The project involved the study of 12 Victorian municipal wastewater treatment plant discharges. These included lagoon-based plants and those with activated sludge based processes. Permission was obtained from all the relevant water authorities to collect samples of final effluent at point of discharge to the environment, whether that was to a creek, a river, the ocean, or the land. Samples were collected in November 2003, and then again in April and June 2004, and subjected to a number of biological and chemical analyses, including toxicity tests, measurement of hormonal (estrogenic) activity using yeast-based bioassays, and the measurement of specific hormonal concentrations (17-estradiol) using enzyme-linked immunosorbent assays (ELISA). Almost all of the effluents examined showed estrogenic activity, to a greater or lesser extent (no response to 55 ng/L 17β-estradiol equivalents). On the whole, the levels of estrogenic activity observed were to the lower end of the range observed overseas in the northern hemisphere, and comparable with that recently reported in Australia and New Zealand using similar, human-estrogen receptor based assays (no response to ~ 10 ng/L 17β-estradiol equivalents). The reassuring low/no assay response is bolstered by the chemical assessment of estradiol concentrations by ELISA, which returned concentrations of these compounds for the most part in the range 2-5 ng/L. From an aquatic environmental perspective, it is difficult to say with any certainty what the potential risk to aquatic organisms in waters receiving these effluents will be. Typically, in environmental risk assessment one first looks to agreed national or international guideline or trigger values for the type of waters being assessed. In this case, there are as yet no guideline values. Without guideline values to drive the assessment, then one compares a chemical’s concentration in a sample (in this case a WWTP effluent) with data obtained from toxicological experiments in which the concentration known to elicit a specific effect has been determined. In this case, levels of 17β-estradiol were typically between the lowest reported level to induce the production of Female-indicative proteins in male fish (plasma vitellogen; 1 ng/L), and the lowest concentration of known to induce intersex in fish (8 ng/L). Consequently, such levels in a WWTP discharge are likely to be an environmental risk if there is little or no dilution of the discharge by the receiving water, i.e. discharge represents major component of stream flow. In short, to truly assess the risk (hormonal impact) of these WWTP effluents, in vivo testing needs to be undertaken, ideally with a representative native species but failing that with a ‘standard’ species such as the fathead minnow. When this programme began, the ‘watching brief’, being held in Australia on the topic of endocrine disrupting chemicals and their potential effects on aquatic wildlife was considered too passive by many. It still is, by some. Despite the assurance the results may provide (of minimal impact in most cases if there is significant dilution), there is still a need for further extensive on-ground, reassurance research to provide data for higher-level risk assessment by industry and government agencies.

<p>The presence of human pathogens in water indicates the sanitary risk associated with different types of water utilization. This study surveyed the sources of human pathogens in urban waters. In order to evaluate the microbiological water quality of urban water, the enumeration of various indicator bacteria (total coliform, fecal coliform, E.coli and enterococci) is usually used.</p><p>The abundance of indicator bacteria in urban water indicates the level of fecal contamination and the presence of other human pathogens such as protozoan pathogens (Giardia lamblia & Cryptosporidium parvum).</p><p>Fecal pollution of urban waters can be from human and animal origin. Point sources of fecal contamination in an urbanized area are the effluents of urban wastewater treatment plants. While non-point sources are usually originated from diffuse sources such as (runoff from roads, parking lots, pets, leaks, failing septic systems and illegal sewer connections to storm drains). urban stormwater is considered as a major carrier for delivering human pathogens from diffuse sources to receiving waters. Increases in urban stormwater volumes have resulted from increasing urbanization and growth of impervious surfaces.</p><p>In order to reduce high amounts of human pathogens in urban waters, different methods are used nowadays to develop urban wastewater treatment plants technologies and urban stormwater management practices.</p>

<p>Målet med den här studien är att förhindra effektivitetssänkningar i moderna biologiska reningsanläggningar för skogsindustriella avloppsvatten. Biologiska processer är vanligtvis effektiva när det gäller att reducera lättnedbrytbart organiskt material. Eftersom den utgörs av levande mikroorganismer (MO) är tekniken känslig. Toxiska ämnen kan döda en kultur av MO. Innan de har ökat i antal igen kommer reduktionseffektiviteten att vara påverkad för en ansenlig tid framöver. I ett pappers- och massabruk finns många typer av vätskor som oavsiktligt kan hamna i reningsanläggningen. I den här studien har svartluts inverkan på reduktionsgraden studerats.</p><p>Biologiska reningsverk har använts under en lång tid. De har utvecklats från att vara generella reningsverk till att bli konceptanläggningar i flera steg, där varje steg har designats för sitt eget syfte med specifika MO. I det här arbetet har ett MultiBio-koncept vid Gruvön Bruk studerats. En anläggning i laboratorieskala har byggts för svartlutsförsöken. Resultatet har jämförts mot simulerade värden och värden från bruket.</p><p>När ett spill av svartlut passerar genom en MultiBio späds det ut mellan varje steg. Halten av svartlut är hög i de första stegen vilka påverkas kraftigt. I de första stegen lever snabbväxande organismer som återhämtar sig på ett par dagar. De mer känsliga aktivslamstegen finns längre bak i reningsverket och är skyddade från toxiska koncentrationer av svartlut. Endast vid försök med 24-timmarstillsatser blev koncentrationen av svartlut så hög att den påverkade MO negativt.</p><p>Den biologiska processen i ett reningsverk kan störas om MO förnekas något essentiellt behov. En biologisk process har ett flertal behov, bland annat behöver aeroba MO löst syre. Eftersom syresättning av vattnet är energikrävande och kostsamt är det inte problemfritt att skapa en omgivning som ger hög effektivitet samtidigt som man strävar efter att sänka energiförbrukningen. I avhandlingen beskrivs ett tillvägagångssätt för att studera energieffektiv syresättning.</p> / <p>The aim of this study is to prevent efficiency reduction in modern wastewater treatment plants for forest industry effluents. Biological processes are usually efficient in reducing biodegradable organic material. Since the technique depends on living microorganisms (MO) it is sensitive. Toxic substances can kill the population. It takes considerable time for the MOs to grow in number and the treatment efficiency will be affected accordingly. In a pulp and paper mill, a number of liquors are handled that can reach the treatment plant by accident. In this study the impact of black liquor spills on treatment efficiency has been studied.</p><p>Biological treatment methods have been used for a long time. They have developed from general treatment plants to multiple stage concepts, where each stage is designed for its own purpose with specific MO cultures. In this thesis, a plant with the MultiBio concept, located at Gruvön Mill in Sweden has been studied. A laboratory scale MultiBio plant has been constructed for the trials in which efficiency during black liquor exposure has been measured. The measured laboratory results were evaluated by comparing them with simulated values and a mill case.</p><p>When a shock of toxic black liquor passing through a MultiBio concept, the black liquor is diluted between the compartments. The first compartments that are exposed to high concentrations of black liquor are affected negatively. The MOs in the first compartment are fast growing and recover in a few days. The more sensitive activated sludge compartments are located further on in the plant. A toxic concentration is found in the activated sludge compartments only when the duration of the spill is 24 hours or more.</p><p>Denying the MOs their needs can disturb biological treatments. Among many things, a biological process needs dissolved oxygen. Since aeration is energy consuming and expensive, there is a conflict between gaining high efficiency and, at the same time, decreasing the energy consumption. In this study, an approach to saving energy for aeration is initiated.</p>

<p>An analysis of a specific application of anaerobic wastewater treatment at a pulp and board mill, Stora Enso Skoghall, was carried out. A literature study was done and effluent wastewater from CTMP pulp production was analyzed. The analysis consisted of a broad constituent analysis and an anaerobic treatment trial. Results from these test where used to calculate the treatment effectiveness and resulting energy potential at the mill.</p><p>The literature study and constituent analysis showed that toxicity of the water to be treated, due to wood extractives and sulphur content, was of concern. Detoxification chemicals targeting these constituents where used in the anaerobic treatment trial. The trial consisted of a two chamber upflow reactor and aerobic post treatment. The result of the trial is that the water of concern has about 50% anaerobically degradable content. A full scale implementation of anaerobic wastewater treatment would result in a methane production of 3,76 to 7,76 GWh/yr. An application of anaerobic wastewater treatment will also result in electricity savings, in aerated pond, and increase the capacity margin on current sludge handling techniques.</p>

<p>Hedesunda reningsverk är beläget i södra delen av Gävle kommun och hanterar avloppsvatten från Hedesunda samhälle och ett fåtal närbelägna byar. Antalet anslutna personer är strax över 1500. Reningsverket byggdes på 1960-talet och byggdes om i slutet av 1990-talet. Vid ombyggnaden anlades torkbäddar för avvattning av det slam som produceras vid reningsverket. Inledningsvis fungerade dessa torkbäddar bra men sedan 2005 har slammet i bäddarna inte avvattnats i önskad omfattning.</p><p> </p><p>Syftet med detta examensarbete var att utreda hur slamavvattningen i Hedesunda ska se ut i framtiden. Utgångspunkten var att den nuvarande lösningen är otillräcklig och att en förändring är nödvändig. Inledningsvis delades frågeställningen upp i två separata delar: avvattning av slammet och avsättning för avvattnat slam. Fokus låg på ekonomiska och miljömässiga aspekter av slamhantering och en sammanvägning av dessa gjordes där så var möjligt.</p><p> </p><p>Frågan om hur avvattningen bör se ut undersöktes genom att en enkät skickades ut till nio olika företag som levererar avvattningsutrustning. Dessa fick svara på 17 frågor om vilken lösning de ansåg vara bäst lämpad för reningsverket i Hedesunda. Sammanlagt samlades sex olika svar in. Utöver dessa undersöktes även alternativet att restaurera de befintliga torkbäddarna närmare. Detta skedde bland annat genom provtagning och analys av slammet i bäddarna. De olika alternativen för slamavvattning sammanställdes och jämfördes genom att varje undersökt parameter delades in i intervall som gav olika betyg. De tre alternativ som enligt denna undersökning ansågs bäst lämpade var att restaurera de befintliga bäddarna, att anlägga vassbäddar eller att installera en mindre silbandpress.</p><p> </p><p>Då slammet avvattnats återstår frågan kring hur det avvattnade slammet ska hanteras. Detta undersöktes genom att två alternativ studerades närmare. Dessa var att fortsätta med den nuvarande hanteringen som är kompostering och tillverkning av anläggningsjord eller att certifiera slammet genom REVAQ och sprida det på åkermark. Ett försök till att skatta kostnaden för de båda alternativen gjordes, bland annat genom att frågor ställdes till de kommuner/driftbolag som idag är certifierade enligt REVAQ. Kostnaden för certifiering och spridning på jordbruksmark är betydligt mycket högre än kostnaden för kompostering. Certifieringsalternativet innebär dock en större måluppfyllelse i och med att fosfor återförs till produktiv mark.   </p><p> </p><p>Resultatet av studien är ett konkret förslag på hur slamavvattning och avsättning för slam vid Hedesunda reningsverk kan skötas i framtiden. Detta förslag innebär att de nuvarande torkbäddarna restaureras och att möjligheterna att certifiera flera delar av Gästrike Vatten AB:s verksamhet utreds närmare. Att enbart certifiera slam från Hedesunda reningsverk anses ekonomiskt orimligt.</p> / <p>Hedesunda wastewater treatment plant (wwtp) is located in the southern part of Gävle municipality in Sweden. The wwtp treats wastewater from Hedesunda community and a few nearby villages. All in all there are approximately 1500 persons connected to the plant. Hedesunda wwtp was originally built in the 1960’s and was restored in the late 1990’s. At the restoration sludge drying beds were built on the site for sludge dewatering. Initially, these beds worked fine but since 2005 the dewatering result has not been satisfactory.</p><p> </p><p>The objective of this master thesis has been to investigate different alternatives for a more effective sludge handling at Hedesunda wwtp in the future. Initially the sludge handling process was divided into two different parts: sludge dewatering and management of dewatered sludge. The focus has been on economical and environmental aspects of sludge handling and where it’s been possible these factors have been weight together.</p><p> </p><p>The question of how a more effective dewatering of sludge could be achieved was answered by a questionnaire that was sent to different companies that supply dewatering equipment to the Swedish market. Altogether six answers were collected. Besides these answers the option of restoring the existing sludge drying beds was also considered. The latter was done by sampling and analyzing sludge in the existing beds, among other things. The different options for dewatering were compiled and compared in a matrix where every parameter was split up in intervals and assigned grades. Three options that received the highest total grade were considered the best options. These were: restoring the sludge drying beds, reed beds and a small belt filter press.</p><p> </p><p>The matter of how to handle the dewatered sludge was simplified to a study of only two alternatives. These were to continue the present handling or to certify the sludge according to REVAQ and use it as fertilizer. Attempts to estimate the cost for the two alternatives were made. The cost for certification is higher than the cost for composting, but to certify the sludge will lead to a higher target achievement.</p><p> </p><p>The study resulted in concrete suggestions for how the sludge at Hedesunda wwtp can be handled in the future. This suggestion states that the existing sludge drying beds should be restored and that the possibility to certify several wwtp:s within Gästrike Vatten AB should be investigated. It is not considered economically plausible to certify sludge from Hedesunda wwtp alone.</p>

In this thesis, the application of molecular microbiology methods to understand wastewater treatment bio-reactions is described. Two different wastewater treatment systems were chosen for the experimental work. Firstly; the activated sludge processes at two different facilities in Sweden (Västerås and Eskilstuna) were investigated and compared in a context where low temperatures can affect the efficiency of the nitrogen removal performance in terms of nitrification. Initially, fluorescence in situ hybridization (FISH) was utilised in order to quantify some of the species involved in ammonia and nitrite oxidation at Västerås, providing information on how the different communities react to decreasing temperatures. Then, the polymerase chain reaction (PCR), cloning-sequencing method was employed in order to study the composition of the ammonia oxidizing bacteria (AOB) community at the same two wastewater treatment plants (WWTPs). Secondly; the potential use of constructed wetlands for the treatment of winery wastewater was studied. High ethanol concentration artificial wastewater with and without inorganic nutrients (nitrates and phosphates) was fed in a set of pilot-scale constructed wetlands. Pollutant removal performance and enzyme activity tests were carried out. Additionally, the bacterial community structure was investigated by means of denaturing gradient gel electrophoresis (DGGE). In the first set of studies it was shown that the AOB population which plays a major role in nitrifying reactors presented a seasonal shift and a higher diversity at Västerås during winter time, while the nitrification performance maintained stable levels and the ammonia removal efficiency increased. Thus, the higher ammonia removal efficiency at Västerås could be related to the diversity of the AOB population composition. Lastly, when constructed wetlands were in focus, the differential effects of ethanol and nutrients over the chemical oxygen demand (COD) removal performance were proven. In fact, the addition of nutrients on one of the experimental wetlands increased the COD (ethanol) removal and supported the maintenance of a bacterial population similar to the control wetland (no ethanol added). In conclusion, both studies proved a strong relationship between process performance (pollution removal) and the dynamics of the bacterial communities involved.

Research performed at the Division of Environmental Microbiology has over the last years resulted in the isolation of possible bacterial key-organisms with efficient nutrient removal properties (Comamonas denitrificans, Brachymonas denitrificans, Aeromonas hydrophila). Effective use of these organisms for enhanced nutrient removal in wastewater treatment applications requires the strains to be retained, to proliferate and to maintain biological activity within theprocess. This can be achieved by immobilization of the organisms using an appropriate system.Two putative immobilization systems, agar entrapment and biofilm formation, wereassessed. Surface attached biofilm growth provided better results with respect to cell retention,proliferation and microbial activity than immobilization in agar beads. Thus, biofilm physiology was further characterized using simplified systems of single, dual or multi strain bacterial consortia containing the key-organisms as well as other wastewater treatment isolates. Mechanisms for initial adherence, biofilm formation over time, dynamics and characteristics of extracellular polymeric substances (EPS) and exopolysaccharides, nutrient removal activity as well as the effect of bacterial interactions were investigated. The results showed that all theassessed bacterial strains could form single strain biofilm providing that a suitable nutrientsupply was given. Production of EPS was found to be critical for biofilm development and both EPS and polysaccharide residue composition varied with bacterial strain, culture conditions and biofilm age. Denitrification and phosphorus removal activity of the keyorganisms was maintained in biofilm growth. Co-culturing of two or more strains resulted in both synergistic and antagonistic effects on biofilm formation as well as the microbial activitywithin the biofilm. Bacterial interactions also induced the synthesis of new polysaccharideswhich were not produced in pure strain biofilms.The complexity of single and mixed strain biofilm development and the implications of interactions on biofilm performance were underlined in this study. The data presented can be useful for modeling of biofilm systems, serve as a tool for selection of bacterial strain combinations to use for bioaugmentation/bioremediation or provide a base for further experiment design. / QC 20100622

As part of a research project aiming to develop and evaluate a hydroponic system for wastewater treatment in Sweden, extended nutrient removal by microalgae was tested. The hydroponic/microalgal wastewater treatment system was built in a greenhouse in order to improve growth conditions for plants and algae. Studies on the treatment step with microalgae showed that phosphorus removal could be successfully accomplished owing to the cmbined effect of phosphorus assimilation and biologically mediated chemical precipitation of calcium phosphates. This precipitation was mainly induced by the increased pH in the algal cultures, and the pH increase was in turn a result of the inorganic carbon assimilation by the algae. The results showed that the algal growth was mainly light limited which resulted in higher algal biomass density and also lowe residual nutrients in the water at longer hydraulic retention times (HRT). In contrast the phosphorus removal rate was load limited, i.e. shorter HRT gave higher removal rates. This load dependency was due to the chemical precipitation, whereas the phosphorus assimilation was dependent on algal growth. Furthermore, results from an intensive study during summer showed that culture depths of 17 cm gave higher removal efficiencies (78% - 92%) than cultures of 33 cm (66% - 88%). On the other hand, the removal rate per area was higher in the deeper cultures, which implies that these may be preferred if area is of concern. Nitrogen removal was achieved mainly by the assimilation of nitrate to algal biomass, and removal efficiencies of around 40% (nitrate) could be reached for most parts of the year although the nitrogen removal performance was quite uneven. Up to 60% - 80% could however be reached during summer in the shallow cultures. A net removal in total nitrogen of up to 40% was observed in the shallow cultures during summer, which was most probably a consequence of grazing zooplankton and subsequent urea excretion and ammonia volatilisation as a reslt of the high pH values. Over the year, there were large fluctuations in algal growth and removal efficiency as a result of the seasonal variations in light and tempeature. During winter, phosphorus removal efficiencies lower than 25% were observed in the shallow tanks and lower than 10% in the deep tanks. Additional illumination during winter improved the phosphorus removal in the shallow cultures but did not have a significant efect on the deep cultures. Such additional illumination increases the total energy demand of the system, and hence alternative methods for phosphorus removal during winter would probably be more economical unless the algal biomass roduced had great commercial value. / QC 20100907

Anaerobic digestion of excess biological wastewater treatment sludge (WAS) from pulp mills has the potential to reduce disposal costs and to generate energy through biogas production. The organic matter in WAS is highly structured, which normally hinders biogas production. This study investigated three methods of pretreating WAS from two different pulp mills before anaerobic digestion to improve biogas yield and production rate. The three pretreatment methods tested were: i) thermal pretreatment at 170oC, ii) caustic pretreatment at 140oC and pH 12, and iii) sonication at 20 kHz and 1 W/mL. Thermal pretreatment proved to be the most effective, increasing biogas yield by 280% and 50% and increasing production rates 300-fold and 10-fold for the two samples, respectively. Caustic pretreatment showed similar results, but resulted in the formation of soluble non-biodegradable compounds. Sonication was the least effective pretreatment and did not substantially increase biogas yield, but increased biogas production rate.

Anaerobic digestion of excess biological wastewater treatment sludge (WAS) from pulp mills has the potential to reduce disposal costs and to generate energy through biogas production. The organic matter in WAS is highly structured, which normally hinders biogas production. This study investigated three methods of pretreating WAS from two different pulp mills before anaerobic digestion to improve biogas yield and production rate. The three pretreatment methods tested were: i) thermal pretreatment at 170oC, ii) caustic pretreatment at 140oC and pH 12, and iii) sonication at 20 kHz and 1 W/mL. Thermal pretreatment proved to be the most effective, increasing biogas yield by 280% and 50% and increasing production rates 300-fold and 10-fold for the two samples, respectively. Caustic pretreatment showed similar results, but resulted in the formation of soluble non-biodegradable compounds. Sonication was the least effective pretreatment and did not substantially increase biogas yield, but increased biogas production rate.