Nectarivorous hummingbirds subsist almost exclusively on a mixture of sucrose, glucose and fructose found in floral nectar. Previous studies have shown that hummingbirds can fuel hovering flight almost exclusively using recently ingested sucrose. However, the relative capacities for the direct utilization of glucose and fructose by hovering hummingbirds remain unknown. 13C-enriched solutions of glucose and fructose were administered separately. Exhaled breath samples were collected using feeder-mask respirometry and sent for subsequent mass spectrometric analysis. I found hovering hummingbirds transition from exclusively oxidizing endogenous fatty acids when fasted, to oxidizing newly ingested carbohydrates when given access to either glucose or fructose solutions. Interestingly, the amount ingested, fractional turnover of stable carbon isotope signatures, amount oxidized, energy expended and proportion of hovering metabolism supported by each hexose, were each similar between glucose and fructose. These results demonstrate hovering hummingbirds’ ability to utilize fructose and glucose equally.

No description available.

The Petrockstowe and Bovey basins are two similar pull apart (strike slip) basins located on the Sticklepath – Lustleigh Fault Zone (SLFZ) in Devon, SW England. The SLFZ is one of the several faults on the Cornubian Peninsula and may be linked to Variscan structures rejuvenated in Palaeogene times. The bulk of the basins’ fill consists of clays, silts, lignites and sands of Palaeogene age, comparable to the Lough Neagh Basin (Northern Ireland), which is also thought to be part of the SLFZ. In this study a multiproxy approach involving sedimentary facies analysis, palynological analysis, stable carbon isotope (δ13C) analysis and organic carbon palaeothermometer analyses were applied in an attempt to understand the depositional environment in both basins. A negative carbon isotope excursion (CIE) with a magnitude of 2‰ was recorded at ~ 580 m in the siltstone, silty clay to clay lithofacies in the lower part of Petrockstowe Basin, with minimum δ13CTOC values of -28.6‰. The CIE spans a depth of 7 m. Palynological characteristics of this excursion are correlated with the Cogham Lignite in the southern UK, which is the only established PETM section in the UK, and other continental sections to test whether the palynology associated with this CIE can be used to date it. The age model proposed herein correlates the CIE to the Eocene Thermal Maximum -2 (ETM2; ~ 52.5Ma) event. Key pollen and spore assemblages found in the lower Petrockstowe Basin are Monocolpopollenites, Inaperturopollenites, Laevigatisporites, Bisaccate conifer pollen and Tricolporopollenites, which suggest an Eocene age, while those occurring in the upper part of the Petrockstowe and Bovey basins are Arecipites, Inaperturopollenites, Monocolpopollenites, Tricolporopollenites, Sequoiapollenites, and Pompeckjodaepollenites, which have suggested botanical affinities to modern tropical to sub-tropical genera signifying a climate that was frost-free at the time of sediment deposition. This assemblage further suggests that these sediments are Oligocene to middle Oligocene in age. In the upper part of the Petrockstowe Basin, reconstructed mean annual air temperatures (MAT) demonstrate a clear departure from the mean temperature of 24.5oC at 10 m to 19.5oC towards the top of the core, indicating a steady continuous decline similar to the temperature departures seen in the Solent Group in the Hampshire Basin, Isle of Wight, UK which has an established Eocene – Oligocene succession.

Changes in marine production play a key role in determining the trophic structure of the northeastern Pacific Ocean. This is a region of great environmental fluctuations due to modern, historical, and paleo-environmental variability recorded throughout the Holocene. These fluctuations are recorded in the bone collagen of the marine mammals that reside in these waters. Marine mammal remains from four previously excavated archaeological deposits on Unalaska Island, Alaska are used as a proxy for marine production changes throughout the Holocene (4,500 BP to 350 BP). Historic and modern samples from museum collections, subsistence harvests, and previously published data provide a distinct contrast to prehistoric marine mammals. Stable carbon and nitrogen isotope ratios (δ13C and δ15N) derived from marine mammal bone collagen correlate to changes in marine production and food web length. The 13C and 15N of prehistoric marine mammal taxa covary through the Holocene, indicating no trophic level change with fluctuations in 13C. Changes in δ13C and δ15N of marine mammals are correlated to periods of environmental fluctuations within the Holocene. Cooler climatic periods (transitional interval, beginning of the Neoglacial Interval, and Little Ice Age) show enrichedδ13C, reflecting primary production increase, compared to warmer climate periods (end of the Neoglacial Interval into the Medieval Climatic Anomaly). Unidentified cetacean bones are isotopically distinguishable into orders Mysticeti (baleen) and Odontoceti (toothed) due to different feeding ecologies. The δ13C depletion in modern pinnipeds compared to prehistoric is likely caused by the effect of increased anthropogenic atmospheric CO2 and resulting decrease in primary production.

Establishing a chronology of variation in isotopic values can reveal frailty associated with biological and social age, as well as highlight individuals who vary from typical patterns. Although general dietary characteristics and infant feeding practices were previously unknown for subadults excavated from the cemetery at Alytus, Lithuania (14th-18th centuries), previous research concludes that Alytus' subadults experienced high rates of physiological, metabolic, non-specific stress, in addition to specific diseases like tuberculosis. To investigate nuanced relationships between diet and mortality, nitrogen and carbon stable isotopes from the femoral and humeral midshaft diaphyses of 70 subadults (32 weeks gestation to16 years) were analyzed. Dietary reconstruction reveals that on average, exclusive breastfeeding continued until around 2 years of age when enriched ?13C (-19.6‰) and ?15N values (12.7‰) begin to deplete suggesting introduction of C3 grain gruels and potential weaning-associated infirmity. Nitrogen values remained slightly elevated in children (3-5 years, 11.2‰) until the beginning of juvenility (5-8 years, 10.3‰) when ?15N more closely mirrored adult values (16 years, 10.2‰), consistent with predominant consumption of terrestrial animal protein, possibly with riverine influence. The difference between femoral to humeral ? F-H 13C (-0.05 ±0.25‰, 1?) and ? F-H 15N (- 0.01±0.45‰, 1?) was not significant, though humeral values were on average more enriched. Enrichments in humeral nitrogen and carbon coincided with estimated weaning age. Cohorts experiencing childhood and adolescent growth spurts experienced higher femoral ?13C and ?15N values. Examining dietary experience and physiological changes contributes a holistic understanding of subadult morbidity and mortality experiences in Medieval Lithuania.

No description available.

No / Gas chromatography (GC), GC-mass spectrometry (GC-MS) and GC-combustion-isotope ratio MS (GC-C-IRMS) analyses of absorbed and surface lipid residues preserved in potsherds were used to explore the extent of pig product processing exploitation in the later British Neolithic Grooved Ware tradition. Assessments were made regarding whether porcine lipids were associated with specific Grooved Ware traits, i.e. decoration, substyle, geographical area and type of site. Two hundred and twenty-two Grooved Ware potsherds were analysed, 70% of which contained lipid concentrations considered significant (>5 μg g−1). All the lipid residues were dominated by animal fats, although plant and beeswax were also detected in a small number of extracts. δ13C values of the major fatty acid components of degraded animal fats (C16:0 and C18:0) were determined for 126 extracts and used to assign ruminant or porcine origins to the residues; 16% of these were found to have a predominantly porcine isotope signature. Statistical associations with pig exploitation were shown to exist with substyle, geographical area and site type, whereas, no relationship was seen between decoration and the type of commodity processed. Intact triacylglycerols were preserved in 19% of the sherds; half of these had distributions consistent with the identifications based on δ13C values, the remainder differed either due to the presence of mixed commodities or because lower molecular weight homologues had been lost due to degradation. In addition to the detection of pig exploitation, results from lipid residue analysis showed a good correlation with faunal assemblages, suggesting that stable isotope analysis may be used as a proxy for animal exploitation at sites where bones have not survived.

The work presented in this dissertation explores the nature of agro-pastoral strategies developed by Neolithic farmers as a way to understand how early food production was inter-twined with environmental and socio-economic opportunities and constraints. Towards this end, a multi-isotope approach is used to address questions of scale and intensity of crop cultivation and animal management at the archaeological sites of Kouphovouno, southern Greece, Makriyalos, northern Greece, and Çatalhöyük, south-central Turkey. Measurements of stable carbon, nitrogen, oxygen and strontium isotope values of carbonized plant remains, human and animal bone collagen and animal tooth enamel are used to examine the similarities and differences in the types of treatments that individual species of plants and animals received during the agricultural cycle at the distinct locations. The results show that farmers at the three sites developed variable methods for exploiting the arable and pastoral landscape and catering to their economic and culinary needs. The discussion considers the implications of these findings to our understanding of the complexity and adaptability of early farming systems.

This thesis investigates the relationship between climate, agriculture and social change in South Asia’s Bronze Age urban Indus Civilisation. Specifically, my research tests the hypothesis that an abrupt weakening of the Indian Summer Monsoon ca 2100 cal BC led to increasing crop water stress, and hence potentially contributed to the Civilisation’s decline by reducing food supply. This hypothesis is frequently invoked in discussions of the Civilisation’s end, yet until now, has not been empirically tested. Using material excavated from several Indus settlements, this study uses a novel combination of isotopic techniques to directly test the connection between climate change and agricultural stress. These techniques are first, oxygen isotope analysis of faunal bones and teeth; and second, stable carbon isotope analysis of crop remains. The oxygen analyses provide detailed records of monsoon intensity at a local, human scale, while the carbon analyses provide an empirical test of whether crop water stress increased. Applied in parallel across a diverse suite of Indus sites, these techniques together provide an archaeologically and ecologically-nuanced analysis of climatic impacts. The archaeological analyses are supported by a methodological study, which investigates how water status relates to the stable carbon isotope signature in barley (Hordeum vulgare) and the Indian jujube (Ziziphus mauritiana) along a climatic transect in north-western India today. Overall, the isotopic results suggest that at the sites sampled here, climate change probably had minimal impacts on crop water availability. This does not necessarily mean that climate change had no impacts on agriculture across the greater Indus sphere, and indeed there are hints that there may have been climatic stress in more vulnerable settings. However, at the sites studied here, any hydrological consequences of climate change—including the 4.2 ka event—appear to have had neither a lasting nor a pervasive impact on the adequacy of crop water supply. This is an important finding, and necessitates a clear refinement of how we think about climatic sensitivity, climatic vulnerability, and climatic impacts across—and indeed beyond—the greater Indus.

Tropical forests provide important habitat for a tremendous diversity of plant and animal species. However, limitations in measuring and monitoring the structure and function of tropical forests has caused these systems to remain poorly understood. Remote-sensing technology has provided a powerful tool for quantification of structural patterns and associating these with resource use. Satellite and aerial platforms can be used to collect remotely sensed images of tropical forests that can be applied to ecological research and management. Chapter 1 of this article highlights the resources available for tropical forest remote sensing and presents a case-study that demonstrates its application to a neotropical forest located in the Petén region of northern Guatemala. The ancient polity of Tikal has been extensively studied by archaeologists and soil scientists, but little is known about the subsistence and ancient farming techniques that sustained its inhabitants. The objective of chapter 2 was to create predictive models for ancient maize (Zea mays L.) agriculture in the Tikal National Park, Petén, Guatemala, improving our understanding of settlement patterns and the ecological potentials surrounding the site in a cost effective manner. Ancient maize agriculture was described in this study as carbon (C) isotopic signatures left in the soil humin fraction. Probability models predicting C isotopic enrichment and carbonate C were used to outline areas of potential long term maize agriculture. It was found that the Tikal area not only supports a great variety of potential food production systems but the models suggest multiple maize agricultural practices were used.