Thesis (MScEng)--Stellenbosch University, 2013. / ENGLISH ABSTRACT: The use of electrodynamic tethers on-board satellites is an exciting scientific prospect. These conductive tethers provide the means for satellites to generate power and to do propulsion by electrodynamic interaction with the geomagnetic field. Although well researched in theory, the concept has not enjoyed much success in practice. This study aims to utilise low-cost CubeSats as experimental tool to verify many of the theoretical principles that govern the behaviour of conductive tethers in orbit. The study provides a theoretical background of the concept by evaluating past tether missions and analysing existing theory. A feasible application of an electrodynamic tether within the size and weight limitations of a Nano-satellite is formulated. Existing theoretical work is adapted to model the dynamics and electrodynamics of specifically Nano-satellites. Using these mathematical models, control and estimation algorithms are designed which would provide stable deployment of a tethered CubeSat pair and stable control of the orientation of the tethered system. To be able to implement these algorithms on a satellite mission, a prototype of a sensor capable of measuring the angle of the tether using a CMOS camera is designed and built. A hardware platform is built to test the deployment of the tether using an electric motor. Electronics are designed to control the operation of the camera, to do motor control, and to run control and estimation algorithms. Using the results obtained from the practical tests done on the hardware, and using the theoretical models and control algorithms designed, a full orbital simulation of the deployment was done. This simulation includes the performance of the deployment system, the electrodynamic performance of the tether in earth‟s plasmasphere, and the estimation and control algorithms to control the system. Different deployment strategies are analysed and their performance are compared. / AFRIKAANSE OPSOMMING: Die gebruik van elektrodinamiese toue aanboord satelliete is 'n opwindende wetenskaplike vooruitsig. Hierdie geleidende toue verleen aan die satelliete die vermoë om krag op te kan wek en propulsie deur elektriese interaksie met die geomagnetiese veld te kan doen. Alhoewel dit goed nagevors is in teorie, het die konsep nog nie veel sukses in die praktyk geniet nie. Hierdie studie het dit ten doel om lae-koste CubeSats aan te wend as 'n eksperimentele instrument om baie van die teoretiese beginsels wat geld vir die gedrag van geleidende toue in wentelbane te verifieer. Die studie bied 'n teoretiese agtergrond van die konsep deur die evaluering van vorige tou-missies sowel as die analise van bestaande teorie. 'n Uitvoerbare toepassing van 'n elektrodinamiese tou binne die grootte- en gewigsbeperkinge van 'n Nano-satelliet is geformuleer. Bestaande teoretiese werk is aangepas om die dinamika en elektrodinamika spesifiek van toepassing op Nano-satelliete, te modelleer. Deur hierdie wiskundige modelle te gebruik, is beheer- en afskattingsalgoritmes ontwerp wat stabiele ontplooiing van 'n verbinde CubeSat-paar en stabiele beheer van die oriëntasie van die verbinde stelsel sal verseker. Om hierdie algoritmes te implementeer op 'n satelliet-sending, is 'n prototipe van 'n sensor wat in staat is om die hoek van die tou met behulp van 'n CMOS kamera te meet, ontwerp en gebou. 'n Hardeware platform is gebou om die ontplooiing van die tou met behulp van 'n elektriese motor te toets. Elektronika is ontwerp om die kamera te beheer, motor beheer te doen asook om beheer- en afskattingsalgoritmes uit te voer. Deur gebruik te maak van die resultate wat verkry is tydens die praktiese toetse wat gedoen is op die hardeware, en deur gebruik te maak van die teoretiese modelle en beheeralgoritmes wat ontwerp is, is 'n volle wentelbaan-simulasie van die ontplooiing gedoen. Hierdie simulasie sluit die gedrag van die ontplooiingstelsel, die elektriese gedrag van die geleidende tou in die aarde se plasmasfeer, en die afskatting- en beheeralgoritmes om die stelsel te beheer in. Verskillende ontplooiingstrategieë word ontleed en hul gedrag word vergelyk.

The mooring of vessels and other floating bodies at sea, such as offshore platforms has necessitated the development of specialised moorings technology. The marine renewable energy (MRE) sector is now at a stage in its development whereby floating devices are adding new challenges to the moorings industries. Floating MRE devices are smaller than, for instance offshore platforms, and are usually targeted for deployment in highly energetic environments. The extreme conditions and the highly dynamic response of an MRE device present challenges in terms of peak loading within the mooring system itself and load transfer to the floating body. Compliant mooring systems provide advantages by reducing the peak loads and fibre ropes are an important asset in achieving such compliance. However, the extent to which existing fibre ropes can safely extend axially to provide compliance is insufficient and is strongly associated to the minimum breaking load (MBL) of the rope. A novel fibre rope mooring tether is presented here that provides advantages over existing ropes. The tether employs a hollow fibre rope containing an elastomeric core, this mechanism de-coupling the extension properties from the strength of the line. The load path is carried through the polyester rope which is terminated conventionally by eye splices, thus minimising any new risks to reliability. Very low axial stiffness is achieved and is shown to be selectable within limits. For comparison, the prototype tether’s MBL of 222 kN is assigned to polyester and Nylon reference ropes. The axial stiifness of these ropes are 590 kN and 463 kN respectively when measured by a secant between the origin and 30% MBL; the novel tether displays an axial stiffness of 72 kN by the same method. This enables the novel tether to achieve more than two and a half times the extension of a comparable Nylon rope in its working range. Numerical modelling of a moored installation demonstrates a threefold reduction in peak load magnitude compared to the existing Nylon rope solution. The tether exhibits two distinct stages of extension, the first having very low axial stiffness. It is demonstrated that the extent of this soft phase can be selected by design and that this might add another useful element of control to moorings design work.

Orbital debris poses a serious threat to ongoing operations in space.  Recognising this threat, the European Commission has funded the three-year Technology for Self Removal (TeSeR) project with the goal of developing a standard scalable Post Mission Disposal (PMD) module to remove satellites from orbit following the completion of their mission.  As the project coordinator and key member of the TeSeR Project, Airbus Defence and Space Germany will invest significant resources in achieving this goal over the course of the project. This thesis details the initial analysis of potential PMD module designs conducted by the author during an internship within the AOCS/GNC department of Airbus Defence and Space Friedrichshafen between 1 April 2016 and 31 August 2016.  Three main concepts, drag sails, drag balloons and Electrodynamic Tethers (EDTs), were evaluated during this time with an emphasis on determining the ability of each design to permit passive attitude stabilisation of the satellite during PMD.  Following the required modification of a pre-existing MATLAB/Simulink model, several key findings were made for each device concept.  It was found that no drag sail designs investigated permitted passive aerodynamic attitude stabilisation at orbit heights above 550 km.  When deorbiting from 800 km, however, the lack of the desired and stable attitude was not found to have a significant increase on the deorbit time or the area‑time product. Drag balloon designs were predicted to be comparatively unstable and less mass efficient for deorbiting purposes, with area‑time products up to approximately 50 per cent higher than the equivalent mass drag sail designs.  In spite of this, unstable drag balloons were found to provide shorter deorbit times than stable balloons due to the contribution of the satellite body and solar array to the total frontal area of the satellite.  This indicated that attitude stabilisation is not required for satellites equipped with drag balloon devices. Modelling of bare EDTs suggested that tethers with lengths of 1000 metres or more would not permit passive attitude stabilisation at an orbit height of 800 km.  Simulation of a 500 metre EDT, however, indicated that passive attitude stabilisation can be achieved with EDT devices and proved that EDTs can generate significantly higher drag forces than aerodynamic devices while possessing a significantly lower device mass.  Following the analysis of these results, a recommendation was made for future work to be aimed at improving the EDT model used in this investigation.

As society draws closer to the post-antibiotic era and the pipeline for alternatives dries, there is an urgent need for the development of novel antimicrobial therapies that do not promote bacterial resistance, particularly for immunocompromised chronic wound patients. Antimicrobial peptides (AMPs), including human-derived LL37, show considerable promise as broad spectrum alternatives that also have wound healing properties; however, few have been clinically implemented as novel antimicrobials due to their cytotoxicity stemming from a poor understanding of their mechanisms and low stability in vivo. It has been suggested that tethering, or attaching AMPs onto surfaces, is a viable strategy of delivering bioactive AMPs to surfaces while reducing cytotoxicity and improving stability. Thus, we designed new chimeric versions of LL37 with collagen-binding domains (CBD), derived from collagenase (cCBD-LL37) and fibronectin (fCBD-LL37) for non-covalent tethering onto collagen, a prevalent biopolymer in commercially available wound dressings and scaffolds. Our overall hypothesis was that CBDs would mediate stable tethering of broadly active, non-cytotoxic CBD-LL37 onto collagen-based scaffolds. We first studied the loading, release and bioactivities (e.g. antimicrobial activity and cytotoxicity) of each CBD-LL37 on commercially available 100% collagen type I PURACOL® wound scaffolds. We found that both cCBD-LL37 and fCBD-LL37 bound highly to collagen, were active against relevant wound pathogens, demonstrated stable activity after 14 days of release, and were not cytotoxic to human fibroblasts. The addition of different CBDs onto LL37 also markedly altered their soluble bioactivities. Using similar methods, we then studied the loading, release and bioactivity of each CBD-LL37 on a commercially available FIBRACOL® wound scaffolds, comprised of 90% collagen type I and 10% calcium alginate biopolymers. We found that both cCBD-LL37 and fCBD-LL37 also bound highly to and retained on collagen for 14 days, but were only active against Gram-negative P. aeruginosa. This suggested that the presence other biopolymers in addition to collagen, which is common among commercial wound dressings, could cause significant differences in binding, retention and bioactivities of CBD-LL37. To better understand how CBD modification affected CBD-LL37 structure leading to different bioactivities, we studied the CBD sequence-, peptide structure-, concentration-, time-, and bilayer composition-dependent interactions of soluble CBD-LL37 and compared these findings with the properties of unmodified LL37. Using Molecular Dynamics (MD) simulations, circular dichroism (CD) spectroscopy, quartz crystal microbalance with dissipation (QCM-D), and fluorescent bilayer imaging we determined the structural basis behind CBD alterations in bioactivities. MD and CD, in addition to other intrinsic CBD properties (helicity, amphiphilicity, charge) we hypothesized that cCBD-LL37 utilized similar mechanisms as unmodified LL37 while fCBD-LL37 demonstrated based primarily on surface adsorption. We used QCM-D and Voigt-Kelvin viscoelastic modeling to determine the time- and concentration-dependent interactions of unmodified LL37 with model mammalian lipid bilayers, the mechanisms of which are still controversial in literature despite being widely studied. These results were used to propose a model for the interaction mechanism of LL37 with zwitterionic bilayers that aligned with its bioactive concentrations. LL37 adsorbed at concentrations where it is immunomodulatory until reaching a threshold which corresponded with its antimicrobial concentrations. The threshold was correlated to lipid bilayer saturation, after which LL37 formed transmembrane pores. We observed collapse of the bilayer into a rigid proteolipid film at concentrations higher than the reported cytotoxic threshold of LL37. The mechanistic and structural information for each CBD-LL37 and unmodified LL37 provided a baseline for QCM-D and Voigt-Kelvin viscoelastic modeling to further elucidate the time-, concentration-, lipid composition- and CBD sequence-dependent basis behind the observed bioactivities of cCBD-LL37 and fCBD-LL37. We found that similar to LL37, cCBD-LL37 demonstrated pore formation mechanisms likely due to their similar charges, structural content and amphiphilicity. fCBD-LL37 demonstrated time-dependent, adsorption-based mechanism likely due to its anchoring aromatic residues, low charge, and low amphiphilicity. Knowledge gained from this study allowed mechanistic predictions of two newly designed hypothetical CBD-LL37 peptides. Results from this study contribute to a better understanding of a new class of antimicrobial, non-cytotoxic therapies based on collagen-tethered CBD-LL37, bringing it closer to clinical implementation in chronic wound applications and demonstrate the viability of biopolymer tethering as a platform toward using AMPs to quench the resistance crisis.

In the context of ongoing efforts toward C-aryl glycoside synthesis, a recently developed approach to form C-aryl glycals from 2-deoxysugar lactones was expanded to form novel substrates. This approach has been applied to the synthesis of various furyl glycals, allowing access to C-aryl glycals via a benzyne furan (4+2) cycloaddition methodology. The hydroboration-oxidation of said C-aryl glycals has allowed access to C(2)-oxygenated C-aryl glycosides via the benzyne cycloaddition approach. An approach to the total synthesis of 5-hydoxyaloin A is detailed, in which regioselective benzyne furan (4+2) cycloadditions were achieved via the use of a silicon tether. Two approaches to the anthrone core have been applied; one in which an unsymmetrically-substituted aryl ring is first constructed by means of a silicon tether, and one in which the unsymmetrically-substituted ring is formed last, also utilizing a silicon tether. The latter approach has allowed access to the anthrone core of 5-hydroxyaloin A, and only a final desulfurization remains in order to access the natural product. / text

Remotely Operated Vehicles (ROVs) provide access to underwater environments too deep and dangerous for commercial divers. A tether connects the ROV to a vessel on the surface, providing power and communication channels. During extended manoeuvres, hydrodynamic forces on the tether produce large tensions which hinder ROV manoeuvrability. The research presented in this thesis focuses on the design of new tether management strategies that alleviate the tether disturbance problem, and the implementation of a navigation suite for tracking the ROV position and velocity which are needed to close the loop on the tether management method. To improve the estimation of the ROV state, an Extended Kalman Filter (EKF) is developed.

Afin de réduire les émissions de gaz à effet de serre et le coût du transport maritime, l'utilisation des cerfs-volants comme système de propulsion auxiliaire des navires est prometteuse. Pour estimer les performances et l’opérabilité d’un navire tracté par cerf-volant, une modélisation dynamique du système est alors mise en oeuvre. Une modélisation analytique de cerf-volant est utilisée. Ce modèle néglige la masse du cerf-volant et suppose que les lignes sont droites et indéformables. Ces hypothèses conduisent à un modèle cinématique dépendant du coefficient de portance et de la finesse aérodynamique. Une évolution linéaire des coefficients aérodynamiques en fonction de la courbure de la trajectoire de vol est proposée. Par ailleurs, en développant un modèle quasi analytique de ligne, il est montré qu’à partir de 2 m.s-1 de vent relatif que l’hypothèse de ligne droite est raisonnable. En se basant sur un modèle de ligne, un critère analytique de vitesse de vent minimum permettant un vol quasi-statique est présenté. Dans le but de résoudre l’ensemble des termes d’interaction entre le cerf-volant et le navire, un modèle linéarisé de tenue à la mer temporelle est développé. Le produit de convolution de la réponse impulsionnelle du navire est calculé avec des systèmes d’états. Cependant comme celle-ci représente mal les mouvements horizontaux des navires, le modèle développé est alors couplé à un modèle de manoeuvrabilité. Pour étudier les interactions entre le cerf-volant et le navire un couplage monolithique et un couplage dissocié sont comparés. Le couplage dissocié néglige l’influence des mouvements du navire sur le vol du cerf-volant. En cas de mer calme, les résultats obtenus par les deux types de couplage sont très proches. En cas de houle régulière les mouvements du navire sont principalement causés par la houle. Le couplage monolithique montre qu’un réseau de sous-harmoniques basse fréquence apparait alors dans le spectre d’excitation du navire. La fréquence fondamentale des sous-harmoniques est donnée par la différence entre la fréquence de vague et la fréquence de l’harmonique la plus proche de l’excitation du kite. Quand cette différence est suffisamment petite, un phénomène d’accrochage apparait. Ce phénomène est bénéfique pour le cerf-volant et le navire quand le décalage des harmoniques d'excitation correspond à une augmentation. Par ailleurs, une étude de la stabilité de route montre qu'il est nécessaire de contrôler activement le safran. / In order to reduce greenhouse gas emissions and shipping costs, the use of kites as an auxiliary propulsion device for ships is promising. In order to estimate the performance and the operability of a kite-towed vessel, a dynamic modeling of the system is implemented. A classical kite modeling is used. This model neglects the mass of the kite and assumes straight and inelastic tethers. These assumptions lead to a kinematic model depending on the lift coefficient and the aerodynamic lift to drag ration angle. A linear evolution of these aerodynamic coefficients as a function of the curvature of the flight path is proposed. In addition, by developing a quasi-analytical line model, it is shown that from 2 m.s-1 of relative wind the straight tether assumption is reasonable. Based on the tether model, an analytical criterion assessing the minimum wind speed to enable a quasi-static kite flight is developed. To solve all the interaction terms between the kite and the ship, a time domain seakeeping model based on the linearized ship equation of motion assuming a potential flow is developed. The convolution product of the impulse response of the ship is computed with state-space systems. However, since horizontal ship motions are not well represented by such theories, a coupling with a maneuvering model is presented.Comparisons to experimental data tests show good agreements. To study the interactions between the kite and the ship, a monolithic coupling and a dissociated coupling are compared. The dissociated coupling neglects the influence of ship motions on the kite flight. In a calm water case, results obtained by the two types of coupling are very close. In regular waves, ship motions are dominated by the wave influence. Thus, with the monolithic coupling, a network of low frequency subharmonic appears in the kite excitation spectrum. The fundamental frequency of the subharmonic is given by the difference between the wave frequency and the frequency of the nearest kite excitation harmonic. When this difference is small enough, a lock-in phenomenon appears. This phenomenon is a benefit for the kite and the ship when the shift of the excitation harmonics corresponds to an increase. Furthermore, a course keeping stability study shows that the rudder needs to be actively controlled.

During vertebrate development, gastrulation leads to the formation of three distinct germlayers. In zebrafish a central process is the delamination and the ingression of single cells from a common ancestor tissue - that will lead to the formation of the germlayers. Several molecules have been identified to regulate this process but the precise cellular mechanisms are poorly understood. Differential adhesiveness, a concept first introduced by Steinberg over 40 years ago, has been proposed to represent a key phenomena by which single hypoblast cells separate from the epiblast to form the mesendoderm at later stages. In this work it is shown that differential adhesion among the germlayer progenitor cells alone cannot predict germlayer formation. It is a combination of several mechanical properties such as cell cortex tension, cell adhesion and membrane mechanical properties that influence the migratory behavior of the constituent cells.

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