Autologous chondrocyte implantation (ACI) is a cell based therapy for the treatment of articular cartilage defects. Numerous studies have reported outcomes following ACI using a variety of patient reported outcomes (PROs), but no clear recommendations exist regarding which PRO is the most responsive to changes following ACI. Few studies have documented changes in performance based assessments (PBAs) following ACI. Response shift theory proposes that residual changes in self-report measures occur over time. Failing to account for response shift may result in over or under reporting of outcomes from which clinical decisions are made. The purposes of this dissertation were 1) review the literature concerning ACI outcomes to determine the responsiveness of PROs to changes in self-reported function following ACI, 2) evaluate the reliability of PBAs among ACI patients, 3) develop a descriptive timeline for the return of function 1 year following ACI using both PROs and PBAs, and 4) utilize PROs and PBAs to evaluate patients undergoing ACI for evidence of response shift. All PRO and PBA measures were collected preoperatively and 3, 6, and 12 months postoperatively. A retrospective then-test PRO evaluation of function prior to surgery was completed at 6 and 12 months. Response shift was calculated by subtracting the original pre-test score from the then-test score. A systematic review and meta-analyses of existing ACI outcome studies resulted in the recommendation of the International Knee Documentation Committee Subjective Knee Form (IKDC) and Lysholm Knee Scale as highly responsive PROs among ACI patients of varying activity levels. Despite significant increases in PRO scores as early as 6 months following ACI, improvement in PBAs at 12 months following ACI were limited to stride length, walking speed, and step-up force. Finally, no evidence of a group level effect for response shift was observed. These results support the validity of traditional pre-test/post-test research designs with no need to account for response shift when evaluating treatment effects of ACI on the group level. However, the Western Ontario and McMasters University Osteoarthritis Index (WOMAC) did show evidence of a measurable response shift on a patient by patient basis.

Mitochondria are the main sites for adenosine triphosphate (ATP) generation within most cells. Structural and functional alterations of mitochondria due to genetic abnormalities of mitochondria can cause respiratory chain dysfunction. In this study, the important role of mitochondria in energy metabolism was determined by comparing the effect of mitochondrial DNA (mtDNA) mutations on growth patterns and oxidative phosphorylation (OXPHOS) enzyme activities of six isolated clones (B5, B12, D4, D9, E1 and E8); as well as the effect of ATP supplement to culture using the slowest growing clone. The isolated clones had shown distinct growth pattern and morphology. The difference in proliferation rates among the clones was ascertained by the doubling times (B5=26.4h. B12=43.2h. D4=25.7h. D9=33.6h. E1=26.9h and E8=28.8h). The clone's slow growth rate was likely the result of mitochondrial mutations in the 16S rRNA gene, ND1, ND4, ND6 and COX III. Five heteroplasmic mutations were found in clone B12 (G2480T, C2513G, A2520T, C9527T and C14263G), one heteroplasmic mutation in clone D9 (A4137G) and one homoplasmic mutation in clone D4 (C11496). The mutations in clone B12 appeared to be deleterious to the cell by disrupting mitochondrial OXPHOS activities and reducing energy output. Additionally, extracellular ATP supplement to OXPHOS deficient clone B12 facilitated cell growth and enhances the gene expression. Increased expression of mtDNA-encoded respiratory chain complexes observed in clone B12 compared to clone D4 may reflect mitochondrial genomic adaptation to perturbations in cellular energy requirements. The stimulation of mitochondrial biogenesis may be a cellular response in compensation for defects in OXPHOS associated with mtDNA mutations. My data support the hypothesis that the variability in functional manifestations of mtDNA is attributed to the nature of the mutation, number of mutation and the gene specifically affected. These results will help to further our understanding of the relationship between mitochondrial mutation and cellular function.

Fotoninis kristalas sudarytas iš medžiagų su skirtingais lūžio rodikliais. Dėl stop juostos valdymas fotoniniame kristale yra diskutuotinas klausimas.Vienas iš metodų valdyti stop juostą, užpildyti jį skystuoju kristalu. užpildant opalą skystuoju kristalu, buvo sukurtas LC defektas. Keičiant elektrinį laiuką, intervale nuo 1V iki 6V, defektą galima valdyti. defekto poslinkis gautas per 4m.defektas opale juda šviesos greičiu 300 000 km/s, o stop juostoje greitis lygus 0m/s. / Photonic crystals (PCs) are periodic dielectric materials that can control the propagation of photons. Control of stop band position in photonic crystals was discussed.A method was developed to adjust the opal stop band position by controlling the refractive index.When filling in the opal LC the result was a LC defect. Changing the electric field wichin the interval from 1 V to 6 V it is possible handle the defect. The movement of the defect was 4nm. The defect in the opal in moving with light speed that is 300 000 km/s and the speed on the bang is 0 m/s.

Software defect prediction is motivated by the huge costs incurred as a result of software failures. In an effort to reduce these costs, researchers have been utilising software metrics to try and build predictive models capable of locating the most defect-prone parts of a system. These areas can then be subject to some form of further analysis, such as a manual code review. It is hoped that such defect predictors will enable software to be produced more cost effectively, and/or be of higher quality. In this dissertation I identify many data quality and methodological issues in previous defect prediction studies. The main data source is the NASA Metrics Data Program Repository. The issues discovered with these well-utilised data sets include many examples of seemingly impossible values, and much redundant data. The redundant, or repeated data points are shown to be the cause of potentially serious data mining problems. Other methodological issues discovered include the violation of basic data mining principles, and the misleading reporting of classifier predictive performance. The issues discovered lead to a new proposed methodology for software defect prediction. The methodology is focused around data analysis, as this appears to have been overlooked in many prior studies. The aim of the methodology is to be able to obtain a realistic estimate of potential real-world predictive performance, and also to have simple performance baselines with which to compare against the actual performance achieved. This is important as quantifying predictive performance appropriately is a difficult task. The findings of this dissertation raise questions about the current defect prediction body of knowledge. So many data-related and/or methodological errors have previously occurred that it may now be time to revisit the fundamental aspects of this research area, to determine what we really know, and how we should proceed.

Nanostructures are promising building blocks for quantum technologies due to their reproducible nature and ability to self-assemble into complex structures. However, the need to control these nanostructures represents a key challenge. Hence, this thesis investigates the manipulation and creation of quantum states in certain nanostructures. The results of this thesis can be applied to quantum information processing and to extremely sensitive magnetic-field measurements. In the first research chapter, we propose and examine methods for entangling two (remote) nuclear spins through their mutual coupling to a transient optically excited electron spin. From our calculations we identify the specific molecular properties that permit high entangling power gates for different protocols. In the next research chapter, we investigate another method to create entanglement; this time between two remote electronic spins. This method uses a very sensitive magnetic-field sensor based on a crystal defect that allows the detection of single magnetic moments. The act of sensing the local field constitutes a two-qubit projective measurement. This entangling operation is remarkably robust to imperfections occurring in an experiment. The third research chapter presents an augmented sensor consisting of a nitrogen-vacancy centre for readout and an `amplifier' spin system that directly senses tiny local magnetic fields. Our calculations show that this hybrid structure has the potential to detect magnetic moments with a sensitivity and spatial resolution far beyond that of a sensor based on only a nitrogen-vacancy centre, and indeed this may be the physical limit for sensors of this class. Finally, the last research chapter investigates measurements of magnetic-field strength using an ensemble of spin-active molecules. Here, we describe a quantum strategy that can beat the common standard strategy. We identify the conditions for which this is possible and find that this crucially depends on the decoherence present in the system.

Context. Software defect prediction aims to reduce the large costs involved with faults in a software system. A wide range of traditional software metrics have been evaluated as potential defect indicators. These traditional metrics are derived from the source code or from the software development process. Studies have shown that no metric clearly out performs another and identifying defect-prone code using traditional metrics has reached a performance ceiling. Less traditional metrics have been studied, with these metrics being derived from the natural language of the source code. These newer, less traditional and finer grained metrics have shown promise within defect prediction. Aims. The aim of this dissertation is to study the relationship between short Java constructs and the faultiness of source code. To study this relationship this dissertation introduces the concept of a Java sequence and Java code snippet. Sequences are created by using the Java abstract syntax tree. The ordering of the nodes within the abstract syntax tree creates the sequences, while small sub sequences of this sequence are the code snippets. The dissertation tries to find a relationship between the code snippets and faulty and non-faulty code. This dissertation also looks at the evolution of the code snippets as a system matures, to discover whether code snippets significantly associated with faulty code change over time. Methods. To achieve the aims of the dissertation, two main techniques have been developed; finding defective code and extracting Java sequences and code snippets. Finding defective code has been split into two areas - finding the defect fix and defect insertion points. To find the defect fix points an implementation of the bug-linking algorithm has been developed, called S + e . Two algorithms were developed to extract the sequences and the code snippets. The code snippets are analysed using the binomial test to find which ones are significantly associated with faulty and non-faulty code. These techniques have been performed on five different Java datasets; ArgoUML, AspectJ and three releases of Eclipse.JDT.core Results. There are significant associations between some code snippets and faulty code. Frequently occurring fault-prone code snippets include those associated with identifiers, method calls and variables. There are some code snippets significantly associated with faults that are always in faulty code. There are 201 code snippets that are snippets significantly associated with faults across all five of the systems. The technique is unable to find any significant associations between code snippets and non-faulty code. The relationship between code snippets and faults seems to change as the system evolves with more snippets becoming fault-prone as Eclipse.JDT.core evolved over the three releases analysed. Conclusions. This dissertation has introduced the concept of code snippets into software engineering and defect prediction. The use of code snippets offers a promising approach to identifying potentially defective code. Unlike previous approaches, code snippets are based on a comprehensive analysis of low level code features and potentially allow the full set of code defects to be identified. Initial research into the relationship between code snippets and faults has shown that some code constructs or features are significantly related to software faults. The significant associations between code snippets and faults has provided additional empirical evidence to some already researched bad constructs within defect prediction. The code snippets have shown that some constructs significantly associated with faults are located in all five systems, and although this set is small finding any defect indicators that transfer successfully from one system to another is rare.

Loss of bone in the mandible as a result from for example resection of bone tumors or trauma, can in more complex cases be reconstructed using a reconstruction plate to provide stability between the remaining mandible stumps. Different studies on reconstruction plates present a fracture rate of 2.8-9.8 %. The rate of plate fracture and plate loosening increases the need to improve the design of the reconstruction plate. A useful tool to find new designs for structures is topology optimization. Topology optimization is a mathematical based method where it is possible to define an optimization problem for a specific load case. Based on the defined problem, the solver calculates the most appropriate design to reach the final goal. The aim of this work is to investigate, describe, and discuss how new designs for reconstruction plates used for bridging large mandibular defects can be achieved by using topology optimization as a tool.   Two software programs handling topology optimization from Altair Engineering were used: SimLab 14.0 and HyperMesh 14.0. Both of them uses the solver OptiStruct to solve the defined topology optimization problem. The topology optimization problem was defined to minimize the compliance of the structure with an upper limit of the allowed volume fraction used for the new design. Three different clenching tasks were examined: right unilateral clench, clenching in the intercuspal position, and incisal clench. All three load cases resulted in different designs, the designs were also affected by the initial amount of screws used, and by the defined value on the allowed thickness of the created parts in the new design. The results gave an initial understanding of topology optimization, and indicated the possibilities a design process with topology optimization has to achieve new designs for reconstruction plates used for large mandibular fractures.

This study investigated the interactions of facially deformed infants (FD) with their mothers compared to a facially nondeformed control group (FND). All mother-infant dyads were videotaped for 10 minutes during a free play period. Mothers were instructed to spend time with their baby as they normally would. The videotaped interactions of 14 FD dyads and 14 FND dyads were rated by five raters for quality of interactions, amount of vocalization, touch, and face-to-face gaze. The infants were rated on their level of attractiveness from polaroid pictures and videotapes. Mothers also completed a questionnaire which assessed their infants' temperament. Three of the studies' four hypotheses were confirmed. First, the more attractive an infant was, the better his/her interactions with the mother were judged to be. Second, FD infant dyads were rated as significantly poorer in quality of interaction than FND dyads, although FD* dyads did not spend significantly less time vocalizing, touching, or in face-to-face gaze as predicted. A significantly higher percentage of FD infants were judged as having difficult temperament relative to FND infants. Finally, as predicted it was found that infants with difficult temperaments were more likely to exhibit poorer quality interactions than infants with less difficult temperaments. These results have important implications for providing anticipatory guidance to caregivers of FD infants. Without intervention, FD infants appear at risk for subsequent developmental problems stemming from disrupted early mother-infant interactions. Future research should focus on these interactions soon after the infant's birth, attempt to determine if FD infants' emotions can be reliably understood from their facial expressions (as has been found in normal infants) and extend the current research paradigm to include fathers of FD infants.

This thesis uses the techniques of atomic force microscopy (AFM) and conductiveAFM (CAFM) to study defect sites on GaN films. In particular, these defect sites demonstrate current leakage under reverse-bias conditions that are detrimental to device fabrication. Two growth techniques that were used to improve this leakage behavior for samples in this study included: epitaxial lateral overgrowth (ELO) and nano-ELO using a Si3N4 film. Both techniques decrease defects such as threading dislocations by controlling the nucleation and growth behavior of the GaN films. The EL0 technique uses a patterned dielectric film to laterally grow micron-wide regions (referred to as 'wings') that minimize dislocation defects. Our CAFM studies indicate that ELO films have no detectable leakage sites in these wing regions; however, between these regions the films have typical leakage site densities seen for standard films on the order of 107cm-3. The nano-ELO technique utilizes a porous Si3N4 film to reduce defects over the entire film, and CAFM data indicate nearly a factor of ten reduction in leakage site densities. The nano-ELO technique is therefore optimal for an overall improvement in film quality, whereas the ELO technique is suitable for device fabrication in patterned regions with optimized film quality.

- Products Liability in Czech and American Law The main purpose of my thesis is to describe and compare certain aspects of products liability in the Czech legal system and the American one. The thesis is divided into four chapters, the fourth being the center part devoted to analyzing individual terms both systems have in common. To be able to compare provisions relevant to products liability, the first chapter deals with pecuniary and non-pecuniary damage and conditions of liability for such damage in its first subchapter. The second subchapter explains the term defect and further discusses the obligation of proper performance when selling products and possible liability for a defective product. Third subchapter then contrasts these two types of liability and clarifies the differences between them. Chapter two briefly examines the progression of products liability in the Czech legal system beginning with the Act No. 59/1998 Coll., Products Liability Act, followed by mentioning the relevant European directive and finally focusing on the new civil code soon to become effective. The third chapter contains basic information on American products liability as a whole and explores the development of this type of liability through the course of American history. For a better understanding of products...