Yes / Background: Melanin is synthesized by melanocytes in the basal layer of the epidermis. When transferred to surrounding keratinocytes it is the key UVR-protective biopolymer responsible for skin pigmentation. Most melanin is observable in the proliferative basal layer of the epidermis, and only sparsely distributed in the stratifying/differentiating epidermis. The latter has been explained, despite formal evidence, to ‘melanin degradation’ in supra-basal layers. Objectives: Our aim was to re-evaluate this currently-accepted basis for melanin distribution in the human skin epidermis, and whether this pattern is altered after a regenerative stimulus. Methods: Normal epidermis of adult human skin, at rest and after tape-stripping, was analysed by a range of (immuno)histochemical and high-resolution microscopy techniques. In vitro models of melanin granule uptake by human keratinocytes were attempted. Results: We propose a wholly different fate for melanin in the human epidermis. Our evidence indicates that the bulk of melanin is inherited only by the non-differentiating daughter cell post mitosis in progenitor keratinocytes, via asymmetric organelle inheritance. Moreover, this preferred pattern of melanin distribution can switch to a symmetric or equal daughter cell inheritance mode under conditions of stress including regeneration. Conclusions: We provide in this preliminary report a plausible and histologically-supportable explanation for how human skin pigmentation is efficiently organized in the epidermis. Steady state epidermis pigmentation may involve much less redox-sensitive melanogenesis than previously thought, and at least some pre-made melanin may be available for re-use. The epidermal-melanin unit may be an excellent example to study organelle distribution via asymmetric or symmetric inheritance in response to micro-environment and tissue demands. / Walgreens Boots Alliance

Thesis (Ph. D.)--University of California, San Diego, 2009. / Title from first page of PDF file (viewed June 25, 2009). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references (p. 296-311).

This study seeks to confront the current Filipino cultural identity by investigating whiteness within the mass media context there.

Subjects were 101 Mexican-American adults (53 females, 48 males), age range 17-72, and most often were in the blue-collar job level. Instructions were that (a) 18 pairs of slides would be shown; (b) each slide would be projected for 15 seconds; (c) each of the two models was to be judged on intelligence, attractiveness, friendliness, happiness, and success; and (d) the rating scale would be marked corresponding to the left or right slide. Results indicated the lighter-skinned models were judged more favorably than the darker ones on all five dimensions. To the extent this study sheds light on an important cultural value, it is hoped the treatment of Mexican-Americans in therapy will be facilitated and improved.

During tissues development multipotent progenitor cells establish tissue-specific gene expression programmes, leading to differentiation into specialized cell types. It has been previously shown that the transcription factor p63, a master regulator of skin development, controls the expression of adhesion molecules and essential cytoskeleton components. It has also been shown that p63 plays an important role in establishing distinct three-dimensional conformations in the Epidermal Differentiation Complex (EDC) locus (Fessing et al., 2011). Here we show that in p63-null mice about 32% of keratinocytes showed altered nuclear morphology. Alterations in the nuclear shape were accompanied by decreased expression of nuclear lamins (Lamin A/C and Lamin B1), proteins of the LINC complex (Sun-1, nesprin-2/3) and Plectin. Plectin links components of the nuclear envelope (nesprin-3) with cytoskeleton and ChIP-qPCR assay with adult epidermal keratinocytes showed p63 binding to the consensus binding sequences on Plectin 1c, Sun-1 and Nesprin-3 promoters. As a possible consequence of the altered expression of nuclear lamins and nuclear envelope-associated proteins, changes in heterochromatin distribution as well as decrease of the expression of several polycomb proteins (Ezh2, Ring1B, Cbx4) has been observed in p63-null keratinocytes. Moreover, recent data in our lab have showed that p63 directly regulates Cbx4, a component of the polycomb PRC1 complex. Here we show that mice lacking Cbx4 displayed a skin phenotype, which partially resembles the one observed in p63-null mice with reduced epidermal thickness and keratinocyte proliferation. All together these data demonstrate that p63-regulated gene expression program in epidermal keratinocytes includes not only genes encoding adhesion molecules, cytoskeleton proteins (cytokeratins) and chromatin remodelling factors (Satb1, Brg1), but also polycomb proteins and components of the nuclear envelope, suggesting the existence of a functional link between cytoskeleton, nuclear architecture and three dimensional nuclear organization. Other proteins important for proper epidermal development and stratification, are cytokeratins. Here, we show that keratin genes play an essential role in spatial organization of other lineage-specific genes in keratinocytes during epidermal development. In fact, ablation of keratin type II locus from chromosome 15 in epidermal keratinocytes led to changes in the genomic organization with increased distance between the Loricrin gene located on chromosome 3 as well as between Satb1 gene located on chromosome 17 and keratin type II locus, resulting in a more peripheral localization of these genes in the nucleus. As a possible consequence of their peripheral localization, reduced expression of Loricrin and Satb1 has also been observed in keratins type II-deficient mice. These findings together with recent circularized chromosome conformation capture (4C) data, strongly suggest that keratin 5, Loricrin and Satb1 are part of the same interactome, which is required for the proper expression of these genes and proper epidermal development and epidermal barrier formation. Taken together these data suggest that higher order chromatin remodelling and spatial organization of genes in the nucleus are important for the establishment of lineage-specific differentiation programs in epidermal progenitor cells. These data provide an important background for further analyses of nuclear architecture in the alterations of epidermal differentiation, seen in pathological conditions, such as psoriasis and epithelial skin cancers.

Thesis (M.Fine Arts)--University of the Witwatersrand, Faculty of Humanities, School of Arts, 2016. / This paper will be an analysis of the covers and contents of the South African editions of Glamour magazine from September 2014 to August 2015 and True Love magazine from September 2014 to August 2015. The analysis will consider the effects of: globalisation; globalised culture and consumption; and perceptions of race and skin colour, (specifically the notion of colourism) in South Africa. Colourism is a prejudicial system that renders value and perpetuates social hierarchies along perceived tonal difference in skin colour. It has been asserted by writers like Deborah Gabriel and Nicole Fleetwood that this value system exists within communities of people of colour and is perpetuated by mainstream media, but maintains a somewhat obscure presence. I will consider the mechanisms that inform this colour system and will show how globalisation works to facilitate colourism. Finally, I aim to explain how skin colour extends beyond the body and define the effects of global cultural interaction, showing that colourism is not simply about skin colour and tone, but about economic, social, and political realities. / MT2017

Although an iontophoretic trans-dermal drug delivery is known as an effective means for drug transportation through the human skin, it is not widely used because of the various side effects that come to life due to a high applied voltage of up to 80V. This study introduces an alternative means of drug transportation through the skin by means of sweat gland activation and reduction of an applied voltage to ensure that the iontophoresis is safe. The skin conductance studies performed on the pulmar area using 50mM of NaCl showed that the activation of sweat glands led to the increase of the skin conductance up to 8-10 times which enabled us to use a lower voltage of 2V in order to achieve noticeable results during the actual drug delivery experiment performed in the points of low ionic resistance that are located on a human biceps, also the application of Vaseline on the experimental surface does not allow the decrease of a skin conductance for as long as 11 hours which enables us to do the drug delivery over a long period of time. Finally, the drug delivery was performed and tested by means of HPLC method.

La connaissance des propriétés mécaniques de la peau humaine in vivo est d’une importance capitale dans de nombreux domaines (médical, cosmétique…). L’objectif de cette thèse est de développer de nouveaux outils pour permettre d’une part au clinicien de caractériser de manière objective les propriétés mécaniques de la peau, et d’autre part d’améliorer la compréhension générale du comportement de cet organe avec le vieillissement. Le premier chapitre est une revue bibliographique de la physiologie et des propriétés physiques de la peau ainsi que des différents moyens d’investigations actuels de ses propriétés. A partir de cette revue, deux axes de recherche sont définis, un axe de sollicitation tangentielle et un axe de sollicitation normale au tissu. Le second chapitre s’intéresse au premier axe de recherche, avec le développement d’une méthode d’indentation dynamique et d’une méthode d’indentation sans contact. Une baisse du module d’Young est trouvée avec l’âge. Le troisième chapitre s’intéresse à l’axe de sollicitation tangentielle, avec une méthode d’extension compression couplant mesures d’efforts et mesures des champs de déplacements de la zone sollicitée. Une approche inverse par un modèle Éléments Finis avec une loi de comportement orthotrope montre à partir des essais réalisés une baisse globale des propriétés mécaniques de la peau avec l’âge. Le quatrième et dernier chapitre relie les deux approches (normale et tangentielle) en comparant les résultats obtenus et tire les perspectives de ces travaux. / The knowledge of the mechanical properties of human skin in vivo is essential for many domains (medical, clinical…). The aim of this thesis is to develop new devices for the clinician in order to perform objective assessment of the mechanical properties of human skin, and also to improve the understanding of the whole mechanical behaviour of this organ with ageing.The first chapter is a bibliography concerning the physiology and the physical properties of the skin and also an overview of the actual devices used for the assessment of these properties. Based on this review, two different ways of stress have been chosen, a normal stress axis and a tangential stress axis to the skin.The second chapter concerns the first way of stress, with the development of a dynamic indentation method and a non contact method. A decrease of the Young modulus is found with ageing.The third chapter concerns a tangential axis of stress, with an extension-compression test using force measurement combined to displacement field measurement of the stressed area. An inverse method using a Finite Element model with an orthotropic law shows that results obtained give a decrease of the mechanical properties of the skin with ageing.The fourth and last chapter links the two different way of stress used with a comparison of results obtained and gives some perspectives of this work.

Automated facial metrology is the science of objective and automatic measurement of the human face. There are many reasons for measuring the human face. Psychologists are interested in determining how humans perceive beauty, and how this is related to facial symmetry [158]. Biologists are interested in the relationship between symmetry and biological fitness [124]. Anthropologists, surgeons, forensic experts, and security professionals can also benefit from automated facial metrology [32, 101, 114]. This thesis investigates the concept of automated facial metrology, presenting original techniques for segmenting 3D range and colour images of the human head, measuring the bilateral symmetry of n-dimensional point data (with particular emphasis on measuring the human head), and extracting the 2D profile of the face from 3D data representing the head. Two facial profile analysis techniques are also presented that are incremental improvements over existing techniques. Extensive literature reviews of skin colour modelling, symmetry detection, symmetry measurement, and facial profile analysis are also included in this thesis. It was discovered during this research that bilateral symmetry detection using principal axes is not appropriate for detecting the mid-line of the human face. An original mid-line detection technique that does not use symmetry, and is superior to the symmetry-based technique, was developed as a direct result of this discovery. There is disagreement among researchers about the effect of ethnicity on skin colour. Some researchers claim that people from different ethnic groups have the same skin chromaticity (hue, saturation) [87, 129, 206], while other researchers claim that different ethnic groups have different skin colours [208, 209]. It is shown in this thesis that people from apparently different ethnic groups can have skin chromaticity that is within the same Gaussian distribution. The chromaticity-based skin colour model used in this thesis has been chosen from the many models previously used by other researchers, and its applicability to skin colour modelling has been justified. It is proven in this thesis that the Mahalanobis distance to the skin colour distribution is Gaussian in both the chromatic and normalised rg colour spaces. Most facial profile analysis techniques use either tangency or curvature to locate anthropometric features along the profile. Techniques based on both approaches have been implemented and compared. Neither approach is clearly superior to the other, but the results indicate that a hybrid technique, combining both approaches, could provide significant improvements. The areas of research most relevant to facial metrology are reviewed in this thesis and original contributions are made to the body of knowledge in each area. The techniques, results, literature reviews, and suggestions presented in this thesis provide a solid foundation for further research and hopefully bring the goal of automated facial metrology a little closer to being achieved.

Lic.-avh. (sammanfattning) Stockholm : Karolinska institutet, 2006. / Härtill 3 uppsatser.