published_or_final_version / Business Administration / Master / Master of Business Administration

published_or_final_version / abstract / Architecture / Master / Master of Science in Interdisciplinary Design and Management

published_or_final_version / Housing Management / Master / Master of Housing Management

Different strategies can be used to repair, rehabilitate and strengthen existing structures. Techniques based on Fiber Reinforced Polymer (FRP) materials appear to be innovative alternatives to traditional solutions because of their high tensile strength, light, weight, and ease of installation. One of the most common and useful FRPs is Carbon Fiber Reinforced Polymer (CFRP) used in sheets and anchors attached on the concrete surface to strengthen the section through addition of tensile capacity. The purpose of this study was develop a technique for assesses the strength of anchors for quality control purpose. However, to transfer tensile capacity to a concrete surface, the sheets are bonded to the surface with epoxy adhesive. As tension increase, CFRP sheets lose adherence of the epoxy from the concrete surface and finally debond. To avoid this failure, CFRP anchors are applied in addition at the epoxy. The CFRP anchors allow the CFRP sheets to utilize their full tensile capacity and maximize the material efficiency of the CFRP retrofit. The number and size of anchors play a critical role. However the capacity of CFRP anchors has not been investigated extendedly. A methodology for assessing the quality of CFRP anchors was developed using plain concrete beams and reinforced externally with CFRP sheets attached with epoxy and CFRP anchors. Applying load to the beam, allowed the development a tensile force in the CFRP sheets and a shear force on the CFRP anchors. The shear forces in the CFRP anchors were defined by the load applied to the beam and compared with forces based on measured stress in CFRP sheets. / text

Run-of-mine (ROM) coal sampling is one of the most important factors in determining the disposition of ROM coal for an overall emission control strategy. Determination of the amount of sample, or still better, the frequency of ROM coal sampling is thus essential to the analysis of overall emission control strategies. A simulation model of a portion of the Upper Freeport coal seam in western Pennsylvania was developed employing conditional simulation. On the simulated deposit, different mining methods were simulated to generate ROM coal data. ROM coal data was statistically analyzed to determine the sampling frequency. Two schemes were suggested: (1) the use of geostatistical techniques if there is spatial correlation in ROM coal quality, and (2) the use of classical statistics if the spatial correlation in ROM coal quality is not present. Conditions under which spatial correlation in ROM coal quality can be expected are also examined. To link the ROM coal and coals from other sources to coal stockpiles and subsequently to solve coal blending problems, where varying qualities of stockpiled coals are normally used, an interactive computer program was developed. Simple file-handling, for stockpiling problems, and multi-objective goal programming technique, for blending problems, provided their solutions. The computer program was made suitable for use on both minicomputer and microcomputer. Menu-driven and interactive capabilities give this program a high level of flexibility that is needed to analyze and solve stockpiling and blending problems at the power plant.

In eukaryotes, mRNA decay pathways are important for cellular response to various physiological conditions and also function in co-translational quality control systems that target translationally aberrant mRNAs for degradation. My work on identification and characterization of novel components and pathways of mRNA degradation and quality control in Saccharomyces cerevisiae is summarized below.I have identified Edc3p as a novel protein important for mRNA decay. Deletion of Edc3p leads to a defect in mRNA decay in strains deficient in decapping enzymes and, in combination with a block to the 3' to 5' decay pathway, causes exaggerated growth defects and synthetic lethality. An Edc3p-GFP fusion protein localizes in processing bodies, which are specialized cytoplasmic foci containing decapping proteins. Together, these observations indicate that Edc3p directly interacts with the decapping complex to stimulate the mRNA decapping rate.Quality control during mRNA translation is critical for regulation of gene expression. My work shows that yeast mRNAs with defects in translation elongation, due to strong translational pauses, are recognized and targeted for degradation via an endonucleolytic cleavage in a novel process referred to as No-Go Decay (NGD). The cellular mRNA decay machinery degrades the 5' and 3' cleavage products produced by NGD. NGD is translation-dependent, occurs in a range of mRNAs and can be induced by a variety of elongation pauses. These results indicate NGD may occur at some rate in response to any stalled ribosome.I also show that two highly conserved proteins, Dom34p and Hbs1p, homologous to the eukaryotic release factors eRF1 and eRF3 respectively, are required for NGD. Further characterization of the No-Go decay pathway indicates that Dom34p function during NGD is conserved across species. Identification of RPS30, a small ribosomal protein as a trans-acting factor during NGD suggests that the ribosome may have a novel role during NGD. Other experiments indicate that the No-Go decay pathway may cross talk with the unfolded protein response pathway. The identification of No-Go decay as a novel quality control pathway during translation elongation supports the existence of a global cellular mechanism for maintenance of translational quality control.

Image sizes have increased exponentially in recent years. The resulting high-resolution images are typically encoded in a lossy fashion to achieve high compression ratios. Lossy compression can be categorized into visually lossless and visually lossy compression depending on the visibility of compression artifacts. This dissertation proposes visually lossless coding methods as well as a visually lossy coding method with perceptual quality control. All resulting codestreams are JPEG2000 Part-I compliant.Visually lossless coding is increasingly considered as an alternative to numerically lossless coding. In order to hide compression artifacts caused by quantization, visibility thresholds (VTs) are measured and used for quantization of subbands in JPEG2000. In this work, VTs are experimentally determined from statistically modeled quantization distortion, which is based on the distribution of wavelet coefficients and the dead-zone quantizer of JPEG2000. The resulting VTs are adjusted for locally changing background through a visual masking model, and then used to determine the minimum number of coding passes to be included in a codestream for visually lossless quality under desired viewing conditions. The proposed coding scheme successfully yields visually lossless images at competitive bitrates compared to those of numerically lossless coding and visually lossless algorithms in the literature.This dissertation also investigates changes in VTs as a function of display resolution and proposes a method which effectively incorporates multiple VTs for various display resolutions into the JPEG2000 framework. The proposed coding method allows for visually lossless decoding at resolutions natively supported by the wavelet transform as well as arbitrary intermediate resolutions, using only a fraction of the full-resolution codestream. When images are browsed remotely, this method can significantly reduce bandwidth usage.Contrary to images encoded in the visually lossless manner, highly compressed images inevitably have visible compression artifacts. To minimize these artifacts, many compression algorithms exploit the varying sensitivity of the human visual system (HVS) to different frequencies, which is typically obtained at the near-threshold level where distortion is just noticeable. However, it is unclear that the same frequency sensitivity applies at the supra-threshold level where distortion is highly visible. In this dissertation, the sensitivity of the HVS for several supra-threshold distortion levels is measured based on the JPEG2000 quantization distortion model. Then, a low-complexity JPEG2000 encoder using the measured sensitivity is described. The proposed visually lossy encoder significantly reduces encoding time while maintaining superior visual quality compared with conventional JPEG2000 encoders.

No description available.

Mitochondria are dynamic; they alter their shape through fission, fusion and budding of vesicles. Mitochondrial vesicles serve as a quality control mechanism enabling these organelles to rid themselves of damaged lipids and proteins. Dysregulation in mitochondrial dynamics and quality control have been linked to Parkinson’s Disease, making the identification of molecules requisite for these processes a priority. We identified the endocytic protein, Sorting nexin 9 (Snx9) through a genome wide siRNA screen for genes which substantially alter mitochondrial morphology and therefore are important for its maintenance. In this work, the role of Snx9 in mitochondrial morphology is examined. Ultrastructural imaging of mitochondria within cells silenced for Snx9 revealed unbudded vesicles along a hyperfused mitochondrial reticulum suggesting a role for Snx9 in the release of these vesicles. The vesicular profiles contained concentric membranous whorls enriched for neutral lipids. Localization studies suggest the Parkinson’s disease genes, Parkin and Vps35 localize to the unbudded profiles.

Activation of the stress-responsive transcription factor NRF2 is the major line of defense to combat oxidative or electrophilic insults. Under basal conditions, NRF2 is continuously ubiquitylated by the KEAP1-CUL3-RBX1 E3 ubiquitin ligase complex and is targeted to the proteasome for degradation ( the canonical mechanism). However, the path from the CUL3 complex to ultimate proteasomal degradation was previously unknown. p97 is a ubiquitin-targeted ATP-dependent segregase that extracts ubiquitylated client proteins from membranes, protein complexes, or chromatin and has an essential role in autophagy and the ubiquitin proteasome system ( UPS). In this study, we show that p97 negatively regulates NRF2 through the canonical pathway by extracting ubiquitylated NRF2 from the KEAP1-CUL3 E3 complex, with the aid of the heterodimeric cofactor UFD1/NPL4 and the UBA-UBX containing protein UBXN7, for efficient proteasomal degradation. Given the role of NRF2 in chemoresistance and the surging interest in p97 inhibitors to treat cancers, our results indicate that dual p97/NRF2 inhibitors may offer a more potent and long-term avenue of p97-targeted treatment.