The problem of this study is to determine the vitamin B-6 status of patients who have chronic obstructive pulmonary disease (COPD). Erythrocyte aspartate transaminase assay was the method for measuring vitamin B-6 status. The vitamin B-6 status was examined in thirty subjects (ten COPD subjects and twenty control subjects). An unpaired t-test was used to compare the vitamin B-6 status of the COPD group versus the control group. Four determinants (percentage stimulation, ratio of basal to stimulated activity, basal activity, and stimulated activity) were used to determine vitamin B-6 status in both groups of subjects. Percentage stimulation and ratio of basal to stimulated activity were not significantly different (control group versus COPD group) at the .05 level. However, two of ten COPD subjects had values for percentage stimulation that were two standard deviations above the mean, indicating a poor B-6 status. In contrast, basal activity and stimulated activity of erythrocyte aspartate transaminase were found to be significantly lower at the .05 level in the COPD group than the control group. Therefore, the COPD subjects as a group had some biochemical characteristics of a lower level of vitamin B-6 than the controls.

Methionine dependence, the inability of cells to grow when the amino acid methionine is replaced in culture medium by its metabolic precursor homocysteine, is characteristic of many cancer cell lines. Most cells proliferate normally under these conditions. The methionine dependent tumorigenic human melanoma cell line MeWo-LC1 was derived from the methionine independent non-tumorigenic line MeWo. The MeWo-LC1 cell line has been shown to have a cellular phenotype similar to that of cells from patients with the cblC inborn error of cobalamin metabolism, with decreased synthesis of cobalamin coenzymes and decreased activity of the cobalamin dependent enzymes methionine synthase and methylmalonyl-CoA mutase. Inability of cblC cells to complement the defect in cobalamin metabolism in MeWo-LC1 suggested that the defect was caused by decreased activity of the MMACHC gene product. However, no potentially disease causing mutations could be detected in the coding sequence of MMACHC in MeWo-LC1. No MMACHC expression could be detected in MeWo-LC1, and there was virtually complete methylation of a CpG island at the 5' end of the MMACHC gene in MeWo-LC1, consistent with inactivation of the gene by methylation; the CpG island was partially methylated in MeWo and only lightly methylated in control fibroblasts. Transfection of MeWo-LC1 with wild type MMACHC with a constitutive promoter resulted in correction of the defect in cobalamin metabolism and restoration of the ability of cells to grow in medium containing homocysteine. We conclude that epigenetic inactivation of the MMACHC gene is responsible for methionine dependence in MeWo-LC1.

Diss. (sammanfattning) Stockholm : Karolinska institutet, 2007. / Härtill 4 uppsatser.

Diss. (sammanfattning) Stockholm : Karolinska institutet, 2006. / Härtill 5 uppsatser.

Catosal ist ein Produkt, das weit verbreitet zur Behandlung von sekundären Keto-sen sowie zu Gebärpareseprophylaxe und Behandlung von Fruchtbarkeitsstörungen eingesetzt wird. Zielstellung: Ziel dieser Studie war es, in zwei Versuchsanordnungen (Studie 1 und Studie 2) zu prüfen, ob Catosal Einfluss auf den klinischen Allgemeinzustand und den Stoffwechsel von Milchkühen in Phasen hoher Belastung nimmt. Versuchsanordnung: Studie 1: Es wurden 30 Kühe mit linksseitiger Dislocatio a-bomasi aus dem Patientengut der Medizinischen Tierklinik Leipzig untersucht. Diese wurden alternierend den beiden Gruppen VG 1 und KG 1 zugeteilt. Der VG 1 wurde vor der Operation 25 ml Catosal intravenös verabreicht. Die KG 1 diente zur Kon-trolle und blieb unbehandelt. In den darauffolgenden Untersuchungen wurden der klinische Allgemeinzustand sowie klinisch-chemische und weitere Parameter unter-sucht. Studie 2: In die Studie wurden 79 Kühe im peripartalen Zeitraum aus einem Milchviehbetrieb in der Nähe von Leipzig einbezogen. Diese wurden alternierend drei Gruppen zugeordnet. VG 2 (n=26) wurde 10 d ante partum (ap) und 3 d post partum (pp) je 25 ml Catosal subcutan (s.c.) verabreicht. Der VG 3 (n=28) wurden 3 d pp und 5-7 d pp je 25 ml Catosal s.c. verabreicht. Die KG 2 (n=25) diente zur Kontrolle und blieb unbehandelt. Jede der drei Gruppen hatte einen Anteil von je 10 Färsen. Das Untersuchungsspektrum wurde anhand der Ergebnisse des ersten Ansatzes modifiziert. Ergebnisse Studie 1: Zwischen VG 1 und KG 1 wurden folgende Unterschiede fest-gestellt: 1) Der klinische Allgemeinzustand verbesserte sich in der VG 1. Dies ließ sich anhand der signifikanten Unterschiede (p<0,05) bei Futteraufnahme und Wie-derkauverhalten (WKV) feststellen. Die Entwicklung der Puls (PF)- und Atemfre-quenzen (AF) in der VG 1 zeigte ein tendenziell (0,1>p>0,05) früheres Erreichen des physiologischen Bereichs. 2) Der Energie-Leber-Stoffwechsel zeigte für die VG 1 ei-ne günstigere Entwicklung. Dies wurde anhand des postoperativen Verlaufs der kli-nisch-chemischen Parameter ß-Hydroxybutyrat (BHB), Glucose, freie Fettsäuren (FFS), Bilirubin, Aspartat-Aminotransferase (ASAT), Glutamat-Dehydrogenase (GLDH), Creatinkinase (CK) und Harnstoff deutlich, wobei für BHB, FFS, ASAT so-wie CK signifikant (p<0,05) geringere Konzentrationen/Aktivitäten in der VG 1 bestanden. Die Gruppenunterschiede von Glucose, Bilirubin und GLDH waren nicht signifikant. 3) Die Vormagenmotorik verbesserte sich post op (0,1>p>0,05). Dies spiegelte sich in der für die VG 1 zeitweise (8 h; 10 h pp: p<0,05) signifikant höheren Pansenmotorik wider. 4) Der Rückgang der Bluteisenkonzentration fiel in der VG 1 post op nicht so stark wie in der KG 1 aus und lag 24 h post op signifikant (p<0,05) über jener der KG 1. Ergebnisse Studie 2: Folgende Unterschiede konnten zwischen den Versuchsgrup-pen VG 2 und VG 3 sowie der KG 2 festgestellt werden: 5) Die Parameter des Stoff-wechsels und des klinischen Allgemeinzustands befanden sich während des gesam-ten Untersuchungszeitraums in allen drei Gruppen im physiologischen Bereich. 6) Die Parameter des Energie-Fettstoffwechsels lagen im physiologischen Bereich und zeigten keine klinisch relevanten Unterschiede zwischen den Gruppen. 7) Körperin-nentemperatur (T), PF sowie AF lagen in der Gruppe VG 23 d pp signifikant (p<0,05) unter denen der VG 3 und KG 2, in allen drei Gruppen jedoch innerhalb des physio-logischen Bereiches. 8) Bei den Mineralstoffen Eisen und Calcium (Ca) konnten in der Gruppe VG 2 3 d pp signifikant (p<0,05) höhere Konzentrationen im Blut be-stimmt werden, wobei bei Ca die geringere Milchleistung der VG 2 berücksichtigt werden musste. 9) Die geringgradig höhere Leukozytenzahl sowie die zeitweise sig-nifikant (3 d pp: VG 2:VG 3: p<0,05) geringere Konzentration an Albumin bei gleicher Gesamteiweißkonzentration im Blut ergaben einen Hinweis auf eine Verbesserung der unspezifischen Immunabwehr. 10) Die Milchleistung der VG 3 lag 45 d pp signifi-kant (p<0,05) über der von VG 2 und KG 2. Auch 100 d pp bestand dieser Unter-schied noch. 11) Die Erstbesamung fand in der VG 2 und VG 3 früher als bei der KG 2 statt (0,1>p>0,05). Bei den Kühen war der Unterschied jedoch signifikant (VG 3:KG 2: p<0,05). Schlussfolgerung: Eine Beeinflussbarkeit einzelner Aspekte des Stoffwechsels sowie des klinischen Allgemeinzustands durch Catosal konnte mit der Studie 1 belegt werden. Bezüglich der Einflüsse von Catosal auf die Leistungsfähigkeit, den Stoffwechsel und den klinischen Allgemeinzustand im peripartalen Zeitraum sind die Ergebnisse in ihrer Gesamtheit nicht eindeutig und bedürfen weiterer Untersuchungen.

No description available.

Thesis (MTech (Food Technology))--Cape Peninsula University of Technology, 2016. / In South Africa malnutrition exists due to inadequate dietary intake of micronutrients which is one of the major causes of vitamin deficiencies leading to disease. The treatment of malnutrition over the past years has been a considerable burden on the South African economy. Therefore, food fortification is one of the current strategies used to minimize malnutrition by increasing the nutritional value of staple foods. Commercial dairy products and pharmaceutical nutritional products (food supplements) in South Africa have been developed and produced for affluent consumers. Hence the need to develop an affordable fortified dairy product for the majority of South Africans prompted this study aimed at using a “naturally” fortified kefir beverage with vitamin B12 and folate to increase B-vitamins levels. Since Propionibacterium freudenreichii and Streptococcus thermophilus are known to be good producers of vitamin B12 and folate, respectively, and propionibacteria has the ability to grow symbiotically in the presence of lactic acid bacteria, the inclusion of these organisms with the kefir grains was an achievable objective. In order to conduct the analysis of vitamin B12 and folate in the samples, sample extraction and HPLC assay techniques were developed. The extraction of vitamin B12 and folate were achieved by using KCN extraction buffer and the trienzymatic method, respectively. The samples were also subjected to purification and concentration using solid phase extraction for optimum results. All standards and samples were flushed with nitrogen gas and stored for a maximum of 2 weeks at –20°C to prevent B-vitamin deterioration. The HPLC assembly for the vitamin B12 analysis included a Luna C18 column and a diode array detector (DAD) for the detection and quantification. For the folate analysis it included a Zorbax SB-C18 and Luna C18 columns in tandem and the fluorescence detector (FLD) was used for the detection and quantification of THF, 5-CH3-THF and 5-CHO-THF, while the DAD was used for PGA and pteroyltri-γ-L-glutamic acid concentration in the samples.

Realizou-se um estudo descritivo conduzido no Hospital de Clínicas de Porto Alegre, no período de outubro de 2001 a outubro de 2002, com pacientes HIV positivos e anêmicos. Objetivo: avaliar a associação entre anemia e o perfil nutricional em uma amostra de pacientes HIV+ . Métodos: Foram incluídos 34 pacientes maiores de 18 anos, sendo todos pacientes diagnosticados com anemia. Foram analisados exames laboratoriais, avaliação da ingestão alimentar (recordatório alimentar de 24h), freqüência alimentar e coleta dos parâmetros antropométricos dos pacientes. Resultados: O recordatório alimentar de 24 horas demonstrou a deficiência na ingestão de folato pela maioria dos pacientes; enquanto que vitamina B12 e ferro estiveram de acordo com as RDA’s. Conclusão: A causa da anemia nestes pacientes talvez não tenha sido em função desta deficiente ingestão de folato, ainda mais por se tratar de um estudo descritivo. Ressalta-se a importância do profissional para o acompanhamento nutricional destes pacientes, para a promoção de um adequado estado nutricional e qualidade de vida. / Was develop a descritive study conducted in the Porto Alegre Clinics’ Hospital, from october 2001 to October 2002, with positive HIV and anemics patients. Objectives: Evaluate the association between anemia and nutritional status in positive HIV patients. Methodology: Were included 34 patients under 18 years old, all diagnosed with anemia. Laboratorial exams, evaluation of food intake (24h register), questionary of food intake frequence and anthropometrics data were collected. Results: The 24h register food intake showed the deficiency in folate intake by most of patients ,while vitamine B12 and iron intake were accorded RDA’s. Conclusions: The etiology of anemia in these patients maybe wasn´t cause by this folate deficiency, also because this was a describe study. The presence of a professional appears to be important for the nutritional treatment of these patients, to develop a health nutrional status and quality of life.

Vitamin B₁₂ deficiency has been difficult to diagnose, mainly due to the vitamin's lack of biological significance in serum in which it is usually assayed. This research has investigated the marker of vitamin B₁₂/cobalt (Co) deficiency in sheep, methylmalonic acid (MMA), in comparison with serum and liver vitamin B₁₂ concentrations in farm situations where vitamin B₁₂ deficiency is expected in order to establish more accurate reference ranges for serum and liver vitamin B₁₂, and MMA. In addition, an attempt was made to ascertain the vitamin B₁₂ requirements of preruminant (PR) lambs, and to determine whether metabolic demand for vitamin B₁₂ influences tissue concentrations. Furthermore, since the vitamin is active in biological tissues in form of its coenzymes, 5’ -deoxyadenosylcobalamin and methylcobalamin, a preliminary assessment of variation in the distribution of these coenzymes in liver in different situations has been sought. The first trial was set up to find out if the addition of propionate to the PR lamb's diet stimulated the uptake and/or storage of vitamin B₁₂ in the liver as a reflection of the need to deal with the incoming propionate. Sixteen ten day old lambs (Dorset Down/Coopworth cross-bred) were housed indoors soon after birth and fed on milk replacer. For half of the lambs 7.5 % (w:w) of the milk powder was replaced by propionate. Within each group, four lambs were treated with 250 µg vitamin B₁₂ twice weekly. Supplementation with vitamin B₁₂ increased liver concentrations from ~250 to ~900 nmol/kg fresh tissue, but there was no effect of propionate. Propionate addition did, however, result in increased plasma vitamin B₁₂ concentrations in vitamin B₁₂ supplemented groups, values being 3323 and 2355 pmol/l in propionate supplemented and control groups, respectively. This suggested that diet could influence plasma vitamin B₁₂ concentrations. An attempt was made to quantify the PR lamb's ability to absorb vitamin B₁₂ from the alimentary tract by comparing the ability of intra-muscular (IM) and oral vitamin B₁₂ to raise plasma and liver vitamin B₁₂ concentrations. Twenty-seven three to four day old lambs from a farm with marginal Co status were housed indoors and fed on milk replacer. They were divided into three groups: control (n=3), IM treatment (n=12) and oral treatment (n=12). The two treatment groups were further subdivided into five sub-groups. These received, respectively, 0.2 (n=3), 0.4 (n=2), 0.8 (n=2), 1.6 (n=2) and 3.2 µg OH-cbl/d (n=3). The oral groups received tenfold the amount of the comparable IM groups, on the assumption that if oral absorption of the vitamin is about 10 % both groups would show similar increases in plasma and liver vitamin B₁₂ concentration. None of the IM groups showed any significant change in plasma or liver vitamin B₁₂. In the oral groups only the group on the highest dose of vitamin B₁₂, viz 32 µg/d, showed increases in plasma and liver concentrations. It was concluded that either absorption of vitamin B₁₂ was greater than 10 % or that the vitamin was retained better when administered orally. The amount retained in the livers of the lambs in the highest oral group was calculated to represent ~ 7.5 % of the dose. In a follow-up 24 h trial, 14 of the above lambs were divided into three groups: Control (n=3), oral (n=6) and IM (n=5) treatment. The IM group received 3.2 µg OH-cbl and the oral group tenfold the amount as single doses at 0800 h. Blood samples were taken at regular intervals throughout the 24 h period and assayed for vitamin B₁₂, Vitamin B₁₂ concentrations in the IM group rose steeply within the first hour after injection to a concentration that was calculated to reflect 100 % uptake of the vitamin. It rose more slowly over about 8 h in the oral group. From the area under the curve absorption of the oral dose was estimated to be ~ 7 %. The next experiment involved a farm where Co deficiency had been reported previously. In the first year, 50 pregnant two-tooth Half-bred ewes were divided randomly into two groups of 25. One group received a Co bullet plus 1000 µg OH-cb1 IM, the other group remained unsupplemented. In the following year the trial was repeated. Ewes from the previous year's trial (by then four-tooths) were augmented by a new cohort of pregnant two-tooths to make up numbers to 75. After lambing the lambs were divided into four groups: first by their dams' vitamin B₁₂ treatment, then half of each group received injections of vitamin B₁₂ at approximately three weekly intervals while the other half remained untreated. The trials lasted about five months, from mid-pregnancy until weaning. Pasture Co was at its lowest at lambing in both years, 0.09 and 0.10µg/g DM, respectively. In the first year, vitamin B₁₂ concentrations in the untreated ewes rose from 340 to 950 pmol/l in plasma and decreased in liver from 330 to 170 nmol/kg fresh tissue. In the Co treated group, vitamin B₁₂ concentrations in plasma rose from 500 to 1550 pmol/l and in liver from 310 to 560 nmol/kg fresh tissue. In the second year, vitamin B₁₂ concentrations in serum in the unsupplemented groups fell from 500 to 260 pmol/l around lambing before rising again to starting values at weaning, and liver vitamin B₁₂ concentrations fell from 450 at the start to 230 nmol/kg fresh tissue at the end of the trial. Serum vitamin B₁₂ concentrations in the two-tooth supplemented group rose from < 500 to > 3000 pmol/l whereas in the four-tooth supplemented group serum vitamin B₁₂ levels started at ~2800 and rose to nearly 5000 pmol/l. The supplemented four-tooths maintained higher liver vitamin B₁₂ concentrations throughout compared to the supplemented two-tooths, viz 680 compared to below 400 at the start and 900 versus 650 nmol/kg fresh tissue at weaning, respectively. MMA in the untreated groups rose to 15 and to 8 µmol/l during early lactation in the first and second years, respectively, whereas MMA in the treated groups stayed below 3 µmol/l in the first season and below 1.5 µmol/l in the second season. There was a live weight response to treatment in the ewes as the unsupplemented groups showed a significantly lower weight gain during the trials than the supplemented groups, viz 10.0 versus 13.6 kg in the first year, and 10.6 versus 13.3 kg in the four-tooths and 9.9 versus 12.1 kg in the two-tooths in the second year. There was also a significant difference in faecal egg count (FEC) in the first year. FEC in the untreated group was higher during lactation than in the treated group, viz 590 versus 170 eggs per gram wet faeces (epg), respectively. In the second year, the two-tooths had a higher FEC than the four-tooths, viz 120 versus 40 epg during the same time span, respectively. While there was a trend for treatment having an effect on FEC similar to that in the first year it was not significant. Supplementation of ewes in the first year increased mean milk vitamin B₁₂ concentrations at lambing from 800 to 1400 pmol/l and at weaning from 1750 to 4000 pmol/l. In the second year, Co bullet treatment increased milk vitamin B₁₂ concentrations in the four-tooths and two-tooths from 1500 and 2300 to 4000 and 2900 pmol/l at lambing, and from 1800 and 1400 to 6200 and 4500 pmol/l at weaning, respectively. Treatment of ewes increased vitamin B₁₂ concentrations in the lambs which were not themselves supplemented. Plasma values in the first year increased from 160 to 325 pmol/l soon after birth and from 650 to 900 pmol/l at weaning, and liver values from 75 to 140 nmol/kg fresh tissue soon after birth and from 150 to 240 nmol/kg fresh tissue at weaning. In the second year, plasma vitamin B₁₂ concentrations increased from 160 to 380 pmol/l soon after birth and from 500 to 700 pmol/l at weaning, and in liver from 130 to 260 nmol/kg fresh tissue soon after birth and from 220 to 340 nmol/kg fresh tissue at weaning. There was also a significant effect of ewe supplementation on lamb MMA in 1997/1998 when values decreased from 19 to 8 µmol/l around the time of rumen development. MMA in the second year stayed below 3 µmol/l throughout in all groups of lambs. There was no difference in LWG between any groups of lambs. FEC was lowest in the group where both ewes and lambs were supplemented and highest in the group where neither ewes nor lambs were treated. Further investigations were conducted on farms in Southland with lambs post-weaning in order to compare changes in serum and liver vitamin B₁₂ with serum MMA and LWG to determine the critical time and level of deficiency. In the first year, three farms with 50 lambs each participated. Lambs from each farm were allocated to five groups of 10 animals each. The first group received a Co bullet at weaning, and each month another group was treated with a Co bullet. The lambs were weighed monthly, and blood and liver samples were taken prior to treatment and each subsequent month from five lambs of the first supplemented group. The trial lasted about four months. Serum vitamin B₁₂ concentrations in lambs at weaning were between 500 and 1000 pmol/l. Although supplementation increased serum levels for the first month this was followed by a drop to near or below starting concentrations. An exception was Farm 3 where serum vitamin B₁₂ concentrations rose again at the end of the trial. Liver vitamin B₁₂ concentrations also showed an overall decline from starting levels (200 to 300 nmol/kg fresh tissue) to the end of the trial (100 to 200 nmol/kg fresh tissue). MMA started around 2 µmol/l and reached between 6 and 7 µmol/l in the untreated lambs on Farms 1 and 3 two months after weaning before decreasing to around 3 µmol/l at the end of the trial, whereas the treated lambs maintained MMA concentrations around 2 µmol/l. On Farm 2 MMA started just below 5 µmol/l, decreased to around 1 µmol/l for treated and untreated lambs one month later and rose again to between 2.5 and 4 µmol/l, respectively, at the end of the trial. LWG was below average for all lambs (between 0.20 and 0.04 kg/d except for Farm I in the first month after weaning) but no significant differences were noted between treated and untreated lambs on any of the farms. Another trial was conducted on one of these farms in the following year. One hundred lambs were divided into two groups of 50 each at weaning and sampled monthly for about six months. One group was treated with two Co bullets, the other group remained untreated. Pasture Co was between 0.04 and 0.07 µg/g DM, yet serum levels for the untreated group stayed ~500 pmol/l throughout the trial. Serum vitamin B₁₂ concentrations for the treated group started at ~500 pmol/l, rose to ~2500 pmol/l before falling back to ~2000 pmol/l. Liver vitamin B₁₂ concentrations for the untreated and treated groups were 529 and 427 nmol/kg fresh tissue at weaning, respectively. This decreased for both groups to ~350 nmol/kg fresh tissue one month after weaning. In the untreated lambs liver values decreased further to ~290 nmol/kg fresh tissue whereas they increased to ~450 nmol/kg fresh tissue for the treated group at the end of the trial. MMA concentrations started between 2 and 3 µmol/l for both groups and increased to 4.5 µmol/l for the untreated group one month later before falling back to 3.2 µmol/l. In the treated group MMA decreased to ~1µmol/l and stayed at that level throughout the trial. There was no difference in weight gain. In order to obtain an understanding of the distribution of corrinoids in biological tissues a High Performance Liquid Chromatography method was developed. The sensitivity of the analytical method meant that it was only practical to assay mainly liver samples because of the higher vitamin B₁₂ concentrations than in other tissues. The general finding was that the coenzyme 5’ –deoxyadenosylcobalamin (ado-cbl) constituted the highest proportion of corrinoids in liver (45 %), followed by analogues (28 %), OH-cbl (24 %) and lastly methy1cobalamin (3 %). Ado-cbl did tend to be proportionately higher in supplemented than in unsupplemented animals (56 and 42 %, respectively), whereas biologically non-active analogues tended to be higher in untreated than in treated sheep (29 and 21 %, respectively). It was concluded that in the farm trials Co deficiency was only mild or not present although deficiency would have been predicted from the low vitamin B₁₂ concentrations in serum and liver and from raised MMA values. Therefore, currently used thresholds in New Zealand appear to be too high for vitamin B₁₂, and overseas values for MMA do not seem to be appropriate. Revised marginal ranges of 100 to 200 pmol/l for serum, 100 to 200 nmol/kg fresh tissue for liver and 10 to 20 µmol/l for MMA are suggested. Further, this work shows that Co bullets were effective in elevating blood and liver vitamin B₁₂concentrations for longer than one year. In the trials with preruminant lambs it was found that maintenance requirements were met by the vitamin B₁₂ content of milk replacer. There is evidence from indoor and farm trials that vitamin B₁₂ from milk was much more readily absorbed than vitamin B₁₂ from supplements. It was estimated that suckling lambs probably require between 1200 and 4000 pmol vitamin B₁₂/d, depending on age.

Realizou-se um estudo descritivo conduzido no Hospital de Clínicas de Porto Alegre, no período de outubro de 2001 a outubro de 2002, com pacientes HIV positivos e anêmicos. Objetivo: avaliar a associação entre anemia e o perfil nutricional em uma amostra de pacientes HIV+ . Métodos: Foram incluídos 34 pacientes maiores de 18 anos, sendo todos pacientes diagnosticados com anemia. Foram analisados exames laboratoriais, avaliação da ingestão alimentar (recordatório alimentar de 24h), freqüência alimentar e coleta dos parâmetros antropométricos dos pacientes. Resultados: O recordatório alimentar de 24 horas demonstrou a deficiência na ingestão de folato pela maioria dos pacientes; enquanto que vitamina B12 e ferro estiveram de acordo com as RDA’s. Conclusão: A causa da anemia nestes pacientes talvez não tenha sido em função desta deficiente ingestão de folato, ainda mais por se tratar de um estudo descritivo. Ressalta-se a importância do profissional para o acompanhamento nutricional destes pacientes, para a promoção de um adequado estado nutricional e qualidade de vida. / Was develop a descritive study conducted in the Porto Alegre Clinics’ Hospital, from october 2001 to October 2002, with positive HIV and anemics patients. Objectives: Evaluate the association between anemia and nutritional status in positive HIV patients. Methodology: Were included 34 patients under 18 years old, all diagnosed with anemia. Laboratorial exams, evaluation of food intake (24h register), questionary of food intake frequence and anthropometrics data were collected. Results: The 24h register food intake showed the deficiency in folate intake by most of patients ,while vitamine B12 and iron intake were accorded RDA’s. Conclusions: The etiology of anemia in these patients maybe wasn´t cause by this folate deficiency, also because this was a describe study. The presence of a professional appears to be important for the nutritional treatment of these patients, to develop a health nutrional status and quality of life.