Genetically modified (GM) crops are commercially cultivated worldwide but there are concerns on their possible negative impacts on soil biodiversity. A glasshouse study was conducted to determine effects of Bt maize residues on soil microbial diversity. Residues of Bt maize (PAN 6Q-308B) and non-Bt maize (PAN 6Q-121) were incorporated into the soil and corresponding maize seeds planted. The treatments were replicated three times. Fertilizer and water application were similar for both treatments. Rhizosphere and bulk soil was destructively sampled from each treatment and analyzed for microbial community level physiological profiles using Biolog plates with 31 different carbon substrates. Absorbance in the Biolog plates was recorded after 72 h of incubation at 20oC. Arbuscular mycorrhizal fungi spore counts were also determined. Field studies were conducted at the University of Free State and University of Fort Hare Research Farms to determine the effects of growing Bt maize on soil microbial diversity. One Bt maize cultivar (PAN6Q-308B) and non-Bt maize (PAN6Q-121) were grown in a paired experiment at University of Free State farm, while two Bt maize (DKC61-25B and PAN6Q-321B) and their near-isogenic non-Bt maize lines (DKC61-24 and PAN6777) were grown in a randomized complete block design with three replicates. Fertilization, weed control and water application, were similar for both Bt maize cultivars and their non-Bt maize counterparts. Rhizosphere soil samples were collected by uprooting whole plants and collecting the soil attached to the roots. The samples were analysed for microbial diversity and for arbuscular mycorrhizae fungal spore counts. Principal component analysis showed that soil microbial diversity was affected more by sampling time whereas genetic modification had minimal effects. Presence of residues also increased the diversity of microorganisms. Mycorrhizal fungal spores were not affected by the presence of Bt maize residues. Growing Bt maize had no effect on the soil microbial diversity in the rhizosphere.

MSc (Food Science and Technology) / Department of Food Science and Technology / White maize-based porridge is a staple food for about 80% consumers in South Africa and sub-Saharan African countries contributing significantly to the diet of rural populations in developing countries. White maize is deficient in some amino acids and over-dependency on its porridge may lead to high prevalence of malnutrition-related health conditions. Moringa oleifera (MO) and termite (Isoptera spp.) are known to contain a substantially high amount of protein. The aim of this study was to determine the effect of powders from MO leaves and termite on the nutritional and sensory properties of instant-maize porridge. Inclusion of MO and termite powders in instant-maize porridge, at different treatments, was considered using a completely randomised design. Factor levels were: AOB-control (maize flour); BEA (maize, powders of cooked dried MO and termite); CIA (maize, powders of blanched dried MO and termite) and DJE (maize, powders of uncooked dried MO and termite). Data were analysed using SPSS version 23. The protein content of fortified instant-maize porridge (FMP) significantly (p < 0.05) increased from 10.02 to 21.20% compared to unfortified porridges. The mineral content of FMP was higher in terms of Zn, Fe, Ca and Mg. Moisture content of FMP increased from 5.00 to 6.00%. Sensory analysis showed that among fortified porridges, CIA was rated high for colour and texture, BEA higher in taste and DJE higher for aroma. AOB had higher acceptance than fortified porridges for taste. Powder from MO leaves and termite could be used in complementary foods and food supplements to increase protein and mineral contents.

MSCAGR (Soil Science) / Department of Soil Science / Poultry manure (PM) is an inexpensive source of fertilizer but it decomposes quickly and releases carbon and greenhouse gases. Biochar (BC) could be an alternative source of carbon to improve soil quality and reduce greenhouse gas emission. This study investigated the effect of co-application of BC and PM on selected soil physical and chemical properties and performance of maize. A field experiment was conducted at the University of Venda experimental farm during 2015/2016 and 2016/2017 seasons. The experiment was a 4 x 3 factorial arrangement consisting of four rates of BC (0, 5, 10 and 20 t ha-1) and three rates of PM (0, 2, and 4 t ha-1) in a RCBD arrangement replicated three times. Maize was planted in both seasons. After harvest, soil bulk density was determined at four soil depths (0-5, 5-10, 10-15, and 15-20 cm), while aggregate stability and selected soil chemical properties were determined at two soil depths (0-15 cm and 15-30 cm). Data were subjected to ANOVA using Genstat 17th edition. The least significant difference was used to compare the treatment means at P < 0.05. Soil aggregate stability, organic carbon, Ca2+, Mg2+, K+, maize dry matter and maize grain yield increased with increasing rates of BC and PM application at 0 - 15 cm depth in both seasons. The combination of BC at 20 t ha-1 and PM at 4 t ha-1 significantly (P < 0.05) decreased soil bulk density at 5 - 10 cm depth but increased soil available P and total N at the two depths in both seasons. The results of this study suggested that BC and PM improved soil ability to retain and supply nutrients through improved soil aggregate stability and reduced bulk density thereby improving maize dry matter and grain yield. Combining BC with PM proved to enhance the ability of soil to function by improving selected soil physical and chemical properties thereby improving maize dry matter and grain yield. / NRF

MSCAGR (Plant Production) / Department of Plant Production / Smallholder farmers in Sub-Saharan Africa still obtain low grain yields in maize largely due to low soil fertility. The soils are inherently low in nitrogen (N) that is required for the proper development of the maize plant. Currently there are no commercial cultivars for low N tolerance locally. The combining ability approach can be used as a tool for breeding desirable cultivars. In order to improve grain yield in maize, it is important to consider ear prolificacy which is a major yield component. Therefore this study was designed to estimate combining ability in maize. Exotic germplasm from the International Maize and Wheat Improvement Center and the Institute of Tropical Agriculture as well as the local germplasm from the Agricultural Research Council was used in the study to generate crosses. One hundred and two crosses were evaluated together with a standard commercial check under low N and optimum N conditions. The specific objectives of the study were to determine general and specific combining ability for prolificacy among local and exotic inbred lines and evaluate the response of prolific hybrids to low N conditions. The hybrids were planted in the 2014/2015 summer season under irrigation in Potchefstroom, Cedara and Taung in field plots consisting of 0.75m x 0.25m spacing in a 0.1 alpha lattice design replicated twice. Data for agronomic attributes were recorded and subjected to analysis of variance using SAS version 9.1.3. Genetic correlations were analyzed using the Principal Components Analysis and factor analysis based on the correlation analysis and major traits. The results showed variation in agronomic performance among the inbred lines and their F1 hybrids. Inbred lines including TZEI63, T1162W, L15 and L17 showed positive GCA estimates for ear prolificacy at the different locations. Specific combining ability for prolific hybrids was positive at all locations and environments. The GCA:SCA ratio was close to unity; indicating that the number of ears per plant showed highly significant (P<0.01) correlation with grain yield. The hybrids showed ear prolificacy under the low N conditions. This trait can be used effectively in stress tolerance maize breeding programmes. / NRF

MSCAGR (Soil Sciece) / Department of Soil Science / In most parts of Limpopo Province of South Africa, crop yields are low and continue to decrease due to decline in soil fertility, which has been identified as a major constraint to crop production. Therefore, there is a pressing need for soil amendments such as the application of biochar, which has the potential to improve soil fertility due to its physical and chemical properties. Biochar is the product of incomplete combustion of biomass in the absence of oxygen. The overall objective of the study was to determine the effects of biochar and NPK fertilizer on maize performance and selected soil nutrient levels. A 3x2 factorial experiment was conducted at the School of Agriculture Experimental Farm for two consecutive seasons (2015/16 and 2016/17 seasons). Treatments consisted of biochar applied at three levels, viz. 0, 10 and 20 t/ha and NPK inorganic fertilizer applied at two rates viz. NPK0 (zero NPK fertilizer) and NPK1 {N (150 kg/ha) P (50 kg/ha) K (20 kg/ha)}. The treatments were laid out in a randomized complete block design (RCBD) and replicated three times. Maize cultivar (DKC 2147) was used as the test crop. Maize growth and yield measurements assessed included: plant height (cm), stem diameter (cm), number of leaves, leaf area, dry biomass (kg/ha), nutrient uptake, cob yield, grain yield and harvest index. Soil samples were collected from 0-10 cm and 10-20 cm soil depths at the end of each season to determine total N, P, K nutrient levels in the soil. Data collected was subjected to two-way analysis of variance using the general linear model (GLM) procedure of Genstat software version 17. Comparison of means was done using the Standard Error of Deviation (SED) method at 5% level of significance (p<0.05). Biochar and NPK fertilizer had no effect on total N and exchangeable K at all soil depths in 2015/16 and 2016/17 seasons. Biochar had no effect on phosphorus at all soil depths in 2015/16 and at 0-10 cm soil depth in 2016/17 season. The effect of biochar and NPK fertilizer was highly significant (p<0,001) on available P at 10-20 cm soil depth in 2016/17 season. Significant interactive effect of biochar and NPK fertilizer on soil total N at 10-20 cm (in 2015/16 season), available P and exchangeable K at 10-20 cm soil depth in 2016/17 season was also observed. Plant growth parameters increased with biochar addition at 20 t/ha and NPK1 (150 kg N/ha, 50 kg P/ha, 20 kg K/ha) fertilizer. The results of this study showed that biochar application at the rate of 10 and 20 t/ha has the potential to influence selected soil nutrient levels, maize growth, yield and yield components with and without NPK fertilizer application. Since this study was conducted over two seasons and biochar properties changes over a long-term period, more research is needed to evaluate the effect of biochar on soil nutrient levels and maize growth, nutrient uptake and yield over a long period of time. / NRF

Genetically modified (GM) white and yellow maize, Zea mays, has been commercially released and cultivated in South Africa since 1997/1998. The traits expressed are insect resistance and herbicide tolerance conferred by the bacteria Bacillus thuringiensis (Bt) Cry genes and Agrobacterium 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase gene, respectively. The Cry genes have been used widely to control lepidopteran insect pests but insect resistance to GM Bt crops has been a concern since the introduction of this technology. A management strategy includes refugia planting of 5% non-Bt plants, with no insecticide application, and 20%, where insecticide application is allowed. These refugia are designed to allow the survival of insect pests within restricted planted zones. However, in South Africa there are reports of Bt-resistant stem borer (Busseola fusca) (Fuller) (Lepidoptera: Noctuidae) and non-compliance with refuge planting. The aims of this study were two-fold: 1. To conduct a survey among KwaZulu-Natal (KZN) GM maize growers to ascertain information such as level of compliance with refuge planting and to determine which refugia were predominantly planted and reasons thereof; 2. To conduct a replicated field trial to determine yield, insect borer damage and economic benefit of the 5% unsprayed and 20% sprayed refuge options (including three configurations namely strip, perimeter and block and a 5 and 20% ‘refuge-in-a-bag’ option). The survey indicated that 28 out of 29 (96.6%) KZN Bt maize growers plant the 5% non-sprayed refuge with 27 (96.4%) of those respondents planting the strip configuration for the purpose of insect management (75%) and ease of planting (32.2%). The survey also showed that 7 (seven) i.e. 21.9% of KZN Bt maize growers observed borer damage and although growers are now fully compliant with refugia planting requirements, initially 7 respondents (24.1%) did not comply with or plant refugia correctly. Furthermore, 7 respondents reported insect borer damage in their maize with 4 of the 7 instances (57.1%) likely stemming from incorrectly planted refugia. vii No significant differences in yield or insect damage were observed between the 5 and 20% refugia for any of the planting configurations in the field trial. However due to costs involved with insecticide application and labour required for the operation in the 20% option, these treatments were less economically advantageous than the non-Bt control. The 20% block and strip configurations had a cost benefit ratio of ZAR 7.21 and ZAR 6.67 respectively, earned per R1 spent by the grower compared with ZAR 7.76 in the sprayed control. The cost-benefit comparison for the 5% block and strip configurations was ZAR 8.48 and ZAR 7.71, respectively compared with ZAR 9.44 in the unsprayed control. In addition, the 20% seed mixture limited borer damage to 4.95% when compared with 15.77% damage in the sprayed control (ANOVA, F pr = 0.124). The seed mixtures are not available commercially and the results from the survey indicated that some education and marketing by the seed companies would be advisable prior to their release to the farming community. In order to determine which of the refuge options between 5 and 20% would be more advantageous for growers overall, regardless of the planting configuration; data were grouped and analysed. There were no significant differences in either the yield or insect damage for the 5 and 20% refugia, but the cost-benefit calculations indicated that the 5% option was more cost effective – for the 5 and 20% refugia, ZAR 7.97 and ZAR 7.15 respectively, earned per ZAR 1 spent by the grower (ANOVA, F pr. = 0.03). This is because no insecticide was used in the 5% treatments. Mean ear damage comparisons between the 5 and 20% refugia showed that the 20% refuge in the perimeter configuration incurred the least damage (2.65% ear damage) compared with 5% perimeter (10.86% ear damage), although the reasons for this are not clear. While the results of the field trials showed no significant differences in insect damage and yield with regard to choice of refuge configuration, monitoring insect resistance management remains an integral part of Bt maize crops in South Africa, in order to delay further resistance development and to prolong the viability of Bt technology. / Agriculture and  Animal Health / M. Sc. (Agriculture)

The current interest in conservation agriculture (CA) technologies is a result of the need to reduce excessive land degradation in most crop producing areas as well as to enhance sustainable food production. Cover crops that are usually grown under CA to provide soil cover, may offer secondary benefits, depending on the farming system. The concept of growing cover crops is a relatively new phenomenon to smallholder farmers. Production of large biomass yields and weed suppression from cover crops were major challenges affecting success and uptake of CA technologies by smallholder irrigation farmers. Coupled with this, low soil fertility limit maize productivity and reduce water use efficiency on smallholder irrigation schemes in what is largely a water strained agro-ecology in South Africa. While cover cropping can increase maize productivity, benefits of different types of mulch are not well understood, leading to challenges in selecting the most appropriate cover crop species to grow in the Eastern Cape Province (EC) of South Africa (SA) which has a warm temperate climate. With respect to any new technology, smallholder farmers are more interested in the economic benefits. Cover crops have been defined as leguminous or non-leguminous plants used for ground cover in various temporal and special configurations used in crop or animal production systems. The purpose of these cover crops is to improve on or more of the following: soil erosion, availability and cycling of N, P, K, Ca and other nutrients, soil moisture and water infiltration, and weed or pest control (Eilitta et al., 2004).. Improvement of animal or human diet may be additional goals. This definition accommodates diverse systems which may include intercrop and sole-cropping systems. In the Eastern Cape Province of South Africa, a government initiative has promoted the growing of winter cover crops in smallholder irrigation schemes (Allwood, 2006). In other parts of Africa, legume food crops have been simultaneously grown with cereal staples to improve both soil cover and human diet (Eilitta et al., 2004). Winter experiments were undertaken in 2007 and 2008 to evaluate biomass accumulation, C and N uptake, weed suppression and response to fertilization. Winter cover crops planted included; oats (Avena sativa), grazing vetch (Vicia dasycarpa), faba bean (Vicia faba), forage peas (Pisum sativum) and lupin (Lupinus angustifolius). After cover crops were terminated, the effects of residues on weeds, fertility, moisture conservation and maize productivity were undertaken in the 2007/08 and 2008/09 summer seasons. Field studies were also done in the 2007/08 and 2008/09 summer seasons to investigate effects of strip intercropping patterns (3:2; 4:2; and 6:2 patterns) of maize (cv. PAN 6479) with mucuna (Mucuna pruriens) or sunnhemp (Crotalaria juncea) on maize productivity and summer cover crop biomass production. In a separate experiment effects of relay intercropping sunnhemp, mucuna and sorghum (Sorghum bicolor) on biomass accumulation and maize productivity were investigated. Decomposition, N and P release from both winter cover crops and summer cover crops were also assessed in laboratory incubation experiments. Oats, grazing vetch and forage peas cover crops produced mean dry mass of 13873 kg/ha, 8945.5 kg/ha and 11073 kg/ha, respectively, while lupin had the lowest dry mass of 1226 kg/ha over the two seasons. Oats responded to fertilization while, there was little or no response from the other winter cover crops. Oats and grazing vetch also reduced weed density by 90 % and 80 % respectively while lupin only reduced weed density by 23 % in relation to the control plots. Nitrogen uptake was 254 kg N/ha for oats while it was 346 kg N /ha for grazing vetch. In the subsequent summer season, grazing vetch and forage pea residues significantly (P < 0.01) improved soil inorganic N. Oat and grazing vetch residues significantly (P < 0.05) reduced weed dry masss and weed species diversity compared to plots with lupin residues and the control. Lack of maize fertilization tended to reduce maize yields but not for maize grown on grazing vetch residues. From an economic perspective, grazing vetch resulted in the highest returns. Decomposition of winter cover crops was much faster for grazing vetch followed by forage peas and lastly oats. Oats had 40 % ash free dry mass remaining after 124 days while grazing vetch and forage peas had 7 % and 16 % respectively. Maximum net mineralized N and P were greater for grazing vetch (84.8 mg N/kg; 3.6 mg P/kg) compared to forage peas (66.3 mg N/kg; 2.7 mg P/ha) and oats (13.7 mg N/kg; 2.8 mg P/kg). In the strip intercropping trials, sunnhemp achieved the highest biomass yield of 4576 kg/ha in the 3:2 pattern while mucuna achieved 1897 kg/ha for the same strip pattern. The 3:2 strip intercropping pattern slightly depressed yields, however, yield reduction was more pronounced in the first season where water stress was experienced. Growing maize on previous cover crop strips failed to increase maize productivity probably due to weed growth during the fallow reducing mineral N in these strips. Decomposition was faster in sunnhemp leaves and mucuna compared to sunnhemp stems. Sunnhemp stems had about 65 % of ash free dry mass remaining after the end of the experiment at 132 days while just over 10 % of mucuna and sunnhemp leaves still remained. Mucuna mineralized 60 mg N/kg and 3.2 mg P/kg and sunnhemp mineralized 45 mg N/kg and 3.5 mg P/kg. Relay intercropping did not significantly (P > 0.05) affect maize biomass and grain yield. Sorghum experienced the largest drop in biomass when relay-intercropped with maize. Mucuna resulted in the highest N uptake (271 kg N/ha) in sole cropping while sorghum had the lowest (88 kg N/ha). Grazing vetch results in high biomass yields with minimal fertilizer application in a warm-temperate climate. Grazing vetch mulch is also the most cost effective mulch for better early weed control, improving soil mineral N status, water conservation and ultimately enhanced maize productivity in smallholder irrigation maize-based systems. The 3:2 pattern maximizes summer cover crop biomass yields compared to the 6:2 and 4:2 patterns. However, the 3:2 pattern may slightly depress yields in a water stressed environment. Relay intercropping mucuna, sunnhemp and sorghum into a maize crop at 42 days after maize sowing has no effect on maize productivity while cover crop biomass yields are low. Having a long winter fallow period after maize harvesting, a common practice in the study area, reduces the positive impact of legume cover crops on soil mineral N. Results suggest that winter cover crops may result in weed control, soil fertility and maize yield improvement benefits while a long fallow period may cancel-out these benefits for summer cover crops. Grazing vetch is a cost effective cover crop that produces high maize yields with minimal fertilizer input. Maize growing on oat mulch requires more fertilizer application than crops growing on grazing vetch mulch. Conservation agriculture systems in which summer cover crops are grown alongside the maize crop with a long winter fallow period do not produce the intended CA benefits.

Genetically modified (GM) white and yellow maize, Zea mays, has been commercially released and cultivated in South Africa since 1997/1998. The traits expressed are insect resistance and herbicide tolerance conferred by the bacteria Bacillus thuringiensis (Bt) Cry genes and Agrobacterium 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase gene, respectively. The Cry genes have been used widely to control lepidopteran insect pests but insect resistance to GM Bt crops has been a concern since the introduction of this technology. A management strategy includes refugia planting of 5% non-Bt plants, with no insecticide application, and 20%, where insecticide application is allowed. These refugia are designed to allow the survival of insect pests within restricted planted zones. However, in South Africa there are reports of Bt-resistant stem borer (Busseola fusca) (Fuller) (Lepidoptera: Noctuidae) and non-compliance with refuge planting. The aims of this study were two-fold: 1. To conduct a survey among KwaZulu-Natal (KZN) GM maize growers to ascertain information such as level of compliance with refuge planting and to determine which refugia were predominantly planted and reasons thereof; 2. To conduct a replicated field trial to determine yield, insect borer damage and economic benefit of the 5% unsprayed and 20% sprayed refuge options (including three configurations namely strip, perimeter and block and a 5 and 20% ‘refuge-in-a-bag’ option). The survey indicated that 28 out of 29 (96.6%) KZN Bt maize growers plant the 5% non-sprayed refuge with 27 (96.4%) of those respondents planting the strip configuration for the purpose of insect management (75%) and ease of planting (32.2%). The survey also showed that 7 (seven) i.e. 21.9% of KZN Bt maize growers observed borer damage and although growers are now fully compliant with refugia planting requirements, initially 7 respondents (24.1%) did not comply with or plant refugia correctly. Furthermore, 7 respondents reported insect borer damage in their maize with 4 of the 7 instances (57.1%) likely stemming from incorrectly planted refugia. vii No significant differences in yield or insect damage were observed between the 5 and 20% refugia for any of the planting configurations in the field trial. However due to costs involved with insecticide application and labour required for the operation in the 20% option, these treatments were less economically advantageous than the non-Bt control. The 20% block and strip configurations had a cost benefit ratio of ZAR 7.21 and ZAR 6.67 respectively, earned per R1 spent by the grower compared with ZAR 7.76 in the sprayed control. The cost-benefit comparison for the 5% block and strip configurations was ZAR 8.48 and ZAR 7.71, respectively compared with ZAR 9.44 in the unsprayed control. In addition, the 20% seed mixture limited borer damage to 4.95% when compared with 15.77% damage in the sprayed control (ANOVA, F pr = 0.124). The seed mixtures are not available commercially and the results from the survey indicated that some education and marketing by the seed companies would be advisable prior to their release to the farming community. In order to determine which of the refuge options between 5 and 20% would be more advantageous for growers overall, regardless of the planting configuration; data were grouped and analysed. There were no significant differences in either the yield or insect damage for the 5 and 20% refugia, but the cost-benefit calculations indicated that the 5% option was more cost effective – for the 5 and 20% refugia, ZAR 7.97 and ZAR 7.15 respectively, earned per ZAR 1 spent by the grower (ANOVA, F pr. = 0.03). This is because no insecticide was used in the 5% treatments. Mean ear damage comparisons between the 5 and 20% refugia showed that the 20% refuge in the perimeter configuration incurred the least damage (2.65% ear damage) compared with 5% perimeter (10.86% ear damage), although the reasons for this are not clear. While the results of the field trials showed no significant differences in insect damage and yield with regard to choice of refuge configuration, monitoring insect resistance management remains an integral part of Bt maize crops in South Africa, in order to delay further resistance development and to prolong the viability of Bt technology. / Agriculture and  Animal Health / M. Sc. (Agriculture)