Dieser Vortrag entspricht in seinem Inhalt dem ersten Kapitel des ZIN - Zertifikat Internet Nutzung der TU Chemnitz.

Evaluating the effect of exercise program DNS FIT KID in sportive older school-age children Abstract The diploma thesis evaluates of the effect of an exercise program called DNS FIT KID based on children older school-age who play sports. The theoretical part of the diploma thesis contains general knowledge about Dynamic neuromuscular stabilization. It describes the basic, such as developmental kinesiology, postural function and the stabilization system of the spine. Furthermore, the study also characterizes the DNS FIT KID exercise program, its key principles and exercise position. In the last section of the theoretical part of the diploma thesis, it approaches the specifics of the period of a child older school-age in sports. Methods: The research involved 15 sports participants (athletes and kayakers) aged 13 to 15 years. All participants attended 12 lessons of the DNS FIT KID exercise program. Once a week, the lessons took place under the guidance of a trained physiotherapist by the DNS method. Before and after the exercise program, the participants were examined using DNS FIT KID tests, gross motor tests and balance tests from the MABC-2 battery. Results: In DNS FIT KID tests, the results improved by an average of 11.2 points (p < 0,001), in tests from MABC-2 battery, the percentile of gross motor...

Understanding the interaction of polyelectrolytes with oppositely charged lipid membranes is an important issue of soft matter physics, which provides an insight into mechanisms of interactions between biological macromolecules and cell membranes. Despite the fact that many (bio)macromolecules and filamentous supramolecular assemblies show semiflexible behavior, prior to this work very little was known about the conformational dynamics and Brownian motion of semiflexible particles attached to freestanding lipid membranes. In order to address these issues, diffusion and conformational dynamics of semiflexible DNA molecules and filamentous fd-virus particles electrostatically adsorbed to cationic freestanding lipid membranes were studied on the single particle level by means of optical wide-field fluorescence microscopy. Supergiant unilamellar vesicles (SGUVs) with diameters larger than 100 m represent a perfect model of a freestanding membrane. In this work, a method was developed that enabled the reliable and efficient electroformation of cationic SGUVs on ITO-coated coverslips. The utilization of SGUVs as model freestanding lipid bilayers allowed for determination of the previously unknown surface viscosity of DOPC/DOTAP membranes. In particular, the analysis of the translational diffusion coefficients of small (10, 20, 50 nm) membrane-attached anionic polystyrene beads has shown that the surface viscosity of DOPC/DOTAP membranes with CDOTAP = 1–7 mol% is independent of the DOTAP concentration and equals η = (5.9 ± 0.2) × 10−10 Pa s m. The fluorescence video-microscopy investigation of single DNA molecules attached to cationic SGUVs revealed a previously unreported conformational transition of a membrane-bound DNA molecule from a 2D random coil, the original conformation in which DNA attaches to the membrane, to a compact globule. This membrane-mediated DNA condensation is favored at high cationic lipid concentrations in the membrane and long DNA contour lengths. The DNA compaction rate in the coil–globule transition is 124 ± 46 kbp/s, and the resulting DNA globule sizes were found to be 250–350 nm at DOPC membranes containing 1 mol% DOTAP and 130–200 nm for 7 mol% DOTAP, indicating a stronger compaction for higher charge densities in the membrane. Additional experiments with freestanding cationic membranes in the gel state and supported cationic lipid membranes with gel–fluid coexistence suggest that the DNA collapse on a freestanding fluid cationic membrane may be initiated by a local lipid segregation in the membrane and is accompanied by local membrane deformations, which eventually stabilize the compact DNA globule. Furthermore, in this work single molecule studies of random-coil DNA molecules and filamentous fd-virus particles on a freestanding cationic lipid bilayer with a low charge density were carried out. The experiments revealed that these particles can be described as semiflexible chains in 2D. Taken together, DNA molecules and fd-virus particles cover a broad range of the ratio of contour length and persistence length from 0.4 to 82. The results of this work demonstrate that the mobility of such membrane-attached semiflexible particles is strongly affected by hydrodynamics in the lipid membrane and the surrounding bulk fluid, and can in essence be described using a hydrodynamics-based theory for a disk-shaped solid membrane inclusion with a characteristic size approximately equal to the radii of gyration of the particles.

A fundamental problem in biology is the reconstruction of the relatedness of all (extant) species. Traditionally, systematists employ visually recognizable characters of organisms for classification and evolutionary analysis. Recent developments in molecular and computational biology, however, lead to a whole different perspective on how to address the problem of inferring relatedness. The discovery of molecules, carrying genetic information, and the comparison of their primary structure has, in a rather short period of time, revolutionized our understanding of the phylogenetic relationship of many organisms. These novel approaches, however, turned out to bear similar problems as previous techniques. Moreover, they created new ones. Hence, taxonomists came to realize that even with this new type of data not all problematic relationships could be unambiguously resolved. The search for complementary approaches has led to the utilization of rare genomic changes and other characters which are largely independent from the primary structure of the underlying sequence(s). These “higher order” characters are thought to be evolutionary conserved in certain lineages and largely unaffected by primary sequence data-based problems, allowing for a better resolution of the Tree of Life. The central aim of this thesis is the utilization of molecular characters of higher order in connection with their consistent and comparable extraction from a given data set. Two novel methods are presented that allow such an inference. This is complemented with the search for and analysis of known and novel molecular characteristics to study the relationships among Metazoa, both intra- as well as interspecific. The first method tackles a common problem in phylogenetic analyses: the inference of reliable data set. As part of this thesis a pipeline was created for the automated annotation of metazoan mitochondrial genomes. Data thus obtained constitutes a reliable and standardized starting point for all downstream analyses, e.g. genome rearrangement studies. The second method utilizes a subclass of gaps, namely those which define an approximate split of a given data set. The definition and inference of such split-inducing indels (splids) is based on two basic principles. First, indels at the same position, i.e. sharing the same end points in two sequences, are likely homologous. Second, independent single-residue insertions and deletions tend to occur more frequently than multi-residue indels. It is shown that trees based on splids recover most of the undisputed monophyletic groups while influence of the underlying alignment algorithm is relatively small. Mitochondrial markers are a valuable tool for the understanding of small and large scale population structure. The non-coding control region of mitochondrial DNA (mtDNA) often contains a higher amount of variability compared to genes encoding proteins and non-coding RNAs. A case study on a small scale population structure investigates the control region of the European Fire-bellied Toad in order to find highly variable parts which are of potential importance to develop informative genetic markers. A particular focus is placed on the investigation of the evolutionary dynamics of the repetitive region at an inter- and intraspecific level. This includes understanding mechanisms underlying its evolution, i.e. by exploring the impact of secondary structure on slipped strand mispairing during mtDNA replication. The 7SK RNA is a key player in the regulation of polymerase II (Pol-II) transcription, interacting with at least three known proteins: It mediates the inhibition of the Positive Transcription Elongation Factor b (P-TEFb) by the HEXIM1/2 proteins, thereby repressing transcript elongation by Pol-II. A highly specific interaction with LARP7 (La-Related Protein 7), on the other hand, regulates its stability. 7SK RNA is capped at its 5’ end by a highly specific methyltransferase MePCE (Methylphosphate Capping Enzyme). Employing sequence and structure similarity it is shown that the 7SK RNA as well as its protein binding partners have a much earlier evolutionary origin than previously expected. Furthermore, this study presents a good illustration of the pitfalls of using markers of higher order for phylogenetic inference.

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京都大学 / 0048 / 新制・課程博士 / 博士(工学) / 甲第21100号 / 工博第4464号 / 新制||工||1694(附属図書館) / 京都大学大学院工学研究科機械理工学専攻 / (主査)教授 黒瀬 良一, 教授 中部 主敬, 教授 吉田 英生 / 学位規則第4条第1項該当 / Doctor of Philosophy (Engineering) / Kyoto University / DFAM

The exceptional self-assembly properties of DNA as well as its ability to interact with different kinds of chemical compounds and biological structures make this biomolecule to an interesting object for the fabrication of artificial nanostructures. In this work several methods for a DNA-based self-assembly of electronic nanocircuitry are explored. For this, four basic steps, which turned out to be essential within a circuit assembly process, are addressed: (i) The formation of multi-branched DNA junctions by a simple building-block procedure. (ii) The site-specific attachment of nanoobjects (gold colloids) at the center of DNA junctions. (iii) The integration of DNA into microstructured gold electrode arrays, in particular the stretching of single DNA molecules between two electrodes. For this a simple, but reliable methods for the functionalization of gold electrodes by using aminoethanethiol was developed, which enables end-specific attachment of the DNA but does not require DNA modification. (iv) The metallization of DNA. A synthesis procedure was developed, which results in the formation of continuous chains of 5nm platinum clusters along the DNA. The metal deposition process turned out to take place exclusively at the DNA while background metallization is completely suppressed.

It is often desirable to study simulated turbulent flows at steady state even if the flow has no inherent source of turbulence kinetic energy. Doing so requires a numerical forcing scheme and various methods have been studied extensively for turbulence that is isotropic and homogeneous in three dimensions. A review of these existing schemes is used to form a framework for more general forcing methods. In this framework, the problem of developing a forcing scheme in Fourier space is abstracted into the two problems of (1) prescribing the spectrum of the input power and (2) specifying a force that has the desired characteristics and that adds energy to the flow with the correct spectrum. The framework is used to construct three forcing schemes for horizontally homogeneous and isotropic, vertically stratified turbulence. These schemes are implemented in large-eddy simulations and their characteristics analyzed. Which method is “best” depends on the purpose of the simulations, but the framework for specifying forcing schemes enables a systematic approach for identifying a method appropriate for a particular application.

Det säkerhetspolitiska läget i norra europa har stadigt försämrats sedan den ryska occupationen av Krim år 2014. Som ett resultat av detta ökar cyberattacker mot Sverige och resterande EU både i omfattning och frekvens. En av de största hoten är de cyberattacker som genomförs av aktörer som APT 28 och APT29 som har främmande staters stöd. Syftet med cyberattacker varierar stort dock är en av dessa åtkomsten till data/information. För att kunna uppnå det här målet måste aktören ha förmåga till att exfltrera data ur ett system eller nätverk. Det fnns fertalet metoder för att åstadkomma detta där en av metoderna är att exfltrera data över ett alternativt protokoll så som DNS-tunnling. Försvarsmakten nyttjar en rad olika system i syfte att uppnå sina mål och har särskilt utbildade enheter för att försvara dessa. Då varje användare indirekt bidrar till systemet och nätverkets säkerhet/skydd behöver samtliga användare ha en förståelse för hur deras användande påverkar systemet/nätverket. Skapandet av en DNS-tunnel ger användaren möjlighet att skicka ett protokolls (I det här fallet IPv4) data genom ett annat genom en process som heter inkapsling av data. Detta gör det svårare för system att automatiskt detektera illasinnad exfltration och således svårare att blokera trafken. Försvarsmakten bör regelbundet öva underrättelsefunktionerna i nyttjandet av STRIX och TAXII för att hantera hot i cyberdomänen och sprida dessa till övriga organisation. Detta skulle således möjliggöra för Cyberförsvaret att orientera resterande Försvarsmakt mer regelbundet och på så sätt även kunna anpassa de automatiska detektionsmetoder över tid för att bättre kunna fånga upp illasinnad exfltrering av data. Försvarsmakten bör utveckla underrättelsefunktionen så att den kan agera som en tolk mellan Cyberförsvaret och resterande Försvarsmakt. / The security in northern Europe has steadily become worse since the Russian occupation of Crimea in 2014. As a result of this the frequency and magnitude of hostile cyberoperations targeting Sweden and the EU has increased. One of the biggest threats are the cyberoperations that are carried out by state sponsored actors such as APT28 and APT29. The Cyberattacks can have a wide range of objectives, one of them being the acquisition of data/information. In order to accomplish that objective an adversary would require a method to exfltrate the data from the system or network. There are however multiple different methods that can be used, one being exfltration through an alternate protocol such as DNS-tunneling. The Swedish armed forces use a variety of different systems in order to accomplish its missions/tasks. In order to be able to defend the systems from any malicious activity the Swedish armed forces have specially trained units tasked specifically with the defnece of the armed forces systems. This however is not enough as every user in a system is part of that systems defence. This means that every service member must have an understanding of what implications activities in the cyber domain have upon activities in the other domains. The creation of a DNS-tunnel enables a user to send data of one protocol (in this case IPv4 data) over the DNS protocol through the process of encapsulation. This makes it harder for automated processes to detect the malicious exfltration and subsequently block the traffic. The Swedish armed forces should regularly train its intelligence community in order to handle cyber threat intelligence according to STIX and TAXII. This should allow the cyber defnece to more readily share their intelligence with the rest of the armed forces and use the established intelligence community as a translator that can deduce the implications of the threats on the day to day activity of the Army, Navy, Air Force and Home guard.

Temporally developing direct numerical simulations (T-DNS) are performed and validated for bypass transition of a zero pressure gradient flat plate boundary layer to understand the interplay between pressure-strain terms and flow instability mechanisms, and to propose and validate a phenomenological hypothesis for the identification of a robust transition onset marker for use in transition-sensitive Reynolds-averaged Navier-Stokes (RANS) computational fluid dynamics (CFD) simulations. Results show that transition initiates at a location where the slow pressure-strain term becomes more dominant than the rapid term in the pre-transitional boundary layer region. The slow pressure strain term is responsible for the transfer of turbulence energy from the streamwise component to other components while the rapid pressure strain term counteracts with the slow term in the pre-transitional regime before transition onset akin to a shear sheltering like effect. The relative magnitudes of the slow and rapid terms thus provide a basis for the development of physically meaningful large-scale parameters that can be used as a transition onset marker for Reynolds averaged Navier-Stokes RANS simulations.