A imunoglobulina E (IgE) tem papel central na patogênese das doenças alérgicas. É parte da resposta do tipo 2, e as citocinas IL-4 e IL-13 são essenciais para que haja produção deste isotipo de imunoglobulina. Produção de IgE é também induzida por parasitas intestinais, particularmente helmintos. O objetivo do presente estudo foi avaliar a contribuição de IgE específica para ácaros, barata, gato, cachorro e para o parasita Ascaris lumbricoides sobre os níveis de IgE total entre crianças de áreas distintas no Brasil. Anticorpos IgE para ácaros Dermatophagoides pteronyssinus e Blomia tropicalis; barata Blattella germanica; gato; cachorro; e A. lumbricoides foram medidos usando o sistema ImmunoCAP, e comparados à IgE total no soro de 150 crianças de 3-6 anos de idade vivendo na cidade de Natal, endêmica para parasitoses intestinais, e de 54 crianças de 3-6 anos de idade com asma e/ou rinite, vivendo em Ribeirão Preto. Níveis de IgE total foram significantemente mais elevados em crianças de Natal quando comparados aqueles em crianças de Ribeirão Preto (média geométrica 630,9 kU/L, faixa 19,6-63.290 kU/L; e 398,1 kU/L, faixa 35,7-4.803 kU/L, respectivamente). Entre as 150 crianças de Natal, 52(34,6%) apresentaram IgE positiva para D.pteronyssinus; 70(46,6%) para B.tropicalis; 45(30%) para barata; 19(12,6%) para gato; 17(11,3%) para cachorro; e 125(83,3%) para A.lumbricoides. Entre as 54 crianças com asma e/ou rinite de Ribeirão Preto, 41(75,9%) apresentaram IgE positiva para D.pteronyssinus; 34(62,9%) para B.tropicalis; 22(40,7%) para barata; 11(20,3%) para gato; 12(22,2%) para cachorro. Embora estas crianças fossem negativas para parasitas à inclusão no estudo, 22(40,7%) tinham IgE para A.lumbricoides. Anticorpos IgE para A. lumbricoides foram mais elevados entre crianças de Natal, quando comparados a IgE para alérgenos inalantes (p<0,01). Níveis de IgE para D. pteronyssinus entre crianças de Ribeirão Preto foram mais altos que IgE para outros inalantes e A.lumbricoides (p<0,01). Em Natal, a porcentagem de IgE para A. lumbricoides em relação à IgE total foi maior em comparação a IgE para D.pteronyssinus e B.germanica (mediana 0,41%; 0,08%; e 0,04% respectivamente, p<0,01). Em Ribeirão Preto, a porcentagem de IgE para D.pteronyssinus e para B.tropicalis em relação à IgE total foi maiorem comparação a IgE para A.lumbricoides e barata (mediana 9,8%; 0,6%; 0,3%; e 0,2%, respectivamente, p<0,05). Regressão linear revelou que a associação mais forte foi para IgE para A.lumbricoides com IgE total em Natal (R²=0,56; p<0,01); associação significante foi também observada para IgE para ácaros com IgE total em Ribeirão Preto (R2=0,35; p<0,01 para D.pteronyssinus; R2=0,33; p<0,01 para B.tropicalis, respectivamente). Nossos resultados demonstraram que anticorpos IgE para ácaros contribuem fortemente para a IgE total entre crianças com asma e/ou rinite, vivendo em uma área de baixa taxa de infecções parasitárias em nosso meio. Por outro lado, entre crianças vivendo em uma área em que parasitas são encontrados em abundância, infecções parasitárias induzem uma forte resposta IgE policlonal, e anticorpos IgE específicos para parasita, além de ácaros, barata, gato e cachorro representam uma modesta proporção da IgE total. A especificidade desta IgE, e os efeitos a longo prazo desta resposta cedo na vida, permanecem desconhecidos. / Immunoglobulin E (IgE) plays a central role in the pathogenesis of allergic diseases. It is part of the type 2 response, and the cytokines IL-4 and IL-13 are essential for the production of this immunoglobulin isotype. IgE production is also induced by intestinal parasites, particularly helminths. The aim of the present study was to evaluate the contribution of specific IgE to mites, cockroach, cat, dog and parasite Ascaris lumbricoides on total IgE levels among children living in different areas in Brazil. IgE antibodies to mites Dermatophagoides pteronyssinus and Blomia tropicalis; cockroach Blattella germanica; cat; dog; and A. lumbricoides were measured using the ImmunoCAP system and compared to total serum IgE of 150 children 3-6 year-old living in the city of Natal, endemic for intestinal parasites, and 54 children 3-6 years of age with asthma and /or rhinitis, living in Ribeirão Preto. Total IgE levels were significantly higher in children from Natal as compared to those among children in Ribeirão Preto (geometric mean 630,9 kU/L, range 19,6-63.290 kU/L; e 398,1 kU/L, range 35,7-4.803 kU/L, respectively). Among the 150 children from Natal, 52(34.6%) presented IgE positive to D.pteronyssinus; 70(46.6%) to B. tropicalis; 45(30%) to cockroach; 19 (12.6%) to cat; 17(11.3%) to dog; and 125(83.3%) to A. lumbricoides. Among the 54 children with asthma and /or rhinitis from Ribeirão Preto, 41(75.9%) had IgE positive to D.pteronyssinus; 34(62.9%) to B.tropicalis; 22(40.7%) to cockroach; 11(20.3%) to cat; 12(22.2%) to dog. Although these children were negative for parasites at inclusion in the study, 22(40.7%) had IgE to A.lumbricoides. IgE antibodies to A.lumbricoides were higher among children from Natal, as compared to IgE to inhalant allergens (p<0.01). IgE levels to D.pteronyssinus among children living in Ribeirão Preto were higher than IgE to other inhalants and A.lumbricoides (p<0.01). In Natal, the percentage of IgE to A.lumbricoides in relation to total IgE was higher in comparison to IgE to D.pteronyssinus e B.germanica (median 0.41%, 0.08%, and 0.04% respectively, p<0.01). In Ribeirão Preto, the percentage of IgE to D.pteronyssinus and to B.tropicalis in relation to total IgE was higher in comparison to IgE to A.lumbricoides and cockroach (median 9.8%, 0,6%, 0.3%, and 0.2%, respectively, p <0.05). Linear regression analysis revealed that the strongest association was for IgE to A.lumbricoides with total IgE in Natal (R²=0.56, p<0.01); significantassociation was also observed for IgE to mites with total IgE in Ribeirão Preto (R2=0.35, p<0.01 for D. pteronyssinus, R2=0.33, p<0.01 for B.tropicalis, respectively). Our results demonstrated that IgE antibodies to mites contribute strongly to total IgE among children with asthma and /or rhinitis, living in an area of low parasite infection rates in our country. On the contrary, among children living in an area where parasites are found in abundance, parasitic infections induce a strong polyclonal IgE response, and IgE antibodies specific for parasite, and also for mites, cockroaches, cat and dog represent a modest proportion of total IgE. The specificity of these IgE antibodies and the implications of this response occurring early in life remain unknown.

The reduction of pathogenic microorganisms is essential to minimize human health risks associated with the reuse, reclamation, and recycling of wastewater and the land application of biosolids (sewage sludge). The most advanced treatment technologies, as well as, the most representative methods and indicator organisms are necessary to ensure public safety. The goal of this dissertation is to assess advanced Bardenpho wastewater treatment technologies in regards to virus removal, suggest the most appropriate viral indicators of human fecal contamination and/or treatment process controls, and develop an updated method for enumerating Ascaris ova viability in land applied biosolids. Appendix A evaluates the incidence of 11 different virus types in sewage throughout a 12-month time period, and their subsequent reduction via advanced Bardenpho treatment processes. This study showed that wastewater treatment facilities utilizing advanced Bardenpho for secondary treatment are more effective at reducing viruses in wastewater than facilities utilizing conventional aeration basin and trickling filter processes. Appendix B develops a new method for determining the viability of Ascaris ova in land applied biosolids. In this method, early development stages prior to larval-development, are included in the estimation of potential viability. Comparisons between viability enumerations suggests that the conventional microscopy method, in which only ova containing motile larva are considered viable, underestimates the number of eggs that may progress to an infectious stage. Whereas, the method based on early-to-late stage development, considers the potential viability of all eggs, providing a more conservative approach.

Background: Soil transmitted helminth infections (STHI) are important Neglected Tropical Diseases (NTD). The three main STHI are infections with Ascaris lumbricoides, Trichuris trichiura and hookworms. STHI have a significant effect on the growth and development of children. A national survey for STHI in El Salvador by Pan American Health Organization and Ministry of Health in 2012 in school children aged 8years to 10 years. The survey collected data on age, gender, behavioral habits, and source of drinking water, type of toilet facility used and ecological zone of residence. A) We did an analyses of the data with an aim to determine the prevalence of STHI in El Salvador, assess the risk factors and risk interactions. B) We also aimed to determine the efficacy of urea as a potential additive for inactivation of Ascaris suum in solar toilets. Methods: A) Data from 1310 subjects was analysed for determination of prevalence of STHI in El Salvador. Risk factor assessment was done by chi-square test, unadjusted logistic regression and fully adjusted logistic regression. Risk factor interactions was tested on multiplicative and additive scale. B) Urea was tested for efficacy in inactivation of Ascaris suum ova in 20 solar toilets. Under conditions of controlled pH and moisture, concentration of gas ammonia, peak temperature were measured along with duration of treatment with urea to determine viability of Ascaris suum samples placed in the solar toilets. Results: I) The prevalence of Ascaris lumbricoides in 8-10 year old school children is 2.75%, Trichuris trichiura is 4.1% and hookworm is 1.83%. A) For Ascaris lumbricoides infection: Significant risk in individuals from volcanic chains and central depression compared to those from the mountains. Spring or well water when used as source of drinking water was associated with higher risk of infection when compared with piped water. Higher infection was also associated with open air defecation compared to use of septic tank or flush toilet. Use of sandals or no footwear was associated with a higher risk of infection when compared to use of closed footwear at all times. B) For Trichuris infection: Coastal plains were associated with a higher risk of infection compared to the mountains while rural status was protective against infection. Spring or well water when used as source of drinking water was associated with higher risk of infection when compared with piped water. Use of sandals or no footwear was associated with a higher risk of infection when compared to use of closed footwear at all times. C) For hookworm infection: Risk of infection was higher in individuals from urban regions. Spring or well water when used as source of drinking water was associated with higher risk of infection when compared with piped water. Use of sandals or no footwear was associated with a higher risk of infection when compared to use of closed footwear at all times. Poor handwashing was shown to be protective against infection with hookworm. Significant risk factor interactions were identified for infection with each of the three soil transmitted helminths. II) Urea as an additive at 1%w/w to feces tested in solar toilets showed an inactivation rate of nearly half the Ascaris suum ova samples. Fifty percent or higher inactivation rates were associated with ammonia gas concentrations of 109.5 ppm or higher and duration of treatment of 72 hours or higher. Conclusions: Prevalence of STHI in 8-10 year old school children for 2012 in El Salvador is low. Significant risk factors for STHI in El Salvador are eco-epidemiologic zone, source of drinking water, type of sanitation, use of shoes behavior and urban status of place of residence. Use of urea for inactivation of soil transmitted helminth ova in feces is a possible intervention for environmental control of STHI.

Ball State University LibrariesLibrary services and resources for knowledge buildingMasters ThesesThere is no abstract available for this thesis.

In this study, die-off patterns of helminth ova, fecal coliforms, and Salmonella spp. in biosolids were documented using three small-scale sand drying beds located in a greenhouse. Treatments involved tilling the biosolids with differing frequencies. The results indicate that the inactivation rate for helminth ova was 0.88, 0.55, and 0.22 eggs/4 g TS day-1 for the intensively-tilled, moderately-tilled, and control beds, respectively. Achievement of Class A criteria was only possible in the intensively-tilled bed by Day 70 of the experiment. Salmonella spp. were inactivated to Class A levels in 9 days for the intensively and moderately-tilled beds. Regrowth of Salmonella spp. occurred thereafter in all beds, but high levels were seen only in the control bed. Fecal coliforms reached Class A criteria late in the experiment. Tilling treatments enhanced the inactivation rate of helminth ova and offer a potentially cost-effective method of pathogen reduction.

No description available.

Monoclonal antibodies (MAbs) have been raised against $ beta$-tubulin of B. pahangi and A. suum. Anti-B. pahangi MAbs were used to investigate the heterogeneity of tubulins from nematodes and mammals. One-dimensional SDS-PAGE showed that MAbs P3D and 1B6 react with $ beta$-tubulin from a number of filarial and intestinal nematodes, but not with tubulin from protozoan and mammalian cells. Two-dimensional SDS-PAGE demonstrated that MAb P3D recognizes two isoforms of $ beta$-tubulin and 1B6 recognizes one. Limited proteolysis showed that MAb 1B6 reacted with the amino-terminal fragments and MAb P3D with the carboxyl-terminal fragments of $ beta$-tubulin. The effect of anti-B. pahangi MAbs on the viability of adult B. pahangi was assessed using MTT assay. It was found that MAbs P3D and 1B6 caused an 80% and 40% reduction respectively, in worm viability, whereas anti-chick MAb 357 or mebendazole drug had no effect. Immunogold labelling of B. pahangi demonstrated the presence of tubulin in the median and basal layers of the cuticle, hypodermal layer and somatic muscle blocks, as well as the uterus of B. pahangi. The reduction in the viability of worms may, therefore, be due to the disruption of microtubules in the body wall muscle of B. pahangi. The total MBZ binding was highest in the intestine followed by the body wall muscle and in the reproductive tract extracts of A. suum. Electron microscopy of A. suum tissues demonstrated that the tubulin content decreased from the intestine through the body wall muscle to the reproductive tract. One dimensional SDS-PAGE revealed the presence of $ alpha,$ $ beta sb1$ and $ beta sb2$ tubulin subunits in all tissues of A. suum. This data confirmed the reduction of tubulin from the intestine through the body wall muscle to the reproductive tract. Two dimensional SDS-PAGE followed by Western blotting demonstrated that $ alpha$ and $ beta$ tubulin isoform patterns are dissimilar in different tissues of A. suum. Body wall muscle, inte

Doutor -- Escola Nacional de Saude Publica, Rio de Janeiro, 2002.

PINTO, A. C. M. D. Efeito anti-inflamatório e imunomodulador de componentes de ascaris suum em artrite experimental, 2017.118 f.Tese (Doutorado em Ciências Médicas) - Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, 2017 / Submitted by Ivone Sousa (ppgcm.ufc@gmail.com) on 2017-07-21T13:27:56Z No. of bitstreams: 1 2017_tese_acmdpinto.pdf: 10927136 bytes, checksum: 4648aa6dab4dc5fb388eac8229d92bb9 (MD5) / Approved for entry into archive by Erika Fernandes (erikaleitefernandes@gmail.com) on 2017-07-21T15:51:59Z (GMT) No. of bitstreams: 1 2017_tese_acmdpinto.pdf: 10927136 bytes, checksum: 4648aa6dab4dc5fb388eac8229d92bb9 (MD5) / Made available in DSpace on 2017-07-21T15:51:59Z (GMT). No. of bitstreams: 1 2017_tese_acmdpinto.pdf: 10927136 bytes, checksum: 4648aa6dab4dc5fb388eac8229d92bb9 (MD5) Previous issue date: 2017-07-10 / Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease with multifactorial etiopathogenesis, involving genetic, immunological and environmental mechanisms, whose diagnosis and delayed treatment have a negative influence on the prevalence and severity of the disease. Therefore, patients residing in low-income countries should show increased morbidity and / or mortality from these diseases. Interestingly, the evolution seems to be similar, if not less severe, to that found in populations with better socioeconomic indexes. The hygienic hypothesis suggests an inverse relationship between the global distribution of autoimmune diseases and parasitic infections, where helminths tend to stimulate the development of Th2 responses and suppress Th-1 and Th-17 responses. The aim of this study was to isolate components in Ascaris suum extract and characterize its anti-inflammatory mechanism in experimental arthritis models. Ascaris suum crude extract was separated into fractions > 30 and <30 kDa. Four smaller fractions (1-4) were obtained, in the fraction <30 kDa, by native electrophoresis. In fraction 1, the ABA-1 protein was identified by mass spectrometry and after protein digestion with trypsin, seven peptides were isolated (Aba-AG). The genomic sequence of the Aba-B and Aba-F peptides was inserted into a commercial plasmid (VR2 -001-TOPO ™). Plasmids were cloned and expanded in E. coli and extraction of plasmid DNA was performed by commercial kit (Qiagen ™). Male Swiss mice received crude extract of A. suum (1mg), > 30kDa, <30kDa (50ng) or fractions 1, 2, 3 and 4 (1μL), diluted in sterile saline (200 μL), p.o., 30min before of the injection of Zy (0.1mg) i.a. Hypernociception was evaluated by the electronic von Frey and the cellular influx was evaluated in the joint fluid 6h or seven days after zymosan i.a. Swiss and Balb/c male mice, submitted to ZyA and AIA, respectively, received recombinant plasmids of Aba-B or Aba-F (50μg/30μL) at the back of each hind leg. The cell influx was evaluated 6h or seven days (ZyA) and 7h or 14 days (AIA) after the i.a stimulus. Cytokines were evaluated in the synovial fluid. Synovitis and joint damage were evaluated by H&E and safranin O, respectively, and mast cell by toluidine blue. Levels of cytokines (CXCL-1, IL-1β, IFN-γ, IL- 5, IL-6 and IL-10) were assessed by ELISA and the expression of iNOS, CD11b, F4/80 and CD206 by immunohistochemistry. The administration of fractions <30 kDa, 1 and 4 reduced hypernociception and acute and chronic cellular influx in the ZyA model. Transfection with both plasmids, Aba-B or F, significantly reduced the influx of cells in both the zymosan and acute and chronic mBSA arthritis models. Reduction of synovitis, mast cell numbers, joint damage and iNOS expression were observed. Levels of CXCL1, IFN-γ, IL-1β and IL-6 were reduced. However, IL-10 levels did not increase. Surprisingly, IL-5 levels were significantly reduced after plasmid injection. Plasmids increased the expression of M2 macrophages in the synovium. A qPCR analysis showed the expression of peptides in the thigh muscles of mice that received the plasmids. This is the first demonstration that peptide sequences derived from A. suum promote anti-inflammatory activity in arthritis models, and that it is associated with reduced release of inflammatory cytokines. Peptides promoted a change in the differentiation of macrophages in the synovial membrane at the same time, decreasing the release of IL-5. Despite the complex modulating mechanisms of helminths of inflammation, the data still support a protective effect of helminth products in chronic arthritis. / A artrite reumatoide (AR) é uma doença inflamatória autoimune crônica com etiopatogenia multifatorial, envolvendo mecanismos genéticos, imunológicos e ambientais, cujo diagnóstico e tratamento tardio influenciam negativamente na prevalência e gravidade da doença. Portanto, pacientes que residem em países com baixa renda deveriam apresentar um aumento da morbidade e / ou mortalidade por essas doenças. Curiosamente, a evolução parece ser semelhante, se não menos grave, à encontrada em populações com melhores índices socioeconômicos. A hipótese higiênica sugere relação inversa entre a distribuição global de doenças autoimunes e infecções parasitárias, onde helmintos tendem a estimular o desenvolvimento de respostas Th2, além de suprimir respostas Th-1 e Th-17. O objetivo deste estudo foi isolar componentes no extrato de Ascaris suum e caracterizar seu mecanismo antiinflamatório em modelos de artrite experimental. O extrato bruto de Ascaris suum foi separado em frações > 30 e <30 kDa. Foram obtidas quatro frações menores (1-4), na fração <30 kDa, por eletroforese nativa. Na fração 1, foi identificada a proteína ABA-1, por espectrometria de massa, e após digestão proteica com tripsina, sete peptídeos foram isolados (Aba-A – G). A sequência genômica dos peptídeos de Aba-B e Aba-F foi inserida em um plasmídeo comercial (VR2-001-TOPO ™). Os plasmídeos foram clonados e expandidos em E. coli e a extração do DNA plasmidial foi realizada por kit comercial (Qiagen™). Camundongos Swiss machos receberam extrato bruto de A. suum (1mg), >30 kDa , <30kDa (50ng) ou frações 1, 2, 3 e 4 (1μL), diluídos em salina estéril (200 μL), v.o., 30 min antes da injeção de Zy (0,1mg) i.a. Hipernocicepção foi avaliada pelo von Frey eletrônico e o influxo celular foi avaliado no fluido articular 6h ou sete dias após zymosan i.a. Camundongos Swiss e Balb/c machos, submetidos a artrite induzida por zymosan AZy e induzida por antígeno (AIA), respectivamente, receberam plasmídeos recombinantes de Aba-B ou Aba-F (50μg/30μL) na parte posterior de cada coxa traseira. O influxo celular foi avaliado 6h ou sete dias (AZy) e 7h ou 14 dias (AIA) após o estímulo i.a. Citocinas foram avaliadas no líquido sinovial. Sinovite e dano articular foram avaliados por H&E e safranina O, respectivamente, e número de mastócitos por azul de toluidina. Níveis de citocinas (CXCL-1, IL-1β, IFN-γ, IL-5, IL-6 e IL-10) foram avaliadaos por ELISA e a expressão de iNOS, CD11b, F4/80 e CD206 por imunohistoquímica. A administração das frações <30 kDa, 1 e 4 reduziu a hipernocicepção e influxo celular agudo e crônico no modelo de AZy. A transfecção com ambos os plasmídeos, Aba-B ou F, reduziu significativamente o influxo de células em ambos os modelos de artrite por zymosan e mBSA agudo e crônico. Foram observados a redução da sinovite, número de mastócitos, do dano articular e expressão de iNOS. Níveis de CXCL1, IFN-γ, IL-1β e IL-6 foram reduzidos. No entanto, os níveis de IL-10 não aumentaram. Surpreendentemente, os níveis de IL-5 foram significativamente reduzidos após a injeção de plasmídeos. Plasmídeos aumentaram a expressão de macrófagos M2 na sinóvia. Uma análise quantitativa por PCR mostrou a expressão de peptídeos Aba-B e Aba-F nos músculos da coxa de camundongos que receberam os plasmídeos. Esta é a primeira demonstração de que sequências peptídicas derivadas de A. suum promovem a atividade anti-inflamatória em modelos de artrite, e que está associada à redução da liberação de citocinas inflamatórias. Esses peptídeos promoveram mudança na diferenciação de macrófagos na membrana sinovial ao mesmo tempo, diminuindo a liberação de IL-5. Apesar dos complexos mecanismos de modulação de helmintos de inflamação, os dados suportam ainda um efeito protetor dos produtos de helmintos em modelos de artrite crônica.

Made available in DSpace on 2016-04-04T12:31:24Z (GMT). No. of bitstreams: 2 125.pdf: 3528812 bytes, checksum: 1ed3dd48fc984e0873f03c398abf2774 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2015 / Os estudos paleoparasitológicos se caracterizam pela busca de parasitos em material arqueológico e paleontológico. Em geral, a maioria das pesquisas gira em torno de material de épocas mais recentes, principalmente relacionados a grupos humanos, que não chegam a datações de um milhão de anos. A busca por explicações mais antigas em relação ao surgimento das parasitoses nos leva aos animais extintos, que revelam nos vestígios deixados por eles, os parasitos que circulavam antes do surgimento dos seres humanos. Esse trabalho objetivou identificar parasitos que por ventura fossem encontrados em vestígios de animais extintos de diferentes idades geológicas. Então, 32coprólitos de 6 hospedeiros diferentes (caprino, hiena, duas espécies de peixes, cinodonte e dicinodonte), foram analisados nessa pesquisa, com datações que compreendem do Quaternário ao Triássico (10 mil a 240 milhões de anos antes do presente). Várias técnicas de recuperação de material mineralizado e dessecado foram utilizadas, de acordo com que cada coprólito foi preservado. Se mineralizado, técnicas com ácido e aparelho de ultrassom possibilitaram a análise, já para os coprólitos dessecados, foi feita apenas a reidratação com fosfato trissódico. Amostras de 4 hospedeiros foram positivas para parasitos. Cisto de protozoário em caprino, dois ovos de helmintos em coprólitos de hiena, um nematoda em dicinodonte, foram encontrados. Além de um ascarídeo e um oxyurídeo em coprólito de cinodonte, um réptil mamaliforme pertencente a um clado que originou os mamíferos, que apresentaram dois parasitos comumente achados em mamíferos modernos. Portanto, esses achados permitem que respostas sejam respondidas para compreender melhor a relação parasito hospedeiro-ambiente em diferentes épocas da história da vida. / Paleoparasitological studies are characterized by the search for parasites in archaeological and paleontological material. In general, most research centers around more recent material, mainly related to human groups, which doesn‟t reach the dating ofa million years. The search for older explanations in relation to the emergence of parasites leads us to extinct animals, which reveal on the traces left by them, the parasites that circulates before the appearance of humans. This study aimed to identifyparasites that were found in traces of extinct animals of different geological ages.Thereby, 32 coprolites 6 different hosts (goat, hyena, two species of fish, cynodont anddicynodonte) were analyzed in this study, with dating comprising from Quaternary toTriassic (10 thousands to 240 million years before present). Several techniques forrecovery of mineralized and dried material were used in accordance with the way eachcoprolite was preserved. If mineralized, techniques with acid and ultrasound deviceenabled the analysis, and for the disiccated coprolites, it was only made rehydrationwith trisodium phosphate. Samples of 4 hosts were positive for parasites. Protozoan cystin goats, two eggs of helminths in coprolites of hyena and nematode in dicynodonte, were found. In addition, an ascarid and an oxyurid egg in a coprolites of cynodont, amamaliforme reptile that belongs to a monophiletic clade that originated mammals,which showed two parasites commonly found in modern mammals. Therefore, thesefindings allow to fill gaps to understand the host-parasite-environment relationship better at different times in the history of life. (AU)^ien