Le terme de CLIP-170 désigne la protéine de lien cytoplasmique de 170 KDa, isolée par Rickard et Kreis (1990). In vitro, elle constitue un lien statique entre les endosomes et les microtubules (MTs). En chacun des points où la CLIP-170 est localisée, elle se co-distribue avec le complexe moteur dynéine/dynactine (D/D). Les auteurs ont initialement établi un modèle de fonctionnement de concert de ces trois protagonistes : la CLIP-170 établirait le lien initial entre le cargo et le MT. Le complexe D/D serait recruté sur le cargo. Une fois le moteur associé au MT, le lien statique serait levé, rendant possible le mouvement. Ce modèle offrait une explication aux données d'immunolocalisation. Toutefois, le fonctionnement de concert de la CLIP-170 et du moteur moléculaire nécessitait que l'on puisse trouver une interaction entre les protagonistes. Les travaux présentés dans cette thèse apportent pour la première fois la preuve d'une interaction indirecte entre le complexe D/D et la CLIP-170. LIS1 est une protéine codée par le gène causal du syndrome de Miller-Dieker, une forme de lissencéphalie de type I. Elle sert d'adaptateur entre le domaine carboxy-terminal de la CLIP-170 et le complexe moteur dont elle régule l'activité. Nos résultats établissent que le domaine d'interaction de la CLIP-170 avec LIS1 est requis pour l'adressage de la première aux kinétochores prémétaphasiques. Il est nécessaire à l'adressage de LIS1 (et du complexe moteur) aux bouts (+) des MTs interphasiques. Nous présentons deux nouvelles approches permettant d'interférer avec le fonctionnement de la CLIP-170 tant en interphase qu'en mitose : la microinjection d'anticorps dirigés contre les domaines extrêmes de la protéine, ainsi que l'utilisation de siRNA dirigés contre l'ARNm la codant. Combinées aux techniques de suivi de protéines fluorescentes par vidéomicroscopie 3D développées au laboratoire, elles permettront d'interférer avec le fonctionnement de la CLIP-170 et de définir ainsi son rôle.

Submitted by Erika Demachki (erikademachki@gmail.com) on 2018-06-29T17:45:52Z No. of bitstreams: 2 Dissertação - Alysson Plínio Estevo - 2018.pdf: 6408496 bytes, checksum: cc40829e6309168894724e8b4bce925b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2018-07-03T14:54:52Z (GMT) No. of bitstreams: 2 Dissertação - Alysson Plínio Estevo - 2018.pdf: 6408496 bytes, checksum: cc40829e6309168894724e8b4bce925b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2018-07-03T14:54:52Z (GMT). No. of bitstreams: 2 Dissertação - Alysson Plínio Estevo - 2018.pdf: 6408496 bytes, checksum: cc40829e6309168894724e8b4bce925b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2018-05-02 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / This exploratory research is based on theoretical investigation as well as on the artistic practice of black metal, one of the many branches of heavy metal. It aims to analyze its aesthetics and poetics as well as study its video clips language and their new emerging audiovisual narratives, focusing on the “interactive video clip”. The essay’s proposal comes from the researcher’s own experience with black metal in the Brazilian scenario, which was possible because of his authorial band “Luxúria de Lilith”. The research involves the new possibilities of fruition through the creation of a visual interactive narrative based on one of the songs from “Luxúria de Lilith”’s black metal opera, Gehennom. It is part of a master’s degree final paper, presenting a fictional and transmediatic universe that explores the poetics of the interactive video clip. / Essa pesquisa exploratória tem como base a investigação teórica e a prática artística, objetivando pesquisar a estética e a poética do subgênero musical black metal, uma das vertentes do heavy metal, assim como a linguagem do videoclipe e das novas narrativas audiovisuais emergentes, focando no chamado “videoclipe interativo”. A proposta parte da experiência do pesquisador no âmbito da cena brasileira do black metal durante 20 anos, por meio de sua banda autoral “Luxúria de Lillith”, e do estudo de suas motivações conceituais, as mesmas que caracterizam o referido gênero musical. Também envolve a pesquisa sobre o videoclipe e as suas novas possibilidades fruitivas através da criação de uma narrativa visual interativa tendo como base uma das músicas da ópera black metal Gehennom composta pela banda “Luxúria de Lillith” como parte dessa dissertação de mestrado, apresentando seu universo ficcional transmidiático criado para explorar a poética do videoclipe interativo.

Master of Science / Department of Architectural Engineering / Kimberly W. Kramer / In structural steel connection design, simple shear connections are one of the most common connection types utilized. The industry, especially from the side of the engineer, tends to lean toward using Double Clip Angle Connections as the default standard for simple shear connections. A double clip angle connection is a connection consisting of two angles transferring the shear forces from one member to the next either through bolts or welds. The design of Double Clip Angle Connections is efficient and the connections themselves are easy to fabricate. However, benefits to utilizing other types of shear connections exist. Many of these benefits are seen in the fabrication shop or during erection and construction. This is especially true of single shear plate or shear tab connections when applied to open structure design. Shear tab connections consist of a single plate that transfers the shear forces from one member to the next with bolts or with welds. The design of shear tab connections can be a more involved process than the design of double clip angles. Sometimes the shear plate or shear tab has to be longer than is typical. This is called an extended shear plate connection. These extended shear plates can bring other variables into the design that typically don’t occur with Double Clip Angle Connections such as bending of the plate or the need for multiple bolt columns. However, with proper planning and detailing, the benefits and savings experienced in the fabrication or construction phase may outweigh what can be seen as a more laborious design task. The purpose of this report is to identify the possible benefits achieved in using each of these connections, highlight the differences in the design approach for each, and use a study model to compare the outcome of using one connection over another in the design of a typical open structure. Double clip angles are typically the most efficient approach when speed of design and simplicity of fabrication are the desired outcomes. However, shear plate or shear tab connections have the potential to provide safer erection alternatives and materials savings if used in appropriate ways and with the right applications.

South Africa is considered the primary producer and most reliable source of high quality clip mohair in the world. The application of molecular technologies to improve mohair quality is relatively new to this industry. The aim of the study was to use a molecular approach to genetically improve South African Angora goats, with emphasis on mohair production. A reference population of Angora goats was firstly established consisting of twelve sire families with half-sib offspring (1067 individuals in total). The genetic variation of this population was evaluated using microsatellite markers and the average gene diversity was found to be above 60%. Ninety four microsatellite markers were then genotyped on the reference population, spanning 23 chromosomes (total length 1352cM) with an average marker interval of 23.0cM. This information was used to improve previously published goat linkage maps. Unmapped microsatellite markers were incorporated and previously published inter-chromosomal rearrangements between the goat and sheep genetic maps were confirmed or rejected. Nine new markers were mapped to the goat genome, and six chromosomes showed rearrangement when compared to the previous goat map. Four previously reported intra-chromosomal rearrangements were shown to be either population specific or mapping errors. Variance components and genetic parameters of mohair traits (FW, FD, CVFD, SDFD, CF, SF and SDA) were estimated; including the fibre diameter profile measured using OFDA technology that has not yet been included in genetic evaluations. Heritability estimates ranged between 0.14 (SDA) and 0.63 (CF). OFDA-measured traits should be considered for inclusion into the national breeding strategy. The reference population was lastly analysed to identify QTL associated with fleece traits. Eighteen putative QTL were identified for seven mohair traits on 13 chromosomes. Three putative QTL were detected for FW on CHI 2, 5 and 24 corresponding with KRT and KAP gene locations. Two QTL associated with mohair FD (on CHI 4 and 24) were detected. QTL contributions to variance ranged between 7.44% (CF) and 19.69% (SDA). The results of this study should form part of an integrated approach where both quantitative and molecular tools are applied for genetic improvement of South African Angora goats. / Thesis (PhD)--University of Pretoria, 2011. / Animal and Wildlife Sciences / PhD / Unrestricted

This thesis presents experiments and numerical analysis of the cold-formed steel clip angle in three different limit states which are shear, compression, and combination of the screw connection. A previous cold-formed steel clip angle test program (which is Phase 1) developed design methods for clip angle. Therefore, the object of this thesis is to further investigate the behavior and design methods of loading-bearing cold-formed steel clip angles under different screw pattern. For each limit state, a test program was conducted to investigate the behavior, strength, and deflection of the clip angle. The test result were compared with previous CFS clip angle design method. Amending existing CFS clip angle method were developed by each of the four limit states studied in this project.

The thesis is based on analysis of current situation in the field of multimedial presentations of universities. In addition, a multimedial presentation in the form of videoclips was proposed, along with its analysis and evaluation.

No description available.

I Abstract (in English): Diploma thesis Visual lifelogging: automatic memories and picturing ordinary deals with the topic of visual lifelogging in the context of current visual culture. It describes visual lifelogging in the context of photography and as an amplification of human abilities. Diploma thesis deals with history and lifelogging as a whole and it describes its forms, describes the tools of lifelogging and it deals also with topics of surveillance and its contrary, sousveillance. It explores visual lifelogging mostly from the photographic point of view and it deals with the use of different technologies to augment human memory or other human abilities.

RBMS3 belongs to the family of c-myc gene single-strand binding proteins (MSSPs) that play important roles in transcriptional regulation. Here, we show that RBMS3 functions as a tumor promoter in triple-negative breast cancer (TNBC), a highly aggressive BC subtype. Analysis of RBMS3 expression shows that RBMS3 is upregulated at both mRNA and protein levels in TNBC cells. Functionally, overexpression of RBMS3 increases cell migration, invasion and cancer stem cell (CSC) behaviors. Moreover, RBMS3 induces expression of epithelial-mesenchymal transition (EMT) and CSC markers. Conversely, loss of RBMS3 in TNBC BT549 cells inhibits cell proliferation, migration and mesenchymal phenotype. Correlation analysis shows RBMS3 is associated with TGF-β signaling. Mechanistically, RBMS3 interacts with Smad2, Smad3 and Smad4 mRNA and regulates the stability of these transcripts. Importantly, RBMS3 prevents TGF-β-induced cytostasis and apoptosis in premalignant cancer cells. Moreover, RBMS3 inversely correlates with expression of ESRPs, epithelial-specific splicing regulatory proteins that regulate morphogenesis-associated alternative splicing events. ESRPs appear to suppress EMT through distinct mechanisms: ESRP1 restricted cell migration, whereas ESRP2 prevented cell growth. RBMS3 significantly facilitates the EMT process when ESRPs are lost. Collectively, the studies within this dissertation identify RBMS3 as a positive regulator of EMT and breast cancer progression by regulating the TGF-β signaling pathway.

From creation to its degradation, the RNA molecule is the action field of many binding proteins with different roles in regulation and RNA metabolism. Since these proteins are involved in a large number of processes, a variety of diseases are related to abnormalities occurring within the binding mechanisms. One of the experimental methods for detecting the binding sites of these proteins is PAR-CLIP built on the next generation sequencing technology. Due to its size and intrinsic noise, PAR-CLIP data analysis requires appropriate pre-processing and thorough statistical analysis. The present work has two main goals. First, to develop a modular pipeline for preprocessing PAR-CLIP data and extracting necessary signals for further analysis. Second, to devise a novel statistical model in order to carry out inference about presence of protein binding sites based on the signals extracted in the pre-processing step.