One goal of this research was to characterize shoot tissue-level responses in loblolly pine to soil moisture limitation in combination with fertilization as well as to more severe soil moisture limitation. We found that neither fertilization alone, nor fertilization in combination with soil moisture limitation resulted in changes to shoot tissue water relations parameters classically characterized in drought response studies. More severe water limitation was necessary to elicit responses, and those responses had not been fully described previously. The more severe water limitation resulted in increased capacitance beyond turgor loss, increased relative water content at turgor loss, a more negative turgor loss point, an increased bulk modulus of elasticity, more negative osmotic potential at 100% relative water content, and an increased apoplastic water fraction. As there were indications of reduced water use and moisture stress in the absence of shoot level responses under less severe drought, such parameters are insufficient alone to characterize moisture stress in fertilized and in less severely water limited loblolly trees. Additionally, we sought a morphological or physiological explanation for the reduced transpiration and increased water use efficiency reported for fertilized trees in the Virginia Piedmont. Our characterizations of the responses of root distribution and hydraulics to limited soil moisture here complement existing research, which demonstrated changes to root distribution and hydraulics in response to fertilization. The responses we discovered in fertilized trees that accompanied reduced transpiration and increased water use efficiency that differed from responses to reduced soil moisture alone were primarily large decreases to shallow root presence. We found this to be readily quantified using measures of root length density. Decreases to whole-tree hydraulic conductivity were also shown to occur with fertilization and were shown not to occur in shoot tissue, suggesting limitation via rhizosphere or root xylem conductance. Our results support the supposition that fertilization narrows hydraulic safety margins and potentially predisposes loblolly trees to moisture stress, particularly prolonged, severe water limitation following fertilization. Finally, we tested the validity of throughfall exclusion for simulating reduced rainfall using a greenhouse 'split-pot' study, which applied spatially fixed heterogeneous soil moisture to young, well-watered loblolly pines. The 'split-pot' experiments demonstrated that spatially fixed soil moisture heterogeneity does not confound drought effects; needle area specific transpiration was not decreased, nor was water use efficiency increased. This supports the validity of inferences taken from drought simulation experiments with loblolly pine where throughfall exclusion troughs reduce soil moisture content in a consistent, spatially heterogeneous manner. / Doctor of Philosophy / We investigated various effects of soil moisture limitation alone, and in combination with common fertilization practices in loblolly pine production. Responses at the shoot and needle level to different levels of soil moisture limitation produced new findings concerning how tissues respond to more severe water limitation. A 30% decrease in throughfall precipitation alone, or in combination with fertilization did not elicit drought related shoot tissue responses despite the presence of other indications of moisture stress and reduced water use. We also sought to explain why fertilized trees experiencing water limitation had environmental sensitivities that were different from unfertilized tree receiving ambient rainfall amounts or from trees only experiencing water limitation without fertilization. We found that changes to shallow root presence, especially root length density, accompanied the different patterns of environmental sensitivity and water use. Also, the water conducting ability of roots changed unevenly in soil with uneven moisture levels. The ability of roots to resist loss of conductivity to water did not change unevenly in the same way. We did another set of experiments to determine if using impervious troughs to catch rain is a valid approach to reducing soil moisture for the purpose of testing how loblolly responds to water limitation. These throughfall exclusion troughs create uneven soil moisture reduction, which can have effects on plant water use that are separate from water limitation alone. We found that in well-watered young trees, uneven soil moisture alone did not produce responses that could be confused with the effects of water limitation. This finding indirectly validates the use of throughfall exclusion troughs to simulate reduced rainfall.

n fall 2022, disdrometer measurements were carried out at the MyDiv experiment in Bad Lauchstadt for six weeks. One optical Thies laser disdrometer was set up in the open field and a second one in a series of monoculture plots of tree communities. After several precipitation events, the disdrometer below canopy was moved to a different monoculture plot. The measurements aimed to investigate precipitation throughfall characteristics under different tree species compared to the disdrometer in open land. This paper first gives a brief overview about the measurement campaign and the instrumentation. Afterward, the data quality and filtering of the data are explained. This is followed by the data evaluation, in which both, the number of hydrometeors, and the amount and intensity of precipitation under the tree species are analyzed in comparison to the open field disdrometer. In addition, the precipitation amount is analyzed across tree species of different foliage density. / Im Herbst 2022 wurden auf dem Gelände des MyDiv Experiments in Bad Lauchstadt über sechs Wochen hinweg Distrometermessungen durchgeführt. Dabei wurde ein optisches Thies Laser Distrometer unter freiem Himmel und ein zweites in eine Reihe von Monokulturparzellen gestellt. Nach einigen Niederschlagsevents wurde das Distrometer unter dem Kronendach in eine andere Monokulturparzelle versetzt. Das Ziel der Messungen war dabei, die Niederschlagscharakteristika unter verschiedenen Baumarten im Vergleich zum Distrometer zu untersuchen. In dieser Arbeit wird zunachst ein kurzer Überblick über die Messkampagne und das Messgerät gegeben. Im Anschluss wird die Datenqualitat und Filterung der Daten erläutert. Daraufhin folgt die Datenauswertung, in der sowohl die Anzahl der Hydrometeore als auch die Menge und Intensität der Niederschlagsereignisse unter den Baumarten im Vergleich zum freistehenden Distrometer analysiert wird. Darüber hinaus wird weiterführend die Niederschlagsmenge auch zwischen Messungen unter Baumarten verschiedener Belaubungsdichte untersucht.

Os estudos sobre o clima vêm adquirindo grande importância na área ambiental, principalmente em relação à qualidade do ar (poluição atmosférica), ao conforto térmico (ilhas de calor) e aos impactos pluviais (geradores de inundações urbanas). Nesses últimos, as chuvas causam muitos transtornos para a população e órgãos públicos competentes, devido aos eventos extremos, que estão acontecendo em toda parte do mundo. Esses eventos ocorrem desde uma chuva com muita intensidade, em curto espaço de tempo, até seca prolongada em certos lugares. O fato é preocupante e mesmo com tantas controvérsias sobre o assunto, é necessário um manejo, principalmente no sistema hidrológico, que se adeqüe a cada situação e que os prejuízos causados pelas enchentes não se tornem mais devastadores do que atualmente. Como a arborização pode amenizar tais efeitos impactantes, nesse propósito, foi realizado um estudo no campus da ESALQ/USP, durante o período de janeiro a fevereiro de 2007, para medir a interceptação da água da chuva pelas copas das árvores e verificar como algumas espécies poderiam minimizar enchentes em áreas urbanas. O processo de interceptação da água da chuva foi analisado em duas espécies muito comumente usadas na arborização urbana, visando obter estimativas da quantidade de interceptação (I) ou perda por interceptação, precipitação interna (PI), escoamento pelo tronco (Et) e precipitação total (PT). As espécies escolhidas foram Caesalpinia pluviosa DC. (Fabaceae: Caesalpinoideae), ou sibipiruna, e Tipuana tipu O. Kuntze (Fabaceae: Faboideae), ou tipuana, com mais de 50 anos de idade, sendo três indivíduos isolados e três em grupo de cada espécie. As medições foram feitas por meio de coleta da água não interceptada tanto nas extremidades como no centro da projeção das copas. A PI apresentou alta correlação com a quantidade de PT no centro da copa em tipuana, e na extremidade da copa em sibipiruna. O Et e a I tiveram baixa correlação com a quantidade de PT nas duas espécies. As médias de interceptação foram maiores na extremidade da copa nos indivíduos de sibipiruna, com 60,6%, e no centro da tipuana, com 59,4%. Para precipitações variando de 0 a 2,5 mm, a interceptação média foi de 78,7%, em um indivíduo de sibipiruna, na extremidade da copa e, de 80,9% nos indivíduos de tipuana, em grupo, no centro da copa. Enquanto que, para precipitação de 2,5 a 5,0 mm, a interceptação foi de 53,6% para tipuana em grupo, na extremidade e, de 51,9% na tipuana isolada, no centro da copa. A tipuana apresentou maior porcentagem média de interceptação no centro da copa, principalmente em eventos com mais de 2 horas de duração e com precipitação maior que 20,0 mm, tanto em indivíduos isolados como em grupo. Portanto, as duas espécies demonstraram grande potencial para serem plantadas no ambiente urbano, com capacidade de contribuírem para possíveis reduções das enchentes nas cidades, principalmente a tipuana que mostrou mais predisposta a essa ação. / Climate studies are receiving more attention in the environmental field, especially concerning air quality (atmosphere pollution), thermal comfort (heat islands) and pluvial impacts (urban flood generators). In the latter, the rain causes disturbance of the population and the competent public organ, due the extreme events that are increasing in the whole world. Theses events can come as a precipitation of very intensity in a short period of time, as well as a prolonged drought in a given location. The issue is worrisome and controversial, however a managerial solution is necessary and urgent, particularly about the hydrologic system for each situation so that the losses caused by floods do not become more devastating than now. With these purpose in mind, a study was carried out at the campus of \"Luiz de Queiroz\" College of Agriculture (University of Sao Paulo), Piracicaba, Brazil, from January to February 2007, in order to measure the rainfall interception by the crows of urban trees and estimate the rainfall interception that they provide to potentially minimize urban flood events. The rainfall interception was evaluated in the two commonly used species in forest urban spaces by the measurement of interception (I) or interception losses, throughfall (Th), stemflow (St) and gross precipitation (Pg). The chosen trees were more than 50 year-old specimens of Caesalpinia pluviosa DC. (Fabaceae: Caesalpinoideae) or sibipiruna, and Tipuana tipu O. Kuntze (Fabaceae: Faboideae) or tipuana, both displayed as three isolated individuals and three grouped individuals. Measurements were carried out by collecting the water falling by the edges and in the centre of the canopies. A high correlation of Th with Pg was observed on the centre of the crow of tipuana and by the edges of sibipiruna. St and I had low correlation with Pg for both species. . The rain interception mean was higher by the edges of the crow of sibipiruna individuals, 60.6%, and in the centre of the tipuana crow, 59.40%.The mean of the rainfall interception was 78.73% for one isolated sibipiruna by the edge of the crow and 80.94% for grouped tipuanas in centre of the crow, when the total precipitation ranged between 0 and 2.5 mm. For total precipitation values between 2.5 and 5.0 mm, the interception mean was 53.60% for grouped tipuanas by the edge and 51.89% in isolated tipuanas in the crow center. Thus, both species show a great potential to be planted in urban environmental, especially for the tipuana species, which presented the capacity of contributing better to reduce or minimize floods in cities.

Conselho Nacional de Desenvolvimento Científico e Tecnológico / ABSTRACT: In regions of natural or planted forests, rainfall interception by vegetation can be an important variable in the hydrological cycle and should not be neglected. This research analyzes the process of interception in different forest types (native and planted) in the region of Santa Maria - RS, at the head of Vacacaí Mirim. The variables Rainfall (R), stemflow (Sf), and throughfall (Tf) were monitored in the period from Dec/09 to Fev/11. Rainfall was monitored in two tipping bucket rain gauges and meteorological station installed in the subbasin on July 14th, 2010. Throughfall was monitored in three plots (areas I, II and III) with dimensions of 12 m x 50 m, 21 m x 12 m and 10 m x 20 m, respectively. The sampling points were installed 40 or 60 cm from the ground and spaced 1 m in straight line. In area I (native forest), 101 stakes were allocated to 40 instruments; where 20 collectors remained in fixed locations and 20 were rotated randomly. In the areas II (native forest) and III (eucalyptus) 60 sampling points were allocated to 20 collectors; at each new collection they were placed one sampling point ahead the former. Stemflow was based on values suggested in literature and by monitoring the flow in three selected trees within each plot, and the interception was calculated as the difference between precipitation and the sum of throughfall and stemflow. The results showed that interception is a key component of the hydrological cycle and, in general, underestimated by the hydrological models.The highest interception value was checked for native forest and the largest stemflow for eucalyptus. The stemflow was higher than the values observed by other authors, revealing that this variable cannot be ruled out in the study of interception. On the other hand, the ranges of variation of throughfall and interception were close to those reported in other studies. The results seem also to suggest that the depth parameter adopted for the calculation of interception using the leaf area index is underestimated and should be 0.4. Statistical analysis of the number of collectors used and necessary to ensure error of 5 mm of throughfall in relation to its average and 90% of confidence, showed that for vegetation such as Eucalyptus, the use of 20 rotating collectors were sufficient in 95.45% of the collections; which may be a reference to works in which there is a pattern of similarity of species. For native forest we observed that when the vegetation has a low density and the similar pattern of canopy, stem diameter and height, the use of 20 rotating collectors would be enough to ensure statistical requirements set out in 71.43% of the samples monitored. When the native vegetation is dense, with standard range of height, stem diameter and crown area, we observed the need for a higher number of collectors equipment of throughfall. The use of 40 collectors (20 fixed and 20 rotational) assured that the statistical requirements stipulated were guaranteed in 60.98% of the samples monitored. / RESUMO: Nas regiões de floretas naturais ou plantadas, a interceptação da precipitação pela vegetação pode ser uma importante variável do ciclo hidrológico, não devendo ser negligenciada. Esta pesquisa analisou o processo de interceptação em diferentes formações florestais (nativa e plantada) na região de Santa Maria - RS, na cabeceira do Vacacaí Mirim. As variáveis monitoradas no período de dez/09 a fev/11 foram: precipitação inicdente (P), escoamento pelo tronco (Esct) e precipitação interna (Pi). A precipitação incidente foi monitorada em dois pluviômetros tipo cubas-basculantes e pela estação climatológica instalada na sub-bacia em 14/7/2010. A precipitação interna foi monitorada em três parcelas (área I, II e III) com dimensões de 12 m x 50 m, 21 m x 12 m e 10 m x 20 m, respectivamente. As estacas foram instaladas a 40 ou 60 cm do solo, sendo espaçadas de 1 m e em linha reta. Na área I (mata nativa), foram alocadas 101 estacas para disposição de 40 instrumentos, sendo que 20 permaneceram fixos e 20 foram rotacionados. Já nas áreas II (mata nativa) e III (eucalipto) foram alocadas 60 estacas para disposição de 20 coletores, sendo que a cada nova coleta eles eram realocados uma estaca a frente da anterior. O escoamento pelo tronco foi determinado com base em valores sugeridos em literatura e por meio de monitoramento do escoamento em três árvores escolhidas dentro de cada parcela e a interceptação foi calculada pela diferença entre P e a soma de Pi e Esct. Os resultados mostraram que a interceptação é uma componente fundamental do ciclo hidrológico e, em geral, subestimada pelos modelos hidrológicos. O maior valor de interceptação foi verificado para mata nativa e o maior Esct para eucalipto. O escoamento pelo tronco foi superior aos valores verificados por outros autores, revelando que essa variável não pode ser descartada no estudo da interceptação. Já as faixas de variação de precipitação interna e da interceptação ficaram próximas aquelas registradas em outros estudos. Os resultados parecem sugerir, ainda, que o parâmetro de lâmina adotado para o cálculo de interceptação com utilização do índice de área foliar está subestimado e deveria ser 0,4. A análise estatística do número de aparelhos utilizados e necessários para garantir 5 mm de erro de Pi em relação a sua média e 90% de confiança, mostrou que para vegetação do tipo eucalipto, o uso de 20 instrumentos rotacionáveis seriam suficientes em 95,45% das coletas realizadas. O que pode ser uma referência para trabalhos em que existe um padrão de semelhança de espécies. Já para mata nativa observou-se que, quando a vegetação apresenta baixa densidade e mesmo padrão de copa, diâmetro do caule e altura, o uso de 20 instrumentos rotacionáveis seria suficiente para garantir os requisitos estatísticos estipulados em 71,43% das coletas monitoradas. Quando a vegetação nativa é densa, com padrão variado de altura, diâmetro do caule e área da copa, percebeu-se a necessidade de um número mais elevado de equipamentos coletores de Pi. O uso de 40 instrumentos, (20 aparelhos rotacionáveis e 20 fixos), garantiu que em 60,98% das coletas monitoradas os requisitos estatísticos estipulados fossem assegurados.

Os estudos sobre o clima vêm adquirindo grande importância na área ambiental, principalmente em relação à qualidade do ar (poluição atmosférica), ao conforto térmico (ilhas de calor) e aos impactos pluviais (geradores de inundações urbanas). Nesses últimos, as chuvas causam muitos transtornos para a população e órgãos públicos competentes, devido aos eventos extremos, que estão acontecendo em toda parte do mundo. Esses eventos ocorrem desde uma chuva com muita intensidade, em curto espaço de tempo, até seca prolongada em certos lugares. O fato é preocupante e mesmo com tantas controvérsias sobre o assunto, é necessário um manejo, principalmente no sistema hidrológico, que se adeqüe a cada situação e que os prejuízos causados pelas enchentes não se tornem mais devastadores do que atualmente. Como a arborização pode amenizar tais efeitos impactantes, nesse propósito, foi realizado um estudo no campus da ESALQ/USP, durante o período de janeiro a fevereiro de 2007, para medir a interceptação da água da chuva pelas copas das árvores e verificar como algumas espécies poderiam minimizar enchentes em áreas urbanas. O processo de interceptação da água da chuva foi analisado em duas espécies muito comumente usadas na arborização urbana, visando obter estimativas da quantidade de interceptação (I) ou perda por interceptação, precipitação interna (PI), escoamento pelo tronco (Et) e precipitação total (PT). As espécies escolhidas foram Caesalpinia pluviosa DC. (Fabaceae: Caesalpinoideae), ou sibipiruna, e Tipuana tipu O. Kuntze (Fabaceae: Faboideae), ou tipuana, com mais de 50 anos de idade, sendo três indivíduos isolados e três em grupo de cada espécie. As medições foram feitas por meio de coleta da água não interceptada tanto nas extremidades como no centro da projeção das copas. A PI apresentou alta correlação com a quantidade de PT no centro da copa em tipuana, e na extremidade da copa em sibipiruna. O Et e a I tiveram baixa correlação com a quantidade de PT nas duas espécies. As médias de interceptação foram maiores na extremidade da copa nos indivíduos de sibipiruna, com 60,6%, e no centro da tipuana, com 59,4%. Para precipitações variando de 0 a 2,5 mm, a interceptação média foi de 78,7%, em um indivíduo de sibipiruna, na extremidade da copa e, de 80,9% nos indivíduos de tipuana, em grupo, no centro da copa. Enquanto que, para precipitação de 2,5 a 5,0 mm, a interceptação foi de 53,6% para tipuana em grupo, na extremidade e, de 51,9% na tipuana isolada, no centro da copa. A tipuana apresentou maior porcentagem média de interceptação no centro da copa, principalmente em eventos com mais de 2 horas de duração e com precipitação maior que 20,0 mm, tanto em indivíduos isolados como em grupo. Portanto, as duas espécies demonstraram grande potencial para serem plantadas no ambiente urbano, com capacidade de contribuírem para possíveis reduções das enchentes nas cidades, principalmente a tipuana que mostrou mais predisposta a essa ação. / Climate studies are receiving more attention in the environmental field, especially concerning air quality (atmosphere pollution), thermal comfort (heat islands) and pluvial impacts (urban flood generators). In the latter, the rain causes disturbance of the population and the competent public organ, due the extreme events that are increasing in the whole world. Theses events can come as a precipitation of very intensity in a short period of time, as well as a prolonged drought in a given location. The issue is worrisome and controversial, however a managerial solution is necessary and urgent, particularly about the hydrologic system for each situation so that the losses caused by floods do not become more devastating than now. With these purpose in mind, a study was carried out at the campus of \"Luiz de Queiroz\" College of Agriculture (University of Sao Paulo), Piracicaba, Brazil, from January to February 2007, in order to measure the rainfall interception by the crows of urban trees and estimate the rainfall interception that they provide to potentially minimize urban flood events. The rainfall interception was evaluated in the two commonly used species in forest urban spaces by the measurement of interception (I) or interception losses, throughfall (Th), stemflow (St) and gross precipitation (Pg). The chosen trees were more than 50 year-old specimens of Caesalpinia pluviosa DC. (Fabaceae: Caesalpinoideae) or sibipiruna, and Tipuana tipu O. Kuntze (Fabaceae: Faboideae) or tipuana, both displayed as three isolated individuals and three grouped individuals. Measurements were carried out by collecting the water falling by the edges and in the centre of the canopies. A high correlation of Th with Pg was observed on the centre of the crow of tipuana and by the edges of sibipiruna. St and I had low correlation with Pg for both species. . The rain interception mean was higher by the edges of the crow of sibipiruna individuals, 60.6%, and in the centre of the tipuana crow, 59.40%.The mean of the rainfall interception was 78.73% for one isolated sibipiruna by the edge of the crow and 80.94% for grouped tipuanas in centre of the crow, when the total precipitation ranged between 0 and 2.5 mm. For total precipitation values between 2.5 and 5.0 mm, the interception mean was 53.60% for grouped tipuanas by the edge and 51.89% in isolated tipuanas in the crow center. Thus, both species show a great potential to be planted in urban environmental, especially for the tipuana species, which presented the capacity of contributing better to reduce or minimize floods in cities.

Les hydrocarbures aromatiques polycycliques (HAP) sont des polluants organiques persistants (POP) émis par les activités humaines suite à la combustion incomplète de la matière organique (industrie, chauffage, trafic routier...). En raison de leur faible taux de dégradation, de leur toxicité, de leur capacité à être transportés sur de longues distances et de leur bioaccumulation dans la matière organique, les HAP font l'objet d'une surveillance atmosphérique (directive cadre européenne 96/62/CE du 27 septembre 1996). Les bryophytes, plus communément appelées mousses, sont utilisées depuis une trentaine d'années pour la biosurveillance de la qualité de l'air. Leurs caractéristiques biologiques font de ces plantes terrestres d'excellents bioaccumulateurs des dépôts de polluants atmosphériques. Cependant, elles ont été peu utilisées pour la biosurveillance des HAP et pour des échelles spatiales et temporelles très limitées. Dans ce contexte, la thèse a pour objectif de valider l'utilisation de bryophytes comme outils pour la biosurveillance des retombées atmosphériques de HAP à différentes échelles spatio-temporelles. La première phase de ce travail a consisté en un développement analytique afin de valider l'analyse des HAP par chromatographie liquide associée à une détection par fluorescence (CLHP-FLD), l'extraction liquide-liquide des HAP contenus dans les dépôts totaux et l'extraction par liquide pressurisé (PLE) des HAP contenus dans les mousses. Cette dernière a été optimisée, grâce à la réalisation d'un plan d'expériences, et a été complétée par des tests de purification par extraction sur phase solide (SPE). Des études de terrain ont ensuite été réalisées afin d'évaluer les tendances temporelles à l'échelle saisonnière, annuelle et historique, ainsi que les tendances spatiales à l'échelle locale et régionale, des teneurs en HAP dans les mousses. Une étude menée sur des mousses d'herbier prélevées dans le Parc Naturel de Bertiz (Navarre, Espagne) entre 1879 et 2007 a permis d'identifier les sources historiques d'émission des HAP. Les bryophytes apparaissent comme de bons « témoins » de la contamination atmosphérique du passé. Une étude annuelle (2010-2011) de biosurveillance active menée à Bertiz a permis ensuite d'observer des tendances saisonnières des teneurs en HAP dans les mousses Hylocomium splendens (Hedw.) Schimp., liées aux variations des émissions atmosphériques et des conditions météorologiques, en particulier des précipitations. De plus, l'étude annuelle a révélé un fractionnement lors de l'accumulation par les mousses des HAP contenus dans les dépôts totaux, qui résulte d'une affinité des HAP pour les dépôts liée à leur solubilité et d'une affinité des HAP pour le végétal liée à leur propriété lipophile (KOW). Ainsi, le suivi des flux atmosphériques de HAP dans les dépôts totaux ne permet pas de prévoir a priori les teneurs de HAP dans les écosystèmes. Enfin, une analyse multivariée de la distribution spatiale des teneurs en HAP dans l'espèce Hypnum cupressiforme Hedw. prélevée en Espagne (Navarre), France (Île-de-France) et Suisse (Plateau Suisse et région de Bâle) a permis de caractériser les sources de contamination atmosphériques des régions étudiées et l'influence de paramètres environnementaux tels que l'altitude et la pluviométrie. / Polycyclic aromatic hydrocarbons (PAHs) are persistent organic pollutants (POPs) emitted by human activities during incomplete combustion of organic matter (industry, domestic heating, road traffic…). Due to their low degradation rates, toxicity, long-range transport and bioaccumulation in organic matter, PAHs are monitored in the atmosphere (Council Directive 96/62/EC of 27 September 1996). Bryophytes, more commonly called mosses, have been used during the past three decades for biomonitoring air quality. Because of their biological characteristics, these terrestrial plants are excellent bioaccumulators of airborne pollutants. However, few studies concern PAH biomonitoring and they are usually restricted to studies limited in time and space. In this context, the thesis aims to validate bryophytes as tools for biomonitoring atmospheric deposition of PAHs on various space-time scales. The first step of this work consisted in developing the analytical procedures in order to validate PAH analysis by liquid chromatography associated with fluorescence detection (HPLC-FLD), liquid-liquid extraction of PAHs from bulk deposition and pressurized liquid extraction (PLE) of PAHs from mosses. The latter was optimized with a factorial design and was completed by purification tests with solid phase extraction (SPE). Field studies were then carried out to evaluate temporal trends of PAH levels in mosses at seasonal, annual and historical scales, as well as spatial trends, at local and regional scales. A study undertaken on herbarium mosses sampled in Bertiz Natural Park (Navarra, Spain) between 1879 and 2007 leaded to the identification of PAH historical emission sources. Bryophytes appear as excellent “witnesses” of the past atmospheric contamination. An annual biomonitoring study (2010-2011) at Bertiz showed seasonal trends of PAH content in mosses Hylocomium splendens (Hedw.) Schimp., which are related to variations of PAH emissions and weather conditions, particularly precipitations. Moreover, the annual study revealed a fractionation of PAHs while they are transferred from total deposition to the mosses, as the affinity of PAHs for deposition samples was related with their solubility and their affinity for the plant was related to their lipophilic property (KOW). Thus, monitoring PAH fluxes in total deposition does not give an a priori estimation of PAH contents in the ecosystems. Finally, multivariate analysis of the spatial distribution of PAHs in the species Hypnum cupressiforme Hedw. sampled in Spain (Navarra), France (Île-de-France) and Switzerland (Swiss Plateau and Basel area) leaded to the characterization of the atmospheric contamination sources in the different areas and the influence of environmental parameters such as elevation and pluviometry.

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