Submitted by Fabio Sobreira Campos da Costa (fabio.sobreira@ufpe.br) on 2016-09-21T12:18:22Z No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) TESE Cesar Costa Junior.pdf: 3090772 bytes, checksum: b18f3c21a3f1df343da0d3d471aba453 (MD5) / Made available in DSpace on 2016-09-21T12:18:22Z (GMT). No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) TESE Cesar Costa Junior.pdf: 3090772 bytes, checksum: b18f3c21a3f1df343da0d3d471aba453 (MD5) Previous issue date: 2016-03-11 / FACEPE / O status taxonômico de espécie tem se mostrado essencial em relação a conservação de espécies como também para estudos eco-epidemiológicos. O status taxonômico em insetos vetores tem demonstrado que muitas espécies anteriormente ditas como únicas, compreendiam, na verdade, um complexo de espécies crípticas em que seus integrantes podem ter capacidades vetoriais diferenciadas, e.g. Anopheles gambie Lutzomyia longipalpis sl, inseto vetor da Leishmania infantum (agente etiológico da leishmaniose visceral americana), possui status taxonômico ainda controverso e diversos estudos têm sido realizados à fim de esclarecer o real status deste vetor. Apesar de diversas populações apresentarem características exclusivas (e.g. feromônios, sons copulatórios, padrões de manchas abdominais e estrutura genética), a quase duas décadas apenas uma espécie do complexo foi descrita, a L. pseudolongipalpis. Foi avaliado três localidades do nordeste do Brasil, das quais duas possuem como barreira geográfica a Chapada do Araripe. Foram utilizadas metodologias robustas como Análise de Máxima Verossimilhança, Teste de atribuição e AMOVA.Este estudo utilizou um fragmento do gene period, sendo que o Teste de Atribuição, Fst e a Máxima Verossimilhança separaram agruparam os dados genéticos com as diferenças fenotípicas e pela primeira vez o AMOVA demonstrou que a maior variação genética estava na relação entre o número de manchas abdominais e a estrutura genética das populações, sendo o primeiro trabalho a evidenciar a ancestralidade do fenótipo que apresentava uma mancha abdominal (1S) em relação ao fenótipo com duas manchas (2S). O Fst encontrado evidenciou, pela primeira vez, as relações filogeográficas separando moderadamente as populações isoladas pela a chapada do Araripe. / he taxonomic status of species is essential for conservation and has also been of great importance in eco-epidemiological studies. The definition of the taxonomic status for insect vectors has demonstrated that several previously identified species are in fact comprised of a complex of cryptic species with different vectorial capacity (e.g. Anopheles gambie). For the sand fly Lutzomyia longipalpis sl, principal vector of Leishmania infantum, the etiological agent of American visceral leishmaniasis, the taxonomic status remains controversial and several studies have been conducted to clarify the actual status of this vector. Although diverse populations present unique characteristics (e.g. pheromones, copulatory sounds, patterns of abdominal spots and genetic structure), for nearly two decades only one species of the complex was described, L. pseudolongipalpis. In this study, we evaluated three sites in Northeastern Brazil where two of them have as geographical barrier the plateau of Araripe. The study used robust methods such as Maximum Likelihood analysis, assigning test and AMOVA. The present study used a fragment of the period gene (525 base pairs), and has demonstrated for the first time a relationship between the number of abdominal spots and the genetic structure of the population, being first study to show the ancestry of the phenotype showed one abdominal spot (1S) compared to the phenotype with two spots (2S). As well as the Fst found separated populations isolated by the Araripe plateau.

The subfamily Phryninae (Arachnida: Amblypygi) has not been recently revised, which causes many difficulties for species identification. Thus far, no phylogenetic hypothesis has been proposed for this subfamily. The nomenclature of diagnostic characters is not uniform, and most illustrations are poorly detailed. We reviewed the subfamily Phryninae, redescribed all known species, and described six new species. We proposed a unified nomenclature for teeth of chelicerae and pedipalpal spines. We performed a phylogenetic analysis using total evidence and direct optimization in the program POY. We built a morphological matrix of 92 terminals and 174 characters, and a molecular matrix with 1557 pb (markers COl, 12s and 16s). Both sets of information were analyzed separately to understand their influence over the total evidence analysis. The results of the three analyses were different: the morphological analysis did not recover the subfamily Phryninae as monophyletic, this analysis produced 90 equally parsimonious topologies, however, the strict concensus tree had good resolution. The molecular analysis did not recover the family Phrynidae as monophyletic, but Phryninae was recovered. Total evidence analysis allowed for obtain just one more parsimonious hypothesis which included all species of Phrynidae, and resolved the politomies obtained with the analysis using morphology only, in this hypothesis Phrynidae and its subfamilies are monophyletic. ln all results, the genera of Phryninae were polyphyletic. We selected the tree of total evidence analysis to build a new taxonomic proposal, we decided to keep Acanthophrynus, Phrynus, and Paraphrynus and to create five new genera: Caicedophrynus gen. nov., Cronopiophrynus gen. nov., Gabophrynus gen. nov., Gentiloprynus gen. nov., Girondophrynus gen. novo Accordingly, we proposed 44 nomenclatural changes. Our results showed that the diversity of this group could be greater, therefore, we highlight that populational and phylogeographic studies of Phryninae are important / A subfamília Phryninae (Arachnida: Amblypygi) não possui uma revisão recente. Isso traz muitas dificuldades na identificação das espécies. Nenhuma hipótese filogenética para a subfamília foi proposta. A nomenclatura dos caracteres diagnósticos não é uniforme e a maioria das ilustrações não é suficientemente detalhada. Aqui, nós revisamos a subfamília Phryninae, redescrevemos as espécies conhecidas e seis espécies novas, e propomos uma nomenclatura uniforme para os dentes das quelíceras e espinhos dos pedipalpos. Nós realizamos uma análise filogenética usando evidência total e otimização direta no programa POY. Construímos uma matriz morfológica de 92 terminais e 174 caracteres, e uma matriz molecular usando 1557 pb (marcadores COI, l2S e 16S). Os dois conjuntos de informação foram analisados separadamente para perceber a influência de cada um deles na análise de evidência total. Os resultados das três análises foram diferentes. A análise morfológica não recuperou a subfamília Phryninae como monofilética, resultando em 90 topologias igualmente parcimoniosas, Porém, a árvore de consenso estrito teve uma boa resolução. A análise molecular recuperou Phryninae como monofilética, embora não tenha recuperado a família Phrynidae. A análise de evidência total permitiu obter uma única hipótese mais parcimoniosa a qual inclui todas as espécies de Phrynidae, e permitiu resolver as politomias obtidas na análise morfológica. Nesta hipótese, tanto Phrynidae como suas subfamílias se mantiveram monofiléticas. Em todos os resultados, os gêneros de Phryninae são polifiléticos. A árvore da análise de evidência total foi selecionada para elaborar uma nova proposta taxonômica. Mantivemos os gêneros Acanthophrynus, Phrynus e Paraphrynus e criamos cinco gêneros novos: Caicedophrynus gen. nov., Cronopiophrynus gen. nov., Gabophrynus gen. nov., Gentilophrynus gen. nov., e Girondophrynus gen. novo Propusemos 44 mudanças nomenclaturais, Nossos resultados sugerem que a diversidade do grupo é maior do que a conhecida. Isso nos faz considerar importante análises populacionais e filogeográficas em Phryninae

Os Cubozoa são animais de águas tropicais e subtropicais ao redor do mundo. A cubomedusa Chiropsalmus cf. quadrumanus (Cubozoa: Chiropsalmidae) habita na costa Atlântica americana entre os 35°N e 27°S, uma área com barreiras putativas que poderiam impedir a distribuição contínua de C. cf. quadrumanus, tornando-se um modelo interessante para estudos de estrutura populacional e filogeografia. O objetivo deste trabalho foi analisar a estrutura haplotípica de quatro populações da C. cf. quadrumanus da costa brasileira. Nossas análises foram baseadas nos marcadores mitocondriais COI e 16S e a região nuclear ITS de 40 indivíduos provenientes de Ceará (03°43S 038°29W); Rio de Janeiro (22°37S 041°54W); São Paulo (23°26S 45°04 W) e Paraná (25°41\'S 048°25\'W), assim como por estudos morfométricos de 5 indivíduos de cada população. Análises filogenéticas e de redes de haplótipos de cada marcador revelaram uma profunda divergência gênica entre duas linhagens, Ceará - São Paulo e Rio de Janeiro - Paraná, dados estes corroborados por alguns caracteres das analises morfométricas. Nossos resultados sugerem que a espécie Chiropsalmus cf. quadrumanus é um complexo de espécies resultado da combinação entre taxonomia conservadora e especiação críptica. Estudos mais abrangentes que contenham espécimes de outros pontos da costa brasileira, incluída a localidade tipo (Santa Catarina, Müller, 1859), poderão verificar a existência de outras possíveis espécies crípticas e ampliar o conhecimento sobre a distribuição das duas linhagens encontradas neste estudo. As conseqüências taxonômicas do presente trabalho são importantes e ainda deverão ser trabalhadas. A falta de informação sobre a espécie, em conjunto com atitudes taxonômicas conservadoras e eventos de especiação críptica, não nos permitem delimitar as novas espécies com clareza. De qualquer maneira, nossos resultados revelam que há um complexo de espécies com o nome Chiropsalmus quadrumanus no Atlântico Americano. O presente trabalho constitui o primeiro estudo filogeográfico para qualquer espécie de cubozoário. / Cubozoans live in tropical and subtropical waters around the world. Chiropsalmus cf. quadrumanus (Cubozoa: Chiropsalmidae) inhabits the Atlantic coast of America between 35°N and 27°S, an area with putative barriers that could prevent the continuous distribution of C. cf. quadrumanus, turning the species an interesting model to study population structure and phylogeography. The aim of this work was to analyze the haplotype structure of four populations of C. cf. quadrumanus from the Brazilian coast. We carried out our analyses based on the COI and 16S mitochondrial markers and the nuclear ITS region of 40 individuals from Ceará (03°43S 038°29W); Rio de Janeiro (22°37S 041°54W); São Paulo (23°26S 45°04 W) and Paraná (25°41\'S 048°25\'W), as well by morphometric surveys of 5 individuals from each population. Phylogenetic and haplotype network analyses of each marker reveled a deep genetic divergence between two lineages, Ceará - São Paulo and Rio de Janeiro - Paraná, this data were corroborated by some characters of the morphometrical analyses. Our results suggest that the species Chiropsalmus cf. quadrumanus is a complex of species resulting of the mixture of over-conservative taxonomy and cryptic speciation. More inclusive studies containing specimens from other points of the Brazilian coast, including the type locality (Santa Catarina, Müller, 1859),could verify the existence of other possible cryptic species and increase the knowledge about the distribution of the two lineages found in this study. The taxonomic consequences of this survey are important and should be overwrought. Lack of information about the species, conservative taxonomic attitudes and cryptic speciation events prevent us to delimit new species clearly. However, our results revealed a species complex named Chiropsalmus quadrumanus in the Atlantic coast of America. The present work represents the first phylogeographic approach of any cubozoan species.

Low light intensity habitats harbor unique sciophilous benthic communities and are a source of novel and unique sponge fauna. However, the community structure of these habitats is poorly studied to date. Thus, this study attempts to understand the composition and structure of sciophilous sponge populations in southeast Florida. Fifty limestone plates were placed on a shallow reef in Fort Lauderdale for two years (2010-2012). To identify the sponge community and their patterns over time, all plates were photographed at the end of each year. Then, samples were taken from each of the live sponge specimens observed on the plates and processed in the laboratory for taxonomical identification. A total of 45 different sponge species were found, the majority corresponding to the Poecilosclerida Order. Eighteen were identified to species level, twenty-two to genus, and five were undetermined. Eight sponges constitute new records to Florida, and four are potentially new species. The most dominant species include Oscarella sp.1, Dysidea etheria, Mycale sp.1, Halisarca caerula and Tedania ignis. Species richness significant varied among years, and species cover among sectors (inner and outer reef). However, sponge assemblages were similar between years with slightly variation between sectors. This study found a diverse and complex composition and structure of sponges that is quite distinct from the sponge assemblages on the open reefs. A guide of the biodiversity of cryptic sponge species was created to facilitate further studies in low light intensity habitats.

In temperate zone, especially Europe and North America, is the genus Geastrum very well inspected group of Gasteromycetes with long lasting and comprehensive history of research. Our knowledge of the diversity in other areas of distribution, especially in tropics, is more than limited. The aim of this diploma thesis is to contribute for cognition of diversity of this genus in Panama, Costa Rica and Colombia I have collected 86 samples belonging to genus Geastrum during three expeditions to Panama (Chiriqué province) in the years 2015, 2016, 2017. I have characterized those based on morphological and molecular data. I have also studied morphology of 40 borrowed herbarium specimens from Costa Rica, Colombia and Panama. I have successfully sequenced four locuses of DNA from my own samples (ITS, LSU, RPB1 and ATP6). This method had lover efficiency in herbarium specimens due to present contaminations, age of specimens or probably badly stored. Molecular phylogenetic analysis was carried out by Bayesian method and its results have in agreement with morphologic traits shown many lineages new for Panama and moreover at least four new species completely new to science. Variability in species complexes (e.g. G. lageniforme, G. saccatum, G. velutinum) is presented in more detail with emphasis to lineages...

Sexual reproduction entails complex co-evolution between the sexes, necessary for successful fertilization, ensuring individual and population-level fitness. Interfertility is the main criterion for species definition and understanding speciation requires detailed studies of reproductive barriers. However, many studies on reproductive barriers are constrained to infer evolutionary processes from patterns. In this thesis, I focus on a hybrid zone between collared and pied flycatchers (Ficedula albicollis and hypoleuca) on the island of Öland, and a trait that is essential for fertilization: sperm. Long-term monitoring of these species, combined with recent advances in molecular tools, allow me to study how complex on-going intersexual and interspecific interactions influence reproductive isolation in this young hybrid zone. I start by exploring the links between pre- and postmating sexual selection within collared flycatchers (paper I and II). I show that secondary sexual characters and indirect mate-choice benefits are tightly linked to physiology (paper I), and that a male’s attractiveness and dominance status dictate which sperm traits are optimal, as a male’s fertilization success depends on an interaction between sperm and display traits (paper II). I then report a source of strong postzygotic isolation between recently diverged collared and pied flycatchers: impaired spermatogenesis resulting in absence of mature sperm cells in hybrid males (paper III). I show however that pied flycatcher females, who are most exposed to hybridization, can mitigate these costs through mechanisms of cryptic female choice impairing heterospecific sperm performance, allowing them to bias paternity towards pure-species offspring (paper IV). Finally, by exploring the testes transcriptomes and sperm proteomes of both species, I highlight the importance of gene and protein regulation mechanisms in facilitating phenotypic divergence between these species (paper V). Thus, my thesis reveals complex interactions between primary and secondary sexual characters in a wild bird and suggests that mechanisms of sexual selection are tightly linked to essential physiological functions. I also show that genetic incompatibilities can evolve rapidly despite low genome-wide levels of divergence but that divergence in regulatory regions and proteins potentially allows fast evolution of molecular mechanisms impairing or preventing costly heterospecific fertilization.

A família Tephritidae congrega várias espécies de moscas-das-frutas que utilizam frutos como substrato alimentar no estágio larval, adquirindo o status de inseto-praga quando esses frutos são de valor comercial. O gênero Anastrepha é endêmico do Continente Americano e compreende cerca de 212 espécies descritas, das quais 109 ocorrem no Brasil. A espécie nominal Anastrepha fraterculus representa um complexo de espécies crípticas e se encontra distribuída pela Região Neotropical e sul dos Estados Unidos. No Brasil, através do estudo de diversas características biológicas e do marcador molecular ITS-1 (espaçador ribossômico nuclear), identificou-se a existência de três espécies crípticas no complexo fraterculus, a Anastrepha sp.1 affinis fraterculus, A. sp.2 aff. fraterculus e A. sp.3 aff. fraterculus. Marcadores gênicos presentes no DNA mitocondrial, como o gene cytochrome oxidase I (COI), são ferramentas amplamente utilizadas em análises filogenéticas, pois esta molécula apresenta características distintas do DNA nuclear, como o fato de possuir herança predominantemente materna, apresentar ausência ou baixíssima taxa de recombinação na maioria dos táxons, além de altas taxas mutacionais. Estas características possibilitam a obtenção de dados importantes na interpretação das relações entre as espécies. Amostras do complexo fraterculus (A. sp.1, A. sp.2, A. sp.3) de 14 localidades (média de 5 indivíduos / localidade) no sudeste do Brasil, uma amostra de A sp.4 do Equador e dois grupos externos (A. grandis e A. striata) foram utilizados. Fragmentos de 1139bp do gene COI foram amplificados e sequenciados, 45 haplótipos foram identificados: 30 em A. sp.1, 5 em A. sp.2 e 17 em A. sp.3. A distância média entre as espécies foi de 0,021 e o Fst médio foi 0,347 indicando estruturação populacional muito alta e pequena distância entre os haplótipos, que não apresentaram diferenças fixadas entre as espécies. Os testes de desvio de neutralidade apresentaram valores significativamente negativos. Os testes de seleção evidenciaram a atuação de seleção purificadora com baixos valores de Ka/Ks e significância no Z-teste de seleção. A análise filogenética mostrou fortes evidências de introgressão e não separou as diferentes entidades em clados distintos. Houve a formação de dois ramos principais, um constituído quase que exclusivamente por amostras de A. sp.1, e apenas duas amostras de A. sp.3, e outro que reuniu todas as espécies do complexo. Os dois principais grupos de haplótipos também foram visualizados na rede de haplótipos que mostrou indícios de expansão populacional. Quando somado ao estudo sequências depositadas em bancos de dados por outros autores, a espécie nominal A. fraterculus apresentou em sua distribuição 5 grupos de haplótipos mitocondriais. Dois deles ocorrem no Brasil, um com amostras do México e Costa Rica, um na Guatemala e Venezuela (baixa latitude) e um com indivíduos da Colômbia e Venezuela (alta latitude), sendo que os grupos Brasileiros também reuniram amostras da Argentina e do Equador. Assim, as sequências de COI não permitem a caracterização das entidades do complexo fraterculus apesar de indicar a estruturação populacional e a hipótese mais provável é a de que tenha havido introgressão da molécula mitocondrial entre as espécies do complexo com posterior expansão / The Tephritidae family comprises fruit flies species whose larvae feed and develop in fruits, many of which are commercial varieties and thus the species assume economic significance. Anastrepha genus is distributed throughout the Neotropical region and Southern United States. Analyses of biological characteristics and of the internal transcribed spacer (ITS-1) of the nuclear ribosomal DNA allowed the characterization of three cryptic species of the fraterculus complex in Brazil: Anastrepha sp.1 affinis fraterculus, Anastrepha sp.2 aff. fraterculus and Anastrepha sp.3 aff. fraterculus. Mitochondrial markers as gene cytochrome oxidase I (COI) are largely used in phylogenetic analyses because they have maternal inheritance, none or low recombination and high mutation rates compared to the nuclear DNA. Hence, analyses of the complex based in this marker will offer a divergent perspective from nuclear DNA for inferences on the evolutive relationships between different species. Samples from the fraterculus complex (A. sp.1, A. sp.2, A. sp.3) from 15 localities (average of 5 individuals/ locality) in southeastern Brazil, one sample of A. sp.4 from Ecuador and two outgroups (A. grandis and A. striata) were employed and COI sequences of 1139bp were amplified and analyzed. We identified 45 haplotypes: 30 in A. sp.1, 5 in A.sp.2 and 17 in A. sp.3. The mean distance between the haplotypes was 0.021 and mean Fst 0.347, indicating high population structure and low mitochondrial distance. The neutrality tests had significantly neutral values. The selection tests revealed the action of purifying selection with low values of Ka/Ks and significance in the Z-test selection. Phylogenetic analysis showed strong evidences of introgression and did not separate the various entities in distinct clades grouping the three species in a single branch; there was also the formation of another main branch formed almost exclusively by strains of A. sp.1 and only two samples of A. sp.3. The two main groups of haplotypes were also seen in the haplotype network that showed evidence of population expansion. The analysis of the philogenetic tree based on mitochondrial COI showed strong evidence for introgression. No fixed differences between species were found though mtDNA marker shows a lot of polymorphism. When added sequences deposited in databases by other authors the nominal species A. fraterculus presented in its distribution five groups of mitochondrial haplotypes, two of them in Brazil, one with samples from Mexico and Costa Rica, one in Guatemala and Venezuela and one with individuals from Colombia. The Brazilian groups also collected samples from Argentina and Ecuador. Therefore, the COI sequences do not allow the characterization of the entities of the fraterculus complex, although structure among the species is shown. The most likely hypothesis is that introgression has happened in the mitochondrial molecule among the species with further expansion

付記する学位プログラム名: 霊長類学・ワイルドライフサイエンス・リーディング大学院 / 京都大学 / 0048 / 新制・課程博士 / 博士(理学) / 甲第21616号 / 理博第4523号 / 新制||理||1649(附属図書館) / 京都大学大学院理学研究科生物科学専攻 / (主査)教授 岡本 宗裕, 准教授 Andrew MacIntosh, 教授 髙井 正成 / 学位規則第4条第1項該当 / Doctor of Science / Kyoto University / DGAM

The genus Ramariopsis (Clavariaceae, Agaricales) having ramarioid, rarely clavarioid basidiomata includes about 15 species in central Europe. They are probably saprotrophic species which usually occur in grasslands or rarely open places in shrubs or deciduous forests. In this work, 88 collections of this genus mainly from the Czech Republic and Slovakia were studied, of which 57 sequences from the LSU and 36 from the ITS regions of the nuclear ribosomal RNA gene complex were obtained. Based on molecular-phylogenetic analyses using the maximum likelihood and bayesian methods, phylogenetic trees were created showing 16 well-supported clades. So detailed study on the phylogeny and relationships among species of the genus Ramariopsis is presented for first time, even within the world literature. Subsequent morphological analysis supported by SEM study of basidiospores showed that obtained clades represent nine species known in taxonomic literature, three so far non-described species (that were labelled with provisional names) and four clades whose interpretation is uncertain at the moment. Thus, seven clades represent cryptic diversity, which is a rather high extent. A new species Ramariopsis robusta Matouš et Holec has already been published and Ramariopsis rufipes and Ramariopsis kunzei var. bispora...

[ES] El género de copépodos Tigriopus Norman, 1869 se distribuye en todo el mundo en piscinas costeras de rocas y actualmente se considera que incluye 15 especies válidas. Tigriopus fulvus (Fischer, 1860), con su subespecie Tigriopus fulvus adriaticus Van Douwe 1913 y Tigriopus fulvus algiricus Monard 1935, actualmente se informa que ocurren en el área mediterránea, pero actualmente se desconoce la diversidad real del género. Nuestro objetivo fue evaluar la identidad real de las poblaciones mediterráneas de Tigriopus y dilucidar su taxonomía y patrón de diversidad genética. Para alcanzar estos objetivos, utilizamos dos enfoques diferentes. El primero, basado en la morfología, donde las posibles diferencias morfológicas entre muestras topotípicas de Tigriopus fulvus s.s. y muestras topotípicas de las dos subespecies fueron investigadas. Un segundo, sobre una base molecular, donde se secuenciaron fragmentos de dos genes de ADN mitocondrial (subunidad I de citocromo c oxidasa, COI y subunidad de ARN ribosómico pequeño, 12S) y un gen de ADN nuclear (28S) para ser usados como marcador de referencia. En este marco, se utilizaron enfoques taxonómicos moleculares, como el descubrimiento automático de brechas de código de barras (ABGD), bPTP (procesos de árbol de Poisson bayesianos) y la relación K / ¿, para investigar la existencia de las supuestas subespecies de Tigriopus a través de la identificación de Operacional Unidades Taxonómicas (OTU). Nuestros datos sugieren la presencia de una sola especie caracterizada por una notable estructura genética basada en la geografía en toda el área de estudio. El patrón de diversidad observado se atribuye tentativamente aquí a una fuerte monopolización de los estanques de rocas por parte de los primeros inmigrantes que los alcanzan. Sin embargo, tal monopolización se ve interrumpida periódicamente por los eventos de extinción locales, que son frecuentes en los hábitats intrínsecamente inestables de las piscinas de rocas Aquí proponemos para este patrón el nombre de "monopolización periódica" ("Clockwork monopolization"). / [CA] El género de copépodos Tigriopus Norman, 1869 se distribuix en tot el mon en piscines costeres de roques i actualment se considera que inclou 15 especies valides. Tigriopus fulvus (Fischer, 1860), en la seua subespecie Tigriopus fulvus adriaticus Van Douwe 1913 i Tigriopus fulvus algiricus Monard 1935, actualment s'informa que ocorren en l'area mediterranea, pero actualment se desconeix la diversitat real del género. Nostre objectiu fon evaluar l'identitat real de les poblacions mediterranees de Tigriopus i dilucidar la seua taxonomia i patrón de diversitat genetica. Per a alcançar estos objectius, utilisem dos enfocaments diferents. El primer, basat en la morfologia, a on les possibles diferencies morfologiques entre mostres topotípicas de Tigriopus fulvus s.s. i mostres topotípicas dels dos subespecies foren investigades. Un segon, sobre una base molecular, a on se secuenciaron fragments de dos gens d'adn mitocondrial (subunidad i de citocromo c oxidasa, coi i subunidad d'arn ribosómico menut, 12s) i un gen d'adn nuclear (28s) per a ser amprats com marcador de referencia. En este marc, s'utilisaren enfocaments taxonomics moleculars, com el descobriment automatic de breches de codic de barres (ABGD), bPTP (processos d'arbre de poisson bayesianos) i la relacio K/¿, per a investigar l'existencia de les supostes subespecies de Tigriopus a través de l'identificacio d'operacional unitats taxonomiques (OTU). Nostres senyes sugerixen la presencia d'una sola especie caracterisada per una notable estructura genetica basada en la geografia en tota l'area d'estudi. El patrón de diversitat observat s'atribuix tentativament aci a una forta monopolisacio dels safarejos de roques per part dels primers immigrants que els alcancen. No obstant, tal monopolisacio se veu interrompuda periodicament pels events d'extincio locals, que son freqüents en els hábitats intrinsecament inestables de les piscines de roques aci proponem per a este patrón el nom de "Monopolisacio Periodica" ("Clockwork Monopolization"). / [EN] The copepod genus Tigriopus Norman, 1869 is distributed worldwide in coastal rock-pools and it is currently considered to include 15 valid species. Tigriopus fulvus (Fischer, 1860), with its subspecies Tigriopus fulvus adriaticus Van Douwe, 1913 and Tigriopus fulvus algiricus Monard, 1935, are currently reported to occur in the Mediterranean area, but the actual diversity of the genus is currently unknown. We aimed to assess the actual identity of Mediterranean Tigriopus populations and to elucidate their taxonomy and pattern of genetic diversity. In order to reach these goals, we use two different approaches. The first, based on morphology, where the possible morphological differences among topotypical samples of Tigriopus fulvus s.s. and topotypical samples of the two subspecies were investigated. A second, on a molecular basis, where fragments of two mitochondrial DNA genes (cytochrome c oxidase subunit I, COI and small ribosomal RNA subunit, 12S) and a nuclear DNA gene (28S) were sequenced to be used as a reference marker. In this frame, molecular taxonomical approaches, such as Automatic Barcode Gap Discovery (ABGD), bPTP (bayesian Poisson Tree Processes) and K/¿ ratio, were used in order to investigate the existences of the alleged subspecies of Tigriopus through the identification of Operational Taxonomic Units (OTUs). Our data suggest the presence of a single species characterized by a noteworthy geographically-based genetic structure in the whole study area. The observed diversity pattern is tentatively ascribed here to a strong monopolization of the rock pools by the first immigrants that reach them. However, such a monopolization is periodically disrupted by local extinction events, which are frequent in the intrinsically unstable rock pool habitats. We propose the name "clockwork monopolization" for this pattern. / Vecchioni, L. (2020). Systematics and phylogeography of the genus Tigriopus (Copepoda, Harpacticoida, Harpacticidae) in the basin of the Mediterranean Sea [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/144122 / TESIS