El objetivo primordial del presente proyecto es el desarrollo y elaboración de un producto saludable como respuesta a una necesidad identificada en todas estas personas que buscan cuidar su apariencia física y su salud. Nuestra propuesta es un alimento elaborado a base de quinua con un alto valor nutricional que contribuye al cuidado de la salud y estado físico de las personas que buscan mantener una buena apariencia física y velar por su salud consumiendo productos saludables. Nos enfocamos en los NSE "A" y "B" de ambos sexos, entre las edades de 18 a 55 años, principalmente estudiantes y personas trabajadoras con una estabilidad laboral, lo cual nos permitió detectar una demanda insatisfecha debido a la falta de alimentos saludables y nutritivos al alcance de las mismas. La tendencia al cuidado personal de la apariencia física y de la salud van de la mano y crecen día a día en la actualidad. Es por ello que se crea “Healthy Food”, el cual significa "comida sana", para poder cubrir esta necesidad detectada y atender este mercado potencial no explotado. / The main objective of this project is the development and preparation of a healthy product as a response to a need identified in all these people who seek to take care of their physical appearance and health. Our proposal is a food based on quinoa with a high nutritional value that contributes to the health care and physical condition of people who seek to maintain a good physical appearance and ensure their health by consuming healthy products. We focus on the NSE "A" and "B" of both sexes, between the ages of 18 to 55, mainly students and working people with job stability, which allowed us to detect an unmet demand due to the lack of healthy foods and nutritious within reach of them. The tendency to take personal care of physical appearance and health goes hand in hand and is growing day by day nowadays. That is why "Healthy Food" is created, which means "healthy food", to be able to cover this detected need and attend to this untapped potential market. / Trabajo de investigación

Submitted by Gabriela Lopes (gmachadolopesufpel@gmail.com) on 2018-06-25T17:36:20Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) dissertação vergara final - Cópia.pdf: 685849 bytes, checksum: 100fb54a99f7c73ef885ae365bb5efa1 (MD5) / Approved for entry into archive by Aline Batista (alinehb.ufpel@gmail.com) on 2018-07-03T18:15:04Z (GMT) No. of bitstreams: 2 dissertação vergara final - Cópia.pdf: 685849 bytes, checksum: 100fb54a99f7c73ef885ae365bb5efa1 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Aline Batista (alinehb.ufpel@gmail.com) on 2018-07-03T18:37:22Z (GMT) No. of bitstreams: 2 dissertação vergara final - Cópia.pdf: 685849 bytes, checksum: 100fb54a99f7c73ef885ae365bb5efa1 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2018-07-03T18:37:36Z (GMT). No. of bitstreams: 2 dissertação vergara final - Cópia.pdf: 685849 bytes, checksum: 100fb54a99f7c73ef885ae365bb5efa1 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2016-12-14 / Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq / A quinoa é uma planta da família Chenopodiacea, domesticada pelos povos andinos há milhares de anos. É uma cultura anual produtora de grãos, conhecida como pseudocereal devido à sua semelhança com a composição dos grãos de cereais. Suas sementes são consumidas mundialmente devido as suas propriedades nutricionais, atualmente apresentando elevada demanda. Deste modo a cultura se apresenta como uma importante alternativa para diversificação de cultivos e geração de renda para pequenos agricultores. Atualmente seu consumo e produção estão difundidos ao redor do mundo. No Brasil, sua introdução é recente, sendo os primeiros estudos desenvolvidos na região central do Brasil. Devido ao promissor mercado de alimentos nutracêuticos, a produção deste pseudocereal apresenta grande importância. Tendo em vista a necessidade de estudos que viabilizem a produção de grãos e sementes, o objetivo do presente trabalho é avaliar a influência das condições ambientais nos caracteres morfológicos e agronômicos e qualidade fisiológica de sementes.. Para tal foi efetuada a análise de crescimento da cultura, foram obtidos os caracteres agronômicos e morfológicos da cultura e foi efetuada a adaptação de um teste para determinação de vigor pela quantificação da emissão de etanol pelas sementes. Através da curva de crescimento e analise da qualidade da semente produzida, evidenciou-se que não existe diferença significativa entre a cultivar brasileira e o acesso de origem andina, tal fato demonstrando a adaptabilidade da cultura na região. Através do estudo de caracteres morfológicos e agronômicos, observou-se que a utilização de formula adaptada para calculo de potencial de produção é viável para a cultura, existindo acentuada variabilidade entre os genótipos estudados. O estudo dos caracteres morfológicos indica que é possível diferenciar os genótipos de quinoa, especialmente levando em consideração o número de dentes na folha, entretanto observou-se grande variabilidade genética dentro de uma mesma cultivar. Através do estudo do metabolismo do etanol, observou-se relação significativa entre a sua produção e o nível de vigor de sementes, assim utilizando metodologia adaptada, foi possível diferenciar pela produção de etanol lotes de sementes de quinoa com diferentes níveis de vigor, assim comprovando a eficiência do teste. / Quinoa is a plant of the Chenopodiacea family, domesticated by the Andean peoples for thousands of years. It is an annual grain crop, known as pseudocereal due to its similarity to the composition of cereal grains. Its seeds are consumed worldwide due to their nutritional properties, currently presenting high demand. In this way culture is presented as an important alternative for crop diversification and income generation for small farmers. Currently its consumption and production are widespread around the world. In Brazil, its introduction is recent, being the first studies developed in the central region of Brazil. Due to the promising nutraceutical food market, the production of this pseudocereal is of great importance. The objective of the present work is to evaluate the influence of the environmental conditions on the morphological and agronomic traits and the physiological quality of the seeds. In order to do this, the analysis of crop growth , The agronomic and morphological characters of the crop were obtained and a test was performed to determine vigor by quantifying the ethanol emission by the seeds. Through the growth curve and analysis of the quality of the seed produced, it was evidenced that there is no significant difference between the Brazilian cultivar and the access of Andean origin, this fact demonstrating the adaptability of the crop in the region. Through the study of morphological and agronomic characters, it was observed that the use of a formula adapted to calculate potential production is feasible for the crop, and there is a marked variability among the studied genotypes. The study of the morphological characters indicates that it is possible to differentiate the quinoa genotypes, especially taking into account the number of teeth in the leaf, but great genetic variability was observed within the same cultivar. Through the study of the ethanol metabolism, it was observed a significant relationship between its production and the level of seed vigor, thus using adapted methodology, it was possible to differentiate by the ethanol production lots of quinoa seeds with different levels of vigor, thus proving The efficiency of the test.

Background and objectives: The commonly used afrosimetric foam-height method for quantifying saponins in quinoa is rapid but imprecise. A rapid UV/Vis method, utilizing the Liebermann-Burchard (LB) color reaction, was compared to the foam method across a range of saponin levels in washed and unwashed seed. Findings: A 6 min UV/Vis method provided greater precision and accuracy than the afrosimetric method at the lower saponin levels found in washed quinoa. The afrosimetric method did not differentiate saponin levels below 0.6 mg/mL but allows for useful relative comparisons of saponin content in unwashed quinoa where the foam height is large. Conclusions: The UV/Vis method is superior for analyzing saponins present on washed quinoa seed, while the foam method is adequate for saponin measurements in unwashed seed. Significance and novelty: The UV/Vis assay provides a new tool that can be used for in-process quality control in large-scale processing facilities, allowing for rapid, accurate determination of completion of the desaponization step.

Chenopodium quinoa Willd., an ancient Andean pseudocereal almost exclusively consumed in South America, jumped onto the global stage when Western cultures noted quinoa's advantageous nutritional profile. Quinoa seed's high protein content, nutritionally balanced amino acid profile, low glycemic index, and high fiber, vitamin, and mineral content, make it a highly sought-after 'superfood'. Pitseed goosefoot (C. berlandieri Moq.), a closely related North American species sharing quinoa's genome composition (AABB), grows across the North American continent, inhabiting diverse environments including the saline coastal soils of the Gulf of Texas and the drought-prone regions of the Southwest. Quinoa and pitseed goosefoot, along with South American avian goosefoot (C. hircinum Schrad.), make up the Allotetraploid Goosefoot Complex (ATGC). We hypothesize that an ancient hybridization event between A- and B-genome diploids, with a subsequent whole-genome duplication, gave rise to the common ancestor of the ATGC. Prior data indicate that allopolyploidization most likely occurred within North America, with long-range dispersal of the ATGC to South America. We have sequenced the genome of the North American AA-genome diploid C. watsonii and identified via DNA marker analyses the closest extant species to the AA-genome diploid ancestor of the ATGC from among a panel of 41 AA-genome diploid resequenced accessions, encompassing 30 putative AA-genome diploid species, from North and South America. We also present evidence for reciprocal long-range dispersal of Chenopodium diploids between North and South America.

Chenopodium quinoa, a South American pseudocereal, has valuable agricultural traits such as salt tolerance and drought tolerance, and it has beneficial nutritional properties such as high protein content and a complete amino acid profile. However, problems including disease susceptibility, low harvest index, lodging, seed shattering, low heat tolerance, and saponin content plague quinoa. Genetic resources for quinoa are needed to fix these problems and make quinoa more available throughout the world. We used ethyl methanesulfonate (EMS) to create a mutant population of QQ74 quinoa (USDA GRIN PI 614886) of 5,030 mutant families. We did whole exome sequencing (WES) on 44 mutant families. Using the recently published quinoa reference genome and MAPS, a mutation detection pipeline, we found a mutation rate of 11.35 mutations/Mb in these families. We also used whole genome sequencing (WGS) to calculate a mutation rate of 21.67 mutations/Mb in an additional nine mutant families. To demonstrate the utility of this population as a genetic resource, we found an EMS-induced nonsense mutation in the betalain synthesis pathway that prevents red betacyanins from accumulating in the hypocotyl of quinoa. With the mutation rates in our population, we calculate that analysis of 300 mutant families will yield 3-7 mutations in any gene of interest, which will facilitate forward and reverse genetic studies in quinoa.

Quinoa (Chenopodium quinoa Willd.) is an important food crop for subsistence farmers in the Altiplano (high plains) of Peru, Bolivia, and Argentina. Saponins are part of a diverse family of secondary metabolites that are found in high concentrations in the pericarp of many varieties of quinoa. Due to their bitter taste and anti-nutritive properties, saponins must be removed before the quinoa grain is consumed. There are ‘sweet’ varieties of quinoa that have significantly reduced levels of saponin. Previous research suggests saponin production is controlled by a single locus. The major objective of this research was to elucidate the genetic components in the saponin biosynthesis pathway. Thus, we report the development and annotation of the first large scale expressed sequence tag (EST) collection for quinoa based on Sanger and 454 pyrosequencing of maturing seed tissue expressing saponins. Sanger sequencing produced 18,325 reads with an average read length of 693 nucleotides, while 454 GS-FLX pyrosequencing generated 295,048 reads with an average read length of 202 nucleotides. A hybrid assembly of all sequences generated 39,366 unigenes, consisting of 16,728 contigs and 22,638 singletons. Repeat sequence analysis of the unigene set identified 291 new microsatellite markers. From the unigene set, a custom microarray was developed and used to assay transcriptional changes in developing seeds of saponin-containing and saponin-free quinoa lines. The microarray consisted of 102,834 oligonucleotide probes representing 37,716 sequences of the unigenes set. Three different statistical comparisons, based on comparisons of ‘sweet’ vs. ‘bitter’ seed tissue at two developmental stages, were assayed on the custom array. Using a p-value cutoff threshold of 0.01, we identified a list of 198 significantly differentially expressed candidate genes common to all three comparisons. We also identified a list of candidate genes (p-value ≤ 0.05) that are known to be associated with identified triterpenoid (saponin) biosynthetic pathways that were differentially expressed in all three comparisons. Included in this list are candidate genes that share homology to cytochrome P450s (20), cytochrome P450 monooxygenases (10), and glycosyltransferases (49) suggesting that transcriptional differences in the saponin biosynthesis pathway possibly responsible for the absence or presence of saponin in quinoa are determined after the formation of the β-amyrin skeleton. These candidate genes are suggested for use in future studies in the production of saponin in quinoa.

RNA-seq transcriptome analysis of Chenopodium quinoa at different water treatment levels was conducted in a greenhouse study using four water treatments (field capacity to drought) on a valley ecotype quinoa (variety Ingapirca) and an Altiplano Salares ecotype quinoa (variety Ollague). Physiological results support the earlier findings that the Salares ecotypes display greater tolerance to drought-like stress conditions than the valley ecotypes (as determined by growth rate, photosynthetic rate, stomatal conductance, and stem water potential). cDNA libraries from root tissue sample for each treatment x variety combination were sequenced using Illumina Hi-Seq technology in an RNA-seq experiment. De novo assembly of the transcriptome generated 20,337 unique transcripts. Gene expression analysis of the RNA-seq data identified 462 putative gene products that showed differential expression based on treatment and 27 putative gene products differential expressed based on variety x treatment, including significant increasing expression in the root tissue in response to increasing water stress. BLAST searches and gene ontology analysis show an overlap with drought tolerance stress and other abiotic stress mechanisms.

Dans les hauts plateaux des Andes entre le Pérou et la Bolivie, à 3 800 mètres d’altitude s’étend le lac Titicaca, berceau des civilisations précolombiennes et l’un des principaux centres mondiaux de domestication des espèces végétales cultivées pour l’agriculture. Cette région est reconnue comme le centre d’origine du quinoa, C. quinoa Willd. Elle concentre la plus grande diversité génétique du quinoa, tant pour les variétés paysannes cultivées que pour les espèces sauvages apparentées. Notre recherche a été conduite dans la région de Puno, Pérou, qui reste l’une des principales régions productrices de quinoa au monde. Le quinoa y présente une distribution spatiale selon un gradient climatique nord-sud et une différenciation en zones agroécologiques liée à l’altitude. Actuellement, sept principales espèces de parents sauvages du quinoa y sont présentes : C.ambrosioides L., C.incisum Poiret, C.pallidicaule Aellen, C.petiolare Kunth, C.hircinum Schrad., C.quinoa ssp. melanospermum Hunz. et C.carnosolum Moq. Cette diversité de ressources génétiques a une grande valeur pour l’évolution adaptative du quinoa notamment face aux effets du changement climatique. Notre thèse s’inscrit dans l’importance économique et culturelle du quinoa, étant à la fois une ressource alimentaire des régions andines et l’objet des marchés internationaux du fait de ses qualités nutritives. Cette opportunité économique peut avoir des impacts en termes de sécurité alimentaire, d’agrobiodiversité, et de gestion de l’agroécosystème. Cette thèse a analysé comment les communautés agricoles andines intègrent la présence des espèces de parents sauvages dans leurs pratiques de gestion et leurs pratiques agricoles autour du quinoa. Des cartographies participatives et des enquêtes ethnobotaniques ont été réalisées avec les membres de six villages choisis selon des critères biogéographiques. La modélisation chorématique a été appliquée à deux périodes, avant et après 1970, année charnière au Pérou pour l’agriculture, dans le but de montrer comment les dynamiques socio-spatiales du milieu andin se modifient, notamment en lien avec l’évolution de la culture du quinoa. La distribution des espèces de parents sauvages du quinoa apparaît fortement liée à l’organisation socio-spatiale de l’agroécosystème. Ces espèces sont maintenues par les villageois pour leurs multiples usages alimentaires, médicinaux et culturels, dans des espaces naturels, des zones pâturées, aux abords et également à l’intérieur des champs cultivés. Ceci est à la fois le résultat de la gestion dynamique organisée par les communautés rurales et des savoirs liés à ces espèces qui se transmettent de génération en génération. Cependant cette gestion est en train de changer sous la pression d’enjeux globaux liés au marché international du quinoa, dont les exigences impliquent de réduire la présence de parents sauvages dans les champs cultivés. En conclusion, la thèse aborde la durabilité des pratiques de gestion et des pratiques agricoles dans un objectif de conservation dynamique in situ de la biodiversité sauvage et cultivée. Une mise en perspective historique des résultats nous a permis de questionner l’évolution des pratiques de gestion de ces différentes espèces par les communautés locales. En termes d’implication, deux types de projets pourraient être réfléchis. Le développement de projets prenant en compte le maintien de la présence des parents sauvages du quinoa dans le champ cultivé est favorable à l’introduction de gènes d’intérêt pour aider le quinoa à s’adapter à des conditions écologiques changeantes sous les effets du changement climatique. Egalement, des projets spécifiques de conservation in situ de l’agrobiodiversité, qui considèrent l’espace naturel et l’espace cultivé comme un ensemble cohérent, représentent une voie de gestion de pools de gènes importante pour l’agriculture et l’alimentation mondiale / Lake Titicaca, the cradle of pre-Columbian civilizations and one of the world’s main centres of domestication for farmed plant species, lies 3,800 m above sea level in the central Andean Highlands between Peru and Bolivia. The region is acknowledged as the centre of origin of quinoa, Chenopodium quinoa Willd. The greatest genetic diversity of quinoa and its wild relatives is concentrated there. Our research was conducted in the Puno region (Peru), which remains one of the main quinoa producing regions in the world. Quinoa displays spatial distribution along a North-South climate gradient in the region, with differentiation into mostly elevation-related, agro-ecological zones that explain its genetic diversity. Seven main quinoa crop wild relatives currently exist there: C. ambrosioides L., C. incisum Poiret, C. pallidicaule Aellen, C. petiolare Kunth, C. hircinum Schrad., C. quinoa ssp. melanospermum Hunz. and C. carnosolum Moq. This diversity of plant genetic resources is of great value for the adaptive evolution of quinoa, especially under the effect of climate change. This PhD thesis deals with the economic and cultural importance of quinoa, which is both a food resource in the Andean regions and an international commodity due to its exceptional nutritional qualities (protein-rich). This economic opportunity may have impacts on local food security, agrobiodiversity and agro-ecosystem management. One way of investigating this issue is to examine how the distribution of crop wild relatives is linked to the way quinoa cultivation is spatially organized. The thesis analysed how Andean farming communities incorporate the presence of wild relatives in their quinoa-related management and farming practices. Participatory mapping and ethnobotanical surveys were carried out with members of six villages chosen according to biogeographical criteria along a North South gradient, combined with data related to elevation and the proximity to Lake Titicaca. In order to show how the socio-spatial dynamics of the Andean environment are changing, notably linked to changes in quinoa growing, chorematic modelling was applied to two periods, before and after 1970, which was a pivotal year for Peruvian farming (agrarian reform, territorial rights of indigenous communities). The distribution of quinoa crop wild relatives seems to be closely linked to how the agro-ecosystem is spatially organized. Local communities keep these species for their multiple food, medicinal and cultural uses in natural areas, grazing areas, around and in farmed fields. This results from the dynamic management organized by rural communities, and from knowledge of those species passed down through the generations, by both women and men. However, management is changing under the pressure of global challenges arising from the international quinoa market, which calls for fewer wild parents in farmed fields. To conclude, the thesis looks at the sustainability of management and farming practices with a view to dynamic in situ conservation of wild and cultivated biodiversity. Choremes are used to place the results in historical perspective, to see how the management of these different species by local communities is evolving. In terms of implications, two types of project could be considered. Developing projects that keep quinoa crop wild relatives in farmed fields promotes the introduction of genes of interest, helping quinoa to adapt to ecological conditions being modified by climate change. Likewise, specific projects for in situ conservation of agrobiodiversity, which consider natural and cultivated areas as a coherent whole, are a way of managing gene pools that is important for agriculture and for feeding the world.

Submitted by Maria Beatriz Vieira (mbeatriz.vieira@gmail.com) on 2017-06-22T16:33:35Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) dissertacao_gabriele_espinel_avila.pdf: 937823 bytes, checksum: 425729d85c0200d3520fa3c39b5b7aae (MD5) / Approved for entry into archive by Aline Batista (alinehb.ufpel@gmail.com) on 2017-06-22T20:38:09Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) dissertacao_gabriele_espinel_avila.pdf: 937823 bytes, checksum: 425729d85c0200d3520fa3c39b5b7aae (MD5) / Made available in DSpace on 2017-06-22T20:38:09Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) dissertacao_gabriele_espinel_avila.pdf: 937823 bytes, checksum: 425729d85c0200d3520fa3c39b5b7aae (MD5) Previous issue date: 2015-09-18 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Classificada como halófita facultativa, a quinoa (Chenopodium quinoa Willd.) é reconhecida por tolerar elevados níveis de salinidade. No Brasil, a cv. BRS Piabiru é a primeira recomendada para o cultivo granífero, constituindo alternativa para diversificação dos sistemas agrícolas. Contudo, estudos relativos à adaptação dessa cultivar no estado do Rio Grande do Sul, bem como às condições de salinidade ainda são necessários. Portanto, o objetivo deste trabalho foi avaliar as respostas fisiológicas (pigmentos e trocas gasosas) e bioquímicas (atividade enzimática antioxidante, teores de peróxido de hidrogênio, peroxidação lipídica, prolina e potencial osmótico) de plantas de quinoa cv. BRS Piabiru cultivadas sob condições de salinidade. O experimento foi conduzido em casa de vegetação onde as sementes foram distribuídas em vasos plásticos, preenchidos com areia lavada. Após sete dias da germinação foi fornecida solução nutritiva, ministrada a cada quatro dias e, aos 30 dias, foi realizado o desbaste, mantendo-se quatro plantas por vaso. Neste período também foram aplicadas as soluções salinas, na forma de cloreto de sódio, nas concentrações de 100, 200, 300 e 400 mM, além do tratamento controle cuja as plantas foram cultivadas na ausência da salinidade. As soluções nutritiva e salina foram aplicadas intercaladamente, a cada dois dias, até o fim do experimento. Aos 30, 60 e 90 dias após indução dos tratamentos, as plantas foram avaliadas quanto a parâmetros fisiológicos e bioquímicos (análises não destrutivas e destrutivas, respectivamente). Os dados foram submetidos à análise de variância (p≤0,05), as médias comparadas pelo teste de Tukey ao nível de 5% de probabilidade de erro e analisados por regressão polinomial. Houve redução no índice de clorofila e índice de balanço de nitrogênio e, a partir de 60 dias, incremento no índice de flavonoides. Neste período, a taxa de assimilação líquida de CO2 seguiu tendência quadrática de elevação até a concentração de 250 mM e as variáveis eficiência de uso da água e eficiência de carboxilação aumentaram linearmente com o incremento da salinidade. A atividade enzimática antioxidante, através das enzimas superóxido dismutase, catalase e ascorbato peroxidase, demonstrou que a salinidade promoveu adaptação e tentativa de neutralização das espécies reativas de oxigênio produzidas durante o estresse salino, tanto na parte aérea quanto em raízes. Houve aumento no potencial osmótico em ambos órgãos aos 90 dias após indução dos tratamentos, contudo, nas raízes a síntese de prolina não seguiu a mesma tendência. Apesar da redução nos índices de clorofila e de balanço de nitrogênio, há manutenção da taxa fotossintética, a qual, associada a capacidade antioxidante e ao ajuste osmótico até a concentração de 250 mM de NaCl, caracteriza a cv. BRS Piabiru com potencial de cultivo em solos sujeitos a salinização. / Classified as optional halophyte, quinoa (Chenopodium quinoa Willd.) is recognized for tolerate high levels of salinity. In Brazil, the cv. BRS Piabiru is the first recommended for grain production, providing an alternative for diversification of agricultural systems. However, studies on the adaptation of this variety in the state of Rio Grande do Sul, as well as the salinity conditions are still needed. Therefore, the objective of this study was to evaluate the physiological responses (pigments and gas exchange) and biochemical (antioxidant enzyme activity, hydrogen peroxide levels, lipid peroxidation, proline and osmotic potential) of quinoa plants cv. BRS Piabiru cultivated under saline conditions. The experiment was conducted in a greenhouse where the seeds were distributed in plastic pots filled with washed sand. After seven days of germination was supplied nutrient solution administered every four days and at day 30, the trimming was carried out, keeping four plants per pot. During this period the salt solutions have also been applied in the form of sodium chloride at concentrations of 100, 200, 300 and 400 mM, beyond which the control treatment plants were grown in the absence of salinity. The nutrient and saline solutions were applied interchangeably, every two days until the end of the experiment. At 30, 60 and 90 days after induction of treatments, the plants were evaluated for physiological and biochemical parameters (non-destructive and destructive analysis, respectively). Data were subjected to analysis of variance (p≤ 0.05), the averages compared by Tukey test at 5% error probability and analyzed by polynomial regression. There was reduction in chlorophyll content and nitrogen balance index and from 60 days, an increase in flavonoid content. During this period, net assimilation rate of CO2 followed quadratic upward trend until the concentration of 250 mM and the conditions of water use efficiency and carboxylation efficiency increased linearly with increasing salinity. The antioxidant enzyme activity by the enzyme superoxide dismutase, catalase and ascorbate peroxidase showed that the salinity promoted adaptation and attempt to neutralize the reactive oxygen species produced during salt stress, both in shoots and in roots. There was an increase of osmotic potential in both organs 90 days after induction of treatment, however, the roots proline synthesis did not follow the same trend. Despite the reduction in levels of chlorophyll and nitrogen balance, there is maintenance of photosynthetic rate, that, associated with antioxidant capacity and osmotic adjustment, until the concentration of 250 mM of NaCl, featuring the cv. BRS Piabiru with growing potential subjects soil salinization.

Nell’ambito della ricerca di colture alternative e di alimenti ad alto valore nutrizionale, presso il Dipartimento di Scienze delle Produzioni Vegetali Sostenibili, si è svolta un’attività di ricerca sull’adattabilità della quinoa in Italia Settentrionale. La sperimentazione è durata tre anni ed ha preso in considerazione areali pedo-climatici di pianura e di collina. Il primo obiettivo era quello d’identificare le varietà di quinoa più adatte alle condizioni ambientali del Nord Italia, e come secondo obiettivo individuare la tecnica di coltivazione appropriata per gli agrosistemi intensivi; come terzo obiettivo contribuire al miglioramento della tecnica di coltivazione della quinoa nei Paesi emergenti (Colombia). In Italia settentrionale le attività di ricerca sono iniziate nel 2014 con un confronto varietale di 24 ecotipi di quinoa. Per il ciclo colturale 2015, sono state scelte le migliori 11 cultivar delle prove precedenti, insieme ad altre 5 nuove cultivar. Per quanto riguarda invece il 2016, le prove sono state divise in due: 1. Due prove riguardanti alla scelta varietale 2. Una prova con lo scopo di studiare la risposta produttiva della quinoa alla fertilizzazione azotata. Per quanta riguarda la sperimentazione nei Paesi in Via di sviluppo sono state allestite due prove agronomiche nel dipartimento di Boyacà (Colombia). / With the aim of finding alternative crops and foods high in nutrients, at the Department of Sustainable Crop Production (DI.PRO.VE.S.), a research on the adaptability of quinoa in Northern Italy was carried out. This activity has continued for three years both in the plain and in the hills. The first aim of this work was to identify quinoa varieties most suitable for Northern Italy environmental conditions and as a second purpose to identify the appropriate cultivation technique to adopt the quinoa crop in the sustainable intensive farming systems; the third aim was to contribute to the improvement of quinoa cultivation technique in emerging countries (Colombia). In Northern Italy the research activities began in 2014 with a varietal comparison of 24 quinoa ecotypes. During 2015 crop cycle, the best 11 varieties out of the previous trials were selected, together with 5 new cultivars. In the third year (2016), the trials were divided into: 1. Regarding the varietal screening, two different trials 2. In order to study the response of quinoa to nitrogen fertilization, a trial a split-plot trial was arranged. Furthermore, regarding the research in the developing countries, two agronomic trials were established in the Department of Boyacà (Colombia).