The aim of this study was to investigate the strategies used to transfer aspects of culture in the translation of an English novel into Zulu. For this purpose, C.L. S. Nyembezi' s Zulu translation, Lafa Elihle Kakhulu ([1957] 1983), and Alan Paton's Cry, the Beloved Country ([ 1948] 1966) were used. In the study a cultural model for translation, used within the descriptive translation studies paradigm, was adopted in order to conduct a comparative analysis of proper names, terms of address, idiomatic expressions, figurative speech and aspects of contemporary life. It was found that Nyembezi mainly used cultural substitution, transference, domestication, addition and omission as translation strategies. The findings also showed that in resorting to these strategies certain rnicrotextual shifts resulted in macrotextual modifications of the translated novel as a whole. The macrotextual elements of the translated text most affected by microtextual shifts are characterisation and focalisation which, in turn, influence style and theme. / African Languages / M.A. (African Languages)

A cólica infantil, caracterizada por choro excessivo e inconsolável do bebê, é um problema comum no período inicial da vida, causando angústia e preocupação às mães. Apesar de ser uma manifestação comum, sua causa ainda é desconhecida. Assim, é necessário propor intervenções ao assistir as mães desses bebês, considerando as necessidades e dificuldades desta clientela no sentido de auxiliá-la a enfrentar e superar este problema. Este estudo teve como objetivo compreender a experiência materna de ter um filho com cólica no período precoce da vida. Optou-se por uma abordagem qualitativa, a Teoria Fundamentada nos Dados e como referencial teórico para análise e interpretação dos dados, o Interacionismo Simbólico. O método de análise de conteúdo baseou-se em 12 discursos de mães que tinham ou tiveram filhos que sofreram de cólica no período inicial da vida e que freqüentavam o seguimento de puericultura e pediatria de um Centro de Saúde Escola, situado na zona oeste da cidade de São Paulo. Os dados foram obtidos por meio de entrevista e observação do contexto, cuja análise permitiu compreender a experiência materna do fenômeno CONVIVENDO COM UM FILHO COM CÓLICA e a categoria central revelou-se como APRENDENDO A LIDAR COM A CÓLICA DO FILHO. O modelo teórico mostra que ter um filho com cólica é uma vivência angustiante e independe de ser mãe de primeiro filho. A compreensão da experiência materna de conviver com um filho acometido de cólica aponta para a necessidade dos profissionais reverem as práticas implementadas no manejo da cólica infantil. O acompanhamento do binômio mãe-filho por meio de atendimento de seguimento no mínimo até o terceiro mês de vida do bebê, incluindo atendimentos domiciliares e a promoção de estratégias de apoio implementadas pelos serviços de saúde podem contribuir para o estabelecimento saudável do vínculo mãe-filho. / The infantile colic, which is characterized by excessive and disconsolate baby’s cry, is a common problem during the initial period of life. It causes the mothers\' distress and worry. Although it is a common manifestation, its cause is still unknown. Then, considering these babies’ mothers’ needs and difficulties, it is necessary to propose an intervention while assisting them, in order to help them to face and overcome the problem. The aim of this study was to understand the experience of the mothers who had a baby with colic during the early period of life. The Theory Based on the Data was the qualitative approach used and the Symbolic Interaction was used as the theoretical reference to analyze and interpret the data. The method used to analyze the content was based on 12 speeches given by mothers who have or had their babies with colic during the initial period of life. These mothers attended the puerile and pediatric segment of a School Health Center located in the west zone of São Paulo city. The data were collected by interviews and observation of the context. The data analysis allowed the understanding of the maternal experience and the phenomenon is LIVING WITH A CHILD WHO HAS COLIC and the central category is LEARNING TO DEAL WITH THE CHILD’S COLIC. The theoretical model shows that having a baby with colic is a distressful experience and it does not matter if it is the mother’s first child or not. The understanding of the mother’s experience who has a child with colic points out that the professionals need to check the practices used while dealing with a child who has colic. The process of following the binomial mother-son by giving them assistance at least until the baby’s third month of life, including homecare and the promotion of supporting strategies implemented by the health services and contributing to the healthy establishment of the mother-child bond.

Dans les trois ouvrages étudiés Estrella Distante (1996), La pista de hielo (1993) et Amberes (2002) nous révélons la présence, parfois patente, parfois latente, du mythe de la malédiction. Notre étude rend compte d’une lecture à la lumière du mythe littéraire moderne de l’écrivain maudit. L’évolution de l’œuvre de Bolaño, montre que depuis le début le mythe de la malédiction lutte pour prendre une place centrale dans son œuvre.Un seul motif reste constant, inamovible, dans les trois romans en particulier et dans l’œuvre entière en général : l’héroïsme. Nous voyons dans cette valeur inaltérable du mythe de l’écrivain maudit chez Bolaño, le cœur de son éthique littéraire. L’héroïsme du poète maudit n’est pas associé à l’idée romantique d’une mission personnelle fondamentale mais plutôt à la valeur d’incorruptibilité. Les deux mythèmes PURETE-DETRESSE suscitent les deux motifs mythiques INGENUITE-MARGINALITE. Ceux deux motifs, filtrés par le temps et l’écriture conduisent à l’HEROISME et à l’INCORRUPTIBILITE, valeur qui définit le poète maudit dans l’ensemble de l’œuvre.La réécriture de ce mythe n’est pas sans conséquences dans l’univers de la réception. Bolaño se crée dans ses textes une image de lui-même qui prospère ensuite dans l’inconscient collectif ; son image devient l’archétype de l’écrivain latino-américain contemporain. La nouveauté de l’œuvre de Bolaño est d’avoir su placer la figure du poète maudit au cœur de son univers littéraire. Comme il est courant dans la reprise d’un mythe par un écrivain, Bolaño vise par sa réécriture un modèle à vocation universelle, sans que pour autant sa vie soit l’original ou la copie de ce modèle. L’image archétypale de l’homme Roberto Bolaño repose sur un univers symbolique de représentations qui ont un lien intime avec la malédiction.Bolaño insiste sur une autre forme de malheur, la rébellion, la révolte du maudit. Elle a été l’une des premières abordée dans la réception de son œuvre, et traduite comme une tendance antisystème. Bolaño est donc perçu comme un provocateur net, chose qui est absolument vraie. Cependant, c’est à travers la figure du maudit que la révolte littéraire s’effectue vraiment. En s’attaquant aux institutions littéraires, le poète renonce à l’appui institutionnel et déclare conquérir son indépendance à l’égard des pouvoirs littéraires. Pour chercher à être indépendant des pouvoirs littéraires il faut nécessairement en être d’abord dépendant ! Si le poète-type de Bolaño n’écrit presque pas, ou du moins pas comme il devrait le faire en tant qu’écrivain, de quelle sorte d’indépendance littéraire est-il question ? Cela pose un problème de fond celui de la vie comme œuvre (ce fut le cas pour le dandysme), et comme œuvre littéraire. Si la vie peut devenir littérature et si l’existence peut se changer en œuvre d’art, c’est à cette transformation que le poète bolanien se donne corps et âme. Faire de sa vie un objet littéraire est donc la tâche curieuse et très originale que s’impose le poète maudit chez Bolaño.En réécrivant le mythe du poète maudit, Bolaño a ouvert la voix à une nouvelle manière de voir la littérature et d’en faire. Ce mythe, bien connu en Europe depuis Villon jusqu’à Houellebecq, a été ravivé dans l’œuvre de Bolaño, en même temps qu’il a été introduit dans l’imaginaire de la littérature hispano-américaine, pour devenir aujourd’hui un symbole à double effet : consommation de masse d’une icône ou chemin offert au public vers le sens caché de l’expression. / Within the three books studied Estrella Distante (1996), La pista de hielo (1993) et Amberes (2002) we show the underlying- evident presence, of the literary malediction myth. We read Bolaño’s works lightened by the literary modern myth of the damned poet. The evolution of Bolaño’s work reflects, since the beginning the central space this modern myth takes into his imaginary. One of the unchangeable topics in the three novels studied is heroism. This inalterable value of his damned characters represents the central part Bolaño’s literary ethics. The damned poet heroism in this case is not associated to the romantic idea of a personal fundamental mission to be accomplished but rather to the moral value of incorruptibility. We can find two mythic topics PURITY-DISTRESS that produce the mythic patterns INGENUITY-MARGINALITY. The presence of this two patterns lead towards the two moral-ethic values that define Bolaño’s damned poet: HEROISM and INCORRUPTIBILITY.The process of rewriting this myth brings consequences in the reception’s world. Bolaño invents through his literature an image of himself which lives in the collective subconscious. This image is nothing different than archetype of what should be the contemporary Latin-American writer. As it is current in the process or rewriting of a myth, Bolaño aims a figure to universal vocation (archetype), but not with the purpose of making of his personal life the original and of his damned poet the copy. For the community of readers, the archetypal image of the man (Roberto Bolaño) rest upon a symbolic universe of representations directly connected with literary malediction.Bolaño insists on another way of curse; the rebellion of the damned poet. This feature has been translated in the context of the reception as an anti-system tendency. Bolaño is perceived as an agitator. Nevertheless, this feature of the writer comes from the literary rebellion of his characters. The damned poet of his work attacks the literary institutions renounces to the institutional support and declares the conquest of his independence in front of the literary powers.In order to become independent of the literary institutions, the poet must be necessarily dependent of them. As we know the character of the poet in Bolaño’s work do not really write or rather barely writes. So we ask what kind of literary independence is sought by the poet? This question introduce the idea of life a piece of art, (dandyism) and more exactly in this case; life as a literary piece of art. If life can become literature and existence can be transformed in a piece of art, Bolaño’s damned poet does not seek any more than fulfill this possibility of mutation. Make of his life a literary (ethic, moral and esthetic) object is the curious and original task the damned poet force himself on.Throughout the rewriting of the damned poet myth, Bolaño lights the way to an original way of perceiving but above all an original way of making literature. The myth of the damned poet, well know from Villon to Houellebecq, has been brighten up in Bolaño’s work and reintroduce in the Hispano-American literary imaginary. This version of the myth has become today a symbol that can be read in two ways: consummation product of an image or opened possibility of a secret meaning.

A life cycle of many known algae is influenced by the circadian clock. It seems probable that also Chromera velia, the closest known photosynthetic relative to Apicomplexa, uses circadian oscillator. We tested, if fluctuating of zoospore?s abundance is driven by the circadian clock. This rhythm is stable even in the constant light condition. Furthermore, three cryptochrome genes, involved in circadian rhythms, were sequenced and consequently phylogenetically investigated. Two of them were classified as CRY-DASH, the third is CRY-DASH-like. The rhythmicity of CRY-DASH expression in C. velia was also investigated. The level of expression was also tested under constant light and dark condition. It was suggested that expression of these genes is associated with the circadian oscillator. This is the first study of the circadian clock in C. velia.

The aim of this study was to investigate the strategies used to transfer aspects of culture in the translation of an English novel into Zulu. For this purpose, C.L. S. Nyembezi' s Zulu translation, Lafa Elihle Kakhulu ([1957] 1983), and Alan Paton's Cry, the Beloved Country ([ 1948] 1966) were used. In the study a cultural model for translation, used within the descriptive translation studies paradigm, was adopted in order to conduct a comparative analysis of proper names, terms of address, idiomatic expressions, figurative speech and aspects of contemporary life. It was found that Nyembezi mainly used cultural substitution, transference, domestication, addition and omission as translation strategies. The findings also showed that in resorting to these strategies certain rnicrotextual shifts resulted in macrotextual modifications of the translated novel as a whole. The macrotextual elements of the translated text most affected by microtextual shifts are characterisation and focalisation which, in turn, influence style and theme. / African Languages / M.A. (African Languages)

This thesis considers various roles of circadian clock genes in insect physiology. Application of molecular-biology methods in Pyrrhocoris apterus, non-model insect species, enable us to investigate involvement of circadian clock genes in photoperiod induced physiological responses. We discover involvement of neuroendocrine cells, and a role of Juvenile hormone (JH) signalization in transduction of photoperiodic signalization to peripheral tissues. We found new principles of JH signal diversification in tissue specific manner, and in addition described molecular mechanism of photoperiod induced changes in gut physiology. Comparison of gut and fat body tissue reveals that mechanism observed in the gut is tissue specific, and that circadian clock genes exhibit tissue specific functional pleiotropic effect.

Made available in DSpace on 2014-06-11T19:32:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-02-26Bitstream added on 2014-06-13T19:42:46Z : No. of bitstreams: 1 costa_jrv_dr_jabo.pdf: 396090 bytes, checksum: 23a92262b1f5d4ef9aee14c8383cae79 (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Aedes aegypti (Linnaeus), principal vetor da dengue no Brasil, tem sido combatido com o uso maciço de produtos químicos, contribuindo com o desenvolvimento de resistência e inviabilizando o controle do inseto. Bioinseticidas à base de Bacillus thuringiensis vêm apresentando resultados satisfatórios no controle de dípteros, devido à produção de proteínas bioinseticidas denominadas Cry (cristal), Cyt (citolíticas) e Chi (quitinase) e os efeitos sinergísticos existentes entre elas. O presente trabalho objetivou a seleção de isolados de B. thuringiensis, portadores de genes cry, cyt e chi com alta eficiência no controle de A. aegypti. Uma coleção de 1073 isolados de B. thuringiensis, provenientes de diversas regiões brasileiras, foi submetida à Reação em Cadeia da Polimerase (PCR), com base nos iniciadores específicos para os genes cry4Aa, cry4Ba, cry10Aa, cry11Aa, cry11Ba, cyt1Aa, cyt1Ab, cyt2Aa e chi. Os isolados dípteros-específico foram avaliados quanto à estruturação genética e através de bioensaios seletivos e quantitativos, para determinação da CL50 e CL90. Apenas 45 (4,19%) apresentaram amplificação para os genes cry e cyt e destes, 25 (54,34%) eram quitinolíticos. Foram definidos 21 haplótipos entre os 45 isolados de B. thuringiensis, os quais foram submetidos aos bioensaios seletivos, indicando 13 isolados que causaram 100% de mortalidade às larvas de A. aegypti. Os bioensaios quantitativos e análise de agrupamento permitiram selecionar quatro isolados altamente tóxicos às larvas de A. aegypti. A identificação dos genes cry, cyt e chi de B. thuringiensis e análise dos efeitos sinergísticos entre as toxinas, associadas à análise da toxicidade a insetos vetores permitiram a seleção de isolados que poderão ser utilizados em formulações de novos bioinseticidas brasileiros, podendo contornar possíveis problemas de resistência. / Aedes aegypti (Linnaeus), the main vector of dengue fever in Brazil, has been controlled with massive use of chemical products wich, contributed to the development of resistance decreasing the insect control efficacy. Bioinsecticides based on Bacillus thuringiensis is currently presenting satisfactory results on controlling dipterans, due to the production of Cry proteins (crystal proteins), Cyt (citolitic) and Chi (chitinase) with synergistic effects between them. The present work aimed to select B. thuringiensis isolates with cry, cyt and chi genes exhibiting high efficiency on A. aegypti larvae. A bacterial collection containing 1073 isolates of B. thuringiensis, obtained from different Brazilian locations, had their DNA isolated and submitted to PCR amplifications using specific primers for the genes cry4Aa, cry4Ba, cry10Aa, cry11Aa, cry11Ba, cyt1Aa, cyt1Ab, cyt2Aa e chi. The dipterans-especific isolates were then evaluated for the genetic structure and through selective and latter to quantitative bioassays for the determination of LC50 and LC90. Forty five out of 1073 isolates (4.19%) presented amplicons for the cry and cyt genes and from these 25 (54.34%) were detected as chitinolitic. Twenty one haplotypes were identified among the 45 isolates of B. thuringiensis, which were submitted to the selective bioassays, indicating 13 isolates that caused 100% mortality of A. aegypti larvae. The quantitative bioassays and the statistical analysis have pointed four toxic isolates to A. aegypti larvae. The identification of the cry, cyt and chi genes of B. thuringiensis and the analysis of the synergistic effects among the toxins, along with the toxicity analysis towards the vector insects allowed the selection of a set of isolates that can be used for the formulation of new bioinsecticides from Brazilian origin, that would avoid or at least delay the appearance of resistance problems.

Made available in DSpace on 2014-06-11T19:27:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-06-30Bitstream added on 2014-06-13T19:55:58Z : No. of bitstreams: 1 machado_tro_me_jabo.pdf: 255795 bytes, checksum: 94bed73df71cf07445d3e1f703d8704c (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Um total de 109 cepas de Staphylococcus coagulase-negativa foi isolado de mastite bovina clinica e subclinica em 35 fazendas de leite situadas em 9 estados brasileiros no período de fevereiro a maio de 2005, elas foram investigadas em relação a sua susceptibilidade in vitro a diversos agentes antimicrobianos. Entre as cepas obtidas, a resistência a penicilina foi a mais freqüente (93,5%), seguida pela resistência a sulfonamida (88,9%), novobiocina (88,6%) e ampicilina (85,3%). Todas as cepas examinadas mostraram resistência a pelo menos 1 das drogas antimicrobianas testadas. Cepas apresentando resistência múltipla foram extremamente comuns, com 10,0% dos isolados apresentando resistência a todas as drogas antimicrobianas. Os resultados obtidos indicaram que as cepas de Staphylococcus coagulase-negativas isoladas no Brasil apresentaram um alto grau de resistência a antimicrobianos. Estes resultados são provavelmente uma conseqüência da pressão devido ao uso intensivo de drogas antimicrobianas e eles são um motivo de preocupação em relação a saúde pública. / A total of 109 strains of coagulase-negative Staphy/ococci were isolated from bovine clinical and subdinical mastitic cattle in 35 dairy farms, in 9 states of Brazil from february to may 2005, and investigated for in vitro susceptibility to several antimicrobial agents. Among the isolates, resistance to penicillin was most frequent (93.5%), followed by resistance to sulphonamide (88.9%), novobiocin (88.6%) and ampicillin (85.3%). Ali isolates exhibited resistance to at least 1 of the antimicrobial drugs tested. Multidrug resistant isolates were quite common, 10.0% of the isolates showing resistance to ali antimicrobial drugs tested. This indicates that coagulase-negative Staphy/ococci in 3razil exhibit a high degree of antimicrobial agents' resistance, probably as a :onsequence of the pressure of the intensive use of antimicrobial drugs, a practice that :onstitute a public health concem.

Orientador: Manoel Victor Franco Lemos / Banca: Lúcia Maria Carareto Alves / Banca: Ricardo Antonio Polanczyk / Resumo: Bacillus thuringiensis durante sua fase de esporulação produz proteínas cristal, codificadas pelos genes cry, podendo atingir pragas de diversas ordens. Esta bactéria é utilizada no controle de pragas como a Spodoptera frugiperda, causadora de sérios prejuízos na agricultura. O objetivo desta pesquisa foi caracterizar os isolados MT6, MT7, SP8, SP6, PR2 e as linhagens H D73 e CRY-B de B. thuringiensis visando o controle de S. frugiperda. Foi utilizada a técnica de PCR que permitiu a identificação das subclasses do gene cry1 A (Aa, Ab, Ac e Ae). O RAPD determinou similaridade entre todos os isolados e linhagens e somente a subsp. CRY(-)B apresentou 50 % de similaridade em relação às demais. Evidenciaram-se dois perfis diferentes entre todos e a B ausência plasmidial na subsp. CRY(-). A resistência e susceptibilidade aos antibióticos estreptomicina, canamicina, rifampicina e eritromicina, nas concentrações de 40, 60, 80, 100 e 120 uglml, permitiu constatar que somente a linhagem CRY(-)B apresentou resistência para rifampicina. Todos os isolados e linhagens foram resistentes a canamicina e susceptíveis a eritromicina e para estreptomicina todos foram resistentes em concentração variadas. Nos bioensaios os isolados MT6, MT7, PR2, SP6, SP8 e a linhagem HD73, demonstraram potencial no controle de lagartas de primeiro instar de S. frugiperda. / Abstract: Bacillus thuringiensis is an entomopathogenic bacteria that during sporulation produces crystal proteins, coded by cry genes, able to act on a great number of organisms. Such bacteria is used to control agronomic pests such as Spodoptera frugiperda, the causal agent of serious problems on the agroindustry. The aim of the present work was to characterize the isolates MT6, MT7, SP8, SP6, PR2 and the strains of the bacterium B. thuringiensis subsp. kurstaki HO-73 e CRY(-)B aiming the control of S. frugiperda. The RAPO have determined 90% of similarity among the isolates and the strain CRY (-)B presented 50% similarity with the other ones. The plasmid profiles analyses has revealed similarities among the isolates SP6, SP8, MT6 and MT8 and differences between this strain HO-73 and isolate PR2. The MICs were conducted with the antibiotics streptomycin, kanamycin, rifampicin and erythromycin on the following concentrations 40, 60, 80, 100 and 120 ug/ml. Only the strain CRY(-)B has presented resistance to rifampicin. The bioassays results have shown that the MT6, MT7, SP8, SP6, PR2 isolates and the HO-73 exhibited 100 % efficiency for the biological control of S. frugiperda larvae. / Mestre

A cólica infantil, caracterizada por choro excessivo e inconsolável do bebê, é um problema comum no período inicial da vida, causando angústia e preocupação às mães. Apesar de ser uma manifestação comum, sua causa ainda é desconhecida. Assim, é necessário propor intervenções ao assistir as mães desses bebês, considerando as necessidades e dificuldades desta clientela no sentido de auxiliá-la a enfrentar e superar este problema. Este estudo teve como objetivo compreender a experiência materna de ter um filho com cólica no período precoce da vida. Optou-se por uma abordagem qualitativa, a Teoria Fundamentada nos Dados e como referencial teórico para análise e interpretação dos dados, o Interacionismo Simbólico. O método de análise de conteúdo baseou-se em 12 discursos de mães que tinham ou tiveram filhos que sofreram de cólica no período inicial da vida e que freqüentavam o seguimento de puericultura e pediatria de um Centro de Saúde Escola, situado na zona oeste da cidade de São Paulo. Os dados foram obtidos por meio de entrevista e observação do contexto, cuja análise permitiu compreender a experiência materna do fenômeno CONVIVENDO COM UM FILHO COM CÓLICA e a categoria central revelou-se como APRENDENDO A LIDAR COM A CÓLICA DO FILHO. O modelo teórico mostra que ter um filho com cólica é uma vivência angustiante e independe de ser mãe de primeiro filho. A compreensão da experiência materna de conviver com um filho acometido de cólica aponta para a necessidade dos profissionais reverem as práticas implementadas no manejo da cólica infantil. O acompanhamento do binômio mãe-filho por meio de atendimento de seguimento no mínimo até o terceiro mês de vida do bebê, incluindo atendimentos domiciliares e a promoção de estratégias de apoio implementadas pelos serviços de saúde podem contribuir para o estabelecimento saudável do vínculo mãe-filho. / The infantile colic, which is characterized by excessive and disconsolate baby’s cry, is a common problem during the initial period of life. It causes the mothers\' distress and worry. Although it is a common manifestation, its cause is still unknown. Then, considering these babies’ mothers’ needs and difficulties, it is necessary to propose an intervention while assisting them, in order to help them to face and overcome the problem. The aim of this study was to understand the experience of the mothers who had a baby with colic during the early period of life. The Theory Based on the Data was the qualitative approach used and the Symbolic Interaction was used as the theoretical reference to analyze and interpret the data. The method used to analyze the content was based on 12 speeches given by mothers who have or had their babies with colic during the initial period of life. These mothers attended the puerile and pediatric segment of a School Health Center located in the west zone of São Paulo city. The data were collected by interviews and observation of the context. The data analysis allowed the understanding of the maternal experience and the phenomenon is LIVING WITH A CHILD WHO HAS COLIC and the central category is LEARNING TO DEAL WITH THE CHILD’S COLIC. The theoretical model shows that having a baby with colic is a distressful experience and it does not matter if it is the mother’s first child or not. The understanding of the mother’s experience who has a child with colic points out that the professionals need to check the practices used while dealing with a child who has colic. The process of following the binomial mother-son by giving them assistance at least until the baby’s third month of life, including homecare and the promotion of supporting strategies implemented by the health services and contributing to the healthy establishment of the mother-child bond.