Numa área experimental de 25,2 ha formada com o capim-braquiarão (Brachiaria brizantha (Hochst) Stapf.) cv. Marandu e localizada no Campus da USP em Pirassununga/SP, durante o período de janeiro a julho de 2004, conduziu-se a presente pesquisa pela Faculdade de Zootecnia e Engenharia de Alimentos (FZEA/USP) com os seguintes objetivos: 1) Determinar as frações de carboidratos (A - açúcares solúveis com rápida degradação ruminal; B1- amido e pectina; B2 - parede celular com taxa de degradação mais lenta; C - fração não digerida) e as frações protéicas (A - NNP; B1 - peptídeos e oligopeptídeos; B2 - proteína verdadeira; B3 - NFDN; C - NIDA) na forragem da gramínea, baseados nas equações utilizadas pelo método de Cornell; 2) Relacionar outras variáveis com as medições em campo de experimentos paralelos e dados de elementos de clima com as frações protéicas e de carboidratos com o auxílio de um modelo computacional baseado em redes neurais artificiais (RNA). O delineamento foi em blocos completos e casualizados, com quatro tratamentos (ofertas de forragem de 5, 10, 15 e 20% - kg de massa seca por 100 kg de peso animal.dia) e quatro repetições. Cada bloco era dividido em quatro unidades experimentais de 1,575 ha, com cinco piquetes de 0,315 ha cada. Os animais eram manejados em cada unidade experimental em lotação rotacionada, com períodos de descanso de 28 dias no verão e 56 dias no inverno e período de ocupação de 7 dias, respectivamente. As amostras eram colhidas 2 dias antes da entrada dos animais à altura do resíduo do pastejo anterior. Foram determinados produção de massa seca (MS), alturas de pré e pós pastejo, fibras em detergente ácido (FDA) e neutro (FDN), sacarose, amido, lignina, extrato etéro (EE), carboidrato totais (CHO), carboidratos não estruturais (CNE), frações A, B1, B2 e C de carboidratos, proteína bruta (PB), frações A, B1, B2, B3 e C de proteínas e análise de uma rede neural artificial para uma predição dos teores de FDA, fibra em detergente neutro, PB e as frações protéicas. A produção de massa seca (MS) foi significativa, quando se estudou os efeitos da oferta de forragem (p<0,05), ciclo de pastejo (p<0,05) e da interação oferta de forragem x ciclo de pastejo (p<0,05). A maior produção foi no mês de março, quando se alcançou a média de 16140 kg MS/há para o oferta de 20%. Os teores de FDA foram significativos, quando se estudou a oferta de forragem (p<0,05) e seus maiores. Os teores médios da fibra em detergente neutro foram de 66,3 e 64,7% no verão e inverno respectivamente. Houve diferenças significativas para PB, quando se estudou a oferta de forragem (p<0,05), sendo seus teores médios de maior valor na OF a 5%. Observa aumento dos CNE em função de lâminas e colmos ao longo das estações do ano com interação no CP x OF (p<0,05) e seus maiores valores foram encontrados no ciclo de pastejo 3 na oferta de forragem 5%. Os teores de CHO totais apresentaram diferenças (p<0,10) em função da oferta de forragem, sendo os maiores teores médios encontrados na oferta de forragem de 20%. As frações A e B2 de CHO foram significativas em função da oferta de forragem (p<0,05), enquanto que os maiores teores médios da fração A foram encontrados nos ciclos de pastejo 3 e 4 e das frações B2 (%CHO) no ciclo de pastejo 1. As frações B2 e C de CHO apresentaram-se diferentes (p<0,05) nos ciclos de pastejo, sendo decrescentes para a fração B1 e crescentes para a fração C. As frações A (47%), B1 (11%) e B3 (10%) de proteínas foram significativas nos ciclos de pastejos. Os teores médios da fração B2 de proteínas apresentaram-se semelhantes (p>0,05) e os da fração C de proteínas foram diferentes (p<0,05) nas ofertas de forragem e ciclos de pastejo. Conclui-se que os ciclos de pastejos interferiram em todas as variáveis estudadas e que os teores das frações de proteínas e carboidratos estão dentro da variação (%) encontrada na literatura. A rede neural artificial conseguiu vincular as interações existentes de dados de campo e estimar os valores laboratoriais dentro de erros esperados, permitindo com isso desvincular análises laboratoriais, de qualidade de planta forrageira, à pesquisa agropecuária e com isso obter além de resultados mais rápidos, menor custo de pesquisa. / In a experimental área of 25.2 há formed with capim-braquiarão (Brachiaria brizantha (Hochst) Stapf ) cv. Marandu located in University of São Paulo Campus of Pirassununga/SP, during the period of january to july of 2004 was lead the present recherché for Faculdade de Zootecnia e Engenharia de Alimentos (FZEA/USP) to appetent the following objectives: 1) Determine protein fractions (the NNP; B1 - peptides and oligopepitides; B2 - true protein; B3 - NDF, C - AND) and carbohydrates fractions (soluble sugars with fast rumem degradation); B1(starch and pectin); B2 (cell wall alower degradation rate; C (indigested fraction rate) in the fodder plant of the grass, as it\'s respetive dregadability rate, based on equations using Cornell model. 2) To relate other variables measurements in field to parallel experiments and climate elements to the protein and carbohydrate fractions was used a computacional model based in nets of artificial neural. The randomized complete block design with four treatments (herbage allowance of 5, 10, 15 and 20% - kg of dry mass for 100 kg of animal.dia weight) and four repetitions. Each block was divided in four experimental units of 1,575 ha, with five 0,315 poles of ha each. The animals were management in each experimental unit in rotational grazing capacity, with periods of rest of 28 days in the summer and 56 days in the winter and period of occupation of 7 days, respectively. The samples were harvested 2 days before the entrance of the animals to the height of the residue of pasture previous. Were conducted analysis of production of dry mass (DM), heights daily pay and after grazing, staple fibers in acid detergent (ADF) and neutral (NDF), sacarose, starch, lignina, extract etereo (EE), carbohydrate (CHO), not structural carbohydrate (NSC), fractions A, B1, B2 and C of carbohydrate, crude protein (CP), fractions protein A, B1, B2, B3 and C and analysis of artificial neural network for a prediction of levels of ADF, NDF, CP and protéicas fractions. The dry matter (DM) production was significant for herbage allowance (p<0,05), grazing periods (p<0,05) and interaction between allowances x grazing periods (p<0,05). The righ production was in February 13,352 kg MS/ha. The ADF was significant for allowance and grazing periods (p<0,05), with 34.8%, on summer and 35.9% on winter. The average measured of NDF on summer and winter was 66.3 and 64.7%, respectively. It showed significant differences of PC when studied the allowance (p<0,05) and its average measured on summer and winter was 8,3 and 8,1%, respectively. It observes increase of the CNE in function of blades and stem to the long one of the stations of the year with interaction in grazing periods x herbage allowance and its bigger values had been found in the grazing periods 3 with herbage allowance 5%. The total texts of CHO had presented differences (p<0,10) in function of herbage allowance, being biggest found average texts in herbage allowance of 20%. The fractions and the B2 of CHO had been significant, when studied in function of the herbage allowance (p<0,05) for the fraction A and for fraction B2 (p<0,05); the biggest average texts in % of CHO of the fraction had been found It in the cycles of grazing 3 and 4 and the B2 fractions (%CHO) in the grazing periods 1. Fractions B2 (p<0,05) and C (p<0,05) of CHO had presented significant differences, when studied the factor grazing periods, where the B1 fraction the texts had been diminishing the measure that increased the grazing periods and fraction C the texts had increased the measure that had increased the grazing periods. The A, B1 and B3 protein fraction was significant when was studied the grazing periods and the results were 0,47; 0,11; 0,10 respectively. The B2 fraction was not significant. C fraction was significant when studied the allowance (p<0,05) and grazing periods (p<0,05). It was concluded that the grazing periods had intervened with all the studied 0 variable and that the texts of the protein fractions and carbohydrates are inside of the variation (%) found in literature. The results from lab was used to train and test neural network. With a program developed by neural network in a mult layer perceptron with capacity to predict the parameters of nutrition and nourishing value from parameters of forage plant intrinsic and extrinsic, where it was allowed to disentail lab analysis of forage plant quality on the farm research, to get beyond faster and have less research costs.

No description available.

In this report the influence of the growth conditions on the surface morphology of AlGaN/GaN heterostructures grown on sapphire-based and bulk GaN substrates is nondestructively investigated with focus on the decoration of defects and the surface roughness. Under Ga-rich conditions specific types of dislocations are unintentionally decorated with shallow hillocks. In contrast, under Ga-lean conditions deep pits are inherently formed at these defect sites. The structural data show that the dislocation density of the substrate sets the limit for the density of dislocation-mediated surface structures after MBE overgrowth and no noticeable amount of surface defects is introduced during the MBE procedure. Moreover, the transfer of crystallographic information, e.g. the miscut of the substrate to the overgrown structure, is confirmed. The combination of our MBE overgrowth with the employed surface morphology analysis by atomic force microscopy (AFM) provides a unique possibility for a nondestructive, retrospective analysis of the original substrate defect density prior to device processing.

Nipah virus (NiV) and Hendra virus (HeV) have been identified as the causes of outbreaks of fatal meningitis, encephalitis, and respiratory disease in Australia, Malaysia, Bangladesh, and India from 1994 until 2004. In order to accommodate the unique genomic characteristics of NiV and HeV, a new genus within the family Paramyxoviridae was created, named Henipavirus. NiV encodes two surface glycoproteins: the attachment glycoprotein (G) binds to the cellular receptor for the virus, while the fusion glycoprotein (F) mediates membrane fusion between the virus and cell membranes. Expression of F and G in the same cell results in cell-cell fusion in transfected cell monolayers, while expression of F and G on their own in cell monolayers does not result in fusion. Co-culture of singly-transfected F and G cells also does not result in fusion. Expression of NiV G in transgenic CRFK cells results in resistance to NiV- and HeV-induced cytopathic effect. Additionally, neither NiV nor HeV nucleic acid could be detected in CRFK-NiV G that had been exposed to NiV or HeV. NiV G expression also prevents NiV F+NiV G-mediated cell-cell fusion, but does not affect cell surface expression of either virus receptor, ephrin-B2 and ephrin-B3. Chimeric glycoproteins derived from NiV G and CDV H were constructed and characterized. None of the chimeric glycoproteins were able to fuse when coexpressed with either NiV F or CDV F. Only one of the chimeric glycoproteins (H145/G458) was detected on the cell surface by immunofluorescence assay (IFA). None of the chimeric glycoproteins altered cell surface expression levels of ephrin-B2 and ephrin-B3. Finally, recombinant NiV genomes (rNiV and rNiV eGFPG) were constructed, as well as chimeric CDV genomes with NiV ORF substitutions (rCDV eGFPH NiVFG and rCDV eGFPH NiVMFG). The only chimeric virus that was generated, rCDV eGFPH NiVFG, was assessed for its release from infected cells. rCDV eGFPH NiVFG was poorly released from infected cells without a freeze-thaw cycle, but was also found to induce the cellsurface down-regulation of the viral receptors ephrin-B2 and ephrin-B3. / October 2007

Nipah virus (NiV) and Hendra virus (HeV) have been identified as the causes of outbreaks of fatal meningitis, encephalitis, and respiratory disease in Australia, Malaysia, Bangladesh, and India from 1994 until 2004. In order to accommodate the unique genomic characteristics of NiV and HeV, a new genus within the family Paramyxoviridae was created, named Henipavirus. NiV encodes two surface glycoproteins: the attachment glycoprotein (G) binds to the cellular receptor for the virus, while the fusion glycoprotein (F) mediates membrane fusion between the virus and cell membranes. Expression of F and G in the same cell results in cell-cell fusion in transfected cell monolayers, while expression of F and G on their own in cell monolayers does not result in fusion. Co-culture of singly-transfected F and G cells also does not result in fusion. Expression of NiV G in transgenic CRFK cells results in resistance to NiV- and HeV-induced cytopathic effect. Additionally, neither NiV nor HeV nucleic acid could be detected in CRFK-NiV G that had been exposed to NiV or HeV. NiV G expression also prevents NiV F+NiV G-mediated cell-cell fusion, but does not affect cell surface expression of either virus receptor, ephrin-B2 and ephrin-B3. Chimeric glycoproteins derived from NiV G and CDV H were constructed and characterized. None of the chimeric glycoproteins were able to fuse when coexpressed with either NiV F or CDV F. Only one of the chimeric glycoproteins (H145/G458) was detected on the cell surface by immunofluorescence assay (IFA). None of the chimeric glycoproteins altered cell surface expression levels of ephrin-B2 and ephrin-B3. Finally, recombinant NiV genomes (rNiV and rNiV eGFPG) were constructed, as well as chimeric CDV genomes with NiV ORF substitutions (rCDV eGFPH NiVFG and rCDV eGFPH NiVMFG). The only chimeric virus that was generated, rCDV eGFPH NiVFG, was assessed for its release from infected cells. rCDV eGFPH NiVFG was poorly released from infected cells without a freeze-thaw cycle, but was also found to induce the cellsurface down-regulation of the viral receptors ephrin-B2 and ephrin-B3.

Nipah virus (NiV) and Hendra virus (HeV) have been identified as the causes of outbreaks of fatal meningitis, encephalitis, and respiratory disease in Australia, Malaysia, Bangladesh, and India from 1994 until 2004. In order to accommodate the unique genomic characteristics of NiV and HeV, a new genus within the family Paramyxoviridae was created, named Henipavirus. NiV encodes two surface glycoproteins: the attachment glycoprotein (G) binds to the cellular receptor for the virus, while the fusion glycoprotein (F) mediates membrane fusion between the virus and cell membranes. Expression of F and G in the same cell results in cell-cell fusion in transfected cell monolayers, while expression of F and G on their own in cell monolayers does not result in fusion. Co-culture of singly-transfected F and G cells also does not result in fusion. Expression of NiV G in transgenic CRFK cells results in resistance to NiV- and HeV-induced cytopathic effect. Additionally, neither NiV nor HeV nucleic acid could be detected in CRFK-NiV G that had been exposed to NiV or HeV. NiV G expression also prevents NiV F+NiV G-mediated cell-cell fusion, but does not affect cell surface expression of either virus receptor, ephrin-B2 and ephrin-B3. Chimeric glycoproteins derived from NiV G and CDV H were constructed and characterized. None of the chimeric glycoproteins were able to fuse when coexpressed with either NiV F or CDV F. Only one of the chimeric glycoproteins (H145/G458) was detected on the cell surface by immunofluorescence assay (IFA). None of the chimeric glycoproteins altered cell surface expression levels of ephrin-B2 and ephrin-B3. Finally, recombinant NiV genomes (rNiV and rNiV eGFPG) were constructed, as well as chimeric CDV genomes with NiV ORF substitutions (rCDV eGFPH NiVFG and rCDV eGFPH NiVMFG). The only chimeric virus that was generated, rCDV eGFPH NiVFG, was assessed for its release from infected cells. rCDV eGFPH NiVFG was poorly released from infected cells without a freeze-thaw cycle, but was also found to induce the cellsurface down-regulation of the viral receptors ephrin-B2 and ephrin-B3.

Orientador: Rogério Custodio / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Química / Made available in DSpace on 2018-08-23T02:21:16Z (GMT). No. of bitstreams: 1 Rocha_CarlosMuriloRomero_M.pdf: 3181887 bytes, checksum: 92221d4d84e390412b4bdcfb0ae7e206 (MD5) Previous issue date: 2013 / Resumo: No presente trabalho o pseudopotencial CEP foi implementado na teoria G3(MP2)B3 e a adaptação denominada G3CEP(MP2)B3. Tal método foi aplicado no estudo de 247 entalpias padrão de formação, 104 energias de ionização, 63 afinidades eletrônicas, 10 afinidades protônicas e 22 energias de atomização de um conjunto de moléculas contendo elementos representativos do 2º, 3º e 4º períodos da tabela periódica, totalizando 446 dados termoquímicos. Os desvios absolutos médios, em relação aos dados experimentais, foram 1,60 kcal mol e 1,41 kcal mol para as teorias G3CEP(MP2)B3 e G3(MP2)B3, respectivamente, com reduções de 10-40% nos tempos de CPU com a implementação do pseudopotencial CEP. Além disso, a avaliação de outras propriedades tais como cargas atômicas, momentos de dipolo e energias de orbitais HOMO resultou em desvios absolutos médios, em relação ao método G3(MP2)B3 original, de 0,203 e, 0,044 D e 0,002 Eh, respectivamente. Outro objetivo do presente trabalho foi a aplicação do método G3CEP(MP2)B3 no estudo do mecanismo de nitração do fenol, em fase gasosa, promovida pelo eletrófilo NO2 . Tal avaliação mecanística evidenciou-nos a ocorrência de transferências eletrônicas do sistema p aromático ao íon nitrônio em etapas que precedem a formação do complexo-s, resultados que são convergentes à hipótese do mecanismo SET (Single Electron Transfer). Além do mecanismo de substituição eletrofílica aromática, o presente estudo evidenciou a ocorrência, em fase gasosa, de caminhos reacionais alternativos, através dos quais a transferência da espécie O ao sistema p aromático do fenol seria observada. As excelentes concordâncias entre a teoria G3CEP(MP2)B3 e os demais métodos Gn mais acurados (como G3(MP2)B3, G3CEP e G3) na previsão de barreiras de ativação, revelou-nos interessantes perspectivas quanto à aplicabilidade da teoria G3CEP(MP2)B3 na determinação mecanística de reações orgânicas, bem como na previsão acurada de barreiras rotacionais internas, frente a reduzidos custos computacionais / Abstract: In this work, the CEP (Compact Effective Potential) pseudopotential was adapted in the G3(MP2)B3 theory providing a theoretical alternative referred to as G3CEP(MP2)B3 for calculations involving second-, third-, and fourth-row representative elements. The G3CEP(MP2) B3 theory was applied in the study of 247 standard enthalpies of formation, 104 ionization energies, 63 electron affinities, 20 proton affinities and 22 atomization energies of a test set comprising 446 experimental energies. The total mean absolute deviation was 1.60 kcal mol for G3CEP(MP2)B3 theory against 1.41 kcal mol from all-electron G3(MP2)B3 calculations, with reductions of 10-40% in CPU time for the implemented theory. Furthermore, the assessment of other properties such as atomic charges, dipole moments and highest occupied molecular orbital (HOMO) energies resulted in mean absolute deviations, compared with those predicted by the original G3(MP2)B3 theory, of 0.203 e, 0.044 D and 0.002 Eh, respectively. In addition to the adaptation and assessment of G3CEP(MP2)B3 theory, the purpose of this work was also the application of the implemented theory in the study of phenol nitration mechanism, in gaseous phase, promoted by NO2 elepctrophile. The mechanistic evaluation at G3CEP(MP2)B3 level showed the occurrence of a single-electron-transfer step from aromatic p-system to the nitronium ion prior to the s-complex formation, in agreement with the SET (Single Electron Transfer) mechanism. Besides electrophilic aromatic substitution reaction, the present work provided insights into alternative reaction mechanisms through which O species are transferred to the phenol aromatic p-system. Excellent agreement between G3CEP(MP2)B3 theory and other more accurate Gn theories (for instance G3(MP2)B3, G3 and G3CEP) in predicting activation barriers showed that the implemented theory would be a useful tool in the study of reaction mechanisms and also for predicting internal rotational barriers with a significantly reduced computational cost / Mestrado / Físico-Química / Mestre em Química

No description available.

Recent research demonstrated that besides a role in sex determination and male fertility, the Y chromosome is involved in additional functions including prostate cancer, sex-specific effects on the brain and behaviour, graft-versus-host disease, nociception, aggression and autoimmune diseases. The results presented in this thesis include an analysis of sex-biased genes encoded on the X and Y chromosomes of rodents. Expression data from six different somatic tissues was analyzed and we found that the X chromosome is enriched in female biased genes and depleted of male biased ones. The second study described copy number variation (CNV) patterns in a world-wide collection of human Y chromosome samples. Contrary to expectations, duplications and not deletions were the most frequent variations. We also discovered novel CNV patterns of which some were significantly overrepresented in specific haplogroups. A substantial part of the thesis focuses on analysis of spatial expression of two Y-encoded brain-specific genes, namely PCDH11Y and NLGN4Y. The perhaps most surprising discovery was the observation that X and Y transcripts of both gene pairs are mostly expressed in different cells in human spinal cord and medulla oblongata. Also, we detected spatial expression differences for the PCDH11X gene in spinal cord. The main focus of the spatial investigations was to uncover genetically coded sexual differences in expression during early development of human central nervous system (CNS). Also, investigations of the expression profiles for 13 X and Y homolog gene pairs in human CNS, adult brain, testes and still-born chimpanzee brain samples were included. Contrary to previous studies, we found only three X-encoded genes from the 13 X/Y homologous gene pairs studied that exhibit female-bias. We also describe six novel non-coding RNAs encoded in the human MSY, some of which are polyadenylated and with conserved expression in chimpanzee brain. The description of dimorphic cellular expression patterns of X- and Y-linked genes should boost the interest in the human specific gene PCDH11Y, and draw attention to other Y-encoded genes expressed in the brain during development. This may help to elucidate the role of the Y chromosome in sex differences during early CNS development in humans. / <p>chinese, finnish, norwegian, schizophrenia, bipolar, bipolar disorder, msy, male specific region Y, PAR1, PAR2, pseudoautosomal, male-biased, female-biased, male biased, female biased, ashkenazi population, structure, variants, YHRD, Elena Jazin, Björn Reinius, Per Ahlberg, brain, hjärna, CNS, central nervous system, IR2, inverted repeat 2, isodicentric, genetics, genetik, padlock, rolling circle, amplification, PCR, sY1191, sY1291, STS, DDX3Y, DAZ, AZFa, AZFb, AZFc, AZF, Repping, haplogroup J, rearrangements, DE-M145, I-M170, E-M96, Q-M242, R-M207, O-M175, G-M201, D-M174, C-M130, NO-M214, N-M231, poland</p>

Master's thesis deals with influence of various factors on the concrete compressive strength and modulus of elasticity. This work concentrates mainly on the influence of early curing and air entrainment of concrete. One part of this work is the evaluation of laboratory measurement, which was carried out on cubes of standard and norm prisms. The development of elastic moduli was monitored in time to 730 days of age with respect to air entrainment and curing conditions. The second part of the experiment deals with a comparison of theoretical creep curves depending on the value of the modulus of elasticity based on different standards. Again, the air entrainment effect and influence of initial conditions are reflected.