Orientador: Sônia Regina Panzarini Barioni / Co-orientador: Cláudio Aparecido Casatti / Banca: Jéssica Lemos Gulinelli / Banca: Maurício Zardo / Banca: Idelmo Rangel Garcia Junior / Banca: Celso Koogi Sonoda / Resumo: Recentes investigações sugerem que a deficiência de estrógeno, contribue para alteração nos tecidos periodontais. Considerando o estudo do traumatismo dento-alveolar em indivíduos com essa condição, o objetivo do trabalho foi analisar o efeito da ovariectomia sobre os tecidos periodontais após o reimplante dentário imediato por meio da análise histomorfométrica, imunoistoquímica e micro-ct em ratas. Oitenta ratas Wistar (Rattus norvegicus, albinus) com ciclos estrais normais foram divididas aleatoriamente em dois grupos: Grupo ovariectomizadas (OVX), e Grupo Sham (grupo controle). Após 2 meses da ovariectomia, foi realizado extração dos incisivos superiores seguido de reimplante dentário imediato e os animais sofreram eutanásia aos 28, 45 e 60 dias após o reimplante. Foi realizada a análise histomorfométrica e imunoistoquímica com avaliação das proteínas PCNA, OPG, CASPASE-3, RUNX-2 e TRAP. Em todos os grupos houve reinserção do ligamento periodontal no tecido ósseo e cemento e a imunorreatividade à PCNA foi evidente em células periodontais independente do grupo Sham ou OVX. A reabsorção radicular externa esteve mais acentuada a partir dos 45 dias com células imunorreativas à TRAP presentes na superfície óssea e dentária. Todavia, uma considerável parcela também estava dispersa no ligamento periodontal em todos os grupos. Não houve diferença estatisticamente significante entre os grupos para as proteínas PCNA e TRAP. Já a análise histomorfométrica revelou diferença estatisticamente significante entre os grupos nos detalhes histomorfométricos do ligamento periodontal e raíz dentária para os sub-itens de intensidade... / Abstract: Recent researchs suggest estrogen deficiency leads to changes in periodontal tissues. In order to study dental traumatology under this condition, the aim of the study was analyze the effects of ovariectomy on periodontal tissues following immediate tooth replantation through histomorphometric, immunohistochemistry and μ-ct analysis in rats. Eighty Wistar rats (Rattus norvegicus albinos) with normal estrous cycles were randomly divided into two groups (n = 20 per group): 1) Group ovariectomized (OVX), and 2) Sham group (control group). After two months, the rats were followed by extraction of upper right incisor and immediate replantation. The rats were sacrificed immediately 28, 45 and 60 days after tooth reimplantation. Histomorphometric and immunohistochemical analysis was performed by evaluation of PCNA, OPG, CASPASE-3, RUNX-2 and TRAP proteins. Periodontal ligament was reinseted in the bone and cementum and the immunohistochemical analysis revealed PCNA expression in the periodontal ligament independent of Sham or OVX group. At 45 days, the external root resorption was greater with expression of TRAP proteins in the bone and tooth surface. However, in both groups, were also dispersed in the periodontal ligament. There was no statistically significant difference between the groups for the PCNA and TRAP proteins. The histomorphometric analysis showed statistically significant difference between groups in the histomorphological details of periodontal ligament and root resorption for the sub-items: intensity of chronic inflammatory infiltrate at 60 days (p <0.01), the organization of the periodontal ligament at 45 days (p <0:05) and depth... / Doutor

Objective: To assess the knowledge of Fire Services staff regarding the management of tooth avulsion. Methods: A web-based survey was designed and sent to Fire Services staff of two local communities. Results: Of all 340 respondents, 42% previously experienced case(s) of tooth avulsion. Only 7% of the study participants had complete knowledge about tooth avulsion. The relationship between the knowledge and information previously received was statistically significant. 84% of the respondents were interested in receiving further information about tooth avulsion and on-line education was selected as the most preferred method. Conclusion: The study demonstrates the lack of adequate knowledge and training among Fire Services personnel about emergency management of avulsed tooth injuries. Fire services personnel would benefit from training regarding the management of tooth avulsion injuries, given the high likelihood of them being the first responders and encountering such emergencies.

Objective: To assess the knowledge of Fire Services staff regarding the management of tooth avulsion. Methods: A web-based survey was designed and sent to Fire Services staff of two local communities. Results: Of all 340 respondents, 42% previously experienced case(s) of tooth avulsion. Only 7% of the study participants had complete knowledge about tooth avulsion. The relationship between the knowledge and information previously received was statistically significant. 84% of the respondents were interested in receiving further information about tooth avulsion and on-line education was selected as the most preferred method. Conclusion: The study demonstrates the lack of adequate knowledge and training among Fire Services personnel about emergency management of avulsed tooth injuries. Fire services personnel would benefit from training regarding the management of tooth avulsion injuries, given the high likelihood of them being the first responders and encountering such emergencies.

An Alpine Fault Earthquake has the potential to cause significant disruption across the Southern Alps of the South Island New Zealand. In particular, South Island river systems may be chronically disturbed by the addition of large volumes of sediment sourced from coseismic landsliding. The Taramakau River is no exception to this; located north of Otira, in the South Island of New Zealand, it is exposed to natural hazards resulting from an earthquake on the Alpine Fault, the trace of which crosses the river within the study reach. The effects of an Alpine Fault Earthquake (AFE) have been extensively studied, however, little attention has been paid to the effects of such an event on the Taramakau River as addressed herein. Three research methods were utilised to better understand the implications of an Alpine Fault Earthquake on the Taramakau River: (1) hydraulic and landslide data analyses, (2) aerial photograph interpretation and (3) micro-scale modelling. Data provided by the National Institute of Water and Atmospheric Research were reworked, establishing relationships between hydraulic parameters for the Taramakau River. Estimates of landslide volume were compared with data from the Poerua landslide dam, a historic New Zealand natural event, to indicate how landslide sediment may be reworked through the Taramakau valley. Aerial photographs were compared with current satellite images of the area, highlighting trends of avulsion and areas at risk of flooding. Micro-scale model experiments indicated how a braided fluvial system may respond to dextral strike-slip and thrust displacement and an increase in sediment load from coseismic landslides. An Alpine Fault Earthquake will generate a maximum credible volume of approximately 3.0 x 108 m3 of landslide material in the Taramakau catchment. Approximately 15% of this volume will be deposited on the Taramakau study area floodplain within nine years of the next Alpine Fault Earthquake. This amounts to 4.4 x 107 m3 of sediment input, causing an average of 0.5 m of aggradation across the river floodplains within the study area. An average aggradation of 0.5 m will likely increase the stream height of a one-in-100 year flood with a flow rate of 3200 m3/s from seven metres to 7.5 m overtopping the road and rail bridges that cross the Taramakau River within the study area – if they have survived the earthquake. Since 1943 the Taramakau River has shifted 500 m away from State Highway 73 near Inchbonnie, moving 430 m closer to the road and rail. Paleo channels recognised across the land surrounding Inchbonnie between the Taramakau River and Lake Brunner may be reoccupied after an earthquake on the Alpine Fault. Micro-scale modelling showed that the dominant response to dextral strike-slip and increased ‘landslide’ sediment addition was up- and downstream aggradation separated by a localised zone of degradation over the fault trace. Following an Alpine Fault Earthquake the Taramakau River will be disturbed by the initial surface rupture along the fault trace, closely followed by coseismic landsliding. Landslide material will migrate down the Taramakau valley and onto the floodplain. Aggradation will raise the elevation of the river bed promoting channel avulsion with consequent flooding and sediment deposition particularly on low lying farmland near Inchbonnie. To manage the damage of these hazards, systematically raising the low lying sections of road and rail may be implemented, strengthening (or pre-planning the replacement of) the bridges is recommended and actively involving the community in critical decision making should minimise the risks of AFE induced fluvial hazards. The response of the Taramakau River relative to an Alpine Fault Earthquake might be worse, or less severe or significantly different in some way, to that assumed herein.

Resumo: Este artigo relata a experiência da utilização da técnica de grupo focal para coletar dados de pesquisa em Odontologia. Esta técnica tem como objetivo utilizar a Técnica do Grupo Focal para adequação da terminologia do questionário sobre reimplante dentário, direcionado a escolares. Para isso, 2 grupos, com 10 alunos cada, sorteados aleatoriamente, foram formados com alunos a 6ª série de 2 escolas da rede pública da cidade de Araçatuba, SP, os quais foram abordados os seguintes temas: trauma dentário, com ênfase em avulsão e reimplante dentário. Após o desenvolvimento das discussões, foi possível observar que a maioria dos escolares desconhece o real sentido da palavra trauma dentário, associando este termo à "cárie no dente", "mau hálito", "dor de dente", "chupar o dedo", "ir ao dentista". As exceções foram os alunos que sofreram ou presenciaram este tipo de acidente; o mesmo deu-se para os demais termos técnicos analisados. Pode-se concluir que a Técnica do Grupo Focal como metodologia qualitativa é muito importante na adequação da terminologia de questionários, sendo possível individualizá-los, deixando-o compatível à realidade da população alvo a ser pesquisada. / Abstract: This article reports the experience of the use of the technique of focus group to collect data for research in Dentistry. This technique is intended to adapt the terminology of a questionnaire in preparation on reimplante dental. For this, two groups were conducted for discussion, addressing the theme: dental trauma, with emphasis on avulsion and reimplante dental. After the development of the discussions, it was possible to observe that most school know the real meaning of the word dental trauma, linking this term to "cárie in the tooth," "bad breath", "pain in the tooth," "sucking the finger," "go to the dentist." The exceptions were the students who suffered or presenciaram this type of accident, it has been for other technical terms analyzed. In possession of the descriptions made by the school were unable to prepare and / or modify the terms of any of the items of the questionnaire, adapting to the reality of the target population to search. / Orientador: Maria Lúcia Marçal Mazza Sundefeld / Coorientador: Dalton Francisco de Andrade / Banca: Wilson Roberto Poi / Banca: José Eduardo Corrente / Mestre

Orientador: Wilson Roberto Poi / Banca: Sônia Regina Panzarini / Banca: Maurício Zardo / Resumo: A elaboração de um diagnóstico preciso e de um plano de tratamento adequado pode constituir uma tarefa bastante complexa, especialmente nas avulsões dentárias, pois uma série de variáveis está envolvida nestes casos. Além do conhecimento técnico e da experiência clínica, direcionados à qualidade do tratamento, a transmissão de informações à população pode influenciar de forma favorável na sobrevida dos dentes reimplantados. A proposta deste trabalho é analisar os procedimentos empregados por 100 cirurgiões-dentistas no atendimento dos casos de avulsão dentária. Para tanto, a partir de um questionário descritivo, foram abordados questões referentes ao perfil dos profissionais entrevistados e procedimentos empregados nos casos de avulsão, educação e orientação dos pacientes. Cem questionários devidamente preenchidos foram selecionados e os dados obtidos foram submetidos à análise descritiva, enquanto o teste estatístico foi aplicado para demonstrar freqüências e o nível de significância entre variáveis (teste qui-quadrado). De acordo com os resultados, verifica-se que esse tipo de trauma faz parte da rotina odontológica de 15 cirurgiões-dentistas, muito embora 71 tenham relatado alguma experiência com dentes avulsionados. Grandes deficiências foram encontradas com relação ao tratamento da superfície radicular e ajuste oclusal. Achados positivos foram aqueles relacionados ao tratamento do alvéolo dentário e terapêutica medicamentosa empregada, bem como à educação e orientação dos pacientes (tempo extra-alveolar, meio de conservação, manipulação do dente avulsionado, reimplante pelo próprio paciente). Foi possível concluir que 47,5% dos procedimentos empregados pelos 100 cirurgiões-dentistas entrevistados são adequados e que 87,7% das orientações transmitidas aos pacientes são favoráveis, fatores que podem interferir positivamente no prognóstico do reimplante dentário. / Abstract: An accurate diagnosis and an adequate treatment plan may constitute a very complex task, particularly in teeth avulsion, because a series of variables are involved. In addition to the technical knowledge and clinical experience directed towards the quality of the treatment, patient education may influence favorably the survival of replanted teeth. The aim of this study is to analyze the procedures used in the management of dental avulsions by 100 dental surgeons. Thus, by means of a descriptive questionnaire, we obtained information about the profile of the professionals interviewed, the procedures used in avulsion cases and patient orientation and education. One hundred properly filled out questionnaires were selected. Descriptive statistics were used for the data while the chi-square statistic was employed to test the significance of association between variables. According to the results, this kind of trauma is part of the routine of 15 dental surgeons, although 71 have reported some experience with avulsed teeth. Great deficiencies were found regarding root surface treatment and occlusal adjustment. Positive findings were related to the socket treatment, adjunctive theraphy and patient education and orientation (extra-alveolar period, storage medium, manipulation of the avulsed tooth, patient’s himself replantation). It was possible to conclude that 47,5% of the procedures reported by 100 professionals interviewed are adequate and 87,7% patients education are favorable, a fact that can interfere positively with teeth replantation prognosis. / Mestre

Orientador: Carlos Henrique Ribeiro Camargo / Banca: Marcia Carneiro Valera Garakis / Banca: Giulio Gavini / Banca: Aletéia Massula de Melo Fernandes / Banca: Frederico Canato Martinho / Resumo: O reimplante tardio de dentes avulsionados é uma realidade dentro dos consultórios odontológicos. Na tentativa de se restabelecer a arquitetura e a função do ligamento periodontal, além de se evitar a anquilose, evitando ou modulando a reabsorção por substituição, a superfície radicular dos dentes avulsionados pode ser tratada com diversas substâncias, dentre elas o EDTA (ácido etilenodiamino tetra-acético) a 17%, sempre visando uma menor chance de ocorrer reabsorção inflamatória e por substituição. Sendo assim, o objetivo deste estudo foi avaliar a eficácia do tratamento de discos da superfície vestibular de raízes de dentes bovinos, com EDTA 17%, com Emdogain®, com ácido hialurônico e com colágeno, através de testes de viabilidade celular, quantificação das citocinas IL-1ß, IL-6, IL-8, IL-10 e TNF-α por ensaio ELISA, além da ilustração por microscopia eletrônica de varredura da adesão dos fibroblastos sobre os discos de dentina. Foram cortados 155 discos de dentina, com 4,5 mm de diâmetro, da face vestibular da superfície radicular de dentes bovinos, previamente raspados ou não com cureta periodontal. Os discos foram regularizados, limpos e autoclavados. Os espécimes foram tratados com as substâncias preconizadas e colocados em placas de 96 poços onde foram semeadas células de culturas primárias de fibroblastos humanos de ligamento periodontal, que ficaram em contato com os discos por 48 horas. A sobrevivência e a viabilidade celular na superfície dos discos foram avaliadas através do ensaio de XTT. A microscopia eletrônica de varredura foi utilizada para verificar a adesão de fibroblastos à superfície dos discos. A detecção e quantificação das citocinas foram realizadas pelo teste ELISA. Os dados foram submetidos à análise estatística pelo teste ANOVA e Tukey (p < 0,05). Os resultados obtidos mostraram que o uso de EDTA 17% por 5 minutos sem agitação e o ácido hialurônio... / Abstract: The delayed reimplantation of avulsed teeth is a reality in dental offices. In an attempt to restore architecture and function of the periodontal ligament, avoiding ankylosis, preventing or modulating the replacement resorption, avulsed teeth root surface may be treated with various substances including EDTA (ethylenediaminetetraacetic acid) to 17%, always seeking a lower chance of occurring inflammatory or replacement resorption. Thus, the aim of this study was to evaluate the effectiveness of the treatment of discs cutted of the vestibular surface of bovine teeth roots with EDTA 17% with Emdogain® with hyaluronic acid and collagen through cell viability tests, quantification of cytokines IL -1ß, IL-6, IL-8, IL-10 and TNF-α by ELISA assay and, in addition, it was taken Illustrations by scanning electron microscopy of the accession of fibroblasts on the dentin disks. 155 dentin discs with 4,5 mm in diameter will be cut from the vestibular surface of the root surface of bovine teeth, previously scraped or not with periodontal curette. The discs were regularized, cleaned and autoclaved. The specimens were treated with the substances advocated and placed in 96 well plates are seeded in which primary cell cultures of human periodontal ligament fibroblasts that were in contact with the discs for 48 hours. The survival and cell viability on the surface of the disks are evaluated by XTT assay. The scanning electron microscopy was used to verify adherence of fibroblasts to the surface of the discs. Detection and quantification of cytokines were done by ELISA assay. The data were statistically analyzed by ANOVA and Tukey (p < 0.05). The results showed that the use of 17% EDTA for 5 minutes without stirring was more effective than the other protocols (p <0.05) and combined with hyaluronic acid was less cytotoxic promoting better cell viability. It was not possible to ensure the fibroblast of the periodontal... / Doutor

Made available in DSpace on 2014-08-27T14:36:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-09-13Bitstream added on 2014-08-27T15:57:09Z : No. of bitstreams: 1 000750428.pdf: 3561830 bytes, checksum: fc7aef2e36f774b1718408ccdc2728e5 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Recentes investigações sugerem que a deficiência de estrógeno, contribue para alteração nos tecidos periodontais. Considerando o estudo do traumatismo dento-alveolar em indivíduos com essa condição, o objetivo do trabalho foi analisar o efeito da ovariectomia sobre os tecidos periodontais após o reimplante dentário imediato por meio da análise histomorfométrica, imunoistoquímica e micro-ct em ratas. Oitenta ratas Wistar (Rattus norvegicus, albinus) com ciclos estrais normais foram divididas aleatoriamente em dois grupos: Grupo ovariectomizadas (OVX), e Grupo Sham (grupo controle). Após 2 meses da ovariectomia, foi realizado extração dos incisivos superiores seguido de reimplante dentário imediato e os animais sofreram eutanásia aos 28, 45 e 60 dias após o reimplante. Foi realizada a análise histomorfométrica e imunoistoquímica com avaliação das proteínas PCNA, OPG, CASPASE-3, RUNX-2 e TRAP. Em todos os grupos houve reinserção do ligamento periodontal no tecido ósseo e cemento e a imunorreatividade à PCNA foi evidente em células periodontais independente do grupo Sham ou OVX. A reabsorção radicular externa esteve mais acentuada a partir dos 45 dias com células imunorreativas à TRAP presentes na superfície óssea e dentária. Todavia, uma considerável parcela também estava dispersa no ligamento periodontal em todos os grupos. Não houve diferença estatisticamente significante entre os grupos para as proteínas PCNA e TRAP. Já a análise histomorfométrica revelou diferença estatisticamente significante entre os grupos nos detalhes histomorfométricos do ligamento periodontal e raíz dentária para os sub-itens de intensidade... / Recent researchs suggest estrogen deficiency leads to changes in periodontal tissues. In order to study dental traumatology under this condition, the aim of the study was analyze the effects of ovariectomy on periodontal tissues following immediate tooth replantation through histomorphometric, immunohistochemistry and ?-ct analysis in rats. Eighty Wistar rats (Rattus norvegicus albinos) with normal estrous cycles were randomly divided into two groups (n = 20 per group): 1) Group ovariectomized (OVX), and 2) Sham group (control group). After two months, the rats were followed by extraction of upper right incisor and immediate replantation. The rats were sacrificed immediately 28, 45 and 60 days after tooth reimplantation. Histomorphometric and immunohistochemical analysis was performed by evaluation of PCNA, OPG, CASPASE-3, RUNX-2 and TRAP proteins. Periodontal ligament was reinseted in the bone and cementum and the immunohistochemical analysis revealed PCNA expression in the periodontal ligament independent of Sham or OVX group. At 45 days, the external root resorption was greater with expression of TRAP proteins in the bone and tooth surface. However, in both groups, were also dispersed in the periodontal ligament. There was no statistically significant difference between the groups for the PCNA and TRAP proteins. The histomorphometric analysis showed statistically significant difference between groups in the histomorphological details of periodontal ligament and root resorption for the sub-items: intensity of chronic inflammatory infiltrate at 60 days (p <0.01), the organization of the periodontal ligament at 45 days (p <0:05) and depth... / FAPESP: 10/12593-8 / FAPESP: 12/08254-98

Resumo: O reimplante dentário é a melhor opção para o tratamento da avulsão. Todavia, período extrabucal prolongado ocasiona danos ao ligamento periodontal, que desencadeiam processos de reabsorção radicular externa. O objetivo desse estudo foi avaliar histomorfometricamente, a viabilidade da solução de própole e a de flúor no controle da reabsorção radicular externa no processo de reparo do reimplante tardio. Trinta ratos (Rattus norvegicus albinus, variedade Wistar) tiveram seu incisivo superior direito extraído. Os dentes foram mantidos em meio seco por 60 minutos. Após esse período a papila dentária, o órgão do esmalte e o ligamento periodontal removidos com lâmina de bisturi e a polpa foi extirpada. Os dentes foram divididos em três grupos experimentais: grupo I - dentes imersos em 20 ml de solução de soro fisiológico; grupo II - dentes imersos em 20 ml de solução de fluoreto de sódio fosfato acidulado a 2%; grupo III - imersão dos dentes em 20 ml de solução de própole a 15%. Após 10 minutos de imersão nas soluções, os canais radiculares foram secos e preenchidos com pasta de hidróxido de cálcio e os dentes reimplantados. A eutanásia dos animais ocorreu 60 dias após o ato operatório. Os resultados mostraram presença de reabsorção inflamatória, por substituição e anquilose nos três grupos experimentais, não apresentando diferença significativa quando submetidos à análise comparativa, o que levou a conclusão de que o tratamento da superfície radicular com própole se mostrou semelhante ao do flúor com relação à reabsorção radicular externa. Apesar de não apresentarem diferença estatisticamente significante, houve maior quantidade de reabsorção inflamatória do soro fisiológico quando comparado ao flúor e à própole. / Abstract: Tooth replantation is the best option for treatment of an avulsed tooth. Nevertheless, an extended extraoral period damages the periodontal ligament, and results in external root resorption. The purpose of this study was to assess by histologic and histometric analysis, the influence of propolis and the fluoride solution used as root surface treatment on the healing process after delayed tooth replantation. Thirty Wistar (Rattus norvegicus albinus) were submitted to extraction of their upper right incisor. The teeth were maintained in a dry environment for 60 minutes. After this time the pulp was extirpated and the papilla, enamel organ and periodontal ligament were removed with scalpel. The teeth were divided in to three experimental groups: group I - teeth immersed in 20 ml of physiologic saline; group II - teeth immersed in 20 ml of 2% acidulated phosphate sodium fluoride; group III - teeth immersed in 20 ml of 15% propolis. After 10 minutes of immersion in the solutions, the root canals were dried and filled with calcium hydroxide paste and the teeth were replanted. The animals were euthanized 60 days after replantation. The results showed the similar external root resorption was seen in the propolis and fluoride groups. Teeth treated with physiologic saline tended to have more inflammatory root resorption compared to those treated with fluoride or propolis. However, the comparative analysis did not reveal any statistically signifant differences between the treatment modalities when used for delayed tooth replantation. / Orientador: Sônia Regina Panzarini Barioni / Coorientadora: Cristiane Mara Ruiz de Sousa Fattah / Banca: Ana Paula Farnezi Bassi / Banca: Celso Koogi Sonoda / Mestre

Made available in DSpace on 2014-06-11T19:23:45Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-08-09Bitstream added on 2014-06-13T20:51:00Z : No. of bitstreams: 1 hamanaka_ef_me_araca.pdf: 1323688 bytes, checksum: dd4fbda158212ee0b954b2dbb95c04a7 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / A manutenção da vitalidade do ligamento periodontal cementário é de extrema importância para o sucesso do reimplante dentário. Minimizar o período de exposição do dente avulsionado ao meio seco ou mantê-lo em meio úmido, se constitui em importante conduta para o tratamento. Recentemente, o resveratrol tem sido bastante estudado, em razão das suas propriedades antioxidantes e da sua capacidade de prolongar a vida de diversos organismos desde as leveduras até os mamíferos. Portanto, o objetivo deste trabalho é avaliar a manutenção da vitalidade do ligamento periodontal cementário armazenado em resveratrol. Para isso foram empregados incisivos superiores e inferiores de sete macacos, totalizando 56 dentes. No grupo I os dentes foram extraídos e imediatamente imersos em solução fixadora. Nos grupos II a VI os dentes foram imersos em 50mL de solução de resveratrol em propilenoglicol (5 μM/L) durante 1, 2, 6, 12 e 24 horas, respectivamente. No grupo VII os dentes foram mantidos em meio seco por 6 horas. Parte dos cortes obtidos foi corada em H.E. para estudo histomorfológico e outra parte para análise imunoistoquímica utilizando anticorpos primários contra osteopontina e caspase. Os resultados demonstraram que nos grupos imediato e resveratrol 12 horas houve presença de moderado número de fibroblastos, alguns macrófagos e linfócitos. Nos grupos resveratrol 1, 2, 6 e 24 horas houve presença de pequeno número de fibroblastos e discreta vascularização. O grupo seco apresentou ausência de vasos sanguíneos e fibroblastos em degeneração. Houve imunomarcação moderada de osteopontina nos grupos imediato, resveratrol 12 e 24 horas. Nos demais grupos a imunomarcação variou de ausente a leve. Quanto à caspase, foi observada imunomarcação moderada nos grupos resveratrol... / The maintenance of vitality of the cement periodontal ligament is important for success of tooth reimplantation. It has been suggested to minimize the period of exposure of the avulsed tooth to dry environment or keep it in wet storage. Recently, resveratrol has been extensively studied due to its antioxidant properties and ability to prolong the lives of several organisms since yeast to mammals. The aim of this study is to evaluate the maintenance of vitality of the cement periodontal ligament stored in resveratrol. The analysis was conducted using maxillary and mandibular incisors of seven monkeys, total of 56 teeth. In group I, the teeth were extracted and immediately immersed in fixative solution. In groups II to VI, the teeth were immersed in 50ml of solution of resveratrol in propylene glycol (5 μM/L) for 1, 2, 6, 12 and 24 hours; respectively. In group VII, the teeth were maintained in dry environment for 6 hours. Some cuts were stained with HE for histomorphological analysis while some cuts were submitted to immunohistochemical analysis using primary antibody against osteopontin and caspase. The results demonstrated that in the groups immediate and resveratrol 12 hours showed presence of a moderate number of fibroblasts, some macrophages and lymphocytes. In the groups resveratrol 1, 2, 6 and 24 hours showed presence of small numbers of fibroblasts and mild vascularization. The dry group showed absence of blood vessels and fibroblasts in degeneration. There was moderate immunomarcation of osteopontin in the groups immediate, and resveratrol 12 and 24 hours. In the other groups, immunomarcation ranged from absent to light. Considering the caspase, it was observed moderate immunomarcation in the groups resveratrol 1, 2, 6 and 24 hours. In the remaining groups, immunomarcation ranged from absent to light... (Complete abstract click electronic access below)