Células mesenquimais indiferenciadas humanas foram obtidas por digestão enzimática e centrifugação do produto de lipoaspiração, expandidas in vitro, e implantadas no tecido subcutâneo de camundongos atímicos. No grupo I, cada animal recebeu o implante de uma membrana de 0,25cm2 de ácido glicólico e carbonato de trimetileno semeada com 1 x 106 destas células .No grupo II, cada um recebeu a injeção de 0,2ml de gel de ácido hialurônico reticulado contendo o mesmo número destas células. Com três semanas de implante, células humanas e vasos foram identificados nos dois carreadores. Entretanto, com oito semanas, somente no gel de ácido hialurônico as células humanas e os vasos estavam presentes / Human undifferentiated mesenchymal cells were obtained by enzymatic digestion and centrifugation of the product of liposuction. These cells were expanded, in vitro, and implanted subcutaneously in athymic mice. In group I, each animal received the implant of a 0,25cm2 membrane of glycolic acid and trimethylene carbonate, seeded with 1 x 106 of these cells. In group II, each one received 0,2 ml of cross-linked hyaluronic acid gel containing the same amount of these cells. With three weeks of implantation, human cells and vessels were identified in both carriers. However, with eight weeks of implantation, only in hyaluronic acid gel human cells and vessels were present

O objetivo deste trabalho foi comparar aspectos clínicos de pacientes portadores de fibromialgia cujo início dos sintomas estivessem relacionados a eventos traumáticos e pacientes idiopáticos. Foram avaliados 135 pacientes por meio de um questionário estruturado sobre dados demográficos e situação de produtividade laboral, caracterização da dor e presença de queixas não relacionadas ao aparelho locomotor. Foi realizada contagem e dolorimetria de pressão dos pontos dolorosos e avaliação da dor segundo a escala visual analógica. O grupo de pacientes traumáticos conteve 48 pessoas apresentou maior período médio de escolaridade (8,1 &#177; 4,1 x 5,3 &#177; 2,9; p<0,001), maior contagem de pontos dolorosos (16,1 &#177; 2,8 x 15,0 &#177; 3,2; p = 0,047), menor período desde a generalização da dor (5,6 &#117; 6,9 x 4,1 &#177; 2,6 anos; p = 0,002) e maiores prevalências de dificuldade de concentração (83,0% x 65,1%; p = 0,048) e cólicas intestinais (45,8% x 26,4%; p = 0,036). Houve associação entre o trauma e a improdutividade econômica. Os demais aspetos clínicos não apresentaram diferença estatisticamente significante. Conclui-se que a etiologia traumática impõe poucas diferenças clínicas aos pacientes portadores de fibromialgia e não explicam o grau de incapacidade laboral que se observa. / The aim of this work was to compare clinical aspects of fibromyalgia patients whose onset was related to traumatic events to idiopathic patients. One hundred and thirty fice consecutive patients were interviewed with a structured questionnaire about demographic aspects, work disability, pain characterization and the presence of symptoms not related to the locomotor system. Tender point count and dolorimetry were performed, as well as pain evaluation according to the visual analogue scale. The group of traumatic patients counted with 48 individuals, and presented with longer period of formal education (8,1 &#117; 4,1 x 5,3 &#117; 2,9 years; p < 0,001), higher count of tender points (16,1 &#117; 2,8 x &#117; 3,2; p = 0,047), shorter period since generalization of pain (5,6 &#117; 6,9 x 4,1 &#117; 2,6 years; p = 0,002), smaller prevalences of concentration difficulties (83,0% x 65,1%; p = 0,048) and abdominal cramps(45,8% x 26,4%; p = 0,036). There was an association between trauma and work disability. Any of remaining clinical aspects presented with statistically significant differences. We conclude that trauma imposes few clinical differences to fibromyalgia patients and does not explain the degree of work disability which is observed among them.

Células mesenquimais indiferenciadas humanas foram obtidas por digestão enzimática e centrifugação do produto de lipoaspiração, expandidas in vitro, e implantadas no tecido subcutâneo de camundongos atímicos. No grupo I, cada animal recebeu o implante de uma membrana de 0,25cm2 de ácido glicólico e carbonato de trimetileno semeada com 1 x 106 destas células .No grupo II, cada um recebeu a injeção de 0,2ml de gel de ácido hialurônico reticulado contendo o mesmo número destas células. Com três semanas de implante, células humanas e vasos foram identificados nos dois carreadores. Entretanto, com oito semanas, somente no gel de ácido hialurônico as células humanas e os vasos estavam presentes / Human undifferentiated mesenchymal cells were obtained by enzymatic digestion and centrifugation of the product of liposuction. These cells were expanded, in vitro, and implanted subcutaneously in athymic mice. In group I, each animal received the implant of a 0,25cm2 membrane of glycolic acid and trimethylene carbonate, seeded with 1 x 106 of these cells. In group II, each one received 0,2 ml of cross-linked hyaluronic acid gel containing the same amount of these cells. With three weeks of implantation, human cells and vessels were identified in both carriers. However, with eight weeks of implantation, only in hyaluronic acid gel human cells and vessels were present

Made available in DSpace on 2016-03-15T19:35:52Z (GMT). No. of bitstreams: 1 DENIS PAVEL CABEZAS HUAMAN.pdf: 1718725 bytes, checksum: b98f52c6b4dac9b37a33cc5bc62d871e (MD5) Previous issue date: 2015-03-26 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The project aims to study the disturbances (responses) of the low solar atmosphere caused by solar flares, using H&#945;, 30 THz (10 &#956;m), UV/EUV, soft and hard X-rays observations, and a wide range of radio waves emissions (microwaves, millimeter, sub-millimeter). This set of multispectral data enabled us to (i) examine in detail various aspects of the phenomena, (ii) determine the origin of the radio emission during the gradual phase, and therefore (iii) understand the mechanism of the particles acceleration. The data we used for the completion of this study are based on H&#945; and 30 THz (10 &#956;m) observations, made by the high cadence flare imaging system installed at OSM4 and CASLEO5 observatories, additionally data from HASTA (H-Alpha Solar Telescope for Argentina) telescope installed at OAFA6 observatory. In the sub-millimeter and millimeter domain at 212, 405 GHz and 45, 90 GHz, were from SST (Solar Sub-millimeter Telescope) and from POEMAS (POlarization Emission of Millimeter Activity at the Sun) solar radio telescopes, respectively. Complementary radio observations in the microwave range (0.2-15 GHz) from RSTN (Radio Solar Telescope Network) were considered. In addition, solar observations obtained by RHESSI (Reuven Ramaty High Energy Solar Spectroscopic Imager), Fermi and GOES (Geostationary Operational Environmental Satellite) satellites in X-ray channels, and from AIA (Atmospheric Imaging Assembly) and HMI (Helioseismic and Magnetic Imager) instruments, onboard the SDO (Solar Dynamics Observatory) satellite, respectively. / O trabalho tem como objetivo estudar as perturbações da baixa atmosfera solar produzidas pelas explosões solares, usando observações em H&#945;, 30 THz (10 &#956;m), UV/EUV, raios X e uma ampla faixa de emissões em ondas de rádio (microondas, milimétrica, submilimétrica); com o propósito de determinar a natureza dos processos físicos envolvidos. Este conjunto de dados multiespectrais nos permitiu (i) analisar os diferentes aspectos das explosões tanto na fase impulsiva como gradual, (ii) determinar a origem da emissão em rádio durante a fase gradual, e consequentemente (iii) entender melhor os mecanismos de aceleração das partículas. Os dados em H&#945; e 30 THz foram fornecidos pelo sistema de aquisição de dados com alta resolução temporal, instalado nos observatórios OSM1 e CASLEO2, adicionalmente dados do telescópio HASTA (H-Alpha Solar Telescope for Argentina) instalado no observatório OAFA3. Enquanto as observações em rádio, os dados foram do rádio polarímetro POEMAS (POlarization Emission of Millimeter Activity at the Sun) nas frequências de 45 e 90 GHz, do telescópio SST (Sub-millimeter Solar Telescope) nas frequências 212 e 405 GHz, ambos instalados no CASLEO. Dados na faixa de microondas (0,2-15 GHz) obtidos pela rede RSTN (Radio Solar Telescope Network). Além disso, foram considerados observações do Sol obtidas pelos satélites RHESSI (Reuven Ramaty High Energy Solar Spectroscopic Imager), Fermi e GOES (Geostationary Operational Environmental Satellite) para raios X, e dos instrumentos AIA (Atmospheric Imaging Assembly), HMI (Helioseismic and Magnetic Imager), a bordo do satélite SDO (Solar Dynamics Observatory), respectivamente.

Master of Philosophy (MPhil) / Informationist Science Fiction theory provides a way of analysing science fiction texts and narratives in order to demonstrate on an informational basis the uniqueness of science fiction proper as a mode of fiction writing. The theoretical framework presented can be applied to all types of written texts, including non-fictional texts. In "Informationist Science Fiction Theory and Informationist Science Fiction" the author applies the theoretical framework and its specific methods and principles to various contemporary science fiction works, including works by William Gibson, Neal Stephenson and Vernor Vinge. The theoretical framework introduces a new informational theoretic re-framing of existing science fiction literary theoretic posits such as Darko Suvin's novum, the mega-text as conceived of by Damien Broderick, and the work of Samuel R Delany in investigating the subjunctive mood in SF. An informational aesthetics of SF proper is established, and the influence of analytic philosophy - especially modal logic - is investigated. The materialist foundations of the metaphysical outlook of SF proper is investigated with a view to elucidating the importance of the relationship between scientific materialism and SF. SF is presented as The Fiction of Veridical, Counterfactual and Heterogeneous Information.

Master of Philosophy (MPhil) / Informationist Science Fiction theory provides a way of analysing science fiction texts and narratives in order to demonstrate on an informational basis the uniqueness of science fiction proper as a mode of fiction writing. The theoretical framework presented can be applied to all types of written texts, including non-fictional texts. In "Informationist Science Fiction Theory and Informationist Science Fiction" the author applies the theoretical framework and its specific methods and principles to various contemporary science fiction works, including works by William Gibson, Neal Stephenson and Vernor Vinge. The theoretical framework introduces a new informational theoretic re-framing of existing science fiction literary theoretic posits such as Darko Suvin's novum, the mega-text as conceived of by Damien Broderick, and the work of Samuel R Delany in investigating the subjunctive mood in SF. An informational aesthetics of SF proper is established, and the influence of analytic philosophy - especially modal logic - is investigated. The materialist foundations of the metaphysical outlook of SF proper is investigated with a view to elucidating the importance of the relationship between scientific materialism and SF. SF is presented as The Fiction of Veridical, Counterfactual and Heterogeneous Information.