Myocilin, expressed in the trabecular meshwork of the eye, has been linked to inherited primary open-angle glaucoma (POAG). The biological function of myocilin is unknown, but mutant myocilin exhibits a gain-of-function mechanism, aggregating within the endoplasmic reticulum of human trabecular meshwork cells, causing cell stress and eventually apoptosis. After apoptosis occurs, the trabecular meshwork is compromised, leading to an increase in intraocular pressure, a symptom of glaucoma. In this thesis, I have expressed and purified the wild-type olfactomedin (OLF) domain and 24 reported disease-causing variants. I developed a facile thermal stability assay using differential scanning fluorimetry, which follows the unfolding of a protein through the fluorescence of a dye sensitive to hydrophobic regions of a protein. Also in this thesis I have determined melting temperatures for the wild-type and for each of the disease-causing mutants. I have tested the stability of the mutants in the presence of seven osmolytes, with sarcosine and trimethylamine-N-oxide restoring the melting temperature closest to wild-type. Additionally, I expressed and purified three reported single nucleotide polymorphisms (SNPs) (E352Q, E396D, K398R), which are considered benign variants. Variants were also compared by circular dichroism, revealing high b-sheet content and wild-type structure. When compared to previous studies, there is a positive correlation between the melting temperature, and previously reported qualitative assays, which measure the mutant myocilin solubility in detergent, secretion from mammalian cells, and aggregation propensity. Taken together, these data give insight into the relationship between glaucoma genotypes and phenotypes.

<p>Banners är annonser som finns på de flesta webbsidor. Användare är oftast ute på Internet med syftet att leta efter specifik information. Vid informationsökning används troligen ett speciellt kognitivt schema som gör att användaren undviker att fokusera på banners eftersom informationen som eftersöks inte antas finnas i banners. Om en navigering på Internet görs för lik en banner ser inte användaren den heller. Man kan säga att banner blindness är en tendens hos användaren av webben att ignorera banners, även när de innehåller information som användaren aktivt söker. Detta examensarbete undersöker om banners lättare kan kommas ihåg när ämnet överensstämmer med webbsidans text. En experimentell studie genomfördes med två betingelser, en grupp fick kontextberoende och den andra fick kontextoberoende banners. Medelvärdena visade att det var lättare att komma ihåg kontextoberoende banners, men inget signifikant säkerställt resultat erhölls. Resultatet kan ha påverkats av att det var få försöksdeltagare</p>

This study explored the experience of disability as recounted by school aged, visually impaired adolescents. The primary aim was to explore the manner in which these adolescents thought about, understood and coped with their disability. A second aim explored the extent to which participants’ experiences mirrored those reported in the literature. The design of the study was qualitative with an orientation toward social constructionism. Nine partially sighted and seven blind adolescents comprised the two cohorts of participants who participated in the study. One of the most notable findings supported the argument that disability is a socially constructed phenomenon. Some insights, drawn from psychoanalysis, were also found to be useful. Other findings, a critique of the study, and suggestions for future research are also provided. One of the most important of these involves evaluating the negative and positive consequences of inclusive education.

Abstract The current study evaluated the effects of training on the haptic classification of culturally universal facial expressions of emotion as depicted in simple 2D raised-line drawings. Blindfolded sighted (N = 60) and blind (N = 4) participants participated in Experiments 1 and 3, respectively. A small vision control study (N = 12) was also conducted (Experiment 2) to compare haptic versus visual learning patterns. A hybrid learning paradigm consisting of pre/post- and old/new-training procedures was used to address the nature of the underlying learning process in terms of token-specific learning and/or generalization. During the Pre-Training phase, participants were tested on their ability to classify facial expressions of emotion using the set with which they would be subsequently trained. During the Post-Training phase, they were tested with the training set (Old) intermixed with a completely novel set (New). For sighted observers, visual classification was more accurate than haptic classification; in addition, two of the three adventitiously blind individuals tended to be at least as accurate as the sighted haptic group. All three groups showed similar learning patterns across the learning stages of the experiment: accuracy improved substantially with training; however, while classification accuracy for the Old set remained high during the Post-Training test stage, learning effects for novel (New) drawings were reduced, if present at all. These results imply that learning by the sighted was largely token-specific for both haptic and visual classification. Additional results from a limited number of blind subjects tentatively suggest that the accuracy with which facial expressions of emotion are classified is not impaired when visual loss occurs later in life. / Thesis (Master, Neuroscience Studies) -- Queen's University, 2009-10-23 12:04:41.133

It is practically impossible to peruse any discussion relating to psychology or philosophy without encountering frequent references to sense deprivation. These usually take the form of supposititious analogies regarding the world of the blind and the deaf, and, in many cases such analogies are entirely incorrect . It is the aim of the present work to place the aberrations from what is commonly accepted as normal mental development which accompany the loss of vision or hearing, in a more scientific and unprejudiced light than that in which they have hitherto been considered. The writer himself with vision so seriously defective as to place him well within t he scope of the common definition applied to blindness , recently became interested i n the purely psychological aspects of sense deprivation . It occurred to him that psychology as a science ought to be able to contribute toward the solution of the educational and social problems of the blind and the deaf, and he believes that the material incorporated in the following chapters is sufficient to justify this hypothesis. [...]

The visual cortex of human observers changes its functionality in response to visual deprivation (Boroojerdi et al., 2000). Behavioral studies have recently documented enhanced tactile abilities following a short period of visual deprivation (Facchini & Aglioti, 2003; Weisser, Stilla, Peltier, Hu, & Sathian, 2005). The current study investigated the effects of visual deprivation on two unique tactile tasks. While Facchini and Aglioti observed significant effects of visual deprivation, neither Wong, Hackeman, Hurd, and Goldreich (2011) nor Merabet et al. (2008) observed these effects. Corroborating these more recent results, no difference in grating orientation discrimination performance was observed between the sighted and visually deprived participants in the first experiment. A significant effect of experience was seen in both groups, however, irrespective of the deprivation period of 90 minutes. The second experiment immediately followed the conclusion of the first experiment. Using the same stimuli and procedures from past experiments (Norman, Clayton, Norman, & Crabtree, 2008), it investigated the participants’ haptic discrimination of 3-dimensional object shape. Again, no significant difference in performance was found between the sighted and visually deprived participants. Together, the current results show that a brief period of visual deprivation (1.5 hours) produces no significant behavioral changes for these tactile and haptic tasks.

To date, the subject of disability has been highly disregarded in Germany’s academia. In the field of Arts, the topic is a derivative. This thesis makes a contribution to the discipline of Disability Studies and approaches the problematic from the field of German Studies. The analysis focuses on the sensory disabilities deafness and blindness and its depiction in the movies Jenseits der Stille und Erbsen auf Halb Sechs. The goal of this paper is to attract notice to the ways of representation of disability in German media in general. On the one hand, film reflects generally accepted public attitudes. But since film is a mass media, it has the potency to alter and question the communal opinion on the other hand. Thus, it can change society’s mindset about people with disability. Furthermore, this paper points out the different starting points for further liberal arts oriented approaches for investigations of disability in the media. However, the film analysis is based on two theses. The first one claims that the focus in the representation of people with disability does not concentrate on the human beings. On the contrary, it rather concentrates on the disability itself and the consequences that spring of it. The second thesis pursues this thought and says that the movies not only focus on the depiction of disability. In fact the disabled figures define themselves by their handicap and, in return, are defined by their physical aberrance by their social surroundings, which is foremost not disabled. Thereby, the disability forms an almost insuperable interpersonal obstacle. The analysis of the two movies is done along three research questions. The first one concerns the connection of physical handicap and the way people with disability think and act in relation to their surroundings and life in general. The second question is what factors, like gender, social class, or age, are crucial in self- and external perception, and therefore, in the formation of identity. The last investigation concentrates on the special abilities of people with disability, and scrutinizes if the aptitudes serve as compensations for their disabilities. The basis of this paper is Michel Foucault’s theory of mechanisms of societal exclusion, and the construction of categories such as norm and abnormity. Especially his works Wahnsinn und Gesellschaft, Überwachen und Strafen and his lecture course of Die Anormalen are essential in this perspective. In addition, the cultural and social currents in the disability studies serve as fundamental approaches. These two theories help to identify the vague term disability, and consequently, the object of investigation in this paper. In summary, the analysis of Jenseits der Stille and Erbsen auf Halb Sechs shows that the representation of disabled people in German film is predominantly stereotypic. Therein, disablement means great misfortune, and the loss of the central position in discourse. The disabled figures are socially alienated from their non-disabled surroundings. The isolation, however, is brought about by discursive mechanisms such as the power of medicine. In both movies, the disabled and non-disabled people are focused on the disability in their self and external perception. Accordingly, disability is linked to the feeling of shame, a lack of acceptance and the stigmatization of deviants. Self-acceptance can only be accomplished outside of the discourse of the normal, in which disabled are stigmatized. This paper reveals that the representation of people with disability in German film still shows a rather stereotypic image. Therefore, they tend to confirm societal prejudices rather than to challenge them.

Visual information about the form of an object and its movement in the world can be processed independently. These processing streams must be combined, since our visual experience is of a unitary stream of information. Studies of interactions between motion and form processing can therefore provide insight into how this combination occurs. The present thesis explored two such interactions between motion and spatial coding in human vision. The title of the thesis, “Pencils and Erasers”, serves as an analogy for the thesis’ principal findings. I investigate one situation in which moving patterns can impair the visibility of stationary forms, and another in which the visibility of form is enhanced by motion. In motion-induced blindness (MIB; Bonneh, Cooperman, & Sagi, 2001), salient stationary objects can seem to disappear intermittently from awareness when surrounded by moving features. Static forms proximate to motion can be “erased” from awareness. The thesis contributes to the answer to a simple question: why does MIB happen? My interpretation of this phenomenon emphasises the possible functional benefit of such an eraser around moving form: to suppress artifacts of visual processing from awareness. Chapter 2 demonstrates that motion per se is not required for MIB (Wallis & Arnold, 2008). MIB depends on the rate of luminance change over time, rather than the velocity (or change in position) of the inducing mask. MIB can therefore be characterised as a temporal inhibition, which does not critically depend on direction selective (motion) mechanisms. A similar mechanism of temporal inhibition that does not depend on motion is that which suppresses motion streaks from awareness. The human visual system integrates information over time. Consequently, moving image features produce smeared signals, or “motion streaks”, much like photographing a moving object using a slow shutter speed. We do not experience motion streaks as much as might be expected as they are suppressed from awareness in most circumstances. Evidence suggests that this suppression is enacted by a process of local temporal inhibition, and does not depend on motion mechanisms – much like MIB. These similarities led us to propose that MIB and motion streak suppression might reflect the same mechanism. In the case of MIB, physically present static targets may not be differentiated from signals arising from within the visual system, such as a motion streak. Chapter 3 of the thesis presents four converging lines of evidence in support of this hypothesis (Wallis & Arnold, 2009). The link between MIB and a mechanism of temporal inhibition that serves to suppress motion streaks is further strengthened by a recent report from our laboratory of a new visual illusion, Spatio-Temporal Rivalry (STR; Arnold, Erskine, Roseboom, & Wallis, in press), that is included in the present thesis as an appendix. Why does MIB occur? I suggest that at its base level, MIB reflects the activity of this simple visual mechanism of temporal inhibition (see Gorea & Caetta, 2009). This mechanism might usually serve a functional role in everyday vision: for example, by suppressing the perception of motion streaks. The second motion and form interaction investigated in the thesis represents a situation in which motion can improve form sensitivity. In some situations, observing a moving pattern can objectively improve sensitivity to that pattern after the offset of motion. The visual system can “pencil in”, or improve the visibility of, subsequent visual input. When a form defined by its motion relative to the background ceases to move, it does not seem to instantly disappear. Instead, the form is perceived to remain segregated from the background for a short period, before slowly fading. It is possible that this percept represents a consequence of bias or expectation, not a modulation of static form visibility by motion. Contrary to this possibility, Wallis, Williams and Arnold (2009) demonstrate that alignment sensitivity to spatial forms is improved by pre-exposure to moving forms (Chapter 4). I suggest that the subjective persistence of forms after motion offset and this spatial facilitation may represent two consequences of the same signal. The experiments herein address one situation in which moving patterns can impair the visibility of stationary forms and one in which moving patterns enhance the visibility of stationary forms. Therefore, the present thesis characterises two interactions between form and motion processing in human vision. These mechanisms of “pencil” and “eraser” facilitate the clear perception of objects in our visual world.

Thesis (Ph.D.)--University of Melbourne, The National Vision Research Institute of Australia and Dept. of Optometry & Vision Sciences, 2007. / Typescript. Includes bibliographical references (leaves 95-111).

Thesis (Ph. D.)--Duke University, 2007.