Response times have been used to explore cognitive and perceptual processes since 1850 (Donders, 1868). The technique has primarily been applied to humans, birds, and terrestrial mammals. Results from two studies are presented here that examine response times in bottlenose dolphins (Tursiops truncatus) and white whales (Delphinapterus leucas). One study concerned response times to stimuli well above the threshold of perceptibility of a stimulus, and the other concerned response times to stimuli near threshold. Two white whales (Delphinapterus leucas) and five Atlantic bottlenose dolphins (Tursiops truncatus) were presented stimuli well above threshold. The stimuli varied in type (tone versus pulse), amplitude, duration, and frequency. The average response time for bottlenose dolphins was 231.9 ms. The average response time for white whales was 584.1 ms. There was considerable variation between subjects within a species, but the difference between species was also found to be significant. In general, response times decreased with increasing stimulus amplitude. The effect of duration and frequency on response time was unclear. Two white whales (Delphinapterus leucas) and four Atlantic bottlenose dolphins (Tursiops truncatus) were given audiometric tests to determine masked hearing thresholds in open waters of San Diego Bay (Ridgway et al., 1997). Animals were tested at six frequencies over a range from 400 Hz to 30 kHz using pure tones. Hearing thresholds varied from 87.5 dB to 125.5 dB depending on the frequency, masking noise intensity and individual animal. At threshold, median response time across frequencies within each animal varied by about 150 ms. The two white whales responded significantly slower (∼670 msec, p<0.0001) than the four dolphins (∼410 msec). As in terrestrial animals, reaction time became shorter as stimulus amplitude increased (Wells, 1913; Stebbins, 1966). Across the two studies, the dolphins as a group were faster in the abovethreshold study than in the nearthreshold study. White whales had longer response times than bottlenose dolphins in both studies. Analysis of response time with an allometric relation based on weight shows that the difference in weight can explain a significant part of the difference in response time.

The heterogeneous landscape of Florida Bay provides habitats for a variety of predators and prey. This dissertation examined the bottom-up transfer of affects from environmental variability through prey composition up to competition and predation affects on top predator distribution and foraging ecology in Florida Bay. Line transect surveys for bottlenose dolphins and seabirds were conducted in Florida Bay during the summer months of 2002 - 2005. Photo-identification techniques were implemented to identify individual dolphins. Synoptic with this survey effort, habitat characteristics (temperature, salinity, dissolved oxygen, turbidity, chlorophyll a, depth and bottom type) and prey composition (bottom trawl or gillnet) were sampled. Comparison of envelope maps from generalized additive models determined that predictive capacity of dolphin habitat did not improve by incorporating fish distribution data. However, models of dolphin distribution based solely on environmental proxies of fish distribution resulted in high predictive capacity. During the 2005 summer, shark distribution was sampled using a longline. The abundance of sharks was only correlated to fish catch from trawls on a regional scale. Larger sharks, of species that may threaten dolphins, were only caught in the Gulf zone of the Bay. Analysis of dolphin distribution revealed high individual site and foraging tactic fidelity. Dolphins were spatially coincident with habitat characteristics that encouraged the use of each individual's preferred foraging tactic. Depth was identified as the primary variable determining dolphin foraging tactic choice. Depth plays a significant role in the benthic composition of Florida Bay, which subsequently impacts prey communities and affects dolphin distribution, foraging and social ecology. Ordinations determined that fish distribution was also principally affected by depth and bottom type. Shallow environments frequently corresponded with mudbank habitat (depth < 1m) where the sighting rates of seabirds (cormorants, osprey, pelicans, terns) and foraging dolphins peaked. In conclusion, subtle relief in South Florida's bedrock topography dramatically affect benthic composition within Florida Bay, providing patchy habitats for prey and predators. The Florida Bay ecosystem will change with expected sea level rise, including spatial shifts of mudbank habitats. Top predator populations in Florida Bay will be forced to modify their distribution and foraging ecology accordingly. / Dissertation

Response times have been used to explore cognitive and perceptual processes since 1850 (Donders, 1868). The technique has primarily been applied to humans, birds, and terrestrial mammals. Results from two studies are presented here that examine response times in bottlenose dolphins (Tursiops truncatus) and white whales (Delphinapterus leucas). One study concerned response times to stimuli well above the threshold of perceptibility of a stimulus, and the other concerned response times to stimuli near threshold. Two white whales (Delphinapterus leucas) and five Atlantic bottlenose dolphins (Tursiops truncatus) were presented stimuli well above threshold. The stimuli varied in type (tone versus pulse), amplitude, duration, and frequency. The average response time for bottlenose dolphins was 231.9 ms. The average response time for white whales was 584.1 ms. There was considerable variation between subjects within a species, but the difference between species was also found to be significant. In general, response times decreased with increasing stimulus amplitude. The effect of duration and frequency on response time was unclear. Two white whales (Delphinapterus leucas) and four Atlantic bottlenose dolphins (Tursiops truncatus) were given audiometric tests to determine masked hearing thresholds in open waters of San Diego Bay (Ridgway et al., 1997). Animals were tested at six frequencies over a range from 400 Hz to 30 kHz using pure tones. Hearing thresholds varied from 87.5 dB to 125.5 dB depending on the frequency, masking noise intensity and individual animal. At threshold, median response time across frequencies within each animal varied by about 150 ms. The two white whales responded significantly slower (∼670 msec, p<0.0001) than the four dolphins (∼410 msec). As in terrestrial animals, reaction time became shorter as stimulus amplitude increased (Wells, 1913; Stebbins, 1966). Across the two studies, the dolphins as a group were faster in the abovethreshold study than in the nearthreshold study. White whales had longer response times than bottlenose dolphins in both studies. Analysis of response time with an allometric relation based on weight shows that the difference in weight can explain a significant part of the difference in response time.

This thesis examines the biology, ecology and anthropogenic threats of Indo-Pacific bottlenose dolphins (Tursiops aduncus) off Zanzibar, Tanzania, based on research conducted and samples collected between 2000 and 2008. Distribution and occurrence are described based on incidental catches (bycatch) in gillnet fisheries. Biology and ecology are examined by ageing and studying the reproductive biology and stomach contents of collected specimens. The composition of organohalogen compounds is determined in blubber samples, and assessment and mitigation of bycatch are conducted using observers onboard fishing vessels. Fisheries bycatch data showed that Indo-Pacific bottlenose dolphins occur year round in all areas around Zanzibar. Sexual maturity was attained between 7 and 8 years and body length 190-200 cm in females and at 16 years and body length 213 cm in males. The gestation period was estimated to be 12.3 months, with calving occurring throughout the year, peaking November-March and with an interval of 2.7 years. The estimated pregnancy rate was between 0.10 and 0.58 depending on methods used. Stomach contents revealed a relatively large number of prey species, but that only a few small- and medium-sized neritic fish and cephalopods contribute substantially to the diet. Estimates of total annual bycatch were &gt;9% which is not considered sustainable. An experiment showed that pingers can be a short term mitigation measure to reduce bycatch of dolphins in both drift- and bottom set gillnets. Methoxylated polybrominated diphenyl ethers (Meo-BDEs) were found at higher concentrations than anthropogenic organic pesticides (OCPs), with only traces of polychlorinated biphenyls (PCBs) detected. This study reveals the magnitude and apparent susceptibility of Indo-Pacific bottlenose dolphins off Zanzibar to anthropogenic threats, especially fisheries bycatch, and it is clear that immediate conservation and management measures are needed to reduce bycatch. / At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 2: Submitted. Paper 4: Manuscript. Paper 5: Submitted.

In recent years there has been an increased interest in measuring animal personality. It is argued that personality in animals is expressed through the behaviours they display. In this study personality has been investigated in a group of eight captive bottlenose dolphins (Tursiops truncatus). Data from focal samplings were analysed by using behavioural codings and the Five-factor model consisting of Openness to experience, Conscientiousness, Extraversion, Agreeableness and Neuroticism. The results revealed that the dolphins display both distinct personality differences as well as similarities in these factors. By calculating coefficients of association it was found that the dolphins also prefer the company of certain individuals over others. Knowledge of individual personality differences and its implications can be helpful in aspects such as management and reintroduction programs, evolution and genetics and in providing a complementary perspective to explain other behavioural and cognitive studies.

The objective of this thesis was to determine if there were any correlations in the distribution of cetaceans, especially sperm whales (Physeter macrocephalus), pantropical dolphins (Stenella attenuata), bottlenose dolphins (Tursiops truncatus), and Risso’s dolphins (Grampus griseus) in the northern Gulf of Mexico because of the influence of oil and gas production and exploration. This research is important because of the lack of knowledge about the impact of anthropogenic sounds from oil and gas exploration and production (E&P) on cetacean distribution in the Gulf of Mexico. I analyzed cetacean visual line-transect survey results from the Gulf of Mexico for period of 1992 – 2001. I divided this time span into an early period (1992 – 1997) and a late period (1998 – 2001). I overlayed the locations of cetacean sightings and the locations of oil and gas E&P platforms to demonstrate their close association in space, and tested a hypothesis that distribution would shift south correlated with changes in distribution of E&P. I compared the distributions of cetacean sightings in the entire Gulf of Mexico, and separated the Gulf of Mexico into east and west, between the early period and the late period. The east Gulf of Mexico represents an area without oil and gas E&P platforms and the west Gulf of Mexico is the area where oil and gas E&P platforms are located. The null hypothesis for these tests was that there was no difference in cetacean distribution between the early period and the late period. I also compared the distribution of sperm whales, pantropical dolphins, bottlenose dolphins, and Risso’s dolphins in the entire Gulf of Mexico, both east and west, between the early and late periods. I expect if distribution changes were correlated with changes in E&P distribution, then there will be a shift south in the western Gulf. Changes in distribution in the eastern Gulf would not be correlated with E&P. I found that the sightings per unit effort (SPUE) of all cetacean sightings in the entire Gulf of Mexico shifted to the south in the late period, as compared to the early period .The distribution of all cetacean sightings for the late period (1998-2001) was significantly different compared to the distributions of all cetacean sightings in the early period (1992-1997). The SPUE of sperm whale and bottlenose dolphin distributions were shifted to the north in the late period (1998-2001) compared to the early period (1992-1997). While pantropical dolphin distributions were significantly shifted to the south between the two time periods. I observed that the SPUE of all cetaceans sightings in the east Gulf of Mexico for the early period (1992-1997) were shifted to the south compared to the west, which were not different. The SPUE for sperm whales in the east for the early period were marginally shifted to the north in comparison to the west, which were also shifted further north. The SPUE for Risso’s dolphins in the east were shifted further north while in the west SPUE were shifted to the south. The SPUE for pantropical dolphin sightings were shifted to the north in both the east and west regions. While the SPUE for bottlenose dolphin sightings were shifted to the north in both the east and west Gulf of Mexico. My SPUE results suggest that pantropical dolphins, like the total cetaceans examined here, have shifted their distributions in the entire northern Gulf of Mexico to the south. However, in areas of high oil and gas E&P platforms the distributions of sperm whales and bottlenose dolphins did not shift their distributions from the early period to the late period to the south even though these E&P activities have shifted to the south over the past two decades as they expanded into the deeper waters of the northern Gulf of Mexico. Therefore, there is little evidence that large scale changes in the latitudinal distribution of marine mammals in the Northern Gulf have occurred as a result of greater offshore E&P activity.

Bottlenose dolphins inhabiting coastlines and estuaries in Florida have been impacted in the past decade by development, algal blooms, catastrophic pollution, and fishery interactions (FI). Dolphins react to disturbance and environmental stressors by modifying their movements and habitat use, which may put them in jeopardy of conflict with humans. FI plays an increasing role in contributing to dolphin mortalities. I investigated dolphin movements, habitat use, residency patterns, and frequency of FI with sport fishing. Tagging studies with short-term data tags and bolt on radio-transmitters were done in several locations in Florida and the east coast providing fine-scale measurements of swimming, daily travels, and foraging activity. Transit speeds agreed with the predicted mean cost of transport as dolphins spent much of their day and night travelling and resting while swimming. Increased foraging was detected by stomach temperature changes revealing dolphins fed at night with a peak starting just after sunset. Dolphin abundance, site fidelity, ranging, stranding mortality, and community structure was characterized at Choctawhatchee and Pensacola Bays in the Florida Panhandle via surveying and photo-identification. Results showed they made frequent inshore movements, maintained site fidelity to specific areas, and comprised several distinct communities. FI was assessed at offshore reefs and coastal fishing piers near Destin, FL and Orange Beach, AL, showing that some dolphins demonstrate affinity to this activity. Harmful interactions with dolphins on reefs and at fishing piers negatively affect their resident communities. Mitigation of FI is suggested by use of avoidance strategies, gear modifications, and improved fish release practices.

The St. Johns River (SJR; Jacksonville, FL, USA) is a large, brackish, estuarine system characterized by considerable anthropogenic pollution, recurrent harmful algal blooms (HABs), and diverse toxin-producing cyanobacteria. The most prevalent toxins in SJR water samples are microcystins/nodularins (MCs/NODs). Additionally, the SJR provides critical habitat for a genetically and behaviorally distinct estuarine community of bottlenose dolphins (Tursiops truncatus) that routinely uses and strands in low mesohaline and oligohaline areas of the river. This population has been subject to two unusual mortality events (UME) since 2010 and has since been described as having substantial declines in population health, characterized by widespread dermatitis and emaciation. Additionally, three dolphins have been recovered from low salinity habitats with epidermal algal mats. Because dolphin illness and strandings overlapped temporally and spatially with confirmed cyanobacterial blooms in the SJR, there is concern that estuarine dolphin health may be declining due to exposure to toxic cyanobacteria and HAB events. Specific to this study, the SJR estuarine community was considered a high-risk group for cyanotoxin exposure in relation to coastal animals. This study analyzed all available hepatic tissues for estuarine dolphins, and used samples from coastal individuals that stranded outside of the known cyanotoxin bloom season as controls. Three analytical methods were used to determine MCs/NODs presence in dolphin liver and epidermal algal mat samples. An Adda ELISA and LC-MS/MS were used to determine free MCs/NODs presence while the MMPB technique was used to determine total (bound and free) concentrations and as confirmatory analyses. ELISA analyses produced high values that were not supported by concurrent LC-MS/MS or MMPB analyses, indicative of false positives. MMPB testing resulted in low-level total MCs/NODs detection in some specimens. Results indicate that both estuarine and coastal dolphins are exposed to MCs/NODs, with potential toxic and immune health impacts.

Bottlenose dolphins (Tursiops truncatus) and other odontocete cetaceans rely on sound for communication, navigation, and foraging. Therefore, hearing is one of their primary sensory modalities. Both natural and anthropogenic noise in the marine environment could mask the ability of free-ranging dolphins to detect sounds, and chronic noise exposure could cause permanent hearing losses. In addition, several mass strandings of odontocete cetaceans, especially beaked whales, have been correlated with military exercises involving mid-frequency sonar, highlighting unknowns regarding hearing sensitivity in these animals.Auditory evoked potential (AEP) methods are attractive over traditional behavioral methods for measuring the hearing of marine mammals because they allow rapid assessments of hearing sensitivity and can be used on untrained animals. The goals of this study were to 1.) investigate the differences among underwater AEP, in-air AEP, and underwater behavioral heari ng measurements using two captive bottlenose dolphins, 2.) investigate the hearing abilities of a population of free-ranging bottlenose dolphins in Sarasota Bay, Florida, using AEP techniques, and 3.) report the hearing abilities of a stranded juvenile beaked whale (Mesoplodon europaeus) measured using AEP techniques.For the two captive dolphins, there was generally good agreement among the hearing thresholds determined by the three test methods at frequencies above 20 kHz. At 10 and 20 kHz, in-air AEP audiograms were substantially higher (about 15 dB) than underwater behavioral and underwater AEP audiograms.For the free-ranging dolphins of Sarasota Bay, Florida, there was considerable individual variation, up to 80 dB between individuals, in hearing abilities. There was no relationship between age, gender, or PCB load and hearing sensitivities. Hearing measured in a 52-year-old captive-born bottlenose dolphin showed similar hearing thresholds to the Sarasota dolphins up to 80 kHz, but exhibited a 50 dB drop in sensitivity at 120 kHz.Finally, the beaked whale was most sensitive to high frequency signals between 40 and 80 kHz, but produced smaller evoked potentials to 5 kHz, the lowest frequency tested. The beaked whale hearing range and sensitivity were similar to other odontocetes that have been measured.

Human interaction greatly influences the behavior and distribution of bottlenose dolphins (Tursiops truncatus). This project focuses on the distribution and behavior of bottlenose dolphins in the Drowned Cayes, Belize. Prior to the 2000s, the area was relatively undeveloped and undisturbed and had minimal human activity. Since the turn of the millennium, development and ecotourism activity has flourished in the area, increasing by more than 800,000 visitors from 1998-2006. Boat-based surveys were conducted in 2015 and were combined with previous survey data collected from 2005-2012 and compared to behavioral survey results from 1999-2000. Total dolphin observation time as a percent of total survey time and average number of dolphins per sighting were 17.2% and 2.7 in 1999-2000 and 10.8% and 1.6 for 2005-2015. The low number of dolphins and the low observation times suggest that the dolphin population in the Drowned Cayes have decreased since the 1990s. Eighty-nine percent of the total observation time for 2015 occurred on days in which there were zero cruise ships in the area suggesting that this decline may be in relation to increased human activity. Furthermore, foraging was the main behavior observed for both 1999-2000 and 2005-2015 data sets, suggesting that the Drowned Cayes area is used as a foraging ground. However, in 1999-2000 the foraging percentage was significantly higher than the 2005-2015 data set, dropping 28.9% and there was a 23.6% increase in traveling behavior between the two data sets. This could be a result of increased human activity. Additionally, survey photographs and results were used in the creation of the first dolphin photo identification database for the country. The guidelines used for photo analysis for photo quality and fin distinctiveness were tested to determine if they are easy to use and give consistent and reliable results regardless of judge. An intraclass correlation model calculated substantial agreement (ICC = 0.7) between judges’ scores, demonstrating consistent results, regardless of experience level. Therefore, the guideline can be used as a standard among multiple researchers.