Master of Science in Veterinary Science / A investigation into soil nutrient and the change soil physical properties was carried out for three pasture systems for dairy cows; the complementary forage rotation (CFR) which comprised of brassica (Brassica napus )/clover (Trifolium resupinatum ) and maize (Zea mays) capable of achieving more than 40 t/ha, the typical intensive pasture (PI) with kikuyu grass (Penisetum clandestinum) over-sown with ryegrass ( Lolium multiflorum ) and the extensive system (PE); on two soil types (brown chromosol and black vertisol) . The study demonstrated that the CFR system was capable of doubling its yield by using similar fertilizers (N, P and K) and water inputs compared to these of the PI system resulting of greater nutrients (N and K) and water use efficiencies. Overall, the nutrient lost through runoff water was very limited for two intensive systems and nearly nil for PE system. Also similar trends were observed for soil physical properties in the intensive systems except for the topsoil (0-10 cm) where light acidification (P= 0.04) was recorded in the CFR probably due to the soil works before the maize sowing.

Master of Science in Veterinary Science / A investigation into soil nutrient and the change soil physical properties was carried out for three pasture systems for dairy cows; the complementary forage rotation (CFR) which comprised of brassica (Brassica napus )/clover (Trifolium resupinatum ) and maize (Zea mays) capable of achieving more than 40 t/ha, the typical intensive pasture (PI) with kikuyu grass (Penisetum clandestinum) over-sown with ryegrass ( Lolium multiflorum ) and the extensive system (PE); on two soil types (brown chromosol and black vertisol) . The study demonstrated that the CFR system was capable of doubling its yield by using similar fertilizers (N, P and K) and water inputs compared to these of the PI system resulting of greater nutrients (N and K) and water use efficiencies. Overall, the nutrient lost through runoff water was very limited for two intensive systems and nearly nil for PE system. Also similar trends were observed for soil physical properties in the intensive systems except for the topsoil (0-10 cm) where light acidification (P= 0.04) was recorded in the CFR probably due to the soil works before the maize sowing.

Master of Science in Veterinary Science / A investigation into soil nutrient and the change soil physical properties was carried out for three pasture systems for dairy cows; the complementary forage rotation (CFR) which comprised of brassica (Brassica napus )/clover (Trifolium resupinatum ) and maize (Zea mays) capable of achieving more than 40 t/ha, the typical intensive pasture (PI) with kikuyu grass (Penisetum clandestinum) over-sown with ryegrass ( Lolium multiflorum ) and the extensive system (PE); on two soil types (brown chromosol and black vertisol) . The study demonstrated that the CFR system was capable of doubling its yield by using similar fertilizers (N, P and K) and water inputs compared to these of the PI system resulting of greater nutrients (N and K) and water use efficiencies. Overall, the nutrient lost through runoff water was very limited for two intensive systems and nearly nil for PE system. Also similar trends were observed for soil physical properties in the intensive systems except for the topsoil (0-10 cm) where light acidification (P= 0.04) was recorded in the CFR probably due to the soil works before the maize sowing.

Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniv., 2009. / Härtill 5 uppsatser.

The Late Rhaetian (Late Triassic) extinction event is characterised by shelled species showing a reduction in size, and thickness, which together with changed mineralogy is thought to be as a result of increased atmospheric pCO2 levels. Similar morphological changes have been demonstrated for extant species exposed experimentally to high CO2 leading to the hypothesis that Late Triassic extinctions were linked with global ocean acidification and increased oceanic palaeotemperatures. Consequently, the aim of this present work was to test this ocean acidification hypothesis by investigating morphological changes in selected shelled fossil species across this extinction event, and attempt to correlate them with changes in environmental temperature and pCO2. The abundance, size, shell thickness and mineralogy was determined for three common species, the bivalves Liostrea hisingeri and Plagiostoma gigantea and the ostracod Ogmoconchella aspinata collected from Triassic and Jurassic rocks from two locations in southwest England. Palaeotemperature was reconstructed from examination of these fossils and from the literature and atmospheric pCO2 estimated from published accounts. The shell size of bivalves increased during periods of high pCO2 and high palaeotemperature at both locations. Ostracod carapace sizes increased at St Audrie’s Bay but decreased at Lyme Regis during periods of high pCO2, while ostracod carapace size decreased during periods of high palaeotemperature at St Audrie’s Bay. However, ostracod shell thickness increased and decreased as pCO2 increased but shows no relationship with palaeotemperature at either location. Laboratory experiments on the effect of elevated pCO2 and elevated temperature on three modern species of ostracod was carried out. Modern species Leptocythere sp. and L. castanea subjected to either elevated pCO2 or elevated temperature showed increased dissolution, however size and thickness did not significantly change. In the same experimental conditions L. lacertosa showed increased dissolution however size continued to increase, while thickness was maintained. Comparison of fossil bivalve and ostracod data to modern high pCO2 and high temperature experiments illustrates some correlations to the modern experiments results indicating high pCO2 and high palaeotemperature conditions could have been occurring during the Triassic-Jurassic boundary interval. From the evidence presented, combined with an appropriate trigger (CAMP volcanism), it can be concluded that both ocean acidification and palaeotemperature were contributing to the species adaptations identified across the Triassic-Jurassic boundary interval.

The thesis assesses forms of phosphorus, aluminium, and iron in soils of catchments of acidified mountain lakes. The aim of the study was to determinate changes in soil chemistry in catchments of Bohemian Forest lakes between years 2000 and 2010. The next aim was to compare fractionation according to Psenner and Pucsko (1988) with standard pedological extractions.

The thesis assesses forms of phosphorus, aluminium, and iron in soils of catchments of acidified mountain lakes. The aim of the study was to determinate changes in soil chemistry in catchments of Bohemian Forest lakes between years 2000 and 2010. The next aim was to compare fractionation according to Psenner and Pucsko (1988) with standard pedological extractions.

Made available in DSpace on 2014-06-11T19:27:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-11-29Bitstream added on 2014-06-13T19:14:41Z : No. of bitstreams: 1 silva_lf_me_sjrp.pdf: 986291 bytes, checksum: e879f8c79aac66ac197646cb214878eb (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / No Brasil, o queijo Mussarela elaborado com leite de búfala, tem uma boa aceitação pelos consumidores e mercado em expansão. Entretanto, poucas são as pesquisas em âmbito nacional sobre a microbiota e influência das bactérias ácido-láticas utilizadas na produção, sobre a qualidade tecnológica deste queijo. O objetivo deste trabalho foi compor um banco de culturas representativo da microbiota isolada de queijo Mussarela fabricado com leite de búfala e efetuar a caracterização das bactérias ácido-láticas (BAL). Foram realizadas três coletas em dois laticínios (Laticínios A e B), em diferentes etapas do processo de fabricação, assim como no produto acabado (queijo Mussarela e soro de conservação) recém processado e com 14 e 28 dias de estocagem. Foi feita a contagem de colônias viáveis, isolamento dos mesófilos e termófilos, caracterização morfológica por coloração de Gram e teste de catalase. Foram obtidos 313 isolados que apresentaram características de BAL. As culturas isoladas das amostras do queijo do Laticínio B foram identificadas pela técnica de RAPD e sequenciamento do gene 16S rRNA e caracterizadas quanto à atividade acidificante, capacidade de utilizarem o citrato, atividade proteolítica e capacidade de produzirem compostos voláteis precursores de aromas. Para os dois laticínios, a população de microorganismos termófilos prevaleceu sobre os mesofilos. Os isolados foram identificados como Lactobacillus fermentum, Lactobacillus casei, Lactobacillus delbrueckii, Lactobacillus delbrueckii subsp. bulgaricus e Leuconostoc mesenteroides. A velocidade máxima de acidificação para os isolados variou de 0,0005 e 0,0305 unidades de pH por minuto após 20 min e 18 h 50 min do início do processo de fermentação, respectivamente para termófilos e mesófilos. O tempo... / In Brazil, the Mozzarella cheese prepared with buffalo milk has a good acceptance and market expansion. However, there are few national studies about microflora and influence of lactic acid bacteria, used in production, on the technological quality of this cheese. The aim this study was to compose a representative bank of the microbial cultures isolated from Mozzarella cheese produced with buffalo milk and to characterize the lactic acid bacteria (LAB). Three collections were performed in two dairy (Dairy A and B) at different stages of the manufacturing process as well as the finished product (Mozzarella cheese and whey conservation) newly processed and with 14 and 28 days of storage. It was followed a count of viable colony, isolation of mesophiles and thermophiles, morphological characterization by Gram staining and catalase test. It was obtained 313 isolates that exhibited characteristics of LAB. The cultures isolated of the cheese samples of the cheese from the Dairy B were identified by RAPD and 16S rRNA gene sequencing and characterized by acidifying activity, ability to utilize citrate, proteolytic activity and ability to produce volatile compounds that are flavor precursors. At two dairies, the population of thermophilic microorganisms was higher than mesophylic. The isolates were identified as Lactobacillus fermentum, Lactobacillus casei, Lactobacillus casei, Lactobacillus delbrueckii, Lactobacillus delbrueckii subsp. bulgaricus and Leuconostoc mesenteroides. The top speed of acidification for the isolates ranged from 0.0005 and 0.0305 pH units per minute after 20 minutes and 18:50 of the beginning of the fermentation process, respectively for thermophiles and mesophiles. The time required to reach the pH 5.0 ranged from 4h50min to 60h the beginning of... (Complete abstract click electronic access below)

Resumo: Muito tem se avaliado a respeito das propriedades físicas e químicas dos selantes de fóssulas e fissuras, mas pouco sobre seus efeitos no biofilme dentário. O objetivo deste estudo foi analisar as características bioquímicas e microbiológicas do biofilme dentário formado in situ sobre materiais que liberam flúor ou cálcio e fosfato. Dez voluntários utilizaram dispositivo intrabucal palatino contendo corpos-de-prova de materiais (Fuji IX GP, Auralay XF e Aegis) ou blocos de esmalte bovino. A fase experimental consistiu de 4 etapas de 8 dias cada e a utilização de sacarose para formação do biofilme. No 7º dia dos períodos experimentais foi determinado o pH do biofilme e no 8º dia, o biofilme dentário formado foi coletado para determinação de flúor (F), cálcio (Ca), fósforo (P) e polissacarídeos extracelulares insolúveis (PEI) além da microbiota. Verificou-se a liberação de F, Ca e P de cada material e dos blocos de esmalte após ciclagem de pH. Após a verificação da homogeneidade dos dados, testes paramétricos e não-paramétricos foram realizados para análise dos resultados (p<0,05). O biofilme dentário formado sobre os materiais que liberam flúor apresentou maior presença de F, Ca e P e menor número de estreptococos do grupo mutans quando comparado ao bloco de esmalte. Após o uso da sacarose (7º dia), o pH do biofilme foi maior na presença do material ionomérico. O biofilme sobre o material que contém fosfato de cálcio amorfo mostrou similar concentração de Ca e P, quando comparado aos materiais que contém flúor, e uma ação tamponante no meio, sem efeito na microbiota. Concluiu-se que os materiais que liberam flúor aumentaram as concentrações de F, Ca e P e diminuíram a de PEI e a microbiota cariogênica do biofilme dentário; e o que contém fosfato de cálcio amorfo aumentou as concentrações de Ca e P / Abstract: The chemical and physical properties of the pit and fissures sealants have been widely evaluated but rare researches focus on the effects on dental plaque. The aim of this study was to evaluate the biochemical and microbiological characteristics of the in situ dental plaque formed on materials that release fluoride or calcium and phosphate. Ten volunteers wore an intraoral palatal device containing specimens of materials (Fuji IX GP, Auralay XF and Aegis) or bovine enamel blocks. The experimental phase consisted of 4 stages with duration of 8 days each and use of sucrose to form the dental plaque. The dental plaque pH was determined at the 7th day of the experimental periods and the dental plaque was collected at the 8th day to determine the fluoride (F), calcium (Ca), phosphorus (P), insoluble extracellular polysaccharides (EPS) and microbiota. It was observed release of F, Ca and P from each material and from the enamel blocks after pH cycling. After verification of data homogeneity, parametric and non-parametric tests were performed (p<0.05). The dental plaque formed on the materials that release fluoride presented more F, Ca and P and reduced number of Streptococcus mutans in comparison to the enamel block. After use of sucrose (7th day), the dental plaque pH was higher with the presence of ionomeric material. The dental plaque on the material containing amorphous calcium phosphate exhibited similar Ca and P concentration compared to the ones on fluoride materials, and buffering action with no effect on the microbiota. It was concluded that the materials that release fluoride increased F, Ca and P concentrations, decreased EPS concentration and the cariogenic microbiota of the dental plaque; the amorphous calcium phosphate material increased concentrations of Ca and P / Orientador: Denise Pedrini / Coorientador: Alberto Carlos Botazzo Delbem / Banca: Cleide Cristina Rodrigues Martinhon / Banca: Stella Kossatz Pereira / Mestre

Structural changes in macrozoobenthic communities and changes in chemistry in longitudinal profiles of two acidified streams in the Bohemian Forest (Czech Republic) were investigated in 2007. The progress in chemical recovery was evident in both streams, whereas zoobenthos recovery was obvious only in less acidified stream.