Le paludisme ou malaria est une maladie parasitaire due à un protozoaire. Il est transmis principalement à l'homme par la piqûre de la femelle d'un moustique du groupe des anophèles. La transfusion sanguine représente la 3e voie potentielle de transmission de Plasmodium. L'incidence du paludisme a entraîné une augmentation de la proportion des donneurs de sang ayant été en contact avec le parasite. Le premier objectif de ce travail de thèse est d'analyser l'immune-réactivité de marqueurs biologiques de dépistage de Plasmodium dans les poches de sang pour prévenir le paludisme transfusionnel et plus spécifiquement, il s'agit de déterminer par goutte épaisse (GE) et Enzyme Linked lmmunosorbent Assay {ELISA), la prévalence du paludisme asymptomatique chez les donneurs de sang au Sud du Bénin. Le second objectif est de répertorier les marqueurs moléculaires les plus adéquats pour la recherche d'antigène et d'anticorps de Plasmodium, utilisables en dépistage de masse dans les centres de transfusion sanguine. / Malaria is a disease due a protozoan. lt is transmitted to humans by the bite of a mosquito form Anopheles group. Blood transfusion is the third potential way of malaria transmission. Incidence of Malaria has increased the proportion of blood donors suspected to be contaminated by Plasmodium sp. The goal of that study is to determine the immunoreactivity of some biomarkers that could be used for the prevention of the blood transfusion transmitted malaria. For that purpose we used thick and thin blood film microscopical determination and an Enzyme Linked lmmunoSorbent Assay technology detecting malaria antigen (pan-pLDH) and malaria antibodies. These methods were used for the screening of 2515 blood donors during ten following months insouthern-Benin, sample were separated in the 4 following seasons observed in Western Africa.

Les Encéphalopathies Spongiformes transmissibles (EST) sont des maladies neurodégénératives fatales caractérisées par l’accumulation d’un conformère anormal (PrPSc) d’une protéine de l’hôte (PrP). Chez l’homme, plusieurs centaines de cas de transmissions iatrogènes de la maladie de Creutzfeldt Jakob (MCJ) ont été identifiées, notamment chez des patients ayant fait l’objetd’ une greffe de dure-mère, de cornée ou des injections d’hormones de croissance extractives. Plus récemment, plusieurs cas du variant de la maladie de Creutzfeldt Jakob (vMCJ) ont été observés chez des patients transfusés avec des produits sanguins issus de donneurs en incubation de la maladie. D’un point de vue sanitaire, l’évaluation du risque de contamination interindividuelle par des tissus ou des fluides biologiques issus de patients atteints représente un enjeu important en matière de santé publique. La première partie de notre travail a consisté à comparer la sensibilité relative de modèles de souris transgéniques sur-exprimant la PrP à celle de l’hôte conventionnel exprimant la même séquence. Les résultats obtenus ont validé le concept d’une absence d’impact du niveau d’expression de la PrP ou du fond génétique de l’hôte sur la sensibilité finale du modèle à l’infection. A l’aide de ces modèles de souris transgéniques, nous avons alors mesuré les niveaux d’infectiosité dans le sang de patients atteints de différentes formes d’’EST. Chez un patient atteint de vMCJ, nous avons mis en évidence de faibles niveaux d’infectiosité dans les concentrés de globules rouges, les leucocytes et le plasma. Nous avons également pu détecter de l’infectiosité dans le plasma issu de 2 patients atteints de sMCJ sur 4 testés. Parallèlement à ces expériences, nous avons démontré dans un modèle expérimental d’infection chez le mouton, que l’administration de 104 à 105 leucocytes suffisent à transmettre par voire transfusionnelle la maladie. Ces résultats soulignent l’intérêt et les limites de la leuco-déplétion appliquée de manière standard en médicine transfusionnelle, pour limiter les risques de transmission du vMCJ. Enfin, nous avons testé la capacité de différents outils in vitro à détecter la présence des Prions dans le sang. / Transmissible spongiform encephalopathies (TSE) are fatal neurodegenerative disorders occurring in a wide spectrum of animals. They are characterized by accumulation of abnormally folded conformers (PrPSc) derived from normal cellular PrP protein (PrP) of the host. In human, many iatrogenic transmissions of Creutzfeldt Jakob disease (CJD) have been reported after dura mater graft, corneal graft or extractive growth hormone injections, prepared from affected donors. More recently, several cases of vCJD transmissions were reported in individuals that were transfused with blood from asymptomatic donors that subsequently developed vCJD. Risk assessment of interindividual transmission with contaminated tissues or body fluids remains a major public health issue. In a first part, we validated the final pertinence of infectious titers as measured in mice overexpressing PrP to the risk of transmitting the disease in the natural host species. In a second time, we used this model to evaluate the presence of infectivity in blood from TSE affected patients. We were able to detect the presence of infectivity in erythrocytes, leukocytes, and plasma of 1 person with vCJD and in the plasma of 2 out of 4 persons whose tests were positive for sporadic CJD. We then demonstrated in a sheep TSE model, that intravenous administration of 104 to 105 leucocytes was sufficient to cause disease in recipient sheep, underlying the efficacy and potential limits of leuko-reduction processes that are currently applied in transfusion medicine to mitigate the TSE transmission risk. Finally, using the same model, we tested different in vitro methods to detect prions in blood.

The dissertation takes the form of an impact study. It is based on a ten month period of research involving a literature review, interviews with headmasters of secondary schools who gave permission to survey their students, educational talks to secondary school learners and a survey of 500 learners from different schools in the Mthatha region. Purpose: The research endeavour addressed the problem of investigating the effectiveness of blood donor education in secondary schools in Mthatha region. Design/methodology/approach: This study employed a questionnaire-based survey. Five schools namely Mthatha High School, Holy Cross High School, Strategic High School; St Johns’ College and Zamukulungisa High School participated in this study. The Statistical Analysis System was employed to assess the association between the awareness strategies and general donor response. A chi–square test of association, based on the p –value approach, was used to achieve the results. Findings: This study finds that a blood donor education programme can increase blood donation awareness; benefit the schools and community, thus increasing the blood donor base. Receiving information as to the criteria for blood donation and how each individual donation is used to save a life is seen as a powerful means of reinforcement. Research limitations/implications: Further research is recommended on a large scale, involving all schools in Mthatha region. The research was delayed because it employed a new approach and a team had to be appointed before the blood donor centre can be opened. It is further recommended a once–off educational programme is not ideal because learners may not have received blood donation education before and should be repeated in follow-up sessions. Originality: This is the first research study looking at the effectiveness of a blood donor education programme in Mthatha secondary schools.

Orientador: Egle Novaes Teixeira / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia Civil, Arquitetura e Urbanismo / Made available in DSpace on 2018-08-26T13:52:27Z (GMT). No. of bitstreams: 1 Vicente_ClaudiaSpegiorin_D.pdf: 5969713 bytes, checksum: ed0a90aaf19195b916ebd3398b2868c0 (MD5) Previous issue date: 2014 / Resumo: Os hemocomponentes, concentrados de hemácias, concentrados de plaquetas e plasma, são os produtos obtidos a partir do sangue total doado pelo doador de sangue. São os principais produtos de um serviço de hemoterapia, e devem ter características que garantam a segurança da terapia transfusional. No acompanhamento da cadeia geradora de resíduo do Hemocentro de Campinas, o descarte de unidades de sangue total e de hemocomponentes chama a atenção. A Metodologia de Produção mais Limpa (P+L) é uma ferramenta interessante para tratar as causas destes eventos, pois, através dela, é possível analisar as causas de geração deste resíduo, promover a minimização e a busca por soluções que tragam benefícios sociais, econômicos e ambientais. Neste estudo foi analisado o fluxo das bolsas plásticas para coleta de sangue de doadores desde a aquisição até a finalização da transfusão, tendo como referência a metodologia da P+L para identificar as causas e as possibilidades de minimização do descarte de unidades sangue total e de hemocomponentes em cada processo. Foram identificadas seis oportunidades de P+L, uma no processo de Gestão de Materiais, três no processo de Coleta de Sangue Total, duas no processo de Processamento, e uma no processo do Laboratório de Compatibilidade. Os respectivos planos de monitoramento definidos incluíram ações de gerenciamento dos processos de trabalho, mudança de técnica e mudança de material. As principais barreiras identificadas estão relacionadas aos recursos econômicos, ao dimensionamento de recursos humanos, e ao planejamento de ações de captação de doadores e coleta de sangue total sem considerar a capacidade operacional da unidade de processamento. Na análise do lay out da área física e do fluxo de atividades da sala de coleta identificou-se pontos críticos que induzem ao erro e podem gerar o descarte. Conclui-se que a aplicação da Metodologia P+L possibilita a identificação das causas de descarte e das quantidades descartadas, as ações propostas podem promover a minimização da geração deste resíduo e no acompanhamento das ações propostas e dos resultados, definidos nos planos de monitoramento para cada oportunidade, cria-se um ciclo ininterrupto da aplicação contínua de uma estratégia preventiva de geração de resíduo, tal qual preconizada na definição de P+L feita pela UNIDO / Abstract: Blood components, packed red cells, platelet concentrates and plasma, are the products obtained from whole blood donated by blood donors. These are the main products of a transfusion service, and must have features that ensure the safety of transfusion therapy. In monitoring the chain of waste generated by the Blood Center of Campinas, the disposal of units of whole blood and blood components draws attention. The Methodology of Cleaner Production (CP) is an interesting tool for addressing the causes of these events, enabling the analysis of the causes of this waste generation, promoting the minimization of this waste and for seeking solutions that bring social, economic and environmental benefits. In this study the flow of plastic bags for blood donors was analyzed; from acquisition to completion of transfusion, using the P + L methodology as the reference to identify the causes of the disposal of units of whole blood and blood products and possibilities for minimizing waste discard in each process. Six opportunities of CP were identified, one in the process of Material Management, three in the process of Whole Blood Collection, two in the process of blood processing, and one in the Compatibility Laboratory process. Monitoring plans included management actions, defined work processes, technical changes and material changes. The main barriers identified were related to economic resources, design of human resources, donor recruitment planning and collection of whole blood without considering the operational capacity of the processing unit. During the analysis of the physical layout of the area and the flow of activities in the collection room, critical points inducing error and generating waste were identified. We concluded that the application of the CP methodology enabled the identification of the causes of waste and of the quantities discarded, moreover the actions proposed could promote minimization of waste generation and that the monitoring of proposed actions and outcomes, defined in the monitoring plans at every opportunity, created a continuous cycle of application of preventive strategies for waste generation, as recommended by UNIDO / Doutorado / Saneamento e Ambiente / Doutora em Engenharia Civil

Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Bc. Kateřina Nováková Supervisor: RNDr. Gabriela Červená, Ph.D. plk. MUDr. Miloš Bohoněk, Ph.D. Title of diploma thesis: Whole blood - New Blood Component Containing Platelets Background: The aim of this thesis was to evaluate in vitro quality parameters and haemostatic function of leucodepleted WB and their comparison with non-leucodepleted WB. The same way the quality parameters (RBC, PLT, HB and hemolysis) of erythrocyte blood component were measured and compared in day D14, D21, D35 and D42. Methods: WB collected from 30 healthy group A donors was divided into two groups - leucodepleted (LWB), using in-line platelet-sparing filters and collected to a blood bag system IMUFLEX® WB-SP (Terumo BCT, USA) and non-leucodepleted WB (NLWB), colletected to a blood bag system CompoFlex® Single System (Fresenius Kabi, Germany). Both groups were stored at 42řC for 14 days and following parameters were measured in days D0, D1, D3, D5, D10 and D14: WBC, RBC, PLT, HB, hemolysis, pH, TEG, FVIII, TT, PT, aPTT, aggregometry, concentration of PF4 and sCD40L (ELISA). Moreover, in days D0, D7 and D14 was measured the level of expression of platelet activation marker CD62P (P-selectin), CD42b, CD61 by...

No presenta resumen. / Revisión por pares

No presenta presenta resumen. / Revisión por pares

Title of Diploma Thesis: Production and use of transfusion products in the District Hospital Trutnov. Aim of the work: The aim was to evaluate the production of transfusion products (TP) in the period 2014-2019 at the Transfusion and Hematology Department (THO) in the District Hospital Trutnov and their use for hemotherapy by individual medical disciplines. Futhermore, to compare the production of TP within the Czech Republic together with selected European countries. The incidence of post-transfusion reactions and other adverse events during the reporting period is also included. Material and methods: The methodological part describes the standard procedures of pre- transfusion examination using the column agglutination method from the company Grifols, S.A., laboratory examination of donors and transfusion products manufactured at the Transfusion Department Trutnov. Conclusion: Despite all the recommendations and efforts to reduce the use of transfusion products, this condition has not been proven both in our department, within the Czech Republic and in Europe. This work recommends deleucotization of transfusion products for the national and European part, unification of procedures for transfusion in a given disease, mandatory introduction of NAT testing to minimize the risk of transmission...

Thesis (M. Tech.) -- Central University of Technology, Free State, 2008 / Blood transfusions carry a number of risks, one of which is transmitting HIV/AIDS from an infected donor. Since HIV is sexually and parenterally transmitted, the initial HIV risk management of donated blood in the early 1980‟s consisted of screening by visual assessment and completion of a lifestyle questionnaire, followed by deferral of practicing homosexual and bisexual male donors and intravenous drug addicts. The visual assessment was replaced by tests for antibodies directed against HIV, from the middle 1980‟s. In the early 1990‟s HIV was increasingly found in the black population of South Africa, particularly among black women. By 1998 0.26% of the received donations returned a positive test for HIV-1. In 1999 the South African Blood Transfusion Service (SABTS) Blood Safety Policy was introduced, including a donation HIV-risk categorization model which used the donor ethnic group, gender and donation history as indicators of the risk of exposure to HIV. The unacceptable use of the donor ethnic group as an indicator was the motivation to seek a suitable alternative donation risk categorization model which excludes the donor‟s ethnic group. The use of a more acceptable model with a high level of accuracy in predicting the risk of exposure to HIV has the potential of contributing to the reduced risk of HIV transmission through blood transfusion in South Africa. The aim of this study was to compare the suitability of four alternative models based on the information obtained from donors. Donations from new and lapsed donors were categorized in the highest applicable risk category in each model. The study was divided into two phases to achieve the aim. The first phase needed to determine suitable parameters for a model which uses the donor‟s age as an indicator. For this phase the ages of the regular donors returning an HIV-positive test result, were analysed. The second phase was to evaluate the effectiveness of the four suggested alternative blood donation risk categorization models against the model introduced by the SABTS in 1999. During this phase the donor demographic data and donation histories of donors who made donations at the Bloemfontein branch of the South African National Blood Service (SANBS) between October 2004 and September 2005, were analysed statistically. This phase honed in on two aspects to evaluate the effectiveness of the alternative models. Firstly the percentages of HIV-positive donations found in each risk category of each model, were determined as indicators of the residual risk of HIV-positive donations within the window period. Secondly the percentages of the collected blood donations allocated to each risk category within each model, were analysed to give an indication of the availability of “safe” blood associated with each of the models. The first phase of the study highlighted the difference in the age-group prevalence between male and female regular donors who returned an HIVpositive test result. Potentially suitable parameters for an Age-based Model were formulated by comparing this data with the ages of the donors who donated in Bloemfontein during the twelve months covered by this study. The second phase compared a Donation Interval Model, a Combination Model (using donation interval, gender and ethnic group as indicators), the SANBS 2005 Model (using age and gender as indicators) and an Agebased Model (using age and gender as indicators) with the SABTS 1999 Model (using gender and ethnic group as indicators). This study has shown that each of the models analysed has its advantages and disadvantages. The SANBS 2005 Model proved the best model without an ethnic indicator, for SANBS. Several recommendations regarding further investigation emanating from the results of this study were made.

Aim: The aim of this study was to explore the attitudes and perceptions of health care professionals to alternatives to blood transfusion for Jehovah's Witnesses patients in a critical care setting. Methodology: Quantitative and qualitative approaches were used to explore the attitudes and perceptions of health care professional to alternatives to blood transfusion, in a critical care setting. The study was conducted in four largest hospitals in the Durban Metropolitan area, of which two were public and two private. A questionnaire was used as tool for collecting data. Space was provided to accommodate comments from the respondents in the questionnaire. Analysis of findings was done quantitatively by using a computer programme called SPSS (Statistical Packages for the Social Sciences), version 11.5. Themes that emerged from the participant's responses were classified under categories in the conceptual framework and analysed qualitatively. Findings: Most of the respondents had mixed feelings about using alternatives to blood transfusion. The study revealed, however, that although alternatives to blood transfusions were commonly used in a critical care setting, some of the respondents did not know about the different types of alternatives available. Furthermore, it was evident from the respondent's comments that most of them had a positive attitude to people who refused blood transfusion and preferred alternatives. Research into suitable alternatives to blood transfusion was encouraged by most of the respondents. However, transfusion transmitted diseases emerged as a current concern over and above the religious concerns. Recommendations for the future were made for the health care professionals working in critical care setting, nurse educators and nursing management. / Thesis (M.N.)-University of Natal, Durban, 2004.