I detta projektarbete har jag med utgångspunkt i duon Invader Ace utforskat hur vi kan använda oss av improvisation inom ramarna för elektronisk dansmusik. Istället för att producera elektronisk dansmusik med hjälp av dator, har vi med våra instrument och olika typer av tekniska ljudresurser undersökt vad som händer när vi skapar musiken i stunden genom improvisation som huvudverktyg. / <p>I texten har jag valt ut exempel som jag hoppas kan ge en inblick i vad detta projekt inneburit och resulterat i. Dessa finns dokumenterade på tillhörande skiva, eller via länkar i den digitala versionen av denna reflektion</p>

A enzima conversora de angiotensina (ECA) catalisa a conversão de angiotensina I (Ang I) no vasoconstritor angiotensina II (Ang II) e hidrolisa a bradicinina (BK). ECA somática (sECA) possui dois domínios homólogos (N e C) que têm 60% de identidade. Embora estas duas regiões tenham homologia grande, o sítio catalítico C-domínio exibe uma atividade três vezes maior do que o N-domínio na hidrolise de Ang I in vivo. Este fato torna interessante o desenvolvimento de novos estudos de inibidores ou a melhoria dos já existentes. O objetivo deste estudo foi obter a região Ala959 até Ser1066 do Cdomínio da sECA (c-sECA), em uma estrutura conformacional semelhante à estrutura nativa. Nós amplificamos a sequência correspondente ao sítio catalítico da c-sECA com 324pb e clonamos esta sequência no vetor pET 28a(+). O segmento (nomeado de pET28_c-sECA) foi expresso em sistema bacteriano. A proteína foi expressa na forma solúvel e a purificação foi feita em uma única etapa utilizando a coluna de afinidade His-tag, a qual produziu a proteína pura. Análises estruturais por dicroísmo circular e fluorescência confirmaram que a proteína recombinante estava na conformação correta, e os ensaios de atividade mostraram que a c-sECA possui atividade enzimática e é inibida por lisinopril. / The angiotensin-converting enzyme (ACE) catalyzes the conversion of angiotensin I (Ang I) to the vasoconstrictor angiotensin II (Ang II) and the hydrolysis of bradykinin (BK). Human somatic ACE (sACE) has two homologous domains (N and C) that show 60% identity. Although these two regions have high homology, the catalytic site of the C-domain exhibits three times more activity than that of the N-domain in the hydrolysis of Ang I in vivo. This fact necessitates the development of new inhibitors or the improvement of existing ones. This study aimed to obtain the Ala959 to Ser1066 catalytic region of C-domain of sACE (c-sACE) in a structural conformation that resembles the native structure. We amplified the 324-bp sequence corresponding to the catalytic site of c-sACE and cloned this sequence into a pET28a(+) vector. The segment (named pET28_c-sACE) was expressed in a bacterial system. The expressed protein segment was soluble, and its purification was performed in one step using a His-tag affinity column. Structural analysis by circular dichroism and fluorescence confirmed that the purified protein is correctly folded, and an activity assay showed that c-sACE possesses enzymatic activity and is inhibited by lisinopril.

A enzima conversora de angiotensina (ECA) catalisa a conversão de angiotensina I (Ang I) no vasoconstritor angiotensina II (Ang II) e hidrolisa a bradicinina (BK). ECA somática (sECA) possui dois domínios homólogos (N e C) que têm 60% de identidade. Embora estas duas regiões tenham homologia grande, o sítio catalítico C-domínio exibe uma atividade três vezes maior do que o N-domínio na hidrolise de Ang I in vivo. Este fato torna interessante o desenvolvimento de novos estudos de inibidores ou a melhoria dos já existentes. O objetivo deste estudo foi obter a região Ala959 até Ser1066 do Cdomínio da sECA (c-sECA), em uma estrutura conformacional semelhante à estrutura nativa. Nós amplificamos a sequência correspondente ao sítio catalítico da c-sECA com 324pb e clonamos esta sequência no vetor pET 28a(+). O segmento (nomeado de pET28_c-sECA) foi expresso em sistema bacteriano. A proteína foi expressa na forma solúvel e a purificação foi feita em uma única etapa utilizando a coluna de afinidade His-tag, a qual produziu a proteína pura. Análises estruturais por dicroísmo circular e fluorescência confirmaram que a proteína recombinante estava na conformação correta, e os ensaios de atividade mostraram que a c-sECA possui atividade enzimática e é inibida por lisinopril. / The angiotensin-converting enzyme (ACE) catalyzes the conversion of angiotensin I (Ang I) to the vasoconstrictor angiotensin II (Ang II) and the hydrolysis of bradykinin (BK). Human somatic ACE (sACE) has two homologous domains (N and C) that show 60% identity. Although these two regions have high homology, the catalytic site of the C-domain exhibits three times more activity than that of the N-domain in the hydrolysis of Ang I in vivo. This fact necessitates the development of new inhibitors or the improvement of existing ones. This study aimed to obtain the Ala959 to Ser1066 catalytic region of C-domain of sACE (c-sACE) in a structural conformation that resembles the native structure. We amplified the 324-bp sequence corresponding to the catalytic site of c-sACE and cloned this sequence into a pET28a(+) vector. The segment (named pET28_c-sACE) was expressed in a bacterial system. The expressed protein segment was soluble, and its purification was performed in one step using a His-tag affinity column. Structural analysis by circular dichroism and fluorescence confirmed that the purified protein is correctly folded, and an activity assay showed that c-sACE possesses enzymatic activity and is inhibited by lisinopril.

Submitted by FERNANDA DA SILVA VON PORSTER (fdsvporster@univates.br) on 2015-08-10T18:16:25Z No. of bitstreams: 3 license_text: 21490 bytes, checksum: 7ff346999f7486617a4302c4f2f02bc7 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) 2014LuanaMariaWollinger.pdf: 6093984 bytes, checksum: 2af11bb55c21559bc90e174f86d5d0b7 (MD5) / Approved for entry into archive by Ana Paula Lisboa Monteiro (monteiro@univates.br) on 2015-08-17T20:10:12Z (GMT) No. of bitstreams: 3 license_text: 21490 bytes, checksum: 7ff346999f7486617a4302c4f2f02bc7 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) 2014LuanaMariaWollinger.pdf: 6093984 bytes, checksum: 2af11bb55c21559bc90e174f86d5d0b7 (MD5) / Made available in DSpace on 2015-08-17T20:10:12Z (GMT). No. of bitstreams: 3 license_text: 21490 bytes, checksum: 7ff346999f7486617a4302c4f2f02bc7 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) 2014LuanaMariaWollinger.pdf: 6093984 bytes, checksum: 2af11bb55c21559bc90e174f86d5d0b7 (MD5) / Introdução: A Pressão Arterial (PA), bem como a Hipertensão Arterial Sistêmica (HAS), são fenótipos complexos onde fatores genéticos e ambientais influenciam na sua etiologia. Dentre os fatores genéticos, polimorfismos nos genes da Enzima Conversora de Angiotensina (ACE) e Angiotensinogêneo (AGT) vem sendo associados a estes desfechos. Em relação aos fatores ambientais, a dieta é um dos fatores de maior importância no processo evolutivo da doença, principalmente no que diz respeito ao consumo de sódio. Objetivo: Verificar se existe interação entre os polimorfismos Inserção/Deleção do gene ACE e rs699 do gene AGT e o consumo de micronutrientes (sódio, potássio, cálcio e magnésio) da dieta; e se esta interação influencia os valores de PA em uma amostra de brasileiros adultos saudáveis. Metodologia: Realizou-se um estudo do tipo transversal com 341 indivíduos brasileiros adultos de ambos os gêneros. Os valores de PA foram aferidos em equipamento digital marca Omron® modelo HEM-710INT. A análise dietética foi feita por meio do software Dietwin versão 2008 a partir do método de Recordatório Alimentar de 24 horas. A genotipagem do polimorfismo InsDel foi realizada através da Reação em Cadeia de Polimerase (PCR), com primers específicos, seguida de eletroforese em gel de agarose 1,5 %; o polimorfismo rs699 foi genotipado através do sistema de discriminação alélica TaqMan (Applied Biosystems). A Análise estatística foi realizada pelo uso do software Statistical Package for the Social Sciences (SPSS) versão 20.0. Resultados: As frequências genotípicas de ambos os polimorfismos estão em equilíbrio de Hardy-Weinberg. Em relação ao consumo dos micronutrientes, observou-se uma associação com o polimorfismo InsDel do gene ACE, onde indivíduos homozigotos Del/Del consomem menos cálcio do que os heterozigotos (Ins/Del) (p=0,007). Na comparação da PA entre os genótipos, verificou-se uma associação com o polimorfismo rs699 do gene AGT. Indivíduos homozigotos GG apresentaram maior PA Sistólica quando comparados aos indivíduos AA (p=0,028). As análises de interação indicaram uma associação entre o genótipo heterozigoto do polimorfismo rs699 do gene AGT no consumo de cálcio e magnésio na modulação dos valores de PA. Conclusão: Os polimorfismos dos genes ACE e AGT parecem estar associados com fatores do consumo alimentar e PA, assim como o gene AGT pode ter um papel importante nas relações nutrigenéticas nos desfechos relacionados a PA. / Introduction: The Blood Pressure (BP) and Hypertension are complex phenotypes, in which genetic and environment factors influence in the etiology. In the genetics factors, polymorphisms of the Angiotensin Converting Enzyme (ACE) and Angiotensinogen (AGT) gene, has been associated with on outcomes. In relation to environmental factors, the diet is a factor the most importance on evolutionary process of the disease, particularly with regard to the consumption of sodium. Objetive: To verificate if there is interaction between polymorphisms Insertion/Deletion (InsDel) of the ACE gene and rs699 of the AGT gene and the micronutrientes intake (sodium, potassium, calcium and magnesium) in the diet; and if there is interaction influences the BP values in the healthy brasilian sample. Metodology: Performed a cross-section study with 341 brazilian adult individuals of both genders. The BP values was measurement by digital equipment Omron® model HEM-710INT. The dietary analysis was assessed by the software Dietwin version 2008 from the 24-hour recall method. InsDel polymorphism genotyping was performed by Polymerase Chain Reaction (PCR), using specific primers, and analyzed on 1,5% agarose gel; the rs699 polymorphism was genotyped using the TaqMan SNP genotyping assays (Applied Biosystems). The statistical analysis was performe by the software Statistical Package for the Social Sciences (SPSS) version 20.0. Results: The genotype frequencies of both polymorphisms are balance Hardy-Weinberg. In relation micronutrients intake, our results show a association with InsDel polymorphism of the ACE gene, in which homozygotes Del/Del intake fewer calcium than heterozygotes (Ins/Del) (p=0,007). Comparing BP between genotypes, our results indicate a association with rs699 polymorphism of the AGT gene. Homozygotes GG showed greater Systolic BP than individuals AA (p=0,028). The interaction analysis indicate a association between heterozygote of the rs699 polymorphism and calcium and magnesium inatke in the modulation BP values. Conclusion: The polymorphisms of the ACE and AGT gene seem to be associated with factors of the food consumption and BP, as well as, the AGT gene may have an important role in nutrigenetics relationships on outcomes related to BP.

Within the United States, African Americans account for 13% of the entire population, making them the second largest minority group. A notable concern is the rate at which African Americans fail to utilize both medical and mental health services. Researchers have long examined possible factors, such as stigmas, barriers, and accessibility, as reasons why African Americans underutilize psychological help. Overlooked is the potential influence of adverse childhood experiences (ACE). The term ACE encompasses ten major domains, ranging from emotional abuse to parental divorce/separation, all of which have been found to negatively influence individuals’ well-being. As such, this study examined the association between African American women’s adverse childhood experiences and help seeking attitudes. Data for this study were gathered from 64 African American female college students. Adverse childhood experiences were measured with the Traumatic Experiences Checklist (TEC) and help seeking attitudes were assessed with the Attitudes Toward Seeking Professional Psychological Help-Short Form (ATSPPH-SF). Results demonstrated scores reflecting childhood traumatic events for violence, death, and legal involvement were negatively related to attitudes toward help seeking. The results of this study highlight the need for continued research regarding ACE for African American women in college settings. Specific implications for this study include outreach development in university counseling centers focused on the association between childhood trauma, violence/legal involvement, and intersectionality. Programming of this nature may provide a critical link to increase this population’s utilization of mental health services. Keywords: African American women, ACE, help seeking

The renin¡Vangiotensin system (RAS) plays a role in the pathogenesis of obesity. Angiotensin-converting enzyme (ACE), the component of the RAS system, has recently been found to be completely expressed in human adipose tissue. Angiotensin II, the active component of RAS, may affect adipogenesis and adipocyte metabolism. Among ACE polymorphism, the gene DD genotype has shown to be regulated with a higher agiotensin- converting enzyme level in plasma. Hence, the purpose of this research is to investigate the correlation of ACE gene polymorphism to body mass index (BMI) between aborigines and Han non-aborigines. The relationship of race and ACE insertion (I)/ deletion (D) polymorphism was also analyzed.The results showed a higher value of ACE DD genotype appeared in aborigines (35.7 %) than that in Han population (10.8 %)(p < 0.0001). BMI in aborigines was 26.4¡Ó4.6 kg/m2, while in Han population was 24.4¡Ó3.6 kg/m2 (P<0.0001). A higher waist circumference value was also found among females aborigines than that found among female in Han population (86.9¡Ó10.7 vs 84.3¡Ó9.7 cm, P<0.0001). Simple and multiple linear regression analyses showed that both race and ACE gene polymorphism are closely correlated to BMI in all subjects. By discussion on the cases for the Han and aborigines population separately, it was found that the ACE gene polymorphism is associated with BMI in Han population it is not significant in aborigines. In aborigines, life style in culture is associated with BMI. In conclusion, ACE gene polymorphism and race were independent factors correlated to BMI, but differences could be found between race and ACE gene polymorphism.

No description available.

Context: Although the long-term goal of antihypertensive therapy is to reduce adverse clinical outcomes, the only way to evaluate the efficacy of treatment in an individual is the magnitude of blood pressure (BP) reduction. ACE inhibitors and angiotensin receptor blockers (ARBs) are two drug classes that, by different mechanisms, inhibit the renin-angiotensin- aldosterone system that regulates BP. As these drugs are widely prescribed for hypertension, it is essential to determine and compare their effects on BP, heart rate and tolerability. Objectives: 1) To determine the dose-related effect of ACE inhibitors and ARBs on BP, heart rate and withdrawals due to adverse effects (WDAE), compared with placebo in the treatment of primary hypertension (SBP ≥ 140 mm Hg and/or ≥ DPB 90 mm Hg); and 2) To compare the relative effect on BP, heart rate and WDAE of a) each ACE inhibitor with other ACE inhibitors, b) each ARB with other ARBs, and c) all ACE inhibitors with all ARBs. Methods: Two systematic reviews of published, double-blind, randomized, controlled trials (RCTs) evaluating the BP lowering efficacy of fixed dose monotherapy with an ACE inhibitor or ARB compared with placebo for a duration of 3 to 12 weeks in patients with primary hypertension were conducted. Electronic databases were searched for RCTs and similar trial inclusion criteria and methods of analysis were used in both reviews. Results: Ninety two RCTs evaluated the dose-related BP lowering efficacy of 14 ACE inhibitors in 12 954 participants with a baseline BP of 157.1/101.2 mm Hg. Forty six RCTs evaluated the dose-related BP lowering efficacy of 9 ARBs in 13 451 participants with a baseline BP of 155.6/101.0 mm Hg. The best estimate of the near maximal trough BP reduction for ACE inhibitors and ARBs was -8/-5 mm Hg and -8/-5 mm Hg, respectively. ACE inhibitors and ARBs do not affect heart rate. The evidence for short-term WDAE (tolerability) was incomplete and weak and did not demonstrate a difference between the two classes of drugs. Conclusion: ACE inhibitors and ARBs are not different individually or as drug classes in BP lowering efficacy.

No description available.

Many bioactive peptides have been reported from various food proteins through the conventional activity-guided-purification approach; however, the rationale behind the selection of conditions for the production of the bioactive peptides has not been extensively explored. The purposes of the study were to provide the rationale behind the selection of conditions, and to develop an innovative strategy to explore the most potent peptides within egg proteins through an integrated QSAR and bioinformatics approach. Thermolysin-pepsin hydrolysate of ovotransferrin was predicted as the best condition for production of ACE-inhibitory peptides. Three predicted peptides, IRW, LKP and IQW, were successfully released from ovotransferrin. Simulated gastrointestinal incubation showed IQW was stable while IRW and LKP can be degraded into dipeptides (IR and KP respectively). Peptides produced from the study will have the potential to be developed as functional foods and nutraceuticals for the prevention of hypertension, a disease affecting ~ 31% of the adult population. / Food Science and Technology