1 Abstract This thesis deals with a complete legal analysis of the process of the creation and the protection of the Natura 2000 network. The Natura 2000 system is a system of protected areas created on its territory by all EU Member States. Its aim is to protect the rarest and the most endangered species of wild plants, wildlife and natural habitat types occurring within the European Union. At the heart of the network there are two key legal acts of secondary legislation on nature protection, namely Directive 2009/147/EC of the European Parliament and of the Council on the conservation of wild birds and Council Directive 92/43/EEC on the conservation of natural habitats. The diploma thesis is structured into five voluminous chapters. In the first chapter, the author mentions international treaties which, with their subject-matter of protection, intertwine with the natura's directives and (not only) laid the foundation for their later admission. In the second chapter entitled Natura 2000, in the context of EU law, it draws attention to both the basic EU legal acts which gave rise to the Natura 2000 network but also to important soft law documents and the Regulation 1143/2014 of the European Parliament and of the Council on non- native invasive species whose interdependence with the said Directives is beyond...

Made available in DSpace on 2015-03-05T16:19:37Z (GMT). No. of bitstreams: 0 Previous issue date: 27 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Atividades de mineração causam alterações nas paisagens, acarretando mudanças na vegetação e nas propriedades e topografia do solo que acabam por afetar a fauna. Para minimizar os efeitos da mineração, empresas mineradoras freqüentemente implementam procedimentos de recuperação ambiental exigidos por lei. O presente estudo aborda o efeito e a influência da estrutura da vegetação nas comunidades de aves em áreas recuperadas após processos de mineração em florestas no município de São Mateus do Sul, sudeste do Estado do Paraná e em áreas de campo em Candiota no Rio Grande do Sul No Paraná selecionei quatro áreas para as amostragens: três delas, com 5, 10 e 20 anos de recuperação vegetal (A05, A10 e A20, respectivamente) encontram-se na Unidade da Petrobrás (Superintendência de Industrialização de Xisto) e a quarta área de estudo é um fragmento florestal que não sofreu interferências de mineração (A50). Em Candiota selecionei três áreas com vegetação nativas nunca antes mineradas e três áreas recuperadas. Nos do / Mining activities cause severe alterations in natural landscapes, changing the vegetation and the properties and topography of the soil, and thus altering habitats and the associated fauna. To minimize the effects of mining, mining companies implement procedures of environmental recuperation demanded by law. The present study investigates the influence of vegetation structure on bird communities in reclaimed areas after mining in forest and grassland habitats in south Brazil. Forested areas were studied in the State of Paraná, while grasslands were studied in the Pampa biome in Rio Grande do Sul. In Paraná I sampled four areas, three of them (with 5, 10 and 20 years of vegetation recuperation named, respectively, A05, A10, and A20), were located in the Unit of Petrobrás, and the fourth was a forest fragment that did not suffer mining interferences (A50). In Rio Grande do Sul I selected six areas, three with native vegetation never mined before and three reclaimed areas. I sampled the avifauna using the point

Orientador: Claudio Cabello / Banca: Edivaldo Antonio Garcia / Banca: Magali Leonel / Resumo: A produção de bioetanol utilizando raízes de mandioca irá produzir um resíduo lignocelulósico que juntamente com o vinho esgotado nas colunas de destilação, constituem-se nos principais resíduos gerados no processo. Normalmente os resíduos líquidos seguem para tratamento em lagoas de estabilização enquanto que os resíduos sólidos são descartados no ambiente incorporando esta biomassa ao solo. A fim de se obter um melhor rendimento econômico na produção de etanol a partir das raízes de mandioca, o presente trabalho teve como objetivo realizar a caracterização físico-química de resíduos sólidos gerados em dois tipos de processamento da matéria prima numa planta de fabricação de etanol e, verificar a possibilidade de sua inclusão em dietas formuladas para frangos de corte aproveitando os nutrientes remanescentes. O processamento das raízes de mandioca teve inicio com a lavagem e desintegração das raízes com adição de 20% de água até obter uma polpa que foi tratada em reator agitado adicionando enzima -amilase e temperatura de 90°C por 2 horas. Em seguida, ajuste de pH, abaixamento da temperatura para 60°C e adição de enzima amiloglucosidase com agitação por 14 horas para ser efetuada a fermentação. O hidrolisado obtido foi a fonte dos dois tipos de resíduo quais sejam: i) resíduo obtido da filtração do hidrolisado antes da fermentação e; ii) resíduo obtido da filtração do vinho alcoólico após fermentação do hidrolisado... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The elaboration of bioethanol using cassava roots, produces a lignocellulosic waste which along with the wine produced in the distillation columns, constitute the main kind of waste generated in the process. The liquid waste is treated in stabilization ponds, while the solid waste is disposed in the environment, incorporating such biomass to the soil. Aiming at to get the best advantage from the production of ethanol, from cassava roots, this work presented a physical-chemical characterization from two different kinds of waste obtained from two different kinds of such raw material processing, and developed a methodology for including this into fattening chicken diets in the early phase, getting the best possible from the carbohydrates. The processing of cassava roots began with the disintegration and washing the roots with the addition of 20% of water to obtain a pulp which was treated in stirred reactor adding enzyme -amylase and temperature of 90 ° C for 2 hours. Then, adjust the pH, lowering the temperature to 60 ° C and addition of enzyme amiloglucosidase and stirring for 14 hours. The hydrolyzate obtained was the source of two types of waste which are: i) type 1 solid residue obtained after filtration of the hydrolyzate and ii) solid waste type 2 obtained from filtering the wine after alcoholic fermentation of the hydrolyzate. The experimental design included both types of waste at levels of 5, 10 and 15% in diets for broiler chickens for a period of 21 days. The experiments showed that the inclusion of hydrolyzed cassava waste in the formulation of diets adversely affected the performance of the animals, while the inclusion of fermented residue doesn't affected the performance in experiments. / Mestre

Orientador: Marco Aurélio Pizo Ferreira / Banca: Alexsander Zamorano Antunes / Banca: Augusto João Piratelli / Banca: José Ragusa Netto / Banca: Mauro Galetti Rodrigues / Resumo: A expansão de plantações florestais tem sido intensificada nos últimos anos, ultrapassando 70.000.000 ha nos trópicos. Eucalyptus é o principal gênero plantado, ocupando 50% das áreas silviculturais. Monoculturas arbóreas e fragmentos de vegetação secundária figurarão entre os principais elementos das paisagens tropicais futuras. Logo, compreender como diversas espécies se comportam diante dessa situação é fundamental quando se objetiva a conservação da biodiversidade. Este trabalho estudou a avifauna em um mosaico composto por talhões de Eucalyptus spp. e alguns de vegetação nativa na Floresta Estadual Edmundo Navarro de Andrade (Rio Claro, SP). Esses talhões possuíam diferentes idades (plantados de 1934 a 1990), extensões (~1-51 ha) e graus de regeneração de vegetação nativa. Os principais objetivos foram caracterizar a avifauna encontrada nesses talhões quanto à riqueza, abundância e composição e compará-las, verificando possíveis associações com a estrutura da vegetação (riqueza, altura, área basal, densidade de plantas, densidade de plantas mortas, cobertura do dossel e densidade vertical nos estratos de 0,0 a 1,0, de 1,1 a 2,0 e de 2,1 a 5,0 m). Para a amostragem de aves foi empregado o método de pontos. No primeiro capítulo, foram amostrados 14 talhões de E. citriodora. Foram registradas 103 espécies de aves, variando de 23 a 55 por talhão. Myiodynastes maculatus, Cyclarhis gujanensis, Amazilia lactea e Megarynchus pitangua mostraram maior plasticidade ambiental, não revelando fortes associações com as variáveis da vegetação. A abundância total foi maior em talhões que apresentavam vegetação mais desenvolvida. Talhões com maior complexidade da vegetação apresentaram maior riqueza e abundância de espécies insetívoras e de dependentes de florestas. A distância entre os talhões de E. citriodora e de vegetação nativa... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The expansion of planted forests is surprisingly fast in the last years, exceeding 70.000.000 ha in the tropics, 50% of which are composed of Eucalyptus spp.. Planted and secondary forests are likely to be dominant features of tropical forest landscapes in the future. Therefore, to understand the use of different species in this situation in basic to the biological conservation. This work investigated the avifauna at a mosaic of Eucalyptus spp. patches in the State Forest Edmundo Navarro de Andrade (Rio Claro, Sâo Paulo, Brazil). These patches (~1-51 ha) were created in different years (1994-1990), presenting different levels of regeneration of the native vegetation. The principal aims were to evaluate the richness, abundance and composition of the bird community associating these parameters to the structure of the vegetation (richness, height, basal area, plant density, dead plant density, canopy cover and vertical density from 0,0 to 1,0, from 1,1 to 2,0 and form 2,1 to 5,0 m). The avifauna was sampled with point counts. In the first chapter, 14 patches of E. citrioddora were studied. It was recorded 103 species, from 23 to 55 per patch. Myiodynastes maculatus, Cyclarhis gujanensis, Amazilia lactea and Megarynchus pitangua were the most habitat generalist species. These species were not associated to any vegetation parameter. The abundance was greater in patches with more developed vegetation. Patches with high vegetation complexity had greater richness and abundance of insectivorous and forest dependent species. The distance between E. citriodora and native vegetation patches influenced negatively the richness and abundance of forest dependent species. In chapter 2, in addition to the 14 E. citriodora patches, four E. microcorys and three native vegetation patches were included in the analyses. It was recorded 115 species. On average, there were 47, 35 and 24 species in the patches... (Complete abstract click electronic access below) / Doutor

Orientador: Marcos César de Oliveira Santos / Banca: Marco Aurélio Pizo Ferreira / Banca: Olavo Nardy / Resumo: A choca-do-planalto (Thamnophilus pelzelni Hellmayr, 1924) é um Thamnophilidae insetívoro que ocorre da área central do Brasil até o Nordeste. É considerada uma espécie comum e não está ameaçada de extinção. Informações sobre aspectos da área de vida são escassas, e assim torna-se necessário investir em esforços de pesquisas. O objetivo deste estudo foi estimar o tamanho da área de vida da choca-do-planalto e comparar o tamanho da área de vida entre o período chuvoso não reprodutivo, período seco não reprodutivo e período chuvoso reprodutivo. O estudo foi realizado dentro de uma grade de 30 ha na Estação Ecológica de Jataí (21°30' - 21°40'S e 47°40' - 47°50'W), município de Luiz Antônio, SP, entre fevereiro de 2009 e janeiro de 2010. Para a demarcação das áreas de vida, os indivíduos de T. pelzelni foram capturados com redes de neblina e marcados com uma anilha padrão CEMAVE e duas anilhas coloridas. As trilhas da grade eram percorridas e os indivíduos detectados tinham sua sequência de anilhas identificada com a ajuda de um binóculo (10x50) e a localização era determinada com ajuda de um GPS. Para estimar a área de vida foi utilizado o Minimo Poligono Convexo (MPC) com probabilidade de 100% e o Kernel Fixo (KF) 95% e 50% referentes, respectivamente, a área de vida e área core. Para comparar o tamanho da área de vida nos três períodos amostrados foi feito análise de variância para medidas repetidas (RM-ANOVA) e o teste de Tukey foi usado para identificar qual média diferiu significativamente entre si. O tamanho da área de vida calculada pelo MPC variou de 2,7 a 4,8 ha, com média de 3,3 ± 0,6 ha Pelo estimador de KF 95% a área de vida variou de 3,4 a 7,0 ha, com média de 4,7 ±1,1 ha. A área core (KF 50%) apresentou média de 1,1 ± 0,3 ha. Os tamanhos das áreas de vida variaram significativamente entre os três períodos... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The Planalto Slaty-Antshrike (Thamnophilus pelzelni Hellmayr, 1924) is an insectivore Thamnophilidae that occurs in the central area of Brazil to the northeast of the country. It is a common species and considered not threatened. Information about aspects of home range is scarce. The aim of this study was to estimate the home range of Planalto Slaty-Antshrike and compare the extent of home range among the non-reproductive rainy season, non-reproductive dry season and breeding rainy season. Data collection occurred in a grid of 30 ha in Jataí Ecological Station (21 ° 30 '- 21 ° 40'S and 47 ° 40' - 47 ° 50'W), municipality of Luiz Antônio, SP, between February 2009 and January 2010. Individuals of T. pelzelni were captured with mist nets and banded with a numbered aluminum leg band (pattern CEMAVE) and with two colored bands. Each track of the grid was traversed and in each contact, individuals detected had their sequence of washers identified with the aid of binoculars (10x50) and the location determined with the use of a GPS. The Minimum Convex Polygon (MCP) was applied with a probability of 100% and Fixed Kernel (FK) 95% and 50% concernig, respectively, the home range and core area. To compare the extent of home range in three sampling periods, analysis of variance was performed for repeated measures (RM-ANOVA), and Tukey test to identify which average differ significantly. The size of home range calculated by the MCP ranged from 2.7 to 4.8 hectares, with an average of 3.3 ± 0.6 ha. To apply the FK estimator at 95% of probability, the home range ranged from 3.4 to 7.0 ha, with a rough average of 4.7 ± 1.1 ha. The core area (FK 50%) resulted 1.1 ± 0.3 ha. The extent of the home ranges altered significantly between three sampling periods (MCP ˂ P 0.001; FK 95% P ˂ 0,001, 50% FK ˂ P 0.001). Tukey test exhibited that the home ranges were significantly larger during the dry... (Complete abstract click electronic access below) / Mestre

For migrant birds, the non-breeding season can greatly influence survival and future reproductive success. Knowledge of annual and overwinter survival, the degree of site fidelity and habitat use in the non-breeding season, migration ecology, routes and stopovers, and whether these differ with age or sex is fundamental to understanding population dynamics, vulnerability to anthropogenic habitat degradation, and consequently for understanding the severe widespread declines of migrant bird species. The degree to which a migrant is a winter specialist or generalist is likely to be central to understanding population dynamics. I studied survival rates and the wintering and migration ecology of a declining Palearctic migrant, the whinchat Saxicola rubetra, wintering in West Africa, to establish how the non-breeding season may influence migrant population dynamics. Whinchats were extremely site faithful to both within and between years, holding distinct winter territories and returning to those territories in subsequent winters, despite the opportunity to relocate. Overwinter survival was very high and annual survival was comparable to or higher than that reported on the breeding grounds. Because our power to detect resident and dispersing birds was high, survival rates likely estimated true survival well. Habitat characteristics varied widely across territories and territories were smaller if more perching shrubs and maize were present. Most individuals showed a tolerance or even preference for human modified habitats. Some individuals may have multiple wintering sites. There was no evidence of dominance-based habitat occupancy or any differences in winter ecology, site fidelity, survival and most aspects of migratory behaviour between age and sex classes. Migratory connectivity occurred only on a large-scale and individual migratory behaviour was also varied. Fundamentally, the results suggest a generalist strategy in the non-breeding season within their wintering habitat of open savannah, most likely as an adaption to stochastic site selection within the wintering range for juveniles undertaking their first migration plus changing and unpredictable conditions both within and between years. Consequently, wintering conditions may not significantly limit whinchat populations and mortality is probably highest during active migration. Notably, non-specialist migrants such as whinchats may have some resilience at the population-level to the increasing anthropogenic habitat modification occurring in Africa, suggesting that conditions during migration and in Europe may be driving declines; yet establishing the currently unknown thresholds of any resilience is likely to be fundamental for the future conservation migrants.

Farmland bird populations in Europe have been in decline for a long time. Agricultural intensification and growing a large share of crops that provide suboptimal breeding habitat could be the main causes of the observed decline. To explore these possible drivers, I focused on population trends of farmland bird species in different habitats in the Czech Republic over the periods 1982-1990 and 1990-2010. Specifically, I focused on the variability in trends within each species in respect to the differences in landscape management between the lowlands and mid-altitude areas before and after 1990. The expected effect of intensive farming in the lowlands until 1990 was reflected by a strong decline in populations of Northern Lapwing and Yellowhammer. The influence of less intensive farming in mid-altitude areas causing moderate population decline was confirmed rather by shrubs and trees species than farmland specialists. After 1990, less intensive farming in the lowlands should reduce the decline, which may have occurred, but compelling comparison of population trends between the two time periods was not possible for most species due to the lack of data. On the contrary, more intensive population decline in mid-altitudes after 1990, which should be the response to arable land abandonment, was not...

Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico / As doenÃas cardiovasculares (CVDs) compreendem um amplo espectro de doenÃas do coraÃÃo e vasos sanguÃneos (artÃrias e veias), entre as quais se incluem a doenÃa das artÃrias coronÃrias, o ataque cardÃaco, a angina, a sÃndrome coronariana aguda, o aneurisma da aorta, arritmias cardÃacas, a doenÃa cardÃaca congÃnita, a insuficiÃncia cardÃaca e a doenÃa cardÃaca reumÃtica. Entre os principias fÃrmacos que tratam as doenÃas cardiovasculares estÃo: (i) as estatinas, que atuam inibindo a 3-hidroxi-3-metilgluratil coenzima A (HMG-CoA) redutase no processo de conversÃo da HMG-CoA em mevalonato, numa das etapas da biossÃntese do colesterol. Observa-se em ensaios clÃnicos que a aÃÃo das estatinas pode diminuir os nÃveis de colesterol de baixa densidade (LDL) entre 20\% e 60\%, reduzindo os eventos coronarianos em atà 1/3 no perÃodo de cinco anos; (ii) a aspirina, com a qual hà mais de 400 preparaÃÃes nos EUA e se produz cerca de 20 mil toneladas anualmente. ApÃs mais de um sÃculo de prÃtica clÃnica, a aspirina continua sendo a droga antitrombÃtica, antitÃrmica, analgÃsica e antiproliferativa mais amplamente recomendada. Ela age bloqueando a biossÃntese de hormÃnios inflamatÃrios prostanÃides atravÃs da inibiÃÃo das enzimas ciclooxigenase COX-1 e COX-2; (iii) os anti-hipertensivos, para os quais a Enzima Conversora de Angiotensina (ECA) à o principal alvo (inibidores da ECA estÃo no mercado a mais de 20 anos) visando o combate das pressÃes arteriais elevadas, que provocam alteraÃÃes nos vasos sanguÃneos e na musculatura do coraÃÃo, e levam a hipertrofia do ventrÃculo esquerdo do coraÃÃo, acidente vascular cerebral, infarto do miocÃrdio, morte sÃbita, insuficiÃncias renal e cardÃaca, etc. A hipertensÃo arterial (HTA) ou hipertensÃo arterial sistÃmica (HAS), conhecida popularmente como pressÃo alta, à uma das doenÃas com maior prevalÃncia no mundo moderno. A ECA atua na regulaÃÃo da pressÃo sanguÃnea via conversÃo do decapeptÃdeo angiotensina I no potente vasopressor angiotensina II e tambÃm pela inativaÃÃo da bradicinina, sendo componente central do Sistema Renina-Angiotensina-Aldeosterona (SRAA), que controla a pressÃo sanguÃnea e tem forte influÃncia nas funÃÃes relacionadas ao coraÃÃo e os rins, bem como na contraÃÃo dos vasos sanguÃneos. Nesta tese realiza-se um estudo da bioquÃmica quÃntica de estatinas (atorvastina, rosuvastatina, cerivastatina, mevastatina, sinvastatina e fluvastatina), da aspirina/bromoaspirina e de anti-hipertensivos (captopril, enalapril, lisinopril, ramipril, trandolapril e perindopril) levando-se em conta dados cristalogrÃficos dos seus sÃtios de ligaÃÃo nas proteÃnas HMGR, COX-1 (o da aspirina foi simulado partindo-se dos dados da bromoaspirina) e ECA, respectivamente. As simulaÃÃes computacionais foram realizadas considerando-se a Teoria do Funcional de Densidade (DFT) na aproximaÃÃo da densidade local (LDA) e funcional de troca e correlaÃÃo PWC, com energia de interaÃÃo entre os resÃduos das proteÃnas circunscritos ao sÃtio de ligaÃÃo de raio r e os fÃrmacos calculada atravÃs do mÃtodo de fracionamento molecular com capas conjugadas (MFCC). Os resultados obtidos para as estatinas sugerem que: (i) as mais (menos) eficazes sÃo a atorvastatina e a rosuvastatina (sinvastatina e fluvastatina), o que està de acordo com a clÃnica e valores dos seus Ãndices de concentraÃÃes inibitÃrias IC50; (ii) sÃtios de ligaÃÃo com raios de pelo menos 12 à (alÃm do raio de 9,5 à sugerido pela anÃlise estrita de dados cristalogrÃficos) devem ser considerados para que resÃduos importantes como E665, D767, e R702 sejam considerados para que as eficiÃncias das estatinas sejam corretamente explicadas. Para a aspirina/bromoaspirina utilizou-se um refinamento quÃntico de segunda ordem dos dados cristalogrÃficos para se demonstrar que a energia de ligaÃÃo de ambos com a COX-1 sÃo aproximadamente a mesma, o que explica resultados experimentais de IC50 similares. A existÃncia de resÃduos atrativos e resulsivos à destacada, mostrando-se que Arg120 à o resÃduo que mais atrai o Ãcido salicÃlico apÃs acetilaÃÃo da Ser530, seguido de Ala527, Leu531, Leu359 e Ser353; por outro lado, Glu524 à o resÃduo repulsivo mais efetivo (intensidade comparÃvel ao Arg120), nunca tendo sido antes considerado como resÃduo importante no sÃtio de ligaÃÃo da aspirina/bromoaspirina na COX-1. Finalmente, no caso dos anti-hipertensivos, obtÃm-se que à necessÃrio se considerar raios do sÃtio de ligaÃÃo de 16 à para se obter que o lisinopropil e o ramipril (trandolapril e perindopril) apresentam as maiores (menores) energias de ligaÃÃo, o que explica a maior (menor) constante de inibiÃÃo dos mesmos entre os anti-hipertensivos estudados para a ACE da Drosophila melanogaster.

The Corn Bunting Emberiza calandra is one of the most severely declining farmland birds across Europe. In the UK, numbers fell by 86% between 1967 and 2008. Corn Buntings favour open landscapes, nest on or close to the ground, are often polygynous, double-brooded, and have a seed-based diet supplemented in summer by invertebrates. This study investigated the recent causes of decline in arable and mixed farmland in eastern Scotland, and sought to identify potential conservation solutions that could be delivered through agri-environment schemes (AES). Combining new data with analyses of existing long-term datasets, I investigated habitat associations during summer and winter, the timing and success of nesting attempts, and measured reproductive and population responses to AES. Corn Buntings declined almost to extinction in one study area where, over 20 years, the main recorded intensifications of farming were reduced weed abundance within crops and removal of boundaries to make bigger fields. Territory locations, late-summer occupancy and polygyny were all strongly associated with weedy fields. There were also positive associations with overhead wires and in early summer with winter barley and forage grasses. Late-summer occupancy was associated with spring-sown cereals, crops that are amongst the last to be harvested. Changes in habitat associations and to aspects of the mating system as the population declined and agriculture intensified are discussed. Intensive monitoring showed that Corn Buntings laid clutches from mid-May to mid-August, mostly in fields of forage grasses and autumn-sown cereals in early summer, and spring sown cereals in late summer. A preference for nesting in dense swards explained this seasonal variation. Breeding success in forage grasses was poor, due to high rates of nest loss during mowing. However, in experimental trials, nest success in fields with delayed mowing was fivefold that of control fields. With sufficient uptake through AES, delayed mowing could raise productivity to levels required to reverse population declines. In winter, cereal stubbles and AES unharvested crop patches were the main foraging habitats used. Unharvested crops with abundant cereal grain in their first winter of establishment were favoured. Population monitoring over seven years and 71 farms revealed increases on farms with AES targeted at Corn Buntings, no significant change on farms with general AES, and declines on control farms. In arable-dominated farmland, management that increased food availability reversed declines, but on mixed farmland where Corn Buntings nested in forage grasses, delayed mowing was essential for population increase. This study has already influenced the design of AES targeted at Corn Buntings in Scotland, and I make further recommendations for the species’ conservation and design of AES that are applicable to farmland throughout Britain and Europe.

A prática popular de manutenção de aves como animais de estimação é um importante fator de risco à saúde pública. Não obstante o benefício gerado pelo convívio com o animal, as aves também podem albergar agentes de doenças transmissíveis para seres humanos. A Chlamydophila psittaci, ocupa uma posição de destaque dentre as zoonoses de origem aviária. O presente estudo, que teve como objetivo, em uma primeira etapa, determinar o nível de conhecimento dos proprietários de aves sobre manejo e zoonoses. No segundo momento, calopsitas (Nymphicus hollandicus), naturalmente infectadas com C. psittaci foram submetidas a um protocolo de tratamento com antibiótico durante quarenta e cinco dias consecutivos. Foram efetuadas biopsias hepáticas e swabs de cloaca, submetidos à técnica de PCR e cultura bacteriológica, para avaliar o impacto do tratamento no organismo do animal, microbióta bacteriana e o estado do portador. Concluiu-se que os proprietários precisam de uma orientação mais contundente e direcionada à prevenção e manejo de suas aves; o protocolo de tratamento utilizando a doxiciclina foi eficaz, eliminando a positividade das aves; as avaliações observadas nas biópsias hepáticas não mostraram alterações significativas que pudessem contribuir com o diagnóstico da clamidiose / The maintenance of pet birds in home cages is a risk to public heath, this animals can house important zoonotic agents like Chlamydophila psittaci, that causes avian clamydiosis. The first aim of this study was to measure the clamydiosis knowledge level of bird owners that had their animals treated in a private veterinary clinic in São Paulo trough a questionnaire with semi-opened questions about behaviour, handling and cleaning of their pets. The second aim of this report was to evaluate the doxiciclin treatment eficacy in naturally infected cockatiels (Nymphicus hollandicus) with oral aplications of 25-35mg doxicicin/kg/day during 45 consecutive days. Every bird has had two liver biopsies done: the first before the treatment and the second one after that. During the treatment severam cloacal swabs were performed to evaluate the treatment impact in the digestive microbiota trough PCR and bacterial culture. The present study concludes that the bird owners need to be better informed about handling and disease prevention. Besides, the doxiciclin treatment was effective when evaluated trough the histopathologic evaluation (liver biopsies), making the positive samples turn to negative after 45 days. The liver collection was concedered a safe procedure, not causing mortality or complications after it and the samples were representative to the determined diagnosis. The liver evaluation could not show any significant variation that would help in the clamidiosis diagnose