Thesis (M.S.)--West Virginia University, 2003. / Title from document title page. Document formatted into pages; contains vii, 104 p. Vita. Includes abstract. Includes bibliographical references (p. 68-72).

The energy drink market increased 240% between the years 2004 and 2008 and is expected to continue to grow. Growth of this market is concerning because energy drink consumption has been associated with a variety of health risk behaviors including increased marijuana use, sexual risk-taking, fighting, alcohol consumption, but little research has explored the relationship between energy drinks and dietary behaviors of emerging adults. Therefore, the purpose of this study was to explore the relationship between energy drink consumption and diet among a sample of emerging adults. Participants included 585 undergraduate students from a large, public US university (m age=18.7; 47% non-Hispanic White, 20.9% Hispanic, 25.5% Asian, 2.7% non-Hispanic Black, and 4.4% other; 56% Female). Using an online survey, students self-reported energy drink consumption and dietary behaviors. Past week and past month energy drink consumption was measured with single items asking the number of times and the number of days the student consumed an energy drink, respectively. Dietary behaviors measured included soda, diet soda, milk, sweet snacks, salty snacks, fruits, vegetables, breakfast, frozen food meals, fast food meals, and restaurant meals consumption in a usual week. Summing responses to items including weekly consumption of milk, fruits, vegetables, and breakfast created a healthy eating index. Similarly, summing responses on items including weekly consumption of soda, diet soda, sweet snacks, salty snacks, frozen food, fast food, and restaurant food created an unhealthy eating index. Linear regression analyses were run to determine the associations between energy drink consumption and the individual dietary behaviors as well as the healthy and unhealthy eating indices. All analyses controlled for gender, race/ethnicity, and body mass index (BMI). Additional models were run with gender as a moderator to determine if the associations between energy drink consumption and dietary behaviors differed by gender. Overall, 17.5% of students consumed energy drinks at least one time in the past week (12.6% male, 5.0% female), and 38.1% consumed energy drinks on at least one day in the past month (22.7% male, 15.4% female). Increased past month energy drink consumption was associated with a higher score on the unhealthy eating scale (B=0.25 (SE =0.13), p=0.001), soda consumption (B=0.11 (SE =0.05), p=<0.001), and diet soda consumption (B=0.07 (SE =0.04), p=0.007), and a lower score on the healthy eating scale (B=-0.27 (SE=0.17), p<0.05). Increased past week energy drink consumption was associated with a higher score on the unhealthy eating score (B=0.80 (SE =0.28), p=<0.001), soda (B=0.25 (SE =0.10), p=0.003), diet soda (B=0.20 (SE =0.09), p=0.003), pre-packaged salty snacks (B=0.16 (SE =0.09), p=0.04), and frozen meal consumption (B=0.16 (SE =0.05), p=<0.002). No moderation effect was found by gender. Results suggest that those students who consume more energy drinks also consume more unhealthy foods and beverages. Given these associations, it is important that interventions aimed at addressing diet quality, overweight, or obesity rates within this population also consider the implications of energy drink consumption as a risk factor. / text

The effects of a simulated in vitro digestion model on the viscosity of solutions of locust bean gum, guar gum, fenugreek gum, xanthan gum, gum Arabic, psyllium, flaxseed gum and soy soluble polysaccharides (SSPS) were examined in this study. All hydrocolloid solutions were formulated for low viscosity (LV), medium viscosity (MV) and high viscosity (HV), which were subsequently subjected to 3 treatments of equal volumes each. The treatments consisted of 1) H2O-dilutions, 2) acid and alkali in the absence of enzymes/bile and 3) an in vitro digestion model simulating the gastric and duodenal phases with pH changes in the presence of hydrolytic enzymes and bile salts. All hydrocolloids showed substantial reductions in viscosity, with dilutions exerting the greatest effect. Depending on the concentration, xanthan gum retained 20-50% of its initial viscosity while the other solutions were in a lower range of 1-16%, thereby showing considerable resilience to the 3 simulated conditions. / NSERC

The aim of this study was to understand the cellular mechanisms in enterocytes, which may decrease glucose uptake by viscous oat β-glucan. An in-vitro cell model examined the effect of diffusion limitation, fluid shear stimulation, and increased intestinal stretching. Mechanical stimulation of IEC-6 was assessed. A Flexcell Cell Streamer device applied different fluid flow stresses on cells. Flexcell FX-4000 was used for biaxial stretching of the cells. Following the confirmation of appropriate use of the cell model, the results indicated that high viscosity oat β-glucan might provide a physical barrier limiting diffusion of nutrients to the cells apical surfaces. Western blot analysis confirmed weak mechanical stimulation on the cells. Mechanical stimulation did not influence glucose uptake. Strain-induced cells showed lower activities in their glucose uptake. In conclusion, there may be a significant contribution of direct effects of the viscosity of oat β-glucan on cellular mechanisms of uptake in enterocytes.

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Six field pea (Pisum sativum) varieties from five different growing locations in Saskatchewan in the 2006 and 2007 growing years were analyzed to determine the effect of genotype, environment and year on the total dietary fibre, insoluble dietary fibre, soluble dietary fibre, total phenolic content, simple phenolic content and antioxidant activities. Samples were analyzed for dietary fibre using the enzymatic-gravimetric method of fibre analysis in accordance to the AACC method 32-05. Growing location had a very significant effect (p<0.0001) on the IDF, SDF and TDF content. Genotype had a strong effect (p<0.0001) on both IDF and TDF while having no significant effect (p=0.4556) on SDF content. Crop year also displayed a significant effect on SDF and TDF (p<0.0001) while having a smaller effect on IDF content (p=0.0139). Green varieties yielded significantly higher IDF (p=0.0041) and TDF (p=0.0028) than yellow varieties. Significant genotype x location (0.0155) and location x year (p=0.0002) interaction terms were also observed for TDF. The total phenolic contents were assessed using the Folin-Ciocalteu method of total phenolic content (TPC) analysis, while the contents of 10 individual simple phenolic acids were assessed using reversed-phase UPLC. A significant genotype, environment, and genotype by environment (G x E) interaction effect on the TPC was observed. The seed coat colour and growing season did not show a significant effect on the TPC. The UPLC analysis showed that ferulic acid comprised the majority of the phenolic content of the field pea samples. There was also a genotype, seed coat colour, location, growing season and G x E effect on the total simple phenolic acid content. As well, a modified microplate method for antioxidant activity using the free radical DPPH was assessed against the conventional cuvette method based system. Both methods showed that genotype (p<0.05) and location (p<0.05) had a significant effect on antioxidant activity. A larger, significant effect was seen in the genotype by environment (G x E) interaction (p<0.0001) in the 2007 and 2008 growing years. Growing year did not have a significant on antioxidant activity. Although there was some variation in the resulting AOA values between the two methods, these differences were found not to be statistically significant by means of a folded F-Test (p < 0.05), and the AOA between the two methods was highly correlated (R² = 0.8866). This indicates that a microplate may be used in place of cuvettes to determine AOA using the DPPH free radical to increase testing speed while reducing the amount of sample and reagent used in testing. The research performed on the influence of genotype and environment could potentially allow plant breeders, food scientists and nutraceutical manufacturers to manipulate field pea genotypes and growing conditions to attain an ideal nutritional profile for use in functional foods and nutraceuticals.

Replacement of saturated fatty acids (SFA) with monounsaturated fatty acids (MUFA) or polyunsaturated fatty acids (PUFA) impacts risk of atherosclerosis and cardiovascular disease (CVD). However, although dietary fatty acids (DFA) have been established as an important factor related to CVD, their exact mechanisms of action have not been clearly established. One of the possible mechanisms is that DFA convert to fatty acid ethanolamides (FAEs), such as oleoylethanolamide (OEA), palmitoylethanolamide (PEA) and arachidonoylethanolamide (AEA), which are thought to associate with lipid signalling, fat oxidation and appetite control. Hence, the objectives of this thesis were to identify the impact of diets containing corn oil, canola oil, DHA + canola oil and fish oil on plasma and organ levels of FAEs as well as energy metabolism and lipid profiles in Syrian Golden hamsters. Forty-eight hamsters were provided diets containing 6% treatment oil for 30 d before sacrifice. Across all diets, in proximal small intestine and liver, animals fed canola oil showed higher (p<0.05) levels of OEA than corn oil and fish oil fed groups, but no difference compared to those fed DHA +canola oil. In plasma, fish oil fed animals showed higher (p<0.05) OEA and PEA levels and lower (p<0.05) AEA levels compared to all other groups. Feed intakes (g/d), oxygen consumption (ml/g) and body composition of total fat (%) and mass (g) did not differ across groups. However, energy expenditure associated with fat oxidation (%) was higher (p<0.01) in canola oil and DHA + canola oil fed hamsters compared to those consuming corn oil and fish oil. Also, body composition of fish oil fed animals showed a lower (p<0.01) total lean mass (g) compared to other three groups and a lower (p<0.01) total mass (g) compared to DHA + canola oil diets, but no difference compared to animals fed the canola oil diet. None of the treatments had any effect on triglyceride (TG) or C-reactive protein (CRP) levels. The fish oil group showed a higher (p<0.01) plasma total cholesterol (TC) levels than all other three groups. No differences existed between DHA + canola oil and fish oil groups in HDL or Non-HDL levels, but these levels were different (p<0.01) compared to corn oil group and canola oil groups. To conclude, different DFA affect whole body energetics and plasma lipid profiles. Also DFA produced marked shifts in plasma and organ levels of OEA, PEA and AEA. These dietary induced shifts in FAEs may translate into discernable changes in energy expenditure and lipid levels which in turn influence CVD risk.

Dietary flaxseed has cardioprotective effects that may be achieved through its rich content of the omega-3 fatty acid, alpha linolenic acid (ALA). We investigated the effects of dietary flaxseed both with and without an atherogenic cholesterol-enriched diet to determine the effects of dietary flaxseed on the expression of the adipose cytokines leptin and adiponectin. Rabbits were fed one of four diets: a regular (RG) diet, or a regular diet with added 0.5% cholesterol (CH), or 10% ground flaxseed (FX), or both (CF) for 8 weeks. Levels of leptin and adiponectin expression were assessed by RT-PCR in visceral adipose tissue. Consumption of flaxseed significantly increased plasma and adipose levels of ALA. Leptin, but not adiponectin, mRNA expression was lower in CH animals and was elevated in CF animals. Changes in leptin expression were strongly and positively correlated with adipose ALA levels and inversely correlated with levels of en face atherosclerosis. Our data demonstrate that the type of fat in the diet as well as its caloric content can specifically influence leptin expression. The findings support the hypothesis that the beneficial cardiovascular effects associated with flaxseed consumption may be related to a change in leptin expression.

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Hand rearing infants has always carried a greater mortality rate than breast feeding despite the development of safe preserving techniques for milk: stool bacterial culture revealed two distinctive florae, fermentatitive, with a predominance of lactobacilli in breast fed infants and putrefactive, with a predominance of coliform bacteria in artificially fed infants. This difference was suggested to be the basis for the difference in the incidence of gastroenteritis. An artificial milk inducing a breast milk stool flora would, thus, reduce the incidence of gastroenteritis in artificially fed infants. The buffer capacity of milk was claimed to determine stool buffer capacity, which then encouraged growth of specific bacterial populations. However, an artificial milk with identical buffer capacity to breast milk returned disparate bacterial results. The hypothesis examined in this study was that buffer capacity of infant milk modifies stool flora. Breast milk and three artificial milks were studied. The artificial milks differed either in buffer capacity or in composition of major dietary components. Biochemical analyses were performed on milk, stool and stool water obtained by ultracentrifugation. Buffer capacity in milk was related to protein content. No biochemical differences were detected in stool water from each group, however whole stool buffer capacity was considerably greater in the group fed high buffer capacity milk than in the other three groups. No difference was detected in total bacterial numbers cultured, the types of organisms, nor individual species numbers. Thus the presently available highly modified artificial milks may determine stool buffer capacity, but do not induce a gut flora distinct from breast feeding. Thus the continuing increased incidence of gastroenteritis in artificially fed infants can no longer be related to differences.