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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
71

Efeito de um programa de exerc?cio funcional associado ? terapia laser de baixa intensidade na dor, capacidade funcional e qualidade de vida em indiv?duos com fibromialgia: ensaio cl?nico randomizado duplo-cego

Maciel, Daniel Germano 02 March 2017 (has links)
Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-04-03T22:49:32Z No. of bitstreams: 1 DanielGermanoMaciel_DISSERT.pdf: 1593169 bytes, checksum: 5afe86923f3e38d947bd98013d678865 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-04-11T21:40:36Z (GMT) No. of bitstreams: 1 DanielGermanoMaciel_DISSERT.pdf: 1593169 bytes, checksum: 5afe86923f3e38d947bd98013d678865 (MD5) / Made available in DSpace on 2017-04-11T21:40:36Z (GMT). No. of bitstreams: 1 DanielGermanoMaciel_DISSERT.pdf: 1593169 bytes, checksum: 5afe86923f3e38d947bd98013d678865 (MD5) Previous issue date: 2017-03-02 / A fibromialgia ? uma doen?a que apresenta dor musculoesquel?tica cr?nica e difusa associada a fadiga, dist?rbio do sono, problemas cognitivos e baixos n?veis de condicionamento f?sico. Trabalhos recentes mostram bons resultados tanto com a pr?tica de exerc?cios funcionais quanto com a utiliza??o da terapia laser de baixa intensidade (TLBI) para reduzir os sintomas dessa popula??o. Por?m, n?o foram encontradas evid?ncias que associem as duas formas de terapia no tratamento da fibromialgia. Objetivo: Investigar os efeitos de um programa de exerc?cios funcionais associado ? TLBI na dor, desempenho funcional e qualidade de vida de indiv?duos com fibromialgia. M?todos: Trata-se de um ensaio cl?nico randomizado e duplo-cego composto por 22 mulheres divididas em dois grupos de forma aleat?ria em blocos: grupo placebo (n=11) que foi submetido a um programa de exerc?cios funcionais associado a TLBI placebo; e o grupo laser (n=11) que foi submetido ao mesmo programa de exerc?cios associado a TLBI ativa. O programa de exerc?cio teve dura??o de dois meses e frequ?ncia de tr?s vezes semanais. A TLBI (808nm; 100mW; 4J e 142,85 J/cm2 por ponto) foi aplicada imediatamente ap?s cada sess?o de exerc?cio em diferentes pontos dos m?sculos quadr?ceps (8), isquiotibiais (6) e tr?ceps sural (3), bilateralmente. Todos os indiv?duos foram submetidos a avalia??es pr? e p?s interven??o quanto a: dor (locais, intensidade e limiar), desempenho funcional (equil?brio, testes funcionais), desempenho muscular (flexibilidade e vari?veis isocin?ticas), depress?o e qualidade de vida. A an?lise estat?stica foi realizada por meio do SPSS 20.0 adotando um intervalo de confian?a de 95%. Resultados: Pode-se observar que houve uma redu??o da dor e melhora do desempenho funcional e muscular, da depress?o e qualidade de vida em ambos os grupos (p<0,05), por?m, sem diferen?as significativas entre eles (p>0,05). Conclus?o: O programa de exerc?cio funcional foi eficaz na melhora da dor, desempenho funcional e qualidade de vida de indiv?duos com fibromialgia. No entanto, a TLBI n?o promoveu um incremento nos efeitos positivos proporcionados pelo exerc?cio. / Background: Fibromyalgia is a disease that presents chronic and diffuse musculoskeletal pain associated with fatigue, sleep disturbance, cognitive problems and low levels of physical conditioning. Recent studies have shown good results both with the practice of functional exercises or with the use of low-level laser therapy (LLLT) to reduce the symptoms of this population. However, no evidence was found associating both forms of therapy in the treatment of fibromyalgia. Objective: To investigate the effects of a LLLT associated with an exercise program on pain, functional capacity and quality of life of individuals with fibromyalgia. Methods: It was performed a randomized, double-blind clinical trial with 22 women randomly divided into two blocked groups: placebo group (n = 11) who went through a functional exercise program associated with placebo LLLT and the laser group (n = 11), who underwent the same functional exercise program associated with active TLBI. The exercise program occurred in two months and three times a week. The LLLT (808nm; 100mW; 4J e 142,85 J/cm2 per point) was applied right after each exercise program session in different points of quadriceps (8), hamstrings (6) and sural tr?ceps (3), bilaterally. Both groups were submitted to an evaluation of pain (sites, intensity and threshold), functional capacity (balance, functional tests), muscle performance (flexibility and isokinetic variables), depression and quality of life, before and after the intervention. Statistical analysis was performed using SPSS 20.0 adopting a 95% confidence interval. Results: It was observed that there was a reduction of pain and depression levels and an improvement of functional capacity, muscular performance and quality of life in both groups (p<0.05), but with no significant differences among them (p>0.05). Conclusion: The functional exercise program was effective on pain relief, better functional capacity and quality of life of individuals with fibromyalgia. However, LLLT did not promote an increase on the positive effects caused by the exercise.
72

Estudo das propriedades cr?ticas do processo epid?mico por par com difus?o de pares

Santos, Frederico Lemos dos 27 October 2010 (has links)
Made available in DSpace on 2014-12-17T14:10:19Z (GMT). No. of bitstreams: 1 FredericoLS_DISSERT.pdf: 1177174 bytes, checksum: efe72b5694aaae13f9be30ff705ec1c9 (MD5) Previous issue date: 2010-10-27 / The pair contact process - PCP is a nonequilibrium stochastic model which, like the basic contact process - CP, exhibits a phase transition to an absorbing state. While the absorbing state CP corresponds to a unique configuration (empty lattice), the PCP process infinitely many. Numerical and theoretical studies, nevertheless, indicate that the PCP belongs to the same universality class as the CP (direct percolation class), but with anomalies in the critical spreading dynamics. An infinite number of absorbing configurations arise in the PCP because all process (creation and annihilation) require a nearest-neighbor pair of particles. The diffusive pair contact process - PCPD) was proposed by Grassberger in 1982. But the interest in the problem follows its rediscovery by the Langevin description. On the basis of numerical results and renormalization group arguments, Carlon, Henkel and Schollw?ck (2001), suggested that certain critical exponents in the PCPD had values similar to those of the party-conserving - PC class. On the other hand, Hinrichsen (2001), reported simulation results inconsistent with the PC class, and proposed that the PCPD belongs to a new universality class. The controversy regarding the universality of the PCPD remains unresolved. In the PCPD, a nearest-neighbor pair of particles is necessary for the process of creation and annihilation, but the particles to diffuse individually. In this work we study the PCPD with diffusion of pair, in which isolated particles cannot move; a nearest-neighbor pair diffuses as a unit. Using quasistationary simulation, we determined with good precision the critical point and critical exponents for three values of the diffusive probability: D=0.5 and D=0.1. For D=0.5: PC=0.89007(3), &#946;/v=0.252(9), z=1.573(1), =1.10(2), m=1.1758(24). For D=0.1: PC=0.9172(1), &#946;/v=0.252(9), z=1.579(11), =1.11(4), m=1.173(4) / O processo de contato por par -PCP ? um modelo estoc?stico de n?o equil?brio que se inspira no processo de contato simples -PC e que exibe uma transi??o de fase para um estado absorvente. Embora que o estado absorvente para o PC corresponda a uma ?nica configura??o (estado vazio), o PCP possui infinitas configura??es. No entanto, estudos num?ricos e te?ricos indicam que o PCP pertence a mesma classe de universalidade do PC (classe da percola??o direcionada), mas apresenta uma anomalia na din?mica de propaga??o. Um n?mero infinito de configura??es de estados absorventes surge no PCP, devido a todos os processos de cria??o e aniquila??o que requererem um par de part?culas de vizinhos mais pr?ximos. O processo de contato por par difusivo - PCPD foi proposto por Grassberger em 1982. Por?m, o interesse neste problema segue com a redescoberta por Howard; T?uber (1997), que questionaram a validade da descri??o de Langevin. Com base nos resultados num?ricos e em grupo de renormaliza??o, Carlon; Henkel ; Schollw?ck, (2001), observaram que alguns expoentes cr?ticos no PCPD apresentam valores similares ao da classe PC. Porem, Hinrichsen (2001), mostrou resultados diferentes do caso PCPD, atrav?s da simula??o, para o caso PC, propondo uma nova classe de universalidade. At? hoje existe uma controv?rsia em rela??o a classe de universalidade do PCPD. No PCPD ? necess?rio um par de part?culas vizinhas para os processos de cria??o e aniquila??o, embora as part?culas difundam individualmente. Neste trabalho, estudamos o PCPDP com difus?o de pares, no qual part?culas isoladas n?o podem difundir. Pares vizinhos difundem juntos. Usando simula??o quase-estacion?ria, determinamos com boa precis?o o ponto cr?tico e os expoentes para dois valores da probabilidade de difus?o: D=0.5, e 0.1. Para D=0.5: PC=0.89007(3), &#946;/v=0.252(9), z=1.573(1), =1.10(2), m=1.1758(24). Para D=0.1: PC=0.9172(1), &#946;/v=0.252(9), z=1.579(11), =1.11(4), m=1.173(4)
73

Estudo do equil?brio l?quido-vapor do sistema ?gua+etanol+l?quido i?nico visando a separa??o do ?lcool anidro

Maciel, J?ssica Caroline da Silva Linhares 21 September 2012 (has links)
Made available in DSpace on 2014-12-17T15:01:30Z (GMT). No. of bitstreams: 1 JessicaCSLM_DISSERT.pdf: 2011571 bytes, checksum: 8e087d35d6e4c83d4f7eda24cd8d3e57 (MD5) Previous issue date: 2012-09-21 / Anhydrous ethanol is used in chemical, pharmaceutical and fuel industries. However, current processes for obtaining it involve high cost, high energy demand and use of toxic and pollutant solvents. This problem occurs due to the formation of an azeotropic mixture of ethanol + water, which does not allow the complete separation by conventional methods such as simple distillation. As an alternative to currently used processes, this study proposes the use of ionic liquids as solvents in extractive distillation. These are organic salts which are liquids at low temperatures (under 373,15 K). They exhibit characteristics such as low volatility (almost zero/ low vapor ), thermal stability and low corrosiveness, which make them interesting for applications such as catalysts and as entrainers. In this work, experimental data for the vapor pressure of pure ethanol and water in the pressure range of 20 to 101 kPa were obtained as well as for vapor-liquid equilibrium (VLE) of the system ethanol + water at atmospheric pressure; and equilibrium data of ethanol + water + 2-HDEAA (2- hydroxydiethanolamine acetate) at strategic points in the diagram. The device used for these experiments was the Fischer ebulliometer, together with density measurements to determine phase compositions. The experimental data were consistent with literature data and presented thermodynamic consistency, thus the methodology was properly validated. The results were favorable, with the increase of ethanol concentration in the vapor phase, but the increase was not shown to be pronounced. The predictive model COSMO-SAC (COnductor-like Screening MOdels Segment Activity Coefficient) proposed by Lin & Sandler (2002) was studied for calculations to predict vapor-liquid equilibrium of systems ethanol + water + ionic liquids at atmospheric pressure. This is an alternative for predicting phase equilibrium, especially for substances of recent interest, such as ionic liquids. This is so because no experimental data nor any parameters of functional groups (as in the UNIFAC method) are needed / O etanol anidro tem ampla aplica??o em ind?strias qu?mica, farmac?utica e de combust?veis. No entanto, os processos atuais para a sua obten??o envolvem custo elevado, alta demanda de energia e a utiliza??o de solventes t?xicos e poluentes. Esse problema ocorre devido ? forma??o de aze?tropo na mistura etanol + ?gua, n?o permitindo a separa??o completa por m?todos convencionais tais como destila??o simples. Como uma alternativa aos processos atualmente utilizados, este estudo prop?e a utiliza??o de l?quidos i?nicos como agentes na destila??o extrativa. Trata-se de sais org?nicos l?quidos a baixas temperaturas (abaixo de 373,15 K). Suas caracter?sticas, tais como baixa volatilidade (press?o de vapor muito baixa), estabilidade t?rmica e baixa corrosividade os tornam interessantes para aplica??es como catalisadores e solventes. Neste trabalho, dados experimentais de press?o de vapor de etanol e ?gua puros na faixa de press?o de 20 a 101 kPa, assim como dados de equil?brio l?quido-vapor(ELV) do sistema etanol + ?gua a press?o atmosf?rica, e dados de equil?brio do sistema etanol + ?gua + 2-HDEAA (acetato de 2-hidrodietanolamina) foram obtidos em pontos estrat?gicos do diagrama. O dispositivo usado para esses experimentos foi o ebuliometro de Fischer, juntamente com medidas de densidade para determinar a composi??o das fases. Os dados obtidos apresentaram coer?ncia com dados da literatura e consist?ncia termodin?mica, desta forma, a metodologia foi devidamente validada. Os resultados foram favor?veis, com o aumento da concentra??o de etanol na fase vapor, por?m o aumento n?o demonstrou ser elevado. O modelo preditivo COSMO-SAC (COnductor-like Screening MOdels Segment Activity Coefficient), desenvolvido por Lin e Sandler (2002), foi aplicado para os c?lculos de predi??o de Equil?brio L?quido-Vapor do sistema etanol + ?gua com l?quido i?nico a press?o atmosf?rica. Essa ? uma alternativa para a previs?o do equil?brio de fases, especialmente para subst?ncias de interesse recente, tais como l?quidos i?nicos, uma vez que nem dados experimentais, nem par?metros de grupos funcionais (como no m?todo UNIFAC) s?o necess?rios
74

Conceitos e t?cnicas da mec?nica estat?stica e termodin?mica aplicados ao estudo dos grafos aleat?rios

Vieira, Tiago de Medeiros 24 February 2012 (has links)
Made available in DSpace on 2014-12-17T15:14:59Z (GMT). No. of bitstreams: 1 TiagoMV_DISSERT.pdf: 2054032 bytes, checksum: 01cc6c903e54670f8c4b846fab645cd0 (MD5) Previous issue date: 2012-02-24 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / This dissertation briefly presents the random graphs and the main quantities calculated from them. At the same time, basic thermodynamics quantities such as energy and temperature are associated with some of their characteristics. Approaches commonly used in Statistical Mechanics are employed and rules that describe a time evolution for the graphs are proposed in order to study their ergodicity and a possible thermal equilibrium between them / Esta disserta??o apresenta brevemente os grafos aleat?rios e as principais quantidades calculadas a partir deles. Ao mesmo tempo, grandezas b?sicas da Termodin?mica como energia e temperatura s?o associadas a algumas de suas caracter?sticas. Abordagens comumente utilizadas na Mec?nica Estat?stica s?o empregadas e regras que descrevem uma evolu??o temporal para os grafos s?o propostas com o objetivo de estudar sua ergodicidade e um poss?vel equil?brio t?rmico entre eles
75

Validade e reprodutibilidade do Wii Balance Board para avalia??o do equil?brio vertical est?tico: um novo m?todo de avalia??o.

Silva, Nathalia Priscila Oliveira 27 February 2014 (has links)
Made available in DSpace on 2014-12-17T15:16:20Z (GMT). No. of bitstreams: 1 NathaliaPOS_DISSERT.pdf: 1603301 bytes, checksum: 3c0c1f2df3c4b9a36151d032eba2a9d1 (MD5) Previous issue date: 2014-02-27 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The Wii Balance Board (WBB) began to be investigated as a low-cost alternative for assessing static balance in vertical posture. However, studies employed methodological procedures that did not eliminate result variability between the tests and equipment used. Objective: Determine the validity and reproducibility of the WBB as an instrument for assessing static balance in the vertical position, using simultaneous data analysis and superimposed equipment. Methods: This is an accuracy study of 29 healthy young individuals of both sexes aged 18 to 30 years. Subjects were assessed 24h apart (test-retest), using unipodal and bipodal support tests, with eyes closed and open. To that end the WBB was placed on top of a force platform (FP) and data (postural sway) were collected simultaneously on both devices. Validity and reproducibility were analyzed using the interclass correlation coefficient (ICC). Finally, Bland-Altman analysis was applied to assess agreement. Results: The sample was composed of 23 women and 6 men, with mean age of 24.2?6.3 years, 60.7?6.3 kg and 1.64?4.2 m. The validity of the WBB compared to the FP was excellent for all 4 tasks proposed (ICC = 0.93 0.98). The reproducibility analyzed by test-retest was excellent for the bipodal support tasks (ICC = 0.93-0.98) and only moderate for the unipodal support tests (ICC = 0.46 0.70). Graphic analysis exhibited good agreement between the devices, since most of the measures were within the limits of agreement. Conclusion: this study proved the validity and reproducibility of the Wii Balance Board as an instrument for assessing static balance in vertical posture, using simultaneous analysis with superimposed equipment. Thus, the WBB has been increasingly used by physical therapists and other health professionals in their clinical practice, as both a rehabilitation and assessment tool / O Wii Balance Board (WBB) passou a ser investigado como uma alternativa de baixo custo para avalia??o do equil?brio est?tico na postura vertical. No entanto, as pesquisas utilizaram procedimentos metodol?gicos que n?o eliminaram a variabilidade de resultados existente entre os testes e equipamentos utilizados. Objetivo: Averiguar as propriedades de validade e reprodutibilidade do WBB como instrumento para avalia??o do equil?brio est?tico na postura vertical, por meio do m?todo de an?lise simult?nea de dados com a sobreposi??o de equipamentos. M?todos: Trata-se de um de estudo de acur?cia, no qual foram avaliados 29 jovens saud?veis de ambos os sexos de 18-30 anos. Os inviv?duos foram avaliados em 2 momentos distintos com intervalo de 24h (teste-reteste), atrav?s de testes de apoio unipodal e bipodal com olhos fechados e abertos. Para isso o WBB foi colocado sobre a Plataforma de for?a (PF) e a coleta dos dados (deslocamento total do Centro de Press?o) foi feita simultaneamente em ambos equipamentos. A validade e reprodutibilidade foram analisadas atrav?s do coeficiente de correla??o intraclasse (CCI). Por fim, foi feita uma an?lise de concord?ncia por meio do m?todo de Bland-Altman. Resultados: A amostra foi composta por 23 mulheres e 6 homens, com idade m?dia de 24,2?6,3 anos, 60,7?6,3 kg e 1,64?4,2 m. A validade do WBB em compara??o com a PF mostrou-se excelente para todas as 4 tarefas propostas (CCI = 0,93 0,98). A reprodutibilidade analisada atrav?s do teste-reteste mostrouse excelente para as tarefas de apoio bipodal (CCI = 0,93 0,88) e apenas moderada para os testes de apoio unipodal (CCI = 0,46 0,70). Na an?lise gr?fica, viu-se uma boa concord?ncia entre os dispositivos, j? que a maioria das medidas encontra-se dentro dos limites concord?ncia. Conclus?o: esta pesquisa provou a validade e reprodutibilidade do Wii Balance Board como um instrumento para avalia??o do equil?brio est?tico na postura vertical, atrav?s da an?lise simult?nea com sobreposi??o de equipamentos. Tornando o WBB cada vez mais vi?vel para sua utiliza??o na pr?tica cl?nica pelos fisioterapeutas e diversos profissionais de sa?de, n?o apenas como uma ferramenta de reabilita??o mas tamb?m de avalia??o
76

An?lise do comportamento da barragem engenheiro Armando Ribeiro Gon?alves - A?u/RN - durante sua constru??o, primeiro enchimento e opera??o do reservat?rio / Analysis of the Armando Ribeiro Gon?alves dam behavior - A?u/RN - during its construction, first filling and operation of the reservoir

Souza, Andriele Nascimento de 30 March 2017 (has links)
Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-06-02T22:25:24Z No. of bitstreams: 1 AndrieleNascimentoDeSouza_DISSERT.pdf: 14510659 bytes, checksum: b589d39cc71e5725449a5974847ba202 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-06-08T21:37:57Z (GMT) No. of bitstreams: 1 AndrieleNascimentoDeSouza_DISSERT.pdf: 14510659 bytes, checksum: b589d39cc71e5725449a5974847ba202 (MD5) / Made available in DSpace on 2017-06-08T21:37:57Z (GMT). No. of bitstreams: 1 AndrieleNascimentoDeSouza_DISSERT.pdf: 14510659 bytes, checksum: b589d39cc71e5725449a5974847ba202 (MD5) Previous issue date: 2017-03-30 / A Barragem Eng. Armando Ribeiro Gon?alves, mais conhecida como Barragem de A?u, ? uma obra geot?cnica de expressiva notoriedade e import?ncia no Rio Grande do Norte e no Nordeste. Al?m do seu porte ? ? a maior barragem do RN ? e import?ncia, ganhou destaque na literatura nacional e internacional pelo rompimento do talude de montante no final do per?odo construtivo. A partir da an?lise dos dados de instrumenta??o dispon?veis no meio acad?mico, o presente trabalho teve como objetivo analisar o comportamento da obra nas etapas de constru??o do maci?o, primeiro enchimento e opera??o do reservat?rio. Em cada etapa, foi analisado: o comportamento tens?o-deforma??o; a varia??o de poro-press?es; e a estabilidade de taludes. As an?lises foram feitas com os softwares Sigma/W, Seep/W e Slope/W, pertencentes ao pacote Geo-Studio, da empresa Geo-Slope. Os resultados das modelagens foram comparados aos dados da instrumenta??o, dispon?veis em Rocha (2003). Na an?lise comparativa dos deslocamentos verticais, a maior diferen?a entre os resultados da modelagem e os dados da instrumenta??o foi de 8,9 cm, no extens?metro T7, no final do primeiro enchimento. Na an?lise da estabilidade, o fator de seguran?a mais baixo foi de 1,3, fornecido pelo m?todo dos elementos finitos, para o talude de montante, na fase de opera??o do reservat?rio. Entretanto, a m?dia dos fatores de seguran?a obtidos pelos m?todos baseados no equil?brio-limite nesta etapa foi de 2,4. Para o talude de jusante, esse fator foi de 1,6 em todas as etapas. Esses resultados comprovam a condi??o est?vel da barragem de A?u. Na an?lise de poropress?es, os piez?metros pneum?ticos apresentaram maiores diverg?ncias, entre modelagem e instrumenta??o, do que os de tubo aberto. A diferen?a mais expressiva foi de 46% (23 kPa), no piez?metro PZP1, no primeiro enchimento do reservat?rio. Entretanto, considerando as diferen?as obtidas por outros autores que tamb?m estudaram o fluxo nesta barragem, conclui-se que os resultados da an?lise s?o satisfat?rios e que os par?metros adotados s?o adequados para caracterizar os solos modelados. / The A?u Dam is an important geotechnical built in Rio Grande do Norte and in Northeast Brazilian, especially because of its size and complexity. In addition to its size and importance, it has gained prominence in the national and international literature because of the collapse in upstream slope in the end of construction period. This paper aimed to analyze its behavior in stages of dam construction, first filling and operation of the reservoir. In each step, were analyzed: the stress-strain behavior, the flow and the pore-pressure variation, and the slope stability. These analyses were performed with the software Sigma/W, Seep/W and Slope/W, which are components in a suite of geotechnical products called GeoStudio, produced by Geo-Slope International Ltd. The results were compared with the instrumentation dates available in Rocha (2003). In the comparative analysis of vertical displacements, the largest difference between modeling results and instrumentation data was 8.9 cm, in the T7 extensometer, at the end of the first filling. In the stability analysis, the lowest safety factor was 1.3 for the upstream slope in the reservoir operation phase, but the media of the safety factors obtained by the methods based on limit equilibrium was 2.4. For a downstream slope, this factor was equal to 1.6 in all stages. These results confirm the dam stable condition. In pore-pressure analysis, the pneumatic piezometers presented greater divergences between modeling and instrumentation than those of the Casagrande type. The most significant difference was 46% (23 kPa), in the PZP1 piezometer, at the first filling of the reservoir. However, considering the differences obtained by other authors who also studied the water flow in this dam, we conclude that the results of the present analysis are satisfactory and that the adopted parameters are adequate to characterize the soils modeled.
77

Genetic analysis of resistance to Fusarium head blight in wheat (Triticum spp.) using phenotypic characters and molecular markers

Malihipour, Ali 26 October 2010 (has links)
Fusarium head blight (FHB), caused mainly by Fusarium graminearum (teleomorph: Gibberella zeae), is one of the most damaging diseases of wheat. A ‘Brio’/‘TC 67’ spring wheat population was used to map quantitative trait loci (QTLs) for resistance to FHB, and to study the association of morphological and developmental characteristics with FHB resistance. Interval mapping (IM) detected a major QTL on chromosome 5AL for resistance to disease severity (type II resistance) and Fusarium-damaged kernels (FDK) under greenhouse and field conditions, respectively. Inconsistent QTL(s) was also detected on chromosome 5BS for disease severity and index using field data. The associations of plant height and number of days to anthesis were negative with disease incidence, severity, index, and deoxynivalenol (DON) accumulation data under field conditions. However, number of days to anthesis was positively correlated with disease severity (greenhouse) and FDK (field). Awnedness had a negative effect on FHB, namely the presence of awns resulted in less disease in the population. Spike threshability also affected FHB so that the hard threshable genotypes represented lower disease. Phylogenetic relationships of putative F. graminearum isolates from different sources were characterized using Tri101 gene sequencing data. Canadian and Iranian isolates clustered in F. graminearum lineage 7 (=F. graminearum sensu stricto) within the F. graminearum clade while the isolates received from CIMMYT, Mexico were placed in F. graminearum lineage 3 (=Fusarium boothii) within the Fg clade or Fusarium cerealis. The PCR assay based on the Tri12 gene revealed the presence of the NIV, 3-ADON, and 15-ADON chemotypes with 15-ADON being the predominant chemotype. While we did not find the NIV chemotype among the Canadian isolates, it was the predominant chemotype among the Iranian isolates. High variation in aggressiveness was observed among and within Fusarium species tested, with the isolates of F. graminearum sensu stricto being the most aggressive and the NIV chemotype being the least aggressive. The interactions between Fusarium isolates and wheat genotypes from different sources were investigated by inoculating isolates of F. graminearum sensu stricto and F. boothii on wheat genotypes. Significant differences were observed among the genotypes inoculated by single isolates. Results also showed significant interactions between Fusarium isolates and wheat genotypes. The F. boothii isolates from CIMMYT produced low disease symptom and infection on wheat genotypes regardless of the origin of the genotypes while F. graminearum sensu stricto isolates from Canada and Iran resulted in higher FHB scores.
78

Genetic analysis of resistance to Fusarium head blight in wheat (Triticum spp.) using phenotypic characters and molecular markers

Malihipour, Ali 26 October 2010 (has links)
Fusarium head blight (FHB), caused mainly by Fusarium graminearum (teleomorph: Gibberella zeae), is one of the most damaging diseases of wheat. A ‘Brio’/‘TC 67’ spring wheat population was used to map quantitative trait loci (QTLs) for resistance to FHB, and to study the association of morphological and developmental characteristics with FHB resistance. Interval mapping (IM) detected a major QTL on chromosome 5AL for resistance to disease severity (type II resistance) and Fusarium-damaged kernels (FDK) under greenhouse and field conditions, respectively. Inconsistent QTL(s) was also detected on chromosome 5BS for disease severity and index using field data. The associations of plant height and number of days to anthesis were negative with disease incidence, severity, index, and deoxynivalenol (DON) accumulation data under field conditions. However, number of days to anthesis was positively correlated with disease severity (greenhouse) and FDK (field). Awnedness had a negative effect on FHB, namely the presence of awns resulted in less disease in the population. Spike threshability also affected FHB so that the hard threshable genotypes represented lower disease. Phylogenetic relationships of putative F. graminearum isolates from different sources were characterized using Tri101 gene sequencing data. Canadian and Iranian isolates clustered in F. graminearum lineage 7 (=F. graminearum sensu stricto) within the F. graminearum clade while the isolates received from CIMMYT, Mexico were placed in F. graminearum lineage 3 (=Fusarium boothii) within the Fg clade or Fusarium cerealis. The PCR assay based on the Tri12 gene revealed the presence of the NIV, 3-ADON, and 15-ADON chemotypes with 15-ADON being the predominant chemotype. While we did not find the NIV chemotype among the Canadian isolates, it was the predominant chemotype among the Iranian isolates. High variation in aggressiveness was observed among and within Fusarium species tested, with the isolates of F. graminearum sensu stricto being the most aggressive and the NIV chemotype being the least aggressive. The interactions between Fusarium isolates and wheat genotypes from different sources were investigated by inoculating isolates of F. graminearum sensu stricto and F. boothii on wheat genotypes. Significant differences were observed among the genotypes inoculated by single isolates. Results also showed significant interactions between Fusarium isolates and wheat genotypes. The F. boothii isolates from CIMMYT produced low disease symptom and infection on wheat genotypes regardless of the origin of the genotypes while F. graminearum sensu stricto isolates from Canada and Iran resulted in higher FHB scores.

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