A utilização de herbicidas pode modificar a dinâmica do nitrogênio nos agroecossistemas e, como consequência, a disponibilidade do nutriente às plantas e microrganismos. Estas mudanças podem ocorrer devido às alterações fisiológicas ocasionadas nas plantas e/ou devido ao efeito dos herbicidas na atividade e composição da comunidade microbiana do solo, a qual tem funções importantes nas transformações do N no sistema. Neste contexto, objetivou-se com este trabalho de tese: 1- avaliar as perdas de nitrogênio do sistema solo-planta, sem distinção às vias de saída, após a aplicação de herbicidas em Pennicetum glaucum, Avena strigosa e Brachiaria ruziziensis; 2- avaliar a concentração de NH4 + na planta, a emissão de NH3 via foliar e a volatilização de NH3 pelo solo após aplicação de glyphosate em B. decumbens; 3 avaliar a exsudação de compostos nitrogenados por B. decumbens após aplicação dos herbicidas glyphosate, glufosinato de amônio e paraquat; e 4 avaliar a mineralização do N da palha de P. glaucum e A. strigosa dessecados com herbicidas e o aproveitamento do N proveniente da palha por plantas de milho. O herbicida glyphosate aumentou a concentração de NH4 + em plantas de B. decumbens e, como consequência, a emissão de NH3 via foliar em duas espécies de plantas do gênero Brachiaria. A volatilização de NH3 pelo solo não foi afetada pela utilização do herbicida glyphosate. As perdas de N do sistema solo-planta foram maiores após aplicação dos herbicidas glyphosate e glufosinato de amônio em P. glaucum, B. decumbens e B. ruziziensis. No entanto, em A. strigosa as perdas não foram afetadas por estas moléculas. Os herbicidas glyphosate e glufosinato de amônio modificaram a partição do N nas espécies estudadas, ocorrendo redução no conteúdo de N nas folhas. As perdas de N do sistema e a partição do N nas plantas não foram afetadas pelo herbicida paraquat, aplicado em B. ruziziensis e A. strigosa. A exsudação de compostos nitrogenados foi maior após aplicação dos herbicidas glyphosate, glufosinato de amônio e paraquat em B. decumbens. A mineralização do N de resíduos culturais de P. glaucum e A. strigosa foi reduzida pela dessecação destas espécies com os herbicidas glyphosate e glufosinato, sendo observado menor conteúdo de N proveniente do fertilizante no milho cultivado em solo com palha proveniente de plantas dessecadas. No entanto, a massa seca de grãos e das plantas de milho não foram afetadas pela utilização de herbicidas na dessecação das espécies utilizadas como cobertura. O carbono da biomassa microbiana e a conversão de amônio em nitrato foram menores no solo com palha de P. glaucum dessecado com herbicidas em relação à testemunha. Com base nos resultados obtidos neste trabalho de tese, conclui-se que os herbicidas glyphosate, glufosinato de amônio e paraquat podem afetar a dinâmica do N nos agroecossistemas, reduzindo a disponibilidade de N às plantas. Estes resultados apontam a necessidade de desenvolvimento de novas pesquisas, visando conhecer a real magnitude dos impactos causados pelo uso de herbicidas nos processos relacionados ao ciclo do nitrogênio. As informações obtidas neste trabalho de tese e pesquisas futuras relacionadas ao tema podem ser úteis para elaboração de estratégias de manejo que aumentem o aproveitamento do N pelas culturas e reduzam o impacto ambiental ocasionado pelo uso de fertilizantes e herbicidas. / Herbicide adoption might modify nitrogen dynamics in agroecossystems and, for consequence, nutrient availability for plants and microorganisms. These modifications may occur because of plant physiologic changes and/or due to herbicide effects on activity and composition of soil microbial community, which has important functions on system N modifications. In this context, thesis was developed with the following objectives: 1- Evaluating nitrogen losses in soilplant system, with no distinction to exit ways, after herbicide application in Pennicetum glaucum, Avena strigosa and Brachiaria ruziziensis; 2 Evaluating NH4 + plant concentration, NH3 emission by leaves and soil NH3 volatilization after herbicide application in B. decumbens; 3- Evaluating nitrogen compounds exudation by B. decumbens after the application of the herbicides glyphosate, ammonium-glufosinate or paraquat; and 4- Evaluating nitrogen mineralization of P. glaucum e B. decumbens straw previously desiccated with herbicides and N-straw usefulness by corn plants. Glyphosate increased NH4 + concentration in plants of B. decumbens and, by consequence, NH3 leaf emission in two plant species of Brachiaria genus. Ammonia volatilization from soil was not affected by application of the herbicide glyphosate. Nitrogen losses of the soil-plant system were larger after glyphosate and ammonium-glufosinate application on P. glaucum, B. decumbens and B. ruziziensis. However, for A. strigosa, the losses were not affected by herbicide application. The herbicides glyphosate and ammonium-glufosinate modified species N partition, reducing N amount in leaves. System nitrogen losses and N plant partition were not affected by the herbicide paraquat, applied on B. ruziziensis and A. strigosa. Nitrogen compounds exudation was higher after application of the herbicides glyphosate, ammonium-glufosinate or paraquat on B. decumbens. Nitrogen mineralization from crop residues of P. glaucum e A. strigosa was reduced by crop desiccation with the herbicides glyphosate and ammonium-glufosinate, being observed smaller N-fertilizer amount in corn grown in soil with straw of these covering crops. Thus, kernel dry mass and whole plant dry mass were not affected by herbicide application for P. glaucum e A. strigosa desiccation. Microbial biomass carbon and ammonium-nitrate conversion were smaller in soil covered with P. glaucum straw previously desiccated with herbicides when compared to the check. Based on the results reached in this thesis, one could conclude that glyphosate, ammonium-glufosinate and paraquat may affect nitrogen dynamics at agroecossystems, reducing N availability for crops. These results indicate the necessity for developing new researches, aiming to know the real magnitude of the impacts caused by herbicides in the processes of nitrogen cycle. The information obtained in the work and in future researches related to the theme might be useful for creating management strategies that increase N crop usefulness and reduce environmental impact caused by herbicide and fertilizer adoption.

O uso de efluente de esgoto tratado (EET) na agricultura apresenta-se como uma alternativa viável por se tratar de uma fonte de umidade e de nutrientes minerais aos sistemas agrícolas, aliado à redução da utilização de água de boa qualidade na irrigação, bem como a diminuição da descarga do EET nos rios, minimizando assim possíveis danos ambientais. Entretanto, deve-se ressaltar a importância do monitoramento contínuo do sistema solo-planta em razão do risco da utilização de águas residuárias, sobretudo aquelas ricas em sódio. Desse modo, esse trabalho teve como objetivo contribuir para o conhecimento dos efeitos da aplicação de EET no sistema solo-cana-de-açúcar, avaliando possíveis alterações químicas do solo e da planta, e a qualidade do caldo. O experimento foi instalado num campo experimental situado em Lins, São Paulo. A adubação mineral de plantio consistiu na aplicação de metade da dose recomendada do adubo nitrogenado, 15 kg ha-1 de N (nitrato de amônio), 120 kg ha-1 de P2O5 (superfosfato simples) e 80 kg ha-1 de K2O (cloreto de potássio). O delineamento experimental foi em blocos completos com parcelas subdivididas no tempo com cinco tratamentos e quatro repetições. Os tratamentos foram: SI, sem irrigação; 100 %, umidade do solo na capacidade de campo; 125, 150 e 200% que corresponderam a 25, 50 e 100% a mais da umidade do solo na capacidade de campo, respectivamente. Foram observados aumentos nas concentrações de sódio (Na) nas folhas, fósforo (P) e cálcio (Ca) no caldo, e potássio (K) no colmo. Para os micronutrientes houve diminuições nas concentrações de manganês (Mn) e zinco (Zn) na folha, no caldo e no colmo, e no caso do ferro (Fe) a diminuição ocorreu no caldo. A irrigação com EET não ocasionou prejuízos a qualidade dos colmos industrializáveis. As maiores produtividades foram observadas nos tratamentos 100 e 150, 247,33 e 232,81 t ha-1, respectivamente. No solo, houve incrementos do pH, das concentrações de P, Na, B e Fe, e um decréscimo nas concentrações de Ca, magnésio (Mg), K e cobre (Cu). Os teores de matéria orgânica (MO) decresceram em todo perfil do solo e, os teores de carbono total (CT) e nitrogênio total (NT) não apresentaram alterações em razão da irrigação com EET. A não utilização da dose completa do adubo nitrogenado não implicou na queda da produtividade da cana-planta. As perspectivas para a irrigação da cana-de-açúcar com EET são promissoras, demandando estudos de longo prazo para confirmação dos resultados até agora obtidos e também para o monitoramento de possíveis prejuízos que possam vir a ocorrer no sistema solo-planta. / The use of treated sewage effluent (TSE) in agriculture is a viable alternative once it is a source of humidity and mineral nutrients to the agricultural systems, allied to the reduction of good quality water consumption for agricultural irrigation, as well as the reduction of the discharge of TSE in the water courses, thus minimizing possible environmental damages. However, it should be standed out the importance of the continuous monitoring of the soil-plant system because of the risks represented by the wastewater use, overall those rich in sodium. In this manner, this work aimed to contribute to the knowledge of TSE application effects in the soil-sugarcane system, evaluating the chemical alterations of the soil and the plant, and also the quality of the soup. The experiment was installed in an experimental area situated at Lins, São Paulo State. The mineral fertilization at planting was half of the recommended nitrogen dose, 15 kg ha-1 of N (ammonium nitrate), and also 120 kg ha-1 of P (single superphosphate) and 80 kg ha-1 of K (potassium chloride). The experimental design was in complete blocks with split plots in time, with five treatments and four replicates. Applied treatments were: SI, without irrigation; 100%, soil humidity at the field capacity; 125, 150 and 200% that corresponded to 25, 50 and 100% more soil humidity than the field capacity, respectively. Increases in the concentrations of sodium (Na) in the leaves, phosphorus (P) and calcium (Ca) in the soup, and potassium in the culms were observed. Considering the micronutrients, reductions on the concentrations of manganese (Mn) and zinc (Zn) in the leaves, culms and soup occurred, while in the case of iron (Fe), observed reductions occurred for the soup. TSE irrigation has not prejudiced the quality of the industrialized culms. The highest yields were observed in the treatments 100 and 150, 247.33 and 232.81 t ha-1, respectively. Increments of the values of pH and concentrations of P, Na, boron (B) and Fe were obtained in the soil, while, in the case of Ca, magnesium (Mg), K and cupper (Cu) concentrations in the soil decreased. The organic matter levels decreased all over the soil profile and, the levels of total carbon (TC) and total nitrogen (TN) were not altered by the irrigation with TSE. The non-use of the mineral N fertilizer complete dose did not imply in the fall of the sugarcane-plant productivity. The perspectives for sugarcane irrigation with wastewater are promising, demanding however long-term studies in order to confirm the results obtained till the present moment, and also to the monitoring of possible damages that may occur in the soil-plant system.

Apesar de o manejo da pastagem ser um componente chave em sistemas pastoris, poucos esforços foram feitos para determinar a intensidade de pastejo adequada para pastagens tropicais, especialmente sob condições irrigadas. O atrativo econômico de sistemas de pastagens irrigadas depende da elevada produtividade da pastagem, o que significa que fertilizantes nitrogenados precisam ser utilizados. Entretanto, para estabelecer medidas efetivas visando o manejo do nitrogênio (N) é necessário entender o balanço entre entradas e saídas de N e a eficiência de ciclagem e transformações do N em sistemas de produção animal em pastejo. Nesse contexto, sete experimentos foram realizados para avaliar o efeito do resíduo pós-pastejo ou de níveis de fertilizante nitrogenado sobre a produção de forragem e a recuperação do N-fertilizante em pastagem irrigada de Panicum maximum cv. Tanzânia. O resíduo pós-pastejo para pastagem de capim Tanzânia irrigada e adubada com N durante o inverno, considerando ciclo de pastejo de 36 dias, deve ser de aproximadamente 1.900 a 2.100 kg/ha de massa seca verde. Para as estações de primavera e verão, a massa de forragem residual deve ser de cerca de 1.700 a 2.700 kg/ha de massa seca verde. Esse manejo assegurou que a produção de folhas e a relação folha/haste fossem próximas do máximo. Parcelas de 1 m 2 , tendo uma touceira do capim em seu centro geométrico, foram adequadas para estudar a recuperação de fertilizante- 15 N, independentemente da intensidade de pastejo e estação do ano. O aumento da intensidade de pastejo resultou em decréscimo na massa da touceira. Quanto menor a massa da touceira, maior a dependência no N-fertilizante. A combinação de elevada umidade do solo, ausência de chuva no dia subseqüente à adubação e alta temperatura determinaram baixa recuperação de 15 N-uréia no sistema solo-planta (< 35% do N aplicado) e elevadas perdas de amônia por volatilização (> 40% do N aplicado) nos níveis de adubação superiores a 80 kg/ha de N durante o verão. A absorção foliar da amônia volatilizada da uréia aplicada ao solo variou de 2,5% (120 kg/ha de N) a 16,4% (40 kg/ha de N) do nitrogênio volatilizado. Com adubações inferiores a 60 kg/ha de N observou-se níveis subótimos de nutrição nitrogenada e tendência de maior discriminação do 13 C durante o verão. / Whilst grazing management is a key component in pastoral systems little effort has been made to determine the adequate grazing intensity for tropical pastures, especially under irrigated conditions. The economical attractiveness of irrigated pasture systems depends on high pasture productivity, meaning that fertilizer nitrogen (N) must be used. However, to make effective changes toward sustainable N management, an understanding of the balance between N inputs and outputs and the efficiency of N cycling and transformation within the grassland system is required. In this context, seven experiments were carried out to evaluate the effect of post-grazing residue or nitrogenous fertilizer levels on herbage production and on fertilizer-N recovery in irrigated Panicum maximum cv. Tanzania pasture. The post-grazing residue for irrigated, N-fertilized Tanzania grass pasture during the winter, considering a 36-day grazing cycle, should be around 1900 to 2100 kg/ha of green dry matter. During the spring/summer seasons the residual stubble mass should be around 1700 to 2700 kg/ha of green dry matter. This management ensured that leaf production and leaf/stem ratio were close to the maximum. A plot-size of 1 m 2 , with a tussock of the grass in its geometric center, was adequate to study 15 N-fertilizer recovery irrespective of grazing intensity and season of the year. Increasing the grazing intensity resulted in decreased tussock mass. The smaller the tussock mass the greater was the reliance on fertilizer-N. The combination of high soil water content, lack of rainfall in the day following fertilization and high temperature determined low urea- 15 N recovery in the soil-plant system (< 35% of applied N) and high ammonia volatilization losses (> 40% of applied N) at fertilizations levels higher than 80 kg N/ha during the summer. The absorption of ammonia volatilized from urea applied to soil varied from 2.5% (120 kg N/ha) to 16.4% (40 kg N/ha) of the volatilized nitrogen. With fertilizations lower than 60 kg N/ha it was observed sub-optimal N nutrition and a trend of higher 13 C discrimination during the summer.

L'Équateur est le 5ème pays producteur de pétrole en Amérique du Sud. Les étapes d'extraction et de raffinage génèrent des déchets contentant des métaux co-émis avec les hydrocarbures aromatiques polycycliques (HAPs), connus pour leurs effets cancérogènes et toxiques chez l'homme. Dans ce contexte et dans le cadre du programme ANR MONOIL, le premier objectif de cette thèse était de déterminer la distribution des métaux dans l'environnement sur les provinces d'Orellana et Sucumbíos (zones d'extraction du pétrole en Amazonie, " NAR ") et dans la ville d'Esmeraldas (raffinerie, sur la côte Pacifique," NPC "). Le 2ème objectif était d'étudier le transfert des métaux depuis le sol ou l'air vers les cacaoyers, largement cultivés en Equateur, et d'en déterminer les impacts sur la santé après ingestion. Enfin, le 3ème objectif était de surveiller la qualité de l'air (PM10) afin d'évaluer l'impact des activités anthropiques et le potentiel oxydatif des particules. Des sols et des cultures ont été collectés dans 31 fermes entre 2014 et 2016. Des échantillons d'air ont été prélevés mensuellement sur 3 sites, à proximité des plateformes pétrolières et de la raffinerie. La composition chimique (teneurs en métaux, carbone organique et élémentaire, ions, sucres, polyols, HAPs) a été déterminée dans les PM10. L'évaluation des risques sanitaires a été réalisée via 3 voies d'exposition. Les effets non cancérogènes et cancérogènes ont été quantifiés à l'aide de l'Indice de Risque (HI) et du Risque Total de Cancer (TCR). La bioaccessibilité du Cd par ingestion a été déterminée avec le test BARGE dans les pâtes et fèves de cacao. La formation d'espèces réactives de l'oxygène (ROS) a été quantifiée par spectrophotométrie en utilisant 3 essais acellulaires: DTT, AA et DCFH. Les résultats montrent que dans 72% des sites, les concentrations en Ba, Co, Cu, Cr, Ni, V et Zn dans les sols dépassent les limites de la législation équatorienne. Pour la plupart des cultures, les concentrations en métaux sont inférieures aux limites de détection, sauf pour le Cd dans le cacao et le Pb dans le manioc, qui dérogent aux normes de qualité alimentaire. Les cacaoyers accumulent le Cd dans les feuilles, les cabosses et les fèves. Comme la teneur en Cd du pétrole est inférieure à la limite de détection, les produits agrochimiques et les intrants organiques peuvent être incriminés. Près de 100% de la teneur totale en Cd dans les pâtes et fèves de cacao est bioaccessible par ingestion. Le risque pour la santé après consommation de chocolat oscille entre faible et élevé, selon la quantité ingérée. Dans les PM10, le Ba et le Mo, traceurs spécifiques des activités pétrolières, ont montré des valeurs beaucoup plus élevées que celles enregistrées dans les sites industriels. Les ratios OC/EC étaient plus élevés en Amazonie, suggérant des émissions biogéniques plus importantes. La teneur en HAPs est plus élevée sur la côte, cependant les niveaux de BaP étaient inférieurs aux limites de l'UE. Les résultats du potentiel oxydatif ont montré que les composés émis par l'industrie pétrolière (HAPs, Ba, Ni, Zn), par la combustion de la biomasse (sucres) dans le NAR ainsi que les traceurs industriels dans le NPC (As, Ba, Ni, NH4+) corrèlent avec la génération de ROS. Les principales voies d'exposition aux métaux sont l'ingestion et l'inhalation et, dans une moindre mesure, le contact dermique avec le sol. Les enfants tout comme les adultes sont vulnérables aux effets toxiques de ces composés. Enfin, d'autres facteurs comme la déforestation, l'agriculture et les émissions naturelles (volcans) dans le NAR mais aussi les industries dans le NPC contribuent également aux dégâts environnementaux constatés et aux effets néfastes sur la santé. / Ecuador is the 5th crude oil producer country in South America. Oil extraction and refining generate toxic waste containing metals co-emitted with polycyclic aromatic hydrocarbons (PAHs), naturally present in crude oil or added during production, and known for their carcinogenic and toxic effects in humans. In this context and as a part of the ANR MONOIL program, the first aim of this PhD research was to determine the distribution of metal(loid)s in the environment in the provinces of Orellana and Sucumbíos (oil extraction, North Amazon " NAR ") and the city of Esmeraldas (oil refining, North Pacific Coast, " NPC "). The second aim was to determine if there was a transfer of toxic metals, such as Cd, from soil or air to cacao crops, widely cultivated in Ecuador, and if the consumption of cacao-based products could imply risks for human health. Finally, the third aim was to monitor air quality over 2 years in both areas to assess the impact of anthropogenic activities and the oxidative burden of particulate matter (PM10). Soils and local crops were collected in 31 small-scale farms, between 2014 and 2016. Monthly PM10 samples were collected in 3 sites, in the vicinity of oil platforms close to the refinery. PM10 chemical composition (contents in metals, organic and elementary carbon (OC, EC), ions, sugars, polyols, PAHs) was determined. Human health risk assessment was performed taking into account ingestion, inhalation and dermal contact. Non carcinogenic and carcinogenic effects were quantified using the Hazard Index (HI) and the Total Cancer Risk (TCR). Bioaccessibility of Cd after ingestion was determined by the BARGE in vitro test in cacao beans and liquors. In aerosols, reactive oxygen species (ROS) formation was quantified using 3 acellular assays: DTT (dithiothreitol), AA (ascorbic acid) and DCFH (2',7'-dichlorodihydrofluorescein). Ba, Co, Cu, Cr, Ni, V and Zn concentrations in 72% of the study soils exceeded the limits of the Ecuadorian legislation. For most of the crops, elements were below the limits of detection but, Cd in cacao and Pb in manioc were above the international standards for food quality. Cacao trees accumulate Cd in leaves, pod husks and beans. Because Cd contents in crude oil were below the detection limits, agrochemical products and natural inputs may also be important sources. Almost 100% of the total Cd content in cacao beans and liquor was bioaccessible by ingestion. The health risk after chocolate consumption was low to high, depending on the ingestion rate and the cacao liquor percentage. In PM10, As, Cd, Ni and Pb were below the EU thresholds. However, Ba and Mo, specific tracers of oil activities, used as weighting agents or catalyzers during drilling and refining, showed values much higher than those recorded in other urban-industrialized sites. PM10 composition depended on oil activity. OC/EC ratios were higher in the NAR (oil extraction) than in the NPC (oil refining), suggesting that biogenic emissions were more important in the Amazon area. PAHs contents were higher in the NPC, but levels of benzo-a-pyrene were below the EU limits. Oxidative potential results showed that compounds emitted by oil industry (PAHs, Ba, Ni, Zn) and by biomass burning (sugars) in the NAR as well as industrial tracers in the NPC (As, Ba, Ni, NH4+) were correlated with ROS generation. Regarding residential exposure, the main routes of exposure to metal(loid)s were first ingestion, inhalation and in a small extent soil dermal contact, being both children and adults vulnerable. Finally, the oil Ecuadorian environment is not only contaminated by oil activities. Other factors like deforestation, agriculture and natural emissions (volcanos) in the NAR and industries in the NPC also contribute to environmental damages and may lead to adverse health effects.

Les espèces exotiques envahissantes végétales sont des plantes introduites et naturalisées hors de leur aire de répartition native et capables de maintenir et d’accroitre leur population. Certaines sont considérées comme transformatrices de par leur effet sur les écosystèmes : leur structure, leur fonctionnement ainsi que leur communauté végétale et animale. Ces transformations peuvent rendre certaines de ces espèces nuisibles de par leurs impacts écologiques et économiques important. Les travaux réalisés dans le cadre de cette thèse et présentés ici ont pour objectif d’approfondir les connaissances sur l’impact des invasions biologiques. La faune du sol, la végétation native et leur substrat ainsi que son fonctionnement ont été étudiés à différentes échelles spatiales. Deux espèces exotiques, envahissantes en Europe, ont été considérées comme modèles pour ces travaux : le robinier faux-acacia (Robinia pseudoacacia) et la renouée du japon (Reynoutria japonica). Premièrement, une méta-analyse globale a permis de démontrer l’effet positif des invasions biologiques végétales sur l’abondance de certains groupes de la faune du sol, notamment les consommateurs primaires, en fonction de la structure de l’habitat (ouvert ou fermé). Ensuite, une étude à large échelle sur le robinier faux-acacia a permis d’illustrer les différences qui peuvent exister dans la réponse des écosystèmes forestiers aux invasions le long d’un gradient latitudinal. Ce gradient, composé de quatre régions distinctes en Europe de l’Ouest présente des différences de climat et de végétation dominante, ces différences modifiant l’impact du robinier faux-acacia. Une étude approfondie sur le robinier faux-acacia en Normandie a permis de mieux comprendre l’effet du robinier faux-acacia sur les communautés animales et végétales ainsi que sur le fonctionnement des écosystèmes par comparaison avec deux essences natives dominantes. Finalement, une manipulation expérimentale en laboratoire a démontré l’impact des composés allélopathiques de la renouée du Japon sur une partie de la faune du sol. Cette étude a montré que certaines espèces exotiques envahissantes sont susceptibles d’influencer la faune, et les réseaux trophiques, du sol par leur métabolisme secondaire. Ces travaux illustrent l’intérêt, dans le contexte des invasions biologiques végétales, de l’étude simultanée des compartiments aériens et souterrains à différentes échelles spatiales. / Invasive alien plants are species introduced and naturalized outside of their native distribution range and which have the capacity to maintain and expand their population. Some of these species are considered to be ecosystem transformers by altering their structure, functioning as well as resident animal and plant communities. These induced alterations make some of these species undesirable through their ecological and economical impacts. The work presented in this thesis aimed at a better understanding of the impact of biological invasions by alien plants. The soil fauna, native vegetation and their substrate, as well as ecosystem functioning, were studied at different spatial scales. Two exotic alien species, invasive in Europe, were considered as biological models for this work: the black locust (Robinia pseudoacacia) and the Japanese knotweed (Reynoutria japonica). Firstly, a global meta-analysis demonstrated the positive impact that plant invasions can exert on the abundance of some groups within the soil fauna, notably primary consumers, within different types of habitats (open or closed). Then, a large-scale study on the black locust revealed the differences that can can occur in the response of forest ecosystems to invasions along a latitudinal gradient. Study sites along this gradient, distributed amog four distinct regions in western Europe, exhibit differences in climate and dominant native vegetation which can alter the impact of the black locust. A detailed study on black locust impact in Normandy demonstrated the impact of R. pseudoacacia on native plant and soil fauna communities, as well as some ecosystem functions, in comparison to two native tree species. Finally, a laboraty experiment demonstrated the impact that allelopathic compounds extracted from Japanese knotweed rhizomes can have on some organisms within the soil fauna. This study showed that some invasive alient plants can influence the soil fauna, and soil food webs, through their secondary metabolism. This thesis illustrates that simultaneous study of both aboveground and belowground ecosystem compartments at different spatial scales is of interest in the context of biological invasions.

In der Arbeit werden nach einer detaillierten Beschreibung der eingesetzten Böden mit erhöhter Konzentration an den Radionukliden 210Pb, 226Ra, 227Ac, 230Th und 238U und der angewandten Analysenverfahren eine umfangreiche Übersicht über die Transferfaktoren Boden - Pflanze für die einzelnen Pflanzenteile der in der menschlichen Ernährung genutzten Gemüse und in Futterpflanzen vorgestellt. Die gammaspektrometrischen Bestimmungen von geringen Radioaktivitäten wurden einem Untertagemesslabor durchgeführt. Die Unterschiede in den Transferfaktoren für die Pflanzen und deren Teile und Böden und die Beziehungen zwischen Transferfaktoren und eluierbaren Anteilen der Böden, Pflanzenteile; sowie zwischen den radioaktiven Elementen Radium und Actinium und den Elementen Barium und Lanthan wurden dargestellt. Es wurde bei den Pflanzen eine Abnahme der Radioaktivität von der Wurzel über Stängel und Blatt zur Frucht festgestellt. Die Transferfaktoren für das bisher kaum bestimmten 227Ac ist relativ hoch und liegt in der Größenordnung der sehr konservativ angesetzten Richtwerte des Strahlenschutzes, während sie für die anderen Radionuklide weit unter den Richtwerten liegen. / The used soils with enhanced activity of the radionuclides 210Pb, 226Ra, 227Ac, 230Th, and 238U and the analytical methods for the determination of soil-plant transfer factors are described and a survey of the obtained values for parts of plants used for human nutrition are presented. Gamma spectrometric determination of low radioactivities has been performed in an underground laboratory. For comparison the factors are determined for the plants of similar elements Ba for Ra and La for Ac. In all cases the values decreases from the roots over the leaves to the fruits. The values for 227Ac are relatively high and are in the magnitude of the „Richtwerte“ for radiation protection, in spite of the other radionuclides, which are lower than the „Richtwerte“.

Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2017-06-09T18:42:59Z No. of bitstreams: 2 mayara_ribeiro_lage.pdf: 7630209 bytes, checksum: fb39ab87647bdca7bc30286669fad378 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2017-06-22T14:54:33Z (GMT) No. of bitstreams: 2 mayara_ribeiro_lage.pdf: 7630209 bytes, checksum: fb39ab87647bdca7bc30286669fad378 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2017-06-22T14:54:33Z (GMT). No. of bitstreams: 2 mayara_ribeiro_lage.pdf: 7630209 bytes, checksum: fb39ab87647bdca7bc30286669fad378 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2016 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Um trecho da BR- 367, que liga a cidade de Diamantina ao distrito de Guinda, em Minas Gerais, perpassa por ?reas de campo rupestre na Serra do Espinha?o Meridional e apresenta diversas ?reas de empr?stimo, constitu?das por jazidas de material granular consumidas em obra rodovi?ria da pr?pria via entre as d?cadas de 1960 e 1980. Atualmente tais ?reas ainda apresentam-se fortemente degradadas, caracterizando-se pela fraca cobertura vegetal, exposi??o do solo e inexist?ncia de um sistema de drenagem superficial eficiente. Neste contexto, surge a necessidade de alternativas que tenham efic?cia na integra??o dos conhecimentos silviculturais e princ?pios ecol?gicos visando o retorno destas ?reas ?s condi??es pr?ximas das originais. Este trabalho teve como objetivo entender a rela??o entre vari?veis ambientais e a estrutura horizontal e flor?stica das esp?cies colonizadoras destes ambientes degradados. Foram selecionadas quatro ?reas degradadas, analisadas por fotografias a?reas referentes aos anos de 1960 e 1979 nas quais foi poss?vel detectar das ?reas degradadas pontuais. Para o estudo do substrato estratificou-se as quatro ?reas selecionadas para a coleta de amostras deformadas e indeformadas de forma preferencial. Obteve-se as propor??es de areia, silte, argila, dados de resist?ncia ? penetra??o, densidade de part?culas e aparente, porosidade total, micro e macro porosidade. Para a an?lises qu?micas foram coletadas entre 5 subamostras de cada estrato com o intuito de se obter uma amostra composta do substrato superficial (0-20 cm), de forma a representar a heterogeneidade ambiental de cada ?rea. Foram analisados: pH em ?gua; teores de P, K+, Mg2+, Ca2+ e Al3+; complexo sortivo (acidez potencial (H+Al), satura??o e soma por bases (V% e SB), CTC (T), CTC efetiva (t) e satura??o por alum?nio (m%) e mat?ria org?nica (MO). Os dados obtidos foram aferidos e submetidos a an?lises estat?sticas. Realizou-se an?lise de componentes principais (PCA) pelo software PCORD vers?o 6, de modo a sintetizar as vari?veis de maior relev?ncia na correla??o das vari?veis analisadas. Para levantamento da comunidade vegetal foi empregado o m?todo da interse??o na linha no qual em cada ?rea foram alocadas doze linhas com 10,0 m de comprimento, distribu?das em zigzag, seguindo as coletas de solo, para correlacionar caracter?sticas do solo e vegeta??o. Cada linha foi subdividida em dez unidades amostrais (UA) cont?nuas, totalizando 470 UAs e anotado a ocorr?ncia de esp?cies de h?bito herb?ceo, arbustivo e arb?reo. Foram calculadoss par?metros fitossociol?gicos: frequ?ncia absoluta (FA), frequ?ncia relativa (FR), cobertura absoluta (CA), cobertura relativa (CR) e o valor de import?ncia (VI). Para comparar os diferentes setores das ?reas estudadas quanto ao perfil de estrat?gias ecol?gicas de esp?cies herb?ceas, estas foram classificadas em sistemas de guildas de acordo com as estrat?gias de regenera??o, estratifica??o e dispers?o. Nas quatro ?reas analisadas foram amostrados 1.517 indiv?duos, pertencentes a 22 fam?lias e 109 esp?cies. A an?lise dos atributos f?sicos e qu?micos do substrato evidenciou alta limita??o ao desenvolvimento de plantas colonizadoras, apresentando como restri??es a alta resist?ncia a penetra??o e substratos de textura arenosa com baixa fertilidade natural. Verificou-se que houve rela??o entre os gradientes ambientais e a abund?ncia e composi??o flor?stica da vegeta??o colonizadora, ficando a maioria das esp?cies mais fortemente correlacionada com as vari?veis MO, H+Al e V al?m de propor??o de Areia, PT, DMG e RP. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2016. / A section of the BR-367, connecting the Diamantina town to the Guinda district, Minas Gerais, runs through areas of rupestrian field of Espinha?o Range and presents several loan areas, consisting of deposits of granular material consumed in road construction between the 1960?s and 1980?s, Currently, these areas are still heavily degraded, characterized by poor vegetation cover, soil exposure and the absence of an efficient surface drainage system. In this context, the need arises for alternatives that have effectiveness in the integration of silvicultural knowledge and ecological principles aiming the return of these areas to the conditions close to the originals. This work aimed to understand the relationship between environmental variables and the horizontal and floristic structure of the colonizing species of these degraded environments. Four degraded areas were selected, analyzed by aerial photographs referring to the years 1960 and 1979 in which it was possible to detect the degraded areas. For the study of the substrate, the four areas selected for the collection of deformed and undisturbed samples were stratified in a preferential way. The proportions of sand, silt, clay, penetration resistance data, particle and apparent density, total porosity, micro and macro porosity were obtained. For the chemical analyzes, 5 sub-samples of each stratum were collected with the aim of obtaining a composite sample of the surface substrate (0-20 cm), in order to represent the environmental heterogeneity of each area. The following parameters were analyzed: pH in water; Contents of P, K +, Mg2 +, Ca2 + and Al3 +; (S), saturation (V%), sum of bases (SB), CTC (T), effective CTC (t) and saturation by aluminum (m%) and organic matter. A PCA was performed by software PCORD version 6, in order to synthesize the variables of greater relevance in the correlation of the analyzed variables. Was used the method of the intersection in the line in which in each area were allocated twelve lines with 10.0 m in length, distributed in zigzag, following the soil collections, to correlate soil and vegetation characteristics. Each row was subdivided into ten continuous sample units (AU), totaling 470 POAs and the occurrence of species of herbaceous, shrub and arboreal habit. Phytosociological parameters were calculated: absolute frequency (AF), relative frequency (FR), absolute coverage (AC), relative coverage (CR), and importance value (VI). In order to compare the different sectors of the studied areas with respect to the profile of ecological strategies of herbaceous species, these were classified in guild systems according to the strategies of regeneration, stratification and dispersion. In the four areas analyzed, 1,517 individuals belonging to 22 families and 109 species were sampled. The analysis of the physical and chemical attributes of the substrate showed a high limitation to the development of colonizing plants, presenting as restrictions the high resistance to penetration and substrates of sandy texture with low natural fertility. It was verified that there was a relation between the environmental gradients and the abundance and floristic composition of the colonizing vegetation, being most of the species more strongly correlated with the variables MO, H+Al and V besides the proportion of Sand, PT, DMG and PR.

O presente trabalho teve como objetivo principal a aplicação de uma metodologia, empregando técnicas de estatística clássica, geoestatística e geoprocessamento, para o mapeamento da variabilidade espacial do potencial produtivo em um talhão com clones de Eucalypt us grandis, aos 6,3 anos de idade, e analisar sua relação com atributos do solo e do relevo, dentro de uma abordagem denominada Silvicultura de Precisão. A investigação foi conduzida numa área de 8,4 ha, com relevo acidentado, pertencente à Suzano Bahia Sul Celulose, em Paraibuna, SP. Foi instalada, na área experimental, uma grade irregular totalizando 41 pontos amostrais, nos quais foram coletados dados referentes às suas coordenadas planas, para georreferenciamento da área de estudo; cotas altimétricas; atributos da planta, mais especificamente DAP e altura total; e amostras de solo na profundidade de 0-20cm, para análises químicas, físicas e de umidade. Inicialmente foram realizados o resumo estatístico e a análise exploratória do conjunto de dados de cada variável, utilizando-se os pacotes estatísticos Excel, SAS e Statistica. Posteriormente foram ajustados, no GS+ e no Geoeas, semivariogramas para as variáveis, definindo-se os parâmetros necessários para a krigagem. Após isso, foi avaliado o número ideal de vizinhos para a krigagem e o erro da estimativa por meio da validação cruzada, utilizando-se as funções disponíveis no Geoeas. Por fim, foram gerados, no Surfer, os mapas de contorno e de superfície contínua (MDT e MDE) das variáveis em estudo. De posse dos modelos digitais das variáveis, foram determinadas estatisticamente, no SIG Idrisi, por meio de análise de correlação e regressão espacial entre mapas (regressão pixel-a-pixel), as correlações e relações entre potencial produtivo (IS) e atributos do solo e do relevo, assim como o ajuste de modelos estatísticos capazes de predizer o valor do índice de sítio por meio dos valores dos atributos do solo e do relevo. Os resultados mostraram que o melhor modelo de regressão linear múltipla espacial ajustado explicou 79% das variações do IS. Todas as variáveis apresentaram dependência espacial, exceto B e MO, sendo que o modelo esférico apresentou o melhor ajuste para os semivariogramas. Também foram determinadas estatisticamente, no Statistica e SAS, por meio de análise de correlação e regressão linear simples, que não leva em consideração a posição espacial dos dados, as correlações e relações entre IS e atributos do solo e do relevo. A partir da análise de regressão linear múltipla (métodos "todas as regressões possíveis" e "passo-a-passo") foram ajustados modelos estatísticos capazes de predizer o valor de IS por meio dos valores dos atributos do solo e relevo. O melhor modelo de regressão linear múltipla ajustado explicou 65% das variações do IS. Os resultados obtidos permitiram identificar os atributos que influenciam no potencial produtivo do talhão, com destaque para: cotas e alumínio, de forma indireta; e umidade, argila total, areia total, areia muito grossa, manganês e soma de bases, de forma direta. Foram identificadas relações entre a variabilidade dos atributos estudados na área experimental, com destaque para a relação entre a distribuição de cotas, areia total, argila total e alumínio com a do Índice de Sítio; e entre a de cotas, areia total, argila total, areia muito grossa e umidade. No geral as áreas de maior potencial produtivo apresentaram menores teores de alumínio, argila total e umidade; maiores teores de manganês, soma de bases, areia total e areia muito grossa; além de ocuparem posição menos elevada no relevo, ou seja, cotas mais baixas. Este cenário determina maior porosidade e permeabilidade ao solo, beneficiando a aeração, penetração e crescimento do sistema radicular, favorecendo a absorção de nutrientes e água pelas raízes, assim como a exploração de reservas hídricas e de nutrientes das camadas subsuperfíciais do solo. / The main purpose of this work was the application of a methodology, using classic statistics, geostatistics and geoprocessing techniques, to the mapping of the spatial variability of productive potential in a stand with clones of 6.3 year-old Eucalyptus grandis, and to analyze its relationship with attributes of soil and relief, by an approach denominated Precision Forestry. The investigation was carried out in an area of 8.4 ha, with hilly topography, belonging to the Suzano Bahia Sul Celulose, in Paraibuna, SP. It was installed, in the experimental area, an irregular grid totaling 41 sampling points, where data were collected related to their plane coordinates, for georreferencing of the study area; as well as altimetric data; attributes of plant, more specifically DBH and total height; and soil samples at the depht of 0-20cm, for chemical, physical and moisture analysis. Initially the statistical summary and the exploratory analysis of the data of each variable were accomplished, being used the statistical packages Excel, SAS and Statistica. Afterwards, the semivariograms for the variables were adjusted, in GS+ and in Geoeas, being defined the parameters needed for the kriging. After that, the ideal number of neighbors was evaluated for the kriging and the error of estimate by cross validation, being used the available functions in Geoeas. Finally, the contour maps and the continuous surfaces (DTM and DEM) of the studied variables were generated, in the software Surfer. With the digital models of the variables, were determined, in the GIS Idrisi, by correlation analysis and spatial regression among maps (regression pixel-by-pixel), the correlations and relationships among productive potential (SI) and attributes of soil and relief, as well as the adjustment of statistical models capable to predict the value of the site index by values of the attributes of soil and relief. The results showed that the best model of adjusted spatial multiple linear regression explained 79% of the variations in the SI. All the variables presented spatial dependence, except B and OM, and the spherical model presented the best adjustment for the semivariograms. Also, the correlations and relationships between SI and attributes of soil and relief were statistically determined, in Statistica and SAS, by correlation analysis and simple linear regression, that do not take into consideration the spatial location of the data. Starting from the analysis of multiple linear regression (methods "all possible regressions" and "stepwise"), statistical models were adjusted, being capable to predict the value of SI by values of the attributes of soil and relief. The best model of adjusted multiple linear regression explained 65% of the variations in the SI. The obtained results allowed identifying the attributes that influence the productive potential of the stand, mainly: altimetric levels and aluminum, in an indirect way; and moisture, total clay, total sand, very coarse sand, manganese and sum of bases, in a direct way. Relationships were identified among the variability of the studied attributes in the experimental area, mainly the relationship among the distribution of altimetric levels, total sand, total clay and aluminum with the Site Index; and among the altimetric levels, total sand, total clay, very coarse sand and moisture. In general, areas with a higher productive potential presented lower contents of aluminum, total clay and moisture; higher contents of manganese, sum of bases, total sand and very coarse sand. They are at the lowest locations in the relief, in other words, lower altimetric levels. This scenery determines higher porosity and permeability to the soil, allowing better aeration, penetration and growth of the root system, favoring the absorption of nutrients and water by the roots, as well as the exploration of water and nutrients reserves in the subsuperficial soil layer.

Este trabalho teve como objetivo estudar a influência de diferentes doses e períodos de aplicação de CO2, via água de irrigação por gotejamento, no desenvolvimento e no estado nutricional da alface, na produtividade, avaliando também os efeitos na solução e no fluxo de CO2 do solo, no pH da água de irrigação e na concentração na atmosfera. Os experimentos foram realizados em diferentes períodos durante dois anos (1999 e 2000), no Horto do Departamento de Ciências Biológicas da ESALQ/USP, localizado em Piracicaba-SP à latitude de 22º 42' 30' S e longitude de 47º 30' 00'. A primeira etapa constituiu de testes preliminares para determinação da concentração de CO2 que refleti-se em resposta à fotossíntese da cultura da alface. A segunda e a terceira etapa foram representadas por quatro experimentos com a finalidade de testar as doses de CO2, determinadas na primeira etapa, e observar os efeitos provocados pela sua aplicação via água de irrigação na alface, na atmosfera, no solo e na água de irrigação. Nestas etapas os experimentos foram conduzidos sob túneis plásticos, e em delineamento experimental inteiramente casualisado. Os tratamentos foram compostos por diferentes doses de CO2 - 0, 52, 155 e 310 kg ha-1 e distintos períodos de aplicação - TT, T3/4, T1/2, T1/4 referentes, respectivamente, ao total e aos ¾, ½ e ¼ finais do ciclo da cultura. Em um dos experimentos foi aplicado CO2 no ambiente, na dose de 155 kg ha-1. As irrigações foram realizadas diariamente com base na evaporação do tanque classe A, corrigidas pelo coeficiente da cultura e do tanque. O monitoramento do CO2 atmosférico foi realizado nos tratamentos que receberam CO2 via água e via ar. A concentração de 600 micromol de CO2 mol-1 aumentou a fotossíntese da cultura da alface. A aplicação de CO2 via água de irrigação modificou o desenvolvimento das plantas de alface. As doses de 52 e 155 kg ha-1 de CO2 aplicadas via água promoveram aumentos na produtividade para as duas cultivares (Elisa e Verônica), atingindo valores de até 26%, quando comparado à testemunha, de forma diferente à aplicação da dose de 310 kg ha-1 que não proporcionou resposta positiva no desenvolvimento das plantas. As análises do solo mostraram alterações pela aplicação do CO2 através da água, sendo que o íon potássio foi modificado na solução do solo e na parte área da planta, o fluxo de CO2 no solo cresceu com o aumento da dose de CO2 aplicada e o pH do solo mostrou uma tendência ao abaixamento, juntamente com o pH da água da irrigação. A aplicação de CO2 via água não alterou a concentração do CO2 ambiente. Com a aplicação via ar, o aumento do CO2 foi simultâneo à aplicação, retornando ao normal, após finalizada à operação. O período mais adequado para aplicação do CO2, durante o ciclo da cultura, foi verificado no ¼ final do ciclo de cultivo. A análise econômica mostrou a viabilidade da aplicação de CO2 via água de irrigação para a cultura da alface, nas condições em que os experimentos foram conduzidos. / The aim of this work was to evaluate the influence of different CO2 application rates and periods via drip irrigation water on the development and nutritional state of lettuce plants, its productivity, further to the effects on the solution and CO2 flow in the soil, irrigation water pH and on the concentration in the atmosphere. The experiments were carried out in different periods for two years (1999 and 2000), at the Garden of the Biological Sciences Department of ESALQ/USP, in Piracicaba-SP, at 22º 42' 30' S latitude and 47º 30' 00' longitude. The first stage included preliminary tests to determine the CO2 concentration whose response would reflect the photosynthesis of the lettuce crop. The second and third stages were represented by four experiments to test the CO2 rates, determined at the first stage, and to observe the effects provoked by its application via irrigation water on the lettuce crop, on the atmosphere, on the soil and on the irrigation water. At these stages the experiments were conducted under plastic tunnels in a completely randomized block design. The treatments included CO2 rates - 0, 52, 155 and 310 kg ha-1 and distinct application periods - TT, T3/4, T1/2, T1/4 referring to the total and final ¾, ½ and ¼ of the crop cycle, respectively. CO2 was also applied to the environment in one of the experiments at a 155 kg ha-1 rate for comparative analysis with the CO2 applied via irrigation water. The irrigation was daily performed based on a class A evaporation tank, amended by the crop and tank coefficient (Kc and Kp, respectively). The CO2 monitoring was performed in the treatments receiving CO2 via irrigation water and via air. The 600 micromol CO2 mol-1 provided increased photosynthesis in lettuce. The CO2 application via irrigation water modified the development of the lettuce plants. The 52 and 155 kg ha-1 CO2 rates applied via water promoted an increased productivity for both cultivars studied, reaching values of up to 26% in comparison with the control plant, differently from the 310 kg ha-1 application rate, which failed to provide a positive response in the development of the plants. The soil-related analyses showed changes after the CO2 was applied through irrigation water, and the potassium ion was modified in the soil solution and in the aerial part of the plant, the CO2 flow in the soil increased with the higher applied CO2 rate, and the soil pH tended to decrease, together with the irrigation water pH. The application of CO2 via water did not change the concentration of the environmental CO2. With the CO2 application via air the atmospheric CO2 increased simultaneously with the application, returning to the normal concentration when the operation was completed. The most adequate period for CO2 application during the development cycle of the crop was verified at the last quarter of the cultivation cycle. The economical analysis indicated the viability of CO2 application via irrigation water for the lettuce crop under the conditions in which these experiments were conducted.

Les bassins de gestion des eaux pluviales, dont l’horizon de surface est parfois fortement contaminé, peuvent être naturellement ou volontairement colonisés par la végétation. L’objectif de ce travail est de mieux comprendre le rôle de la végétation dans le contrôle de la mobilité des métaux lourds comme le Zinc, le Cuivre et le Cadmium. Le premier volet de ces travaux, consacré à la caractérisation de la végétation se développant dans ces bassins, a montré que ces ouvrages abritaient une flore diversifiée, allant d’une végétation rudérale éparse à une végétation dense caractéristique de zones humides. Ensuite, une analyse physico-chimique multiparamétrique d’échantillons prélévés à la surface de bassins colonisés par des espèces végétales dominantes a été réalisée. Nous avons montré que les carbonates et la matière organique contrôlaient particulièrement la mobilité du Cu, Cd et Zn dans ces milieux. Les paramètres les plus variables temporellement et spatialement, sont essentiellement liés au cycle de la matière organique (nitrates, fraction métallique lié à la matière organique) mais également à l’apport de particules anthropiques (texture, métaux). Lorsque la plante est à son maximum de croissance, la mobilité potentielle des métaux est la plus élevée alors que la phase de sénescence de la plante semble conduire à un meilleur piégeage de ces contaminants. Cette thèse invite à considérer la végétation comme un agent de transformation de l’horizon de surface et à intégrer cette composante dans les futurs travaux sur la contamination des ouvrages d’assainissement urbain. / Urban stormwater basins surfaces can be highly contaminated and can be spontaneously or intentionaly vegetated. The aim of this work is to better characterize the role of the vegetation on heavy metal (Zn, Cd, Cu) mobility. Firstly, vegetation inventory has pointed out the high species diversity in these devices (from ruderal vegetation to wetland plants). Then, a physico-chemical characterization of surface samples vegetated by dominant species has been carried out. We have shown that carbonates and organic matter play a major role in the control of heavy metal mobility. The parameters which are the most spatially and temporally variable are essentially related to organic matter cycle (nitrate, heavy metal fraction bound to organic matter), and to the introduction of stormwater suspended matter (heavy metals, texture). At the maximum of plant growth phase, heavy metal mobility is enhanced and the period of plant death leads to a better heavy metal retention in the basin surface. This thesis invites us to consider plants as agents of surface stormwater basin transformation and to include plants in future research work.