La chromatine sert de support à de multiples processus biologiques, cependant son organisation spatiale diffère fortement selon l'échelle considérée. L'expression des gènes est ainsi coordonnée par des éléments régulateurs dispersés dans le génome mais capables d'interagir entre eux. Chez les métazoaires, des expériences de capture de conformation de chromosome (3C) combinées au séquençage haut-débit (Hi-C) ont permis la découverte de domaines d'association topologique (TAD), à l'échelle de la mégabase. Puisque la résolution du Hi-C reste limitée, nous avons utilisé la 3C-qPCR pour explorer, dans des cellules souches embryonnaires murines, la dynamique chromatinienne à l'intérieur de ces domaines ainsi qu'à leurs bordures. Nous identifions ainsi une modulation des fréquences de contacts, sur quelques centaines de kilobases. Cette modulation est plus ou moins importante en fonction du contenu en gènes des domaines, mais elle semble néanmoins universelle. Des modèles dérivés de la physique des polymères permettent de décrire cette modulation sous la forme d'une hélice statistique, que la chromatine adopterait en moyenne et en l'absence d'interactions spécifiques, à l'intérieur des TAD. Cette hélice reflète certaines contraintes que la chromatine subit à l'échelle supranucléosomale. Elle est très affectée par les bordures, qui bloquent la modulation, mais elle l'est beaucoup moins par le contenu en histone de liaison H1. Par ailleurs, grâce à des résultats de Hi-C à haute résolution, nous montrons que la modulation observée chez les souris n'est pas retrouvée chez la drosophile, où les caractéristiques des TAD semblent avant tout liées au paysage épigénétique local. Pour ces deux organismes, la dynamique chromatinienne à l'intérieur des domaines est donc sous le contrôle de phénomènes différents / The chromatin hosts various biological processes. However, its organization differs considerably depending on the scale. For example, gene expression is coordinated by regulatory elements that are dispersed in the genome but that are able to interact within the tridimensional space of the nucleus. In the Metazoa, chromosome conformation capture (3C) assays combined with high-throughput sequencing (Hi-C) uncovered the existence of topologically associating domains (TADs), at the mégabase scale. Due to the limited resolution of Hi-C, we used the 3C-qPCR method to explore, in murine embryonic stem cells, the chromatin dynamics inside TADs as well as at their borders. We found that contact frequencies undergo a periodic modulation over large genomic distances (few hundred kilobases). This modulation is weaker in gene-deserts than in gene-containing domains but it seems nevertheless to be universal. Using models derived from polymer physics, we show that this modulation can be understood as a fundamental helix shape that chromatin tends to adopt statistically, when no strong locus-specific interaction takes place, within the TADs. This statistical helix reflects some constraints that the chromatin undergoes at the supranucleosomal scale. It is affected by TADs borders, which disrupt the modulation, but linker histone H1 depletion only leads to subtle changes in the helix characteristics. Furthermore, using high-resolution Hi-C data, we found that chromatin dynamics is unconstrained in Drosophila where it seems mainly linked to the local epigenetics landscape. Therefore, distinct genome organization principles govern chromatin dynamics within mouse and Drosophila topologically associating domains.

Armadilhamento duplo de átomos neutros constitui um dos avanços mais recentes no estudo de colisões frias. Obtivemos experimentalmente as taxas de perda devido a interação de espécies alcalinas mistas (Cs/Rb). Utilizamos uma armadilha magneto-óptica mista operando com feixes de alta intensidade permitindo o resfriamento e confinamento das amostras com sucesso. Esse trabalho apresenta-se de forma relevante pois corresponde a primeira etapa para o estudo do processo de formação de moléculas frias heteronucleares. Uma comparação entre duas técnicas de monitoramento dos processos de carga de átomos foi realizada. Os resultados tanto qualitativamente como quantitativamente foram satisfatórios. Determinamos as taxas de perda, de interesse e analisamos essas taxas considerando a proporção entre as massas dos átomos envolvidos e enfocando o efeito causado pela diferença de massa entre eles concluindo assim o objetivo principal dessa dissertação. / Double trapping of neutral atoms is one of the most recent advances in the study of collisions cold. Experimentally obtained the rates of loss due to the interaction of species alkaline mixed (Cs / Rb). We use a magneto-optical trap mixed operating with beams of high intensity allowing the cooling and containment of the samples successfully. This work is so important because it represents the first step to study the process of formation of molecules heteronucleares cold. A comparison between two techniques of monitoring procedures for cargo of atoms was performed. The results both qualitatively and quantitatively were satisfactory. And the rates of loss of interest and analyze the rates considering the ratio between the masses of atoms involved and focusing on the effect caused by the difference of mass between them thus completing the main objective of this dissertation.

La détermination d'abondances stellaires très précises a toujours été et reste un point clé de toute analyse spectroscopique.Cependant, de nombreuses études ont montré que l'hypothèse de l'équilibre thermodynamique local (ETL), largement utilisée dans les analyses spectroscopiques est inadéquate pour déterminer les abondances et les paramètres stellaires des étoiles géantes et pauvres en métaux où les effets hors-ETL dominent. C'est pourquoi, une modélisation hors-ETL des spectres stellaires est cruciale afin de reproduire les observations et ainsi déterminer avec précision les paramètres stellaires.Cette modélisation hors-ETL nécessite l'utilisation d'un grand jeu de données atomiques, qui ne sont pas toujours connues avec certitude. Dans les étoiles froides, les taux de collisions de l'atome d'hydrogène sont une des principales sources d'incertitudes. Ces taux sont souvent calculés en considérant une approche classique (l'approximation de Drawin) pour les transitions permises lié-lié et les transitions d'ionisations. Cette approche classique tend à surestimer les taux de collisions et ne reproduit pas correctement le comportement avec les énergies.Dans cette thèse, nous démontrons que l'approximation de Drawin ne peut pas décrire les taux de collisions dans le cas de l'atome d'hydrogène. Nous présentons une nouvelle méthode pour estimer ces taux, par le biais d'ajustement sur des taux quantiques existant pour d'autres éléments.Nous montrons que cette méthode d'ajustement quantique (MAQ) est satisfaisante pour les modélisations hors-ETL lorsque les taux quantiques dédiés ne sont pas effectivement disponibles.Nous testons cette nouvelle méthode, avec le modèle d'atome de Fer que nous avons développé, sur des étoiles de référence issues « du Gaia-ESO survey ».En partant de paramètres photosphériques non-spectroscopiques connus, nous déterminons les abondances (1D) en fer de ces étoiles de référence dans les cas ETL et hors-ETL .Nos résultats dans le cas hors ETL conduisent à un excellent accord entre les abondances de FeI et FeII avec de faibles écarts types de raies à raies, particulièrement dans le cas des étoiles pauvres en métaux.Notre méthode est validée par comparaison avec de nouveaux calculs quantiques préliminaires sur l'atome de Fe I et d'hydrogène, dont les ajustements sont en excellent accord avec les nôtres. / Determination of high precision abundances has and will always be an important goal of all spectroscopic studies. The use of LTE assumption in spectroscopic analyses has been extensively shown in the literature to badly affect the determined abundances and stellar parameters, especially in metal-poor and giant stars which can be subject to large non-LTE effects. Non-LTE modeling of stellar spectra is therefore essential to accurately reproduce the observations and derive stellar abundances. Non-LTE calculations require the inputof a bulk of atomic data, which may be subject to uncertainties. In cool stars, hydrogen collisional rates are a major source of uncertainty, which are often approximated using a classical recipe (the Drawin approximation) for allowed bound-bound, and ionization transitions only. This approximation has been shown to overestimate the collisional rates, and does not reproduce the correct behavior with energies. We demonstrate in this dissertation the inability of the Drawin approximation to describe the hydrogen collisional rates.We introduce a new method to estimate these rates based on fitting the existing quantum rates of other elements. We show that this quantum fitting method (QFM) performs well in non-LTE calculations when detailed quantum rates are not available. We test the newly proposed method, with a complete iron model atom that we developed, on a reference set of stars from the Gaia-ESO survey. Starting from well determined non-spectroscopic atmospheric parameters, we determine 1D, non-LTE, and LTE iron abundances for this set ofstars. Our non-LTE results show excellent agreement between Fe I and Fe II abundances and small line-by-line dispersions, especially for the metal-poor stars. Our method is validated upon comparison with new preliminary Fe I+H quantum calculations, whose fits show an excellent agreement with ours.

Les calculs théoriques prévoient que la dynamique d’excitation rotationnelle desmolécules CO et O2, induite par collision avec H2, est dominée par des résonancesquantiques aux très basses énergies. Leur mise en évidence expérimentale estrendue difficile par la nécessité d’obtenir des énergies de collision très faibles et unegrande résolution en énergie. Les expériences menées grâce à un montage defaisceaux moléculaires croisés à angle d’intersection variable, nous permettent ainsid’observer le seuil des transitions j = 0  1 de CO à 3,85 cm-1 et Nj = 10  11 de O2à 3,96 cm-1. Ces énergies correspondent à l’énergie cinétique moyenne d’un gaz àune température inférieure à 4 K. Les pics dans le tracé des sections efficacesintégrales en fonction de l’énergie de collision, constituent la première observationexpérimentale de résonances pour des processus inélastiques. Le bon accord avecles calculs théoriques permet de valider les potentiels d’interaction et ainsi dedéduire les constantes de vitesse pour la modélisation du milieu interstellaire. Nosrésultats expérimentaux mettent en relief la nature quantique des interactionsmoléculaires aux très basses énergies. / Theoretical calculations predict that the dynamics of rotational excitation of CO or O2molecules, induced by collisions with H2, are dominated by quantum scatteringresonances at very low energies. However, experimental observation of these effectsis challenging: very low collision energies and high energy resolution are bothrequired. Experiments performed with a crossed molecular beam apparatus withvariable intersection angle allow us to observe the thresholds of the CO (j = 0  1)transition at 3.85 cm-1 and the O2 (Nj = 10  11) transition at 3.96 cm-1, whichcorrespond to the average kinetic energy of a gas below 4 K. The peaks in theintegral cross section’s collision energy dependence constitute the first experimentalobservation of resonances in an inelastic process. The good agreement betweentheory and experiment reinforces the confidence in the interaction potentials used todeduce rate coefficients for modeling the interstellar medium in the 1-20 K range. Ourexperimental results highlight the quantum nature of molecular interactions at verylow energies.

With the use of an incoherent binary nucleon-nucleon collision model of heavy ion collisions for simulating particle production, it is demonstrated that the nuclear modification factors, R_{AA} and R_{CP}, are less than unity for hard scattering in the absence of any nuclear modification effects. The nuclear modification factor R_{dAu} is also shown to approach or exceed unity only if p_T broadening is taken into account. With a simple phenomenological parameter, the mean nucleon energy loss fraction, this model yields particle distributions that are comparable to those of experiment. The nuclear geometry is described by the Glauber model, and particle production is simulated by the PYTHIA event generator.

Spray drying is a widely used unit operation for producing particulate products directly from a liquid feed. Important processes that occur inside the spray dryer are droplet formation, droplet drying and interactions between droplets and recycled fines. Various studies have looked at the first two processes, but the latter phenomenon has received less attention. Literature on droplet-particle interaction which aims at quantitatively describing agglomeration in spray drying is scarce and mainly qualitative. For product quality the formation of agglomerates is often desirable. This thesis models and investigates the collisions of individual particles with single droplets. The surface tack of drying droplets has been identified as an important variable for the formation of agglomerates. In this thesis a novel method for measuring tack from the liquid phase has been further improved. The improvements are a more accurate load measurement, an automated control of the tack probe and an improved layout of the sample holder and probe. The key feature of the device is its ability to measure tack of drying droplets, whereas other devices measure tack by wetting a powder. Using our method the tack of a commonly spray dried product, yeast extract, has been measured. From these experiments it was found that with decreasing average moisture content the surface tack increases to a maximum. Below a critical average moisture content the surface of the droplet is dry and the tack rapidly decreases upon further drying. Another important parameter in determining the degree of agglomeration is the degree of penetration. If the particle penetrates the droplet too deeply, the agglomerate structure becomes too dense. To predict the penetration depth, a non-dimensional model has been developed. It describes the penetration of a particle into a liquid droplet during a head-on collision. It is based on a force balance and incorporates surface tension force, viscous force and capillary pressure force. The important parameters determining the collision outcome are the contact angle, the size of the droplet relative to the particle, the Reynolds and Weber numbers. For each contact angle an equilibrium penetration position exists, at this point the surface tension force vector is perpendicular to the penetration direction. Five different penetrations regimes are identified. At low Reynolds numbers, viscous forces dominate and the particle asymptotically travels towards the equilibrium position. Reducing the viscous drag force by increasing the Reynolds number results in initially overshooting the equilibrium position, but the surface tension force pulls the particle back, to attain the equilibrium in an oscillating motion. At even higher Reynolds numbers the particle fully penetrates the droplet, and reaches the centre of the droplet for even higher values for the Reynolds number. The ejection regime is found at high Reynolds number and low Weber numbers and the liquid should be non-wetting. Using the regime maps one is able to identify in which region a spray dryer is operating. Although the full penetration regimes are useful for capturing fines, it should be avoided when agglomeration is desired. The ejection regime should be avoided as well. To validate the model, impact experiments were carried out by dropping glass spheres on the surface of different liquids. These validation experiments were the first attempt to experimentally validate the collision of a single particle with a liquid surface. Besides yeast extract, which has non-Newtonian rheological properties, silicone oils with constant viscosities of 100 mPa•s and 1 Pa•s have been tested. The penetration over time for different impact velocities was determined by analysing high speed camera recordings. The typical penetration times ranged from 0.2 s to 2 s. To obtain accurate location data was recorded at frame rates up to 38 000 frames per second. Glass spheres, with a size of 2 mm were used to allow the visual tracking. Modelling the impacts showed that the model consistently predicted faster penetration times than were observed experimentally. The relative difference increased with increasing viscosity. A parameter fitting exercise showed that better agreement could be obtained by using a higher viscosity and a higher contact angle in the model. With this knowledge the most likely factor influencing the model-experiment mismatch was identified as being the dynamics of wetting of the particle surface. It was also found that using the dynamic contact angle in the model would improve its results. The non-Newtonian characteristics of the yeast extract resulted in the particle rebound and the formation of an air cavity upon impact. The tack measurement technique and penetration model presented in this thesis will be useful tools for the design of spray dryers. Recommendations are made for further model improvement. The experimental validation is the first attempt to validate the presented model. Future improvements are recommended and suggestions are presented.

No description available.

Armadilhamento duplo de átomos neutros constitui um dos avanços mais recentes no estudo de colisões frias. Obtivemos experimentalmente as taxas de perda devido a interação de espécies alcalinas mistas (Cs/Rb). Utilizamos uma armadilha magneto-óptica mista operando com feixes de alta intensidade permitindo o resfriamento e confinamento das amostras com sucesso. Esse trabalho apresenta-se de forma relevante pois corresponde a primeira etapa para o estudo do processo de formação de moléculas frias heteronucleares. Uma comparação entre duas técnicas de monitoramento dos processos de carga de átomos foi realizada. Os resultados tanto qualitativamente como quantitativamente foram satisfatórios. Determinamos as taxas de perda, de interesse e analisamos essas taxas considerando a proporção entre as massas dos átomos envolvidos e enfocando o efeito causado pela diferença de massa entre eles concluindo assim o objetivo principal dessa dissertação. / Double trapping of neutral atoms is one of the most recent advances in the study of collisions cold. Experimentally obtained the rates of loss due to the interaction of species alkaline mixed (Cs / Rb). We use a magneto-optical trap mixed operating with beams of high intensity allowing the cooling and containment of the samples successfully. This work is so important because it represents the first step to study the process of formation of molecules heteronucleares cold. A comparison between two techniques of monitoring procedures for cargo of atoms was performed. The results both qualitatively and quantitatively were satisfactory. And the rates of loss of interest and analyze the rates considering the ratio between the masses of atoms involved and focusing on the effect caused by the difference of mass between them thus completing the main objective of this dissertation.

L’expérience ALICE a pour principal objectif l’étude et la caractérisation du plasma de quarks et de gluons (QGP), un état de la matière nucléaire dans lequel les quarks et les gluons sont déconfinés. Les quarkonia constituent l’une des plus intéressantes sondes (des états liés d’un quark lourd Q et de son anti-quark Q) du QGP. De plus, l’étude de la production des quarkonia est très intéressante puisqu’elle peut contribuer à une meilleure compréhension de la Chromodynamique Quantique, la théorie décrivant l’interaction forte. La formation d’états de quarkonia lors de collisions hadroniques n’est pas bien comprise. Les deux principales approches théoriques décrivant la production d’états de quarkonia, le ‘Color Singlet Model’ (CSM) et la QCD non-relativiste (NRQCD), ont montré des difficultés à décrire simultanément la section efficace de production et la polarisation de tels états. Expérimentalement, les mesures de la polarisation des quarkonia n’ont pas toujours été compatibles entre elles. Ainsi, que ce soit du point de vue expérimental ou théorique, l’étude des quarkonia est restée inachevée. De nouvelles méthodes récemment proposées ont souligné la nécessité de mesurer tous les paramètres de la polarisation, dans les différents systèmes de référence. Dans ce contexte, de nouvelles mesures peuvent améliorer les contraintes actuelles, voire apporter de nouvelles contraintes sur les prédictions. ALICE a mesuré la polarisation du J/ψ lors de collisions pp à √8 = 7 TeV. La plus grande statistique des données à 8 TeV par rapport aux données à 7 TeV permet d’étendre les mesures à une gamme de pT plus large. Cette thèse présente une mesure complète de la polarisation de J/ψ, i.e. les trois paramètres de polarisation, dans deux systèmes de référence différents: le système Collins-Soper et le système d’hélicité. Les résultats ne montrent aucune polarisation significative pour le J/ψ dans le domaine cinématique étudié : 2.5 < y < 4.0 et 2 < pT < 15 GeV/c. Le paramètre invariant λ a également été mesuré afin d’écarter le risque d’un biais dans la procédure d’analyse. La comparaison de ces résultats avec les prédictions théoriques montre que la production de quarkonia n’est pas encore correctement décrite. Aucun de ces modèles théoriques n’est capable de décrire à la fois les mesures de sections efficaces et de polarisation. / The main purpose of the ALICE experiment is the study and characterization of the Quark Gluon Plasma (QGP), a state of nuclear matter in which quarks and gluons are deconfined. Quarkonia (bound states of a heayvy quark Q and its anti-quark Q) constitute one of the most interesting probes of the QGP. Besides this motivation, the study of quarkonium production is very interesting since it can contribute to our understanding of Quantum Chromodynamics, the theory of strong interactions. The formation of quarkonium states in hadronic collisions is not yet completely understood. The two main theoretical approaches to describe the production of quarkonium states, the Color Singlet Model and the Non-Relativistic QCD framework (NRQCD), have historically presented problems to simultaneously describe the production cross section and polarization of such states. On the experimental side, quarkonium polarization measurements have not always been complete and consistent between them. So, neither from the theoretical nor from the experimental point of view the situation was clear.Improved methods for the measurement of quarkonium polarization have been recently proposed, highlighting the necessity to perform the measurements of all polarization parameters with respect to different reference axes. In this context, new measurements could help to improve and set new constraints to the calculations. ALICE has measured the J/ψ polarization in pp collisions at √8= 7 TeV. The higher statistics of the 8 TeV data with respect to the 7 TeV data allows to extend the pT range of the measurements. This thesis presents a complete measurement of J/ψ polarization, i.e. the three polarization parameters, in two polarization frames : the Collins-Soper and Helicity frames. The results show no significant J/ψ polarization in the kinematic domain studied: 2.5 < y < 4.0 and 2 < pT < 15 GeV/c. The measurement of a frame invariant parameter λ, was also performed to ensure that no bias was present in the analysis procedure. The comparison with different theoretical predictions shows that there is not yet a satisfactory description of quarkonium production. None of the present theoretical approaches is able to describe both, the cross section and polarization measurements.

Quand un faisceau d'ions énergétiques traverse un plasma magnétisé plus rapidement que la vitesse d'Alfvén, il peut déstabiliser des ondes électromagnétiques. Cela résulte en une augmentation des fluctuations magnétiques, un ralentissement du faisceau et un chauffage du plasma. Cette instabilité faisceau-plasma magnétisée est commune dans des environnements comme les rayons cosmiques dans le milieu interstellaire ou les ions réfléchis au choc d'étrave terrestre, ou dans des plasmas de laboratoire. Sous certaines conditions, les collisions coulombiennes entre les ions peuvent avoir une influence et même supprimer le développement de l'instabilité. Ce travail fournit les premières recherches sur le sujet. Nous étudions l'instabilité numériquement avec un code hybride-PIC intégrant un module de collision Monte-Carlo nouvellement développé. Nos résultats pour le régime sans collision confirment les études précédentes sur la présence de modes résonants et non-résonant, et fournit une base de comparaison pour le cas collisionel. Nous trouvons que les collisions diminuent l'amplitude des fluctuations magnétiques, et identifions plusieurs régimes caractérisés par la compétition entre l'accroissement de l'instabilité et les collisions. Même dans des plasmas faiblement collisionnels, le ralentissement peut induire une augmentation rapide des échanges d'énergie collisionels, ce qui laisse moins d'énergie libre pour l'amplification des fluctuations magnétiques et cause un chauffage plus efficace du plasma. Pour le mode résonant, l'augmentation du chauffage du faisceau réduit le nombre de particules résonant avec les ondes et mène à une réduction de son taux d'accroissement. / When a beam of energetic ions streams in a magnetized plasma background with a bulk velocity higher than the local Alfvén speed, it can drive electromagnetic waves unstable. The result is enhanced magnetic field fluctuations, the slowing down of the beam and plasma heating. This so-called magnetic streaming instability is commonly present in space plasma, such as streaming cosmic rays in the interstellar medium or reflected ions at shocks, as well as in laboratory plasmas. Under certain physical conditions, Coulomb collisions between ions can influence and even suppress the development of the instability. This work provides the first investigation of such effects. We study the magnetic streaming instability numerically with a hybrid-PIC code with a newly developed Monte Carlo ion-ion Coulomb collision module. Our results for the collisionless regime confirm previous studies related to the existence of resonant and non-resonant modes, and provide the groundwork for the comparison with the collisional cases. We find that collisions generally lower the amplitude of the magnetic field fluctuations, and we identify several regimes which are characterized by the competition between the growth of the instability and collisions. Even in weakly-collisional plasmas, the slowing down of the beam can actually induce a rapid increase of collisional energy exchanges, which leave less free energy for the non-linear growth of the magnetic field fluctuations and cause a more efficient heating of the plasma. For the resonant mode the enhanced heating of the beam reduces the number of particles resonating with the waves and leads to a reduction of its growth rate.