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Estudos estruturais da SEPT8 e análise de suas interações com SEPT5 e SEPT7 / Structural studies of SEPT8 and analysis of its interaction with SEPT5 and SEPT7Sinara Teixeira do Brasil Morais 29 May 2014 (has links)
Septinas são proteínas que ligam GTP e interagem entre si formando heterocomplexos, os quais formam filamentos e estruturas de maior nível de organização. Tais filamentos, além de se mostrarem importantes durante a citocinese também podem estar envolvidos em outros processos celulares tais como determinação da polaridade celular e reorganização do citoesqueleto. As septinas, inicialmente descobertas em Saccharomyces cerevisiae, já foram identificadas também em fungos, algas-verdes, mamíferos, porém nunca em plantas. Tipicamente, septinas apresentam três domínios estruturais compostos por um domínio central de ligação ao GTP flanqueado por um N-terminal variável e um C-terminal que pode conter sequências do tipo coiled-coil. Este trabalho teve como objetivo a caracterização do domínio de ligação a GTP da septina 8 humana (SEPT8G) por meio de estudos biofísicos. Nesse sentido, SEPT8G foi eficientemente produzida em E. coli, tendo seu estado dimérico em solução confirmado por cromatografia de exclusão molecular. Diferentemente das outras septinas já reportadas, mesmo dimérica a SEPT8G apresentou-se na forma apo. Ensaios de atividade GTPásica foram realizados, confirmando a incapacidade dessa septina em hidrolisar o GTP. Ainda, análises de estabilidade térmica por Dicroísmo Circular revelaram que a presença do íon magnésio leva à diminuição de sua estabilidade estrutural. Baseando-se em resultados prévios de interação obtidos pela técnica do duplo-híbrido em leveduras, estudos voltados à análise da interação entre as septinas 7 e 8 e, posteriormente, entre as septinas 5, 7 e 8 foram realizados. A co-purificação do complexo formado pelas septinas 7 e 8 mostrou-se dependente da região C-terminal completa de SEPT7 de modo que, quando ausente, a interação mostrou-se expressivamente prejudicada. Já o complexo formado pelas septinas 5/7/8 foi obtido contendo as três proteínas em frações equimolares e solúveis, disponibilizando assim um novo heterocomplexo de septinas para ensaios funcionais e estruturais. / Septins are GTP-binding proteins that interact with each other to form heterocomplexes, which form filaments and higher order structures. These filaments are important for cytokinesis and may be involved in other cellular processes such as the determination of cell polarity and cytoskeleton reorganization. The septins, initially discovered in Saccharomyces cerevisiae, have also been identified in fungi, green algae, mammals but not in plants. Typically, septins have three structural domains comprising a central GTP binding domain flanked by a variable N-terminal and a C-terminal which can contain coiled-coil structures. This work sought to characterize the GTP-binding domain of the human septin 8 (SEPT8G) through biophysical studies. Accordingly, SEPT8G was efficiently produced in E. coli, and its dimeric state in solution was confirmed by size exclusion chromatography. Compared to other septins previously reported, the dimeric SEPT8G presented itself as an apo-protein. GTPase activity assays were performed, confirming the inability of this septin to hidrolyse GTP. Additionally, thermal stability analyses by Circular Dichroism showed that the presence of the magnesium ion leads to a decrease of structural stability. Considering previous results of septins interactions from yeast two-hybrid experiments, we have analyzed the interaction between the septins 7, 8 and subsequently among septins 5, 7 and 8. Co-purification of the complex formed by the septins 7 and 8 showed to be dependent on the complete SEPT7 C-terminal region so that, when absent, the interaction was significantly impaired. The obtained complex formed by septins 5/7/8 contained the three proteins in soluble and equimolar fractions, providing a new septin heterocomplex for functional and structural studies.
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Estudo da eletro-oxidação da mistura H2/CO utilizando eletrocatalisadores à base de Pt/C e Céria dopada com Ítria ou Rutênio para aplicação em células a combustível de membrana polimérica condutora de prótons / Study of electro-oxidation the mix H2/CO using Pt/C Etek and yttria or ruthenium doped ceria electrocatalysts for proton exchange membrane fuel cellLigia Cristina D'Aloia Lulio 06 August 2012 (has links)
As formulações de eletrocatalisadores Pt:(Ce0,9Y0,1O2)+Pt/C Etek, Pt:(Ce0,9Ru0,1O2)+Pt/C Etek, Pt:Ce0,9Y0,1O2/C e Pt:Ce0,9Ru0,1O2+RuO2/C foram preparados pelo método da redução por álcool e caracterizados por difração de raios X (DRX) e microscopia eletrônica de transmissão (MET). A tolerância ao CO foi estudada utilizando o stripping de CO e medidas de curvas de polarização em células unitárias alimentadas com misturas de H2/CO no ânodo e oxigênio no cátodo, na temperatura de 80ºC e pressão absoluta de 2 bar para ânodo e cátodo. Os testes em células a combustível tipo PEMFC mostraram que a oxidação do CO adsorvido a CO2 na superfície da platina ocorre em potenciais menos positivos quando comparado com o catalisador comercial, Pt/C Etek, mostrando tolerância ao CO adsorvido nos eletrocatalisadores de Pt:Ce0,9Y0,1O2/C e Pt:(Ce0,9Ru0,1O2+RuO2)/C. / Pt:(Ce0,9Y0,1O2)+Pt/C Etek, Pt:(Ce0,9Ru0,1O2)+Pt/C Etek, Pt:Ce0,9Y0,1O2/C e Pt:Ce0,9Ru0,1O2+RuO2/C electrocatalysts, were prepared by an alcohol reduction process. The electrocatalysts were characterized by X-Ray Difratometry (XRD) end Transmission Electronic Microscopy (TEM). the CO tolerance was studied using CO stripping and measurements of polarization curves single-cell fed with H2/CO mixture at the anode and oxygen at the cathode, at a temperature of 85ºC and absolute pressure of 2 bar for anode and cathode. The results showed that the oxidation of CO adsorbed to CO2 on the surface of platinum occur at less positive potentials compared to Pt/C Etek showing the tolerance to CO adsorbed on these Pt:Ce0,9Y0,1O2/C e Pt:(Ce0,9Ru0,1O2+RuO2)/C electrocatalysts.
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A crystal engineering study of selected sulfa drugs and trimethoprimElbakush, Rasha Elmheidi January 2014 (has links)
Magister Pharmaceuticae - MPharm / The objective was to prepare new solid phases, i.e. co-crystal forms, of two sulfa antibiotic drugs (sulfamethoxazole and sulfasalazine) with trimethoprim and fourteen potential co-formers with GRAS status. Trimethoprim was chosen for its synergistic effects with both sulfa drugs and the other co-formers were selected in an attempt to improve the physicochemical properties of the antibiotics. A variety of co-crystallization techniques, including solvent assisted grinding, slow evaporation, slurry method and solidification of the melt were used to obtain these results. From these methods, three new solid phases were successfully isolated for the sulfamethoxazole antibiotic, viz. sulfamethoxazole-benzoic anhydride (SMZ-BAN) co-crystal by the slurry method, amorphous sulfamethoxazole-trimethoprim (SMZ-TMP) form by solidification of the melt and amorphous sulfamethoxazole-oxalic acid (SMZ-OA) by slow evaporation. For the sulfasalazine antibiotic, co-crystallization experimentation produced, sulfasalazine-trimethoprim salt (SSZ-TMPs) by slow evaporation, sulfasalazine-trimethoprim co-crystal (SSZ-TMP) by solvent assisted grinding and sulfasalazine-nicotinamide co-crystal (SSZ-NC) by solidification of the melt. Of these six compounds subjected to single crystal X-ray analysis, only one of their structures was elucidated i.e. the salt, SSZ-TMPs. Different techniques that were used to assess the thermal behaviour of the products included hot stage microscopy, differential scanning calorimetry and thermogravimetric analysis. FTIR provided information on the purity of the compounds and the suggested host-guest interaction sites. X-ray powder diffraction supported the determination of the new phase comparative to the parent compounds. Finally dissolution testing was carried out for successful candidates
with encouraging recommendations for future work.
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Hybridization of 4d Metal Nanoparticles with Metal-Organic Framework and the Investigation of the Catalytic Property / 4d遷移金属ナノ粒子と金属有機構造体の複合化による触媒活性変化の研究Aoyama, Yoshimasa 27 July 2020 (has links)
京都大学 / 0048 / 新制・課程博士 / 博士(理学) / 甲第22684号 / 理博第4625号 / 新制||理||1665(附属図書館) / 京都大学大学院理学研究科化学専攻 / (主査)教授 北川 宏, 教授 吉村 一良, 教授 有賀 哲也 / 学位規則第4条第1項該当 / Doctor of Science / Kyoto University / DGAM
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Valorisation énergétique de CO₂ via la méthanation par voie catalytique / Energy recovery of CO₂ through methanation process by catalyticallyElia, Nathalie 22 March 2019 (has links)
Cette étude concerne la valorisation du dioxyde de carbone par le procédé de méthanation. Elle vise à mettre au point des catalyseurs efficaces pour cette réaction. L'espèce active est le nickel métallique. Différents supports ont été étudiés tels que SiO₂, Al₂O₃, MgO, Y₂O₃ et CeO₂. Ces catalyseurs ont été préparés par la méthode d'imprégnation à sec. Dans un premier temps, les différents catalyseurs ont été caractérisés par différentes techniques physico-chimiques dont la Diffraction des Rayons X (DRX), la Réduction en Température Programmée (RTP-H₂), la Désorption en Température Programmée (DTP-CO₂), l'Adsorption d'Azote (méthode BET) et la Chimisorption d'Hydrogène. Dans un deuxième temps, les différents catalyseurs ainsi préparés ont été testés dans la réaction de méthanation du CO₂. Le catalyseur Ni/CeO₂ présente les meilleures performances catalytiques, parmi les systèmes étudiés. L'ajout du ruthénium améliore l'activité catalytique et la stabilité des catalyseurs. Le catalyseur Ru(0,5%)-Ni(5%)/CeO₂ est le plus performant, il présente une bonne activité catalytique et une bonne stabilité même pour une pression de 10 bar. Ceci le rend plus avantageux pour une application industrielle. / This study concerns the valorization of carbon dioxide by the methanation process. It aims to develop effectiv catalysts for this reaction. The active species is metallic nickel. Different supports have been studied such as SiO₂, Al₂O₃, MgO, Y₂O₃ and CeO₂. These catalysts were prepared by the dry impregnation method. Initially, the different catalysts were characterized by different physicochemical techniques including X-ray Diffraction (XRD), Temperature Programmed Reduction (TPR-H₂), Temperature Programmed Desorption (TPD-CO₂), nitrogen adsorption (BET method) and hydrogen chemisorption. In a second step, the various catalysts thus prepared were tested in the CO₂ methanation reaction. The Ni/CeO₂ catalysts has the best catalytic performance, among the systems studied. The addition of ruthenium improves the catalytic activity and the stability of the catalysts. The catalyst Ru(0.5%)-Ni(5%)/CeO₂ is the most efficient, it has good catalytic activity and good stability even as a pressure of 10 bar. This makes it advantageous for an industrial application.
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Teaching During the COVID-19 Pandemic: Exploring Co-Planning, Co-Teaching, and Preservice Teachers' Self-EfficacySmith, Mary Beth Zavodnik 10 August 2022 (has links)
No description available.
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Biochemical Characterization Of The Nifb Enzyme And Nifb-cofactorGevorkyan, Jirair 01 January 2013 (has links)
The Mo-nitrogenase complex is composed of two components, Fe-protein and MoFe-protein. This complex is able to catalyze the reduction of N2 through the MgATP dependent transfer of electrons from the Fe-protein Fe4S4 cluster to the MoFe-protein P-cluster and, subsequently, to the iron-molybdenum cofactor (FeMoco). FeMo-co is a Fe7S9MoC-(R)-homocitrate cluster and has two biosynthetic precursors, NifB-co and L-cluster, of unknown structure and composition. The biosynthesis of FeMo-co is an enigmatic process that minimally requires NifB, NifEN, Fe-protein, MoO4 2- , (R)-homocitrate and S-adenolsylmethionine. A means to isolate the NifB enzyme for characterization has been developed through use of a GST-fusion tag. Double recombination of A. vinelandii strains with a constructed vector has yielded strains capable of nif promoter regulated expression of GST-NifB. Extracts of strains containing GST-NifB were shown to activate the Monitrogenase complex in biochemical complementation assays. Mass spectroscopy was then used to verify successful isolation of GST-NifB by GSH-Sepharose affinity purification. The number of NifB-co ligand binding sites and ligand types were examined by EXAFS analysis of samples containing selenol and thiol ligands. A Fe6S9C model for NifB-co was optimized to best fit the EXAFS data, where a 2-fold discrepancy in binding sites implied by thiol or selenol only ligand samples suggests Fe-(μ2S)-Fe binding in the absence of Se. Samples containing heterogeneous ligand types indicated that NifX bound NifB-co ligates to four cysteine residues and one molecule of DTT.
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Tvålärarsystem som pedagogisk praktik : En kvalitativ studie om hur tio lärarpar beskriver hur tvålärarsystemet påverkar deras undervisning och möjligheten att möta elevernas behovBjörk, Karolina January 2023 (has links)
Tvålärarsystem är ett arbetssätt som blir vanligare i svenska skolan. Det går ut på att två klass–eller ämneslärare delar på ansvaret för eleverna och dess tillhörande planering, undervisning och bedömning. Syftet med den här studien är att bidra med kunskap om hur tvålärarsystemet kan organiseras och genomföras i svensk kontext och hur arbetssättet ur ett lärarperspektiv påverkar undervisningen och möjligheter att möta elevernas behov. Det för att fylla den kunskapslucka som saknaden av svensk forskning kring tvålärarsystemet ger. I kapitlet tidigare forskning presenteras internationell forskning om tvålärarsystemet som kallas co-teaching. Då det saknas forskning om tvålärarsystemet i ett svenskt perspektiv kommer därför även beprövad dokumenterad erfarenhet om tvålärarsystem i Sverige att presenteras. Utifrån den tidigare forskningen framkommer två bärande begrepp ”kollegialt samarbete i klassrummet” samt ”gruppering”, dessa kommer ligga som ett teoretiskt raster över studien. I metodkapitlet beskrivs studiens tillvägagångssätt, beskrivning av informanterna, analysprocessen, etiska övervägande och en metoddiskussion. I resultatkapitlet presenteras informanternas beskrivning av undervisning och vilka konsekvenser det ger upphov till. Fokus ligger på vad den läraren som inte håller i undervisningen gör. I resultatet beskrivs även hur informanterna upplever att arbetssättet påverkar eleverna samt lärarnas möjlighet att möta elevernas behov. I diskussionsavsnittet förs resonemang kring resultatet i förhållande till den tidigare forskningen och de beprövade dokumenterade erfarenheter som presenterats i studien. Slutsatser utifrån studiens resultat är att lärarna är positivt inställda och upplever det lättare att möta elevernas behov, göra anpassningar och skapa en roligare och bättre undervisning. Det framkom att respondenterna saknar bevis för att tvålärarsystemet ökar elevernas måluppfyllelse men att alla har en känsla av att måluppfyllelsen ökar eftersom de känner att de kan möta fler elevers behov. En viktigt förutsättning för arbetssättet är att lärarnas samarbete fungerar. Ytterligare en viktig slutsats är att det behövs forskning om tvålärarsystemet ur ett svenskt perspektiv.
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HARDWARE/SOFTWARE CO-DEBUGGING FOR RECONFIGURABLE COMPUTING APPLICATIONSTIWARI, ANURAG 30 January 2002 (has links)
No description available.
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Structural and transport properties of Co/Re and Co/Ti multilayer filmsHuai, Yiming January 1992 (has links)
Thèse numérisée par la Direction des bibliothèques de l'Université de Montréal.
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