Global ETD Search

1	THE AREA POSTREMA: A POTENTIAL SITE FOR CIRCADIAN REGULATION BY PROKINETICIN 2 INGVES, MATTHEW 20 August 2009 (has links) Little is known regarding the neurophysiological mechanisms by which the neuropeptide prokineticin 2 (PK2) regulates circadian rhythms. Using whole-cell electrophysiology, we have investigated a potential role for regulation of neuronal excitability by PK2 on neurons of the area postrema (AP), a medullary structure known to influence autonomic processes in the central nervous system. In current-clamp recordings, focal application of 1µM PK2 reversibly influenced the excitability of the majority of dissociated AP cells tested, producing both depolarizations (38%) and hyperpolarizations (28%) in a concentration-dependent manner. Slow voltage ramps and ion substitution experiments revealed a PK2-induced Cl- current was responsible for membrane depolarization, while hyperpolarizations were the result of inhibition of an inwardly rectifying non-selective cation current. In contrast to these differential effects on membrane potential, nearly all neurons that displayed spontaneous activity responded to PK2 with a decrease in spike frequency. These observations are in accordance with voltage-clamp experiments showing that PK2 caused a leftward shift in Na+ channel activation and inactivation gating. Lastly, using post hoc single cell RT-PCR technology, we have shown that 7 out of 10 AP neurons depolarized by PK2 were enkephalin-expressing cells. The observed actions on enkephalin neurons indicate PK2 may have specific inhibitory actions on this population of neurons in the AP acting to reduce their sensitivity to incoming signals. These data suggest that PK2 regulates the level of AP neuronal excitability and may impart a circadian influence on AP autonomic control. / Thesis (Master, Physiology) -- Queen's University, 2009-08-18 11:18:05.977 enkephalin circumventricular patch clamp single cell RT-PCR neuropeptide action potential
2	Charakterizace biologických a funkčních vlastností nového typu lidských CD27- paměťových B lymfocytů / Characterization of biological and functional features of a new type of CD27- memory B lymphocytes. Bajzíková, Martina January 2011 (has links) The increased frequencies of two novel B cell populations defined as IgM+ CD19+ CD27- CD21low CD38low CD24+ and IgM+ CD19+ CD27- CD21low CD38low CD24- in peripheral blood of patients with common variable immunodeficiency (CVID) compared to healthy donors were found. The aim was to search for such B cells in patients with rheumatoid arthritis (RA) and their further characterization. The production of immunoglobulin (Ig) mRNA in single B cells was analyzed using flow cytometry, single cell sorting and RT-PCR, IgVH-specific PCR, cycle sequencing and statistical analysis. The study was focused on analysis of variable regions of the heavy chains of Igs and significant differences in the usage of VH, DH and JH gene segments, mutational frequencies, distribution of silent and replacement mutations, length and composition of CDR3 regions, clonal relation and RAG gene expression in above mentioned B cell populations were found. Because of lack of the surface CD27 molecule being regarded as marker of B cells that have undergone antigen-driven germinal reactions, analyzed populations were considered as naive. However, the pattern and type of mutations suggested that these cells could represent a new type of differentiated memory/antigen- experienced B lymphocytes (in CVID less maturated) with the likely role in...
3	The Physiological Role of Serotonergic Transmission in Adult Rat Taste Buds Jaber, Fadi Luc 21 May 2013 (has links) No description available. Neurosciences Serotonin 5-HT 5-HT1A 5-HT3 taste bud gustation chorda tympani immunocytochemistry single cell RT-PCR ATP P2X P2Y purinergic receptors T2R T1R
4	Characterization of olfactory receptor gene expression in the olfactory epithelium of larval Xenopus laevis / Charakterisierung der Expression von olfaktorischen Rezeptoren im olfaktorischen Epithel vom larvalen Xenopus laevis Gliem, Sebastian 25 October 2010 (has links) No description available. 570 Biowissenschaften Biologie Chemorezeptor Geruch Einzelzell-RT-PCR <i>in situ</i> <i>Xenopus laevis</i> Deorphanisierung chemoreceptor olfaction single cell RT-PCR <i>in situ</i> <i>Xenopus laevis</i> deorphanisation 42.15 42.63 42.67 42.82 WA 000: Biologie
5	Function of glial cells in the inhibitory synaptic transmission of the respiratory network / Funktion von Gliazellen für die synaptische Inhibition im respiratorischen Netzwerk Szöke, Katalin 27 October 2005 (has links) No description available. 570 Biowissenschaften Biologie Astrozyten Glia Glyzin Rezeptor Glyzin Transporter respiratorischer Rhythmus Immunhistochemie Oligodendrozyten Patch-Clamp Respiratorisches Netzwerk Atemzentrum Einzelzell RT PCR astrocyte glia glycine receptor glycine transporter respiratory rhythm immunohistochemistry oligodendrocyte patch-clamp respiratory network single-cell RT PCR 44.37 MED 311: Physiologie {Medizin}

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