Pythium diseases are common in the greenhouse production of tobacco transplants and can cause up to 70% seedling loss in hydroponic (float-bed) greenhouses. However, the symptoms and consequences of Pythium diseases are often variable among these greenhouses. A tobacco transplant greenhouse survey was conducted in 2017 in order to investigate the sources of this variability, especially the composition and distribution of Pythium communities within greenhouses. The survey revealed twelve Pythium species. Approximately 80% of the surveyed greenhouses harbored Pythium in at least one of four sites within the greenhouse, including the center walkway, weeds, but especially bay water and tobacco seedlings. Pythium dissotocum, followed by P. myriotylum, were the most common species. Pythium myriotylum, P. coloratum, and P. dissotocum were aggressive pathogens that suppressed seed germination and caused root rot, stunting, foliar chlorosis, and death of tobacco seedlings. Pythium aristosporum, P. porphyrae, P. torulosum, P. inflatum, P. irregulare, P. catenulatum, and a different isolate of P. dissotocum, were weak pathogens, causing root symptoms without affecting the upper part of tobacco seedlings. Pythium adhaerens, P. attrantheridium, and P. pectinolyticum did not affect tobacco seeds or seedlings. The consequences of Pythium infection were more likely to be severe when they occurred during seed germination than at seedling emergence, or after plant stem elongation when seedling roots had started to grow into underlying nutrient solutions, depending on the species of Pythium. High and low variation was observed among isolates of P. dissotocum and P. myriotylum, respectively. Pythium myriotylum co-existed with multiple other Pythium or oomycete species in the same environments within tobacco greenhouses, and significant in vitro and/or in vivo interactions between P. myriotylum and some naturally co-existing species were revealed. Pythium porphyrae may have the potential to protect tobacco seeds and seedlings from P. myriotylum infection. Greenhouse Pythium control trials identified ethaboxam, mefenoxam, and copper ionization as potentially promising alternatives to etridiazole for Pythium disease management in tobacco transplant production. The outcomes of this project provide useful new information to better understand the composition, distribution, and diversity of Pythium communities in tobacco transplant greenhouses and to improve Pythium disease management for tobacco transplant production. / Doctor of Philosophy / Pythium diseases are common in tobacco transplant production and can cause up to 70% seedling losses in hydroponic (float-bed) tobacco transplant greenhouses. However, little is known about the composition and distribution of Pythium communities in tobacco transplant greenhouses. This project began with a tobacco transplant greenhouse survey, in which 12 Pythium species were recovered from center walkways, weeds, greenhouse bay water, and tobacco seedlings. Pythium dissotocum and P. myriotylum were the two types (species) of Pythium most commonly found in the survey. Pythium myriotylum, P. coloratum, and P. dissotocum were aggressive pathogens that suppressed seed germination and caused root rot, stunting, foliar chlorosis, and death of tobacco seedlings. Pythium aristosporum, P. porphyrae, P. torulosum, P. inflatum, P. irregulare, P. catenulatum, and an isolate of P. dissotocum, were weak pathogens causing root symptoms without affecting the upper part of tobacco seedlings. Pythium adhaerens, P. attrantheridium, and P. pectinolyticum did not affect tobacco seeds or seedlings. The symptoms caused by infection by Pythium species differed among host (tobacco) growth stages, except for the most aggressive species, P. myriotylum. High levels of variation were observed among isolates of P. dissotocum, in terms of vegetative growth rate (on V8 agar media) and aggressiveness on tobacco seed and seedlings. Pythium myriotylum was found to co-exist with multiple other Pythium or oomycete species (neighbor isolates) in the same environments within tobacco greenhouses. Significant interactions between P. myriotylum and some neighbor isolates were revealed, and these interactions significantly affect the consequences of P. myriotylum infection of tobacco seeds. Greenhouse Pythium control trials identified two chemical water treatments (ethaboxam and mefenoxam), and a non-chemical water treatment (copper ionization) as potentially promising alternatives to the current standard Pythium control (etridiazole) for Pythium disease management in tobacco transplant production. The outcomes of this project provide useful new information to both better understand the composition, distribution, and diversity of Pythium communities in tobacco transplant greenhouses and to improve Pythium disease management for tobacco transplant production.

The purpose of this study was to find out if ecological races with different growth rates existed in douglas fir. Seeds were collected from six areas by the Forest Service and the author. A study was made of stem and tap root growth utilizing three photoperiods of varying length. Rate of tap root growth was significant for ecotype suggesting selection for tap root length in the plants from utah and Arizona. Each geographical area represents a growth rate ecotype with a wide break in the data on stem growth.

The Mississippi Alluvial Valley (MAV) has undergone losses of bottomland hardwood forests due to agricultural conversion. Hardwood establishment on marginal croplands has been proposed to mitigate effects of deforestation and related loss of carbon-capture potential. However, a possible concern with reforestation is low seedling survival from mammalian herbivory. I surveyed two afforested fields in the MAV of northwest Mississippi to assess damage and mortality from four herbivores on nine species of hardwood seedlings (n = 868). Percentage survival of seedlings was 35%. Mortality of seedlings caused by herbivores was: hispid cotton rat (Sigmodon hispidus; 6.45%), rabbit ((Sylvilagus spp.; 1.95%), pine vole (Microtus pinetorum; 2.99%), and white-tailed deer (Odocoileus virginiana; 0.69%). Of surviving seedlings (n = 316), 10.82% were damaged by cotton rats, pine vole (2.99%), rabbit (8.06%), and deer (7.02%). Green ash (Fraxinus pennsylvanica), water oak (Quercus nigra), and Nuttall oak (Quercus nuttallii) had greatest survival.

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The response of several plant species to heavy metal contaminated soils was evaluated using plant bioassays with a soil substrate. A natural soil was collected from Dinwiddie County, Virginia and soil analysis was performed. The plant species, Lolium multiflorum, setaria italica and Trifolium rep ens latum, Robinia pseudoacacia, Andropogon gerardi, Asclepias syriaca, Echinacea purpurea, Rudbeckia hirta and Festuca rubra were grown in to determine the response to cupric and cadmium chloride in soils (mg Cu/kg soil). A few plant species were grown in small pots in a plant growth chamber for 28 days using control, 10, 30, 100 and 300mg Cu or Cd/kg soil. Germination proved to be less sensitive than root length. S. italica had highest ECSOs. In eu 20.7 and 15.3 in Cd. All plant species were grown for 7 days in 0.3, 1.0, 3.0 10.0, and 30.0mg Cu/kg soil and in control. Germination was not effected by metal concentrations in most species (p=0.07-0.6), except T.repens latum, R. hirta and F. rubra at 30mg/kg (p=0.0007). Root length was significantly effected by Cu concentrations for almost all species (p=0.0001-0.0112). Setaria italica had the highest EC50 at 10.86mg/kg. Robinia pseudoacacia root length was not significantly affected by CU concentrations. The other species had EC50s ranging from 3.74-7.51mg/kg. Both inhibition and stimulation of root growth were observed. Preliminary studies regarding germination rates, fungicides and rangefinding are included. / Master of Science

Root growth potential CRGP) is the measure of seedling ability to grow new roots. Loblolly pine (Pinus taeda L.) seedlings from 5 seedlots were raised at two widely separated nurseries (Summerville, SC and New Kent, VA). In Experiment I, RGP was determined during the fall and winter of 1984-85 and 1985-86, and several morphological traits were measured. In Experiment II seedlings from the same seedlots were lifted during February 1985 and 1986 at both nurseries, and cold stored until subsequent outplanting; subsamples of outplanted seedlings were used for RGP determinations. Root growth potential varied significantly by family, nursery, and lift date, and first order interactions were significant. Generally, the RGP of Summerville raised seedlings was higher than that of New Kent raised seedlings. RGP was not strongly correlated with common measures of shoot morphology, but RGP was consistently well correlated with lateral root dry weight. In Experiment II, RGP varied significantly by family, nursery, and storage duration. The family x nursery effect was the only significant interaction. Summerville raised seedlings had higher RGP on each planting date. During each year RGP declined rapidly in storage. First year survival was significantly correlated with RGP (r = .52, p < .001). Height increment and RGP were also strongly correlated (r = .80, p < .001). These relationships and their implications for nursery management were discussed. / M.S.

Adequate supply and availability of nitrogen (N) is a major factor hindering successful revegetation of reclaimed surface mines. Because topsoils are often unrecoverable, “topsoil substitutes”, made up of overburden materials devoid of N, are often used on reclaimed surfaces. Four replications of seven surface treatments were applied to 12 m² plots of 2:1 sandstone:siltstone material on a reclaimed coal surface mine in Wise Co., VA: 1) fertilizer (168 kg/ha N, 147 kg/ha P, and 139 kg/ha K), 2) limed (7.8 Mg/ha), fertilized topsoil (30 cm deep), 3) 112 Mg/ha sawdust + fertilizer + 336 kg/ha slow-release N, and 4-7) lime-treated municipal sewage sludge at rates of 22, 56, 112, and 224 Mg/ha. Nitrogen availability after 40 mo (as determined by aerobic and anaerobic incubations) in sawdust- and sludge-amended soils was two to 20 times higher than in fertilized and topsoil-amended soils. Moisture holding capacity was nearly twice as high in sawdust-amended soils as in all other treatments. Soil nitrate (NO₃⁻) levels (0-20 cm layer) in excess of 75 μg/g were detected in the 112 and 224 Mg/ha sludge plots 28 mo after application. Stem volumes of three-year-old pitch X loblolly pines (<i>Pinus rigida</i> X <i>taeda</i>) were four times larger in soils amended with sawdust + slow-release N and were twice as large in soils amended with 22 and 56 Mg/ha sludge compared to fertilized-only seedlings. Increased seedling mortality was observed in the 112 and 224 Mg/ha sludge treatments, possibly due to high soluble salt levels and/or manganese deficiencies. The availability of N from sawdust + slow-release N or sludge (≤ 56 Mg/ha) is better than that of inorganic fertilizer for sustained tree growth. These amendments are recommended for reclamation to the extent that they are economically available. / M.S.

Red maple seedlings grown from fruits collected from matched wet and dry sites from three physiographic regions of Virginia were flooded to test whether red maple seedlings derived from wet sites are affected differently by flooding than are seedlings derived from dry sites. Thirteen weeks of soil inundation on seedling growth found no interactions between flooding and maternal hydrologic condition. However, flooding significantly decreased leaf, stem, and root dry matter accumulation as well as height growth, leaf area growth, root to shoot ratio, mean relative growth rate, net assimilation rate, and mean leaf area ratio. Thirteen days of rhizosphere inundation as well as six days of recovery on seedling gas exchange determined that flooding significantly decreased photosynthetic rate and leaf conductance. A larger decrease in photosynthetic rate than in leaf conductance resulted in decreased water use efficiency and leaf limitation. There were no interactions between flooding and maternal hydrologic condition. Fourteen days of flooding decreased root aerobic respiratory capacity and root ethylene evolution, and caused shoot water potential to be less negative. As in the previous studies, no interaction between flooding and maternal hydrologic condition existed. Although rhizosphere inundation negatively affected the growth and physiology of red maple seedlings, there does not appear to exist any genetic differentiation between wet site and dry site populations affording either of the populations enhanced flood tolerance. Rather, red maple appear to have the species wide phenotypic plasticity to survive flooded conditions. / Master of Science