The Northern Isles are strongly influenced by changes in the North Atlantic Ocean atmosphere system and, as they project northwards from the British Isles, provide an ideal geographical opportunity to study changing climatic gradients during the last glacial/interglacial transition along with the detection of regime shifts. Three proxies, diatoms, pollen, and micro-XRF sediment chemistry, have been employed to explore the nature and timing of environmental changes within the water columns and the wider catchments of Loch of Sabiston, Orkney, and Loch of Clumlie and Loch of Grimsetter, Shetland to better understand the nature and timing of environmental change within and among the island groups. The records are constrained by radiocarbon dating, supported by tephrochronology, and the Greenland ice core chronology to enable the comparison of the records produced by this study with previous research in the North Atlantic region. The diatom and lithological results from Loch of Sabiston suggest early deglaciation at c. 23,000 cal BP followed by gradual warming (GI-1e) punctuated by the cooling events coeval with GS-1 and GI-1b. However, the pollen record reflects a lagged response in the development from colonising cold tolerant vegetation to more temperate shrub and woodland communities. The Oracadian signal is dominated by the switching on and off of the accumulation of marl which serves as a supporting indicator of warmer conditions. The Shetland landscape appears to have been deglaciated later at c. 16,400 cal BP, but also has clear representation of GI-1e and the cooling events of GI-1b and GS-1. Both the Shetland and Orkney records record the dramatic cooling of the Younger Dryas but also suggest a two stage change from colder and drier to colder and wetter conditions before the onset of the Holocene. Shetland appears to have experienced less extreme climatic changes in comparison to Orkney despite being in the same present phytogeographical region. This is likely due to the former persisting in the arctic domain and the latter being closer to the latitudinal shifts in the warmer ocean circulation of the North Atlantic during the LGIT. Comparison of the three proxies demonstrates that they may differ by several hundred years in their response to dramatic climatic changes and, therefore, highlights the strength of multi-proxy approaches to reconstructing Quaternary environments. Combining proxies such as diatom and μ-XRF scanning techniques will provide a greater understanding of the processes occurring during environmental change in this region.

<p>Flight Control Systems are often designed in linearization points over a flight envelope and it must be proven to clearance authorities that the system works for different parameter variations and failures all over this envelope. </p><p>In this thesis µ-analysis is tried as a complement for linear analysis in the frequency plane. Using this method stability can be guaranteed for all static parameter combinations modelled and linear criteria such as phase and gain margins and most unstable eigenvalue can be included in the analysis. A way of including bounds on the parameter variations using parameter dependent Lyapunov functions is also tried. </p><p>To perform µ-analysis the system must be described as a Linear Fractional Transformation (LFT). This is a way of reformulating a parameter dependent system description as an interconnection of a nominal linear time invariant system and a structured parameter block. </p><p>A linear and a rational approximation of the system are used to make LFTs. These methods are compared. Four algorithms for calculation of the upper and lower bounds of µ are evaluated. The methods are tried on VEGAS, a SAAB research aircraft model. </p><p>µ-analysis works quite well for linear clearance. The rational approximation LFT gives best results and can be cleared for the criteria mentioned above. A combination of the algorithms is used for best results. When the Lyapunov based method is used the size of the problem grows quite fast and, due to numerical problems, stability can only be guaranteed for a reduced model.</p>

Flight Control Systems are often designed in linearization points over a flight envelope and it must be proven to clearance authorities that the system works for different parameter variations and failures all over this envelope. In this thesis µ-analysis is tried as a complement for linear analysis in the frequency plane. Using this method stability can be guaranteed for all static parameter combinations modelled and linear criteria such as phase and gain margins and most unstable eigenvalue can be included in the analysis. A way of including bounds on the parameter variations using parameter dependent Lyapunov functions is also tried. To perform µ-analysis the system must be described as a Linear Fractional Transformation (LFT). This is a way of reformulating a parameter dependent system description as an interconnection of a nominal linear time invariant system and a structured parameter block. A linear and a rational approximation of the system are used to make LFTs. These methods are compared. Four algorithms for calculation of the upper and lower bounds of µ are evaluated. The methods are tried on VEGAS, a SAAB research aircraft model. µ-analysis works quite well for linear clearance. The rational approximation LFT gives best results and can be cleared for the criteria mentioned above. A combination of the algorithms is used for best results. When the Lyapunov based method is used the size of the problem grows quite fast and, due to numerical problems, stability can only be guaranteed for a reduced model.

Ce travail propose une méthodologie d'analyse et de synthèse de lois de commande robustes pour les systèmes dynamiques plats perturbés. Pour une famille de modèles perturbés, le suivi de trajectoire nominale déterminée à l'aide du concept de platitude, est garanti par un régulateur linéaire déterminé par optimisation de critères . Cette synthèse et/ou analyse est basée dans un premier temps sur une famille de modèle LTI modélisant le comportement dynamique du procédé au voisinage de la trajectoire de référence. En vue d'une réduction du conservatisme une nouvelle méthodologie est ensuite proposée pour obtenir une modélisation LPV de la dynamique de l'écart de trajectoire. Cette démarche est appliquée à la synthèse d'une loi de commande robuste pour un procédé multivariable et non linéaire, puis à l'analyse d'une loi de commande de vol dans le cadre du groupe de Recherche Européen GARTEUR AG11.

Cette thèse porte sur une des préoccupations majeures dans la conception des systèmes automatisés : la tolérance aux défauts. L'interaction entre les tâches de surveillance et de reconfiguration de la loi de commande est examinée et un nouveau schéma coopératif est proposé pour la gestion globale des compromis. Cette architecture présente l'avantage de préserver entièrement les performances nominales (situation non défaillante) car la boucle de commande nominale est conservée au sein du schéma coopératif. Une procédure de synthèse basée sur la mise en œuvre et l'évaluation des indicateurs de performance des modules de diagnostic et de commande, est proposée. Les résultats méthodologiques sont appliqués au modèle d'un avion gros porteur : le Boeing 747-100/200. Ce Benchmark a été établi par le groupe européen GARTEUR (AG16, Fault Tolerant Control) afin de comparer les méthodologies actives de la commande tolérante aux fautes avec les lois classiques de pilotage.

In this work, we investigated the behavior of the -opioid receptor (MOR) and the transient receptor potential vanilloid 1 (TRPV1) ion channel in the plasma membrane and their mutual communication. Both these receptors are implicated in pain perception and analgesia. We observed that the lateral mobility of MOR was strongly affected by different biased opioid agonists. DAMGO and endomorphin-2 display opposite bias towards MOR. According to our results, they also have the opposite effects on the mobility of MOR. Morphine induced only small changes in the mobility of MOR. Moreover, cholesterol depletion and blockage of G protein signaling by pertussis toxin (PTX) affected the ability of different MOR agonists to alter MOR mobility in a unique manner. The effects of DAMGO and endomorphin-2 were compromised under these conditions. On the other hand, we observed increased movement of MOR after the addition of morphine. PTX alone did not affect receptor movement, but it completely disrupted the effect of cholesterol depletion on morphine induced changes the mobility of MOR. Next we studied the mobility of TRPV1. The TRPV1 agonist capsaicin changed the lateral mobility of TRPV1. Surprisingly, after adding the MOR antagonist naloxone, the apparent diffusion coefficient of TRPV1 but to a lower extent than...

In this thesis, micro computed tomography (μ−CT) scans of a bio-glass scaffold produced by the robocasting technique was used to create finite element method (FEM) models with the purpose of determining its mechanical strength. Prior to this, a Matlab script was used to create several simplified geometries of the scaffold in an effort to determine the importance of scaffold design parameters (such as the fiber compenetration between two adjacent printing planes) on the strength of the scaffold. Furthermore, to assess the influence of micro-structural defects such as voids and micro-cracks that are intrinsic to the robocasting manufacturing process, the total number of voids and their respective volume was calculated using the μ-CT scan imagery and fitted to a statistical distribution. The distribution of voids was then used to create several scaffold models in Matlab with either spherical or ellipsoidal voids present. In the final two models, one scaled-down and one scaled-up FEM based on μ-CT scans were investigated.To model the crack initiation, propagation and final failure, the phase-field method was used. The method was implemented by the use of a publicly available Fortran user subroutine and was edited to account for asymmetric tension/compression energy degradation. The resulting strength of the produced models have been presented as non-dimensional values. The finite element analysis (FEA) of the Matlab produced scaffolds showed that the fiber shifting between two adjacent layers, porosity, and voids of ellipsoidal shape that were perpendicular to the loading direction had the highest effect on the strength of the scaffold. The resulting normalized strength values obtained from the μ-CT models was partially validated through a comparison with the literature available.The different failure modes and overall architectural arrangement of cracks also showed promising results. / I den här uppsatsen så användes mikrotomografi (μ-CT) skanning av en bio-glas stödstruktur tillverkad av robocasting tekniken för att skapa finita element modeller med syftet att bestämma dess mekaniska styrka. Innan detta så användes ett Matlab-skript för att skapa flera förenklade geometrier av stödstrukturen i ett försök att fastställa betydelsen av viktiga designparametrar (som t.ex fiberpenetrering mellan två intilliggande plan) på stödstrukturens styrka. Vidare, för att bedöma påverkan av mikrostrukturella defekter som tomrum och mikrosprickor som är naturligt förekommande i robocasting-tillverkningsprocessen så uppmättes det totala antal hålrum och deras respektive volym med hjälp av μ-CT-skannade bilder. Denna data blev anpassad till en statistisk fördelning. Fördelningen av tomrum och mikcrosprickor användes sedan för att skapa flera modeller av stödstrukturerna i Matlab med antingen sfäriska eller ellipsoida hålrum närvarande. I de sista två modellerna undersöktes en en nedskalad och en uppskalad finita elementmodell baserad på μ-CT-skanning.För att modellera sprickinitiering, sprickpropagering och slutligen brott användes fasfältsmetoden. Fasfältsmetoden implementerades med hjälp av en för allmänheten tillgänglig Fortran användarrutin som redigerades för att ta hänsyn till en asymmetrisk energidegradering i drag-och tryck. Den resulterande styrkan hos alla modeller har presenterats som icke-dimensionella värden. Finita elementanalysen av Matlab modellerna visade att fiberskiftningen mellan två intilliggande plan, porositet och hålrum med ellipsoid form som var vinkelräta mot belastningsriktningen hade störst effekt på stödstrukturens styrka. De resulterande normaliserade styrkevärdena erhållna från μ-CTmodeller validerades delvis genom en jämförelse med tillgänglig litteratur. Dom olika felmoderna och övergripande strukturella fördelningen av sprickor visade också lovande resultat.

-5- ABSTRACT A huge effort has been put in determining the mechanism of the development of tolerance and dependence in context of clinical use of morphine for treatment of severe pain. Understanding of this mechanism would help to design new and more efficient pharmaceuticals. This diploma paper discus the opiate receptors with a special focus on long-term effect of chronic morphine treatment, which was determined using a radioligand binding assays with a non-selective antagonist [3 H]Diprenorphine. One of the goals of this work was to create and optimise a method for preparation of pure plasma membranes from rat cortex using percoll gradient. There were five groups, which differed in the length of morphine treatment: ten days (M-10), twenty-eight days (M-28), ten days with seven days of regression (RM-10 twenty-eight days with seven days of regression (RM-28) and a control group (K). The loss of total opioid receptor number was noticeable after ten days and grew slightly during continuous morphine treatment and kept lowering in the period of regression. The total loss was approximately 30% of the control binding. The equilibrium dissociation constant (Kd), thus the affinity of [3 H]Diprenorphine wasn't significantly different among the groups. Morphine acts through µ-opioid receptor, that's why there was a...

Le centre de recherche ArcelorMittal de Montataire développent de nouvelles solutions acier innovantes associant métal et polymère. Pour les ailes de voiture, le composite retenu est un matériau multicouche composé d’une lame d’acier sur laquelle est déposé un film mince de polypropylène choc chargé avec des particules minérales par l’intermédiaire d’une fine couche de polypropylène fonctionnalisé par le greffage d’anhydride maléique. Afin de prévoir et de connaitre le comportement de la partie organique du matériau lors de sa mise en forme par emboutissage et à posteriori de prédire l’état de ses propriétés mécaniques lors de son utilisation, la détermination des lois de comportement mécanique vrai et intrinsèque sur le modèle de la loi G’sell et Jonas est nécessaire. Ces lois sont définies selon trois chemins de déformation : la traction uniaxiale, le cisaillement simple et la traction plane. Les micromécanismes de déformation de la microstructure semi-cristalline des différentes formulations des matériaux selon leur mode de sollicitation mécanique ont été étudiés. Les résultats obtenus Post Mortem et In Situ ont permis la description qualitative et quantitative des évolutions des principales modifications microstructurales. Ces dernières diffèrent avec l’ajout de charges minérales. Deux nouvelles méthodes, la Tomographie X et la spectroscopie Raman permettent la détermination de la déformation volumique dans le cas de matériau de géométrie fine (300 µm). Le retrait lors d’un cycle thermique est étudié. Les influences du chauffage, de la formulation et de la microstructure (orientation des chaînes macromoléculaires et endommagement volumique) sont décrites / The ArcelorMittal research centre of Montataire elaborates innovative steel / polymer products. In the case of automotive fenders, the composite is a multilayered material. A thin impact polypropylene film is laminated on steel using a thin layer of a functionalized polypropylene. Mineral particles are added to improve stiffness. In order to predict and understand the behaviour of the organic layer all along its production process and finally to be able to characterize the state of its mechanical properties in use, the determination of the true and intrinsic mechanical behaviour laws according to the G’sell and Jonas model is necessary. These laws are obtained for three different mechanical paths: uniaxial tensile, simple shear and plane tensile. The deformation micromechanisms of the impact polypropylene semi-crystalline microstructure which depend on the materials formulations and on the mechanical path used are studied. Post Mortem and In Situ results give qualitative and quantitative description of the main microstructural modifications. Two new methods, X Tomography and Raman spectroscopy allow the quantification of the volume deformation which is developed during tensile tests. They are mainly available for very thin samples. X radiography and VideoTraction™ are not suitable anymore for this kind of geometry. Finally, the thermo-mechanical phenomenon of shrinkage which occurs during thermal treatment above the material melting point is analysed. Influences of the heating conditions, of the material formulations and of the material microstructure are described. Special overviews are done on the macromolecular chains orientation and on the volume damage influences

Analysis of compounds present in complex matrices is always a challenge, which can be partly overcome by applying various sample preparation techniques prior to detection. Ideally, the extraction techniques should be as selective as possible, to minimize the concentration of interfering substances. In addition, results can be improved by efficient chromatographic separation of the sample components. The elimination of interfering substances is especially important when utilizing mass spectrometry (MS) as a detection technique since they influence the ionization yields. It is also important to optimize ionization methods in order to minimize detection limits. In the work this thesis is based upon, selective solid phase extraction (SPE) materials, a restricted access material (RAM) and graphitized carbon black (GCB) were employed for clean up and/or pre-concentration of analytes in plasma, urine and agricultural drainage water prior to liquid chromatography/mass spectrometry (LC/MS). Two SPE formats, in which GCB was incorporated in µ-traps and disks, were developed for cleaning up small and large volume samples, respectively. In addition, techniques based on use of sub-2 µm C18 particles at elevated temperatures and a linear ion trap (LIT) mass spectrometer were developed to improve the efficiency of LC separation and sensitivity of detection of 6-formylindolo[3,2-b]carbazole (FICZ) metabolites in human urine. It was also found that GCB can serve not only as a SPE sorbent, but also as a valuable surface for surface-assisted laser desorption ionization (SALDI) of small molecules. The dual functionality of GCB was utilized in a combined screening-identification/quantification procedure for fast elimination of negative samples. This may be particularly useful when processing large numbers of samples. SALDI analyses of small molecules was further investigated and improved by employing two kinds of new surfaces: oxidized GCB nanoparticles and silicon nitride.