Next-generation batteries with improved microstructure and performance are on their way to meet the market demands for high-energy and power storage systems. Among different types of batteries, Li-ion batteries remain the best choice for their high energy density and long lifetime. There is a constant but slow improvement in Li-ion batteries by developing new materials and fabrication techniques. However, further improvements are still needed to meet government and industry goals for cost, cycling performance, and cell lifetime. A fundamental understanding of particle-level interactions can shed light on designing new porous electrodes for high-performance batteries. This is a complex problem because electrodes have a multi-component, multi-phase microstructure made through multiple fabrication processes (i.e., mixing, coating, drying, and calendering). Each of these processes can affect the final microstructure (particle and pore locations) differently. This work seeks to understand the porous microstructure evolution of Li-ion electrodes during the drying and calendering fabrication processes by a combination of modeling and experimental approaches. The goal is to understand the mechanisms by which the electrode components and fabrication processes determine the battery microstructure and subsequent cell performance. A multi-phase smoothed particle (MPSP) model has been developed on a publically available simulation platform known as LAMMPS. This model was used to simulate particle-level interactions and predict the mechanical and transport properties of four fabricated electrodes (i.e. a graphite anode and three traditional metal oxide cathodes). One challenge was to include different electrode components and their interactions and relate them to physical properties like density and viscosity that can be measured experimentally. Another challenge was to generate required electrode property data for model validation, which in general was not found in the literature. Therefore, a series of experiments were conducted to provide that information, namely slurry viscosity, electronic conductivity, porosity, tortuosity, elastic modulus, and electrode crosssections. Understanding these properties has value to the battery community independent of their use in this study. The MPSP model helps us explain observed transport heterogeneity after calendering but brings up new questions about the drying process that have not been addressed in previous works. Therefore, the drying fabrication step was studied experimentally in more detail to fill this knowledge gap and explain our simulation results. The MPSP model can also be used as a predictive tool to explore the design space of Li-ion electrodes where conducting the actual experiments is very challenging. For example, the distinct effect of particle size, shape, orientation, and stiffness on electrode transport and mechanical properties are difficult to determine independently, and therefore this model is an ideal tool to understand the effect of these properties. The final model, which is publically available, could be used with adjustments by future workers to test new materials, fabrication processes, or electrode design (e.g., a multi-layered structure).

M. Tech. (Department of Metallurgical Engineering, Faculty of Engineering and Technology), Vaal University of Technology. / Elemental titanium (Ti), niobium (Nb), zirconium (Zr), and iron (Fe) powders were used to fabricate four near-β alloys with non-toxic of composition Ti-34Nb-25Zr, Ti-34Nb-25Zr-0.4Fe, Ti-34Nb-25Zr-1.2Fe, and Ti-34Nb-25Zr-2Fe (wt. %) (TNZ and TNZF) using spark plasma sintering (SPS) of nano-crystalline powders attained by high energy ball milling. The fabricated alloys were compared to Ti-34Nb-25Zr (used as a benchmark alloy in this study) and comparison was made with the commercially used Ti base alloys produced either by conventional methods or powder metallurgy. The powder mixtures were milled for 5 hours using a Simoloyer high energy ball mill with a ball to powder ratio of 10:1 and a rotational speed of 1000 rpm. This was followed by sintering the mechanically alloyed powders at 1100 ºC for 10 minutes with a pressure of 50 MPa and a heating rate of 100 ºC/min using an H-HP D25 spark plasma sintering furnace (FCT System, Germany). The powders were characterised for particle size and crystal structure using SEM and XRD. The consolidated components were characterised with regards to density, microstructure, mechanical properties. The electrochemical behaviour of the alloys was investigated using a Digi Ivy DY2300 series potentiostat. Three corrosion medium, Sodium chloride (NaCl), phosphate buffered saline solution (PBS) and Dulbecco’s modified eagle’s medium that mimic the conditions in the human body were used. Mouse myoblast cell line (C2C12) was used to investigate the biocompatibility of the sintered alloys in 1010x5 mm specimens using standard colorimetric assay MTT. Both electrochemical and biocompatibility test were conducted in triplicates and the results compared with that of the benchmark. Results of mechanical alloying of powder mixtures demonstrated an inhomogeneous structure. Milling for 5 hours resulted in agglomeration of small Fe and Zr particles. Milling for 3 hours resulted in a better distribution of elements compared to longer milling times. Therefore, sintering powders milled for 3 hours would have yielded better results. The densification results were acceptable and ranged between 97-99% of theoretical densities. Although some porosity was observed, especially on the un-etched microstructure. An insignificant decrease in density was observed when 1.2 (wt. %) Fe was added. The sintered samples had microstructures which were not homogenous. However, the addition of Fe yielded a more homogeneous microstructure compared to the one with less Fe. Therefore, TNZF with 2 (wt. %) Fe had a more homogenous microstructure. Sintering at 1100 ºC resulted in undissolved niobium and titanium which were observed in the microstructure as dark and white areas. The hardness of the TNZF alloys were comparable and lied between 373 and 432 Hv. These hardness values are higher than other similar titanium-based alloys fabricated using conventional methods. The addition of Fe to TNZ showed an insignificant decrease in hardness. The addition of Fe was found to decrease the Young’s Modulus of TNZ from 119.1 to 80 GPa with an addition of 2 wt.% Fe. However, an unacceptable reduction (230.91 to 158.2 MPa) in strength was also noticed. Pseudo passivation was observed when the alloys were immersed in 0.9 % Sodium Chloride (NaCl) which could be attributed to the inhomogeneity in the microstructure. The possibility of pitting corrosion was also observed. The alloy containing 2 Fe (wt.%) was found to be more corrosion resistant than the other alloys. The TNZF alloys exhibited better corrosion resistance in 0. 9% NaCl compared to phosphate buffered solution (PBS) and DMEM. The corrosion behaviour in PBS and DMEM cannot clearly be explained from the graphs. The morphology of the corroded samples was almost the same for all the alloys in different corrosion media. The microstructures showed pits which could have been from the pores that acted as initiation sites for pitting. In cell culture for 1 and 7 days, the cell viability for TNZF alloys was greater than that of the control group (TNZ). A significant decrease in cell viability for TNZF was observed in cell culture for 4 days. The addition of Fe on TNZ do not cause toxic effects and show good cell adhesion, indicating in-vitro cytocompatibility. The greatest cell viability of 102±3.0 % for Ti-34Nb-25Zr-2Fe. The analysis of cell morphology indicated good cell-substrate interaction. The TNZF alloys developed in this study can be suitable candidates for orthopaedic implant application due to their low Young’s modulus, corrosion resistance and superior biocompatibility. However, the strength needs significant improvement. The advantage of this biomaterial, when compared to commercial alloys, is the absence of cytotoxicity elements such as Al and V.

No description available.

Poly(N-isopropyl-acrylamide) (PNIPAM) is a thermoresponsive polymer with a wide range of biological applications, including drug delivery, biosensing, and tissue engineering. The tunability of the structural and mechanical properties of PNIPAM makes it particularly at- tractive in emulating cell environments and dynamic cytoskeletal deformations. This thesis discusses PNIPAM's properties and applications in different forms i.e., solution, brushes, hydrogels, and surface patterned hydrogels, with specific focus on lithographically patterned substrates coated with PNIPAM films. The scaffolds are investigated for structural and me- chanical responses to thermally driven changes in the PNIPAM hydration states using atomic force microscopy (AFM). AFM measurements on our lithographically patterned substrates show that the substrate pattern and coating method enable the fabrication of scaffolds with different topographic and mechanical properties across a wide thermal range. Importantly, these scaffolds exhibit variations in both lateral topography and Young's modulus, rendering them well suited for investigations of differential mechanical stresses experienced by cells and cell membranes. / Master of Science / Poly(N-isopropyl-acrylamide) (PNIPAM) is a polymer which can change its water absorption depending on the temperature of its aqueous environment. It transitions from a swollen state at room temperature to a collapsed state at around 32 °C. These thermally tunable properties make PNIPAM an attractive candidate in a variery of applications, including biomedical and biophysical applications. In this thesis, PNIPAM is coated on lithographically patterned substrates to emulate the cellular cytoskeleton. Atomic force microscopy (AFM) measurements are performed to measure the topography and mechanical properties of the fabricated scaffolds. The results show that the coating method and the features of the used substrate allow the fabrication of different surface topographies with biologically relevant mechanics.

This study demonstrates that the surface motility of the bacterium Pseudomonas aeruginosa is dependent on the surface chemistry of the underlying substrate. In particular, cells on hydrophobic polydimethylsiloxane (PDMS) have a speed that is on average 38% greater than on hydrophilic PDMS. These results were obtained using time-lapse microscopy of bacteria exposed to continuously flowing tryptic soy broth growth medium at 37 ⁰C. Not only are the mean speeds different, the distributions of speeds are also different: on the hydrophobic substrate, a smaller proportion of bacteria move by less than about one body-length (~3 µm) in 60 minutes. In addition, the surface chemistry affects the orientation of the cells: there is a greater fraction of "walking" bacteria on the hydrophobic surface. Sensitivity to the substrate surface chemistry occurs despite the presence of a complex mix of substances in the growth medium and offers hope that surface chemistry can be used to tune motility and the progression to biofilm formation. Additionally, the effect of reducing the near-surface Young's modulus of the PDMS from 7000 to 70 kPA is investigated. For the lower modulus material, there is an increase in the likelihood of a bacterium executing sudden, high angle turns. This is evident in images with a framerate of one frame per 0.22s. However, the impact of these turns is averaged out over longer times such that the mean speed over periods of more than about one minute is the same for bacteria on both the high and the low modulus materials. Consequently, except over very short time intervals, Young's modulus in the surface region is not effective as a means of modulating motile behavior. / Master of Science / This study demonstrates that the ability of the bacterium Pseudomonas aeruginosa to move on a solid surface is dependent on the surface chemistry of the underlying substrate. In particular, cells on hydrophobic polydimethylsiloxane (PDMS) have a speed that is on average 38% greater than on hydrophilic PDMS. These results were obtained using time-lapse microscopy of bacteria exposed to continuously flowing growth medium at 37 ⁰C. Not only are the mean speeds different, the distributions of speeds are also different: on the hydrophobic substrate, a smaller proportion of bacteria move by less than about one body-length (~3 µm) in 60 minutes. In addition, the surface chemistry affects the orientation of the cells: there is a greater fraction of vertically-oriented bacteria on the hydrophobic surface. Additionally, the effect of reducing the stiffness of the PDMS from 7000 to 70 kPA is investigated. For the less stiff material, there is an increase in the likelihood of a bacterium executing sudden, high angle turns. This is evident in images with a framerate of one frame per 0.22s. However, the impact of these turns is averaged out over longer times such that the mean speed over periods of more than about one minute is the same for bacteria on both the high and the low stiffness materials. Consequently, except over very short time intervals, stiffness in the surface region is not effective as a means of changing patterns of surface-bound P. aeruginosa movement.

京都大学 / 新制・課程博士 / 博士(農学) / 甲第25326号 / 農博第2592号 / 新制||農||1105(附属図書館) / 京都大学大学院農学研究科森林科学専攻 / (主査)准教授 簗瀬 佳之, 教授 矢野 浩之, 教授 仲村 匡司, 教授 村田 功二 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DGAM

O conhecimento da resistência ao dano por choque térmico de materiais refratários é uma das características mais importantes para determinar seu desempenho em muitas aplicações, pois quando os refratários são submetidos a abruptas e severas variações de temperatura, estes podem sofrer danos. A resistência ao dano por choque térmico de um material pode variar com o tamanho de grão, com o tipo e valor da tensão, com a taxa de carregamento e com outras condições de aplicação da tensão, ou seja, não é uma propriedade intrínseca do material. Os métodos correntemente utilizados para prever o comportamento da resistência ao dano por choque térmico são baseados nos trabalhos de Hasselman, responsável pelo estudo da determinação dos parâmetros de resistência ao choque térmico, R (oC), o parâmetro de resistência ao dano por choque térmico R\'\'\'\' (m), e o parâmetro de estabilidade da trinca sob tensão térmica Rst (m1/2.oC). As equações previstas por ele levam em consideração o módulo de Young, o módulo de ruptura, a energia de fratura e o coeficiente de expansão térmica do material a ser analisado. A resistência ao dano por choque térmico pode ser avaliada por meio de ciclos térmicos, isto é, sucessivos testes de aquecimento e subseqüente resfriamento, com análise da queda do módulo de Young a cada ciclo. Para prever e avaliar a resistência ao dano por choque térmico, amostras de um concreto refratário comercial fornecido pela IBAR (Indústrias Brasileiras de Artigos Refratários), foram sinterizadas a 1000ºC e 1450ºC por cinco horas. Essas temperaturas foram definidas em virtude de o concreto em questão apresentar agregados de andaluzita, que sofrem mulitização após 1280ºC. Logo esse concreto exibe um comportamento distinto em função da temperatura de tratamento térmico, pois seu agregado pode se transformar em mulita e sílica. Para esse estudo, amostras prismáticas sinterizadas a 1000ºC e 1450ºC, foram submetidas a ciclos de choque térmico, permanecendo 20 minutos no forno com temperatura de 1000ºC e subseqüente resfriamento em água circulante a 25ºC. Também foram realizadas análises de porosidade aparente, de absorção de água, de massa específica aparente, de difração de raios X e microscopia eletrônica de varredura. Foi verificado que os parâmetros de Hasselman previram que o refratário sinterizado a 1450ºC apresentaria menor resistência ao choque térmico, previsão esta confirmada pelos testes experimentais. Sendo assim é importante avaliar o comportamento do concreto refratário desde a temperatura ambiente até a temperatura de trabalho, a fim de que se conheçam todas as mudanças envolvidas, e que sejam evitados problemas que possam gerar prejuízos na instalação industrial e ao processo pertinente à sua aplicação. / The thermal shock resistance of refractory materials is one of the most important characteristics that determine their performance in many applications, since abrupt and drastic differences in temperature can damage them. A material1s thermal shock resistance may vary according to grain size, the type and intensity of the stress to which it is subjected, the loading rate, and other loading conditions; hence, this resistance is not an intrinsic property of the material. The method currently employed to predict thermal shock behavior is based on the work of Hasselman, whose studies involved the determination of the parameters of thermal shock resistance, R (ºC), thermal shock damage resistance, R\"\" (m), and thermal stress crack stability, Rst (m1/2.ºC). Hasselman\'s equations take into consideration Young\'s modulus, the modulus of rupture, the energy at fracture and the coefficient of thermal expansion of the material under analysis. Resistance to thermal shock damage can be evaluated based on thermal cycles, i.e., successive heating and cooling cycles followed by an analysis of the drop in Young\'s modulus occurring in each cycle. In the present study, samples of a commercial refractory produced by IBAR (Indústrias Brasileiras de Artigos Refratários) were sintered at 1000ºC and 1450ºC for 5 hours to predict and evaluate their thermal shock resistance. These temperatures were chosen because this refractory contains andalusite aggregates that become mullitized at temperatures above 1280ºC. Therefore, this castable displays a particular behavior as a function of the heat treatment temperature, since its aggregates can transform into mullite and silica. In this study, prismatic samples from each sintering temperature were subjected to thermal shock cycles, soaking in the furnace for 20 minutes at a temperature of 1000ºC, and subsequent cooling in circulating water at 25ºC. Apparent porosity, water absorption, apparent density, X-ray diffraction and scanning electron microscopy analyses were also performed. It was found that Hasselman\'s parameters predicted that the refractory sintered at 1450ºC would show lower thermal shock resistance than the refractory sintered at 1000ºC, a prediction that was confirmed by the results of the thermal shock tests. Hence, is important to evaluate the behavior of refractory castables from ambient temperature to working temperature in order to be aware of all the changes that occur, thus avoiding problems leading to losses in industrial installations and in the processes involved in the specific applications of these refractories.

O conhecimento da resistência ao dano por choque térmico de materiais refratários é uma das características mais importantes para determinar seu desempenho em muitas aplicações, pois quando os refratários são submetidos a abruptas e severas variações de temperatura, estes podem sofrer danos. A resistência ao dano por choque térmico de um material pode variar com o tamanho de grão, com o tipo e valor da tensão, com a taxa de carregamento e com outras condições de aplicação da tensão, ou seja, não é uma propriedade intrínseca do material. Os métodos correntemente utilizados para prever o comportamento da resistência ao dano por choque térmico são baseados nos trabalhos de Hasselman, responsável pelo estudo da determinação dos parâmetros de resistência ao choque térmico, R (oC), o parâmetro de resistência ao dano por choque térmico R\'\'\'\' (m), e o parâmetro de estabilidade da trinca sob tensão térmica Rst (m1/2.oC). As equações previstas por ele levam em consideração o módulo de Young, o módulo de ruptura, a energia de fratura e o coeficiente de expansão térmica do material a ser analisado. A resistência ao dano por choque térmico pode ser avaliada por meio de ciclos térmicos, isto é, sucessivos testes de aquecimento e subseqüente resfriamento, com análise da queda do módulo de Young a cada ciclo. Para prever e avaliar a resistência ao dano por choque térmico, amostras de um concreto refratário comercial fornecido pela IBAR (Indústrias Brasileiras de Artigos Refratários), foram sinterizadas a 1000ºC e 1450ºC por cinco horas. Essas temperaturas foram definidas em virtude de o concreto em questão apresentar agregados de andaluzita, que sofrem mulitização após 1280ºC. Logo esse concreto exibe um comportamento distinto em função da temperatura de tratamento térmico, pois seu agregado pode se transformar em mulita e sílica. Para esse estudo, amostras prismáticas sinterizadas a 1000ºC e 1450ºC, foram submetidas a ciclos de choque térmico, permanecendo 20 minutos no forno com temperatura de 1000ºC e subseqüente resfriamento em água circulante a 25ºC. Também foram realizadas análises de porosidade aparente, de absorção de água, de massa específica aparente, de difração de raios X e microscopia eletrônica de varredura. Foi verificado que os parâmetros de Hasselman previram que o refratário sinterizado a 1450ºC apresentaria menor resistência ao choque térmico, previsão esta confirmada pelos testes experimentais. Sendo assim é importante avaliar o comportamento do concreto refratário desde a temperatura ambiente até a temperatura de trabalho, a fim de que se conheçam todas as mudanças envolvidas, e que sejam evitados problemas que possam gerar prejuízos na instalação industrial e ao processo pertinente à sua aplicação. / The thermal shock resistance of refractory materials is one of the most important characteristics that determine their performance in many applications, since abrupt and drastic differences in temperature can damage them. A material1s thermal shock resistance may vary according to grain size, the type and intensity of the stress to which it is subjected, the loading rate, and other loading conditions; hence, this resistance is not an intrinsic property of the material. The method currently employed to predict thermal shock behavior is based on the work of Hasselman, whose studies involved the determination of the parameters of thermal shock resistance, R (ºC), thermal shock damage resistance, R\"\" (m), and thermal stress crack stability, Rst (m1/2.ºC). Hasselman\'s equations take into consideration Young\'s modulus, the modulus of rupture, the energy at fracture and the coefficient of thermal expansion of the material under analysis. Resistance to thermal shock damage can be evaluated based on thermal cycles, i.e., successive heating and cooling cycles followed by an analysis of the drop in Young\'s modulus occurring in each cycle. In the present study, samples of a commercial refractory produced by IBAR (Indústrias Brasileiras de Artigos Refratários) were sintered at 1000ºC and 1450ºC for 5 hours to predict and evaluate their thermal shock resistance. These temperatures were chosen because this refractory contains andalusite aggregates that become mullitized at temperatures above 1280ºC. Therefore, this castable displays a particular behavior as a function of the heat treatment temperature, since its aggregates can transform into mullite and silica. In this study, prismatic samples from each sintering temperature were subjected to thermal shock cycles, soaking in the furnace for 20 minutes at a temperature of 1000ºC, and subsequent cooling in circulating water at 25ºC. Apparent porosity, water absorption, apparent density, X-ray diffraction and scanning electron microscopy analyses were also performed. It was found that Hasselman\'s parameters predicted that the refractory sintered at 1450ºC would show lower thermal shock resistance than the refractory sintered at 1000ºC, a prediction that was confirmed by the results of the thermal shock tests. Hence, is important to evaluate the behavior of refractory castables from ambient temperature to working temperature in order to be aware of all the changes that occur, thus avoiding problems leading to losses in industrial installations and in the processes involved in the specific applications of these refractories.

The finite element method (FEM) has been widely applied to various medical imaging applications over the past two decades. The remarkable progress in high-resolution imaging techniques has allowed FEM to draw great research interests in computing trabecular bone (TB) stiffness from three-dimensional volumetric imaging. However, only a few results are available in literature on applying FEM to multi-row detector CT (MDCT) imaging due to the challenges posed by limited spatial resolution. The research presented here develops new methods to preserve TB structure connectivity and to generate high-quality mesh representation for FEM from relatively low resolution images available at MDCT imaging. Specifically, it introduced a space-variant hysteresis algorithm to threshold local trabecular structure that preserves structure connectivity. Also, mesh generation algorithms was applied to represent TB micro-architecture and mesh quality was compared with that generated by traditional methods. TB stiffness was computed using FEM simulation on micro-CT (µ-CT) and MDCT images of twenty two cadaveric specimens of distal tibia. Actual stiffness of those specimens were experimentally determined by mechanical testing and its correlation with computed stiffness was analyzed. The observed values of linear correlation (r2) between actual bone stiffness and computed stiffness from µ-CT and MDCT imaging were 0.95 and 0.88, respectively. Also, reproducibility of the FEM-based computed bone stiffness was determined from repeat MDCT scans of cadaveric specimens and the observed intra-class correlation coefficient was a high value of 0.98. Experimental results demonstrate the feasibility of application of FEM with high sensitivity and reproducibility on MDCT imaging of TB at distal tibia under in vivo condition.

Nanoindentation is a method of contacting a material whose mechanical properties are unknown with another material whose properties are known. Nanoindentation has the advantage of being able to probe a material’s microstructure while being sensitive enough to detect variations in mechanical properties. However, nanoindentation has some limitations as a testing technique due to the specific formation and structure of some biomaterials. The main objective of this research is to identify any factors that influence the nanoindentation measurement of wood cell walls (a typical biomaterial). The function of the embedding media in describing the properties of wood cells is poorly understood. This research demonstrated that Spurr’s resin, when diffused into wood cell wall during the embedding process, enhanced both the Young’s modulus and hardness of the cell walls. A substitute sample preparation method was developed to avoid this resin penetration into cell wall and was determined to be both effective and easy to perform. The nanoindentation procedure involves the application of a monitor and an analysis of the load-displacement behavior and the response in the material. It can be anticipated that various ways of loading, including the maximum force, the loading time, and others, will cause a variety of mechanical properties. Thus, our second aim was to study the effect of load function on nanoindentation measurement in wood. It was discovered that a fast loading rate contributed to greater contact depth and lower hardness. Increasing the holding time decreased measured values for both Young’s modulus and hardness. However, no significant difference of Young’s modulus and hardness among three loading functions with different unloading rates. The final part of the research was to study the effect of moisture content on the micromechanical properties of wood material. Several nanoindentations were performed on the wood cell wall while varying the moisture content of wood. Results indicated that both the Young’s modulus and hardness decreased significantly with an increase of moisture content. A rheology model was developed to describe the nanoindentation behaviors of wood cell walls at different moisture contents. Five parameters were extracted from Burger’s model, and the relationships among those five parameters were quantified.