Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-12-12T19:35:46Z No. of bitstreams: 1 RodrigoXavierSoares_TESE.pdf: 3740466 bytes, checksum: ff614cd85293ff3942f03a08100795a3 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-12-14T18:36:47Z (GMT) No. of bitstreams: 1 RodrigoXavierSoares_TESE.pdf: 3740466 bytes, checksum: ff614cd85293ff3942f03a08100795a3 (MD5) / Made available in DSpace on 2017-12-14T18:36:47Z (GMT). No. of bitstreams: 1 RodrigoXavierSoares_TESE.pdf: 3740466 bytes, checksum: ff614cd85293ff3942f03a08100795a3 (MD5) Previous issue date: 2017-05-12 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Grandes peixes pel?gicos representam um dos principais e mais lucrativos alvos da pesca industrial no Brasil. O Rio Grande do Norte pela posi??o geogr?fica privilegiada constitui hoje uma das regi?es em que a pesca oce?nica comercial ? mais produtiva com tendencia a amplia??o a partir de novas pol?ticas de incentivo governamental. Diferente de outras regi?es produtoras mundiais que v?m pesquisando as principais esp?cies exploradas, no Brasil estudos que objetivem a delimita??o de popula??es e dados gen?ticos para a conserva??o das esp?cies marinhas s?o escassos. Dentro dos grupos pel?gicos predomina menor diversidade em rela??o ? abund?ncia, isto ? caracter?stico em algumas das mais importantes fam?lias de peixes desta regi?o oce?nica, como Sphyraenidae (barracudas), Carangidae (xareus e pampos), Coryphaenidae (dourados) e Istiophoridae (marlins). A obten??o de dados citogen?ticos destas esp?cies se revestem de dificuldades log?sticas, tanto pelo tamanho dos exemplares, quanto pela forma de captura e habitat que ocupam. As primeiras informa??es citogen?ticas para algumas esp?cies destas fam?lias t?m apenas recentemente sido obtidas. Aqui ? realizado um amplo levantamento citogen?ticas de 9 esp?cies de peixes pel?gicos Atl?nticos, das fam?lias Sphyraenidae, Carangidae, Corypahenidae, Istiophoridae e Megalopidae e 1 Carangidae do Indo-Pac?fico. Os resultados revelaram diverg?ncias num?ricas e na macroestrutura cariot?pica, em contraste ao padr?o considerado basal para Teleostei, al?m da ocorr?ncia de um sistema de cromossomos sexuais m?ltiplos do tipo X1X1X2X2/X1X2Y em Coryphaenidae. A partir de t?cnicas como Ag-RONs, MM/DAPI foi poss?vel identificar a utilidade de s?tios ribossomais simples e sua localiza??o como eficientes marcadores citotaxon?micos para todas as esp?cies. T?cnicas citogen?ticas mais resolutivas de mapeamento de sequ?ncias multig?nicas (FISH - Fluorescence in situ Hybridization) possibilitaram pela primeira vez para algumas dessas fam?lias inferir sobre os processos carioevolutivos vigentes e aspectos evolutivos das esp?cies estudadas. A partir dos resultados obtidos ampliou-se o conhecimento sobre estes importantes recursos marinhos, subsidiando pol?ticas futuras de manejo dos estoques, bem como esteio futuro para o desenvolvimento tecnol?gico da aquacultura marinha. / The big pelagic fish represents one of the most lucrative subjects of the industrial fisheries in Brazil. The Rio Grande do Norte by the privileged geographic position constitutes nowadays one of the regions in which the commercial oceanic fishing is more productive and tends to expand with new policies of governmental incentive.Different from other World?s producer regions that are researching the main exploited species, in Brazil the studies that objectify the population delimitation and to list data for the conservation of the species are scarce. Among the pelagic groups is common to observe a predominance of a minor diversity in relation to the abundance, this is a characteristic in two of the most important families of this oceanic region, Sphyraenidae (barracudas), Carangidae (jacks and pompanos) and Coryphaenidae (dolphinfish) and Istiophoridae (marlins). Data about these species are surrounded of logistics difficulties for its size, capture way and habitat which occupies. The first cytogenetical informations for some species of this family were obtained only recently. Here is presented a cytogenetical study with 9 pelagic fish species from the Atlantic, of the families Sphyraenidae, Carangidae, Corypahenidae, Istiophoridae and Megalopidae and 1 Indo-Pacific Carangidae. The results revealed numerical divergences and in the karyotipical macrostructure contrasting with the pattern considered basal for the Teleosts, besides the existence of masculine heterogamety, through a system of multiple sexual chromosomes of the kind X1X1X2X2/X1X2Y. Using techniques such as AgNORs, MM/DAPI it was possible to identify single ribosomal sites and showed cytotaxonomic markers for all. It was also applied more resolutive cytogenetic techniques based on multigenic sequences mapping through FISH (Fluorescence in situ Hybridization) enabled for the first time for some of these families to infer about the active karyoevolutive processes and evolutive aspects of the investigated species. Through the developed techniques it will be also possible to expand the knowledge about this important marine resource,subsidizing future policies of the management of stocks, as well as future support for the technological development of marine aquaculture.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-06-13T19:59:23Z No. of bitstreams: 1 AndreLeandroSilva_TESE.pdf: 10340347 bytes, checksum: 7772f16f42cbf49c9b19bfcc055d96c2 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-06-19T11:52:35Z (GMT) No. of bitstreams: 1 AndreLeandroSilva_TESE.pdf: 10340347 bytes, checksum: 7772f16f42cbf49c9b19bfcc055d96c2 (MD5) / Made available in DSpace on 2017-06-19T11:52:35Z (GMT). No. of bitstreams: 1 AndreLeandroSilva_TESE.pdf: 10340347 bytes, checksum: 7772f16f42cbf49c9b19bfcc055d96c2 (MD5) Previous issue date: 2017-04-20 / A anfotericina B (AmB) ? um f?rmaco de caracter?sticas f?sico-qu?micas bastante peculiares: de car?ter anfif?lico e anfot?rico. Essas caracter?sticas tornam dif?cil sua veicula??o em sistemas terap?uticos. Atualmente, a AmB ? veiculada por via intravenosa nas formas de micelas, lipossomas e complexos lip?dicos. A literatura demonstra que existe uma ?ntima rela??o entre a forma como a AmB est? complexada ao sistema carreador e sua resposta biol?gica. Entretanto, h? uma defici?ncia nos dados de caracteriza??o fisico-qu?mica dos produtos dispon?veis contendo AmB. Portanto, o objetivo deste trabalho foi realizar a caracteriza??o f?sico-qu?mica de sistemas carreadores de AmB visando uma predi??o de sua resposta biol?gica. Os sistemas micelares de AmB foram os primeiros produtos dispon?veis ? pr?tica cl?nica, de forma que sua patente expirou h? alguns anos. Neste trabalho, o sistema original e dois de seus similares foram caracterizados e o aumento da estabilidade destes sistemas ap?s aquecimento, pela forma??o dos super-agregados de AmB foi estudado. O sistema liposomal AmBisome? tamb?m foi caracterizado e, pela primeira vez, foi estudada a possibilidade de super-agrega??o, a exemplo dos sistemas micelares, a partir de lipossomas. A incorpora??o de AmB em sistemas nano e microemulsionados foi apresentada e as caracter?sticas f?sico-qu?micas destes sistemas foram estudados, com demonstra??o de suas aplica??es no tratamento ocular de infec??es f?ngicas e tamb?m para o tratamento de leishmaniose visceral. As principais t?cnicas de caracteriza??o aplicadas foram espectroscopia UV-Vis, dicro?smo circular e espalhamento din?mico de luz. A t?cnica de calorimetria de titula??o isot?rmica (ITC) foi utilizada numa tentativa de medir a energia de forma??o dos superagregados. Adicionalmente, um derivado sol?vel de AmB foi desenvolvido e caracterizado por espectroscopia de massa at?mica, infra-vermelho, UV-Vis e dicro?smo circular, bem como incorporado em sistema microemulsionado como estrat?gia de veicula??o deste derivado sol?vel. Os resultados revelam que os sistemas contendo AmB apresentam diferentes formas de agrega??o molecular dependendo do carreador, da forma de incorpora??o do f?rmaco e do diluente empregado para redispersar o sistema. Segundo a literatura, o estado de agrega??o est? intimamente ligado ? efic?cia e ? toxicidade da mol?cula. Nos sistemas nanoemulsionados a AmB apresenta-se na forma agregada e multi-agregada. Na microemuls?o, est? incorporada na forma monom?rica. Os sistemas micelares aquecidos d?o lugar ? forma??o de super-agregados de AmB enquanto os sistemas lipossomais s?o incapazes de se modificar em super-agregados. O derivado sol?vel de AmB apresentou caracter?sticas que diferem da AmB original. Contudo, quando incorporado na microemuls?o, o estado de agrega??o ? similar ao da mol?cula original tanto nas an?lises de UV-Vis quanto de dicro?smo circular. Pode-se concluir que a forma de agrega??o de AmB varia n?o somente de acordo com o tipo de carreador, mas tamb?m com sua concentra??o no meio e com o tipo de incorpora??o, ainda que num mesmo tipo de carreador. Finalmente, o derivado sol?vel abre a possibilidade de veicula??o do f?rmaco em carreadores de car?ter aquoso para o tratamento de diversas enfermidades e por v?rias vias de administra??o. / The amphotericin B (AmB) is a drug of peculiar physicochemical features: being amphiphilic and amphoteric. These characteristics turn difficult the drug load into therapeutic systems. AmB is currently available in the market as micelles, liposomes and lipid complex for injection. The literature show that there is an intimate correlation between the AmB bound to the carrier and its biological response. However, there is a deficiency concerning the physicochemical characterization of the available AmB-containing products. Therefore, the aim of this work was to characterize AmB-containing carriers seeking a prediction to its biological response. The AmB-containing micellar system was the first product available for clinical use. The patent of this product has already expired some years ago. In this work we have characterized the original system and two other similar micellar products. In addition, we studied the stability increase of heated systems, by the formation of AmB ?super-aggregates?. AmBisome?, an AmB-containing liposomal system, was also characterized and, for the first time, tested for the possibility of super-aggregates formation. The AmB incorporation into nano and microemulsion systems was presented and the physicochemical characteristics evaluated, focusing mainly on applications for the treatment of fungal ocular diseases and also for visceral leishmaniasis. The main techniques used for characterization were electronic spectroscopy, circular dichroism and dynamic light scattering. The isothermal titration calorimetry (ITC) was used as an attempt to measuring the super-aggregates energy formation. Besides, an AmB soluble derivative was developed and characterized by atomic mass spectroscopy, infra-red, UV-Vis and circular dichroism. Then, this AmB-derivative was loaded into a microemulsion as a vehiculation strategy. The overall results show that the AmB-containing systems presented different molecular aggregation states that depends on the carrier, the way the drug is incorporated and also on the diluent. According to the literature, the aggregation state is associated with both, drug efficiency and toxicity. In nanoemulsion systems, the drug is found aggregated and multi-aggregated. In microemulsions, AmB is loaded as monomers. The heated micellar systems form AmB super-aggregates while the liposomal system is unable to form such molecular structure. Moreover, the AmB soluble derivative presented distinct features when compared to the original molecule. However, once incorporated into the microemulsion, the aggregation state is similar to that of the original AmB molecule, as supported by UV-Vis and circular dichroism results. It can be concluded that the AmB aggregation state varies according to the kind of carrier, the drug concentration and also the way of drug incorporation, even into one same carrier. Finally, the soluble derivative opens the possibility for drug carrying into aqueous vehicles for the treatment of many diseases by different administration routes. / 2018-04-27

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-06-13T19:59:23Z No. of bitstreams: 1 HenriqueJorgeMaiaCosta_TESE.pdf: 2983042 bytes, checksum: e69d54482caf7ae9786e28e71023539e (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-06-19T12:14:15Z (GMT) No. of bitstreams: 1 HenriqueJorgeMaiaCosta_TESE.pdf: 2983042 bytes, checksum: e69d54482caf7ae9786e28e71023539e (MD5) / Made available in DSpace on 2017-06-19T12:14:15Z (GMT). No. of bitstreams: 1 HenriqueJorgeMaiaCosta_TESE.pdf: 2983042 bytes, checksum: e69d54482caf7ae9786e28e71023539e (MD5) Previous issue date: 2016-08-29 / O jejum pr?-operat?rio foi institu?do h? muitos anos, no in?cio das pr?ticas anest?sicas, para garantir o esvaziamento do conte?do g?strico, evitar bronco-aspira??o, v?mitos, regurgita??o, administra??o de v?rias drogas para reduzir a acidez g?strica e volume. Protocolos de jejum pr?-operat?rio sugerem per?odos menores de jejum, principalmente para l?quidos, permitindo mais conforto aos pacientes, e menor risco de hipoglicemia e desidrata??o, sem aumentar a incid?ncia de aspira??o pulmonar perioperat?ria. O objetivo do estudo foi avaliar se o bioproduto ? base de ?gua de coco e maltodextrina (ACP Surgery) supria as necessidades dos protocolos cl?nicos de jejum pr?-operat?rio de curta dura??o do projeto ACERTO (Acelera??o da Recupera??o Total P?s-operat?ria) com boa toler?ncia e aceita??o. Foi realizado um estudo prospectivo, aleat?rio, controlado e duplo cego em seres humanos, em um grupo de 49 pacientes na Santa Casa de Miseric?rdia de Fortaleza, para comprovar a efici?ncia e efic?cia do ACP Surgery para atingir as metas e efeitos planejados nos protocolos de jejum pr?-operat?rio de curta dura??o. Os grupos experimentais foram: G1 [grupo controle; Nutri Dextrin (Nutrimed, Fortaleza, Cear?); n = 20) e G2 [interven??o; ACP Surgery (ACP Biotecnologia, Fortaleza, Cear?; n = 29]. No intervalo entre 10 e 20 horas da realiza??o da cirurgia, amostra de sangue era colhida de cada paciente e encaminhada para o Laborat?rio de An?lises Cl?nicas do hospital para mensura??o dos n?veis s?ricos de leuc?citos totais, glicose, s?dio, pot?ssio e cloro. Os dados foram submetidos ao teste t (? = 95%, p > 0,05 e ?rea de rejei??o t > 1,96) e expressos em m?dia e desvio padr?o. Os grupos G1 (Nutri Dextrin) e G2 (ACP Surgery) apresentaram os seguintes resultados quanto ?s an?lises sangu?neas: leuc?citos totais G1 = 11.322,64 + 5.033,94, e G2 = 11.074,14 + 4.480,35; s?dio G1 = 140,8 + 4,14 mEq/l, e G2 = 139,3 + 4,38 mEq/l; pot?ssio G1 = 4,16 + 0,65 mEq/l, e G2 = 4,18 + 0,47 mEq/l; cloro G1 = 107,4 + 4,69 mEq/l, e G2 = 107,1 + 6,88 mEq/l; e glicose G1 =130,3 + 39,96 mg/dl, e G2 = 123,9 + 36,47 mg/dl. Em todas as vari?veis estudadas o p > 0,05, portanto, pode-se considerar que os produtos Nutri Dextrin e ACP Surgery s?o equivalentes. A aceita??o do ACP Surgery foi de 97%, sem relatos de pigarro ou n?useas (toler?ncia de 100%), sem perda de palatabilidade. Conclui-se que o bioproduto produzido supriu as necessidades e especifica??es dos protocolos de jejum pr?-operat?rio de curta dura??o apresentando aceita??o e toler?ncia compat?vel com o produto comercial ao mesmo tempo em que reduziu os custos e facilitou a execu??o do protocolo. / The preoperative fasting was instituted many years ago, at the beginning of the anesthetic practice, to ensure the emptying of the stomach contents, avoid bronchial aspiration, vomiting, regurgitation, administration of various drugs to reduce gastric acidity and volume. Preoperative fasting protocols suggest shorter fasting periods especially for liquids, providing more comfort to the patient, and less risk of hypoglycemia and dehydration, without increasing the incidence of perioperative pulmonary aspiration. The aim of the study was to assess whether the bioproduct based on coconut water and maltodextrin (ACP Surgery) supplied the needs of clinical trials of ERAS project (Enhanced Recovery After Surgery) with good tolerance and acceptance. It was conducted a prospective, randomized, controlled, double-blind study in humans with a group of 49 patients at the Santa Casa de Misericordia de Fortaleza, to prove the efficiency and effectiveness of ACP Surgery to achieve the goals and planned effects on ERAS project. The experimental groups were: G1 [control group; Nutri Dextrin (Nutrimed, Fortaleza, Cear?); n = 20) and G2 [intervention; ACP Surgery (ACP Biotecnologia, Fortaleza, Cear?;. N = 29]. In the interval between 10 and 20 hours prior to the surgery, blood sample was taken from each patient and sent to the Hospital's Laboratory of Clinics Analysis for measurement of serum levels of total leukocytes, glucose, sodium, potassium and chlorine. The data were submitted to t test (? = 95%, p> 0.05 and t rejection area > 1.96) and expressed as mean and standard deviation. The G1 (Nutri Dextrin) and G2 (ACP Surgery) showed the following results regarding blood tests: total leukocyte G1 = 11,322.64 + 5,033.94, and G2 = 11,074.14 + 4,480.35; sodium G1 = 140.8 + 4.14 mEq/L, and G2 = 139.3 + 4.38 mEq/L; potassium G1 = 4.16 + 0.65 mEq/L, and G2 = 4.18 + 0.47 mEq/L; chloro G1 = 107.4 + 4.69 mEq/L, and G2 = 107.1 + 6.88 mEq/L; and glucose G1 = 130.3 + 39.96 mg/dL, and G2 = 123.9 + 36.47 mg/dL. In all variables p> 0.05, so it can be considered that Nutri Dextrin and ACP Surgery products are equivalent. The acceptance of the ACP Surgery was 97%, with no reports of cough or nausea (100% tolerance), without loss of palatability. It follows that the produced bioproduct met the needs and specifications of preoperative fasting protocols of short duration, showing acceptance and tolerance compatible with the commercial product at the same time reducing costs and facilitating protocol implement.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-12-12T19:35:46Z No. of bitstreams: 1 MarcelinoGevilbergueViana_TESE.pdf: 5841614 bytes, checksum: a1942694c0220572b2895b7fa57c26cc (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-12-14T18:31:11Z (GMT) No. of bitstreams: 1 MarcelinoGevilbergueViana_TESE.pdf: 5841614 bytes, checksum: a1942694c0220572b2895b7fa57c26cc (MD5) / Made available in DSpace on 2017-12-14T18:31:11Z (GMT). No. of bitstreams: 1 MarcelinoGevilbergueViana_TESE.pdf: 5841614 bytes, checksum: a1942694c0220572b2895b7fa57c26cc (MD5) Previous issue date: 2017-08-31 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / A corros?o microbiologicamente induzida (CMI) atinge todos os setores de produ??o da ind?stria de petr?leo e g?s e demanda expressivos gastos e investimentos para preven??o e controle. O objetivo dessa tese foi avaliar as atividades biocida e antibiofouling de metab?litos de microrganismos endof?ticos de Lippia gracilis Schauer como tamb?m do ?leo essencial (puro e em sistema microemulsionado) dessa esp?cie sobre biofilmes microbianos em a?o carbono 1020 AISI. Os experimentos utilizaram cupons de a?o carbono 1020 AISI dispostos em Erlenmyers e em sistema din?mico para a obten??o dos biofilmes. A avalia??o biocida dos metab?litos deu-se pela quantifica??o microbiana pelas t?cnicas de absorb?ncia, do n?mero mais prov?vel (NMP), antes e ap?s os tratamentos. Os tratamentos consistiram em: 0, 100, 150 e 200 ?g/L de cada metab?lito sobre biofilmes nos tempos de contato zero, duas, tr?s e quatro semanas, com tr?s repeti??es por tratamentos. A a??o antibiofouling foi realizada pelo contato dos metab?litos na maior concentra??o (200 ?g/L) com os biocupons antes da obten??o dos biofilmes. A a??o antimicrobiana do ?leo essencial de L. gracilis Schauer na forma pura, e em sistema microemulsionado, foi avaliada pela quantifica??o de bact?rias redutoras de sulfato (BRS), oxidantes de ferro (BOF), fungos, bact?rias aer?bias e anaer?bias totais, pelas t?cnicas de NMP, plaqueamento pour plate, espectrofotometria de massa (EM) e an?lise de micrografias realizadas antes e ap?s os tratamentos. A atividade anticorrosiva foi avaliada por imped?ncia e polariza??o eletroqu?micas, testes gravim?tricos (perda de massa e quantifica??o de pites) bem como pela obten??o de micrografias da superf?cie do a?o carbono. Amostras de L. gracilis Schauer foram coletadas no munic?pio de Mossor? - RN e foram isolados fungos e bact?rias das ra?zes e folhas. Uma nova esp?cie de fungo foi descoberta e identificada como Achaetomium lippae. Foram obtidos tr?s metab?litos: dois de fungos e um de bact?ria. Os resultados mostraram que houve diferen?a da atividade biocida e antibiofouling entre os metab?litos de endof?ticos de L. gracilis Schauer avaliados, sendo LGS-Rb1 o mais eficiente na maioria tipos microbianos testados, evitando a forma??o de biofouling e reduzindo a taxa de crescimento microbiano na maior concentra??o avaliada. Os ensaios eletroqu?micos e gravim?tricos mostraram que para todos os metab?litos houve varia??o na atividade anticorrosiva. O ?leo essencial de L. gracilis Schauer avaliado in natura inibiu significativamente o crescimento in vitro de C. bifermentans e de fungos em biofilme, reduzindo sua taxa de crescimento a zero a partir de 20 ?g.L-1. Os ensaios eletroqu?micos e gravim?tricos mostraram que a concentra??o de 60 ?g.L-1 do ?leo essencial foi eficiente na redu??o da corros?o. Nos ensaios usando o sistema microemulsionado o ?leo essencial inibiu eficientemente o crescimento de BRS, BOF e fungos ap?s 16 dias de contato e teve atividade antibiofouling ap?s 96 horas de contato com os biocupons de a?o carbono AISI 1020. Dessa forma os metab?litos de endof?ticos de L. gracilis Schauer bem como seu ?leo essencial mostram-se como novas alternativas de biocidas naturais eficientes no controle da corros?o microbiologicamente induzida. / The corrosion microbiologically induced (CMI) reaches all the sectors of production of the industry in Petroleum oil and Gas and demands expressive expensive and investments for prevention and control. The goal of this thesis was to evaluate the biocide and antibiofouling activities of secondary metabolites obtained from endophytic microorganisms of Lippia gracilis Schauer as well of the essential oil (pure and in microemulsified system) from this species on microbial biofilms in 1020 AISI carbono steel. The experiments for biocide activity used 1020 AISI carbon steel plate arranged in Erlenmyers and dynamic system with forced circulation for obtaining of the total biofilms from water produced by oil. The assessment for biocide of the metabolites from endophytic of L. gracilis Schauer resulted of the quantification of these species of bacteria in the quantification techniques by absorbance in more probable numbers (MPN), before and after the treatments, had been identified by gas chromatography coupled the spectrophotometry in mass (CG-MS) and magnetic resonance imaging (MRI) just those who demonstrated activities. The treatments consisted in evaluating the concentrations 0, 100, 150 and 200 ?g/L in each metabolites on biofilms in times contact to zero, two, three and four weeks, with three repetition by treatments. The antibiofouling action was made in the contact of the metabolites in the highest concentration (200 ?g/L) with the bioplates before being inserted into the dynamic system. The antimicrobial action of the essential oil L. gracilis Schauer in the pure form and in a microemulsified system was evaluated by the quantification bacteria reducer of sulphate (BRS), oxidant of iron (BOI), fungi, aerobic bacteria and total anaerobes by the techniques of dilution, platingpourplate, spectrophotometry in mass (MS) and micrographs analysis made before and after the treatments. The anticorrosive activity of all bioproducts was evaluated by electrochemical impedance and polarization, gravimetric examinations of mass loss and quantification of pites, as well in the obtaining of micrographs of the carbon steel`s surface. Samples of L. gracilis Schauer were collected in the city of Mossor? - RN and were isolated fungi and bacteria from the roots and leaves. A new type of fungus was discovered and identified as Achaetomium lippae. Three metabolites were obtained: two fungi (LGS-Lf1 and LGS-Lf2, being "LGS" for L. gracilis Schauer, "L": leaf, ?f?: fungus and "R" for root) and one of bacteria (LGS-Rb1, being "LGS" for L. gracilis Schauer, "R": root and "b": bacterium). The results showed that there was a difference in the biocide activity and antibiofouling between the metabolites from endophytic of L. gracilis Schauer assessed, being just the LGS-Lf1 being efficient over all the microorganisms tested, avoiding the formation of biofouling on the surface of the material and reducing the rate of microbial growth at zero after 96 hours of contact at the highest concentration evaluated. The electrochemical and gravimetric tests displayed that for all the metabolites occured change in the anticorrosive activity. The L. gracilis Schauer essential oil evaluated in the pure form inhibited significally the growth in vito of C. bifermentans and of fungi in biofilm, reducing their growth rate to zero as from 20 ?g.L-1. The electrochemical and gravimetric tests showed that the concentration of 60 ?g.L-1 of the essential oil was efficient in the reduction of corrosion. In the tests, using the microemulsified system, the essential oil inhibited the growth of BRS, BOF and fungi 16 days later in contact and had antibiofouling activity after 96 hours in contact with the AISI 1020 bioplates of carbon steel. Therefore, the metabolites from endophytic of L. gracilis Schauer as well its essential oil are shown as new alternatives of natural biocides efficient in the control of the corrosion microbiologically induced.