Data de aprova??o ausente. / Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2017-02-13T18:51:06Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) tatiana_fernandes_araujo_almeida.pdf: 2531556 bytes, checksum: 11a2583c0414dc55d6f907a2566a4de5 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2017-03-06T12:21:52Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) tatiana_fernandes_araujo_almeida.pdf: 2531556 bytes, checksum: 11a2583c0414dc55d6f907a2566a4de5 (MD5) / Made available in DSpace on 2017-03-06T12:21:52Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) tatiana_fernandes_araujo_almeida.pdf: 2531556 bytes, checksum: 11a2583c0414dc55d6f907a2566a4de5 (MD5) Previous issue date: 2016 / O carcinoma espinocelular oral (CECO) ? uma neoplasia maligna que acomete principalmente idosos. O desencadeamento da doen?a ? relacionado ? exposi??o aos fatores de risco e ao decl?nio da fun??o imune associada ao envelhecimento cronol?gico. Alguns estudos t?m demonstrado um aumento na incid?ncia de CECO em adultos jovens, sugerindo envolvimento de outros fatores na etiologia da doen?a, como uma altera??o do sistema imunol?gico. As c?lulas dendr?ticas (CDs) s?o c?lulas apresentadoras de ant?geno profissionais e estimuladoras eficazes para a expans?o clonal de linf?citos. Acredita-se que uma altera??o da fun??o das CDs em pacientes com c?ncer contribui para a falha da resposta antitumoral, levando a uma consequente progress?o da doen?a. O objetivo deste estudo foi avaliar, atrav?s da imunoistoqu?mica, se h? diferen?a na quantifica??o tissular dos subgrupos de CDs, associada ? idade, em esp?cimes de bi?psias de CECO. Para isso, foram selecionados casos de CECO de pacientes em tr?s diferentes faixas et?rias: G1 (< 40 anos de idade, n = 12), G2 (? 40 at? < 60 anos de idade, n = 15) e G3 (? 60 anos de idade, n = 14). Os marcadores utilizados foram S100, CD1a, CD207 (para CDs imaturas, imCDs), CD83 e CD208 (para CDs maduras, mCDs). As imagens foram capturadas dos campos com maior intensidade de marca??o nas l?minas histol?gicas e a quantifica??o celular foi realizada com o aux?lio do software Image J. A frequ?ncia e localiza??o das CDs foram avaliadas e analizadas estatisticamente nas regi?es intratumoral (intertumoral e ou estromal) e extratumoral. No geral, imCDs foram significativamente mais frequentes que mCDs em todos os grupos. ImCDs e mCDs mostraram preferencialmente localiza??o intratumoral e extratumoral respectivamente. Comparando G1 versus G2 / G3 foi observada um significativo menor n?mero de mCDs em G1. Na compara??o de G1 em rela??o a G2 ou G3 houve um n?mero significativamente menor de ambas, imCDs e mCDs. Nossos resultados mostram que existe uma menor quantidade de imCDs e mCDs em CECO afetando pacientes jovens em compara??o com idosos, sugerindo um comprometimento da resposta imune antitumoral em G1e permitindo a progress?o do tumor. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Odontologia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, [2016]. / Oral squamous cell carcinoma (OSCC) is a malignant neoplasm that mainly affects elderly people. The onset of the disease is related with exposure to risk factors and the decline of immune function associated with chronological aging. Previous studies have demonstrated an increased incidence of CECO in young adults, suggesting involvement of other factors in the etiology of the disease, such as a change in the immune system. Dendritic cells (DCs) are professional antigen presenting cells and effective for stimulating clonal expansion of lymphocytes. It is believed that a change in the function of DC in cancer patients contributes to the failure of the anti-tumor response, leading to a consequent progression of the disease. The aim of this study was to evaluate, by immunohistochemistry, if there were differences in tissue quantification of DCs subsets, associated with age in specimens of OSCC biopsies. For this OSCC cases were selected at three different ages: G1 (< 40 years old, n = 12), G2 (? 40 to <60 years old, n = 15) and G3 (? 60 years old, n = 14). The markers used were S100, CD1a, CD207 (for immature DCs, imDCs), CD83 and CD208 (for mature DCs, mDCs). Images were captured from fields with higher intensity staining in histological sections and cell quantification was performed with Image J software help. The frequency and localization of immunostained DCs were analyzed in intratumoral (intranestal and/or extranestal) and extratumoral areas and statistically compared. Overall, imDCs than mDCs were significantly more frequent in all groups. ImDCs and mDCs showed preferential intratumoral and extratumoral localization, respectivel. Comparing G1 than G2/G3 showed a significant lesser number of mDCs. G1 versus G2 or G3 there is a significantly lower number of both imDCs and mDCs. Our results show a lower number of both imDCs and mDCs in OSCC affecting younger than elderly patients, suggesting impairment of an effective antitumor immune response in G1 and enabling tumor progression, showing a gradual establishment of the antitumor immune response mediated by DCs according to the age, but with defects in quality.

Made available in DSpace on 2014-12-17T14:13:34Z (GMT). No. of bitstreams: 1 PauloRMA_TESE.pdf: 401951 bytes, checksum: 67a77886525e21487c037cd2f95939a0 (MD5) Previous issue date: 2010-05-07 / Introduction: Mouth cancer is classified as having one of the ten highest cancer incidences in the world. In Brazil, the incidence and mortality rates of oral cancer are among the highest in the world. Intraoral cancer (tongue, gum, floor of the mouth, and other non-specified parts of the mouth), the accumulated survival rate after five years is less than 50%. Objectives: Estimate the accumulated survival probability after five years and adjust the Cox regression model for mouth and oropharyngeal cancers, according to age range, sex, morphology, and location, for the city of Natal. Describe the mortality and incidence coefficients of oral and oropharyngeal cancer and their tendencies in the city of Natal, between 1980 and 2001 and between 1997 and 2001, respectively. Methods: Survival data of patients registered between 1997 and 2001 was obtained from the Population-based Cancer Record of Natal. Differences between the survival curves were tested using the log-rank test. The Cox proportional risk model was used to estimate risk ratios. The simple linear regression model was used for tendency analyses of the mortality and incidence coefficients. Results: The probability after five years was 22.9%. The patients with undifferentiated malignant neoplasia were 4.7 times more at risk of dying than those with epidermoid carcinoma, whereas the patients with oropharyngeal cancer had 2.0 times more at risk of dying than those with mouth cancer. The mouth cancer mortality and incidence coefficients for Natal were 4.3 and 2.9 per 100 000 inhabitants, respectively. The oropharyngeal cancer mortality and incidence coefficients were, respectively, 1.1 and 0.7 per 100 000 87 inhabitants. Conclusions: A low survival rate after five years was identified. Patients with oropharyngeal cancer had a greater risk of dying, independent of the factors considered in this study. Also independent of other factors, undifferentiated malignant neoplasia posed a greater risk of death. The magnitudes of the incidence coefficients found are not considered elevated, whereas the magnitudes of the mortality coefficients are high / Introdu??o: O c?ncer de boca ? classificado como uma das dez maiores incid?ncias de c?ncer no mundo. No Brasil, as taxas de incid?ncia e de mortalidade por esse c?ncer encontram-se entre as mais elevadas do mundo. Para o c?ncer intraoral (l?ngua, gengiva, base da boca e outras e n?o especificadas partes da boca), a taxa acumulada de sobrevida ap?s 5 anos ? menor que 50%. Objetivo: Estimar a probabilidade acumulada de sobrevida ap?s 5 anos, ajustar o modelo de regress?o de Cox para os c?nceres de boca e de orofaringe, segundo faixa et?ria, sexo, morfologia e localiza??o, para a cidade de Natal, Brasil. Descrever os coeficientes de mortalidade e de incid?ncia dos c?nceres de boca e de orofaringe e as tend?ncias desses coeficientes para a cidade de Natal, nos per?odos de 1980 a 2001 e de 1997 a 2001, respectivamente. Metodologia: Foi obtida a sobrevida de pacientes registrados entre 1997 e 2001 no Registro de C?ncer de Base populacional de Natal. Foram testadas as diferen?as entre as curvas de sobrevida atrav?s do teste log-rank. O modelo de riscos proporcionais de Cox foi utilizado para estimativas das raz?es de riscos. O modelo de regress?o linear simples foi utilizado para as an?lises de tend?ncia dos coeficientes de incid?ncia e de mortalidade. Resultados: A probabilidade acumulada ap?s 5 anos para todos os casos foi de 22,9%. Os pacientes com neoplasia maligna indiferenciada t?m 4,7 vezes mais risco de morrer do que aqueles com carcinoma epiderm?ide, enquanto que os pacientes com c?ncer de orofaringe t?m 2,0 vezes mais risco de morrer do que aqueles com c?ncer de boca. Os coeficientes padronizados de mortalidade e de incid?ncia do c?ncer de boca em Natal foram, respectivamente, 2,9 e 4,3 por 100 mil habitantes. Para o c?ncer de orofaringe 10 os coeficientes obtidos de mortalidade e de incid?ncia foram, respectivamente, 1,1 e 0,7 por 100 mil habitantes. Conclus?o: Identifica-se uma baixa taxa de sobrevida ap?s 5 anos. Pacientes com c?ncer de boca apresentam menos risco de morte, independentemente dos fatores considerados neste estudo. Tamb?m de forma independente dos demais fatores, a neoplasia maligna indiferenciada apresenta um maior risco de morte. As magnitudes dos coeficientes de incid?ncia encontradas n?o s?o consideradas elevadas, enquanto que de forma contr?ria est?o as magnitudes dos coeficientes de mortalidade