This study advances and adds detail to our history of the reading of verse in England c.1350-1500. Scholarship has established major twelfth- and thirteenth-century changes in reading, and linked these changes to manuscripts containing the modern Middle English verse canon. Historians of early modern reading have also argued for distinctive changes in their own period. But the examination of reading between these two clusters of change has been limited. This study therefore asks how later medieval Middle English verse was read. The surviving copies of The Prick of Conscience and Speculum Vitae, two hugely successful religious instructional poems, form the primary body of evidence. This body is augmented by reference to hundreds of other manuscripts containing Middle English verse. Together, these can reveal much about what was normal and abnormal in reading. They are also an important part of the context for the reading of more canonical Middle English verse. Manuscript studies often proceeds through case studies of individual books and unusual evidence such as marginalia. This thesis turns to codicology to understand more widespread evidence for reading, combining qualitative case studies with quantitative techniques borrowed and developed from continental scholarship. The first chapter examines evidence of provenance, revealing that both The Prick of Conscience and Speculum Vitae were read by an impressive range of people and remained current into the sixteenth century. The second chapter considers the navigational aids used in copies of both poems. Reading in this period has been characterised as 'discontinuous', but it could be discontinuous in diverse ways, and readers also read continuously. The third chapter is a large-scale study of books' size and shape, showing how these features can reveal books' reading histories, sometimes in counterintuitive ways. The fourth chapter contends that readers in this period attended closely to rhyme and probably read for balanced rhyme structures. The fifth chapter uncovers the ways in which these poems were rewritten for new readers and investigates the composition of the Southern Recension of The Prick of Conscience, arguing that this new text was partly a formalist intervention. The conclusion summarises the new 'baseline' history of the reading of Middle English verse which is offered here, and gestures towards implications for our reading of the Middle English poems which are canonical today.

Literary texts of medieval England feature trees as essential to the individual and communal identity as it intersects with nature, and the compelling qualities and organic processes associated with trees help vernacular writers interrogate the changing nature of this character. The early depiction of trees demonstrates an intimacy with nature that wanes after the tenth-century monastic revival, when the representation of trees as living, physical entities shifts toward their portrayal as allegorical vehicles for the Church's didactic use. With the emergence of new social categories in the late Middle Ages, the rhetoric of trees moves beyond what it means to forge a Christian identity to consider the role of a ruler and his subjects, the relationship between humans and nature, and the place of women in society. Taking as its fundamental premise that people in wooded regions develop a deep-rooted connection to trees, this dissertation connects medieval culture and the physical world to consider the variety of ways in which Anglo-Saxon and post-Norman vernacular manuscripts depict trees. A personal identification with trees, a desire for harmony between society and the environment, and a sympathy for the work of trees lead to the narrator's transformation in the Dream of the Rood. The Tree of Knowledge of Good and Evil in the Junius 11 manuscript, illustrated in Genesis A, Genesis B, and manuscript images, scrutinizes the Anglo-Saxon Christian's relationship and responsibility to God in the aftermath of the Fall. As writers transform trees into allegories in works like Genesis B and Geoffrey Chaucer's Parson's Tale, the symbolic representations retain their spontaneous, organic processes to offer readers a visual picture of the Christian interior-the heart. Whereas the Parson's Tale promotes personal and radical change through a horticultural narrative starring the Tree of Penitence and Tree of Vices, Chaucer's Knight's Tale appraises the role of autonomous subjects in a tyrannical system. Forest laws of the post-Norman period engender a bitter polemic about the extent of royal power to appropriate nature, and the royal grove of the Knight's Tale exposes the limitations of monarchical structures and masculine control and shapes a pragmatic response to human failures.

Cloud computing has a significant impact on information and communication technology (ICT) and is one of the most important technological drivers of the digitalization of enterprises. However, due to the increasing dissemination of cloud services and the growing number of cloud service providers (CSPs), the uncertainty and risks for user companies in adopting cloud services have also increased. In this paper, we address those aspects from the perspective of the CSPs. We identified relevant literature and studies and conducted interviews with business experts from 16 German CSPs. In our results, we present current customer requirements and barriers to using cloud services from a provider’s viewpoint and identify the actions of and obstacles for CSPs in meeting the needs and constraints of the customers. Finally, we identify current and future challenges for CSPs in dealing with customer requirements and barriers by addressing their root causes. One of the main challenges from the CSPs’ perspective is addressing customers appropriately and building relationships of trust. This also “forces” changes in the sales processes. In this process, the essential challenges can be identified as an increase in complexity and a simultaneous simplification of specific sales activities. Therefore, the necessity arises for the continuous support of business relationships through value-adding and additional services. However, this results in another challenge for the CSPs – Namely, to find the right balance between standardization and meeting customer-specific requirements. In our paper, we show that the perspective of the CSPs is rarely discussed in the literature. Nevertheless, understanding the perceptions of the providers and their actions and measures is essential for future research activities in the field of cloud service selection. Comparing the customers’ perspectives and viewpoints with the CSPs’ actions will enhance the development of a holistic selection approach for future cloud projects. Therefore, our paper’s contribution to research is also the identification of this missing integration.

This thesis explores the relationship between Creation and the saints Cuthbert and Guthlac in their Anglo-Latin and Old English vitae. It argues that this relationship is best understood through received theological exegesis concerning Creation's present state in the postlapsarian world. The exegesis has its foundation in Augustine's interpretations of the Genesis narrative, though it enters the textual tradition of the vitae via an adapted portion of De Genesi contra Manichaeos in Bede's metrical Vita Sancti Cuthberti (VCM). Both Augustine and Bede argue, with slight differences, that fallen Creation can be restored into prelapsarian harmony with humanity by way of sanctity. Each individual vita engages with this understanding of the Fall in distinct, though ultimately interrelated, ways, and the chapters of this thesis will therefore explore each text individually. Chapter 1 argues that the anonymous Vita Sancti Cuthberti (VCA) unites Cuthbert's ability to restore Creation with the theme of monastic obedience, linking the ordering of a monastery to the restoration of prelapsarian harmony. The VCA also seeks to create sites for potential lay pilgrimage in the landscapes of Farne and Lindisfarne by highlighting the present efficacy of Cuthbert's miracles. Chapter 2 argues that Bede's VCM not only reveals his early attempt to fashion Cuthbert into the primary saint for Britain, via a focus on Cuthbert's obedience to the Divine Office, but also that the restoration of Creation functions as a ruminative tool. Chapter 3 argues that Bede transforms the nature of Cuthbert's sanctity in his prose Vita Sancti Cuthberti (VCP) from static to developmental, influenced by the Evagrian Vita Antonii, and that Creation is adapted to function as the impetus for, and evidence of, Cuthbert's progression. Chapter 4 argues that Felix's Vita Sancti Guthlaci (VSG) unites the development of Guthlac with a physically delineated Creation, and that the restoration of Creation is elevated to an even greater degree here than in Bede's hagiography. Chapter 5 argues that the author of the Old English Prose Guthlac (OEPG) grounds his vita by utilizing a landscape lexis shared with contemporary boundary clauses, so that here the relationship between the saint and Creation has greater force; it further argues that Guthlac A uniquely connects Guthlac with the doctrine of replacement, consolidating links between his arrival to the eremitic space and the restoration of prelapsarian Eden.

Surfaces of carbon fibre roving were modified by means of a low temperature plasma treatment to improve their bonding with mineral fines; the latter serving as an inorganic fibre coating for the improved mechanical performance of carbon reinforcement in concrete matrices. Variation of the plasma conditions, such as gas composition and treatment time, was accomplished to establish polar groups on the carbon fibres prior to contact with the suspension of mineral particles in water. Subsequently, the rovings were implemented in a fine concrete matrix and their pull-out performance was assessed. Every plasma treatment resulted in increased pull-out forces in comparison to the reference samples without plasma treatment, indicating a better bonding between the mineral coating material and the carbon fibres. Significant differences were found, depending on gas composition and treatment time. Microscopic investigations showed that the samples with the highest pull-out force exhibited carbon fibre surfaces with the largest areas of hydration products grown on them. Additionally, the coating material ingresses into the multifilament roving in these specimens, leading to better force transfer between individual carbon filaments and between the entire roving and surrounding matrix, thus explaining the superior mechanical performance of the specimens containing appropriately plasma-treated carbon roving.

Detection and quantification of small peptides, such as yeast pheromones, are often challenging. We developed a highly sensitive and robust affinity-assay for the quantification of the α-factor pheromone of Saccharomyces cerevisiae based on recombinant hydrophobins. These small, amphipathic proteins self-assemble into highly stable monolayers at hydrophilic-hydrophobic interfaces. Upon functionalization of solid supports with a combination of hydrophobins either lacking or exposing the α-factor, pheromone-specific antibodies were bound to the surface. Increasing concentrations of the pheromone competitively detached the antibodies, thus allowing for quantification of the pheromone. By adjusting the percentage of pheromone-exposing hydrophobins, the sensitivity of the assay could be precisely predefined. The assay proved to be highly robust against changes in sample matrix composition. Due to the high stability of hydrophobin layers, the functionalized surfaces could be repeatedly used without affecting the sensitivity. Furthermore, by using an inverse setup, the sensitivity was increased by three orders of magnitude, yielding a novel kind of biosensor for the yeast pheromone with the lowest limit of detection reported so far. This assay was applied to study the pheromone secretion of diverse yeast strains including a whole-cell biosensor strain of Schizosaccharomyces pombe modulating α-factor secretion in response to an environmental signal.

While many riddles exist in the Anglo-Saxon Exeter Book containing female characters, both as actual human females and personified objects and aspects of nature, few scholars have discussed how the anthropomorphized “females” of the riddles challenge and broaden more conventional portrayals of what it meant to be “female” in Anglo-Saxon literature. True understanding of these riddles, however, comes only with this broader view of female, a view including a mixture of ferocity and nobility of purpose and character very reminiscent of the valkyrie (OE wælcyrige), a figure mentioned only slightly in Anglo-Saxon literature, but one who deserves more prominence, particularly when evaluating the riddles of the Exeter Book and two poems textually close to the riddles, The Wife's Lament and Wulf and Eadwacer, the only two poems with a female voice in the entire Old English corpus. Riddles represent culture from a unique angle. Because of their heavy dependence upon metaphor as a vehicle or disguise for the true subject of the riddle, the poet must employ a metaphor with similar characteristics to the true riddle subject, or the tenor of the riddle. As the riddle progresses, similarities between the vehicle and the tenor are listed for the reader. Within these similarities lie the common ground between the two objects, but the riddle changes course at some point and presents a characteristic the vehicle and tenor do not have in common, which creates a gap. This gap of similarities must be wide enough for the true solution to appear, but not so wide so that the reader cannot hope to solve the mental puzzle. Because many of the riddles of the Exeter Book involve women and portrayal of objects as “female,” it is important to analyze the use of “female” as a vehicle to see what similarities arise.

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Tunicata umfasst 3000 marine Arten, mit sehr unterschiedlichen Lebensstrategien. Als eines der drei großen Taxa innerhalb der Chordata, stellt die Evolution der Tunikaten eine Schlüsselkomponente bei der Aufklärung der Evolution der Chordaten und Cranioten dar. Dafür ist ein Verständnis der Merkmalstransformationen innerhalb der Tunikaten notwendig. Leider sind die internen Verwandtschaftsverhältnisse der fünf großen Tunikatentaxa in verschiedenen molekularphylogenetischen Studien widersprüchlich. Bisher gibt es nur wenige morphologische phylogenetische Analysen. Ein Schwerpunkt dieser Arbeit liegt auf der Untersuchung neuroanatomischer Merkmale, da das Nervensystem wahrscheinlich phylogenetische Informationen enthält. Durch das Anwenden moderner morphologischer Methoden, wie hochauflösende konfokale Laserscan- und Elektronenmikroskopie (REM und TEM), und 3d Rekonstruktionen basierend auf lichtmikroskopischen Schnitten, wurde die Verfügbarkeit neuroanatomischer Daten wesentlich verbessert. Die Ergebnisse zeigen, dass die Variation neuroanatomischer Merkmale größer ist als bisher angenommen und dass sich die Gehirnanatomie und die Verteilung von Neurotransmittern in den zwei Stadien der Thaliaceen unterscheidet. Neue unabhängige Merkmale des Nervensystems wurden in einer Matrix kodiert. Ergänzt mit traditionellen in der Tunikatentaxonomie verwendeten Merkmalen, entstand die bisher umfangreichste morphologische Datenmatrix, die 116 Merkmale für insgesamt 54 Arten umfasst. Die kladistische Analyse ergab monophyletische Tunicata, in denen die Appendicularia die Schwestergruppe der übrigen Tunikaten bildet. Ascidiacea ist monophyletisch, während „Thaliacea“ paraphyletisch ist. Zusätzlich wurde eine kombinierte phylogenetische Analyse basierend auf den morphologischen Daten und 18S rDNA-Sequenzen durchgeführt. Eine stufenweise stärkere Gewichtung phänotypischer Merkmale zeigt, dass die morphologischen Daten das Ergebnis der kladistischen Analyse stark beeinflussen. / Tunicata comprises 3000 marine species with diverse life-history strategies. As one of the three major chordate taxa, the evolution of tunicates plays a key role to elucidate chordate and craniate evolution. Therefore, a broader understanding of character transformations within tunicates is essential, but the interrelationships of the five main tunicate subtaxa in previous molecular phylogenetic analyses were contradictory. Morphological phylogenetic analyses are rare. In this comparative study emphasis was given to neuroanatomical characters, as the nervous system probably contains phylogenetic information. Applying modern morphological techniques like high-resolution confocal laser scanning microscopy and electron microscopy (SEM and TEM), serial sectioning for light microscopy, and digital 3d reconstruction, the number of available tunicate neuroanatomical data was considerably increased. It was revealed that the variation of neuroanatomical characters is higher than previously assumed, a specific pattern of serotonin-like immunoreactive cells in ascidians is present, and that brain anatomy and distribution of neurotransmitters in the two thaliacean life-cycle stages differs. Novel independent characters of the central nervous system were coded in a matrix for a cladistic analysis. Including traditional morphological from tunicate literature this effort resulted in the largest morphological data matrix to date, containing 116 phenotypic characters and 54 species. The cladistic analysis resulted in monophyletic Tunicata, with Appendicularia the sister taxon to the remaining tunicates. Furthermore, the monophyly of Ascidiacea is supported, whereas “Thaliacea” are paraphyletic. An additional phylogenetic analysis combining morphological and 18S rDNA-sequence data was performed. A reevaluation of this dataset with a successively increased weighting of the phenotypic data showed that morphological data strongly influence the outcome of the cladistic analysis.