Šunų babeziozės sukėlėjo Babesia canis 18S rRNR geno sekų palyginamoji analizė / Sequence analysis of 18S rRNR gene of canine babesiosis causative agent Babesia canisFrolovienė, Jurga 11 June 2014 (has links)
Šunų babeziozė – tai liga, greitai plintanti visame pasaulyje, kurią sukelia pirmuonys, priklausantys Babesia genčiai. Ligos sukėlėjus platina Dermocentor, Rhipicephalus, Haemaphysalis genties erkės. Lietuvoje paplitę Dermacentor reticulatus erkės - pagrindiniai Babesia canis vektoriai. B. canis patenka į raudonąsias kraujo ląsteles (eritrocitus) ir jas suardo. Kasmet sergamumas šunų babezioze visoje Europoje bei Lietuvoje stipriai auga. Darbo tikslas buvo įvertinti šunų užsikrėtimą Babesia canis patogenais ir identifikuoti Babesia canis porūšius ir padermes, panaudojant molekulinius tyrimo metodus ir filogenetinę sekų analizę. Šiame darbe buvo tiriami 20 šunų, sergančių babezioze,( pagal babeziozei būdingą klinikinę išraišką) kraujo mėginiai, kurie buvo surinkti iš Kauno apskrities Veterinarijos klinikų. Taikant molekulinį PGR (polimerazės grandininės reakcijos) metodą, 17 iš šių mėginių buvo identifikuota Babesia canis canis. Atlikus palyginamąją Babesia canis canis 18S rRNR geno sekų analizę, pasitelkus Gen Bank duomenis, buvo nustatyta, kad Lietuvoje sekvenuotos sekos nuo užsikrėtusių šunų buvo identiškos tarpusavyje ir turėjo 91-100% panašumą su sekomis, identifikuotomis sergančiuose šunyse ir D. reticulatus erkėse kitose Europos šalyse. Identifikuotos sekos priklausė vienam iš genotipų, paplitusių Europoje, kuris pasižymi didesniu virulentiškumu. Europos šalyse nustatyta 10 B. canis canis sekų variantų, identifikuotų pagal 18S rRNR geno sekų analizę. / Canine babesiosis is a disease, quickly spreading worldwide, which is caused by the protozoa belonging to the genus Babesia. The disease agents are transmitted by ticks of the genera Dermocentor, Rhipicephalus, and Haemaphysalis. Dermacentor reticulatus ticks, which are widely spread in Lithuania, are the main vectors of Babesia canis. B. canis infects red blood cells (erythrocytes) and destroys them. Every year the morbidity of Babesia canis is intensely growing in the whole of Europe and Lithuania. The aim of thesis was to evaluate the canine infection with Babesia canis pathogens and identify species and subspecies of Babesia canis, using molecular methods of analysis and phylogenetic sequence. The research focused on 20 dogs infected with Babesia canis (in accordance with the clinical expression characteristic of babesiosis); the blood samples were collected in the veterinary clinics in the Kaunas region. Applying the molecular PCR (Polymerase chain reaction) method, 17 of the samples were identified as cases of Babesia canis canis. On the basis of the contrastive gene sequencing analysis of Babesia canis canis 18S rRNR and the data of Gen Bank it was determined that in Lithuania the sequential sequences of infected dogs were identical and by 91-100 percent resembled the sequences identified in infected dogs and D. reticulatus ticks in other European countries. The identified sequences belonged to one of the genotypes which is widely spread in Europe and is... [to full text]
The scintigraphic evaluation of the pulmonary perfusion pattern of dogs hospitalised with babesiosisSweers, Lynelle 08 May 2008 (has links)
A hypercoagulable state has been demonstrated in human falciparum malaria in mild and complicated forms of the disease. Disseminated intravascular coagulation (DIC) was implicated by some authors, but deemed a rare occurrence by others. The possibility of coagulopathy in Babesia canis rossi infections in the canine patient has also been suggested in the literature, but minimal work has been done to evaluate the clinicopathological nature of it in further detail. In the canine babesiosis (CB) pathogenesis thought-process, DIC has been implicated. A DIC-like syndrome, as evidenced by intravascular fibrin deposition and haemorrhage into muscles and tissues was found at post mortem in one study. On the basis of these findings, it was postulated that DIC might be a serious complication of severe Babesia infection in the dog. Clinical DIC (haemorrhagic diathesis) is however seldom seen. It was also hypothesised in the literature that the multiple organ dysfunction syndrome (MODS) demonstrated in the complicated form of Babesia was caused, in addition to tissue damage due to local hypoxia, by microthrombi as a result of a coagulopathy. This needs to be further investigated. Pulmonary thromboembolism (PTE) has not been implicated in CB, however thromboemboli in the lungs were found in dogs with immune-mediated haemolytic anaemia (IMHA) for which a similar mechanism of venous stasis, hypercoagulability and endothelial damage (as found in CB) is proposed. In humans, PTE is believed to be a major underdiagnosed contributor to mortality in 5 to 15% of hospitalised adults. If early diagnosis of PTE can be achieved, the mortality rate can certainly be decreased. A similar situation with resultant serious implications in complicated CB cases may exist. Clinically, PTE is suspected if a patient with a known prothrombotic condition develops sudden dyspnoea and tachypnoea. These clinical symptoms are frequently seen in complicated CB patients and may, in addition to being a compensatory mechanism for the metabolic acidosis and anaemia, be attributed to thrombus-induced mechanical changes in lung function. Pulmonary scintigraphy provides a sensitive means of diagnosing PTE. It was (and some authors still do) believed that a ventilation scintigraphic scan should be done in association with a perfusion scan to increase the specificity and accuracy of diagnoses. However, authors of the recent PISA-PED study in humans proposed that the sensitivity and specificity of a perfusion scan, without a ventilation scan, in patients with suspected PTE was sufficient. The incidence of PTE or the use of pulmonary perfusion scintigraphy in CB dogs has never been studied. The objective of this study was to prospectively evaluate the scintigraphic pulmonary perfusion pattern in hospitalised Babesia dogs in an attempt to ascertain whether a scintigraphic pattern consistent with PTE does indeed occur in these patients. The study consisted of a normal control group of nine mature healthy Beagle dogs aged 36 – 43 months and weighing 9.9 – 15kg and a Babesia group with 14 dogs of a variety of breeds that were naturally infected with Babesia, aged 6 – 103 months and weighing 6.3 – 25.5kg. Pulmonary perfusion scintigraphy was performed after making thoracic radiographs and performing a blood gas analysis in both groups. The scintigraphic images were visually inspected for changes suggestive of PTE. Surprisingly, not a single dog in the Babesia group had segmental or wedge-shaped perfusion defects which would have resulted in a high probability for PTE. The scintigraphic pulmonary perfusion pattern demonstrated was not significantly different between the two groups (p = 1.00). Many dogs in both groups had a mottled appearance on the right and left dorsal oblique images, which was not believed to be consistent with clinically relevant PTE. This study provides baseline data that may be used to further investigate the pulmonary perfusion pattern in Babesia dogs. / Dissertation (MMedVet (Diagnostic Imaging))--University of Pretoria, 2007. / Companion Animal Clinical Studies / unrestricted
Serum concentrations of tumour necrosis factor in dogs naturally infected with Babesia Canis and its relation to severity of diseaseVaughan-Scott, Tarquin 07 November 2005 (has links)
Please read the abstract in the section 00front of this document / Canine babesiosis, caused by the tick-borne protozoan Babesia canis rossi, is an economically important and potentially fatal disease of dogs in South Africa. The host's response to many infectious diseases is mediated (at least in part) by intercellular messengers called cytokines. One of the most important cytokines released is tumour necrosis factor (TNF). A study was designed to measure serum concentrations of TNF in dogs naturally infected with canine babesiosis and to relate TNF concentrations to clinical severity, mortality, rectal temperature and parasitaemia. There was a statistically significant difference in TNF concentrations between groups of differing disease severity, with a general trend of increasing mean 10g(TNF) with increasing severity of disease. A noteworthy finding was that dogs with hypoglycaemia had very high TNF (mean 15.03 nglml compared to a mean of 2.32 nglml for other sick dogs without hypoglycaemia). When TNF values were compared between survival and non-survival groups, there was no significant difference. The rectal temperature of the dogs in this study did not show any statistically significant association with TNF concentrations. When parasitaemia and TNF were examined within groups of infected dogs, there was no significant relationship. However, when the sample size was increased by pooling all infected dogs and treating them as a single group, there was a highly significant positive correlation (p = 0.003) between parasitaemia and serum TNF concentrations. The results ofthis study were encouraging and indicate that canine babesiosis may share a similar pathophysiology with human malaria in terms ofTNF being associated with disease severity. One ofthe most significant findings in this study was the presence ofvery high TNF values in two ofthree dogs with hypoglycaemia. Hypoglycaemia has not been previously recorded in dogs with babesiosis and is a potentially important finding particularly in view ofthe hypoglycaemia associated with malaria in humans. Malarial hypoglycaemia is correlated with a higher mortality in humans, especially in pregnant women and children. If the findings ofthis study can be Vl confinned and expanded, they may lend further support to the use of canine babesiosis as a model for some ofthe problems encountered in human malaria research. / Dissertation (MMed Vet (Med))--University of Pretoria, 2001. / Companion Animal Clinical Studies / unrestricted
Analyse du rôle des antigènes parasitaires solubles de Babesia canis dans la pathogénèse de la piroplasmose canine et caractérisation moléculaire de l’antigène Bc28.2 codé par la famille multigénique Bc28. / Analysis of the function of Babesia canis soluble parasite antigens during the pathogenesis of canine piroplasmosis and molecular characterization of the Bc28.2 antigen encoded by the Bc28 multigene family.Finizio, Anne-Laure 14 December 2010 (has links)
Babesia canis est un hémoparasite du phylum des Apicomplexes transmis par la morsure de tique et responsable de la piroplasmose canine en Europe. Dans la perspective de développer un vaccin recombinant, nous avons réalisé deux études visant à mieux comprendre les interactions hôte/parasite au cours du cycle érythrocytaire. Nous avons étudié d'une part le rôle des antigènes parasitaires solubles (APS) dans le déclenchement des signes cliniques et d'autre part celui de l'antigène Bc28.2 codé par la famille multigénique Bc28 dans les mécanismes d'échappement à l'hôte.Les APS de B. canis induisent une réponse protectrice anti-maladie chez les chiens vaccinés. Toutefois, leur rôle exact dans la pathogénèse reste à définir. Contrairement à ce qui est décrit dans la pathogenèse à B. bovis, nos analyses réfutent l'hypothèse qu'ils pourraient agir sur le système kallicréine-kinine plasmatique. Par contre elles suggèrent, pour la première fois chez le genre Babesia, un rôle direct des APS dans le déclenchement précoce de la réponse inflammatoire observée au cours de la pathogenèse.De part leur fonction essentielle dans la survie parasitaire, les antigènes localisés à la surface de l'érythrocyte (rôle dans l'agglutination des hématies parasitées) ou des mérozoïtes (rôle dans l'invasion) sont de bons candidats vaccins. Cependant et probablement dans une perspective d'échappement à l'hôte, ils sont codés par des familles multigéniques. Chez B. canis, la famille multigénique Bc28 contient le gène Bc28.1 qui code pour un antigène à ancrage GPI préalablement caractérisé à la surface du mérozoïte. Nous montrons qu'un autre gène, désigné Bc28.2 (plusieurs copies dans le génome) contient 2 cadres de lecture chevauchant et serait capable d'exprimer des antigènes polymorphes de 28 kDa et 50 kDa par un mécanisme de recodage traductionnel +1. De façon originale, ces protéines seraient localisées non pas à la surface des mérozoïtes mais à la surface des hématies parasitées. / Babesia canis is an apicomplexan haemoparasite transmitted by tick bite and responsible of canine babesiosis in Europe. Understanding host/parasite relationships during the erythrocytic cycle is crucial for further development of a recombinant vaccine. In that way, the role of soluble parasite antigens (SPA) in the onset of clinical signs and the role of Bc28.2 antigen (encoded by the B. canis Bc28 multigene family) in host evading process were investigated.B. canis-derived SPA induce a protective anti-disease immunity in vaccinated dogs but their precise role during the pathogenesis remains unknown. In contrast to B. bovis, our analysis disproved the hypothesis that B. canis SPA could act on the plasma kallikrein-kinin system. However they strongly suggest, for the first time in the genus Babesia, a direct role of these SPA in the onset of the inflammatory response which is early observed during pathogenesis. Because of their essential function in the parasite survival, antigens located on the surface of infected erythrocyte (role in agglutination of erythrocytes) or on the surface of merozoites (role in the invasion) are good vaccine candidates.However, and probably for host evading, they are encoded by multigene families. In B. canis, the Bc28 multigene family contains the Bc28.1 gene that encodes for a GPI-anchored antigen previously characterized on the merozoite surface. We demonstrated here, that another gene designated Bc28.2, is multicopy and composed of two overlapping open reading frames (Orf1 and Orf2). It allows, though +1 programmed ribosomal frameshift, expression of polymorphic antigens of 28 kDa and 50 kDa. Unexpectedly, these proteins seem localized on the surface of parasitized erythrocytes, suggesting they play a crucial function in evading host through agglutination process of infected erythrocytes.
Van Heerden, Garth William
This study attempted to characterise 5 field stocks of B. canis, collected from various geographic areas of southern Africa in terms of pathogenicity, immune response, cross resistance and antigenic composition. This was done by means of artificial infection of two to four year old Beagle dogs. Serological tests included indirect fluorescent antibody tests, polyacrylamide gel electrophoresis , Western blotting of B. canis antigen and immune serum transfers. The ZIM1, GHT1 , QTN1 and DBN1 stocks were found to be virulent and the JHB1 stock, which might be a cardiac form of babesiosis, was found to be highly virulent. Immune responses of dogs to primary challenges were poor. A second challenge or relapse was important in the development of a resistant immunity. Heterologous challenges showed that dogs which were immune to the ZIM1, QTN1 and DBN1 stocks were partially immune to the JHB1 stock. The dog that was immune to the GHT1 stock was the only one that was totally resistant to the JHB1 stock. Western blots revealed interstock antigenic variation between the JHB1 and the other four stocks. These differences were, however, not great enough to separate the stocks taxonomically.
Detecção molecular e sorológica de Ehrlichia canis e Babesia canis em felídeos selvagens brasileiros mantidos em cativeiroAndré, Marcos Rogério [UNESP] 25 February 2008 (has links) (PDF)
Made available in DSpace on 2014-06-11T19:27:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-02-25Bitstream added on 2014-06-13T18:56:53Z : No. of bitstreams: 1 andre_mr_me_jabo.pdf: 427316 bytes, checksum: 366b68e3f9cae8ab0938b9ab1032ffb9 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Poucos relatos têm sido feitos sobre o diagnóstico da erliquiose e babesiose em felinos domésticos e selvagens brasileiros, os quais são baseados diretamente pela presença de mórulas em leucócitos e piroplasmas em eritrócitos, e indiretamente pela detecção de anticorpos anti-Ehrlichia canis. O presente estudo teve como objetivo realizar a detecção molecular de E. canis e B. canis e a presença de anticorpos da classe IgG contra esses hemoparasitas em amostras de sangue e soro, respectivamente. Neste utilizamos 72 felídeos selvagens brasileiros mantidos em cativeiro em algumas instituições e zoológicos. Pela Reação de Imunofluorescência Indireta (RIFI), dezoito (25%) e cinqüenta e três (73,6%) dos 72 animais amostrados foram sororeagentes frente aos antígenos de E. canis e B. canis, respectivamente. Na PCR para E. canis, onze (15,3%) dos 72 animais amostrados foram positivos. Os amplicons foram confirmados por seqüenciamento e o DNA de E. canis encontrado mostrou grande similaridade genética com amostras de E. canis isoladas no Brasil, México, Portugal, Grécia e Taiwan, com 98% de similaridade. Nenhuma das amostras foi positiva na PCR para B. canis. Destaca-se a importância e a primeira detecção molecular de E. canis e presença de anticorpos anti-E. canis e anti-B. canis em felídeos selvagens brasileiros mantidos em cativeiro. / Few are the reports that have been carried out on ehrlichiosis and babesiosis diagnostic in Brazilian domestic and wild felids, which are based directly on the presence of morulae in leucocytes and piroplasms in erythrocytes, and indirectly by detection of antibodies against E. canis. The aim of this study was to detect molecularly E. canis and B. canis and the presence of anti-E. canis and B. canis IgG antibodies in the blood and sera samples, respectively, from 72 Brazilian wild captive felids maintained in some instituitions and zoos. Eighteen (25.0%) and fifty-three (73.6%) out of 72 animals were seroreagent for E. canis and B. canis antigen, respectively, by IFA (Indirect Immunofluorescent Assay). Eleven (15.3%) of the 72 samples were positive for nPCR E. canis. The amplicons were confirmed by sequencing and the E. canis DNA found appeared to be closely related to E. canis samples from Brazil, Mexico, Portugal, Greece and Taiwan with 98% percent identity. None of the 72 samples were positive for B. canis by PCR. This is the first molecular detection of E. canis and presence of seroreactivity for both B. canis and E. canis in Brazilian wild captive felids.
Aspectos epidemiol?gicos da Babesia canis vogeli em c?es da Baixada Fluminense, RJ / Aspects of Babesia canis vogeli in dogs from Baixada Fluminense, RJVILELA, Joice Aparecida Rezende 30 March 2012 (has links)
Submitted by Jorge Silva (firstname.lastname@example.org) on 2017-05-05T17:41:38Z No. of bitstreams: 1 2012 - Joice Aparecida Rezende Vilela.pdf: 2330778 bytes, checksum: 444dfb2be034c128fc34d8e5ad64d910 (MD5) / Made available in DSpace on 2017-05-05T17:41:38Z (GMT). No. of bitstreams: 1 2012 - Joice Aparecida Rezende Vilela.pdf: 2330778 bytes, checksum: 444dfb2be034c128fc34d8e5ad64d910 (MD5) Previous issue date: 2012-03-30 / CAPES / The canine babesiosis, is a disease widely distributed in Brazil and worldwide, caused by? protozoa of the genus Babesia?spp. that parasitize erythrocytes and it?s transmitted by ticks? Ixodidae. This study aimed to characterize the epidemiology of canine babesiosis through? questionnaires and serological diagnosis by ELISA, diagnosis of Babesia? spp. infection in? dogs by real?time PCR technique, identify the subspecies of B. canis?by RFLP, characterize? the hematological aspects, diagnosis by direct microscopy, identification of ectoparasites and? diagnosis by real?time PCR, the agent of canine babesiosis in ticks from dogs from rural and? urban areas from Serop?dica city, RJ State. The present study evaluated 311 dogs, 167? (n=53,7%) of rural and 144 (n=46,3%) in urban areas. By ELISA technique, was found 39? dogs (12,54%) serologically positive for Babesia canis, with frequency of seropositivity of? 21,56% ???(n=36/167) in dogs of rural area and 2,08% (n=3/144) in dogs from urban area, and? therefore the rural area, the highest seropositivity (p<0,0001). The variables associated with? seropositivity were: area of residence of the dog, breed, age, presence of ticks, environment of? dog access, contact with other animals, the animal's habits, condition of cleanliness of the? environment, absence of veterinary care and animal wandering. The profile of the owner and? its management were striking features in parasitism by ticks and consequently the frequency? of seropositivity to the agent. The diagnostic by PCR real??time of blood samples from dogs? showed a frequency of 11,90% (n=37/311). The subspecies identified affecting dogs of this? region was B. canis vogeli. Of the total of 311 dogs evaluated, infestations by ectoparasites? were found in 252 (81,03%) dogs at the time of collection. Of these, 70,64% (n=178/252)? were infested with ticks. In relation to the infestation, Rhipicephalus sanguineus?was found in? 68,54% (n=122/178) of the dogs, Amblyomma cajennense?in 11,80% (n=21/178), A. ovale?in? 3,37% (n=6/178) and A. dubitatum? in 0,56% (n=1/178). Nymphs of R. sanguineus? and? Amblyomma sp. were observed in 13,48% (n=24/178) and 5,62% (n=10/178) of dogs infected? with ticks, respectively. The tick species most common in urban and rural areas was R. sanguineus, which was associated with positivity of B. canis?in dogs by molecular method.? The frequency of ticks positive for B. canis?was 5% (n=12/240). The DNA from B. canis?was? detected in nine R. sanguineus?ticks, an A. cajennense?adult and two nymphs of Amblyomma? sp. The main hematological changes in infected dogs were: anemia, thrombocytopenia and? hemoglobinemia. The study concluded that canine babesiosis caused by B. canis vogeli?occurs? in rural and urban areas from the Serop?dica being of great importance its occurrence in the? cities of the Rio de Janeiro State, which have characteristics similar expansion of urbanization? to rural areas with little distinction between the two areas and points to the importance of? diagnosis and identification of the pathogen and vectors in dogs, in order to know the? epidemiological conditions for the adoption of appropriate preventive measures, because in? addition to affect the animal health, many of these vectors are capable to transmit pathogens? to humans.? / A babesiose canina, patologia de ampla distribui??o no Brasil e no mundo, ? causada por? protozo?rios do g?nero Babesia? spp., que parasitam hem?cias e s?o transmitidos por? carrapatos Ixodidae. O presente trabalho objetivou determinar a epidemiologia da babesiose? canina atrav?s de aplica??o de question?rios e diagn?stico por ELISA, diagn?stico por? microscopia direta, diagn?stico de Babesia canis? por meio da PCR em tempo real,? identifica??o das subesp?cies de B. canis? por meio da RFLP, caracteriza??o das principais? altera??es hematol?gicas, identifica??o dos ectoparasitos e diagn?stico atrav?s da PCR em? tempo real do agente da babesiose canina em carrapatos de c?es do munic?pio de Serop?dica,? Baixada Fluminense ??RJ. No presente estudo foram avaliados 311 c?es, sendo 167 (n=53,7%)? de ?rea rural e 144 (n=46,3%) de ?rea urbana. Atrav?s do teste de ELISA, encontrou?se um? total de 39 c?es (12,54%) sorologicamente positivos para Babesia canis, com frequ?ncia de? 21,56% (n=36/167) nos c?es de ?rea rural e 2,08% (n=3/144) nos c?es de ?rea urbana, sendo,? portanto a ?rea rural, a de maior soropositividade (p<0,0001). As vari?veis que apresentaram? associa??o com a soropositividade foram: ?rea de domic?lio do c?o, defini??o racial, idade,? infesta??o por carrapatos, locais de acesso do c?o, contato com outros animais, h?bito do? animal, ambiente do c?o, aus?ncia de limpeza do ambiente, aus?ncia de assist?ncia? veterin?ria, aus?ncia de abrigo e origem errante. O perfil do propriet?rio e o manejo do c?o? apresentaram caracter?sticas marcantes no parasitismo por carrapatos e consequentemente na? frequ?ncia de soropositividade para o agente. O diagn?stico pela PCR em tempo real de? amostras sangu?neas de c?es revelou uma frequ?ncia de 11,90% (n=37/311). A subesp?cie? identificada acometendo c?es desta regi?o foi B. canis vogeli. Do total de 311 c?es avaliados,? foram observadas infesta??es por ectoparasitos em 252 (81,03%) c?es. Destes, 70,64%? (n=178/252) apresentaram?se infestados por carrapatos. Em rela??o ? infesta??o por? carrapatos, Rhipicephalus sanguineus foi encontrado em 68,54% (n=122/178) dos c?es,? Amblyomma cajennense em 11,80% (n=21/178), A. ovale em 3,37% (n=6/178) e A. dubitatum em 0,56% (n=1/178) dos c?es infestados por carrapatos. Ninfas de R. sanguineus e? Amblyomma sp. foram observadas em 13,48% (n=24/178) e 5,62% (n=10/178) dos c?es? infestados por carrapatos, respectivamente. A esp?cie de carrapato mais frequente nos meios? urbano e rural foi R. sanguineus, que apresentou associa??o com a positividade de B. canis? vogeli?nos c?es pelo m?todo molecular. A frequ?ncia de carrapatos positivos para B. canis foi? de 5% (n=12/240). O DNA de B. canis foi detectado em nove carrapatos adultos de R. sanguineus, em um adulto de A. cajennense? e em duas ninfas de Amblyomma? sp.. As? principais altera??es hematol?gicas nos c?es infectados foram: anemia, hemoglobinemia e? trombocitopenia. O estudo permitiu concluir que a babesiose canina causada por B. canis vogeli?ocorre nas ?reas rurais e urbanas do munic?pio estudado sendo de grande import?ncia a? sua ocorr?ncia nos munic?pios da Baixada Fluminense do Estado do Rio de Janeiro, os quais? apresentam caracter?sticas semelhantes de expans?o da urbaniza??o para o meio rural com? pequena distin??o entre as duas ?reas e aponta para a import?ncia do diagn?stico do pat?geno? e identifica??o de vetores nos c?es, visando o conhecimento das condi??es epidemiol?gicas? para a ado??o de medidas profil?ticas adequadas, pois al?m de afetarem a sanidade animal? muitos destes vetores s?o capazes de veicularem pat?genos transmiss?veis ao homem.??
Detecção molecular e sorológica de Ehrlichia canis e Babesia canis em felídeos selvagens brasileiros mantidos em cativeiro /André, Marcos Rogério. January 2008 (has links)
Orientadora: Rosangela Zacarias Machado / Banca: Jose Mauricio Barbante Duarte / Banca: Natalino Hajime Yoshinari / Resumo: Poucos relatos têm sido feitos sobre o diagnóstico da erliquiose e babesiose em felinos domésticos e selvagens brasileiros, os quais são baseados diretamente pela presença de mórulas em leucócitos e piroplasmas em eritrócitos, e indiretamente pela detecção de anticorpos anti-Ehrlichia canis. O presente estudo teve como objetivo realizar a detecção molecular de E. canis e B. canis e a presença de anticorpos da classe IgG contra esses hemoparasitas em amostras de sangue e soro, respectivamente. Neste utilizamos 72 felídeos selvagens brasileiros mantidos em cativeiro em algumas instituições e zoológicos. Pela Reação de Imunofluorescência Indireta (RIFI), dezoito (25%) e cinqüenta e três (73,6%) dos 72 animais amostrados foram sororeagentes frente aos antígenos de E. canis e B. canis, respectivamente. Na PCR para E. canis, onze (15,3%) dos 72 animais amostrados foram positivos. Os amplicons foram confirmados por seqüenciamento e o DNA de E. canis encontrado mostrou grande similaridade genética com amostras de E. canis isoladas no Brasil, México, Portugal, Grécia e Taiwan, com 98% de similaridade. Nenhuma das amostras foi positiva na PCR para B. canis. Destaca-se a importância e a primeira detecção molecular de E. canis e presença de anticorpos anti-E. canis e anti-B. canis em felídeos selvagens brasileiros mantidos em cativeiro. / Abstract: Few are the reports that have been carried out on ehrlichiosis and babesiosis diagnostic in Brazilian domestic and wild felids, which are based directly on the presence of morulae in leucocytes and piroplasms in erythrocytes, and indirectly by detection of antibodies against E. canis. The aim of this study was to detect molecularly E. canis and B. canis and the presence of anti-E. canis and B. canis IgG antibodies in the blood and sera samples, respectively, from 72 Brazilian wild captive felids maintained in some instituitions and zoos. Eighteen (25.0%) and fifty-three (73.6%) out of 72 animals were seroreagent for E. canis and B. canis antigen, respectively, by IFA (Indirect Immunofluorescent Assay). Eleven (15.3%) of the 72 samples were positive for nPCR E. canis. The amplicons were confirmed by sequencing and the E. canis DNA found appeared to be closely related to E. canis samples from Brazil, Mexico, Portugal, Greece and Taiwan with 98% percent identity. None of the 72 samples were positive for B. canis by PCR. This is the first molecular detection of E. canis and presence of seroreactivity for both B. canis and E. canis in Brazilian wild captive felids. / Mestre
Page generated in 0.0415 seconds