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Yes / The polysialyltransferases are biologically important glycosyltransferase enzymes responsible for the biosynthesis of polysialic acid, a carbohydrate polymer that plays a critical role in the progression of several diseases, notably cancer. Having improved the chemical synthesis and purification of the fluorescently-labelled DMB-DP3 acceptor, we report optimisation and validation of a highly sensitive cell-free high-throughput HPLC-based assay for assessment of human polysialyltransferase activity.

21世紀是數位化時代，網路傳播、數字電視、電子報刊、各種傳統媒體都進軍數位化市場。在科技的整合下，通訊、資訊、廣播已發展成為三位體，廣播不再是單一節目的音訊，透過數位媒介可以結合多媒體傳輸，DAB數位音訊廣播也演化成為DMB數位多媒體廣播，傳播高品質聲音並能傳輸影像、圖文等，替廣播帶來多元化的服務，數位廣播因此被稱為「看得到的聲音」”Visual Voice”或「豐富化聲音」” Rich Voice”。然而，國內數位廣播產業的發展已瀕臨絕境，業者紛紛棄收，唯一存活業者正奮力企盼找出活路。本研究經由對個案進行系統化分析，找出國外範例成功之關鍵因素，建立可能之營運模式，以供業者參考。 本研究以目前發展最為活絡之韓國DAB/DMB個案：T-DMB陣營與TU Media為探討個案，以波特五力分析找出其成關鍵因素，並藉此建立可能之營運模式與產業鏈。以此為基礎審視國內DAB數位廣播發展現況與唯一仍在運作的寶島新聲數位廣播為個案，找出其定位以及可能之營運模式。 本研究經由波特五力分析並歸納發現，韓國DAB/DMB個案：T-DMB陣營與TU Media成功關鍵因素包括：獨特內容、通路、合理價格、廣播訊號覆蓋率佳以及政策創造良好產業環境。其營運模式為跳脫原先廣播業者的定位，將自己定位為內容聚集商或平台營運商，結盟電信業、內容提供商、通路商、接收機製造商等成為一網絡關係系統，並且藉由電信業已架構完整之商務營運平台進行內容訂閱收費制度，開創新收入來源，廣播業者收入不僅限於廣告費。因之，以本研究之國內個案-寶島新聲數位廣播電台而言，其欲成功推展DAB數位廣播業務須借鏡韓國模式，重新定位，並擴大原網絡系統，與產業上下游相關業者，甚至於跨產業結盟。另一方面，政策也應重新定義數位廣播產業在國內數位化計劃的角色，找出適切之政策，以創造良好之產業環境。 / The 21st century is the digital age, the internet, the digital television, the electronic publication, traditional media is going digital. The “chips” changed out live. Under the digital convergence , the communication, the information, the broadcast have been converged. The DAB/DMB is based on the digital technology, and therefore can converged physically with the digitalized communications infrastructure . The digitalized broadcast are not only the voice programme , also multimedia which transmitting video, jpg files, text etc. Therefore, the DAB is called” Visual Voice” or “ Rich Voice”. However, our DAB broadcast industry is dying , the only operator , TNT DAB Radio Station which is the smallest DAB broadcaster is struggling to survival. In view of this, this research will focus on the South Korea cases :T-DMB league and TU Media which are the success model in the world. This research will analyze the business model, industry value chain, key success factors by Porter 5 force analysis and Competitive Advantage. Base on this, this research tries to find the success way of TNT DAB Radio Station. According to the service model of the T-DMB league and TU Media , this study discover the key success factors including unique content, distribution channels, affordable price, broadcast signal quality coverage and policy creating good industry environment. The T-DMB league and TU Media reposition and play the role of operator in the industry. Also they play the content aggregator. The T-DMB league and TU Media leverage to receiver manufactures, distribution channels and provide “Broadcasting and Communication Convergence Services”. The profit model is the content subscription and advertisement fee . Because of it, TNT DAB Radio Station should re-define the strategy- not broadcaster, should be service operator or content aggregator and build up the alliance. On the other hand, the government also should re-think the broadcast policy and create the good industrial environment.

本文主要探討的是韓國自90年代以來，公廣在特定的歷史結構下，當進入數位化後，政府在政策邏輯和KBS實際因應策略的發展歷程。從韓國公共廣電發展的歷史經驗來看，KBS從80年代便建立起垂直壟斷的廣電結構。此結構的形塑過程，涉及了各利益團體在不同權力多寡的互動；包括國家這個唯一大股東、政策制定者、美國政府與新興科技產業的涉入、韓國電器大廠、廣電經營業者和內部工會成員。這些參與者歷經衝突、談判、協調，而達成最終的妥協，也就是KBS在各個發展層面的優勢地位。即便進入數位化時代，革命性的科技變革，導致各國公廣皆面臨自身存在正當性的搖搖欲墜；身為廣電大國的英國，甚至採取市場結構重整的作法，以因應數位化挑戰。然而，韓國的公共廣電，卻仍在韓國歷史時空背景所形塑出的同一個特定結構中，進行角色轉換；而轉換新角色所需的工具，則是偶然歷史機遇下所產出的DMB─數位多媒體廣播服務。 因此，推動T-DMB，並不是KBS自行決定的數位化發展途徑，T-DMB亦非KBS維繫正當性地位的必要策略，而是能使KBS突破原有科技困境的「數位行動接收」這項技術。但此科技困境，卻也是整個歷史結構的發展結果，使公廣與政府之間的主從關係失去變動的可能性，而導致後來出現特定的數位化困境、以及隨之而生的特定因應模式。

<p>The increasing data rates in digital television networks increase the demands on data capacity of the current transmission channels. Through new standards, the capacity of exisiting channels is increased with new methods of error correction coding and modulation.</p><p>This thesis presents the design and implementation of a modulator for transmission of digital terrestrial television according to the Chinese DTMB standard.</p><p>The system is written in VHDL and is intended for implementation on an FPGA.</p>

The increasing data rates in digital television networks increase the demands on data capacity of the current transmission channels. Through new standards, the capacity of exisiting channels is increased with new methods of error correction coding and modulation. This thesis presents the design and implementation of a modulator for transmission of digital terrestrial television according to the Chinese DTMB standard. The system is written in VHDL and is intended for implementation on an FPGA.

This thesis deals with an analysis of a system called Digital Audio Broadcast and describes its mathematical model created in MATLAB program. In the first part basic blocks of a transmitter and a receiver are analyzed along with their principles. Then different types of transmission channels for static, portable and mobile receivers are described. The second part contains description of the DAB mathematical model itself. Individual blocks are described in the order of the DAB signal path, from the transmitter through the transmission channel to the receiver side. In this part the graphical user interface is also described. In the final part the results of experimental measurements and simulations are presented.