicialmente, com lâmina de irrigação de 4 mm dia-1, a qual deve ser alternada para 12 mm dia-1 a partir dos 60 dias, permanecendo assim até o final da produção.

Resumo: O crambe (Crambe abyssinica Hochst) é uma planta da família Brassicaceae cujas sementes possuem elevado teor de óleo, sendo destinado no Brasil, principalmente, para a produção de biodiesel. É uma cultura que apresenta baixo custo de produção, seu cultivo é totalmente mecanizado com semedura no inverno e favorece a rotação com outras culturas de verão. No Brasil, a produção e a comercialização de sementes de crambe iniciaram-se há poucos anos, por isso estudos na área de tecnologia de produção são essenciais para obtenção de sementes de boa qualidade. Dentre os fatores que afetam a qualidade das sementes está o processo de secagem. A diminuição do teor de água das sementes em níveis adequados permite preservar as sementes por um período prolongado, possibilitando menor perda de vigor e germinação ao longo do armazenamento. O objetivo deste estudo foi avaliar o efeito de diferentes métodos de secagem sobre a qualidade de sementes de crambe no decorrer do armazenamento. O campo de produção de sementes de crambe foi instalado na Fazenda Experimental Lageado e os tratamentos experimentais foram conduzidos no Laboratório de Processamento de Produtos Agrícolas, pertencente ao Departamento de Engenharia Rural, e na Fazenda Experimental Lageado. As avaliações de qualidade foram realizadas no Laboratório de Análise de Sementes pertencente ao Departamento de Produção Vegetal - Setor Agricultura... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Crambe (Crambe abyssinica Hochst) is a member of the Brassicaceae family. Its seeds have high oil contents and its growth in Brazil aims to produce bio diesel. It is a low cost production and its cultivation is totally mechanized. It is also sowed at winter time, which favors crop rotation with other summer crops. In Brazil, Crambe seeds production and commercialization began a few years ago. The research in technology production is essential to obtain high quality seeds. Among the factors that affect seed quality there is the drying process. Seed water content decreased to an appropriate level allowing for a prolonged preservation time and enabling less vigor and germination loss during storage. The aim of this study was to evaluate the effect of different drying methods on Crambe seeds quality during storage. The seeds were produced in Fazenda Lageado, Faculdade de Ciências Agronômicas, UNESP, Botucatu / SP. Experimental treatments were conducted at Laboratório de Processamento de Produtos Agrícolas, Departamento de Engenharia Rural, and Fazenda Experimental Lageado. Evaluations on seed quality were conducted at Laboratório de Análise de Sementes, Departamento de Produção Vegetal - Setor Agricultura. Seeds were submitted to the following drying methods: a) seed drying in the shade with natural ventilation; b) artificial drying method using heated air; c) artificial drying method using unheated air; d) drying on ceramic patio; e) drying on the mother plant. Seeds were stored in laboratory conditions with bimonthly monitoring of their quality during a 8 month period. The following testes were performed: seed moisture content, standard germination test, first count of germination test, seedling emergence,emergence speed index and electrical conductivity. The accelerated ageing and seedling growth tests were performed on the 8th month of storage. The data obtained in each storage... (Complete abstract click electronic access below) / Orientador: Marco Antonio Martin Biaggioni / Coorientador: Cláudio Cavariani / Banca: Elza Alves / Banca: João Nakagawa / Mestre

Made available in DSpace on 2014-08-20T13:44:32Z (GMT). No. of bitstreams: 1 dissertacao_mario_flavio_magalhaes.pdf: 390698 bytes, checksum: b63a52591a132180ce56f1e7d631a56b (MD5) Previous issue date: 2012-12-18 / The products to be applied in seed treatment must provide sufficient protection to both seed and seedlings in the field, keeping the fungi, insects and nematodes properly controlled. The aim of this study was to evaluate the possible immediate and latent effect of the product Avicta Complete (fungicide, insecticide and nematicide), Cruiser 350 FS + Maxim Advanced and Maxim Advanced on the physiological quality of hybrid maize seed. We used seven hybrids from Monsanto: DKB 330, DKB 350, DKB 390, AG 7000, AG 8021, AG 8015 and AG 9010. Seeds were subjected to the following treatments 1) Control, 2) Avicta Complete®, 3) Cruiser 350 FS® + Maxim advanced®, 4) Maxim Advanced®, and then stored at room temperature for up to 360 days. The assessments were made at 45, 90, 180 and 360 days, and the attributes evaluated were: germination, accelerated aging, speed of emergence and cold test. The data was analyzed according to statistical analysis on the effect of isolated products and the interaction treatment/storage in which the following conclusions were obtained: 1) The treatment on the seeds varies according to the hybrid; 2) In general the phytotoxic effect of the seed treatment is detected after 90 days in ambient storage of the seeds; 3) The fungicide Maxim Advanced did not result in any phytotoxic effect in the hybrids during storage in a not climacteric environment and 4) Corn seed storage for 360 days at cold conditions and treated with Avicta completo and Cruiser plus Maxim Advanced, remain with high physiological quality. / Os produtos a serem aplicados no tratamento de sementes devem oferecer proteção suficiente tanto às sementes como às plântulas no campo, mantendo os fungos, insetos e nematóides devidamente controlados. O objetivo do presente trabalho foi verificar o possível efeito imediato e latente do produto Avicta Completo (ação fungicida, inseticida e nematicida), Cruiser 350 FS + Maxim Advanced e Maxim Advanced sobre a qualidade fisiológica de sementes de milho híbrido. Foram utilizados sete híbridos da empresa Monsanto: DKB 330, DKB 350, DKB 390, AG 7000, AG 8021, AG 8015 e AG 9010. As sementes foram submetidas aos seguintes tratamentos: 1) testemunha, 2) Avicta Completo ®, 3) Cruiser 350 FS® + Maxim Advanced®, 4) Maxim Advanced®, sendo então armazenadas em temperatura ambiente por até 360 dias. As avaliações foram feitas aos 45, 90, 180 e 360 dias, com as seguintes características avaliadas: germinação, envelhecimento acelerado, índice de velocidade de emergência e teste de frio. Os dados foram discutidos conforme análise estatística sobre o efeito isolado dos produtos e a interação tratamento/armazenamento no qual se obtiveram as seguintes conclusões: 1) o efeito do tratamento de sementes varia entre híbridos de milho em condições ambientais; 2) em geral, os efeitos fitotóxicos dos tratamentos em sementes manifestam-se a partir dos 90 dias de armazenamento, em condições ambientais; 3) O fungicida Maxim Advanced testado não apresentou efeito fitotoxico aos híbridos durante o armazenamento em ambiente não climatizado e 4) sementes de milho híbrido armazenadas por 360 dias em condições climatizadas e tratadas com Avicta completo e Cruiser mais Maxim Advanced, permanecem com alta qualidade fisiológica.

Made available in DSpace on 2014-08-20T13:44:34Z (GMT). No. of bitstreams: 1 dissertacao_adhemar_antonio_de_oliveira_junior.pdf: 235690 bytes, checksum: fdf540bfb4ba71eeef37d73bd6b35be8 (MD5) Previous issue date: 2012-12-18 / The products to be applied in the disinfestation of seed shall provide sufficient protection to seed as much as the seedlings in the field, keeping the fungi, insects and nematodes properly controlled. The objective of this study was to investigate the possible effect immediate and latent product Avicta Complete® Pack (with fungicide, insecticide and nematicide action) on the physiological quality of hybrid maize seed. Was used six hybrids from Pioneer Company: 30K73, 30F35, 30F80, 30K75, 30R50 and 32R22. The seeds were submitted to the following health treatments: 1) control, 2) Avicta Complete®, 3) overdose Avicta Complete® 100%, and then stored at room temperature and cold chamber (100 C and 60% RH) for 350 days. The evaluations were performed at 50, 150, 250 and 350 days, and the characteristics evaluated: germination, cold test and accelerated aging. Assays were performed at the Laboratory of Seeds of Tropical Breeding and Genetics Company and the data were subjected to statistical analysis using the statistical program Sisvar® (FERREIRA, 2000). Data were analyzed according to statistical analysis on the isolated effect of the products and environment interaction / storage in which we obtained the following conclusions: the reduction of the physiological quality of corn seeds varied depending on the treatment of the hybrid and the time that seeds remain stored after treatment and the reduction of physiological seed quality intensified with prolonged storage of the treated seed, it is validating therefore the secure processing and storage Avicta Complete® Pack with up to 250 days, which helps the management of treatment on seed processing units in Seed Corn Companies. / Os produtos a serem aplicados no tratamento sanitário de sementes devem oferecer proteção suficiente tanto às sementes como às plântulas no campo, mantendo os fungos, insetos e nematóides devidamente controlados. O objetivo do presente trabalho foi verificar o possível efeito imediato e latente do produto Avicta Completo (ação fungicida, inseticida e nematicida) sobre a qualidade fisiológica de sementes de milho híbrido. Foram utilizandos seis híbridos da empresa Pioneer: 30K73; 30F35; 30F80; 30K75; 30R50 e 32R22. As sementes foram submetidas aos seguintes tratamentos sanitários: 1) testemunha, 2) Avicta Completo®, 3) Superdosagem de Avicta Completo® 100%, sendo então armazenadas em temperatura ambiente e câmara fria(10oC e 60% de Umidade Relativa) por 350 dias. As avaliações foram feitas aos 50, 150, 250 e 350 dias, sendo as características avaliadas: germinação, teste de frio e envelhecimento acelerado. Os ensaios foram realizados no Laboratório de Sementes da empresa Tropical Melhoramento e Genética e os dados foram submetidos à análise estatística, utilizando-se o programa estatístico Sisvar® (FERREIRA, 2000). Os dados foram discutidos conforme análise estatística sobre o efeito isolado dos produtos e a interação ambiente/armazenamento no qual se obteve as seguintes conclusões: A redução da qualidade fisiológica de sementes de milho variou em função do tratamento, do híbrido e do tempo em que as sementes permaneceram armazenadas após o tratamento e a redução da qualidade fisiológica das sementes intensificou-se com prolongamento do armazenamento das sementes tratadas, validando, portanto, a segurança do tratamento e armazenamento com Avicta Completo até 250 dias, o que ajuda ao gerenciamento do tratamento nas unidades de beneficiamento de sementes das empresas produtoras de sementes de milho.

No description available.

As sementes de Myrciaria dubia apresentam curta longevidade e demandam a ampliação do conhecimento sobre fatores interferentes na sua conservação. Diante disso, o presente trabalho objetivou verificar as influências do grau de umidade e da temperatura do ambiente na manutenção da qualidade dessas sementes. Para tanto, sementes com 48, 43, 40, 34, 30, 24, 18 e 14% de água, acondicionadas em sacos de polietileno, foram armazenadas sob temperaturas controladas de 10ºC, 20ºC e 30ºC, durante 280 dias, e submetidas, periodicamente, a avaliações do grau de umidade, da germinação, do vigor e da sanidade. Foi constatado que as sementes têm a conservação favorecida pela associação do grau de umidade de 43% com a temperatura de armazenamento de 10°C; sob essa condição, a incidência de Fusarium sp. não prejudica o desempenho fisiológico das sementes. / Seeds of Myrciaria dubia have a short lifespan and increased knowledge of the factors that interfere with their conservation is required. The aim of this study was to verify influences of moisture content and storage temperature on the maintenance of seed quality. Seeds with 48, 43, 40, 34, 30, 24, 18 and 14% water were kept in polyethylene bags at 10ºC, 20ºC and 30ºC for 280 days, and submitted to periodic evaluations of moisture content, germination, vigour and sanity. Seed conservation was favored by the 43% water and 10°C storage temperature association. In this condition, the incidence of Fusarium sp. does not hamper the seed physiological performance.