The issue of fixed anchors in wilderness challenges modern interpretations of the Wilderness Act. The Wilderness Act can be interpreted to allow or preclude the placement of fixed anchors in designated wilderness areas depending on whether the interpreter chooses a literal or general interpretation of the law's meaning. Place-based management options, supported by descriptive and evaluative study results, may be more closely aligned to Wilderness Act directives than nationwide, blanket policy. This research is focused on understanding the role of fixed anchors in designated wilderness in order to develop management options that protect wilderness values without hindering wilderness users. The unique nature of each wilderness area and climbing resource, in combination with distinct preferences and motivations of different climber populations, lends itself to managing fixed anchors through place-based policy that considers the landscape and the preferences of individual visitors.Joshua Tree National Park [JTNP] was chosen as the location for this case study because it has committed to establishing fixed anchor management that accommodates rock climbing and protects wilderness resources. JTNP has prohibited the placement of fixed anchors in wilderness since February 1993. By combining resource inventories, spatial modeling, and wilderness climber profiles, study results reveal that wilderness visitor destination choice is not dependent on the location of fixed anchors, but the quality, difficulty, and distance to destinations. These results can be used as the basis for a wilderness fixed anchor permit system and demonstrate that fixed anchors can fit within the confines of the Wilderness Act. Place-based management options, relative to national policies, can minimize administrative burdens and limit the geographic extent of unintended regulatory effects. The Wilderness Act's minimum administrative requirement mandate promotes this type of management style. This study of the benign fixed anchor at JTNP illustrates the power of the scientific method to neutralize wilderness recreation conflict and support streamlined wilderness management, while at the same time highlighting the shortcomings, and strengths, of the Wilderness Act as it is applied to a new era of wilderness recreation management.

Water resource infrastructure in cold regions of the world can be significantly impacted by the existence of river ice. Major engineering concerns related to river ice include ice jam flooding, the design and operation of hydropower facilities and other hydraulic structures, water supplies, as well as ecological, environmental, and morphological effects. The use of numerical simulation models has been identified as one of the most efficient means by which river ice processes can be studied and the effects of river ice be evaluated. The continued advancement of these simulation models will help to develop new theories and evaluate potential mitigation alternatives for these ice issues. In this thesis, a literature review of existing river ice numerical models, of anchor ice formation and modeling studies, and of aufeis formation and modeling studies is conducted. A high level summary of the two-dimensional CRISSP numerical model is presented as well as the developed freeze-up model with a focus specifically on the anchor ice and aufeis growth processes. This model includes development in the detailed heat transfer calculations, an improved surface ice mass exchange model which includes the rapids entrainment process, and an improved dry bed treatment model along with the expanded anchor ice and aufeis growth model. The developed sub-models are tested in an ideal channel setting as somewhat of a model confirmation. A case study of significant anchor ice and aufeis growth on the Nelson River in northern Manitoba, Canada, will be the primary field test case for the anchor ice and aufeis model. A second case study on the same river will be used to evaluate the surface ice components of the model in a field setting. The results from these cases studies will be used to highlight the capabilities and deficiencies in the numerical model and to identify areas of further research and model development.

Water resource infrastructure in cold regions of the world can be significantly impacted by the existence of river ice. Major engineering concerns related to river ice include ice jam flooding, the design and operation of hydropower facilities and other hydraulic structures, water supplies, as well as ecological, environmental, and morphological effects. The use of numerical simulation models has been identified as one of the most efficient means by which river ice processes can be studied and the effects of river ice be evaluated. The continued advancement of these simulation models will help to develop new theories and evaluate potential mitigation alternatives for these ice issues. In this thesis, a literature review of existing river ice numerical models, of anchor ice formation and modeling studies, and of aufeis formation and modeling studies is conducted. A high level summary of the two-dimensional CRISSP numerical model is presented as well as the developed freeze-up model with a focus specifically on the anchor ice and aufeis growth processes. This model includes development in the detailed heat transfer calculations, an improved surface ice mass exchange model which includes the rapids entrainment process, and an improved dry bed treatment model along with the expanded anchor ice and aufeis growth model. The developed sub-models are tested in an ideal channel setting as somewhat of a model confirmation. A case study of significant anchor ice and aufeis growth on the Nelson River in northern Manitoba, Canada, will be the primary field test case for the anchor ice and aufeis model. A second case study on the same river will be used to evaluate the surface ice components of the model in a field setting. The results from these cases studies will be used to highlight the capabilities and deficiencies in the numerical model and to identify areas of further research and model development.

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The initial focus of this PhD project was on comparative gene mapping. Comparative gene mapping is facilitated by consensus PCR primers which amplify homologous gene fragments in many species. As a part of an international co-ordinated programme of comparative mapping in pigs, 47 CATS (Comparative Anchor Tagged Sequence) consensus primer pairs for loci located on human chromosomes 9, 10, 20, and 22, were used for amplifying homologous loci in pigs. After optimization of PCR conditions, 23 CATS products have confirmed by comparison with homologous sequences in GenBank. A French somatic cell hybrid panel was used to physically map the 6 porcine CATS products distinguishable from rodent background product, namely ADRA1A, ADRA2A, ARSA, GNAS1, OXT and TOP1. Of these, the map location of ADRA1A and OXT showed inconsistency with the previously recognised conserved relationship between human and pig. The other four loci mapped to positions consistent with known syntenic relationships. Despite low levels of polymorphism, frequently indistinguishable rodent and porcine products in somatic hybrids and some confusion of identity of gene family members, these CATS primers have made a useful contribution to the porcine-human comparative map. The focus of the project then changed to genetic and molecular characterisation of endogenous retroviruses in pigs and their relatives. Pigs are regarded as a potentially good source of organs and tissues for transplantation into humans. However, porcine endogenous retroviruses have emerged as a possible problem as they can infect cultured human cells. Two main types of pig retrovirus, determined by envelope protein, PERV-A and PERV-B, are widely distributed in different pig breeds and a third less common type, PERV-C, has also been recognised. Endogenous retroviruses were analyzed from the Westran (Westmead transplantation) inbred line of pig, specially bred for biomedical research. Thirty-one 1.8 kb env PCR product clones were sequenced after preliminary screening with the restriction enzymes KpnI and MboI. Five recombinant clones between A and B were identified. 55% of clones (17/31) sequenced had stop codons within the envelope protein-encoding region, which would prevent the retrovirus from making full-length envelope protein recognizable by cell-surface receptors of the virus. The endogenous viruses were physically mapped in Westran pigs by FISH (Fluorescence In Situ Hybridisation) using PERV-A and PERV-B envelope clones as probes. Preliminary FISH data suggest that there are at least 22 PERVs (13 PERV-A and 9 PERV-B) and the chromosomal locations of these in the Westran strain are quite different from European Large White pigs. The sequences and mapping results of inbred Westran pig suggest that there are relatively few PERV integration sites compared with commercial pigs and further that a large proportion of clones are defective due to premature stop codons in the envelope gene. To investigate the relationship of endogenous retroviruses in peccaries and pigs, a set of degenerate primers was used to amplify peccary retroviral sequences. The sequences of two putative retroviral clones showed close homology, albeit with a 534 bp deletion, to mouse and pig retroviral sequences. Also, four non-target sequences were amplified from peccary with the degenerate retroviral primers. They are a part of the peccary cofilin gene, a SINE, and a sequence containing a microsatellite. The peccary endogenous retroviral sequences are significant in that they are the first such sequences reported in peccary species and repudiate old claims in the literature that peccaries do not have C-type retroviral sequences.

Thesis (Ph.D. in Pharmacology) -- University of Colorado Denver, 2008. / Typescript. Includes bibliographical references (leaves 135-162). Free to UCD affiliates. Online version available via ProQuest Digital Dissertations;

Orientador: Eliane Mauerberg de Castro / Banca: Cynthia Yukiko Hiraga / Banca: Renato de Moraes / Resumo: O objetivo deste trabalho é avaliar, ao longo de uma caminhada, a contribuição do sistema háptico durante a condução de um cão em condições de desafios ao sistema postural. Os desafios nas tarefas deste estudo incluem restrição da base de suporte para a marcha e privação visual. Esperamos que a sincronização entre condutor e cão se reflita em melhores níveis de desempenho no controle da marcha, especialmente na condição onde a visão é obstruída. Para este estudo foram recrutados 10 adultos jovens, com idade de média de 23,7 ± 4,32 anos e um cão treinado em obediência básica e na tarefa de condução em superfície restrita de apoio (traves de equilíbrio). As condições de tarefas foram: com e sem restrição da visão (CVSC e SVSC, respectivamente), e com e sem condução do cão (CVCC e SVCC, respectivamente). A primeira condição foi sempre a condição controle (olhos abertos sem condução do cão). Cada tentativa foi repetida três vezes. As tarefas foram filmadas por duas câmeras com o objetivo de avaliar a duração de cada ciclo do andar. Uma ANOVA three-way com medidas repetidas em todos os fatores (visão, tarefa e repetições) foi realizada para as variáveis: duração do ciclo de cada passada, média da fase relativa e o desvio da fase relativa, com medidas também repetitivas em todos os fatores para: visão, tarefa, pernas, e repetições. Testes a posteriori de Bonferroni foram calculados para comparações aos pares. Em relação à média da fase relativa as tarefas executadas com o cão foi significativamente superior àquelas sem o uso do cão. O pior relacionamento temporal foi mantido nas médias... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The purpose of this study was to assess the contribution of the haptic system during walking with a dog on challenging tasks to the postural system. The challenges included restriction to the base support (walking on a balance beam for both, the human and the dog), and visual occlusion (for the human only). We expected that the synchronization between the walking individual and the dog reflected in improved levels of performance in motion control, especially in conditions where vision is occluded. We recruited 10 young adults with average age of 23.7 ± 4.32 years and a dog with obedience training and conditioned to walk on a restricted area of support (balance beam). The task conditions were: with and without vision (CVSC and SVSC, respectively), and with and without conducting the dog (CVCC and SVCC, respectively). The first condition was always the control condition (eyes open without conducting the dog). Each trial was repeated three times. The tasks were videotaped with two cameras in order to process the duration of each stepping cycle. A three-way ANOVA with repeated measures on all factors (vision, and task repetitions) was performed for the variables: duration of each stepping cycle, relative phase and standard deviation of the relative phase, also with repeated measures on all of the factors: vision, task conditions, legs, and trials. The mean relative phase of the tasks performed with the dog was significantly closer to symmetry than those without the use of the dog. The worst temporal relationship was maintained during the condition SVSC (right leg = 0,477 ± 0,030, left leg = 0,544 ± 0,035). However... (Complete abstract click electronic access below) / Mestre

A técnica da fotoelasticidade pode ser particularmente útil na análise de tensões em sistemas com geometrias complexas. Em um equipamento chamado polariscópio, um feixe de luz polarizada atravessa o modelo fotoelástico que representa o protótipo. Ao submeter este modelo a um carregamento, faixas de diferentes cores poderão ser visualizadas. Cada cor é associada a um nível de tensão. As tensões observadas no modelo fotoelástico podem ser comparadas com as tensões determinadas nos modelos numéricos ou analíticos. Em casos específicos, o problema da distribuição de tensões em uma fundação pode ser analisado com emprego da fotoelasticidade. A análise do comportamento de estacas helicoidais através da técnica da fotoelasticidade pode ser então de grande valia para estudos mais aprofundados deste sistema. O principal objetivo desta pesquisa foi viabilizar este método experimental para ser aplicado no Departamento de Geotecnia da EESC em análises de tensões em sistemas geotécnicos. Para validar as análises experimentais, tensões observadas em modelos de geometria simples foram comparadas com as tensões determinadas por meio de modelagem numérica com o método dos elementos finitos. O comportamento da fundação por estaca helicoidal observado pela técnica da fotoelasticidade mostrou semelhança ao observado na modelagem numérica. Por meio da distribuição de tensões, foi possível avaliar a influência da geometria helicoidal da lâmina e da profundidade de instalação da ancoragem no terreno. / The photoelastic method can be particularly useful for stress analysis with complex geometry systems. In equipment called polariscope, a polarized light beam crosses the photoelastic model that represents the prototype. Submitting this model to a load, fringes of different colors can be visualized. Each color is associated to a stress level. The stresses corresponding to the observed fringes on the photoelastic model can be compared to the stresses obtained through analytical or numerical models. In specific cases, the stress distribution problem can be analyzed with the use of photoelasticity. The photoelastic analysis of the helical pile behavior can then be valuable for further studies of this system. The main objective of this research was enabling this experimental method to be applied in the Department of Geotechnical Engineering of EESC in geotechnical systems stress analysis. To validate the experimental analysis, stresses observed in simple geometry models have been compared to those stresses determined by numerical modeling with the finite element method. The observed behavior of the helical pile foundation by photoelastic method showed similarity to that one observed through numerical modeling. Stress distribution analyses allowed evaluating the influence of helical blade geometry and the depth of installation.

Atualmente a área de saúde vem buscando auxílio na engenharia para a contribuição no estudo e confecção de próteses para casos específicos de fraturas ou doenças ósseas. Com o avanço da tecnologia surgiram processos de manufatura que tornam possível a fabricação de próteses personalizadas. Uma dessas tecnologias é a manufatura aditiva. O grande desafio no momento está na fabricação de próteses por manufatura aditiva que combinem desempenho biomecânico e resistência estrutural. Nesse contexto, o objetivo deste trabalho é desenvolver elementos de fixação, especificamente âncoras de sutura de polímeros PEEK e PEKK, considerando seus requisitos estruturais através de simulações pelo método dos elementos finitos, e considerando que sejam fabricadas através de processos de manufatura aditiva. Este desenvolvimento permitirá a substituição de âncoras de sutura de liga de titânio, hoje largamente empregadas pelos cirurgiões, por âncoras de sutura de polímeros biocompatíveis, o que possibilitará que estes polímeros sejam absorvidos pelo corpo humano em curto e médio prazo, permitindo a completa restituição do osso afetado, melhorando a qualidade de vida do paciente pós-cirurgia. Os resultados obtidos neste trabalho indicam que a técnica poderá resultar em próteses com a biocompatibilidade desejada e resistência mecânica adequada. Próteses permanentes (articulações) ou provisórias (âncoras de sutura) seriam um dos critérios para a escolha do material a ser utilizado, absorvível ou não pelo corpo humano. / Nowadays medicine is searching assistance from engineering that may contribute in studies and confection of prothesis to specific cases of fractures and bone diseases. With the advance of technology new processes of manufacture were risen, making possible the confection of personalized prothesis. The biggest challenge of the moment is in the manufacture of additive prothesis that can combine biomechanics performance and structural resistance. In this context, the objective of this dissertation is to develop PEEK and PEKK polymer suture anchors, considering its structural requirements, taking into account their mechanical strength, through simulation by the finite element method, and considering additive manufacturing processes. This development will allow the replacement of titanium alloy suture anchors, now widely used by surgeons by suture anchors of biocompatible polymers, which enable these polymers to be absorbed by the human body in the short and medium term, allowing full regeneration of affected bone, improving the quality of life of the patient. The results of this study indicate that the technique could result in prosthesis with the desired biocompatibility and adequate mechanical strength. Permanent prosthesis (joints) or temporary (suture anchors) would be one of the criteria for the choice of material to be used, absorbable or non absorbable by the human body.

O aumento no número de idosos nos últimos anos trouxe impactos sociais e econômicos. O processo de envelhecimento e/ou patologias específicas trazem consigo perdas progressivas sensoriais, motoras e de processamento central que estão relacionadas com o aumento das quedas com o avanço da idade. Estas perdas predispõem o idoso a sofrer com limitações físicas de mobilidade, medo de cair ou mesmo sofrer com quedas. Este quadro evoca a necessidade de atenção especial à qualidade de vida dos idosos, com novas intervenções e programas de reabilitação física. Dentre essas novas abordagens para melhorar o funcionamento do controle postural, temos a adição de informação háptica que contribui para reduzir a oscilação corporal durante a manutenção da postura ereta. Uma das formas de adicionar informação háptica ao sistema de controle postural é pelo sistema âncora. Este é uma ferramenta não rígida que consiste de dois cabos maleáveis com 125g de massa repousando no solo e com a outra extremidade segura pelas mãos (âncora tradicional). Considerando que o toque leve em diferentes partes do corpo reduz a oscilação corporal, é possível, da mesma forma, que o uso do sistema âncora em diferentes partes do corpo seja efetivo. Portanto, o objetivo desse estudo foi avaliar o efeito do uso do sistema âncora em diferentes pontos de contato (antebraço e ombro) sobre a oscilação corporal de idosos durante a manutenção da postura ereta Trinta idosos participaram do presente estudo. Eles permaneceram na postura ereta com os pés na posição semi tandem sobre uma plataforma de força para a obtenção do deslocamento do centro de pressão (CP). Cinco condições experimentais foram realizadas: sem âncora, âncora tradicional, âncoras nas mãos fixadas com presilhas, âncoras nos antebraços fixadas com presilhas e âncoras nos ombros fixadas com presilhas. Baseada nos dados fornecidos pelo deslocamento do CP foram calculadas as variáveis: área da elipse, amplitude média de oscilação e velocidade média de oscilação. Os resultados mostraram uma redução da área da elipse e da amplitude média de oscilação na direção anteroposterior nas condições com as âncoras posicionadas nas mãos (tradicional e presilha) e antebraços em comparação a condição sem âncora. Os resultados apontaram ainda uma redução da velocidade média de oscilação na direção anteroposterior na condição de âncoras nos antebraços em comparação a condição sem âncora. Na direção mediolateral, a velocidade média de oscilação reduziu nas condições de âncora fixada nas mãos e antebraços em comparação a condição sem âncora. O uso do sistema âncora na região dos ombros não trouxe redução da oscilação corporal. Em conclusão, o sistema âncora reduziu a oscilação corporal quando o sistema âncora foi posicionado nos antebraços da mesma forma que quando foi fixado/seguro nas mãos. A melhora observada com o uso das âncoras parece não estar relacionada com a quantidade de receptores táteis no ponto de contato dos cabos das âncoras / The increase in the number of older adults in recent years has brought social and economic impacts. The process of aging and/or specific diseases carries progressive sensory, motor and central processing losses that are related to the increased rate of falls with increasing age. These losses predispose the elderly to suffer from physical mobility limitations, fear of falling or even falls. This scenario evokes the need for special attention to the quality of life for older adults, with new interventions and physical rehabilitation programs. Among these new approaches to improve the functioning of the postural control system, we have the addition of haptic information which helps to reduce body sway during maintenance of upright posture. One way to add haptic information to the postural control system is the anchor system. It consists of a non-rigid tool with two flexible cables with 125g mass resting on the ground and with the other end secured by the hands (traditional anchor). Since light touch in different body regions reduces body sway, it is possible, likewise, that the use of the anchor system in different parts of the body to be effective in reducing body sway. Therefore, the aim of this study was to evaluate the effect of using the anchor system in different points of contact (forearm and shoulder) on body sway of older adults during maintenance of upright posture Thirty older adults participated in this study. They stood upright on a force plate to obtain center of pressure displacement (CoP) with their feet in the semi tandem position. Five experimental conditions were carried out: no anchor, traditional anchor, anchors on the hands secured with clasps, anchors on the forearms secured with clasps and anchors on shoulders secured with clasps. Based on data provided by the CP displacement, the following variables were calculated: ellipse area, mean sway amplitude and mean sway velocity. The results showed a reduction in the area of the ellipse and in the mean sway amplitude (anteriorposterior direction) for conditions with anchors hold on the hands and forearms compared to the no anchor condition. The results also indicated a reduction in the mean sway velocity (anterior-posterior direction) with the anchors on the forearms compared to the no anchor condition. For the medial-lateral direction, the mean sway velocity reduced with the anchor on the hands and forearms compared to the no anchor condition. The use of the anchor system in the shoulder region did not reduce body sway. In conclusion, the use of the anchor system reduced body sway when it was placed on the forearms in the same way as when placed/secured on the hands. The improvement observed with the use of anchors does not seem to be related to the amount of tactile receptors in the point of contact of the anchor cables