No description available.

Studies of biodegradation in lignins by basidiomycetes yeasts show the conversion of lignin in various degradation products among which vanillin, a valuable substance, suggested to be a strong inhibitor of both fermentation and growth of yeasts, stands. Sporidiobolales yeasts used in these experiments were aimed to be identified by their highly conserved ITS region as well as studied in vanillinsupplemented medium through, vanillin-supplemented plates, TLC and Neubauer’s chamber to find out which, among the several isolates tested, were the most resistant ones, understand how they take up vanillin and how their growth is affected by the presence of the phenolic compound. Two strains were identified as Rhodotorula babjevae. One of them, L4, together with LS22, Rhodosporidium kratochvilovae, could withstand and biodegrade high concentrations of vanillin, producing biodegradation products with Rf values similar to the ones know for vanillic acid and vanillyl alcohol. Better growth in medium supplemented with small doses of vanillin was found, as well as disparity among the same species and their metabolic features, therefore, herbicides resistance was suggested as a reason for strains divergence. Further morphological-species comparison could also describe if there exist a relation between them. / Estudios sobre la biodegradación de ligninas por levaduras basidiomicetes muestran la conversión de lignina en distintos productos de degradación, entre los cuales se encuentra la vainillina, un fuerte inhibidor de la fermentación y el crecimiento de levaduras. Las levaduras Sporidiobolales utilizadas en estos experimentos han intentado ser identificadas a través de la región ETI, muy conservada, además de estudiadas en medios suplementados con vainillina mediante placas suplementadas con vainillina, CCF y cámara de Neubauer para averiguar cuáles son las cepas más resistentes, entender cómo metabolizan la vainillina y cómo su crecimiento se ve afectado por la presencia de dicho compuesto. Dos cepas fueron identificadas como Rhodotorula babjevae. Una de ellas, L4, junto con con la cepa LS22, Rhodosporidium kratochvilovae, pudieron soportar y biodegradar elevadas concentraciones de vainillina, originando productos de biodegradación con valores de Rf similares a los del ácido vanílico y alcohol vanílico previamente conocidos. Se encontró un crecimiento mejor en medios suplementados con pequeñas dosis de vainillina además de una disparidad entre mismas especies y sus características metabólicas, así, herbicidas han sido sugeridos como una posible causa en dicha divergencia. Una futura comparación morfología-especie podrá describir si existe relación entre ambos.

Plant-feeding mites represent major pests in agriculture that are of importance to crops world-wide, as large populations of mites reduce the quality and quantity of yields. Alternatives to the use of pesticides are needed due to their negative effects and bio-control agents (predatory mites) remain advantages as they suppress spider mites and other plant pests. This study aims to determine species status of plant-feeding and predatory mites on plants of the family Solanaceae and to apply morphological and molecular data to determine phylogenetic relationships among economically important Phytoseiidae, Stigmaeidae and Tetranychidae. The material for this study was collected through plant beating and specimens were preserved in 75% and 96% ethanol respectively and mounted in Heinz’s PVA medium on microscope slides. A survey was conducted during peak seasons to provide enough samples of pest and predatory species. Morphological analysis was performed and initial results indicate that 94% of the species identified were parasitic and 6% were predatory, which led to a predator:prey ratio of 1:17, where Tetranychus evansi Baker & Pritchard had the highest frequency of appearance. A modified Qiagen DNeasy tissue kit extraction protocol was used and Polymerase Chain Reaction was performed to amplify ribosomal ITS and mitochondrial COI gene fragments. The nucleotide sequence of a 700-bp fragment for ITS was determined by direct sequencing as well as for a 700-bp and 800-bp fragments for COI. The resulting data included 4 isolates that corresponded morphologically and molecularly with Phytoseiidae and 10 with Stigmaeidae. The phylogenetic trees agreed with the morphological data. For species that lack morphological descriptions in GenBank and are not placed within expected clades, one has to accept the possibility of miss identification and highlights the need to combine morphological and molecular approaches to guarantee solid species diagnosis. Ultimately, Solanaceae contain various parasitic mites, but predators seem low in numbers. This could be problamatic in finding effective bio-control agents. / MSc (Zoology), North-West University, Potchefstroom Campus, 2015

Plant-feeding mites represent major pests in agriculture that are of importance to crops world-wide, as large populations of mites reduce the quality and quantity of yields. Alternatives to the use of pesticides are needed due to their negative effects and bio-control agents (predatory mites) remain advantages as they suppress spider mites and other plant pests. This study aims to determine species status of plant-feeding and predatory mites on plants of the family Solanaceae and to apply morphological and molecular data to determine phylogenetic relationships among economically important Phytoseiidae, Stigmaeidae and Tetranychidae. The material for this study was collected through plant beating and specimens were preserved in 75% and 96% ethanol respectively and mounted in Heinz’s PVA medium on microscope slides. A survey was conducted during peak seasons to provide enough samples of pest and predatory species. Morphological analysis was performed and initial results indicate that 94% of the species identified were parasitic and 6% were predatory, which led to a predator:prey ratio of 1:17, where Tetranychus evansi Baker & Pritchard had the highest frequency of appearance. A modified Qiagen DNeasy tissue kit extraction protocol was used and Polymerase Chain Reaction was performed to amplify ribosomal ITS and mitochondrial COI gene fragments. The nucleotide sequence of a 700-bp fragment for ITS was determined by direct sequencing as well as for a 700-bp and 800-bp fragments for COI. The resulting data included 4 isolates that corresponded morphologically and molecularly with Phytoseiidae and 10 with Stigmaeidae. The phylogenetic trees agreed with the morphological data. For species that lack morphological descriptions in GenBank and are not placed within expected clades, one has to accept the possibility of miss identification and highlights the need to combine morphological and molecular approaches to guarantee solid species diagnosis. Ultimately, Solanaceae contain various parasitic mites, but predators seem low in numbers. This could be problamatic in finding effective bio-control agents. / MSc (Zoology), North-West University, Potchefstroom Campus, 2015

Thèse numérisée par la Division de la gestion de documents et des archives de l'Université de Montréal.

Dead wood plays an important role in forest ecosystems in the context of C dynamics, nutrient cycling, forest regeneration and biodiversity. Decaying wood sustains biodiversity by providing habitats and energy for fungi, bacteria, invertebrates, and many other organisms. Dead wood is resistant to decomposition and its decay is driven mainly by filamentous fungi. Community structure of wood- inhabiting fungi changes during decomposition, but the relationship between substrate quality and decomposer community is still poorly understood. This work studied fungal community composition with respect to tree species, stage of decay, volume and physico-chemical properties (such as pH, carbon and nitrogen content) of dead wood. Fungi were identified using next generation sequencing approaches - 454-pyrosequencing and Illumina MiSeq sequencing. Tree species, volume of dead wood (branches x logs) and stage of decay were the main variables affecting fungal community composition. Higher enzyme activities and content of fungal biomass indicate faster colonization of small branches than tree trunks by fungi. Fungal community composition, wood chemical properties and enzyme activities changed during decomposition. Both content of nitrogen and fungal biomass increased during decomposition. Enzyme activites peaked...

Eleven species are distinguished in the genus Oxythyrea Mulsant, 1842 (Coleoptera: Scarabaeidae) nowadays. They are not divided into subspecies. Diversity of the genus is concentrated in the Mediterranean and Oxythyrea funesta (Poda, 1761) inhabit a wide area in the western Palearctic Region. It was observed in last decades, that O. funesta retreated from central Europe to south and then recolonized it back including new areas in northern regions. Master thesis is focused on resolving population structure of O. funesta and partial phylogeny of the genus Oxythyrea using molecular genetic methods. 145 individuals of O. funesta and 15 individuals of five other species of the genus Oxythyrea appear in analysis. We acquired sequences of mitochondrial genes cytochrome oxidase I (807 bp), cytochrome b (381 bp) and nuclear gene internal transcribed spacer 1 (946 bp) from these specimens. The results of phylogenetic analysis confirmed so far the only one existing interpretation of relationships within the genus Oxythyrea based on morphological data. We also confirmed complicated relationships between O. funesta and O. pantherina, which also appear in the historical development of their taxonomy. We detected different genetic lineage in Sicily, southern Italy and Tunisia using phylogenetic trees and haplotype...

Sugawara no Michizane était un lettré du 9e siècle qui fut élevé au rang de kami et qui inspira un culte qui demeure encore extrêmement vivace de nos jours. Le personnage historique et sa forme divinisée inspirèrent quantité d’histoires qui furent, entre autres raisons, créées pour justifier l’édification de sanctuaires voués à son culte, expliquer l’origine d’un fait religieux local ou d’un toponyme. Dans ce travail, nous nous proposons d'étudier les récits fictifs qui mettent en scène le personnage historique de Sugawara no Michizane et d’aborder le sujet sous trois angles différents. La première partie de notre étude consistera en l’établissement d’une énumération de ces légendes au moyen d’un large appareil de documents (récits de fondation, monographies, journaux de voyage…) qui nous permettra d’identifier leurs particularités, leur répartition dans l’espace et dans la vie de Sugawara no Michizane. Cette liste permettra de faire ressortir l’existence d’un certain nombre de « valeurs constantes » que l’on retrouve en fait non seulement dans les histoires de Sugawara no Michizane mais aussi dans celles qui mettent en scène d’autres personnages historiques. La seconde approche de ces légendes consistera à étudier précisément ces « valeurs constantes » et à tenter d’en déterminer l’origine. La troisième et dernière approche des légendes aura pour but d’étudier leurs « valeurs variables » et de voir ce qu’elles peuvent, à leur tour, nous apprendre sur leurs auteurs ainsi que les circonstances de leur création. / Sugawara no Michizane was a 9th century scholar who was turned into a god and inspired a cult still popular nowadays. His human and divine forms both inspired countless legends which were told, amongst other reasons, in order to justify the building of shrines devoted to his cult, and to explain the origin of local customs and places’ names. This dissertation will focus on the legends built around the historical figure of Sugawara no Michizane and analyze them under three different perspectives. The first perspective consists of an enumeration of legends using a large array of documents such as foundation stories, monographs and travelers’ journals. This is the first time such a list has been compiled. This list enables us to identify particularities and distributions in both space and span of Sugawara no Michizane’s life. This list also allows us to establish the existence of a certain number of “constant patterns” which not only appear in Sugawara no Michizane’s stories but also in stories involving other historical figures. The second perspective of the study examines these “constant patterns” and suggests possibilities for their possible origin. The third and last perspective of our study will deal with the so-called “variable patterns” of the stories and will suggest who created these stories and the reasons for their creation.

Thèse numérisée par la Division de la gestion de documents et des archives de l'Université de Montréal

Dealing with low-resource languages is a challenging task, because of the absence of sufficient data to train machine-learning models to make predictions on these languages. One way to deal with this problem is to use data from higher-resource languages, which enables the transfer of learning from these languages to the low-resource target ones. The present study focuses on dependency parsing and part-of-speech tagging of low-resource languages belonging to the spoken genre, i.e., languages whose treebank data is transcribed speech. These are the following: Beja, Chukchi, Komi-Zyrian, Frisian-Dutch, and Cantonese. Our approach involves investigating different types of transfer languages, employing MACHAMP, a state-of-the-art parser and tagger that uses contextualized word embeddings, mBERT, and XLM-R in particular. The main idea is to explore how the genre, the language similarity, none of the two, or the combination of those affect the model performance in the aforementioned downstream tasks for our selected target treebanks. Our findings suggest that in order to capture speech-specific dependency relations, we need to incorporate at least a few genre-matching source data, while language similarity-matching source data are a better candidate when the task at hand is part-of-speech tagging. We also explore the impact of multi-task learning in one of our proposed methods, but we observe minor differences in the model performance.