Thesis (M.Sc. (Agriculture)) --University of Limpopo, 2010 / Two studies were carried out to investigate the effect of cowpea cultivar supplementation on productivity of Pedi goats and Doper sheep fed ad libitum buffalo grass. Experiment 1 investigated the effect of cowpea cultivars supplementation on productivity of Pedi goats fed ad libitum buffalo grass hay using twelve goats that were assigned in a completely randomized design. The Pedi goats were fed ad libitum a basal diet of buffalo grass supplemented with four levels each of four cowpea cultivars namely, Pan 311, Red caloona, Black eye and Agripes. The four levels fed were 50, 100, 150 and 200 g/day. The experiment involved a 25 day preliminary period and a five day collection period during which feed intake, digestibility, live weight changes and nitrogen were measured. Based on available data, all the cowpea cultivars contained more than 15 % crude protein and can therefore be used as protein supplements to goats on low quality roughage. Pan 311 had higher feeding value (p<0.05), although it contained the highest concentration of condensed tannins. The concentration of tannins in Pan 311 did not exert negative effects on intake and digestibility. The data on a in vitro enzymatic digestibility of the cowpea cultivars and buffalo grass hay demonstrated that the cowpea cultivars had higher in vitro DM, OM and protein digestibilities that ranged from 0.64 to 0.75. The cowpea cultivars have high in vitro digestibility values thus implicating their suitability as supplements. Also chemical contents of the cowpea cultivars and the buffalo grass hay had poor capacity to predict forage in vitro digestibility. Forage intake and growth rate of Pedi goats were poorly predicted from in vitro digestibility. Experiment 11 investigated the intake and relative palatability indices of four cowpea cultivars offered to Pedi goats and Dorper sheep fed low quality buffalo grass hay. The experiment was a 2 (animal species goats and sheep) x 5 diets (four cowpea cultivars and buffalo grass hay) factorial arrangement in a completely randomized design. Intake and palatability of the goats and sheep were higher in Pan 311. Sheep had higher voluntary feed intake values than goats, but palatability indices were higher in goats than in sheep. Goats were better than sheep in the palatability indices ranking of the four cowpea cultivars. This seems to demonstrate that goats are better suited for assessing palatability indices ranking of cowpea hays and other similar legumes. Forage intakes and palatability indices ranking were poorly predicted from their nutrient. Growth of the goats was well predicted by forage in vivo digestibility and palatability indices. Also, forage intakes accurately predicted the palatability indices of the cowpea cultivars by the goats and sheep.

Characterization studies on the genetic diversity among cultivated cowpea (Vigna unguiculata (L.) varieties are valuable tools to optimize the use of available genetic resources by farmers, local communities, researchers and breeders. Eight cowpea (Vigna unguiculata (L.) genotypes ( Vegetable cowpea, Ivory grey, Okhalweni, Fahari, Fahari dark, 97K-1069-8, IT93K-73h, and 129-3) were subjected to molecular, morphological and agronomical characterization. DNA amplification fingerprinting markers were used to evaluate the genetic diversity among the eight genotypes. Nine random arbitrary primers were used to screen the eight genotypes to assess their ability to reveal polymorphisms in cowpea, and seven of them were selected for use in characterizing the total sample. A total of 43 bands were generated which are all polymorphic. On the average, the primers generated a total of 6.1 polymorphic bands. The resulting data-matrix included 43 analysed bands with a total of 344 characters. Neighbour joining analysis was used to generate the dendrogram, clustering the genotypes into two groups at an agglomerate coefficient of 0.30 irrespective of their geographical origins. The results also showed the presence of significant differences in morphological and quality traits among the genotypes. Fahari yielded the highest concentration of crude protein (46.51 mg/mg dry leaf) while Vegetable cowpea yielded the lowest (24.41 mg/mg dry leaf). The influence of manure was also found to be effective by increasing the crude protein content of the genotypes as shown by Fahari dark with an average of 53.53 mg/mg dry leaf as opposed to 39.85 mg/mg dry leaf without manure application. Although some small clusters grouped accessions of the same growth habits, a general lack of agreement between clustering and morphological features was observed. It can therefore be concluded that the significant differences between the molecular genetic analysis using DAF-PCR markers, morphologic characters and yield traits can be important tools to identify and discriminates the different cowpea genotypes.

Thesis (M.Sc. (Agriculture)) --University of the North, 2001

Thesis (M.Sc. (Horticulture)) --University of Limpopo, 2009 / Field experiments were conducted at the University of Limpopo experimental farm, Syferkuil during 2005/06 and 2006/07 production seasons. This was initiated to examine the effect of leaf removal on cowpea biomass, protein content and grain yield under sole and binary cultures. Treatments consisted of cowpea varieties (Pan 311 and Red caloona), cropping systems (sole and intercropping) and cowpea-leaf pruning regimes (pruning and un-pruned). Sweet corn was planted, as a component crop in the intercropped plots while sole sweet corn plot was included as a treatment. All treatment combinations were laid out as Randomize complete block design (RCBD) with four replicates. Supplementary irrigation was carried out during the plant growth period. Fully expanded leaves were harvested once on all cowpea plants in the two middle rows from designated plots at seven weeks after planting for each year. Growth and yield data were collected from component crops during the course of the trial while the protein content of harvested leaves and immature pods as well as the different cowpea plant parts at harvest were determined. Results of the study revealed that leaves of cowpea variety, Pan 311 harvested prior to the reproductive stage had significantly higher protein content than those of Red caloona. Protein content of immature Pan 311 pods had higher (18.8 to 25.1%) than Red caloona (17.9 to 20.7%) during both planting seasons. The percent protein content of cowpea stem obtained at harvest for Pan 311 varied between 9.3 and 9.4%, and between 9.9 and 12.3% for Red caloona during both planting seasons. Grain yield obtained for Pan 311 and Red caloona were 1703.7 kg ha-1 and 1479.8 kg ha-1, respectively during 2005/06 and 1290.7 kg ha-1 and 511.7 kg ha-1 respectively during 2006/07 planting seasons. Sweet corn intercropped with Red caloona during both planting seasons had higher average grain yield than when intercropped with Pan 311. Although intercropping decreased the partial land equivalent ratio (LER) value of individual component crops, the combined LER values of between 1.1 and 2.3 under intercrop for the different treatment combinations implies that the practice is advantageous. The results of post harvest soil analyses revealed that topsoil has the pH value of 7.11-7.29 indicating neutral soil while subsoil pH value of 6.27-6.91 indicated slightly acidic to neutral soil during both planting seasons. Based on the findings of this study, cowpea variety Pan 311 can be recommended as a better vegetable crop than Red caloona since it has higher leaf and immature pod protein content. It also had higher grain yield than Red caloona when intercropped with sweet corn. Sweet corn had high grain yield when intercropped with Red caloona than when intercropped with Pan 311. Keywords: Cropping systems, protein content, grain yields, leaf pruning and cowpea.

MSCAGR (Plant Production) / Department of Plant Production

Thesis (M.Sc. Agriculture (Agricultural Economics)) -- University of Limpopo, 2019 / Agriculture plays a significant role also serves as a critical economic sector in Limpopo province in terms of its contribution to the economy, and the number of employment opportunities it produces within local communities. The majority of people involved in agricultural practices are emerging farmers and smallholder/small scale farmers. These farmers try to earn a living from the production of livestock, broilers, fruits & vegetables, and cereals. Cowpea is a drought-tolerant legume that also serves as a staple food for the majority of Africans alongside maize and other typical staple foods consumed by most Africans. The crop is regarded as a key protein source for urban and rural poor, and plays an important role as a cash crop. Despite several nutritional benefits, economic importance and welfare enhancing potential of cowpea, farmers still do not have sufficient information on knowledge about the value that can be added to their cowpea production also the potential and competitiveness of this traditional leafy vegetable. The aim of the study was to map the value chain and determine the marketing efficiency of smallholder cowpea farmers in Capricorn and Waterberg districts of Limpopo province. The specific objectives were to: identify and describe socioeconomic characteristics of smallholder cowpea farmers in Capricorn and Waterberg districts of Limpopo province, identify and define the participants along the cowpea value chain and identify marketing constraints among smallholder cowpea farmers. Structured questionnaire was used to collect data from 80 smallholder cowpea farmers in Ga-Molepo of Capricorn district and Bela-Bela of Waterberg district using purposive sampling technique. Value chain mapping, descriptive statistics and binary logistic regression model were used in addressing the objectives. In identifying and defining the participants along the cowpea value chain, a value chain map was constructed to show the different stages cowpea goes through before reaching the final consumer. Two null hypotheses were formulated. The first hypothesis that stated smallholder cowpea farmers were inefficient in marketing cowpea was rejected. Marketing efficiency measure used to determine each farmer’s marketing efficiency revealed that 66% of smallholder cowpea farmers were efficient. The determinants of marketing efficiency were examined using binary logistic regression model. The second hypothesis stated that socioeconomic characteristics of smallholder cowpea farmers have no effect on the marketing efficiency. The v hypothesis was also rejected based on binary logistic results that revealed that age, household size, years in schooling, years in farming cowpea, income generated from selling cowpea, quantities of cowpea sold and occupation of the farmers were found to be significant in determining marketing efficiency of smallholder cowpea farmers. Pests, lack of access to formal markets and lack of information on how to process cowpea were major constraints farmers were faced with. It was recommended that value chain analysts, policy makers and extension workers together with other stakeholders assist in ensuring that food value chain relationships are established so that market opportunities can be created for smallholder cowpea farmers. In addition, farmer schools need to be introduced in rural areas were agricultural practices are dominant. At these schools, farmers can be taught about basic knowledge relating to agricultural production. Training should also be given to farmers on adopting technology as it can potentially assist in making production more efficient. / Water Research Commission (WRC)

Thesis (MSc. Agriculture (Agronomy)) -- University of Limpopo, 2019 / The traditional practice of farmers in Limpopo Province is to mixed and broadcast crops at planting without definite row arrangement. This practice hinders farm input application and results in low crop yields. Strip intercropping, where crops are planted with definite row arrangement, has the advantage of reducing inter-species competition, optimise plant population and increasing crop yield. This study aimed at improving cowpea-maize cropping systems using strip intercropping. The experiment was conducted at University of Limpopo farm and Ga-Thaba village. Five cowpea varieties (Glenda (check)), IT86K-499-35, IT82E-16, IT86D -1010, TVu-13464 and maize (PAN 6479) were evaluated using randomised complete block design with three replications. Data collected were days to flowering, days to maturity, plant height, canopy width, peduncle length, pod length, number of pods/plant, 100 seed weight, grain yield, fodder weight and land equivalent ratio was also determined. Data were analysed using the Statistix 9.0. The results revealed that in both locations TVu 13464 flowered early (50 days) respectively. At University of Limpopo farm TVu 13464, IT82E-16 and IT86D-1010 matured early (89, 88 and 91 days). At University of Limpopo farm, IT82E-16 had high cowpea grain yield (2230 kg/ha) under monocropping and also produced high grain yield of 1373 kg/ha during 2016/17 season. At Ga-Thaba, IT86D-1010 produced high cowpea grain yield of 1085 kg/ha under monocropping and during 2015/16 (660 kg/ha) while IT86K-499-35 also produced high grain yield of 915 kg/ha during 2016/17. The varieties showed yield stability depending on different locations. At University of Limpopo farm, strip intercropping achieved high maize grain yield of 3961 kg/ha during 2016/17. At Ga-Thaba, strip intercropping produced high maize grain yield 747 and 1024 kg/ha during 2015/16 and 2016/17, respectively. Monocropping produced low maize grain yield during 2015/16 with a mean of 425 kg/ha and mixed intercropping had mean of 499 kg/ha during 2016/17. The calculated LER for two crops over two seasons under strip intercropping ranged from 1.25 and 2.14, whereas under mixed intercropping, it ranged between 0.73 and 1.05 over two seasons at University of Limpopo farm. TVu 13464, IT82E-16 and IT86D-1010 are promising varieties for strip intercropping in low rainfall areas because of their early maturity and high grain yield. The calculated LER for two crops over two seasons xx under strip intercropping ranged from 1.62 and 2.98, whereas under mixed intercropping, it ranged between 0.76 and 1.67 in both seasons at Ga-Thaba. / National Research Foundation (NRF) and Water Research Commission (WRC)

Thesis (M.Sc. Agriculture (Agronomy)) -- University of Limpopo, 2019 / Limpopo Province is a semi-arid region prone to drought. Crop yields continue to decline due to low soil fertility and poor cropping systems. Cowpea is nutritionally rich in proteins essential for human consumption and livestock feeding. It fixes N2 which becomes available for the succeeding crop in rotation. For this reason, it is used as a companion crop in cereal-legume intercropping systems. Maize is one of the most important grain crops in South Africa, it serves as the major staple food for many households. Phosphorus is one of the macro-nutrient elements required by crops to produce satisfactory yields. The interactions between different rates of P fertilisation and cowpea-maize strip intercropping have not been studied in detail under rain-fed maize-cowpea strip intercropping in Limpopo Province. Many smallholder farmers in Limpopo Province obtain low yields due to the practice of mixed intercropping. Two season (2014/15 and 2015/16) experiments were laid out in a split-split plot design at Syferkuil farm to determine the performance of cowpea and maize varieties in cowpeamaize strip intercropping at varying P application rates. Treatments consisted of factors namely, P levels (0, 15, 30, 45 kg/ha), cropping system (monocropping and intercropping), maize varieties (WE3127 and ZM1423) and cowpea varieties (PAN311, TVu13464, IT86D-1010 and IT82D-889). Data were collected from growth and yield parameters that included (number of days to flowering, plant height, number of days to physiological maturity, root weight, number of pods per plant, unshelled net pod weight, number of cobs per plant, unshelled net cob weight and grain yield) in order to determine their performance. Results obtained revealed that P application levels significantly influenced most of the measured growth and yield parameters of both crops. PAN311 flowered earliest (49 days) across P levels. Increasing P application hastened the maturity of the varieties of PAN 311 and TVu13464 in both seasons. The P levels of 30 and 45 kg/ha reduced the number of days to maturity as compared to 0 and 15 kg/ha. TVu13464 variety produced more pods per plant (30) than other varieties. PAN311 yielded more grains (2491 kg/ha) than other varieties. Maize varieties performed well between P applications of 30 and 45 kg/ha. WE3127 yielded 3462 kg/ha whereas ZM1423 yielded 3306 kg/ha. Intercropping vi system performed better than monocropping system based on the measured growth and yield parameters. Two promising cowpea varieties (PAN311 and TVu13464) performed well and were selected based on their early maturity, drought tolerance and high yielding. Increasing P application levels increased crop yield. Optimum P levels for cowpea-maize strip intercropping were between 30 and 45 kg/ha. The calculated LER values were greater than one which indicates that intercropping was advantageous in land utilisation. The study showed the importance of P application in improving cowpea yield in cowpeamaize strip intercropping. / National Research Foundation (NRF) and University of Missouri, USA

Thesis (M.Sc. Agriculture (Agronomy) -- University of Limpopo, 2019 / Erratic rainfall and insect infestation are some of the factors that limit cowpea production in Limpopo (Asiwe, 2009). Improved cowpea varieties available for dryland production in Limpopo do not meet the farmer’s needs. Specific trait crosses are needed to be made with adapted varieties for the purpose of developing high yielding, pest tolerant and adapted varieties. Varieties with desired specific traits were planted in isolation plots for outcrossing by insects. F1 lines derived from parental lines that were half sib seeds were harvested and planted as F2, and seeds obtained from F2 generation were used to develop F3 segregating population at University of Limpopo Experiential Farm, Syferkuil, and planted along with parents. This F3 generation forms the current evaluation on which this report is based. Data collected included; aphid severity (measured on a 9-point scale), number of days to 90 % maturity, grain yield, 100 seed weight and number of genotypes selected for advancement. Results showed significant variation among progeny over parents therefore expressing transgressive segregation. Genotypes derived from pedigree TX08-30-5 were five while pedigree IT98K-205-8 and IT97K-499-35 had four genotypes each that expressed early maturity (80-90 days) which are desirable for evading drought. Twenty-three genotypes from pedigree GEC and three from pedigree IT97K-499-35 obtained grain yield (>101 g/plot and >161 g/plot) that was better than their parents. Only one genotype derived from pedigree TX08-30-1 expressed high resistance to aphids better than the parent with a mean aphid score range of 1-2. Four genotypes from pedigree TX08-30-5 obtained bigger 100 seed weight (>18.6 g) compared to parent. These progeny also proved to be high yielders as they expressed heterosis for all yield attributes than their parents. Eighty-two genotypes were selected for advancement. Significant variations were observed among progeny giving opportunity to make selections. In most cases, genotypes outperformed their parents which indicate positive heterosis. The 82 promising genotypes selected will be advanced for further selections from multi location testing for stability and adaptation.

Cowpea (Vigna unguiculata) and moringa (Moringa oleifera) are nutritious and medicinal vegetables, but could also harbor harmful microbial contaminants. The main aim of the project was to determine the effect of each processing techniques on the microbiology, proximate nutrients and shelf life of these vegetables to produce nutritious, tasty, safe and long lasting vegetable products. The processing techniques used were: washing, blanching, and drying. Leaf samples were collected at each stage of processing and were analysed for total viable count, coliform count, yeast and mould count and nutritional content. Microbial plate analysis showed the presence, particularly on cowpea leaves, of yeasts and bacteria such as Pseudomonas, Klebsiella, Staphylococci, Streptococci, and enterobacter including enteropathogens such as Salmonella spp., Shigella dysenteriae and E coli. The presence of E.coli on the leaves was also confirmed using polymerase chain reaction-amplified ribosomal DNA analysis. The most effective processing technique which reduced microbial load to below SABS standards while retaining nutritional quality was the washing of the leaves twice with tap water followed by steam tunnel blanching at 94oC for 12 minutes. Oven drying the leaves at 60oC gave satisfactory and extended shelf life results. Proximate analysis comparison of the two leaf types showed that on average moringa leaves contained more ash (2.37 vs 1.1 g), protein (6,9 vs 3,6 g), fat (0,41 vs 0.2 g) and energy (305,1 vs 70KJ) but less dietary fibre (0,9 vs 7,5 g) than cowpea leaves. No significant differences were noted in these values following washing and steam blanching. These results indicate that washing of these leaves is effective as to reducing microbial load and maintaining proximate values in the short term (up to 4 days) but that oven drying is effective for longer-term storage. / Life and Consumer Sciences / M. Sc. (Life Sciences)