BACKGROUND: Low health literacy and numeracy are significant problems facing the United States. Recent research focuses heavily on the role health literacy and numeracy play in perception of disease risk, health care costs, all-cause mortality, and access to care; however, there has been relativity little emphasis on the relationships between health literacy or numeracy with health promotion behaviors, such as nutrition or physical activity. As our nation continues to face challenges with the high prevalence of obesity and other chronic diseases, it is increasingly important to understand the role that health literacy and numeracy play in nutrition and physical activity behaviors, as well as in the prevalence and control of chronic disease. PRIMARY AIMS: The proposed research is embedded within a larger randomized-control trial, Talking Health, which is a 2-arm behavioral trial targeting residents in eight counties in southwest Virginia with sugar-sweetened beverage (SSB) consumption as the primary outcome. The primary aims of this cross-sectional study, using baseline Talking Health data, are to 1) examine correlations among health literacy and numeracy measures, namely the Newest Vital Sign (NVS), separated by reading (NVS Reading) and math (NVS Math) scores, the Rapid Estimate of Adult Literacy in Medicine (REALM), and the Subjective Numeracy Scale (SNS); 2) explore the relationships between demographic factors and the NVS, REALM, and SNS scores; 3) determine the relationships between the NVS, REALM, and SNS and dietary quality [i.e. Health Eating Index (HEI) scores], physical activity behaviors, and anthropometric and biological variables (body mass index, blood pressure, fasting blood lipids, and fasting blood glucose); and 4) determine if NVS, REALM, and SNS scores predict metabolic syndrome (MetS), while controlling for relevant demographic factors. METHODS: Eligibility requirements for the study include being 18 years of age or older, having reliable access to a telephone, drinking ≥200 kilocalories of SSB per day, and being a resident of Southwest Virginia. Using previously validated instruments and standardized data collection protocol, a variety of baseline variables was collected on 264 participants. Health literacy was measured using the NVS and REALM and health numeracy was measured using the SNS. Dietary intake was measured via three 24-hour dietary recalls and HEI scores were calculated. Physical activity behaviors were assessed using the Godin Leisure Time Exercise Questionnaire. Weight was measured using a calibrated digital Tanita scale (Model: 310GS), height was measured using a portable research-grade stadiometer, blood pressure measurements were made with an OMRON automated oscillometric device (Model: HEM-907XL), and fasting blood samples were obtained via a finger stick and the CardioChek PA system was used to assess blood glucose, cholesterol, and triglycerides. MetS scores were determined based on an adaptation of the National Cholesterol Education Program guidelines. Statistical analysis included descriptive statistics, simple correlations (Pearson bivariate), one-way ANOVAs, and regression models. RESULTS: Of 264 enrolled participants (mean age 41.1 + 13.5 years; 92.0% Caucasian; 81.8% female; 30.6% > high school education; 42% > $15,000 annual income), 33.7% were classified as having a high probability of low health literacy or possibility of low health literacy as measured by the NVS, 19.7% had less than a high school reading level as measured by the REALM, and 45.4% had low health numeracy as measured by the SNS. Additionally, 78.8% were overweight or obese and 29.0% meet the criteria for metabolic syndrome. Nine of the ten correlations between the NVS Total, NVS Reading, NVS Math, REALM, and SNS were statistically significant (p < .01, two-tailed). NVS scores were found to be significantly different by age (F = 2.36, p = .05), race (F = 4.49, p = .03), education level (F = 20.97, p < .001), and income (F = 13.88, p < .001); while REALM scores were only significantly different by race (F = 3.74, p = .05), education level (F = 21.06, p < .001), and income (F = 6.80, p < .001). SNS scores were significantly different by gender (F = 12.40, p = .001), education level (F = 11.01, p < .001), and income (F = 14.45, p < .001). Only systolic blood pressure, diastolic blood pressure, and strength training activity was found to be significantly different by health literacy and/or numeracy level; however, when controlling for hypertension medication use and/or demographic variables, only the relationship between health literacy (i.e, NVS) and strength training activity remained significant (R2 = 0.09, p = .01). Finally, health literacy and numeracy were not found to be predictive of metabolic syndrome while controlling for demographic variables. DISCUSSION: Although numerous demographic factors were related to baseline health literacy and numeracy levels, anthropometric/biological variables, physical activity behaviors, and diet quality did not differ by health literacy and health numeracy level, with the exception of systolic blood pressure and strength training activity. This research helps to fill the gaps in the literature surrounding the prevalence of health literacy, health numeracy, and health promoting behaviors and chronic disease among rural residents in medically underserved counties in southwest Virginia. While few cross-sectional relationships were found, future research from this RCT should examine if health literacy and health numeracy moderates or mediates intervention changes in anthropometric/biological variables, physical activity behaviors, diet quality, and metabolic syndrome scores. / Master of Science

BACKGROUND: Health-related interventions informed by behavioral theory have been shown to be more effective in changing behaviors as compared to those that are not. The Theory of Planned Behavior (TPB) has been used to successfully predict and explain a variety of health related behaviors, including sugar-sweetened beverage (SSB) intake and physical activity (PA). The TPB assumes that behavioral intentions are the most important determinant of behavior. Intentions are the function of individual's attitudes toward the behavior (these evaluations can be positive or negative), subjective norms (social standards and expectations surrounding the behavior), and perceived behavioral control (perception of the ease with which the behavior can be performed). According to literature, behavioral intentions predict 20% - 40% of the variance in health behaviors with attitudes beings the strongest predictor of diet, and perceived behavioral control being the strongest predictor of physical activity related intentions. Excessive SSB consumption and inadequate PA have been highly associated with the obesity epidemic, and related comorbidities such as cardiovascular disease and type-2 diabetes. Understanding and targeting these behaviors through application of health behavior theories, such as the TPB, is important. PRIMARY AIMS: This research is embedded within a larger 2-arm randomized-control trial, Talking Health, which targets residents in rural southwest Virginia. Guided by the TPB, the overall goal of the Talking Health trial is to determine the effectiveness of a 6-month intervention aimed at decreasing SSB intake (SIPsmartER) compared to a matched contact control aimed at increasing PA (MoveMore). Each condition includes three classes, one teach-back call, and 11 interactive voice response (IVR) calls. The primary aims of this secondary analysis of Talking Health are to 1) determine if single-item TPB indicators are correlated with multi-item TPB scales for SSB and PA; 2) examine how baseline TPB variables predict participation in the SIPsmartER and MoveMore; 3) determine how the IVR TPB variables assessed during IVR calls predict future SSB and PA behaviors reported in a subsequent IVR call; and 4) explore how TPB variables change over the course of the teach back and 11 IVR calls. METHODS: Eligibility requirements included being 18 years of age or older, having reliable access to a telephone, drinking 200 kilocalories of SSB per day, and having no contraindications for moderate-intensity physical activity. The present research utilizes data from the baseline health assessment, class attendance and IVR and teach back calls completion data, as well as data collected in teach-back and 11 IVR calls. Multi-item TPB constructs for both SSB and PA behaviors were assessed at baseline (measured on a 7-point Likert scale). Each IVR call assessed self-reported past week behavior (ounces of SSB or minutes of PA) and four single-item TPB constructs including behavioral intentions, perceived behavioral control, instrumental attitudes, affective attitudes, and subjective norms. Participation was measured as the number out of 15 activities completed by participants (three classes, one teach back call, and 11 IVR calls). Statistical analysis included descriptive statistics, Chi square tests, independent T-tests, Pearson's correlations, Cronbach's α, and sequential multi-step regression models. Multiple data imputations were used to account for missing data. RESULTS: Of the 301 participants, 81% were female and 93% were Caucasian. The mean age of participants was 48.8 ± 13.5. Additionally, 32% of participants completed high school education, 55% earned < $20,000 per year, 32% had a full time or part time job, and 33% were classified as low health literate. Single-item indicators for both SSB-TPB questions (r > 0.60) and PA-TPB questions (r > 0.69) were highly correlated with their multi-item scales. Baseline TPB variables did not predict the participation rates in either SIPsmartER (F=1.763, R2=0.057, P=0.124) or MoveMore (F=0.815, R2=0.028, P=0.541) conditions. Of the nine SIPsmartER IVR regression models, eight were significant, and the SSB-TPB variables predicted about 30% of the variance in SSB behavior. Of the nine MoveMore IVR regression models, all were significant, and the PA-TPB variables predicted about 20% of the variance in SSB behavior. In both conditions, the majority of variance was explained by behavioral intentions and the addition of other TPB variables (perceived behavioral control, instrumental attitudes, affective attitudes, and subjective norms) explained substantially less variance in the behaviors. There were no notable patterns of change in TPB variables over 11 IVR calls for either SIPsmartER or MoveMore participants. DISCUSSION: Our findings show that single-item indicators can be used as reliable measures of the TPB constructs. The TPB model did not show significant predictive value when it comes to participation in SIPsmartER or MoveMore. On the other hand, our findings show that TPB model explained about 30% (SSB) and about 20% (PA) of variance in behavior. Although significant changes in IVR TPB variables were found between the two time points in several instances for both SSB and PA behavior, there were no patterns of change over time. Based on our findings, assessing behavioral intentions as the goal behavior in each IVR call may be the most useful application of the TPB. Other TPB variables can be assessed using single-item indicators. / Master of Science

Four field experiments were conducted to examine situational determinants of alcohol consumption at university fraternity parties. Certain manipulated variables were relevant to the development of environmental strategies for predicting and decreasing excessive alcohol consumption and deterring alcohol impaired driving. When entering university-sanctioned parties, students were asked a few questions and then they received a drink container and a stick-on badge, each with an ID number. During these parties, individual drinking rates of beer and mixed drinks or beer only were monitored under varying environmental conditions. When exiting the party, students' blood alcohol concentrations (BACs) were obtained with a breathalizer. In Experiment 1, three types of beer (Budweiser, Bud Light, and LA) were available in kegs labeled "A", "B", and "C". At a second party the kegs were labeled according to beer content (i.e., Budweiser, Bud Light, and LA). Results showed significant differences in drink choice across parties, with Budweiser most preferred by males and females when kegs did not indicate beer content and Bud Light most preferred by males and females when the kegs were labeled according to brand. The low alcohol (LA) choice was only selected substantially when the kegs did not reflect beer content. In Experiment 2, students of legal—drinking age at each of two weekend fraternity parties chose one of two types of alcoholic beverages (beer or mixed drinks) to consume throughout the night. Unbeknownst to the partiers, at the first of these two parties the beer drinkers were served regular beer, whereas at the second party low alcohol beer was served. Drinking rates were similar for beer and mixed-drink consumption at both parties; at the party with LA beer, students‘ mean BAC when leaving the party was significantly lower for the beer drinkers (i.e., .026 for LA consumers vs. .063 for mixed-drink consumers) For Experiment 3, students of legal drinking age at a weekend fraternity party chose one of two types of alcoholic beverages (beer or mixed drinks) to drink throughout the night. Unbeknownst to the partiers, drinkers were randomly assigned to either a regular alcohol content or low alcohol content version of their preferred beverage type. The drinking rates were greater for beer drinkers than for partiers consuming mixed drinks. Drinking rates were similar for both the low and regular alcohol alternatives. However, exit BAC was significantly greater for those in the regular-alcohol conditions. In Experiment 4, the alcoholic beverages available to students of legal—drinking age at one party (i.e., mixed drinks and beer) were served either by bartenders or served by themselves. A test of the theory of reasoned action was conducted by attempting to predict the number of drinks consumed and exit BAC from measures of general and specific intentions obtained two weeks before the party and at the start of the party. Specific drinking intentions obtained at the start of the party predicted a significant amount of variance in exit BAC (R = .59). The manipulation of situational variables also accounted for a portion of the variance in the number of drinks consumed and exit BAC. One environmental determinant at this party was the nature of drink delivery (i.e., self-serve vs. bartender). Male and female beer drinkers assigned to the Self-Serve condition drank at a higher rate and consumed more of their preferred beverage type than did those drinkers served by a bartender, or by those consuming mixed drinks in the self-serve condition. This increase was highest for male partiers. Male and female mixed drink consumers assigned to the Self·Serve condition drank at the lowest rate and consumed the least amount of their preferred beverage type. / Ph. D.

Health literacy (HL) is defined as the degree to which individuals have the capacity to obtain, process, and understand basic health information and services needed to make appropriate health decisions. Low HL has been associated with poorer self-reported health status, inability to manage chronic conditions, and less use of preventive services. More research is needed to explore the relationship between HL and sugar-sweetened beverage (SSB) intake and physical activity (PA). Although numerous HL interventions are evident in the literature, those that test HL as a moderator of effectiveness are lacking. Additionally, it has been recognized that systems-level HL efforts are necessary to lessen the burden of low HL. This dissertation addresses these needs with three unique studies that took place within the rural, medically underserved southwest Virginia region. Study 1 was a secondary analysis that examined the relationship and responsiveness of the Stanford Leisure-Time Activity Categorical Item (L-Cat) and adapted Godin Leisure-Time Exercise Questionnaire (GLTEQ) and determined if baseline HL status moderated intervention effects. There was high agreement L-Cat and adapted GLTEQ for classifying individuals as meeting PA recommendations. Baseline HL status did not moderate change in L-Cat or adapted GLTEQ measures. Study 2 was a secondary analysis that determined if 6-month change in SSB intake predicted 6-month change in body mass index (BMI), weight, and quality of life (QOL), and determined if HL moderated these relationships. The regression models for weight and QOL were not significant. The BMI model was significant. Six-month change in SSB intake, experimental condition, and age were significant predictors for the BMI model. As hypothesized, HL did not moderate relationships in any models. Study 3 details a multilevel mixed-methods needs assessment and collaboratively developed organizational HL improvement plan within the Virginia Department of Health (VDH). Staff responses revealed about half reported doing well across HL domains (written communication, oral communication, self-management and empowerment, supportive systems). However, needs were observed across all domains, with most improvement needed in written communication domain. There were significant correlations between clients' HL status and their perceptions of VDHs' HL practices, indicating potential areas of improvement within VDH. / Ph. D. / Health literacy is defined as the degree to which individuals have the capacity to obtain, process, and understand basic health information and services needed to make appropriate health decisions. Low health literacy has been associated with poorer self-reported health status, inability to manage chronic conditions, and less use of preventive services. More research is needed to explore the relationship between health literacy and sugar-sweetened beverage intake and physical activity. Specifically, interventions that test the moderation of health literacy (i.e., how health literacy affects the strength of a relationship) are needed. Additionally, it has been recognized that systems-level health literacy efforts are necessary to lessen the burden of low health literacy. This dissertation addresses these needs within the rural, medically underserved southwest Virginia region. Study 1 aimed to test the utility of two physical activity measures (Stanford Leisure-Time Activity Categorical Item and adapted Godin Leisure-Time Exercise Questionnaire) with a population of varying health literacy status with hopes of coming closer to identifying pragmatic standardized assessment of and detection of clinically meaningful change in physical activity behaviors, and to determine if health literacy moderated this change. Study 2 expanded upon existing research that has determined that a decrease in sugar-sweetened beverage intake can result in a decrease in weight and body mass index (BMI), and improvement in quality of life (QOL) by aiming to determine if six-month change in SSB intake predicted six-month change in BMI, weight, and QOL and if health literacy moderated these relationships. Study 3 responds to national and state-wide initiatives by detailing the first use of the AHRQ Toolkit within a public health setting, Virginia Department of Health, to conduct a needs assessment and organizational health literacy improvement plan.

Resistance exercise (RE) training is a well-known and effective method for promoting increases in muscle mass and strength. A single bout of RE induces physiological disturbances that require coordinated activation of the immune system and intramuscular signaling in order to return the tissue to homeostasis and adapt to the RE challenge. On the other hand, acute binge alcohol consumption can affect the immune response to an inflammatory challenge, intramuscular anabolic signaling, and muscle protein synthesis, and the effects of alcohol on these processes are opposite that of RE. Furthermore, individuals who report more frequent exercise also report a greater frequency of binge drinking. However, few investigations exist regarding the effects of binge alcohol consumed after a bout of RE on RE-induced physiological changes and performance recovery. Therefore, the overarching purpose of the investigations contained within this dissertation was to investigate the effect of alcohol consumed after RE on the RE-induced changes in mTOR pathway signaling, muscle protein synthesis, inflammatory capacity, strength recovery, and power recovery. Although RE increased mTOR pathway signaling and inflammatory capacity after exercise and reduced maximal strength and explosive power the day after exercise, we observed no effects of alcohol (1.09 g ethanol∙kg-1 lean body mass, designed to result in a peak blood alcohol concentration of approximately 0.12 g∙dl-1) consumed after RE on mTOR pathway signaling, 24-hour rates of muscle protein synthesis, inflammatory capacity, or strength and power recovery in resistance-trained individuals.

Achocolatados e bebidas achocolatadas são alimentos muito consumidos em praticamente todos os países, especialmente por crianças e jovens. Isso porque o chocolate é também reconhecido como um agente flavorizante largamente aceito. O objetivo geral deste trabalho foi elaborar um estudo de achocolatados e de bebidas achocolatadas disponíveis no mercado brasileiro, desenvolvendo-se técnicas de análises e obtendo-se resultados com finalidades comparativas. Ainda formulou-se bebida achocolatada com características funcionais, utilizando-se para tal quitosana modificada desenvolvida no Departamento de Tecnologia Bioquímico-Farmacêutica. Este trabalho foi dividido em duas partes, para melhor leitura e compreensão: parte 1: Achocolatados e parte 2: Bebidas achocolatadas. Para os achocolatados foram realizadas análises em produtos disponíveis no mercado brasileiro, como: composição centesimal, teobromina, alcalóides totais e pH. Procurou-se enfatizar as diferenças entre os produtos para finalidades especiais (\"diet\" e \"light\") e os tradicionais. Ainda, estudou-se propriedades tisicas, como força de compactação, densidade e granulometria, observando a correlação entre estas. Para bebidas achocolatadas, desenvolveu-se produto com quitosana modificada, atribuindo ao produto características funcionais. Estudou-se a reologia deste produto e de outros obtidos no mercado brasileiro, avaliando-se assim o produto desenvolvido. O teor de sólidos solúveis avaliado nestas amostras, com resultados muito próximos, sugeriu certa padronização na adição de leite, açúcar e sólidos de cacau em suas formulações. Para a bebida desenvolvida, aplicou-se teste sensorial ( aceitação global e viscosidade), utilizando-se como comparativo bebida do mercado brasileiro (Chocomilk), escolhida por ser comercializada em embalagem de vidro (semelhante à do produto em estudo). A aceitação global não demonstrou diferença significativa (P>0,05) entre as amostras, porém a característica mais encorpada da bebida desenvolvida apontou maior tendência de aceitação neste parâmetro. / Chocolate drink powders and chocolate beverages are very consumed foods in almost every contry, specially by children and young people. That is because chocolate is also recognized as a flavour agent greatly accepted. The aim of this work was to elaborate a study of chocolate drink: powders and chocolate beverages from Brazilian market, developing analytical techniques and obtaining results with comparatives purposes. It was developed chocolate beverage with functional characteristics, using for that modified chitosan, developed in this Department. This work was divided into two parts to a better reading and understanding: part 1: Chocolate drink: powders and part 2: Chocolate beverages. In the first part, some chemical analysis were carried out in the products from Brazilian market, such as lipids, proteins ashes, moisture, theobromine and total alkaloids content and the pH. It was emphasized the differences between diet, light and the standard products; The theobromine and total alkaloids content was used as an indicative of the differences. Some physical analysis in the chocolate drink powders were carried out, such as compaction of powder, particle size distribution and poured density of powder. In the second part, it was carried out rheological analysis of the products from the market. It was developed chocolate beverage with modified chitosan as a thickening agent, what gave it a functional characteristic. The rheological characteristics of the developed product were similar to that of the products from the market. Soluble solids analysis was carried out, also making a comparison between the developed product and the products from the market. The results were very similar, showing a standardization of the quantity of sugar, milk and cocoa solids in their formulation. Besides that, it was carried out sensory analysis, with an acceptance test of the developed product and the beverage chosen for comparison - Chocomilk. With the marks of acceptance test (global acceptance and viscosity) it was made a comparison between the two samples. It was noticed that the developed sample was more accepted than the other one, talking about the viscosity, and in talking about the global acceptance, there wasn\'t significant difference (P>0,05) between the samples.

Achocolatados e bebidas achocolatadas são alimentos muito consumidos em praticamente todos os países, especialmente por crianças e jovens. Isso porque o chocolate é também reconhecido como um agente flavorizante largamente aceito. O objetivo geral deste trabalho foi elaborar um estudo de achocolatados e de bebidas achocolatadas disponíveis no mercado brasileiro, desenvolvendo-se técnicas de análises e obtendo-se resultados com finalidades comparativas. Ainda formulou-se bebida achocolatada com características funcionais, utilizando-se para tal quitosana modificada desenvolvida no Departamento de Tecnologia Bioquímico-Farmacêutica. Este trabalho foi dividido em duas partes, para melhor leitura e compreensão: parte 1: Achocolatados e parte 2: Bebidas achocolatadas. Para os achocolatados foram realizadas análises em produtos disponíveis no mercado brasileiro, como: composição centesimal, teobromina, alcalóides totais e pH. Procurou-se enfatizar as diferenças entre os produtos para finalidades especiais (\"diet\" e \"light\") e os tradicionais. Ainda, estudou-se propriedades tisicas, como força de compactação, densidade e granulometria, observando a correlação entre estas. Para bebidas achocolatadas, desenvolveu-se produto com quitosana modificada, atribuindo ao produto características funcionais. Estudou-se a reologia deste produto e de outros obtidos no mercado brasileiro, avaliando-se assim o produto desenvolvido. O teor de sólidos solúveis avaliado nestas amostras, com resultados muito próximos, sugeriu certa padronização na adição de leite, açúcar e sólidos de cacau em suas formulações. Para a bebida desenvolvida, aplicou-se teste sensorial ( aceitação global e viscosidade), utilizando-se como comparativo bebida do mercado brasileiro (Chocomilk), escolhida por ser comercializada em embalagem de vidro (semelhante à do produto em estudo). A aceitação global não demonstrou diferença significativa (P>0,05) entre as amostras, porém a característica mais encorpada da bebida desenvolvida apontou maior tendência de aceitação neste parâmetro. / Chocolate drink powders and chocolate beverages are very consumed foods in almost every contry, specially by children and young people. That is because chocolate is also recognized as a flavour agent greatly accepted. The aim of this work was to elaborate a study of chocolate drink: powders and chocolate beverages from Brazilian market, developing analytical techniques and obtaining results with comparatives purposes. It was developed chocolate beverage with functional characteristics, using for that modified chitosan, developed in this Department. This work was divided into two parts to a better reading and understanding: part 1: Chocolate drink: powders and part 2: Chocolate beverages. In the first part, some chemical analysis were carried out in the products from Brazilian market, such as lipids, proteins ashes, moisture, theobromine and total alkaloids content and the pH. It was emphasized the differences between diet, light and the standard products; The theobromine and total alkaloids content was used as an indicative of the differences. Some physical analysis in the chocolate drink powders were carried out, such as compaction of powder, particle size distribution and poured density of powder. In the second part, it was carried out rheological analysis of the products from the market. It was developed chocolate beverage with modified chitosan as a thickening agent, what gave it a functional characteristic. The rheological characteristics of the developed product were similar to that of the products from the market. Soluble solids analysis was carried out, also making a comparison between the developed product and the products from the market. The results were very similar, showing a standardization of the quantity of sugar, milk and cocoa solids in their formulation. Besides that, it was carried out sensory analysis, with an acceptance test of the developed product and the beverage chosen for comparison - Chocomilk. With the marks of acceptance test (global acceptance and viscosity) it was made a comparison between the two samples. It was noticed that the developed sample was more accepted than the other one, talking about the viscosity, and in talking about the global acceptance, there wasn\'t significant difference (P>0,05) between the samples.

Leung, Yu Tim. / Thesis submitted in: November 2006. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references (leaves 103-128). / Abstracts in English and Chinese. / Thesis Committee --- p.i / Acknowledgements --- p.ii / Abstract --- p.iii / 摘要 --- p.iv / Table of Contents --- p.v / List of Tables --- p.ix / List of Figures --- p.x / Abbreviations --- p.xiii / Chapter 1. --- Introduction / Chapter 1.1 --- Flower herbal teas --- p.1 / Chapter 1.2 --- R. rugosa --- p.3 / Chapter 1.2.1 --- The phytochemistry of R. rugosa --- p.3 / Chapter 1.3 --- Secondary metabolites --- p.4 / Chapter 1.4 --- Classification of secondary metabolites --- p.6 / Chapter 1.5 --- Phenolic compounds --- p.6 / Chapter 1.5.1 --- Phenylpropanoid compounds --- p.6 / Chapter 1.5.2 --- Lignins --- p.7 / Chapter 1.5.3 --- Coumarins --- p.7 / Chapter 1.5.4 --- Stilbenes --- p.8 / Chapter 1.5.5 --- Tannins --- p.8 / Chapter 1.5.6 --- Flavonoids --- p.9 / Chapter 1.6 --- Oxidative Stress --- p.13 / Chapter 1.6.1 --- Diseases related to ROS --- p.13 / Chapter 1.6.2 --- Significant chemical or biochemical conversion of ROS --- p.14 / Chapter 1.6.3 --- Sources of ROS --- p.15 / Chapter 1.7 --- Natural dietary antioxidants --- p.15 / Chapter 1.7.1 --- Vitamin C --- p.15 / Chapter 1.7.2 --- Vitamin E --- p.16 / Chapter 1.7.3 --- Carotenoids --- p.16 / Chapter 1.7.4 --- Phenolic compounds --- p.16 / Chapter 1.8 --- Cancinogenesis --- p.17 / Chapter 1.9 --- Cell cycle --- p.18 / Chapter 1.9.1 --- Cell cycle of eukaryotic cells --- p.18 / Chapter 1.9.2 --- Checkpoints of cell cycle --- p.18 / Chapter 1.10 --- Cancer cell lines --- p.19 / Chapter 1.11 --- The growth phases of cancer cell lines --- p.20 / Chapter 1.12 --- Antiproliferative effects of phenolic compounds --- p.21 / Chapter 1.13 --- Genotoxicity of phenolic compounds --- p.22 / Chapter 1.14 --- Objectives --- p.23 / Chapter 2. --- Methods and Materials / Chapter 2.1 --- Extraction of active substances --- p.40 / Chapter 2.2 --- Determination of antioxidant activities TEAC assay --- p.40 / Chapter 2.3 --- Determination of hydroxy 1 radical scavenging activity by the deoxyribose assay --- p.41 / Chapter 2.4 --- Determination of phenolic contents by Folin´ؤCiocalteu assay --- p.43 / Chapter 2.5 --- Determination of total flavonoid by aluminum chloride colorimetric method --- p.43 / Chapter 2.6 --- Determination of oxidative DNA damage by comet assay --- p.44 / Chapter 2.7 --- Cell lines propagation --- p.49 / Chapter 2.8 --- Determination of antiproliferative activities by MTT assay (colorimetric) --- p.50 / Chapter 2.9 --- Determination of antiproliferative activities by BrdU labeling assay --- p.52 / Chapter 2.10 --- Cell cycle analysis by flow cytometry --- p.55 / Chapter 2.11 --- Determination of genotoxicity by SOS chromotest --- p.57 / Chapter 3. --- Results / Chapter 3.1 --- Dermination of antioxidant activities by TEAC assay --- p.59 / Chapter 3.1.1 --- Trolox Standard Reference --- p.59 / Chapter 3.1.2 --- TEAC of the seven flower extracts --- p.59 / Chapter 3.2 --- Hydroxyl radical scavenging activity by deoxyribose assay --- p.60 / Chapter 3.3 --- Determination of phenolic contents by Folin´ؤCiocalteu assay --- p.60 / Chapter 3.4 --- Determination of total flavonoids by colorimetirc aluminium chloride assay --- p.61 / Chapter 3.5 --- "The Inter-correlation between the antioxidant activities, total phenolic and flavonoid contents of flower extraction powders" --- p.61 / Chapter 3.6 --- Determination of oxidative DNA damage by comet assay --- p.62 / Chapter 3.7 --- Determination of antiproliferative activities by MTT assay --- p.63 / Chapter 3.7.1 --- Antiporoliferative activities on HepG2 --- p.63 / Chapter 3.7.2 --- Antiproliferative activities on MCF7 --- p.63 / Chapter 3.7.3 --- IC50 of R. rugosa extract on both HepG2 and MCF7 --- p.64 / Chapter 3.8 --- "The Inter-correlation between antioxidant activities, total phenolic contents, flavonoid contents, and the antiproliferative activities of flower extraction Powders" --- p.64 / Chapter 3.9 --- Determination of DNA synthesis by BrdU labeling analysis --- p.65 / Chapter 3.10 --- Cell cycle analysis by flow cytometry --- p.65 / Chapter 3.11 --- Determination of genotoxicity by SOS chromotest --- p.66 / Chapter 4. --- Discussions / Chapter 4.1 --- Extraction method --- p.90 / Chapter 4.2 --- Comparison of TEAC of the dry flowers with other foods --- p.90 / Chapter 4.3 --- Correlation between ABTS+ and hydroxyl scavenging ability of flower extraction powder --- p.91 / Chapter 4.4 --- Comparison of phenolic contents of the fry flowers with other foods --- p.92 / Chapter 4.5 --- Correlation between total phenolic contents and flavonoid contents of flower Eextraction powders --- p.92 / Chapter 4.6 --- "Correlation between total phenolic, flavonoid content and antioxidant activities of flower extraction powders" --- p.93 / Chapter 4.7 --- Factors affecting the antioxidant power besides total phenolic contents --- p.94 / Chapter 4.8 --- Synergistic effect of phenolic compounds --- p.94 / Chapter 4.9 --- Toxicity of drinking flower herbal tea --- p.95 / Chapter 4.10 --- Recommended dose of flower herbal teas --- p.96 / Chapter 4.11 --- Antiproliferative activities of flower extracts by MTT assay --- p.97 / Chapter 4.12 --- Antiproliferation activities of flower extraction Powders by Brdu labeling assay --- p.98 / Chapter 4.13 --- Protective effects of flower extraction powder on oxidative DNA damage determined by comet assay --- p.99 / Chapter 4.14 --- Cell cycle analysis --- p.100 / Chapter 4.15 --- Further Studies --- p.101 / Chapter 5. --- Conclusion --- p.102 / Chapter 6. --- References --- p.103

Através de Testes de Diluição em Sistema de Tubos Múltiplos determinou-se a Intensidade de Atividade de Inibição Bacteriana (IINIB/bacteriostasia) e a Intensidade de Atividade de Inativação Bacteriana (IINAB/bactericidia) de soluções conservantes contendo extrações etanólicas (alcoolatura/planta verde, hidroalcoolatura/planta seca) e hídricas (decoctos/planta verde ou seca) de Ocimum gratissimum L. (“alfavacão”, “alfavaca”, “alfavaca-cravo”) – Labiatae - (Lamiaceae), sobre inóculos padronizados de Salmonella Enteritidis (ATCC 11076), Escherichia coli (ATCC 11229) e Staphylococcus aureus (ATCC 25923). Testou-se também, a partir da formulação de quatro bebidas, duas alcoólicas e duas não alcoólicas, com e sem açúcar respectivamente, a partir de extrato reconstituido (alcoolatura/planta verde) desta planta, em diferentes concentrações (5%, 15% e 30%), através de Testes de Suspensão em Sistema de Tubos Múltiplos, a Intensidade de Atividade de Inativação Bacteriana (IINAB/bactericidia), sobre Salmonella Enteritidis (ATCC 11076), bem como a aceitabilidade/preferência sensorial por Escala Hedônica destes quatro produtos. Todas as diferentes formas de extração apresentaram capacidade de inibição e/ou inativação seletivas sobre os três inóculos bacterianos, tendo a forma de extração alcoólica apresentado a atividade antibacteriana mais intensa (inibição/inativação) frente aos três agentes. A bactéria mais sensível à atividade antibacteriana em todas as soluções conservantes foi Salmonella Enteritidis. Staphylococcus aureus apresentou a menor sensibilidade às formas de decocção, enquanto Escherichia coli apresentou a menor sensibilidade frente a forma de extração hidroalcoólica. Todas as formulações apresentaram atividade bactericida para Salmonella Enteritidis, diretamente proporcional às concentrações do extrato e ao tempo de exposição da bactéria às bebidas, destacando-se neste sentido a formulação não alcoólica com açúcar. Na análise sensorial a preferência aumentou com o decréscimo da concentração de extrato de Ocimum gratissimum na formulação. A bebida não alcoólica com açúcar, na concentração de 5% de extrato, destacou-se na preferência sensorial/aceitabilidade. / Through Dilution Tests in Multiple Tubes System, the intensity of bacterial inhibition activity (IINIB/bacteriostasys) and the intensity of bacterial inactivation activity (IINAB/bactericidie) from preservatives solutions containing etanolic extractions (alcoholature/green plant, hidroalcoholature/dry plant) and hydric extractions (decoctions/green or dry plant) of Ocimum gratissimum L. (African basil) - Labiatae - (Lamiaceae), on standardized inocula of Salmonella Enteritidis (ATCC 11076), Escherichia coli (ATCC 11229) and Staphylococcus aureus (ATCC 25923) was determined. In the same way, the Activity Intensity of Bacterial Inactivation (IINAB/bactericidie), in four drinks formulations, two alcoholic and two no-alcoholic, with and without sugar respectively, from reconstituted extract (alcoholature/green plant) of the same plant, at different concentrations (5%, 15% and 30%), on Salmonella Enteritidis (ATCC 11076) was determined by Suspension Tests in Multiple Tubes System, and the acceptability/sensory preference by Hedonic Scale of these four products. All differents extractions forms had showed selective capacity of inhibition or inactivation on the three bacterial inocula. The alcoholic extraction form presented the antibacterial activity more intense (inhibition/inactivation) against the three agents. The sensitivest bacteria by all preservatives solutions was Salmonella Enteritidis. Staphylococcus aureus showed a lower sensibility to the decoction types, while Escherichia coli showed a lower sensibility to the hydroalcoholic extraction. All food formulations showed bactericidal activity on Salmonella Enteritidis, directly proportional to the extract concentrations and the exposure time of the bacterium in beverages, pointing the not alcohol with sugar formulation. In the sensorial analysis the preference increased with the decrease of the extract concentration of Ocimum gratissimum. The non-alcoholic beverage with sugar, in the extract concentration of 5%, pointed in the sensorial preference// acceptability.

Siu, Kit Wah Lydia. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (p. 201-207). / Abstracts in English and Chinese. / Acknowledgements --- p.i / Chapter Chapter 1 --- Introduction --- p.1 / Aim of the Study --- p.1 / Background --- p.3 / Literature Review --- p.10 / """Conspicuous"" Wine Consumption" --- p.14 / Taste as a Combination of Symbols of Class Distinction --- p.17 / "Hong Kong, a City of Consumption" --- p.19 / Methodology --- p.21 / Challenges and Limitations --- p.25 / Summary of the Thesis --- p.26 / Chapter Chapter 2 --- The Wine Market in Hong Kong --- p.28 / Introduction --- p.28 / Consumption and Identity --- p.29 / The Beginning of Wine Consumption in Hong Kong --- p.33 / The Beginning of the Mass Consumption of Wine in the 1960s --- p.36 / Popularization in the 1980s to the Early 1990s --- p.39 / The Years of Turbulence 1997-2002 --- p.40 / The Current Wine Market --- p.44 / General Market Features --- p.46 / Conclusion --- p.48 / Chapter Chapter 3 --- The Wine-Tasting Community in Hong Kong --- p.51 / Introduction --- p.51 / The Meaning of Taste --- p.52 / Who are the People in the Wine Tasting Community? --- p.58 / Gender --- p.59 / Age --- p.60 / Level of Economic Capital --- p.61 / Education level --- p.62 / Four Types of Wine-Tasting Events --- p.64 / Large-scale Public Tasting Events --- p.64 / Medium-scale Tasting Events --- p.67 / Private Wine-tasting Events --- p.69 / Wine dinners --- p.70 / Conclusion --- p.72 / Chapter Chapter 4 --- The Rituals of Wine Tasting as Expressions of Identity --- p.75 / Introduction --- p.75 / "Sight, Aroma and Flavor" --- p.77 / The Practices of Tasting Rituals in Large-Scale Public Wine-Tasting Events. --- p.80 / Assessment of Sight --- p.84 / Assessment of Aroma --- p.85 / Assessment of Flavor --- p.87 / The Final Step: Spitting --- p.88 / Beyond Tasting: Wine Talk --- p.91 / Conclusion --- p.94 / Chapter Chapter 5 --- The Symbols of Social Distinction in Wine Consumption --- p.99 / Introduction --- p.99 / The Relationship of Wine and Social Distinction --- p.100 / Westernness --- p.102 / Uniqueness --- p.105 / Wealth --- p.107 / Knowledge --- p.112 / Leisure --- p.118 / Art and Aestheticism --- p.121 / Health Consciousness --- p.123 / Conclusion --- p.127 / Chapter Chapter 6 --- Wine as Individual Identity --- p.131 / Introduction --- p.131 / Brief Description of YC and its wine group --- p.133 / Setting --- p.133 / Becoming a member of YC --- p.137 / The structure of the YC wine group --- p.139 / The Core Value: Tasting vs Drinking --- p.143 / Taboos in the YC Wine Group --- p.144 / The Role of Alcohol and Intoxication --- p.145 / Demeaning Wine's Taste --- p.149 / Learning about Taste: Hierarchical or Equal? --- p.154 / Objective Evaluation.. --- p.157 / Subjective Appreciation --- p.160 / Searching for Oneself: a Favorite Wine and a Preferred Taste --- p.165 / Conclusion --- p.171 / Chapter Chapter 7 --- Comparing San Francisco and Hong Kong as to Wine --- p.174 / Introduction --- p.174 / Fieldwork in San Francisco --- p.175 / Drinkers´ة society vs Tasters' society --- p.176 / "Drinkers, Tasters and Producers" --- p.179 / Learning to Taste Wine in San Francisco --- p.182 / The Understanding of Wine and Taste in San Francicso --- p.185 / Chapter Chapter 8 --- Conclusion --- p.192 / Chapter Appendix: --- Reference --- p.201