Dans le cadre d’une convention de thèse CIFRE en collaboration de recherche entre la société Pollenergie et le Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), le potentiel antifongique de la propolis a été étudié. A cet effet, six échantillons de propolis provenant de régions géographiques différentes (France et Brésil) et d’origines botaniques variées (genre Populus, Dalbergia ecastophyllum et Baccharis dracunculifolia) ont été analysés.Les Extraits Ethanoliques de Propolis (EEP) mis au point, riches en composéspolyphénoliques (de 12,8 ± 0,4 à 16,2 ± 0,3 g EAG.l-1) présentent une grande diversité de classes de molécules bioactives selon leur origine botanique. Les polyphénols identifiés dans les extraits de propolis de Populus (France) sont principalement des flavonoïdes et leurs dérivés (galangine, pinobanksine, chrysine) et des acides-phénols (acides p-coumarique et caféique) et dans les extraits de propolis verte de Baccharis dracunculifolia et rouge de Dalbergia ecastophyllum (Brésil) ce sont respectivement des acides-phénols et leur dérivés prénylés (artépilline C, acide p-coumarique) et des composés isoflavonoïdiques (vestitol, médicarpine).Tous les EEP ont démontré un potentiel antifongique in vitro, variable selon les espèces botaniques, à deux stades de développement d’une souche phytopathogène (Colletotrichum musae), isolée de la pourriture de couronne de la banane. Deux extraits de Populus (France) en particulier ont montré la plus forte efficacité contre C. musae.Un extrait aqueux de propolis rouge de Dalbergia ecastophyllum a démontré la plus grande efficacité in vitro. Cet extrait, testé ensuite in vivo sur trois maladies de conservation de la banane a prouvé tout son potentiel antifongique comme traitement alternatif et son efficacité en augmentant la durée de conservation des bananes à l’export. / In the framework of a CIFRE thesis agreement in collaboration between Pollenergie and the Center for International Cooperation in Agronomic Research for Development (CIRAD), the antifungal potential of propolis has been studied. For this purpose, six samples of propolis from different geographical regions (France and Brazil) and various botanical origins (genus Populus, Dalbergiaecastophyllum and Baccharis dracunculifolia) were analyzed.The Ethanolic Extracts of Propolis (EEP) finalized, rich in polyphenolic compounds (from 12.8 ± 0.4 to 16.2 ± 0.3 g EAG.L-1), exhibit a great diversity of classes of bioactive molecules according to their botanical origin. The polyphenols identified in propolis extracts of Populus (France) are mainlyflavonoids and their derivatives (galangin, pinobanksin, chrysin) and acids-phenols (p-coumaric and caffeic acids). In contrast, the extracts of green propolis of Baccharis dracunculifolia and red from Dalbergia ecastophyllum (Brazil) they are respectively phenol acids and their prenylated derivatives(artepillin C, p-coumaric acid) and isoflavonoid compounds (vestitol, medicarpin).All EEPs have demonstrated an in vitro antifungal potential, which varies according to botanical species, at two stages of development of a phytopathogenic strain (Colletotrichum musae), isolated from the crown rot of banana. Two extracts of Populus (France) in particular showed the greatest efficacy against C. musae.An aqueous extract of red propolis from Dalbergia ecastophyllum demonstrated the greatest efficiency in vitro. This extract, which was then tested in vivo on three banana conservation diseases, proved its antifungal potential as an alternative treatment and its effectiveness in increasing the shelf life of bananas for export.

L'anthracnose constitue pour plusieurs productions végétales une maladie importante qui engendre des pertes post récoltes considérables. Colletotrichum musae est le responsable de cette maladie chez la banane dessert. L'utilisation d'antimicrobiens naturels comme le système lactoperoxydase (LPS) représente une voie naturelle de lutte intéressante contre l'anthracnose. Dans cette étude, nous avons amélioré le LPS en ajoutant de l'iode dans le système existant ou en substituant le thiocyanate par de l'iode. La substitution du thiocyanate à l'iode a permis d'avoir un effet fongicide du LPS. De plus, d'autres substances comme la lactoferrine, le Bioxeda® et l'huile de Neem ont été étudiées pour leur effet antifongique. Les deux dernières substances ont donné une inhibition supérieure respectivement à 90% et à 40%., tandis qu'il n'y a eu aucun effet de la lactoferrine / Postharvest diseases are a major concern for several plant products, leading to considerable postharvest losses. Colletotrichum musae is responsible for anthracnose and is also involved in crown rot, the two main postharvest diseases of bananas. The use of natural anti-microbial agents such as the lactoperoxidase system (LPS) represents an interesting alternative to the use of fungicides for the control of postharvest diseases of bananas. This study consisted on optimization of the LPS by adding iodide or substituting the thiocyanate by iodide. Moreover, other substances like lactoferrin, Bioxeda® and Neem oil were analyzed for their antifungal effect. The last two compounds gave an inhibition higher than 90% and 40% respectively. No effect of lactoferrin was observed

Fruit rot caused by Aspergillus niger or Colletotrichum musae is an important post-harvest disease on orange, chilli and Cavendish banana fruits. The use of synthetic fungicides has been a traditional strategy for the management of the fruit rot disease, but these chemicals adversely affect human health and environment. Therefore, the objective of this study was to evaluate the effects of CaCl2 on in vitro hyphal growth and in vivo lesion inhibition. First, aqueous solutions of CaCl2 at three concentrations of 20, 40 and 60 mM were assessed for their inhibitory effect against hyphal growth in vitro. Next, mature fruits were immersed into a solution of 20 mM CaCl2 for 20 - 30 s, then inoculated by a pathogen suspension at the density of 106 conidia mL-1 and observed for 12 days. The results showed that 20 mM CaCl2 was the most effective concentration in antifungal assay to Aspergillus isolated from orange rot. The treatment of CaCl2 continued to gain efficacy on limiting lesions’ development on orange fruits until 12 days after inoculation (DAI). On chilli, CaCl2 at concentrations of 20 and 40 mM inhibited well on the growth of Aspergillus hyphae isolated from chilli rot. However, calcium treatment was not effective on chilli fruits. On Cavendish banana, solutions of CaCl2 at concentrations of 20, 40 and 60 mM highly limited fungal growth of Colletotrichum in vitro conditions. The application of CaCl2 solution could inhibit anthracnose lesion length of Cavendish banana variety, but its efficacy did not prolong until 6 DAI. In general, the good results were obtained from the 20 mM CaCl2 in almost all the studied assays. Management of rot diseases on fruits by employing 20 mM CaCl2 could be suitable to replace the current hazardous agro-chemicals. / Thối trái do nấm Aspergillus niger hay nấm Colletotrichum musae là bệnh sau thu hoạch thường gặp trên cam, ớt và chuối già. Thuốc trừ nấm tổng hợp là biện pháp truyền thống quản lý bệnh thối trái nhưng lại ảnh hưởng bất lợi đến sức khỏe con người và môi trường. Vì vậy, mục tiêu của nghiên cứu là đáng giá ảnh hưởng của CaCl2 đối với sự sinh trưởng in vitro của nấm và sự ức chế vết bệnh ở điều kiện in vivo. Đầu tiên, dung dịch CaCl2 ở các nồng độ 20, 40 và 60 mM được sử dụng để đánh giá khả năng ức chế sự sinh trưởng in vitro của nấm bệnh. Tiếp theo, trái trưởng thành được nhúng vào dung dịch CaCl2 20 mM trong 20 - 30 s, rồi lây nhiễm với huyền phù mầm bệnh ở mật số 106 bào tử mL-1 và quan sát đến 12 ngày. Kết quả cho thấy CaCl2 20 mM có hiệu quả ức chế tốt đối với nấm Aspergillus phân lập từ bệnh thối trái cam. CaCl2 tiếp tục thể hiện hiệu quả ức chế bệnh trên trái cam đến 12 ngày sau lây bệnh. Trên ớt, CaCl2 20 và 40 mM cho hiệu quả ức chế sự phát triển nấm Aspergillus phân lập từ bệnh thối trái ớt. Tuy nhiên, xử lý CaCl2 không mang lại hiệu quả mong đợi trên trái ớt. Trên chuối già, dung dịch CaCl2 ở các nồng độ 20, 40 và 60 mM ức chế tốt sợi nấm Colletotrichum trong điều kiện in vitro. Dung dịch canxi có thể ức chế vết bệnh thán thư trên chuối già, nhưng hiệu quả không kéo dài đến 6 ngày sau lây bệnh. Nhìn chung, các kết quả tốt đều đạt được khi xử lý bằng CaCl2 20 mM ở hầu hết các thí nghiệm. Việc kiểm soát bệnh thối trái bằng CaCl2 20 mM có thể thay thế cho hóa chất nông nghiệp độc hại hiện nay.

L'anthracnose constitue pour plusieurs productions végétales une maladie importante qui engendre des pertes post récoltes considérables. Colletotrichum musae est le responsable de cette maladie chez la banane dessert. L'utilisation d'antimicrobiens naturels comme le système lactoperoxydase (LPS) représente une voie naturelle de lutte intéressante contre l'anthracnose. Dans cette étude, nous avons amélioré le LPS en ajoutant de l'iode dans le système existant ou en substituant le thiocyanate par de l'iode. La substitution du thiocyanate à l'iode a permis d'avoir un effet fongicide du LPS. De plus, d'autres substances comme la lactoferrine, le Bioxeda® et l'huile de Neem ont été étudiées pour leur effet antifongique. Les deux dernières substances ont donné une inhibition supérieure respectivement à 90% et à 40%., tandis qu'il n'y a eu aucun effet de la lactoferrine

Made available in DSpace on 2017-07-10T18:01:58Z (GMT). No. of bitstreams: 1 Eliete Moura de Souza Hurmann.pdf: 819870 bytes, checksum: 4302d144fe2194c2bcd5c074b31e3635 (MD5) Previous issue date: 2016-01-08 / Fundação Araucária / Trichoderma spp. is a promising antagonist, the development and use of products based on this organism gives us the opportunity not only to reduce health risks, but also costs and environmental damage. This work aimed to analyze the efficiency of Trichoderma viride extracts and Trichoderma stromaticum against some microorganisms of interest in clinical medicine, agriculture and fish farming. Among them Colletotrichum musae, banana anthracnose causes, Saprolegnia, which affects fish eggs and some bacteria that cause harm to human health. The dichlorometane extracts were tested at various concentrations, and as positive control a commercial antimicrobial. Inhibition of the pathogen was verified directly by paired cultivation technique. The antimicrobial activity of the extracts was evaluated by disk diffusion and the determination of minimum inhibitory concentration (MIC) by microdilution test broth. In situ tests were done in the fruit inoculating the pathogenic fungus and treated with the extracts and the sensory analysis where it was determined the acceptance of the product. In cultivation paired the Trichoderma spp. inhibited the growth of pathogens being 0.05% significance level. In the disk diffusion test results were positive, and for E. coli and Aeromonas hydrophila gave the best results. MIC against microorganisms of the extracts ranged from 50% to 3,125%. Given the results presented, it is concluded that the extracts were effective in in vitro inhibition of the microorganisms as well as their application in the fruits did not alter the organoleptic characteristics. / O Trichoderma spp. é um antagonista promissor, o desenvolvimento e uso de produtos à base deste microrganismo nos oferece a oportunidade, não apenas de reduzir os riscos da saúde, mas também custos e danos ambientais. Assim, este trabalho teve por objetivo analisar a eficiência dos extratos de Trichoderma viride e Trichoderma stromaticum contra alguns microrganismos de interesse na clínica médica, agricultura e piscicultura. Dentre eles o Colletotrichum musae, causador da antracnose da banana, Saprolegnia, que acomete ovas de peixes e algumas bactérias que causam danos à saúde humana. Os extratos diclorometânicos foram testados em várias concentrações, tendo como controle positivo um antimicrobiano comercial. A inibição do patógeno foi verificada, de forma direta pela técnica de cultivo pareado. A atividade antimicrobiana dos extratos foi avaliada por disco-difusão e pela determinação da concentração inibitória mínima (MIC) por teste de microdiluição em caldo. Foram feitos testes in situ no fruto inoculando o fungo patogênico e tratados com os extratos e a análise sensorial onde foi determinada a aceitação do produto. No cultivo pareado os Trichoderma spp. inibiram o crescimento dos patógenos sendo 0,05% de significância. No teste de disco-difusão os resultados foram positivos, sendo que para Aeromonas hydrophila e E. coli obteve-se os melhores resultados. O MIC (concentração inibitória mínima)dos extratos contra os microrganismos variou de 50% a 3,125 %. Diante dos resultados apresentados, evidenciou-se que, os extratos foram eficientes na inibição in vitrodos microrganismos testados, bem como sua aplicação nos frutos não alterou as características organolépticas dos mesmos.

Submitted by (lucia.rodrigues@ufrpe.br) on 2017-03-23T15:49:15Z No. of bitstreams: 1 Wagner Rogerio Leocadio Soares Pessoa.pdf: 1202325 bytes, checksum: 24359deb95d67f77dc4011b48213d142 (MD5) / Made available in DSpace on 2017-03-23T15:49:15Z (GMT). No. of bitstreams: 1 Wagner Rogerio Leocadio Soares Pessoa.pdf: 1202325 bytes, checksum: 24359deb95d67f77dc4011b48213d142 (MD5) Previous issue date: 2005-02-28 / Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq / The banana is the second largest fruit more consumed in the world, being the fresh fruit that detain the largest market. Brazil blunts as second largest producer of the fruit. In Brazil the varieties Silver and Pacovan approximately 60 % of the cultivate area. However, several phytopathological factors such as fungi and bacteria can cause the banana crop losses that could reach up to 40 %. Among these factors, anthracnose caused by Colletotrichum musae, deserve special attention in harming the commercialization and the consumption in natura. Because of the importance of this disease in banana and the lack of information on the subject, this work had two objectives: 1) study the influence of inoculation methods, different temperatures associates to different wetness period on the development of the disease; 2) verify alterations physicalchemistry happened in the banana fruits. The fruits were inoculated with 17 isolated of C. musae in two ways using PDA disk containing structures of the pathogen and suspension of conidia 4x 106 conidia / mL. The banana fruits were inoculated with the pathogen in the presence or not of artificial wounds. All C. musae isolates were pathogenic independently of the inoculation method used. Lesions could be observed in artificially wounded fruits independently of the inoculation method used (disk and suspension). In order to access the influence of temperature and wetness period, the isolates MAG2, SFV1 and FSA of C. musae were used isolated. These isolates were chosen because they show a wide rage of aggressiveness in the pathogenicity test. The bananas fruits were inoculated and incubated in the temperatures of 10, 15, 20, 25 and 30 oC with periods of wetness of 0, 12, 24 and 36 h, during five days. Temperatures of 20, 25 and 30 oC and with increasing wetness periods favored the development of lesions. Largest lesions wereobserved in the temperatures around 25 to 30 oC and 36 h of wetness period. Several physicalchemical alterations could be observed in the fruits during thexperiment: 1) there was an increase in the banana fruit humidity as the temperature and the wetness period increased, 2) They reach its highest pH value at 30 oC independently of the wetness period, 3) The largest ATT for the isolates SFV and MAG occurred at the same temperature of 25 oC, with wetness period of 12 and 24 h, respectively. The largest ATT value for the isolate FSA occurred at the temperature of 15 oC associated to the wetness period of 36 h, 4) Maximum SST occurred at temperature around 20 oC independent of the wetness period, 5) The largest potassium levels was reach at the temperature of 10 oC with wetness period of 24 h for SVF and MAG and without wetness period in FSA, 6) The total sugars content increased with the elevation of the temperature, decrease as the temperature approached of 30 oC, independent of the wetness period and the isolated of C. musae used. / A banana é a segunda fruta mais consumida no mundo, sendo (a fruta fresca neste contexto detentora de maior mercado no mundo) o Brasil segundo maior produtor, utilizando as variedades Prata e Pacovan em aproximadamente 60% de sua área cultivada. Entretanto, diversos fatores podem ocasionar perdas na produção, podendo chegar à ordem de 30 a 40%. Entre estes fatores encontram-se fungos e bactérias, merecendo destaque especial na pós-colheita a antracnose, causada por Colletotrichum musae, prejudicando a comercialização e o consumo in natura. Diante da importância desta doença na banana e a carência de informações sobre o assunto, este trabalho teve por objetivos, estudar a influência de métodos de inoculação, diferentes temperaturas associadas a distintos períodos de molhamento sobre o desenvolvimento da doença, e sua influência nas características físicoquímica. Avaliaram-se 17 isolados de C. musae, utilizando-se dois tipos de inóculo: disco de meio BDA contendo estruturas do patógeno e suspensão de conídios 4x106 conídios/mL, em duas formas de inoculação: com e sem ferimentos. Todos os isolados de C. musae mostraram-se patogênicos independente do tipo de inóculo utilizado. As lesões ocorreram nas frutas com ferimento, independente do tipo de inóculo (disco e suspensão). Para o estudo da influência da temperatura e período de molhamento, utilizaram-se três isolados do patógeno MAG2, SFV1 e FSA, por mostraram-se mais agressivo, intermediário e menos agressivo, respectivamente, no teste de patogenicidade. Asfrutas foram inoculadas e incubadas nas temperaturas de 10, 15, 20, 25 e 30oC e períodos de molhamento de 0, 12, 24 e 36 h, por cinco dias. As temperaturas em torno de 20, 25 e 30oC independente do período de molhamento favoreceram um maior desenvolvimento de lesões. As alterações físico-química mostraram que houve um aumento de umidade da fruta à medida que a temperatura e o período de molhamento aumentavam. Valores mais elevados de pH ocorreram em torno da temperatura de 30oC, independente do período de molhamento. Em relação a Acidez Titulável Total (ATT) para o isolado SFV1 e MAG2 ocorreu na temperatura ao redor de 25oC, com período de molhamento de 12 e 24 h, respectivamente, e para o isolado FSA ocorreu ao redor da temperatura de 15oC associado ao período de molhamento de 36 h. O teor de Sólidos Solúveis Totais (SST) ficou em volta de 20oC independente do período de molhamento. A temperatura ao redor de 10oC favoreceu a elevação no nível de potássio das frutas, associado ao período de molhamento de 24 h, em frutas inoculadas com os isolados SVF1 e MAG2, e 0 hora quando utilizou-se o isolado FSA. Os teores de açúcares totais aumentaram com a elevação da temperatura, declinando à medida que esta se aproximava de 30oC, independente do período de molhamento e isolados de C. musae.

Submitted by (lucia.rodrigues@ufrpe.br) on 2017-03-27T16:34:00Z No. of bitstreams: 1 Wagner Rogerio Leocadio Soares Pessoa.pdf: 794895 bytes, checksum: fa791ef69e88320cf4072254f9211112 (MD5) / Made available in DSpace on 2017-03-27T16:34:00Z (GMT). No. of bitstreams: 1 Wagner Rogerio Leocadio Soares Pessoa.pdf: 794895 bytes, checksum: fa791ef69e88320cf4072254f9211112 (MD5) Previous issue date: 2009-03-13 / Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq / The banana is the second fruit more consumed in the world, being the fruit fresh holder of larger world market. Brazil, answers as second producing adult, using the varieties Silver and Pacovan in approximately 60% of area harvestd. However, several factors can cause losses in the production. In the powder-crop to the anthracnose, caused by Colletotrichum musae, it is the main and more destructive, harming the commercialization. Before that, the present work concerns the evaluations of alternative methods of control that seek to the reduction of the losses powder-crop in bananas caused by the C. musae. The first work is had the effect of resistance inductors in the control powder-crop of the anthracnose in banana.Among the tested inductors they are had the Acibenzolar-S-methyl (ASM), Agriculture-Mós®, Ecolife®, Crop-Set®, methyl jasmonate, were applied in the dosage recommended by the manufacturer (DR) and DR added of 50% there is plus, for five minutes of immersion, the inoculations were accomplished under the times of zero, six and 12 hours after the induction. In the second rehearsal the production of the enzymes was evaluated: peroxidase, polifenoloxidase, quitinase, β-1,3-glucanase and β -1,4-glucanase under bananas induce with biotic elicitores and abióticos. The third experiment, concerns the thermotherapy (TH) associate to ASM in the control of the anthracnose. Where bouquets were treated by immersion in warm water to 40, 45, 50 and 55 ± 1 ºC, for the times of zero, five, 10 and 15 minutes, parallel to these treatments the bouquets were immersed in syrup containing ASM, for five minutes, in the DR for the manufacturer. In relation to the fourth work, the effect of dosages zero 50, 150, 300 and 450 nL.L-1 of 1-MCP on the banana anthracnose was verified. The inoculations of all of the works were accomplished with a suspension of conidial of C. musae in the concentration 106 con./mL, deposited on the epidermis previously wounded. At the end of each rehearsal they were appraised the banana's physiochemical characteristics except for the second. ASMapplied 12 hours before the inoculation in the added of 50% commercial dosage there is plus, it was the most efficient in the control of the disease. In relationship, to enzymes peroxidase, polifenoloxidase β -1,3-glucanase and β -1,4-glucanase, AGM and MJ the most efficient inductors were in the production of these enzymes, in relation to the quitinase AGM did just stand out in relation to the others. To the temperatures around of 40 and 45 ºC in all of the times of exhibition tested were the most expressive in the reduction of the severity in relationship á testifies. In TH + ASM, for the temperatures from 45 to 50 °C in all of the times of exhibition and 40 °C in the times of 10 and 15 minutes presented smaller severity of the disease in relation to isolated TH. In the 1-MCP, the dosages of 150 and 50 nL.L-1 they presented the smallest severity values with 9,57 and 9,67 mm, respectively, following for the dosage of 300 and 450 nL.L-1 with 10,18 to 10,5 mm. The witness presented the largest severity with 32,04 mm being reduced in the progressive largest way for to smallest dosage.It didn't happen significant changes in the pH, SST and ATT, that commit the commercialization and the banana's consumption in natura. / A banana (Musa spp.)é a segunda fruta mais consumida no mundo, sendo a fruta fresca detentora de maior mercado mundial. O Brasil, responde como segundo maior produtor, utilizando as cvs. Prata e Pacovan em aproximadamente 60 % de sua área cultivada. Contudo, diversos fatores podem ocasionar perdas na produção. Na pós-colheita à antracnose, causada por Colletotrichum musae, é a principal e mais destrutiva, prejudicando a comercialização. Diante disso, o presente trabalho tem por objetivo avaliar métodos alternativos de controle que visem à redução das perdas pós-colheita em bananas causada por C. musae. O primeiro artigo tem-se o efeito de indutores de resistência no controle pós-colheita da antracnose em banana. Foram avaliados os indutores acibenzlar-S-metil (ASM), Agro-Mós®, Ecolife®, Crop- Set®, metil jasmonato, sendo aplicado na dosagem recomendada (DR) pelo fabricante e DR acrescido de 50 %, por cinco minutos de imersão. As inoculações com o isolado Cm 10 foram realizadas sob os tempos de zero, seis e 12 horas após a indução. No segundo artigo, avaliou-se a produção das enzimas peroxidase, polifenoloxidase, quitinase, β-1,3-glucanase e β-1,4- glucanase sob bananas induzidas com elicitores bióticos e abióticos. O terceiro artigo, diz respeito ao tratamento hidrotérmico (TH) associado ao ASM no controle da antracnose. Onde buquês foram tratados por imersão em água aquecida a 40, 45, 50 e 55 ± 1 ºC, pelos tempos de zero, cinco, 10 e 15 minutos. Paralelo ao tratamento TH, os buquês foram imersos em calda contendo o ASM, por cinco minutos, na DR do fabricante. Em relação ao quarto artigo, verificou-se o efeito de dosagens 0, 50, 150, 300 e 450 nL.L-1 de 1-MCP sobre a antracnose da banana. As inoculações de todos os trabalhos foram realizadas com suspensão de conídios de C. musae na concentração de 106 con./mL, depositado sobre a epiderme previamente ferida. Ao final de cada ensaio foram avaliadas as características físico-químicas da banana com exceção do segundo experimento. O ASM e o AGM aplicado 12 horas antes dainoculação na dosagem comercial acrescido de 50 %, foram o mais eficientes no controle da doença. Em relação as enzimas peroxidase, polifenoloxidase, β-1,3-glucanase e β-1,4- glucanase, o AGM e o MJ foram os indutores mais eficientes em sua produção. Para a quitinase apenas o AGM destacou-se em relação aos demais. Às temperaturas ao redor de 40 a 45 ºC em todos os tempos de exposição testados foram as mais expressivas na redução da severidade da doença em relação á testemunha. No TH + ASM, para as temperaturas de 45 a 50 °C em todos os tempos de exposição e 40 °C nos tempos de 10 e 15 minutos apresentaram menor severidade da doença em relação ao TH isolado. No 1-MCP as dosagens de 150 e 50 nL.L-1 apresentaram os menores valores de severidade com 9,57 e 9,67 mm, respectivamente. Não ocorreram mudanças significativas no pH, SST e ATT, que comprometessem a comercialização e o consumo in natura da banana.