Any software development process deals with four main artifacts namely; requirement, design, implementation and test. Depending upon the functionality of a particular product there might be variants present in these artifacts. These variants influence all the artifacts involved in a software development process. Data in the higher level artifact affects the data present in the further artifacts and is also refined when we move towards the lower level of abstraction. This thesis deals with the handling of all the variant information present in all the artifacts. Verification and consistency checks on this information were to be automated for making the development process easier. The results achieved during this thesis discuss the solutions for the problem of inconsistent variant information present in all the artifacts. By defining the extension of the intermediate format to support the variant information at Vector Informatik GmbH this problem has been resolved. The data used during the development is the variant information. The generic intermediate format has been extended in a way so that it can further support a variety of use cases. Along with the formulation of a format, documentation of variant information and methods to extract variant information form C source code are also discussed.

In some industrial fields, such as aerospace, electro-hydrostatic actuators (EHAs) are increasingly used to replace conventional standard hydraulic actuators due to their better energy performance. Moreover, implementing different type or technology of actuators in redundant actuation systems working on the same moving part introduced some new challenges. This paper presents a force-tracking controller for an asymmetric electro-hydrostatic actuator that is submitted to an external motion generated by an external source. In this case, the rod displacement is considered as an external disturbance for the hydraulic cylinder, but it is assumed that this disturbance can be easily measured using sensors. The theoretical motivation of this work is discussed along and a variable gain state feedback control based on Linear Parameter Varying control (LPV) theory is proposed to achieve stability, disturbance rejection and tracking performance. The Linear Matrix Inequalities (LMI) framework is used to determine a control law including an augmented state feedback with an integral action that reduces trajectory-tracking errors. Simulation results of the control law are finally given to verify the global performance of this control design.

Linhagens de Escherichia coli enterotoxigênica (ETEC) são consideradas como importante agente de diarréia, principalmente entre crianças e turistas em países em desenvolvimento. Entre os fatores de virulência expressos por ETEC, as enterotoxinas termo-lábil (LT) e termo-estável (ST) representam os mais relevantes fenótipos. Evidências preliminares sugerem que a severidade da diarréia associada a linhagens de ETEC deve refletir a diversidade natural de linhagens selvagens quanto à produção de enterotoxinas e/ou à ocorrência de variantes naturais com efeitos tóxicos reduzidos. No presente trabalho, investigamos diversidade genética do óperon etx, que codifica para a toxina LT, e da capacidade de produção e secreção de LT por linhagens de ETEC isoladas de humanos ou suínos em diferentes regiões geográficas. Os resultados mostraram considerável variabilidade na produção de LT com valores variando de 2 a 2.525 ng de toxina por mL de cultura. Secreção de LT foi também variável com valores variando de menos que 0,04% a 49,5% do total de LT produzida pelas diferentes linhagens de ETEC. Adicionalmente, experimentos de alça ligada em coelho mostraram uma boa correlação entre a quantidade de LT secretada sob condições in vitro e a capacidade de causar acúmulo de fluidos in vivo. Nós determinamos ainda diversidade de ETEC pela obtenção das seqüências dos óperons etxAB de 50 linhagens (LT+ or LT+/ST+) pertencentes a diferentes sorotipos com ênfase para as linhagens produtoras apenas de LT e isoladas de crianças assintomáticas. As seqüências de nucleotídeo completas dos genes etxAB revelaram 23 alterações de aminoácidos nas subunidades A (18) e B (5), as quais geraram 16 variantes de LT. Entre estes variantes de LT, um mostrou efeito tóxico reduzido em comparação à toxina de referência LT1. A forma de LT atenuada (LT4) tem atividade enzimática reduzida devido à troca de aminoácido. / Enterotoxigenic Escherichia coli (ETEC) strains represent an important etiological agent of diarrheal disease, particularly among children and travelers in developing countries. Among the virulence factors expressed by ETEC strains the heat-labile (LT) and heat-stable (ST) enterotoxins represent the most revelevant phenotypes. Indirect evidences suggest that the severity of diarrhea associated to ETEC strains might reflect the natural diversity of wild strains to produce enterotoxins and/or the occurrence of variants endowed with reduced toxic effects. In the present study, we investigated both the genetic diversity of the etx operon, encoding the heat-labile toxin, and the capability to produce/secrete LT by ETEC strains isolated from humans or porcine in different geoghrafic areas. The results showed a remarkable variability on the production of LT with values ranging from 2 to 2,525 ng of toxin per ml of culture. LT secretion was also variable with values ranging from less than 0.04% to 49.5% of total LT produced by the different ETEC strains. Additionally, rabbit ileal loop experiments showed a good correlation between the amounts of secreted LT under in vitro conditions and fluid accumulation in vivo. We determined also the diversity of the etxAB operon of 50 ETEC strains (LT+ or LT+/ST+) belonging to different serotypes with emphasis to LT+-only producing strains isolated from asymptomatic children. The complete nucleotide sequences of the etxAB genes revealed 23 amino acid changes at the A (18) or B (5) subunits, which generated 16 variant forms of LT. Among these LT variants, one of them showed reduced toxic effects in comparison to the reference toxin LT1. The attenuated LT form (LT4) had decreased enzymatic activity due to an amino acid replacement (K4R) at the A1 subunit. LT4 retains its immunogenic and adjuvant properties following nasal immunization. Additionaly, the LT4 variant showed altered immune modulatory features and promoted a more biased Th1 response, which favor activation of effector CD8+ T lymphocytes, to co-administred antigen with regard to LT1. Taken together, our results demonstrate that ETEC strains isolated from human subjects express natural genetic variability leading to a remarkable polymorphism of the etx operon as well as production and secretion of LT. Such natural genetic diversity observed in ETEC strains may affect the host-pathogen relationships and, consequently, contribute to the severity of the disease among infected subjects.

Made available in DSpace on 2016-04-28T19:34:27Z (GMT). No. of bitstreams: 1 Rosangela Xavier dos Anjos.pdf: 543500 bytes, checksum: 619d773b23c1bc30fd9a27178278d620 (MD5) Previous issue date: 2008-05-16 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The purpose of this work is to present, under a Sociolinguistic point of view, contemporary theories regarding linguistic variation, having the Brazil northeastern variant as spoken by the students of Educação para Jovens e Adultos school (E.J.A.). The research has as sample to investigation, interviews given by Brazil northeastern students and its proposal is to observe the northeast vocabulary as a way of expression of the northeast culture and customs. The main objective is to verify the treatment given to the northeastern variant on classroom, as seen from the student s perspective. To meet the ends of this research, the interview method was chosen, aiming at getting elucidative data to the present work. The field task is performed by means of recording individual interviews with the E.J.A´s northeastern students. It was verified, through the research, that there is a linguistic richness and peculiarity on E.J.A, most of all, as regards to people immigration from Brazil northeastern different states. The northeast variant is part of a linguistic reality, being in that way, an object of study, because it is present at the most different scholarship contexts / Nesta dissertação propomo-nos apresentar, numa visão Sociolingüística, as teorias contemporâneas relativas à Variação lingüística, tendo como abordagem a variante nordestina falada pelos alunos da Educação de Jovens e Adultos (E.J. A.). A pesquisa tem como amostra para investigação as entrevistas concedidas pelos alunos nordestinos e como proposta observar o vocabulário nordestino como meio de expressão da cultura e dos costumes da Região Nordeste. O objetivo principal é o de verificar o tratamento dado à variante nordestina em sala de aula, observadas pela perspectiva do aluno. Para a realização da pesquisa, adotamos a metodologia entrevista, com o intuito de coletar dados elucidativos para o presente estudo. O trabalho de campo é efetuado por meio de gravação de entrevistas individuais com alunos nordestinos da E.J.A. Verificamos, por meio da pesquisa, que na E.J.A. há uma peculiaridade e riqueza lingüística, sobretudo, no que diz respeito à imigração de pessoas de diferentes estados do Nordeste. A variante nordestina faz parte de uma realidade lingüística, sendo, pois, passível de estudo, por se fazer presente nos mais diferentes contextos escolares

Introdução: No Brasil há carência de instrumentos validados para a análise do curso da Demência Frontotemporal (DFT). Dessa forma, torna-se relevante a validação da Escala de Estadiamento e Progressão da Demência Frontotemporal (FTD-FRS). Em nosso meio, as escalas de estadiamento das demências, como a Clinical Dementia Rating (CDR), foram elaboradas para graduar a doença de Alzheimer (DA) e não incluem os sintomas específicos da DFT. Objetivos: 1. Realizar a tradução, adaptação transcultural e validação da FTD-FRS para o contexto brasileiro. 2. Avaliar a capacidade da FTD-FRS detectar alterações em pacientes com DFTvc, afasia progressiva primária (APP) e DA após 12 meses da avaliação inicial, em comparação com a escala CDR para DLFT, e com a CDR original. Métodos: Participaram do estudo 101 indivíduos com idade igual ou superior a 40 anos, com escolaridade formal acima de dois anos, sendo 31 pacientes com diagnóstico de DFT variante comportamental (DFTvc), doze pacientes com afasia progressiva primária (APP), 28 pacientes com doença de Alzheimer (DA), oito com comprometimento cognitivo leve (CCL) e 22 controles normais (CN). Foram entrevistados os familiares ou cuidadores que tinham contato frequente com o paciente. Os pacientes com DA, e com os subtipos de DFT foram pareados quanto à gravidade da doença, segundo a CDR. Resultados: Foi realizado o processo de adaptação transcultural da FTD-FRS. Consistiu em: tradução, retrotradução (realizadas por tradutores independentes), discussão com especialistas sobre a versão em português e equivalência com a versão original, e desenvolvimento da versão final. A consistência interna da FTD-FRS, estimada pelo alfa de Cronbach foi 0,975, e o coeficiente de correlação intra-classe, para a estabilidade no teste e reteste em seis meses foi de 0,977. A análise fatorial revelou a existência de quatro fatores que se correlacionaram significativamente com os domínios da CDR-DLFT. Os pacientes com DFTvc apresentaram progressão mais rápida em 12 meses do que os demais subtipos de demência na FTD-FRS, na CDR-DLFT e na CDR-original. Considerações finais: A FTD-FRS tem propriedades psicométricas adequadas para seu uso clínico no Brasil. Este instrumento pode auxiliar na caracterização de sintomas clínicos relevantes para o diagnóstico e estadiamento da DFT. Também pode documentar os resultados relacionados à intervenção terapêutica. Este estudo fornece aos clínicos e pesquisadores um instrumento válido para estadiamento e acompanhamentode de pacientes diagnosticados com DFT / Introduction: In Brazil there is a shortage of validated instruments for the analysis of the course of Frontotemporal Dementia (FTD). Thus, the validation of the Frontotemporal Dementia Staging and Progression Scale (FTD-FRS) becomes relevant. In our setting, dementia staging scales, such as the Clinical Dementia Rating (CDR), were designed to stage Alzheimer\'s disease (AD) and did not include the specific symptoms of FTD. Objectives: 1. To perform the translation, cross-cultural adaptation and validation of the FTD-FRS for the Brazilian context. 2. Evaluate the ability of the FTD-FRS to detect changes in patients with bvFTD, primary progressive aphasia (PPA) and AD after 12 months of the initial evaluation, compared to the CDR scale for FTLD, and with the original CDR. Methods: A total of 101 individuals aged 40 years and older, with formal schooling above two years of age, were included in the study. Twenty-one patients were diagnosed with bvFTD, twelve patients with PPA, 28 AD, eight with mild cognitive impairment (MCI) and 22 normal controls (NC). Family members or caregivers who had frequent contact with the patient were interviewed. Patients with AD and with FTD subtypes were matched for disease severity, according to CDR. Results: The process of cross-cultural adaptation of the FTD-FRS was carried out. It consisted of: translation, back-translation (carried out by independent translators), discussion with experts about the Portuguese version and equivalence with the original version, and development of the final version. The internal consistency of the FTD-FRS, estimated by the Cronbach\'s alpha was 0.975, and the intra-class correlation coefficient for the test and retest stability at six months was 0.977. Factor analysis revealed the existence of four factors that correlated significantly with the CDR-DLFT domains. Patients with bvFTD showed faster progression at 12 months than the other dementia subtypes in the FTD-FRS, CDR-DLFT and CDR-original version scales. Final considerations: FTD-FRS has psychometric properties suitable for clinical use in Brazil. This instrument may aid in the characterization of clinical symptoms relevant to the diagnosis and staging of FTD. It can also document the results related to therapeutic interventions. This study provides clinicians and researchers with a valid instrument for staging and follow-up of patients diagnosed with FTD

Linhagens de Escherichia coli enterotoxigênica (ETEC) são consideradas como importante agente de diarréia, principalmente entre crianças e turistas em países em desenvolvimento. Entre os fatores de virulência expressos por ETEC, as enterotoxinas termo-lábil (LT) e termo-estável (ST) representam os mais relevantes fenótipos. Evidências preliminares sugerem que a severidade da diarréia associada a linhagens de ETEC deve refletir a diversidade natural de linhagens selvagens quanto à produção de enterotoxinas e/ou à ocorrência de variantes naturais com efeitos tóxicos reduzidos. No presente trabalho, investigamos diversidade genética do óperon etx, que codifica para a toxina LT, e da capacidade de produção e secreção de LT por linhagens de ETEC isoladas de humanos ou suínos em diferentes regiões geográficas. Os resultados mostraram considerável variabilidade na produção de LT com valores variando de 2 a 2.525 ng de toxina por mL de cultura. Secreção de LT foi também variável com valores variando de menos que 0,04% a 49,5% do total de LT produzida pelas diferentes linhagens de ETEC. Adicionalmente, experimentos de alça ligada em coelho mostraram uma boa correlação entre a quantidade de LT secretada sob condições in vitro e a capacidade de causar acúmulo de fluidos in vivo. Nós determinamos ainda diversidade de ETEC pela obtenção das seqüências dos óperons etxAB de 50 linhagens (LT+ or LT+/ST+) pertencentes a diferentes sorotipos com ênfase para as linhagens produtoras apenas de LT e isoladas de crianças assintomáticas. As seqüências de nucleotídeo completas dos genes etxAB revelaram 23 alterações de aminoácidos nas subunidades A (18) e B (5), as quais geraram 16 variantes de LT. Entre estes variantes de LT, um mostrou efeito tóxico reduzido em comparação à toxina de referência LT1. A forma de LT atenuada (LT4) tem atividade enzimática reduzida devido à troca de aminoácido. / Enterotoxigenic Escherichia coli (ETEC) strains represent an important etiological agent of diarrheal disease, particularly among children and travelers in developing countries. Among the virulence factors expressed by ETEC strains the heat-labile (LT) and heat-stable (ST) enterotoxins represent the most revelevant phenotypes. Indirect evidences suggest that the severity of diarrhea associated to ETEC strains might reflect the natural diversity of wild strains to produce enterotoxins and/or the occurrence of variants endowed with reduced toxic effects. In the present study, we investigated both the genetic diversity of the etx operon, encoding the heat-labile toxin, and the capability to produce/secrete LT by ETEC strains isolated from humans or porcine in different geoghrafic areas. The results showed a remarkable variability on the production of LT with values ranging from 2 to 2,525 ng of toxin per ml of culture. LT secretion was also variable with values ranging from less than 0.04% to 49.5% of total LT produced by the different ETEC strains. Additionally, rabbit ileal loop experiments showed a good correlation between the amounts of secreted LT under in vitro conditions and fluid accumulation in vivo. We determined also the diversity of the etxAB operon of 50 ETEC strains (LT+ or LT+/ST+) belonging to different serotypes with emphasis to LT+-only producing strains isolated from asymptomatic children. The complete nucleotide sequences of the etxAB genes revealed 23 amino acid changes at the A (18) or B (5) subunits, which generated 16 variant forms of LT. Among these LT variants, one of them showed reduced toxic effects in comparison to the reference toxin LT1. The attenuated LT form (LT4) had decreased enzymatic activity due to an amino acid replacement (K4R) at the A1 subunit. LT4 retains its immunogenic and adjuvant properties following nasal immunization. Additionaly, the LT4 variant showed altered immune modulatory features and promoted a more biased Th1 response, which favor activation of effector CD8+ T lymphocytes, to co-administred antigen with regard to LT1. Taken together, our results demonstrate that ETEC strains isolated from human subjects express natural genetic variability leading to a remarkable polymorphism of the etx operon as well as production and secretion of LT. Such natural genetic diversity observed in ETEC strains may affect the host-pathogen relationships and, consequently, contribute to the severity of the disease among infected subjects.

Le texte spécialisé dans le domaine de la surdité est un phénomène complexe où les termes ont une fonction sémantique très importante. Le discours actualise le sens des termes et donne suite à de nouvelles significations dynamiques. Le corpus bilingue (français, arabe) est représentatif de différents types de discours et de niveaux de spécialisation notamment lorsqu’il s’agit de comparer la terminologie de la surdité entre les pays arabes (Liban, Syrie et Jordanie). Les termes, qui sont responsables de transmettre les connaissances relatives à une spécialité, constituent aujourd'hui un objet d'étude central pour la terminologie. Leur extraction s’effectue non seulement par la méthode manuelle mais aussi à travers les nouveaux logiciels d’extraction automatique. Cette thèse prend en considération les besoins linguistiques des usagers qui sont dorénavant les vrais consommateurs de terminologie. Elle a pour objectif de faire une approche socioterminologique et textuelle du domaine de la surdité en mettant la lumière sur les phénomènes étudiés comme la synonymie, la variation terminologique, la vulgarisation, la métaphore, la traduction et autres. Sa retombée étant la constitution d’une base de données terminologique trilingue qui répond aux exigences des spécialistes et des non-spécialistes. / The specialized text in the domain of deafness is a complex phenomenon where terms have important semantic functions. The discourse updates the meaning of terms and brings up new dynamic significations. The bilingual corpus (French, Arabic) is representative of different types of discourse and levels of specialization especially when it comes to comparing the terminology of deafness in the three Arab countries (Lebanon, Syria, Jordan). Terms in charge of transmitting knowledge of special fields represent nowadays a central object of study for terminology. The extraction of terms can be made manually but also by means of new automatic term extraction software. Our doctoral thesis takes into consideration the linguistic needs of language users that are considered from now on the real consumers of terminology. This thesis is intended for socioterminological and textual approaches of the domain of deafness. It highlights the studied phenomena such as synonymy, terminology variation, scientific popularization, metaphor, translation and many other phenomena. The result of the thesis research being the construction of a trilingual terminological data base, it meets the requirements of specialists and non-specialists.

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La mise en place d'une ligne de produits logiciels permet de construire et de maintenir une famille de produits logiciels similaires en mettant en œuvre des principes de réutilisation. Ces principes favorisent la réduction de l'effort de développement et de maintenance, raccourcissent le temps de mise sur le marché et améliorent la qualité globale du logiciel. La migration de produits logiciels similaires vers une ligne de produits demande de comprendre leurs similitudes et leurs différences qui s'expriment sous forme de caractéristiques (features) offertes. Dans cette thèse, nous nous intéressons au problème de la construction d'une ligne de produits à partir du code source de ses produits et de certains artefacts complémentaires comme les diagrammes de cas d'utilisation, quand ils existent. Nous proposons des contributions sur l'une des étapes principales dans cette construction, qui consiste à extraire et à organiser un modèle de caractéristiques (feature model) dans un mode automatisé. La première contribution de cette thèse consiste à extraire des caractéristiques dans le code source de variantes de logiciels écrits dans le paradigme objet. Trois techniques sont mises en œuvre pour parvenir à cet objectif : l'Analyse Formelle de Concepts, l'Indexation Sémantique Latente et l'analyse des dépendances structurelles dans le code. Elles exploitent les parties communes et variables au niveau du code source. La seconde contribution s'attache à documenter une caractéristique extraite par un nom et une description. Elle exploite le code source mais également les diagrammes de cas d'utilisation, qui contiennent, en plus de l'organisation logique des fonctionnalités externes, des descriptions textuelles de ces mêmes fonctionnalités. En plus des techniques précédentes, elle s'appuie sur l'Analyse Relationnelle de Concepts afin de former des groupes d'entités d'après leurs relations. Dans la troisième contribution, nous proposons une approche visant à organiser les caractéristiques, une fois documentées, en un modèle de caractéristiques. Ce modèle de caractéristiques est un arbre étiqueté par des opérations et muni d'expressions logiques qui met en valeur les caractéristiques obligatoires, les caractéristiques optionnelles, des groupes de caractéristiques (groupes ET, OU, OU exclusif), et des contraintes complémentaires textuelles sous forme d'implications ou d'exclusions mutuelles. Ce modèle est obtenu en exploitant une structure obtenue par Analyse Formelle de Concepts appliquée à la description des variantes par les caractéristiques. L'approche est validée sur trois cas d'étude principaux : ArgoUML-SPL, Health complaint-SPL et Mobile media. Ces cas d'études sont déjà des lignes de produits constituées. Nous considérons plusieurs produits issus de ces lignes comme s'ils étaient des variantes de logiciels, nous appliquons notre approche, puis nous évaluons son efficacité par comparaison entre des modèles de caractéristiques extraits automatiquement par l'approche présentée et les modèles de caractéristiques initiaux (conçus par les développeurs des lignes de produits analysées).