University, Diss., 2005--Bremen.

No description available.

Since the 18th-century scientists are studying diatoms, fascinated by their beauty and diversity. Their nano- and micropatterned biosilica cell walls are outstanding examples of biologically controlled mineral formation. Although the knowledge about diatom cell wall formation increased over the last 60 years, the process is still far away from being completely understood. Diatom cell walls exhibit highly interesting material properties, making them appealing to material scientists. Due to those properties, diatom cell walls are on the brink of becoming powerful tools in nanotechnology. However, the production of tailored silica structures for nanotechnology requires a much better understanding of the processes and components involved in cell wall morphogenesis. Recent studies set the focus on insoluble organic matrices as important parts of this process, suggesting that they act as templates in silica morphogenesis. Therefore, in this study, the occurrence of insoluble organic matrices and their possible silica precipitation activity was analyzed in the three diatom species T. pseudonana, T. oceanica and C. cryptica. For all three species girdle band and valve derived insoluble organic matrices could be identified. The extracted insoluble organic matrices exhibited structural features present in the corresponding biosilica cell walls. The highest similarities were found in the valve derived matrices of C. cryptica. Accessibility studies showed that the biosilica associated insoluble organic matrices of T. pseudonana were only partially accessible, arguing for an entrapment of insoluble organic matrices in the silica, rather than an attachment to the surface of the cell wall. All examined insoluble organic matrices of the three species exhibited intrinsic silica precipitation activity. The most intriguing structures were formed by the insoluble organic matrices of C. cryptica, yielding a porous silica pattern. The addition of biosilica derived soluble components or long-chain polyamines promoted this process and moreover lead to the reconstitution of biosilica-like hierarchical silica pore patterns. The generated silica structures were templated by the underlying structure of the insoluble organic matrix. The result presented in this thesis make this the first study reporting the in vitro generation of diatom biosilica-like hierarchical silica pore patterns using all natural cell wall components. It supports the hypothesis of microplates acting as templates for biosilica morphogenesis and introduces an interesting experimental setup for silica-based in vitro studies on the mechanism of pore formation in diatoms.

No description available.

Morphogenesis of the intricate patterns of diatom silica cell walls is a protein-guided process, yet to date only very few such silica biomineralization proteins have been identified. Therefore, it is currently unknown whether all diatoms share conserved proteins of a basal silica forming machinery, and whether unique proteins are responsible for the morphogenesis of species-specific silica patterns. To answer these questions, we extracted proteins from the silica of three diatom species (Thalassiosira pseudonana, Thalassiosira oceanica, and Cyclotella cryptica) by complete demineralization of the cell walls. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis of the extracts identified 92 proteins that we name ‘soluble silicome proteins’ (SSPs). Surprisingly, no SSPs are common to all three species, and most SSPs showed very low similarity to one another in sequence alignments. In-depth bioinformatics analyses revealed that SSPs could be grouped into distinct classes based on short unconventional sequence motifs whose functions are yet unknown. The results from the in vivo localization of selected SSPs indicates that proteins, which lack sequence homology but share unconventional sequence motifs may exert similar functions in the morphogenesis of the diatom silica cell wall.

Since 1750, human emissions of greenhouse gases have created a temperature increase, affecting the whole world. Diatoms, single-celled microscopic algae that lives in waters all over the world, have different environmental preferences such as the water depth at which the thrive, salinity, nutrients, pH-values and more. Diatoms also responds quickly to changing circumstances, which makes the suitable as environmental indicators. The purpose of the study is to investigate how the abundance and composition of diatoms has changed during the last 200 years in lake Ensjön, located just south of Norrköping, and to relate those changes both to climate change and known events and environmental factors in the area.  The survey is conducted by subsampling sediment cores from which each diatom sample was prepared in accordance with the scheme of Battarbee (1984). The diatoms in the samples were counted under a microscope and the results were analyzed and related to the Cyclotella-Aulacoseira-Fragilaria-theory (CAF) and documented historical events in the local environment. The result of the analysis shows that the composition and abundance of diatoms has changed during the last 200 years, and that this change is due to both climate change (temperature and precipitation increase) and the land-use history of Ensjön. The cahnges in composition of Aulacoseira spp. and Cyclotella spp., where Aulacoseira spp. is expected to decrease, and Cyclotella spp. is expected to increase with a rising temperature, match the CAF-theory for the most part. The change in composition also coincides with several documented environmental factors in the area, especially the change in use of fertilizers and herbicides in agriculture. / Människans utsläpp av växthusgaser har sedan år 1750 skapat en temperaturökning som går allt snabbare, och påverkar hela världen. Kiselalger, encelliga mikroskopiska alger som lever i vatten över hela världen, har olika miljöpreferenser såsom bland annat på vilket vattendjup de trivs, optimal halt av salt, näringsvärden, pH-värden, samt reagerar snabbt på förändrade omständigheter, vilket gör dem lämpliga som miljöindikatorer. Syftet med studien är att studera förändringar i kiselalgernas sammansättning under de senaste 200 åren i den lilla sjön Ensjön belägen något söder om Norrköping och att relatera dessa förändringar till dokumenterade händelser och miljöfaktorer i sjöns avrinningsområde samt till de pågående klimatförändringarna.  Undersökningen är utförd genom provtagning av sedimentkärnor från vilka prover preparerades enligt Battarbees (1984) flödesschema. Proverna av kiselalger räknades under mikroskop och sammansättningen analyserades utifrån teorin Cyclotella-Aulacoseira-Fragilaria (CAF) och kända historiska händelser i närmiljön. Analysresultatet visade att kiselalgssammansättningen har förändrats under de senaste 200 åren, och att denna förändring sannolikt kan kopplas både till klimatförändringar (en ökad temperatur) och Ensjöns markanvändningshistoria. Förändringen hos Aulacoseira spp. och Cyclotella spp. följer till stor del CAF-teorin om att det första släktet skulle minska och det andra skulle öka vid en temperaturökning. Vid flera tillfällen sammanfaller förändringar i kiselalgssammansättningen med kända miljöfaktorer i närmiljön, främst i samband med utvecklingen i användningen av mineralgödsel och växtskyddsmedel.