Doctor of Philosophy / Department of Statistics / James Higgins / Many studies are performed on units that cannot be replicated due to cost or other restrictions. There often is an abundance of subsampling to estimate the within unit component of variance, but what is needed for statistical tests is an estimate of the between unit component of variance. There is evidence to suggest that the ratio of the between component of variance to the total variance will remain relatively constant over a range of studies of similar types. Moreover, in many cases this intraclass correlation, which is the ratio of the between unit variance to the total variance, will be relatively small, often 0.1 or less. Such situations exist in education, agriculture, and medicine to name a few. The present study discusses how to use such prior information on the intraclass correlation coefficient (ICC) to obtain inferences about differences among treatments in the face of no replication. Several strategies that use the ICC are recommended for different situations and various designs. Their properties are investigated. Work is extended to under-replicated experiments. The work has a Bayesian flavor but avoids the full Bayesian analysis, which has computational complexities and the potential for lack of acceptance among many applied researchers. This study compares the prior information ICC methods with traditional methods. Situations are suggested in which prior information ICC methods are preferable to traditional methods and those in which the traditional methods are preferable.

Master of Science / Department of Clinical Sciences / Daniel U. Thomson / Bovine respiratory disease complex (BRDC) is the most common, and costly, disease in feed yard cattle. A review of the literature shows a correlation between the diagnosis of BRDC ante-mortem and respiratory lesions at slaughter. The objectives of the studies reported here were to: 1) validate a thoracic auscultation scoring system by correlating ante-mortem lung sounds with post-mortem lung lesions and 2) evaluate thoracic auscultation and rectal temperature as diagnostic tools to predict case outcome in the feeder cattle treated for BRDC. First, a prospective cohort study involving thirty four head of cattle that had been realized from commercial cattle feeding operations were used to validate the use of a lung auscultation scoring system to identify cattle suffering from BRDC. Ante-mortem auscultation scores were compared to post-mortem lung lesions evaluated using a previously described scoring system. There was a positive correlation (P < .0001) between ante-mortem lung auscultation scores and post-mortem lung lesion scores in the population of feeder cattle that were tested. Subsequently, a retrospective cohort study was conducted using data obtained from three commercial feed yards. Cattle enrolled in the study (n = 4,341 head) were treated for BRDC between January 2007 to October 2007 by trained feed yard personnel. Data recorded included animal identification, rectal temperature, lung score, and antibiotic therapy at first treatment. Treatment outcome data were recorded by feed yard personnel utilizing an animal health computer. The outcome data tracked for this study included subsequent BRDC treatment or death of the animal. Our findings indicated that as lung auscultation score (P < .0001) or rectal temperature (P < .0001) increased there was an increased risk for cattle to require a second BRDC treatment. Also, we observed an increased risk for death loss in cattle with higher lung auscultation scores (P < .0001) or higher rectal temperature (P < .0001) at the time of treatment for BRDC. We have demonstrated that lung auscultation score and rectal temperature can be used as tools to predict treatment outcome in cattle treated for BRDC. Future research with these tools could be used to develop more precise therapeutic protocols for BRDC in feeder cattle.

Doctor of Philosophy / Department of Biological & Agricultural Engineering / James K. Koelliker / Small reservoirs catch and store water for long periods and they decrease streamflow and increase ground-water recharge. A field monitoring program provided the measured water depth for four years in several reservoirs in the Republican River Basin where there are concerns about their aggregate effects in the basin. The daily water budget operation for one reservoir was developed. Daily seepage rates were estimated by using precipitation, inflow and evaporation which was assumed equal to grass reference evapotranspiration (ET0), that average 120 to 150 cm/yr, along with the measured stage-storage and stage-surface area relationships. Two computer simulation modules, written in FORTRAN 95, were developed to estimate 1) overflow and gross seepage and 2) potential for ground-water recharge underneath the reservoir. Required daily input data are precipitation, ET0, and inflow from the watershed area. Required reservoir site characteristics include stage-storage and stage-surface area relationships, a standard seepage rate (S0) at 14 different levels in the reservoir, soil-water and plant-growth characteristics and a monthly crop-residue factor. The gross seepage module calculates water depth that determines daily overflow, the water-surface area for evaporation and the head of water on the 14 levels to cause seepage losses. If a level is not inundated, seepage is zero. If a level is inundated less than 0.3-m, S0 is used. When the water head (hL) on a level exceeds 0.3 m, the seepage rate (SL) is increased by, SL = S0 * (hL/0.3)0.25. This relationship was chosen after testing several exponent values between 0 and 1. The modules were calibrated on one reservoir and verified on two others in northwestern Kansas. Results showed runoff from the watersheds averaged about 1.2 to 1.6 cm/yr from the average annual precipitation of 46 to 62 cm. The three reservoirs reduced streamflow at the reservoir site by 74 to 97%, but 90 to 95% of the retained runoff was calculated to contribute to ground-water recharge. Several sensitivity analyses for model inputs were done. Results showed that, the ratio of the average annual inflow volume from the watershed area to the reservoir storage volume was the most sensitive input variable tested.

The historical geography of farming in the West Central Region of Scotland has been under-researched. Generalisations based on research relating to other parts of the country are misleading because the development and forms of agriculture in the West Central Region were distinctive. Traditionally this is an area of dairy farming which, during the research period (c.1850-1930) was characterised by small family labour farms. The concentration of small farms, on which the faming family and a few hired workers formed the core labour-force, and where the distinctions between employer and employed were less than on the large arable farms of the East, had consequences for rural social structure, mitigating the effects of capitalism. Through a small set of family labour farms, and the families associated with them, the thesis takes a grassroots approach to exploring the pattern of life on the farms of the Region, with particular regard to gender relations. The survival of such farms, contrary to Marxist expectations is investigated, along with the resilience of the farms during the period of ‘The Great Agricultural Depression.’ Glasgow, the economic capital of the Region, underwent phenomenal growth during the nineteenth century, and had a massive impact upon local agriculture. Glasgow and its satellite towns were a market for agricultural produce, and a source of imported livestock feed, and fertilisers. The fashions, in the town, for consumer goods and non-traditional foodstuffs spread out to the surrounding Region, and interaction between town and country was facilitated by the development of the railways. The significance of farm location in relation to Glasgow is assessed.

Au-delà des variables climatiques, d’autres facteurs non climatiques sont à considérer dans l’analyse de la vulnérabilité et de l’adaptation au changement et variabilité climatiques. Cette mutation de paradigme place l’agent humain au centre du processus d’adaptation au changement climatique, notamment en ce qui concerne le rôle des réseaux sociaux dans la transmission des nouvelles idées. Dans le domaine de l’agriculture, le recours aux innovations est prôné comme stratégie d’adaptation. L’élaboration et l’appropriation de ces stratégies d’adaptation peuvent être considérées comme des processus d’innovation qui dépendent autant du contexte social et culturel d’un territoire, de sa dynamique, ainsi que de la stratégie elle-même. Aussi, l’appropriation et la diffusion d’une innovation s’opèrent à partir d’un processus décisionnel à l’échelle de l’exploitation agricole, qui à son tour, demande une compréhension des multiples forces et facteurs externes et internes à l’exploitation et les multiples objectifs de l’exploitant. Ainsi, la compréhension de l’environnement décisionnel de l’exploitant agricole à l’échelle de la ferme est vitale, car elle est un préalable incontournable au succès et à la durabilité de toute politique d’adaptation de l’agriculture. Or, dans un secteur comme l’agriculture, il est reconnu que les réseaux sociaux par exemple, jouent un rôle crucial dans l’adaptation notamment, par le truchement de la diffusion des innovations. Aussi, l’objectif de cette recherche est d’analyser comment les exploitants agricoles s’approprient et conçoivent les stratégies d’adaptation au changement et à la variabilité climatiques dans une perspective de diffusion des innovations. Cette étude a été menée en Montérégie-Ouest, région du sud-ouest du Québec, connue pour être l’une des plus importantes régions agricoles du Québec, en raison des facteurs climatiques et édaphiques favorables. Cinquante-deux entrevues ont été conduites auprès de différents intervenants à l’agriculture aux niveaux local et régional. L’approche grounded theory est utilisée pour analyser, et explorer les contours de l’environnement décisionnel des exploitants agricoles relativement à l’utilisation des innovations comme stratégie d’adaptation. Les résultats montrent que les innovations ne sont pas implicitement conçues pour faire face aux changements et à la variabilité climatiques même si l’évolution du climat influence leur émergence, la décision d’innover étant largement déterminée par des considérations économiques. D’autre part, l‘étude montre aussi une faiblesse du capital sociale au sein des exploitants agricoles liée à l’influence prépondérante exercée par le secteur privé, principal fournisseur de matériels et intrants agricoles. L’influence du secteur privé se traduit par la domination des considérations économiques sur les préoccupations écologiques et la tentation du profit à court terme de la part des exploitants agricoles, ce qui pose la problématique de la soutenabilité des interventions en matière d’adaptation de l’agriculture québécoise. L’étude fait ressortir aussi la complémentarité entre les réseaux sociaux informels et les structures formelles de soutien à l’adaptation, de même que la nécessité d’établir des partenariats. De plus, l’étude place l’adaptation de l’agriculture québécoise dans une perspective d’adaptation privée dont la réussite repose sur une « socialisation » des innovations, laquelle devrait conduire à l’émergence de processus institutionnels formels et informels. La mise en place de ce type de partenariat peut grandement contribuer à améliorer le processus d’adaptation à l’échelle locale. / Other than climatic variables, non-climatic factors should be considered in the analysis of vulnerability and adaptation to climate change and variability. This shift in paradigm places the human agent at the centre of the process of adaptation to climate change, particularly with regard to the role of social networks in the transmission of new ideas. In agriculture, the use of innovations is advocated as a coping strategy. The development and adoption of these coping strategies can be considered innovative processes that depend as much on the social and cultural context of a country, its dynamics, and the strategy itself. Also, the ownership and dissemination of an innovation are taking place from a decision-making across the farm, which in turn requires an understanding of the multiple forces and external and internal factors in operation and the multiple objectives of the operator. Thus, understanding of the farmer’s decision- making environment at the farm level is vital because it is a prerequisite for the success and sustainability of any agricultural adaptation policy. However, in a sector like agriculture, it is recognized that social networks for example, play a crucial role in adaptation in particular, through the diffusion of innovations. Therefore, the objective of this research is to analyze how farmers take ownership and design strategies to adapt to climate change and variability from the perspective of diffusion of innovations. This study was conducted in Montérégie- West, a region located in the southwestern part of Quebec and which is known to be one of its most important agricultural regions, due to favorable climatic and soil factors. Fifty-two interviews were conducted with various stakeholders in agriculture at local as well as regional levels. The grounded theory approach is used to analyze and explore the contours of farmers’s decision-making environment regarding the use of innovation as a coping strategy. The results show that innovations are not implicitly designed to cope with climate change and variability even if climate change affects their emergence. The decision to innovate is largely determined by economic considerations. Moreover, the study also shows a weakness of social capital within farmers groups related to the overriding influence of the private sector, which are the main supplier of materials and agricultural inputs. The influence of the private sector has resulted in the dominance of economic considerations over environmental concerns and the temptation of short-term profit from the farmers, which raises the issue of sustainability of interventions in adaptation of Quebec’s agriculture. The study also highlights the complementarity between informal social networks and formal structures of support for adaptation, as well as the need to build partnerships. In addition, the study places the adaptation of Quebec’s agriculture from the perspective of private adaptation whose success is based on a "socialization" of innovations, which should lead to the emergence of formal and informal institutional processes. The establishment of such partnerships can greatly help improve the adaptation process at the local level.

Master of Science / Department of Agronomy / Ganga M. Hettiarachchi / Phosphorus (P) management in reduced tillage systems has been a great concern for farmers. Conclusive results for benefits of deep banding of P fertilizers for plant yield in reduced tillage system are still lacking. Knowledge of the dominant solid P species present in soil following application of P fertilizers and linking that to potential P availability would help us to design better P management practices. The objectives of this research were to understand the influence of placement (broadcast- vs. deep band-P or deep placed-P), fertilizer source (granular- versus liquid-P), and time on reaction products of P. Greenhouse and field based experiments were conducted to study P behavior in soils. Soil pH, resin extractable P, total P, and speciation of P were determined at different distances from the point of fertilizer application at 5 weeks (greenhouse and field) and 6 months (field) after P application (at rate 75 kg/ha) to a soil system that was under long-term reduced tillage. X-ray absorption near edge structure spectroscopy technique was used to speciate reaction products of fertilizer P in the soil. The reaction products of P formed upon addition of P fertilizers to soils were found to be influenced by soil pH, P placement methods, and P sources. Acidic pH (below~5.8) tended to favor formation of Fe-P and Al-P like forms whereas slightly acidic near neutral pH soils favored formation of Ca-P like forms. Scanning electron microscope with energy dispersive X-ray analysis of applied fertilizer granules at 5-wk showed enrichment of Al, Fe and Ca in granule- indicating these elements begin to react with applied P even before granules dissolve completely. The availability of an applied P fertilizer was found to be enhanced as a result of the deep banding as compared to the surface broadcasting or deep placed methods. Deep banded liquid MAP was found to be in more adsorbed P like forms and resulted greater resin extractable P both at 5 wk and 6 month after application. Deep banding of liquid MAP would most likely result both agronomically and environmentally efficient solution for no-till farmers.

Doctor of Philosophy / Agronomy / Allan K. Fritz / Bikram S. Gill / Gary M. Paulsen / Post-anthesis high temperature stress in wheat (Triticum aestivum L.) is a major cause of yield reduction. This process results in the loss of viable leaf area and a decrease in green leaf duration ultimately causing a yield loss. The objectives of this study were to (i) phenotype a recombinant inbred line population for heat tolerance traits, (ii) understand the genetic basis of heat tolerance by mapping quantitative trait loci (QTL) linked to yield-related traits under high temperature, (iii) model stay-green under high temperature stress and map the QTL linked to stay-green parameters, and (iv) validate the markers linked to QTL under field conditions. A filial6:7 (F6:7) recombinant inbred line (RIL) population was developed by crossing Ventnor, a heat-tolerant white winter wheat with Karl 92, a relatively heat susceptible hard red winter wheat. From 10 DAA to maturity, the treatments of optimum temperature or high temperature stress (30/25°C) were imposed on the RILs. The traits measured included grain filling duration (GFD), kernels per spike, thousand kernel weight (TKW), and grain filling rate (GFR). The stay-green traits calculated were: i) time between 75% and 25% green, ii) maximum rate of senescence, iii) time to maximum rate of senescence, and v) percent green at maximum senescence. Genetic characterization was performed using microsatellite (SSR), amplified fragment length polymorphism (AFLP) and a sequence tag site (STS) markers. GFD was positively correlated with TKW and negatively with GFR and maximum rate of senescence. Principle component analysis (PCA) showed kernels per spike, maximum rate of senescence, and TKW accounted for 98% of total variability among the genotypes for heat tolerance. The most significant QTL for yield traits co-localized with marker Xgwm296 for TKW, Xgwm356 for kernels per spike, and Xksum61 for GFR. The QTL for stay-green traits co-localized with markers P41/M62-107 on Chromosome 2A, Xbarc136 on Chromosome 2D, P58/MC84-146 on Chromosome 3B, P58/M77-343 on Chromosome 6A, and. P58/MC84-406 on Chromosome 6B. These results indicate that increased green leaf area duration has a positive effect on the grain yield under high temperature. Once the kernels per spike are established, GFD and TKW can be used as selection criteria for post-anthesis heat-tolerance.

Doctor of Philosophy / Department of Grain Science and Industry / Finlay I. MacRitchie / Expansion of dough and hence breadmaking performance is postulated to depend on a dual mechanism for stabilization of inflating gas bubbles. Two flours were used in this study, one from the wheat variety Jagger (Jagger) and the other from a composite of soft wheat varieties (soft). The primary stabilizing mechanism is due to the gluten-starch matrix surrounding the bubble. The secondary mechanism operates when gas bubbles come into close contact during later proofing and early baking. When discontinuities occur in the gluten-starch matrix surrounding gas bubbles, thin liquid lamellae stabilized by adsorbed surface active compounds, provide a secondary stabilization. A key parameter in the primary stabilizing dough film is thought to be the property of strain hardening. Jagger flour gave higher test-bake loaf volume than soft wheat flour and higher strain hardening index for dough. Rheological properties of doughs were varied by addition of protein fractions prepared by pH fractionation. Fractions were characterized by SE-HPLC and MALLS. The molecular weight distribution (MWD) of fractions progressively shifted to higher values as the pH of fractionations decreased. Mixograph peak development time paralleled the MWD. However, the strain hardening index and the test-bake loaf volume increased with increasing MWD up to a point (optimum), after which they declined. At a given strain rate the behavior at the optimum appeared to result from slippage of the maximum number of statistical segments between entanglements, without disrupting the entangled network of polymeric proteins. Shift of MWD to MW higher than the optimum results in a stronger network with reduced slippage through entanglement nodes, whereas a shift to lower MWs will decrease the strength of the network due to less number of entanglements per chain. In order to study the secondary stabilizing mechanism, different lipid fractions were added incrementally to the defatted flours. No effects were observed on the rheological properties of the dough. However, large effects on the loaf volume were measured. The additives used were the total flour lipid and its polar and non polar fractions and the fatty acids palmitic, linoleic and myristic. Polar lipids and palmitic acid had positive or little effect on loaf volume respectively. Non polar lipid, linoleic and myristic acids had negative effects on loaf volume. 1 The different effects of the lipid fractions are thought to be related to the type of monolayer that is formed. Polar lipid and palmitic acid form condensed monolayers at the air/water interface whereas non polar lipid, linoleic and myristic acids form expanded monolayers.

Doctor of Philosophy / Department of Horticulture, Forestry, and Recreation Resources / Candice A. Shoemaker / The objectives of this study were to determine exercise intensity of common gardening tasks in older adults and to investigate if older gardeners meet the physical activity (PA) recommendations (intensity and time) through their daily gardening. Kinds of gardening tasks, body postures, and bodily pain while gardening of older gardeners were investigated and the possibility of gardening as a predictor for a physically active lifestyle and life satisfaction in older adults was determined. Older participants were randomly recruited from the community of Manhattan, KS. To determine the exercise intensity of gardening, the heart rates of older adults were measured by radiotelemetry during gardening or garden tasks, and then oxygen uptake and energy expenditure were measured via indirect calorimetry using a submaximal graded exercise test. Overall health conditions by the Short Form 36 Health Survey (SF-36), hand functions by hydraulic hand dynamometer and pinch gauge, and bone mineral density (BMD) by dual-energy x-ray absorptiometry were measured. An observational study and weekly logs were conducted to study kinds of gardening, postures, and bodily pain of older gardeners. The Community Healthy Activities Model Program for Senior (CHAMPS) questionnaire was used to measure leisure-time PAs (frequency per week of all PAs and calories expended per week in all PAs). In conclusion, the nine gardening tasks were found to be low to moderate intensity PA in healthy older adults (1.6 ± 3.6 METs). Gardening observed was moderate intensity (3.8 ± 1.4 METs) PA in older adults and the subjects met the PA recommendation, which is at least 30 minutes of moderate intensity PA on most days of the week through their daily gardening (moderate intensity; average 33 hrs/wk in May and 15 hrs/wk in June and July). The older gardeners showed higher values for hand function and some SF-36 domains (physical function, bodily pain, and physical summary) than older non-gardeners. Gripping, stooping, lifting, stretching, walking, standing, kneeling, sitting, and squatting were observed while older adults gardened and lower back pain was the main bodily pain reported. Furthermore, gardening was found to be a predictor for leading a physically active lifestyle and high life satisfaction in older adults.

No description available.